MD09G1274700.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0008491
Physical Location & Seq
Reverse (-)
35201321 .. 35203572
2252 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1274700.v1.1.491

Sequence Viewer

Length: 1071 bp
ATGTGGATGGCTTTAGCTACCACCATTTTCACAACCATGTTAGCCGTCGCTTCATTGGCAGCGTCTACCGTGACCTTCGTTTTCGGTGACTCCTTGACAGAAGTAGGGAACAACAACTATCTGTCGTATTCTCTTGCAAAATCAAATTATCCTTGGTATGGTGTCGATTATGCCGGTGGTCGAGCTACTGGGAGGTTCACTAATGGAAGAACCATTGGCGACATAATATCCAAAAAGCTTGGGATTCCATCACCACCACCTTATCTATCCTTGGCACAGAATGATGATGCACTGCTTAAAGGGGTTAATTATGCATCTGGTGGAGCAGGAATACTCAATGACACTGGGCTCTATTTTATTCAGAGAATGTCGTTTGATGATCAAATACAAAAATTCAACAAGACAAAGGAAGCCATCAAGGCTAAAATTGGGGCAGAGGCTGCAGCCAAGCTTTGCAGTGAAGCCATCTACTTCATTGGAATTGGCAGCAATGATTATGTCAACAATTACCTGCAACCCTTTTTAGCTGATAGTCAGCAGTACACTTACGATGAATTTGTGGAATTATTGATCGGCACCTTAGAGGGACAACTTAAGAGGCTCTACCAACTGGGTGCCCGAAAGATGATGTTCCATGGTCTCGGGCCACTTGGCTGCATTCCTTCTCAGAGGGTGAAATCAAAGACAGGCCAGTGCTTGAAAAGAGTGAACGAGTGGGTGCTAGAATTCAACTCCAAAGTACAAAAGCTCATTGCCTCTCTTAACAGGCAACTCCCACAGGCAAATCTCTCATTTGCAGACACGTATGGAGACGTCTACGACTTAATCCAAAACCCTACCGCTTATGGGTTTAAGGTTTCGAACACTTCGTGTTGCAATGTGGACACAAGCATTGGGGGGCTATGCTTGCCTAATTCAAAGCTATGCCCCAACCGGAAAGACTACGTATTCTGGGATGCATTCCACCCATCCGACGCAGCCAATGAGGTTCTCGCAGACAAACTCTTCTCGAGGCTATTTTCCGCGATTCCTCCTAGTGCACCAACACCAACACCTTCACCCTCACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

357

Amino Acids

38.94

Weight (kDa)

8.33

Isoelectric Point (pI)

27.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 26 - 335 2.1e-41 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 816
Acc36I ACCTGC 1 cut(s) 519
AccB1I GGYRCC 2 cut(s) 575, 614
AccI GTMKAC 2 cut(s) 65, 816
AccII CGCG 1 cut(s) 1025
AciI CCGC 2 cut(s) 840, 1023
AcsI RAATTY 3 cut(s) 392, 554, 725
AcyI GRCGYC 1 cut(s) 813
AdeI CACNNNGTG 1 cut(s) 870
AfaI GTAC 2 cut(s) 542, 741
AfiI CCNNNNNNNGG 2 cut(s) 158, 846
AflII CTTAAG 1 cut(s) 593
AflIII ACRYGT 1 cut(s) 801
AgsI TTSAA 4 cut(s) 397, 700, 730, 918
AluBI AGCT 7 cut(s) 17, 185, 238, 451, 527, 748, 922
AluI AGCT 7 cut(s) 17, 185, 238, 451, 527, 748, 922
Alw21I GWGCWC 1 cut(s) 1042
Alw26I GTCTC 2 cut(s) 644, 804
Alw44I GTGCAC 1 cut(s) 1038
AlwNI CAGNNNCTG 1 cut(s) 440
Ama87I CYCGRG 2 cut(s) 641, 1009
AoxI GGCC 2 cut(s) 644, 688
ApaLI GTGCAC 1 cut(s) 1038
ApeKI GCWGC 6 cut(s) 59, 440, 443, 486, 654, 977
ApoI RAATTY 3 cut(s) 392, 554, 725
AspS9I GGNCC 1 cut(s) 644
AsuHPI GGTGA 5 cut(s) 98, 243, 685, 1050, 1056
AsuII TTCGAA 1 cut(s) 860
AvaI CYCGRG 2 cut(s) 641, 1009
BaeGI GKGCMC 2 cut(s) 619, 1042
BanI GGYRCC 2 cut(s) 575, 614
BanII GRGCYC 1 cut(s) 351
Bbv12I GWGCWC 1 cut(s) 1042
BbvI GCAGC 6 cut(s) 71, 427, 455, 498, 641, 989
BccI CCATC 4 cut(s) 256, 422, 473, 976
BceAI ACGGC 1 cut(s) 29
BclI TGATCA 1 cut(s) 379
BcoDI GTCTC 2 cut(s) 644, 804
BfaI CTAG 3 cut(s) 722, 1035, 1069
BfmI CTRYAG 1 cut(s) 441
BfrI CTTAAG 1 cut(s) 593
BfuAI ACCTGC 1 cut(s) 519
BglI GCCNNNNNGGC 1 cut(s) 419
BisI GCNGC 6 cut(s) 60, 441, 444, 487, 655, 978
BlsI GCNGC 6 cut(s) 61, 442, 445, 488, 656, 979
BmeT110I CYCGRG 2 cut(s) 641, 1009
BmgT120I GGNCC 1 cut(s) 644
BmiI GGNNCC 2 cut(s) 577, 616
BmrI ACTGGG 3 cut(s) 198, 354, 620
BmsI GCATC 3 cut(s) 277, 323, 946
BmuI ACTGGG 3 cut(s) 198, 354, 620
Bpu14I TTCGAA 1 cut(s) 860
BsaAI YACGTR 2 cut(s) 804, 946
BsaHI GRCGYC 1 cut(s) 813
BsaI GGTCTC 1 cut(s) 644
BsaJI CCNNGG 3 cut(s) 152, 270, 634
BsaWI WCCGGW 1 cut(s) 933
Bsc4I CCNNNNNNNGG 2 cut(s) 158, 846
Bse118I RCCGGY 1 cut(s) 173
Bse1I ACTGG 4 cut(s) 193, 349, 615, 691
Bse3DI GCAATG 3 cut(s) 496, 750, 883
BseDI CCNNGG 3 cut(s) 152, 270, 634
BseGI GGATG 3 cut(s) 12, 961, 968
BseLI CCNNNNNNNGG 2 cut(s) 158, 846
BseMI GCAATG 3 cut(s) 496, 750, 883
BseMII CTCAG 1 cut(s) 680
BseNI ACTGG 4 cut(s) 193, 349, 615, 691
BseSI GKGCMC 2 cut(s) 619, 1042
BseXI GCAGC 6 cut(s) 71, 427, 455, 498, 641, 989
Bsh1236I CGCG 1 cut(s) 1025
BshFI GGCC 2 cut(s) 646, 690
BshNI GGYRCC 2 cut(s) 575, 614
BsiHKAI GWGCWC 1 cut(s) 1042
BsiHKCI CYCGRG 2 cut(s) 641, 1009
BsiSI CCGG 2 cut(s) 174, 934
BslFI GGGAC 1 cut(s) 600
BslI CCNNNNNNNGG 2 cut(s) 158, 846
BsmAI GTCTC 2 cut(s) 644, 804
BsmBI CGTCTC 1 cut(s) 804
BsmFI GGGAC 1 cut(s) 600
BsmI GAATGC 2 cut(s) 657, 959
BsnI GGCC 2 cut(s) 646, 690
Bso31I GGTCTC 1 cut(s) 644
BsoBI CYCGRG 2 cut(s) 641, 1009
Bsp119I TTCGAA 1 cut(s) 860
Bsp1286I GDGCHC 3 cut(s) 351, 619, 1042
Bsp143I GATC 2 cut(s) 379, 570
Bsp19I CCATGG 1 cut(s) 634
BspACI CCGC 2 cut(s) 840, 1023
BspANI GGCC 2 cut(s) 646, 690
BspCNI CTCAG 1 cut(s) 679
BspFNI CGCG 1 cut(s) 1025
BspLI GGNNCC 2 cut(s) 577, 616
BspMAI CTGCAG 1 cut(s) 445
BspMI ACCTGC 1 cut(s) 519
BspT104I TTCGAA 1 cut(s) 860
BspT107I GGYRCC 2 cut(s) 575, 614
BspTI CTTAAG 1 cut(s) 593
BspTNI GGTCTC 1 cut(s) 644
BsrDI GCAATG 3 cut(s) 496, 750, 883
BsrFI RCCGGY 1 cut(s) 173
BsrI ACTGG 4 cut(s) 193, 349, 615, 691
BssAI RCCGGY 1 cut(s) 173
BssECI CCNNGG 3 cut(s) 152, 270, 634
BssMI GATC 2 cut(s) 379, 570
BssNI GRCGYC 1 cut(s) 813
BssT1I CCWWGG 3 cut(s) 152, 270, 634
Bst4CI ACNGT 1 cut(s) 70
Bst6I CTCTTC 1 cut(s) 1010
BstACI GRCGYC 1 cut(s) 813
BstAFI CTTAAG 1 cut(s) 593
BstAPI GCANNNNNTGC 1 cut(s) 440
BstBAI YACGTR 2 cut(s) 804, 946
BstBI TTCGAA 1 cut(s) 860
BstC8I GCNNGC 1 cut(s) 908
BstDEI CTNAG 2 cut(s) 580, 666
BstDSI CCRYGG 1 cut(s) 634
BstF5I GGATG 3 cut(s) 12, 961, 968
BstFNI CGCG 1 cut(s) 1025
BstKTI GATC 2 cut(s) 382, 573
BstMAI GTCTC 2 cut(s) 644, 804
BstMBI GATC 2 cut(s) 379, 570
BstMWI GCNNNNNNNGC 4 cut(s) 56, 419, 440, 907
BstSFI CTRYAG 1 cut(s) 441
BstSLI GKGCMC 2 cut(s) 619, 1042
BstSNI TACGTA 1 cut(s) 946
BstUI CGCG 1 cut(s) 1025
BstV1I GCAGC 6 cut(s) 71, 427, 455, 498, 641, 989
BsuRI GGCC 2 cut(s) 646, 690
BtgI CCRYGG 1 cut(s) 634
BtsCI GGATG 3 cut(s) 12, 961, 968
BtsI GCAGTG 2 cut(s) 290, 463
BtsIMutI CAGTG 4 cut(s) 290, 342, 463, 698
BveI ACCTGC 1 cut(s) 519
Cac8I GCNNGC 1 cut(s) 908
CaiI CAGNNNCTG 1 cut(s) 440
Cfr10I RCCGGY 1 cut(s) 173
Cfr13I GGNCC 1 cut(s) 644
CseI GACGC 2 cut(s) 51, 983
Csp6I GTAC 2 cut(s) 541, 740
CviAII CATG 2 cut(s) 37, 635
CviQI GTAC 2 cut(s) 541, 740
DdeI CTNAG 2 cut(s) 580, 666
DpnI GATC 2 cut(s) 381, 572
DpnII GATC 2 cut(s) 379, 570
DraIII CACNNNGTG 1 cut(s) 870
Eam1104I CTCTTC 1 cut(s) 1010
EarI CTCTTC 1 cut(s) 1010
Eco105I TACGTA 1 cut(s) 946
Eco130I CCWWGG 3 cut(s) 152, 270, 634
Eco24I GRGCYC 1 cut(s) 351
Eco31I GGTCTC 1 cut(s) 644
Eco88I CYCGRG 2 cut(s) 641, 1009
EcoRI GAATTC 1 cut(s) 725
EcoT14I CCWWGG 3 cut(s) 152, 270, 634
EcoT22I ATGCAT 2 cut(s) 316, 961
EcoT38I GRGCYC 1 cut(s) 351
ErhI CCWWGG 3 cut(s) 152, 270, 634
Esp3I CGTCTC 1 cut(s) 804
FaeI CATG 2 cut(s) 40, 638
FaqI GGGAC 1 cut(s) 600
FatI CATG 2 cut(s) 36, 634
FbaI TGATCA 1 cut(s) 379
FblI GTMKAC 2 cut(s) 65, 816
Fnu4HI GCNGC 6 cut(s) 60, 441, 444, 487, 655, 978
FokI GGATG 3 cut(s) 19, 955, 968
FriOI GRGCYC 1 cut(s) 351
Fsp4HI GCNGC 6 cut(s) 60, 441, 444, 487, 655, 978
FspBI CTAG 3 cut(s) 722, 1035, 1069
GluI GCNGC 6 cut(s) 60, 441, 444, 487, 655, 978
HaeIII GGCC 2 cut(s) 646, 690
HapII CCGG 2 cut(s) 174, 934
HgaI GACGC 2 cut(s) 51, 983
Hin1I GRCGYC 1 cut(s) 813
Hin1II CATG 2 cut(s) 40, 638
HincII GTYRAC 1 cut(s) 502
HindII GTYRAC 1 cut(s) 502
HindIII AAGCTT 2 cut(s) 236, 449
HinfI GANTC 3 cut(s) 89, 244, 1027
HpaII CCGG 2 cut(s) 174, 934
HphI GGTGA 5 cut(s) 98, 243, 685, 1050, 1056
Hpy166II GTNNAC 8 cut(s) 66, 198, 502, 543, 709, 817, 883, 1040
Hpy188I TCNGA 3 cut(s) 363, 669, 973
Hpy188III TCNNGA 1 cut(s) 1009
Hpy8I GTNNAC 8 cut(s) 66, 198, 502, 543, 709, 817, 883, 1040
Hpy99I CGWCG 2 cut(s) 50, 977
HpyAV CCTTC 3 cut(s) 85, 672, 1065
HpyCH4III ACNGT 1 cut(s) 70
HpyCH4IV ACGT 3 cut(s) 803, 813, 945
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 419, 440, 907
HpyF3I CTNAG 2 cut(s) 580, 666
HpySE526I ACGT 3 cut(s) 803, 813, 945
Hsp92I GRCGYC 1 cut(s) 813
Hsp92II CATG 2 cut(s) 40, 638
Ksp22I TGATCA 1 cut(s) 379
Kzo9I GATC 2 cut(s) 379, 570
LmnI GCTCC 1 cut(s) 323
Lsp1109I GCAGC 6 cut(s) 71, 427, 455, 498, 641, 989
LweI GCATC 3 cut(s) 277, 323, 946
MaeI CTAG 3 cut(s) 722, 1035, 1069
MaeII ACGT 3 cut(s) 803, 813, 945
MaeIII GTNAC 2 cut(s) 70, 86
MalI GATC 2 cut(s) 381, 572
MboI GATC 2 cut(s) 379, 570
MboII GAAGA 2 cut(s) 219, 997
MhlI GDGCHC 3 cut(s) 351, 619, 1042
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 1 cut(s) 996
MnlI CCTC 9 cut(s) 186, 430, 577, 591, 663, 766, 979, 1005, 1041
Mph1103I ATGCAT 2 cut(s) 316, 961
MseI TTAA 6 cut(s) 297, 306, 594, 762, 824, 852
MslI CAYNNNNRTG 1 cut(s) 35
MspCI CTTAAG 1 cut(s) 593
MspI CCGG 2 cut(s) 174, 934
Mva1269I GAATGC 2 cut(s) 657, 959
MvnI CGCG 1 cut(s) 1025
MwoI GCNNNNNNNGC 4 cut(s) 56, 419, 440, 907
NcoI CCATGG 1 cut(s) 634
NdeII GATC 2 cut(s) 379, 570
NlaIII CATG 2 cut(s) 40, 638
NlaIV GGNNCC 2 cut(s) 577, 616
NmuCI GTSAC 2 cut(s) 70, 86
NsiI ATGCAT 2 cut(s) 316, 961
NspV TTCGAA 1 cut(s) 860
PaeR7I CTCGAG 1 cut(s) 1009
PcsI WCGNNNNNNNCGW 1 cut(s) 866
PctI GAATGC 2 cut(s) 657, 959
PfeI GAWTC 2 cut(s) 244, 1027
PkrI GCNGC 6 cut(s) 61, 442, 445, 488, 656, 979
PleI GAGTC 1 cut(s) 83
PpsI GAGTC 1 cut(s) 83
Ppu21I YACGTR 2 cut(s) 804, 946
PspN4I GGNNCC 2 cut(s) 577, 616
PspPI GGNCC 1 cut(s) 644
PstI CTGCAG 1 cut(s) 445
PstNI CAGNNNCTG 1 cut(s) 440
RsaI GTAC 2 cut(s) 542, 741
RsaNI GTAC 2 cut(s) 541, 740
RseI CAYNNNNRTG 1 cut(s) 35
SaqAI TTAA 6 cut(s) 297, 306, 594, 762, 824, 852
SatI GCNGC 6 cut(s) 60, 441, 444, 487, 655, 978
Sau3AI GATC 2 cut(s) 379, 570
Sau96I GGNCC 1 cut(s) 644
SchI GAGTC 1 cut(s) 83
SduI GDGCHC 3 cut(s) 351, 619, 1042
SfaNI GCATC 3 cut(s) 277, 323, 946
SfcI CTRYAG 1 cut(s) 441
Sfr274I CTCGAG 1 cut(s) 1009
SfuI TTCGAA 1 cut(s) 860
SlaI CTCGAG 1 cut(s) 1009
SmiMI CAYNNNNRTG 1 cut(s) 35
SmlI CTYRAG 2 cut(s) 593, 1009
SmoI CTYRAG 2 cut(s) 593, 1009
SnaBI TACGTA 1 cut(s) 946
SsiI CCGC 2 cut(s) 840, 1023
SspMI CTAG 3 cut(s) 722, 1035, 1069
StyI CCWWGG 3 cut(s) 152, 270, 634
TaaI ACNGT 1 cut(s) 70
TaiI ACGT 3 cut(s) 806, 816, 948
TaqI TCGA 4 cut(s) 165, 181, 860, 1010
TatI WGTACW 2 cut(s) 540, 739
TfiI GAWTC 2 cut(s) 244, 1027
Tru1I TTAA 6 cut(s) 297, 306, 594, 762, 824, 852
Tru9I TTAA 6 cut(s) 297, 306, 594, 762, 824, 852
TscAI CASTG 4 cut(s) 297, 349, 463, 698
TseFI GTSAC 2 cut(s) 70, 86
TseI GCWGC 6 cut(s) 59, 440, 443, 486, 654, 977
Tsp45I GTSAC 2 cut(s) 70, 86
TspDTI ATGAA 3 cut(s) 42, 463, 567
TspRI CASTG 4 cut(s) 297, 349, 463, 698
Vha464I CTTAAG 1 cut(s) 593
VneI GTGCAC 1 cut(s) 1038
XapI RAATTY 3 cut(s) 392, 554, 725
XhoI CTCGAG 1 cut(s) 1009
XmiI GTMKAC 2 cut(s) 65, 816
XspI CTAG 3 cut(s) 722, 1035, 1069
ZraI GACGTC 1 cut(s) 814
Zsp2I ATGCAT 2 cut(s) 316, 961
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.