Rorug03G0243500

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0008491
Physical Location & Seq
Forward (+)
22756978 .. 22759742
2765 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0243500.1

Sequence Viewer

Length: 699 bp
ATGACGACCGCAGCTCGACCCACGTGGGCTCCTGCAAAAGGTGGTAATGAACAAGGTGGTACTCGGATTTTTGGCCCATCCCAAAAGTACTCATCAAGGGACATTGCATCCCATACAACTCTAAAACCCAGAAAGGACGGGCAGGACACCGAGGAAGAACTGCAGAAGAGGAACCTCCACGAGGAGTTGGAAGACCGTGAGAGGAGGCATTTTTCATCAAAGGATAAATATAGTTATGACAGAGATCGCAGCAGGAGAGGGAGCCAGCTTTTGTTGGAAGGGACAAGAAGGGACACTGAAGACCGCATTGTACCACGTAATGTAGATGCTGATGATTCTGATTTGGAAGTCAAAAGTGATGATGACAGTGATGAAGATGATGATGATGATGAGGATGACACAGAAGCTCTTTTGGCTGAGCTTGAACAAATAAAGAGGGAAAGAGCAGAGGAGAAGCGTCGAAAGGAAGAACAACAGGTGGCGGAAGAGCTTAAAGTCAAGGAAGCAGAGCTTCTCCGAGGAAACCCATTACTTAATAATCCAATAACCGCTTCTTCCTTCAGTGTGAAAAGAAGATGGGATGATGATGTGGTGTTCAAAAATCAGGCCCGTGGTGAAACCAAGACTCCTAAGCGCTTCATCAATGACACAGTGAGGAACGACTTTCACCGCAAATTTCTGCATAAATACATGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

232

Amino Acids

27.16

Weight (kDa)

5.38

Isoelectric Point (pI)

57.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cwf_Cwc_15 PF04889 1 - 232 1.1e-76 Cwf15/Cwc15 cell cycle control protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 9, 304, 482, 549, 670
AcsI RAATTY 1 cut(s) 674
AcuI CTGAAG 2 cut(s) 318, 544
AcvI CACGTG 1 cut(s) 24
AfaI GTAC 3 cut(s) 61, 89, 312
AfeI AGCGCT 1 cut(s) 635
AfiI CCNNNNNNNGG 2 cut(s) 38, 181
AgsI TTSAA 2 cut(s) 425, 598
AjuI GAANNNNNNNTTGG 2 cut(s) 535, 567
AluBI AGCT 6 cut(s) 14, 268, 407, 421, 490, 511
AluI AGCT 6 cut(s) 14, 268, 407, 421, 490, 511
Aor51HI AGCGCT 1 cut(s) 635
AoxI GGCC 2 cut(s) 73, 606
ApeKI GCWGC 2 cut(s) 11, 249
ApoI RAATTY 1 cut(s) 674
AspLEI GCGC 1 cut(s) 636
AspS9I GGNCC 2 cut(s) 74, 607
AsuHPI GGTGA 2 cut(s) 626, 659
BanII GRGCYC 1 cut(s) 31
BauI CACGAG 1 cut(s) 179
BbrPI CACGTG 1 cut(s) 24
BbsI GAAGAC 2 cut(s) 198, 306
BbvI GCAGC 2 cut(s) 23, 261
BccI CCATC 2 cut(s) 85, 570
BfmI CTRYAG 1 cut(s) 161
BfoI RGCGCY 1 cut(s) 637
BisI GCNGC 2 cut(s) 12, 250
BlpI GCTNAGC 1 cut(s) 417
BlsI GCNGC 2 cut(s) 13, 251
BmcAI AGTACT 1 cut(s) 89
BmgT120I GGNCC 2 cut(s) 74, 607
BmiI GGNNCC 3 cut(s) 30, 173, 263
BmsI GCATC 2 cut(s) 116, 316
BpiI GAAGAC 2 cut(s) 198, 306
Bpu10I CCTNAGC 1 cut(s) 630
Bpu1102I GCTNAGC 1 cut(s) 417
BsaAI YACGTR 2 cut(s) 24, 317
BsaJI CCNNGG 3 cut(s) 150, 517, 610
BsaXI ACNNNNNCTCC 4 cut(s) 13, 43, 610, 640
Bsc4I CCNNNNNNNGG 2 cut(s) 38, 181
Bse3DI GCAATG 1 cut(s) 102
BseDI CCNNGG 3 cut(s) 150, 517, 610
BseGI GGATG 4 cut(s) 77, 107, 400, 586
BseLI CCNNNNNNNGG 2 cut(s) 38, 181
BseMI GCAATG 1 cut(s) 102
BseMII CTCAG 1 cut(s) 408
BseRI GAGGAG 3 cut(s) 197, 217, 464
BseXI GCAGC 2 cut(s) 23, 261
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 2 cut(s) 75, 608
BsiEI CGRYCG 1 cut(s) 9
BslFI GGGAC 3 cut(s) 113, 295, 305
BslI CCNNNNNNNGG 2 cut(s) 38, 181
BsmFI GGGAC 3 cut(s) 113, 295, 305
BsnI GGCC 2 cut(s) 75, 608
Bsp1286I GDGCHC 1 cut(s) 31
Bsp143I GATC 1 cut(s) 244
Bsp1720I GCTNAGC 1 cut(s) 417
BspACI CCGC 5 cut(s) 9, 304, 482, 549, 670
BspANI GGCC 2 cut(s) 75, 608
BspCNI CTCAG 1 cut(s) 409
BspLI GGNNCC 3 cut(s) 30, 173, 263
BspMAI CTGCAG 1 cut(s) 165
BspQI GCTCTTC 1 cut(s) 480
BsrDI GCAATG 1 cut(s) 102
BssECI CCNNGG 3 cut(s) 150, 517, 610
BssMI GATC 1 cut(s) 244
BssSI CACGAG 1 cut(s) 179
Bst2BI CACGAG 1 cut(s) 179
Bst4CI ACNGT 3 cut(s) 197, 368, 652
Bst6I CTCTTC 2 cut(s) 161, 480
BstBAI YACGTR 2 cut(s) 24, 317
BstC8I GCNNGC 1 cut(s) 266
BstDEI CTNAG 2 cut(s) 417, 630
BstDSI CCRYGG 1 cut(s) 610
BstENI CCTNNNNNAGG 2 cut(s) 36, 179
BstF5I GGATG 4 cut(s) 77, 107, 400, 586
BstH2I RGCGCY 1 cut(s) 637
BstHHI GCGC 1 cut(s) 636
BstKTI GATC 1 cut(s) 247
BstMBI GATC 1 cut(s) 244
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 1 cut(s) 413
BstSFI CTRYAG 1 cut(s) 161
BstV1I GCAGC 2 cut(s) 23, 261
BstV2I GAAGAC 2 cut(s) 198, 306
BsuRI GGCC 2 cut(s) 75, 608
BtgI CCRYGG 1 cut(s) 610
BtsCI GGATG 4 cut(s) 77, 107, 400, 586
BtsIMutI CAGTG 4 cut(s) 294, 373, 568, 657
Cac8I GCNNGC 1 cut(s) 266
CfoI GCGC 1 cut(s) 636
Cfr13I GGNCC 2 cut(s) 74, 607
CseI GACGC 1 cut(s) 446
Csp6I GTAC 3 cut(s) 60, 88, 311
CviAII CATG 1 cut(s) 691
CviQI GTAC 3 cut(s) 60, 88, 311
DdeI CTNAG 2 cut(s) 417, 630
DpnI GATC 1 cut(s) 246
DpnII GATC 1 cut(s) 244
Eam1104I CTCTTC 2 cut(s) 161, 480
EarI CTCTTC 2 cut(s) 161, 480
EciI GGCGGA 1 cut(s) 497
Eco24I GRGCYC 1 cut(s) 31
Eco47III AGCGCT 1 cut(s) 635
Eco57I CTGAAG 2 cut(s) 318, 544
Eco72I CACGTG 1 cut(s) 24
EcoNI CCTNNNNNAGG 2 cut(s) 36, 179
EcoT38I GRGCYC 1 cut(s) 31
FaeI CATG 1 cut(s) 694
FaiI YATR 5 cut(s) 114, 231, 237, 684, 692
FalI AAGNNNNNCTT 2 cut(s) 495, 527
FaqI GGGAC 3 cut(s) 113, 295, 305
FatI CATG 1 cut(s) 690
Fnu4HI GCNGC 2 cut(s) 12, 250
FokI GGATG 4 cut(s) 64, 94, 407, 593
FriOI GRGCYC 1 cut(s) 31
Fsp4HI GCNGC 2 cut(s) 12, 250
GlaI GCGC 1 cut(s) 635
GluI GCNGC 2 cut(s) 12, 250
HaeII RGCGCY 1 cut(s) 637
HaeIII GGCC 2 cut(s) 75, 608
HgaI GACGC 1 cut(s) 446
HhaI GCGC 1 cut(s) 636
Hin1II CATG 1 cut(s) 694
Hin6I GCGC 1 cut(s) 634
HinP1I GCGC 1 cut(s) 634
HinfI GANTC 2 cut(s) 335, 625
HphI GGTGA 2 cut(s) 626, 659
Hpy188I TCNGA 3 cut(s) 66, 340, 518
Hpy99I CGWCG 1 cut(s) 462
HpyAV CCTTC 3 cut(s) 272, 282, 568
HpyCH4III ACNGT 3 cut(s) 197, 368, 652
HpyCH4IV ACGT 2 cut(s) 23, 316
HpyCH4V TGCA 4 cut(s) 35, 107, 163, 682
HpyF10VI GCNNNNNNNGC 1 cut(s) 413
HpyF3I CTNAG 2 cut(s) 417, 630
HpySE526I ACGT 2 cut(s) 23, 316
Hsp92II CATG 1 cut(s) 694
HspAI GCGC 1 cut(s) 634
Kzo9I GATC 1 cut(s) 244
LguI GCTCTTC 1 cut(s) 480
LmnI GCTCC 2 cut(s) 34, 261
LpnPI CCDG 7 cut(s) 45, 128, 142, 238, 278, 461, 590
Lsp1109I GCAGC 2 cut(s) 23, 261
LweI GCATC 2 cut(s) 116, 316
MaeII ACGT 2 cut(s) 23, 316
MalI GATC 1 cut(s) 246
MboI GATC 1 cut(s) 244
MboII GAAGA 9 cut(s) 167, 178, 203, 311, 386, 479, 497, 546, 585
MhlI GDGCHC 1 cut(s) 31
MluCI AATT 1 cut(s) 674
MlyI GAGTC 1 cut(s) 619
MmeI TCCRAC 2 cut(s) 168, 255
MseI TTAA 2 cut(s) 492, 534
MwoI GCNNNNNNNGC 1 cut(s) 413
NdeII GATC 1 cut(s) 244
NlaIII CATG 1 cut(s) 694
NlaIV GGNNCC 3 cut(s) 30, 173, 263
PciSI GCTCTTC 1 cut(s) 480
PfeI GAWTC 1 cut(s) 335
PkrI GCNGC 2 cut(s) 13, 251
PleI GAGTC 1 cut(s) 619
PmaCI CACGTG 1 cut(s) 24
PmlI CACGTG 1 cut(s) 24
PpsI GAGTC 1 cut(s) 619
Ppu21I YACGTR 2 cut(s) 24, 317
PspCI CACGTG 1 cut(s) 24
PspN4I GGNNCC 3 cut(s) 30, 173, 263
PspPI GGNCC 2 cut(s) 74, 607
PstI CTGCAG 1 cut(s) 165
RsaI GTAC 3 cut(s) 61, 89, 312
RsaNI GTAC 3 cut(s) 60, 88, 311
SapI GCTCTTC 1 cut(s) 480
SaqAI TTAA 2 cut(s) 492, 534
SatI GCNGC 2 cut(s) 12, 250
Sau3AI GATC 1 cut(s) 244
Sau96I GGNCC 2 cut(s) 74, 607
ScaI AGTACT 1 cut(s) 89
SchI GAGTC 1 cut(s) 619
SduI GDGCHC 1 cut(s) 31
SfaNI GCATC 2 cut(s) 116, 316
SfcI CTRYAG 1 cut(s) 161
Sse9I AATT 1 cut(s) 674
SsiI CCGC 5 cut(s) 9, 304, 482, 549, 670
TaaI ACNGT 3 cut(s) 197, 368, 652
TaiI ACGT 2 cut(s) 26, 319
TaqI TCGA 2 cut(s) 16, 460
TasI AATT 1 cut(s) 674
TatI WGTACW 1 cut(s) 87
TfiI GAWTC 1 cut(s) 335
Tru1I TTAA 2 cut(s) 492, 534
Tru9I TTAA 2 cut(s) 492, 534
TscAI CASTG 4 cut(s) 301, 373, 568, 657
TseI GCWGC 2 cut(s) 11, 249
TspDTI ATGAA 4 cut(s) 63, 204, 387, 628
TspRI CASTG 4 cut(s) 301, 373, 568, 657
XagI CCTNNNNNAGG 2 cut(s) 36, 179
XapI RAATTY 1 cut(s) 674
ZrmI AGTACT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.