FvH4_1g30370

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0008491
Physical Location & Seq
Forward (+)
24069555 .. 24072271
2717 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g30370.t2

Sequence Viewer

Length: 1071 bp
ATGGAAGCTGTATTCGGGATGGCTCTAGCCACCATTATTTTGACAAGCATGGCAGCCATGGCGGCATCAGCAGCATCTCCAACCACTTTTGTGTTCGGTGATTCATTAACAGATGTCGGAAACAACAATTTTTTGCAGTATTCTCTTGCAAAGTCGAATTATCCTTGGTATGGGGTCGATTATGAAGGTGGTCAGGCTACTGGAAGATTCACAAATGGAAGAACTGTTGGTGATATAATATCTGCGAAGCTTGGGATTTCATCACCGCCGCCTTACCTCTCGTTGTCAATGAATGATGATGCACTCCTTAAGGGGGTCAACTATGCATCTGGTGGAGCAGGAATACTAAATGACACCGGACTCTATTTTATTGAGAGATTGTCCTTCGACGATCAAGTACAAAGTTTCAACAAGACAAGGGAAGCCATCAAGGCTAAGATTGGGAGTGAAGCTGCAACCAAGCTCTTGACTGAAGCTCTCTATTTCATTGGAATTGGCAGCAATGATTATGTCAACAATTTCTTGCAACCCTTTTTACCCGATGGTCAAGAGTACACTCATGATGACTTTGTGGGACTGTTAATAGGCACCTTAGGGCAACAATTTCAGACGCTCTACCAGCTGGATGCGCGAAAGATGATATTTCACGGTCTTGGTCCTCTCGGCTGCATTCCTTCTCAGAGGGTTAAATCAAAGAAAGGCCAATGCTTGAAGGAAGTCAACCTGTGGGTGCTGGACTTCAACTCTAAAGTGGAGAAGCTCATTGCCTCTCTTAACAGACGCCTCCCGAGAGCAGATTTAACATTTGCAGATACATATTCAGATGTTCTCGACTTAATCAGCAACCCTACTGCATATGGGTTTAAGGTTTCCAATACGTCATGTTGCAAGGTAGACACACGGATTGGAGGGCTATGCTTACCGAATTCAAAACTATGCAAGAACCGCAAAGATTATGTATTCTGGGATGCATTCCACCCCTCAGATGCAGCGAATGTAGTTCTTGCTGACAAACTCTTCTCGAGACTATTTCCTAAAGCTTCCTCTCCTGCACCAACACTTTCTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

357

Amino Acids

38.81

Weight (kDa)

6.89

Isoelectric Point (pI)

25.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 30 - 274 1e-25 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 587
AccI GTMKAC 1 cut(s) 894
AccII CGCG 1 cut(s) 631
AciI CCGC 4 cut(s) 62, 266, 269, 946
AcsI RAATTY 1 cut(s) 925
AcuI CTGAAG 1 cut(s) 492
AcyI GRCGYC 1 cut(s) 781
AfaI GTAC 2 cut(s) 399, 554
AfiI CCNNNNNNNGG 2 cut(s) 170, 313
AflII CTTAAG 1 cut(s) 308
AgsI TTSAA 4 cut(s) 409, 712, 742, 930
AleI CACNNNNGTG 1 cut(s) 89
AluBI AGCT 8 cut(s) 8, 250, 452, 463, 476, 622, 760, 1040
AluI AGCT 8 cut(s) 8, 250, 452, 463, 476, 622, 760, 1040
Alw26I GTCTC 1 cut(s) 1018
Ama87I CYCGRG 2 cut(s) 787, 1021
AoxI GGCC 1 cut(s) 700
ApeKI GCWGC 6 cut(s) 53, 71, 452, 498, 666, 989
ApoI RAATTY 1 cut(s) 925
AspLEI GCGC 1 cut(s) 631
AspS9I GGNCC 1 cut(s) 656
AsuHPI GGTGA 3 cut(s) 110, 242, 255
AvaI CYCGRG 2 cut(s) 787, 1021
AvaII GGWCC 1 cut(s) 656
AxyI CCTNAGG 1 cut(s) 592
BanI GGYRCC 1 cut(s) 587
BbvI GCAGC 6 cut(s) 65, 83, 439, 510, 653, 1001
BccI CCATC 3 cut(s) 13, 434, 536
BcoDI GTCTC 1 cut(s) 1018
BfaI CTAG 1 cut(s) 26
BfrI CTTAAG 1 cut(s) 308
BglI GCCNNNNNGGC 2 cut(s) 62, 431
BisI GCNGC 8 cut(s) 54, 63, 72, 269, 453, 499, 667, 990
BlsI GCNGC 8 cut(s) 55, 64, 73, 270, 454, 500, 668, 991
Bme18I GGWCC 1 cut(s) 656
BmeT110I CYCGRG 2 cut(s) 787, 1021
BmgT120I GGNCC 1 cut(s) 656
BmiI GGNNCC 1 cut(s) 589
BmsI GCATC 7 cut(s) 74, 83, 289, 335, 616, 958, 976
BsaHI GRCGYC 1 cut(s) 781
BsaJI CCNNGG 2 cut(s) 57, 164
BsaWI WCCGGW 1 cut(s) 356
Bsc4I CCNNNNNNNGG 2 cut(s) 170, 313
Bse1I ACTGG 1 cut(s) 205
Bse21I CCTNAGG 1 cut(s) 592
Bse3DI GCAATG 2 cut(s) 508, 762
BseDI CCNNGG 2 cut(s) 57, 164
BseGI GGATG 3 cut(s) 24, 631, 973
BseLI CCNNNNNNNGG 2 cut(s) 170, 313
BseMI GCAATG 2 cut(s) 508, 762
BseMII CTCAG 2 cut(s) 692, 996
BseNI ACTGG 1 cut(s) 205
BseXI GCAGC 6 cut(s) 65, 83, 439, 510, 653, 1001
BsgI GTGCAG 1 cut(s) 1035
Bsh1236I CGCG 1 cut(s) 631
BshFI GGCC 1 cut(s) 702
BshNI GGYRCC 1 cut(s) 587
BsiHKCI CYCGRG 2 cut(s) 787, 1021
BsiSI CCGG 1 cut(s) 357
BslFI GGGAC 1 cut(s) 588
BslI CCNNNNNNNGG 2 cut(s) 170, 313
BsmAI GTCTC 1 cut(s) 1018
BsmFI GGGAC 1 cut(s) 588
BsmI GAATGC 2 cut(s) 669, 971
BsnI GGCC 1 cut(s) 702
BsoBI CYCGRG 2 cut(s) 787, 1021
Bsp143I GATC 1 cut(s) 391
Bsp19I CCATGG 1 cut(s) 57
BspACI CCGC 4 cut(s) 62, 266, 269, 946
BspANI GGCC 1 cut(s) 702
BspCNI CTCAG 2 cut(s) 691, 995
BspFNI CGCG 1 cut(s) 631
BspHI TCATGA 1 cut(s) 559
BspLI GGNNCC 1 cut(s) 589
BspT107I GGYRCC 1 cut(s) 587
BspTI CTTAAG 1 cut(s) 308
BsrDI GCAATG 2 cut(s) 508, 762
BsrI ACTGG 1 cut(s) 205
BssECI CCNNGG 2 cut(s) 57, 164
BssMI GATC 1 cut(s) 391
BssNI GRCGYC 1 cut(s) 781
BssT1I CCWWGG 2 cut(s) 57, 164
Bst4CI ACNGT 3 cut(s) 226, 579, 650
Bst6I CTCTTC 1 cut(s) 1022
BstACI GRCGYC 1 cut(s) 781
BstAFI CTTAAG 1 cut(s) 308
BstDEI CTNAG 4 cut(s) 435, 592, 678, 982
BstDSI CCRYGG 1 cut(s) 57
BstF5I GGATG 3 cut(s) 24, 631, 973
BstFNI CGCG 1 cut(s) 631
BstHHI GCGC 1 cut(s) 631
BstKTI GATC 1 cut(s) 394
BstMAI GTCTC 1 cut(s) 1018
BstMBI GATC 1 cut(s) 391
BstMWI GCNNNNNNNGC 8 cut(s) 59, 62, 68, 71, 431, 619, 628, 945
BstUI CGCG 1 cut(s) 631
BstV1I GCAGC 6 cut(s) 65, 83, 439, 510, 653, 1001
Bsu36I CCTNAGG 1 cut(s) 592
BsuRI GGCC 1 cut(s) 702
BtgI CCRYGG 1 cut(s) 57
BtsCI GGATG 3 cut(s) 24, 631, 973
CciI TCATGA 1 cut(s) 559
CfoI GCGC 1 cut(s) 631
Cfr13I GGNCC 1 cut(s) 656
CseI GACGC 2 cut(s) 619, 789
Csp6I GTAC 2 cut(s) 398, 553
CviAII CATG 4 cut(s) 49, 58, 560, 882
CviQI GTAC 2 cut(s) 398, 553
DdeI CTNAG 4 cut(s) 435, 592, 678, 982
DpnI GATC 1 cut(s) 393
DpnII GATC 1 cut(s) 391
Eam1104I CTCTTC 1 cut(s) 1022
EarI CTCTTC 1 cut(s) 1022
Eco130I CCWWGG 2 cut(s) 57, 164
Eco47I GGWCC 1 cut(s) 656
Eco57I CTGAAG 1 cut(s) 492
Eco81I CCTNAGG 1 cut(s) 592
Eco88I CYCGRG 2 cut(s) 787, 1021
EcoRI GAATTC 1 cut(s) 925
EcoT14I CCWWGG 2 cut(s) 57, 164
EcoT22I ATGCAT 2 cut(s) 328, 973
ErhI CCWWGG 2 cut(s) 57, 164
FaeI CATG 4 cut(s) 52, 61, 563, 885
FaqI GGGAC 1 cut(s) 588
FatI CATG 4 cut(s) 48, 57, 559, 881
FauNDI CATATG 1 cut(s) 856
FblI GTMKAC 1 cut(s) 894
Fnu4HI GCNGC 8 cut(s) 54, 63, 72, 269, 453, 499, 667, 990
FokI GGATG 3 cut(s) 31, 638, 980
Fsp4HI GCNGC 8 cut(s) 54, 63, 72, 269, 453, 499, 667, 990
FspBI CTAG 1 cut(s) 26
GlaI GCGC 1 cut(s) 630
GluI GCNGC 8 cut(s) 54, 63, 72, 269, 453, 499, 667, 990
HaeIII GGCC 1 cut(s) 702
HapII CCGG 1 cut(s) 357
HgaI GACGC 2 cut(s) 619, 789
HhaI GCGC 1 cut(s) 631
Hin1I GRCGYC 1 cut(s) 781
Hin1II CATG 4 cut(s) 52, 61, 563, 885
Hin6I GCGC 1 cut(s) 629
HinP1I GCGC 1 cut(s) 629
HincII GTYRAC 3 cut(s) 319, 514, 721
HindII GTYRAC 3 cut(s) 319, 514, 721
HindIII AAGCTT 2 cut(s) 248, 1038
HinfI GANTC 3 cut(s) 101, 207, 360
HpaII CCGG 1 cut(s) 357
HphI GGTGA 3 cut(s) 110, 242, 255
Hpy166II GTNNAC 5 cut(s) 319, 514, 555, 721, 895
Hpy188I TCNGA 5 cut(s) 119, 609, 681, 823, 985
Hpy188III TCNNGA 8 cut(s) 16, 466, 548, 560, 787, 830, 1021, 1023
Hpy8I GTNNAC 5 cut(s) 319, 514, 555, 721, 895
Hpy99I CGWCG 1 cut(s) 392
HpyAV CCTTC 4 cut(s) 179, 394, 684, 706
HpyCH4III ACNGT 3 cut(s) 226, 579, 650
HpyCH4IV ACGT 1 cut(s) 878
HpyF10VI GCNNNNNNNGC 8 cut(s) 59, 62, 68, 71, 431, 619, 628, 945
HpyF3I CTNAG 4 cut(s) 435, 592, 678, 982
HpySE526I ACGT 1 cut(s) 878
Hsp92I GRCGYC 1 cut(s) 781
Hsp92II CATG 4 cut(s) 52, 61, 563, 885
HspAI GCGC 1 cut(s) 629
Kzo9I GATC 1 cut(s) 391
LmnI GCTCC 1 cut(s) 335
Lsp1109I GCAGC 6 cut(s) 65, 83, 439, 510, 653, 1001
LweI GCATC 7 cut(s) 74, 83, 289, 335, 616, 958, 976
MaeI CTAG 1 cut(s) 26
MaeII ACGT 1 cut(s) 878
MalI GATC 1 cut(s) 393
MboI GATC 1 cut(s) 391
MboII GAAGA 3 cut(s) 216, 231, 1009
MluCI AATT 6 cut(s) 127, 157, 492, 517, 602, 925
MlyI GAGTC 1 cut(s) 354
MmeI TCCRAC 2 cut(s) 97, 104
MnlI CCTC 8 cut(s) 287, 669, 675, 778, 794, 902, 991, 1054
Mph1103I ATGCAT 2 cut(s) 328, 973
MseI TTAA 8 cut(s) 107, 309, 581, 687, 774, 800, 836, 864
MslI CAYNNNNRTG 1 cut(s) 89
MspA1I CMGCKG 1 cut(s) 622
MspCI CTTAAG 1 cut(s) 308
MspI CCGG 1 cut(s) 357
Mva1269I GAATGC 2 cut(s) 669, 971
MvnI CGCG 1 cut(s) 631
MwoI GCNNNNNNNGC 8 cut(s) 59, 62, 68, 71, 431, 619, 628, 945
NcoI CCATGG 1 cut(s) 57
NdeI CATATG 1 cut(s) 856
NdeII GATC 1 cut(s) 391
NlaIII CATG 4 cut(s) 52, 61, 563, 885
NlaIV GGNNCC 1 cut(s) 589
NmeAIII GCCGAG 1 cut(s) 642
NsiI ATGCAT 2 cut(s) 328, 973
OliI CACNNNNGTG 1 cut(s) 89
PaeR7I CTCGAG 1 cut(s) 1021
PagI TCATGA 1 cut(s) 559
PctI GAATGC 2 cut(s) 669, 971
PfeI GAWTC 2 cut(s) 101, 207
PkrI GCNGC 8 cut(s) 55, 64, 73, 270, 454, 500, 668, 991
PleI GAGTC 1 cut(s) 354
PpsI GAGTC 1 cut(s) 354
PspN4I GGNNCC 1 cut(s) 589
PspPI GGNCC 1 cut(s) 656
PvuII CAGCTG 1 cut(s) 622
RsaI GTAC 2 cut(s) 399, 554
RsaNI GTAC 2 cut(s) 398, 553
RseI CAYNNNNRTG 1 cut(s) 89
SaqAI TTAA 8 cut(s) 107, 309, 581, 687, 774, 800, 836, 864
SatI GCNGC 8 cut(s) 54, 63, 72, 269, 453, 499, 667, 990
Sau3AI GATC 1 cut(s) 391
Sau96I GGNCC 1 cut(s) 656
SchI GAGTC 1 cut(s) 354
SfaNI GCATC 7 cut(s) 74, 83, 289, 335, 616, 958, 976
Sfr274I CTCGAG 1 cut(s) 1021
SinI GGWCC 1 cut(s) 656
SlaI CTCGAG 1 cut(s) 1021
SmiMI CAYNNNNRTG 1 cut(s) 89
SmlI CTYRAG 2 cut(s) 308, 1021
SmoI CTYRAG 2 cut(s) 308, 1021
Sse9I AATT 6 cut(s) 127, 157, 492, 517, 602, 925
SsiI CCGC 4 cut(s) 62, 266, 269, 946
SspMI CTAG 1 cut(s) 26
StyI CCWWGG 2 cut(s) 57, 164
TaaI ACNGT 3 cut(s) 226, 579, 650
TaiI ACGT 1 cut(s) 881
TaqI TCGA 5 cut(s) 155, 177, 387, 831, 1022
TasI AATT 6 cut(s) 127, 157, 492, 517, 602, 925
TatI WGTACW 2 cut(s) 397, 552
TauI GCSGC 2 cut(s) 65, 271
TfiI GAWTC 2 cut(s) 101, 207
Tru1I TTAA 8 cut(s) 107, 309, 581, 687, 774, 800, 836, 864
Tru9I TTAA 8 cut(s) 107, 309, 581, 687, 774, 800, 836, 864
TseI GCWGC 6 cut(s) 53, 71, 452, 498, 666, 989
TspDTI ATGAA 5 cut(s) 93, 198, 249, 305, 475
TspGWI ACGGA 1 cut(s) 916
Vha464I CTTAAG 1 cut(s) 308
VpaK11BI GGWCC 1 cut(s) 656
XapI RAATTY 1 cut(s) 925
XhoI CTCGAG 1 cut(s) 1021
XmiI GTMKAC 1 cut(s) 894
XspI CTAG 1 cut(s) 26
Zsp2I ATGCAT 2 cut(s) 328, 973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.