Rw3G025870

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0008491
Physical Location & Seq
Forward (+)
34914615 .. 34916447
1833 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G025870.1

Sequence Viewer

Length: 1071 bp
ATGGAAGCTGTATTCGGGATGGCTTTAGCCACCATCATTTTGACAAGCATGGCAGCCATGGCTGCATCAGAAGCATCTCCAGCCACATTTGTGTTCGGTGACTCGTTAACAGAAGTGGGAAACAACAATTATTTGCAGTATTCTCTTGCAAAGTCAAATTTTCCTTGGTACGGGGTCGATTATGAAGGTGGTCAGGCTACCGGGAGATTCACAAATGGAAGAACTATTGGTGATTTAATATCTGCAAAGCTTGGGATTCCATCACCGCCGCCTTATCTCTCATTGTCAACGAATGATGATGCACTGCTTAATGGGGTCAACTATGCATCTGGTGGAGCAGGAATACTAAATGACACTGGACTCTATTTTATTGAGAGACTGTCCTTTGATGATCAAGTACAAAGTTTCAACAAGACAAGGGAAGTCATCAAGGCTAAGATTGGGAGTGAAGCTGCAACCAAGCTTTTAACTGAAGCTCTCTATTTCATTGGAATTGGCAGCAATGATTATGTCAATAATTTCTTACAACCCTTTTTACCTGATGGTCAAGAGTACACTCATGATGACTTTGTGGAACTGTTAATAGGCACCTTAGCGCAACAACTTAAGACTCTCTACCAGTTGGGTGCACGAAAGATGATGTTTCATGGTCTTGGTCCACTCGGCTGCATTCCTTCTCAGAGGGTTAAATCAAAGAAAGGCCAATGCTTGAAGAAAGTCAACAAGTGGGTGCTGGAATTCAACTCTAAAGTAGAGAAGCTCATTGCCTCTCTTAACAGACGCCTCCCAAAAGCAAAATTCTCATTTGCTGATACATATTCAGATGTTCTTGACTTAATCAGCAACCCTACCACATATGGTTTTAAGGTTTCCAATACGTCATGTTGCAATGTAGACACGAGCATTGGAGGGCTATGCTTACCGAATTCAAAAATATGCACGAACCGCAAAGATTATGTATTCTGGGATGCATTCCACCCCTCAGATGCAGCAAATGCAGTTCTTGCTGACAAACTCTTCTTGAGACTATTTTCTAAGCCTCCAGCCCCTGCACCAACACCTTCTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

356

Amino Acids

38.82

Weight (kDa)

7.55

Isoelectric Point (pI)

27.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 30 - 339 5.8e-40 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 587
AccI GTMKAC 1 cut(s) 894
AciI CCGC 3 cut(s) 266, 269, 946
AcsI RAATTY 4 cut(s) 157, 737, 797, 925
AcuI CTGAAG 1 cut(s) 492
AcyI GRCGYC 1 cut(s) 781
AfaI GTAC 3 cut(s) 170, 399, 554
AfiI CCNNNNNNNGG 1 cut(s) 170
AflII CTTAAG 1 cut(s) 605
AgsI TTSAA 4 cut(s) 409, 712, 742, 930
AleI CACNNNNGTG 1 cut(s) 89
AluBI AGCT 6 cut(s) 8, 250, 452, 463, 476, 760
AluI AGCT 6 cut(s) 8, 250, 452, 463, 476, 760
Alw21I GWGCWC 1 cut(s) 631
Alw26I GTCTC 2 cut(s) 370, 1018
Alw44I GTGCAC 1 cut(s) 627
AlwNI CAGNNNCTG 1 cut(s) 1049
AoxI GGCC 1 cut(s) 700
ApaLI GTGCAC 1 cut(s) 627
ApeKI GCWGC 6 cut(s) 53, 62, 452, 498, 666, 989
ApoI RAATTY 4 cut(s) 157, 737, 797, 925
AspLEI GCGC 1 cut(s) 598
AspS9I GGNCC 1 cut(s) 656
AsuC2I CCSGG 1 cut(s) 202
AsuHPI GGTGA 3 cut(s) 110, 242, 255
AvaII GGWCC 1 cut(s) 656
BaeGI GKGCMC 1 cut(s) 631
BanI GGYRCC 1 cut(s) 587
BauI CACGAG 1 cut(s) 898
Bbv12I GWGCWC 1 cut(s) 631
BbvI GCAGC 6 cut(s) 49, 65, 439, 510, 653, 1001
BccI CCATC 4 cut(s) 13, 41, 268, 536
BclI TGATCA 1 cut(s) 391
BcnI CCSGG 1 cut(s) 202
BcoDI GTCTC 2 cut(s) 370, 1018
BfrI CTTAAG 1 cut(s) 605
BisI GCNGC 7 cut(s) 54, 63, 269, 453, 499, 667, 990
BlsI GCNGC 7 cut(s) 55, 64, 270, 454, 500, 668, 991
Bme1390I CCNGG 1 cut(s) 202
Bme18I GGWCC 1 cut(s) 656
BmgT120I GGNCC 1 cut(s) 656
BmiI GGNNCC 1 cut(s) 589
BmrFI CCNGG 1 cut(s) 202
BmsI GCATC 6 cut(s) 74, 83, 289, 335, 958, 976
BpmI CTGGAG 2 cut(s) 63, 1026
Bpu10I CCTNAGC 1 cut(s) 592
BpuEI CTTGAG 1 cut(s) 1042
BpuMI CCSGG 1 cut(s) 202
BsaHI GRCGYC 1 cut(s) 781
BsaJI CCNNGG 2 cut(s) 57, 164
Bsc4I CCNNNNNNNGG 1 cut(s) 170
Bse1I ACTGG 2 cut(s) 361, 619
Bse3DI GCAATG 3 cut(s) 508, 762, 895
BseDI CCNNGG 2 cut(s) 57, 164
BseGI GGATG 2 cut(s) 24, 973
BseLI CCNNNNNNNGG 1 cut(s) 170
BseMI GCAATG 3 cut(s) 508, 762, 895
BseMII CTCAG 2 cut(s) 692, 996
BseNI ACTGG 2 cut(s) 361, 619
BseSI GKGCMC 1 cut(s) 631
BseXI GCAGC 6 cut(s) 49, 65, 439, 510, 653, 1001
BsgI GTGCAG 1 cut(s) 1035
BshFI GGCC 1 cut(s) 702
BshNI GGYRCC 1 cut(s) 587
BsiHKAI GWGCWC 1 cut(s) 631
BsiSI CCGG 1 cut(s) 201
BslI CCNNNNNNNGG 1 cut(s) 170
BsmAI GTCTC 2 cut(s) 370, 1018
BsmI GAATGC 2 cut(s) 669, 971
BsnI GGCC 1 cut(s) 702
Bsp1286I GDGCHC 1 cut(s) 631
Bsp143I GATC 1 cut(s) 391
Bsp19I CCATGG 1 cut(s) 57
BspACI CCGC 3 cut(s) 266, 269, 946
BspANI GGCC 1 cut(s) 702
BspCNI CTCAG 2 cut(s) 691, 995
BspHI TCATGA 1 cut(s) 559
BspLI GGNNCC 1 cut(s) 589
BspT107I GGYRCC 1 cut(s) 587
BspTI CTTAAG 1 cut(s) 605
BsrDI GCAATG 3 cut(s) 508, 762, 895
BsrI ACTGG 2 cut(s) 361, 619
BssECI CCNNGG 2 cut(s) 57, 164
BssMI GATC 1 cut(s) 391
BssNI GRCGYC 1 cut(s) 781
BssSI CACGAG 1 cut(s) 898
BssT1I CCWWGG 2 cut(s) 57, 164
Bst2BI CACGAG 1 cut(s) 898
Bst4CI ACNGT 2 cut(s) 381, 579
Bst6I CTCTTC 1 cut(s) 1022
BstACI GRCGYC 1 cut(s) 781
BstAFI CTTAAG 1 cut(s) 605
BstAPI GCANNNNNTGC 2 cut(s) 995, 1004
BstDEI CTNAG 5 cut(s) 435, 592, 678, 982, 1035
BstDSI CCRYGG 1 cut(s) 57
BstF5I GGATG 2 cut(s) 24, 973
BstHHI GCGC 1 cut(s) 598
BstKTI GATC 1 cut(s) 394
BstMAI GTCTC 2 cut(s) 370, 1018
BstMBI GATC 1 cut(s) 391
BstMWI GCNNNNNNNGC 7 cut(s) 59, 62, 71, 80, 945, 995, 1004
BstSCI CCNGG 1 cut(s) 200
BstSLI GKGCMC 1 cut(s) 631
BstV1I GCAGC 6 cut(s) 49, 65, 439, 510, 653, 1001
BsuRI GGCC 1 cut(s) 702
BtgI CCRYGG 1 cut(s) 57
BtsCI GGATG 2 cut(s) 24, 973
BtsI GCAGTG 1 cut(s) 302
BtsIMutI CAGTG 2 cut(s) 302, 354
CaiI CAGNNNCTG 1 cut(s) 1049
CciI TCATGA 1 cut(s) 559
CfoI GCGC 1 cut(s) 598
Cfr13I GGNCC 1 cut(s) 656
CseI GACGC 1 cut(s) 789
Csp6I GTAC 3 cut(s) 169, 398, 553
CviAII CATG 5 cut(s) 49, 58, 560, 647, 882
CviQI GTAC 3 cut(s) 169, 398, 553
DdeI CTNAG 5 cut(s) 435, 592, 678, 982, 1035
DpnI GATC 1 cut(s) 393
DpnII GATC 1 cut(s) 391
Eam1104I CTCTTC 1 cut(s) 1022
EarI CTCTTC 1 cut(s) 1022
Eco130I CCWWGG 2 cut(s) 57, 164
Eco47I GGWCC 1 cut(s) 656
Eco57I CTGAAG 1 cut(s) 492
EcoRI GAATTC 2 cut(s) 737, 925
EcoT14I CCWWGG 2 cut(s) 57, 164
EcoT22I ATGCAT 2 cut(s) 328, 973
ErhI CCWWGG 2 cut(s) 57, 164
FaeI CATG 5 cut(s) 52, 61, 563, 650, 885
FatI CATG 5 cut(s) 48, 57, 559, 646, 881
FauNDI CATATG 1 cut(s) 856
FbaI TGATCA 1 cut(s) 391
FblI GTMKAC 1 cut(s) 894
Fnu4HI GCNGC 7 cut(s) 54, 63, 269, 453, 499, 667, 990
FokI GGATG 2 cut(s) 31, 980
Fsp4HI GCNGC 7 cut(s) 54, 63, 269, 453, 499, 667, 990
GlaI GCGC 1 cut(s) 597
GluI GCNGC 7 cut(s) 54, 63, 269, 453, 499, 667, 990
GsuI CTGGAG 2 cut(s) 63, 1026
HaeIII GGCC 1 cut(s) 702
HapII CCGG 1 cut(s) 201
HgaI GACGC 1 cut(s) 789
HhaI GCGC 1 cut(s) 598
Hin1I GRCGYC 1 cut(s) 781
Hin1II CATG 5 cut(s) 52, 61, 563, 650, 885
Hin6I GCGC 1 cut(s) 596
HinP1I GCGC 1 cut(s) 596
HincII GTYRAC 4 cut(s) 108, 288, 319, 721
HindII GTYRAC 4 cut(s) 108, 288, 319, 721
HindIII AAGCTT 2 cut(s) 248, 461
HinfI GANTC 5 cut(s) 101, 207, 256, 360, 610
HpaI GTTAAC 1 cut(s) 108
HpaII CCGG 1 cut(s) 201
HphI GGTGA 3 cut(s) 110, 242, 255
Hpy166II GTNNAC 8 cut(s) 108, 288, 319, 555, 629, 659, 721, 895
Hpy188I TCNGA 4 cut(s) 70, 681, 823, 985
Hpy188III TCNNGA 5 cut(s) 16, 548, 560, 830, 1021
Hpy8I GTNNAC 8 cut(s) 108, 288, 319, 555, 629, 659, 721, 895
HpyAV CCTTC 3 cut(s) 179, 684, 1071
HpyCH4III ACNGT 2 cut(s) 381, 579
HpyCH4IV ACGT 1 cut(s) 878
HpyF10VI GCNNNNNNNGC 7 cut(s) 59, 62, 71, 80, 945, 995, 1004
HpyF3I CTNAG 5 cut(s) 435, 592, 678, 982, 1035
HpySE526I ACGT 1 cut(s) 878
Hsp92I GRCGYC 1 cut(s) 781
Hsp92II CATG 5 cut(s) 52, 61, 563, 650, 885
HspAI GCGC 1 cut(s) 596
Ksp22I TGATCA 1 cut(s) 391
KspAI GTTAAC 1 cut(s) 108
Kzo9I GATC 1 cut(s) 391
LmnI GCTCC 1 cut(s) 335
Lsp1109I GCAGC 6 cut(s) 49, 65, 439, 510, 653, 1001
LweI GCATC 6 cut(s) 74, 83, 289, 335, 958, 976
MaeII ACGT 1 cut(s) 878
MaeIII GTNAC 1 cut(s) 98
MalI GATC 1 cut(s) 393
MboI GATC 1 cut(s) 391
MboII GAAGA 3 cut(s) 231, 724, 1009
MhlI GDGCHC 1 cut(s) 631
MluCI AATT 7 cut(s) 127, 157, 492, 517, 737, 797, 925
MlyI GAGTC 3 cut(s) 95, 354, 604
MnlI CCTC 6 cut(s) 675, 778, 794, 902, 991, 1050
Mph1103I ATGCAT 2 cut(s) 328, 973
MslI CAYNNNNRTG 1 cut(s) 89
MspCI CTTAAG 1 cut(s) 605
MspI CCGG 1 cut(s) 201
MspR9I CCNGG 1 cut(s) 202
Mva1269I GAATGC 2 cut(s) 669, 971
MwoI GCNNNNNNNGC 7 cut(s) 59, 62, 71, 80, 945, 995, 1004
NciI CCSGG 1 cut(s) 202
NcoI CCATGG 1 cut(s) 57
NdeI CATATG 1 cut(s) 856
NdeII GATC 1 cut(s) 391
NlaIII CATG 5 cut(s) 52, 61, 563, 650, 885
NlaIV GGNNCC 1 cut(s) 589
NmeAIII GCCGAG 1 cut(s) 642
NmuCI GTSAC 1 cut(s) 98
NsiI ATGCAT 2 cut(s) 328, 973
OliI CACNNNNGTG 1 cut(s) 89
PagI TCATGA 1 cut(s) 559
PctI GAATGC 2 cut(s) 669, 971
PfeI GAWTC 2 cut(s) 207, 256
PkrI GCNGC 7 cut(s) 55, 64, 270, 454, 500, 668, 991
PleI GAGTC 3 cut(s) 95, 354, 604
PpsI GAGTC 3 cut(s) 95, 354, 604
PspN4I GGNNCC 1 cut(s) 589
PspPI GGNCC 1 cut(s) 656
PstNI CAGNNNCTG 1 cut(s) 1049
RsaI GTAC 3 cut(s) 170, 399, 554
RsaNI GTAC 3 cut(s) 169, 398, 553
RseI CAYNNNNRTG 1 cut(s) 89
SatI GCNGC 7 cut(s) 54, 63, 269, 453, 499, 667, 990
Sau3AI GATC 1 cut(s) 391
Sau96I GGNCC 1 cut(s) 656
SchI GAGTC 3 cut(s) 95, 354, 604
ScrFI CCNGG 1 cut(s) 202
SduI GDGCHC 1 cut(s) 631
SfaNI GCATC 6 cut(s) 74, 83, 289, 335, 958, 976
SinI GGWCC 1 cut(s) 656
SmiMI CAYNNNNRTG 1 cut(s) 89
SmlI CTYRAG 2 cut(s) 605, 1021
SmoI CTYRAG 2 cut(s) 605, 1021
Sse9I AATT 7 cut(s) 127, 157, 492, 517, 737, 797, 925
SsiI CCGC 3 cut(s) 266, 269, 946
StyD4I CCNGG 1 cut(s) 200
StyI CCWWGG 2 cut(s) 57, 164
TaaI ACNGT 2 cut(s) 381, 579
TaiI ACGT 1 cut(s) 881
TaqI TCGA 1 cut(s) 177
TasI AATT 7 cut(s) 127, 157, 492, 517, 737, 797, 925
TatI WGTACW 2 cut(s) 397, 552
TauI GCSGC 1 cut(s) 271
TfiI GAWTC 2 cut(s) 207, 256
TscAI CASTG 2 cut(s) 309, 361
TseFI GTSAC 1 cut(s) 98
TseI GCWGC 6 cut(s) 53, 62, 452, 498, 666, 989
Tsp45I GTSAC 1 cut(s) 98
TspDTI ATGAA 3 cut(s) 198, 475, 635
TspRI CASTG 2 cut(s) 309, 361
Vha464I CTTAAG 1 cut(s) 605
VneI GTGCAC 1 cut(s) 627
VpaK11BI GGWCC 1 cut(s) 656
XapI RAATTY 4 cut(s) 157, 737, 797, 925
XmiI GTMKAC 1 cut(s) 894
Zsp2I ATGCAT 2 cut(s) 328, 973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.