MD00G1116600.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0008491
Physical Location & Seq
Reverse (-)
24664315 .. 24666587
2273 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1116600.v1.1.491

Sequence Viewer

Length: 1053 bp
ATGGAAGTGACAATGTGGATGGCTCTAGCTACCACCATTTTCGCAACCATGGTAGCCGTGCCTTCATTGGCAGCGACTACCGTGACCTTCACCTTCGGTGACTCCTTGACAGATGTAGGAAACAACAACTATCTGCAGTATTCTCTTGCAAAGTCAAATTATCCTTGGTATGGTATCGATTATGTTGGTGGTCGACCTACTGGGAGGTTCAGTAATGGAAGAACCATCGGTGACGTAATATCCCAAAAGATGGGGATCATTCCATCGCCACCACCTTATCTATCCTTGTCACCGAACGATGATCAACTGCTTAAAGGGGTTAATTATGCATCTGGTGGAGCAGGAATACTCAATAGCACTGGACTCTATTTTATTCAGAGGCTGAGTCTTGATGATCAAATACAAGATTTCAAAAAGACAACGGATGTCATCAAGGCTAAGATTGGGGCACAGGCTGCAGCCAAGCTTTGCAAGGATGCCGTCTACTTCATTGGACTTGGGAGCAATGATTATATCAACAATTACCTGCAACCCTTTTTACCTACTGGTCGGCAGTACACTCCCGAACAATTTGCGGAATTATTGGTCGGTACCTTAGAGAGACAATTTAAGAGGCTCTACCAGCTGGGTGCCCGAAATATGACGTTCCATGGTCTCGGACCACTTGGCTGCATTCCTTCTCAAAGAGTCAGATCAAACACAGGGCAGTGCTTAAACCAAGTGAACCAGATGATGCTAGGATTCAACTCCAAAGTACAAAAGCTCCTTGCCTCTCTTAACAGCCAACTCCCAGGGGCAAAACTCTCATTTGCAGACACATACGGAGACGTCCTCGACTTAATCCAAAACCCTACAGCTTCCGGGTTTAAGGTTTCGAACCTTCCGTGTTGCAATGTGGCCACAAACATTGGAGGGCTATGCTTGCCTAACTCACAGGTATGCAGCAACCGGAGAGACTACGTGTTCTGGGACGCATTCCACCCTACCGACGCAGCGAACGCGGTTCTCGCAGATAAACTCTACCCGAAACTATTCGCGCCTCCTTCACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

351

Amino Acids

38.12

Weight (kDa)

8.72

Isoelectric Point (pI)

27.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 30 - 340 2.6e-36 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 831
Acc36I ACCTGC 1 cut(s) 534
Acc65I GGTACC 1 cut(s) 590
AccB1I GGYRCC 2 cut(s) 590, 629
AccB7I CCANNNNNTGG 1 cut(s) 250
AccI GTMKAC 2 cut(s) 193, 483
AccII CGCG 2 cut(s) 1001, 1037
AciI CCGC 2 cut(s) 575, 1001
AclWI GGATC 1 cut(s) 263
AcoI YGGCCR 1 cut(s) 897
AcyI GRCGYC 1 cut(s) 828
AfaI GTAC 3 cut(s) 557, 592, 756
AfiI CCNNNNNNNGG 2 cut(s) 170, 250
AflIII ACRYGT 1 cut(s) 960
AgsI TTSAA 2 cut(s) 412, 745
AjnI CCWGG 1 cut(s) 790
AluBI AGCT 5 cut(s) 29, 466, 625, 763, 857
AluI AGCT 5 cut(s) 29, 466, 625, 763, 857
Alw26I GTCTC 4 cut(s) 595, 659, 819, 948
AlwI GGATC 1 cut(s) 263
AlwNI CAGNNNCTG 1 cut(s) 382
AoxI GGCC 1 cut(s) 897
ApeKI GCWGC 6 cut(s) 71, 455, 458, 669, 942, 992
Asp700I GAANNNNTTC 1 cut(s) 1031
Asp718I GGTACC 1 cut(s) 590
AspLEI GCGC 1 cut(s) 1039
AspS9I GGNCC 1 cut(s) 659
AsuC2I CCSGG 1 cut(s) 862
AsuHPI GGTGA 5 cut(s) 82, 110, 242, 282, 1038
AsuII TTCGAA 1 cut(s) 875
AvaII GGWCC 1 cut(s) 659
BaeGI GKGCMC 2 cut(s) 451, 634
BalI TGGCCA 1 cut(s) 899
BanI GGYRCC 2 cut(s) 590, 629
BbvI GCAGC 6 cut(s) 83, 442, 470, 656, 954, 1004
BccI CCATC 4 cut(s) 13, 233, 244, 271
BceAI ACGGC 2 cut(s) 41, 464
BcgI CGANNNNNNTGC 2 cut(s) 554, 588
BciT130I CCWGG 1 cut(s) 792
BclI TGATCA 2 cut(s) 301, 394
BcnI CCSGG 1 cut(s) 862
BcoDI GTCTC 4 cut(s) 595, 659, 819, 948
BfaI CTAG 3 cut(s) 26, 737, 1051
BfmI CTRYAG 3 cut(s) 134, 456, 852
BfuAI ACCTGC 1 cut(s) 534
BisI GCNGC 6 cut(s) 72, 456, 459, 670, 943, 993
BlsI GCNGC 6 cut(s) 73, 457, 460, 671, 944, 994
Bme1390I CCNGG 2 cut(s) 792, 862
Bme18I GGWCC 1 cut(s) 659
BmgT120I GGNCC 1 cut(s) 659
BmiI GGNNCC 2 cut(s) 592, 631
BmrFI CCNGG 2 cut(s) 792, 862
BmrI ACTGGG 1 cut(s) 210
BmsI GCATC 3 cut(s) 338, 466, 723
BmuI ACTGGG 1 cut(s) 210
BplI GAGNNNNNCTC 2 cut(s) 816, 848
Bpu14I TTCGAA 1 cut(s) 875
BpuMI CCSGG 1 cut(s) 862
Bsa29I ATCGAT 1 cut(s) 177
BsaAI YACGTR 1 cut(s) 961
BsaBI GATNNNNATC 1 cut(s) 254
BsaHI GRCGYC 1 cut(s) 828
BsaI GGTCTC 1 cut(s) 659
BsaJI CCNNGG 5 cut(s) 48, 164, 649, 790, 791
BsaWI WCCGGW 1 cut(s) 948
BsaXI ACNNNNNCTCC 4 cut(s) 196, 226, 747, 777
Bsc4I CCNNNNNNNGG 2 cut(s) 170, 250
Bse1I ACTGG 3 cut(s) 205, 364, 550
Bse3DI GCAATG 2 cut(s) 511, 898
Bse8I GATNNNNATC 1 cut(s) 254
BseBI CCWGG 1 cut(s) 792
BseCI ATCGAT 1 cut(s) 177
BseDI CCNNGG 5 cut(s) 48, 164, 649, 790, 791
BseGI GGATG 3 cut(s) 24, 430, 481
BseJI GATNNNNATC 1 cut(s) 254
BseLI CCNNNNNNNGG 2 cut(s) 170, 250
BseMI GCAATG 2 cut(s) 511, 898
BseMII CTCAG 1 cut(s) 374
BseNI ACTGG 3 cut(s) 205, 364, 550
BseSI GKGCMC 2 cut(s) 451, 634
BseXI GCAGC 6 cut(s) 83, 442, 470, 656, 954, 1004
BseYI CCCAGC 1 cut(s) 625
Bsh1236I CGCG 2 cut(s) 1001, 1037
BshFI GGCC 1 cut(s) 899
BshNI GGYRCC 2 cut(s) 590, 629
BshVI ATCGAT 1 cut(s) 177
BsiSI CCGG 2 cut(s) 861, 949
BslFI GGGAC 1 cut(s) 983
BslI CCNNNNNNNGG 2 cut(s) 170, 250
BsmAI GTCTC 4 cut(s) 595, 659, 819, 948
BsmBI CGTCTC 1 cut(s) 819
BsmFI GGGAC 1 cut(s) 983
BsmI GAATGC 2 cut(s) 672, 974
BsnI GGCC 1 cut(s) 899
Bso31I GGTCTC 1 cut(s) 659
Bsp119I TTCGAA 1 cut(s) 875
Bsp1286I GDGCHC 2 cut(s) 451, 634
Bsp143I GATC 4 cut(s) 255, 301, 394, 692
Bsp19I CCATGG 2 cut(s) 48, 649
BspACI CCGC 2 cut(s) 575, 1001
BspANI GGCC 1 cut(s) 899
BspCNI CTCAG 1 cut(s) 375
BspDI ATCGAT 1 cut(s) 177
BspFNI CGCG 2 cut(s) 1001, 1037
BspLI GGNNCC 2 cut(s) 592, 631
BspMAI CTGCAG 2 cut(s) 138, 460
BspMI ACCTGC 1 cut(s) 534
BspPI GGATC 1 cut(s) 263
BspT104I TTCGAA 1 cut(s) 875
BspT107I GGYRCC 2 cut(s) 590, 629
BspTNI GGTCTC 1 cut(s) 659
BsrDI GCAATG 2 cut(s) 511, 898
BsrI ACTGG 3 cut(s) 205, 364, 550
BssECI CCNNGG 5 cut(s) 48, 164, 649, 790, 791
BssMI GATC 4 cut(s) 255, 301, 394, 692
BssNI GRCGYC 1 cut(s) 828
BssT1I CCWWGG 3 cut(s) 48, 164, 649
Bst2UI CCWGG 1 cut(s) 792
Bst4CI ACNGT 1 cut(s) 82
BstACI GRCGYC 1 cut(s) 828
BstAPI GCANNNNNTGC 1 cut(s) 455
BstBAI YACGTR 1 cut(s) 961
BstBI TTCGAA 1 cut(s) 875
BstC8I GCNNGC 1 cut(s) 923
BstDEI CTNAG 3 cut(s) 383, 438, 595
BstDSI CCRYGG 2 cut(s) 48, 649
BstF5I GGATG 3 cut(s) 24, 430, 481
BstFNI CGCG 2 cut(s) 1001, 1037
BstHHI GCGC 1 cut(s) 1039
BstKTI GATC 4 cut(s) 258, 304, 397, 695
BstMAI GTCTC 4 cut(s) 595, 659, 819, 948
BstMBI GATC 4 cut(s) 255, 301, 394, 692
BstMWI GCNNNNNNNGC 5 cut(s) 455, 622, 922, 998, 1007
BstNI CCWGG 1 cut(s) 792
BstSCI CCNGG 2 cut(s) 790, 860
BstSFI CTRYAG 3 cut(s) 134, 456, 852
BstSLI GKGCMC 2 cut(s) 451, 634
BstUI CGCG 2 cut(s) 1001, 1037
BstV1I GCAGC 6 cut(s) 83, 442, 470, 656, 954, 1004
Bsu15I ATCGAT 1 cut(s) 177
BsuRI GGCC 1 cut(s) 899
BsuTUI ATCGAT 1 cut(s) 177
BtgI CCRYGG 2 cut(s) 48, 649
BtgZI GCGATG 1 cut(s) 249
BtsCI GGATG 3 cut(s) 24, 430, 481
BtsI GCAGTG 1 cut(s) 713
BtsIMutI CAGTG 2 cut(s) 357, 713
BveI ACCTGC 1 cut(s) 534
Cac8I GCNNGC 1 cut(s) 923
CaiI CAGNNNCTG 1 cut(s) 382
CfoI GCGC 1 cut(s) 1039
Cfr13I GGNCC 1 cut(s) 659
ClaI ATCGAT 1 cut(s) 177
CseI GACGC 2 cut(s) 980, 998
Csp6I GTAC 3 cut(s) 556, 591, 755
CspCI CAANNNNNGTGG 2 cut(s) 889, 924
CviAII CATG 2 cut(s) 49, 650
CviQI GTAC 3 cut(s) 556, 591, 755
DdeI CTNAG 3 cut(s) 383, 438, 595
DpnI GATC 4 cut(s) 257, 303, 396, 694
DpnII GATC 4 cut(s) 255, 301, 394, 692
EaeI YGGCCR 1 cut(s) 897
Eco130I CCWWGG 3 cut(s) 48, 164, 649
Eco31I GGTCTC 1 cut(s) 659
Eco47I GGWCC 1 cut(s) 659
EcoRII CCWGG 1 cut(s) 790
EcoT14I CCWWGG 3 cut(s) 48, 164, 649
EcoT22I ATGCAT 1 cut(s) 331
ErhI CCWWGG 3 cut(s) 48, 164, 649
Esp3I CGTCTC 1 cut(s) 819
FaeI CATG 2 cut(s) 52, 653
FaqI GGGAC 1 cut(s) 983
FatI CATG 2 cut(s) 48, 649
FbaI TGATCA 2 cut(s) 301, 394
FblI GTMKAC 2 cut(s) 193, 483
Fnu4HI GCNGC 6 cut(s) 72, 456, 459, 670, 943, 993
FokI GGATG 3 cut(s) 31, 437, 488
Fsp4HI GCNGC 6 cut(s) 72, 456, 459, 670, 943, 993
FspBI CTAG 3 cut(s) 26, 737, 1051
GlaI GCGC 1 cut(s) 1038
GluI GCNGC 6 cut(s) 72, 456, 459, 670, 943, 993
GsaI CCCAGC 1 cut(s) 629
HaeIII GGCC 1 cut(s) 899
HapII CCGG 2 cut(s) 861, 949
HgaI GACGC 2 cut(s) 980, 998
HhaI GCGC 1 cut(s) 1039
Hin1I GRCGYC 1 cut(s) 828
Hin1II CATG 2 cut(s) 52, 653
Hin6I GCGC 1 cut(s) 1037
HinP1I GCGC 1 cut(s) 1037
HincII GTYRAC 1 cut(s) 194
HindII GTYRAC 1 cut(s) 194
HindIII AAGCTT 1 cut(s) 464
HinfI GANTC 5 cut(s) 101, 363, 385, 687, 741
HpaII CCGG 2 cut(s) 861, 949
HphI GGTGA 5 cut(s) 82, 110, 242, 282, 1038
Hpy166II GTNNAC 4 cut(s) 194, 484, 558, 724
Hpy188I TCNGA 3 cut(s) 378, 659, 692
Hpy188III TCNNGA 2 cut(s) 389, 563
Hpy8I GTNNAC 4 cut(s) 194, 484, 558, 724
Hpy99I CGWCG 1 cut(s) 992
HpyAV CCTTC 6 cut(s) 72, 97, 103, 687, 890, 1053
HpyCH4III ACNGT 1 cut(s) 82
HpyCH4IV ACGT 4 cut(s) 234, 644, 828, 960
HpyF10VI GCNNNNNNNGC 5 cut(s) 455, 622, 922, 998, 1007
HpyF3I CTNAG 3 cut(s) 383, 438, 595
HpySE526I ACGT 4 cut(s) 234, 644, 828, 960
Hsp92I GRCGYC 1 cut(s) 828
Hsp92II CATG 2 cut(s) 52, 653
HspAI GCGC 1 cut(s) 1037
KpnI GGTACC 1 cut(s) 594
Ksp22I TGATCA 2 cut(s) 301, 394
Kzo9I GATC 4 cut(s) 255, 301, 394, 692
LmnI GCTCC 3 cut(s) 338, 501, 768
Lsp1109I GCAGC 6 cut(s) 83, 442, 470, 656, 954, 1004
LweI GCATC 3 cut(s) 338, 466, 723
MaeI CTAG 3 cut(s) 26, 737, 1051
MaeII ACGT 4 cut(s) 234, 644, 828, 960
MaeIII GTNAC 5 cut(s) 7, 82, 98, 230, 288
MalI GATC 4 cut(s) 257, 303, 396, 694
MboI GATC 4 cut(s) 255, 301, 394, 692
MboII GAAGA 1 cut(s) 231
MhlI GDGCHC 2 cut(s) 451, 634
MlsI TGGCCA 1 cut(s) 899
MluCI AATT 6 cut(s) 157, 322, 520, 569, 578, 605
MluNI TGGCCA 1 cut(s) 899
MlyI GAGTC 4 cut(s) 95, 357, 394, 696
MnlI CCTC 7 cut(s) 198, 372, 606, 781, 842, 905, 1050
Mox20I TGGCCA 1 cut(s) 899
Mph1103I ATGCAT 1 cut(s) 331
MroXI GAANNNNTTC 1 cut(s) 1031
MscI TGGCCA 1 cut(s) 899
MseI TTAA 7 cut(s) 312, 321, 609, 713, 777, 839, 867
MslI CAYNNNNRTG 1 cut(s) 937
Msp20I TGGCCA 1 cut(s) 899
MspA1I CMGCKG 1 cut(s) 625
MspI CCGG 2 cut(s) 861, 949
MspR9I CCNGG 2 cut(s) 792, 862
Mva1269I GAATGC 2 cut(s) 672, 974
MvaI CCWGG 1 cut(s) 792
MvnI CGCG 2 cut(s) 1001, 1037
MwoI GCNNNNNNNGC 5 cut(s) 455, 622, 922, 998, 1007
NciI CCSGG 1 cut(s) 862
NcoI CCATGG 2 cut(s) 48, 649
NdeII GATC 4 cut(s) 255, 301, 394, 692
NlaIII CATG 2 cut(s) 52, 653
NlaIV GGNNCC 2 cut(s) 592, 631
NmuCI GTSAC 5 cut(s) 7, 82, 98, 230, 288
NsiI ATGCAT 1 cut(s) 331
NspV TTCGAA 1 cut(s) 875
PasI CCCWGGG 1 cut(s) 791
PcsI WCGNNNNNNNCGW 1 cut(s) 881
PctI GAATGC 2 cut(s) 672, 974
PdmI GAANNNNTTC 1 cut(s) 1031
PfeI GAWTC 1 cut(s) 741
PflMI CCANNNNNTGG 1 cut(s) 250
PkrI GCNGC 6 cut(s) 73, 457, 460, 671, 944, 994
PleI GAGTC 4 cut(s) 95, 357, 393, 695
PpsI GAGTC 4 cut(s) 95, 357, 393, 695
Ppu21I YACGTR 1 cut(s) 961
Psp6I CCWGG 1 cut(s) 790
PspFI CCCAGC 1 cut(s) 625
PspGI CCWGG 1 cut(s) 790
PspN4I GGNNCC 2 cut(s) 592, 631
PspPI GGNCC 1 cut(s) 659
PstI CTGCAG 2 cut(s) 138, 460
PstNI CAGNNNCTG 1 cut(s) 382
PvuII CAGCTG 1 cut(s) 625
RsaI GTAC 3 cut(s) 557, 592, 756
RsaNI GTAC 3 cut(s) 556, 591, 755
RseI CAYNNNNRTG 1 cut(s) 937
SalI GTCGAC 1 cut(s) 192
SaqAI TTAA 7 cut(s) 312, 321, 609, 713, 777, 839, 867
SatI GCNGC 6 cut(s) 72, 456, 459, 670, 943, 993
Sau3AI GATC 4 cut(s) 255, 301, 394, 692
Sau96I GGNCC 1 cut(s) 659
SchI GAGTC 4 cut(s) 95, 357, 394, 696
ScrFI CCNGG 2 cut(s) 792, 862
SduI GDGCHC 2 cut(s) 451, 634
SfaNI GCATC 3 cut(s) 338, 466, 723
SfcI CTRYAG 3 cut(s) 134, 456, 852
SfuI TTCGAA 1 cut(s) 875
SinI GGWCC 1 cut(s) 659
SmiMI CAYNNNNRTG 1 cut(s) 937
Sse9I AATT 6 cut(s) 157, 322, 520, 569, 578, 605
SsiI CCGC 2 cut(s) 575, 1001
SspMI CTAG 3 cut(s) 26, 737, 1051
StyD4I CCNGG 2 cut(s) 790, 860
StyI CCWWGG 3 cut(s) 48, 164, 649
TaaI ACNGT 1 cut(s) 82
TaiI ACGT 4 cut(s) 237, 647, 831, 963
TaqI TCGA 4 cut(s) 177, 193, 834, 875
TasI AATT 6 cut(s) 157, 322, 520, 569, 578, 605
TatI WGTACW 2 cut(s) 555, 754
TfiI GAWTC 1 cut(s) 741
Tru1I TTAA 7 cut(s) 312, 321, 609, 713, 777, 839, 867
Tru9I TTAA 7 cut(s) 312, 321, 609, 713, 777, 839, 867
TscAI CASTG 2 cut(s) 364, 713
TseFI GTSAC 5 cut(s) 7, 82, 98, 230, 288
TseI GCWGC 6 cut(s) 71, 455, 458, 669, 942, 992
Tsp45I GTSAC 5 cut(s) 7, 82, 98, 230, 288
TspDTI ATGAA 2 cut(s) 54, 478
TspGWI ACGGA 3 cut(s) 437, 837, 873
TspRI CASTG 2 cut(s) 364, 713
Van91I CCANNNNNTGG 1 cut(s) 250
VpaK11BI GGWCC 1 cut(s) 659
XmiI GTMKAC 2 cut(s) 193, 483
XmnI GAANNNNTTC 1 cut(s) 1031
XspI CTAG 3 cut(s) 26, 737, 1051
ZraI GACGTC 1 cut(s) 829
Zsp2I ATGCAT 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.