Rw3G025880

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0008491
Physical Location & Seq
Forward (+)
34938656 .. 34940411
1756 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G025880.1

Sequence Viewer

Length: 1056 bp
ATGGAAGCTGTATGTCGGGATGGCTTGAGCCACCATCATTTTGACAAGCATGGCAAGCCATGGCTGCATCAGAAGCATCTCCAGCCACATTTGTGTTCGGGACTCGTTACAGAAGTGGGAAACAACAATTATTTGCAGTATTCTCTTGCAAAGTCAAATTTTCCTTGGTACGGGGTCGATTATGAAGGTGGTCAGGCTACCGAGATTCACAAATGGAAGAACTATGGCTTGGGATTCCGTCACCGCCGCCTTATCTCTCATTGTCAACGAATGATGATGCACTGCTTAAGGGGGGTCAACTATGCATCTGGTGGAGCAGGAATACTAAATGACACTGGACTCTATTTTATTGAGAGACTGTCCTTTGATGATCAAGTACAAAGTTTCAACAAGACAAGGGAAGTCATCAAGGCTAAGATTGGGAGTGAAGCTGCAACCAAGCTTTTAACTGAAGCCTCCTATTTCATTGGAATTGGTATTCTTTATAATGCACAACTGGAATTCTTTTTCATGCAGCATGATTATGTCAATAATTTCTTACAACCCTTTTTACCTGATGGTCAAGAGTACACTCATGATGACTTTGTGGAACTGTTAATAGGCACCTTAGAGCAACAACTTAAGACTCTCTACCAGTTGGGTGCACGAAAGATGATGTTGCATGGTCTTGGTCCACTCGGCTGCATTCCTTCTCAGAGGGTTAAATCAAAGAAAGGCCAATGCTTGAAGAAAGTCAACAAAGAAGCTCATTGCCCTCTTAACAGACGCATCCCAAAAGCAAAATTCTCATTTGCTGATACATATTCAGATGTTCTTGACTTAATCAGCAACCCTACCACATATGGTTTTAAGGTTTCCAATACGTCATGTTGCAATGTAGACACGAGCATTGGAGGGCTATGCTTACCGAATTCAAAAATATGCACGAACCGCAAAGATTATGTAGTCTGGGATGCATTCCACCCCTCAGATGCAGCAAATGCAGTTCTTGCTGACAAACTCTTCTTGAGACTATTTTCTAAGCCTCCAGCCCCTGCACCAACACCTTCTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

351

Amino Acids

39.65

Weight (kDa)

8.84

Isoelectric Point (pI)

30.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 93 - 334 1.3e-16 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 486
AccB1I GGYRCC 1 cut(s) 602
AccI GTMKAC 1 cut(s) 879
AciI CCGC 3 cut(s) 244, 247, 931
AcsI RAATTY 4 cut(s) 157, 500, 782, 910
AcuI CTGAAG 1 cut(s) 471
AfaI GTAC 3 cut(s) 170, 378, 569
AfiI CCNNNNNNNGG 1 cut(s) 170
AflII CTTAAG 2 cut(s) 286, 620
AgsI TTSAA 3 cut(s) 388, 727, 915
AjuI GAANNNNNNNTTGG 2 cut(s) 212, 244
AleI CACNNNNGTG 1 cut(s) 91
AluBI AGCT 4 cut(s) 8, 431, 442, 746
AluI AGCT 4 cut(s) 8, 431, 442, 746
Alw21I GWGCWC 1 cut(s) 646
Alw26I GTCTC 2 cut(s) 349, 1003
Alw44I GTGCAC 1 cut(s) 642
AlwNI CAGNNNCTG 1 cut(s) 1034
AoxI GGCC 1 cut(s) 715
ApaLI GTGCAC 1 cut(s) 642
ApeKI GCWGC 5 cut(s) 64, 431, 514, 681, 974
ApoI RAATTY 4 cut(s) 157, 500, 782, 910
AspS9I GGNCC 1 cut(s) 671
AsuHPI GGTGA 1 cut(s) 233
AvaII GGWCC 1 cut(s) 671
BaeGI GKGCMC 1 cut(s) 646
BanI GGYRCC 1 cut(s) 602
BauI CACGAG 1 cut(s) 883
Bbv12I GWGCWC 1 cut(s) 646
BbvI GCAGC 5 cut(s) 51, 418, 526, 668, 986
BccI CCATC 3 cut(s) 14, 42, 551
BclI TGATCA 1 cut(s) 370
BcoDI GTCTC 2 cut(s) 349, 1003
BfrI CTTAAG 2 cut(s) 286, 620
BisI GCNGC 6 cut(s) 65, 247, 432, 515, 682, 975
BlsI GCNGC 6 cut(s) 66, 248, 433, 516, 683, 976
Bme18I GGWCC 1 cut(s) 671
BmgT120I GGNCC 1 cut(s) 671
BmiI GGNNCC 1 cut(s) 604
BmsI GCATC 7 cut(s) 76, 85, 267, 314, 777, 943, 961
BpmI CTGGAG 2 cut(s) 65, 1011
BpuEI CTTGAG 2 cut(s) 46, 1027
BsaJI CCNNGG 2 cut(s) 59, 164
Bsc4I CCNNNNNNNGG 1 cut(s) 170
Bse1I ACTGG 3 cut(s) 340, 501, 634
Bse3DI GCAATG 2 cut(s) 748, 880
BseDI CCNNGG 2 cut(s) 59, 164
BseGI GGATG 3 cut(s) 25, 768, 958
BseLI CCNNNNNNNGG 1 cut(s) 170
BseMI GCAATG 2 cut(s) 748, 880
BseMII CTCAG 2 cut(s) 707, 981
BseNI ACTGG 3 cut(s) 340, 501, 634
BseSI GKGCMC 1 cut(s) 646
BseXI GCAGC 5 cut(s) 51, 418, 526, 668, 986
BsgI GTGCAG 1 cut(s) 1020
BshFI GGCC 1 cut(s) 717
BshNI GGYRCC 1 cut(s) 602
BsiHKAI GWGCWC 1 cut(s) 646
BslFI GGGAC 1 cut(s) 114
BslI CCNNNNNNNGG 1 cut(s) 170
BsmAI GTCTC 2 cut(s) 349, 1003
BsmFI GGGAC 1 cut(s) 114
BsmI GAATGC 2 cut(s) 684, 956
BsnI GGCC 1 cut(s) 717
Bsp1286I GDGCHC 1 cut(s) 646
Bsp143I GATC 1 cut(s) 370
Bsp19I CCATGG 1 cut(s) 59
BspACI CCGC 3 cut(s) 244, 247, 931
BspANI GGCC 1 cut(s) 717
BspCNI CTCAG 2 cut(s) 706, 980
BspHI TCATGA 1 cut(s) 574
BspLI GGNNCC 1 cut(s) 604
BspT107I GGYRCC 1 cut(s) 602
BspTI CTTAAG 2 cut(s) 286, 620
BsrDI GCAATG 2 cut(s) 748, 880
BsrI ACTGG 3 cut(s) 340, 501, 634
BssECI CCNNGG 2 cut(s) 59, 164
BssMI GATC 1 cut(s) 370
BssSI CACGAG 1 cut(s) 883
BssT1I CCWWGG 2 cut(s) 59, 164
Bst2BI CACGAG 1 cut(s) 883
Bst4CI ACNGT 2 cut(s) 360, 594
Bst6I CTCTTC 1 cut(s) 1007
BstAFI CTTAAG 2 cut(s) 286, 620
BstAPI GCANNNNNTGC 2 cut(s) 980, 989
BstC8I GCNNGC 1 cut(s) 56
BstDEI CTNAG 5 cut(s) 414, 607, 693, 967, 1020
BstDSI CCRYGG 1 cut(s) 59
BstF5I GGATG 3 cut(s) 25, 768, 958
BstKTI GATC 1 cut(s) 373
BstMAI GTCTC 2 cut(s) 349, 1003
BstMBI GATC 1 cut(s) 370
BstMWI GCNNNNNNNGC 7 cut(s) 55, 64, 73, 82, 930, 980, 989
BstSLI GKGCMC 1 cut(s) 646
BstV1I GCAGC 5 cut(s) 51, 418, 526, 668, 986
BsuRI GGCC 1 cut(s) 717
BtgI CCRYGG 1 cut(s) 59
BtsCI GGATG 3 cut(s) 25, 768, 958
BtsI GCAGTG 1 cut(s) 280
BtsIMutI CAGTG 2 cut(s) 280, 333
Cac8I GCNNGC 1 cut(s) 56
CaiI CAGNNNCTG 1 cut(s) 1034
CciI TCATGA 1 cut(s) 574
Cfr13I GGNCC 1 cut(s) 671
CseI GACGC 1 cut(s) 774
Csp6I GTAC 3 cut(s) 169, 377, 568
CviAII CATG 7 cut(s) 50, 60, 511, 518, 575, 662, 867
CviQI GTAC 3 cut(s) 169, 377, 568
DdeI CTNAG 5 cut(s) 414, 607, 693, 967, 1020
DpnI GATC 1 cut(s) 372
DpnII GATC 1 cut(s) 370
Eam1104I CTCTTC 1 cut(s) 1007
EarI CTCTTC 1 cut(s) 1007
Eco130I CCWWGG 2 cut(s) 59, 164
Eco47I GGWCC 1 cut(s) 671
Eco57I CTGAAG 1 cut(s) 471
EcoRI GAATTC 2 cut(s) 500, 910
EcoT14I CCWWGG 2 cut(s) 59, 164
EcoT22I ATGCAT 2 cut(s) 307, 958
ErhI CCWWGG 2 cut(s) 59, 164
FaeI CATG 7 cut(s) 53, 63, 514, 521, 578, 665, 870
FaqI GGGAC 1 cut(s) 114
FatI CATG 7 cut(s) 49, 59, 510, 517, 574, 661, 866
FauNDI CATATG 1 cut(s) 841
FbaI TGATCA 1 cut(s) 370
FblI GTMKAC 1 cut(s) 879
Fnu4HI GCNGC 6 cut(s) 65, 247, 432, 515, 682, 975
FokI GGATG 3 cut(s) 32, 755, 965
Fsp4HI GCNGC 6 cut(s) 65, 247, 432, 515, 682, 975
GluI GCNGC 6 cut(s) 65, 247, 432, 515, 682, 975
GsuI CTGGAG 2 cut(s) 65, 1011
HaeIII GGCC 1 cut(s) 717
HgaI GACGC 1 cut(s) 774
Hin1II CATG 7 cut(s) 53, 63, 514, 521, 578, 665, 870
HincII GTYRAC 3 cut(s) 266, 298, 736
HindII GTYRAC 3 cut(s) 266, 298, 736
HindIII AAGCTT 1 cut(s) 440
HinfI GANTC 5 cut(s) 102, 205, 234, 339, 625
HphI GGTGA 1 cut(s) 233
Hpy166II GTNNAC 7 cut(s) 266, 298, 570, 644, 674, 736, 880
Hpy188I TCNGA 4 cut(s) 72, 696, 808, 970
Hpy188III TCNNGA 6 cut(s) 17, 99, 563, 575, 815, 1006
Hpy8I GTNNAC 7 cut(s) 266, 298, 570, 644, 674, 736, 880
HpyAV CCTTC 3 cut(s) 179, 699, 1056
HpyCH4III ACNGT 2 cut(s) 360, 594
HpyCH4IV ACGT 1 cut(s) 863
HpyF10VI GCNNNNNNNGC 7 cut(s) 55, 64, 73, 82, 930, 980, 989
HpyF3I CTNAG 5 cut(s) 414, 607, 693, 967, 1020
HpySE526I ACGT 1 cut(s) 863
Hsp92II CATG 7 cut(s) 53, 63, 514, 521, 578, 665, 870
Ksp22I TGATCA 1 cut(s) 370
Kzo9I GATC 1 cut(s) 370
LmnI GCTCC 1 cut(s) 314
Lsp1109I GCAGC 5 cut(s) 51, 418, 526, 668, 986
LweI GCATC 7 cut(s) 76, 85, 267, 314, 777, 943, 961
MaeII ACGT 1 cut(s) 863
MaeIII GTNAC 2 cut(s) 106, 239
MalI GATC 1 cut(s) 372
MboI GATC 1 cut(s) 370
MboII GAAGA 3 cut(s) 229, 739, 994
MhlI GDGCHC 1 cut(s) 646
MluCI AATT 7 cut(s) 127, 157, 471, 500, 532, 782, 910
MlyI GAGTC 3 cut(s) 96, 333, 619
MnlI CCTC 6 cut(s) 466, 690, 765, 887, 976, 1035
Mph1103I ATGCAT 2 cut(s) 307, 958
MseI TTAA 8 cut(s) 287, 446, 596, 621, 702, 759, 821, 849
MslI CAYNNNNRTG 2 cut(s) 91, 522
MspCI CTTAAG 2 cut(s) 286, 620
Mva1269I GAATGC 2 cut(s) 684, 956
MwoI GCNNNNNNNGC 7 cut(s) 55, 64, 73, 82, 930, 980, 989
NcoI CCATGG 1 cut(s) 59
NdeI CATATG 1 cut(s) 841
NdeII GATC 1 cut(s) 370
NlaIII CATG 7 cut(s) 53, 63, 514, 521, 578, 665, 870
NlaIV GGNNCC 1 cut(s) 604
NmeAIII GCCGAG 1 cut(s) 657
NmuCI GTSAC 1 cut(s) 239
NsiI ATGCAT 2 cut(s) 307, 958
OliI CACNNNNGTG 1 cut(s) 91
PagI TCATGA 1 cut(s) 574
PctI GAATGC 2 cut(s) 684, 956
PfeI GAWTC 2 cut(s) 205, 234
PkrI GCNGC 6 cut(s) 66, 248, 433, 516, 683, 976
PleI GAGTC 3 cut(s) 96, 333, 619
PpsI GAGTC 3 cut(s) 96, 333, 619
PsiI TTATAA 1 cut(s) 486
PspN4I GGNNCC 1 cut(s) 604
PspPI GGNCC 1 cut(s) 671
PstNI CAGNNNCTG 1 cut(s) 1034
RsaI GTAC 3 cut(s) 170, 378, 569
RsaNI GTAC 3 cut(s) 169, 377, 568
RseI CAYNNNNRTG 2 cut(s) 91, 522
SaqAI TTAA 8 cut(s) 287, 446, 596, 621, 702, 759, 821, 849
SatI GCNGC 6 cut(s) 65, 247, 432, 515, 682, 975
Sau3AI GATC 1 cut(s) 370
Sau96I GGNCC 1 cut(s) 671
SchI GAGTC 3 cut(s) 96, 333, 619
SduI GDGCHC 1 cut(s) 646
SfaNI GCATC 7 cut(s) 76, 85, 267, 314, 777, 943, 961
SinI GGWCC 1 cut(s) 671
SmiMI CAYNNNNRTG 2 cut(s) 91, 522
SmlI CTYRAG 4 cut(s) 25, 286, 620, 1006
SmoI CTYRAG 4 cut(s) 25, 286, 620, 1006
Sse9I AATT 7 cut(s) 127, 157, 471, 500, 532, 782, 910
SsiI CCGC 3 cut(s) 244, 247, 931
StyI CCWWGG 2 cut(s) 59, 164
TaaI ACNGT 2 cut(s) 360, 594
TaiI ACGT 1 cut(s) 866
TaqI TCGA 1 cut(s) 177
TasI AATT 7 cut(s) 127, 157, 471, 500, 532, 782, 910
TatI WGTACW 2 cut(s) 376, 567
TauI GCSGC 1 cut(s) 249
TfiI GAWTC 2 cut(s) 205, 234
Tru1I TTAA 8 cut(s) 287, 446, 596, 621, 702, 759, 821, 849
Tru9I TTAA 8 cut(s) 287, 446, 596, 621, 702, 759, 821, 849
TscAI CASTG 2 cut(s) 287, 340
TseFI GTSAC 1 cut(s) 239
TseI GCWGC 5 cut(s) 64, 431, 514, 681, 974
Tsp45I GTSAC 1 cut(s) 239
TspDTI ATGAA 3 cut(s) 198, 454, 499
TspGWI ACGGA 1 cut(s) 227
TspRI CASTG 2 cut(s) 287, 340
Vha464I CTTAAG 2 cut(s) 286, 620
VneI GTGCAC 1 cut(s) 642
VpaK11BI GGWCC 1 cut(s) 671
XapI RAATTY 4 cut(s) 157, 500, 782, 910
XmiI GTMKAC 1 cut(s) 879
Zsp2I ATGCAT 2 cut(s) 307, 958
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.