Rh3AG291900

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0008491
Physical Location & Seq
Forward (+)
35312708 .. 35314993
2286 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG291900.1

Sequence Viewer

Length: 873 bp
ATGGAAGCTGTATTCGGGATGGCTCTAGCCACCATCATTTTGACAAGCATGGCAGCCATGGCTGCATCAGAAGCATCTCCAGCCACATTTGTGTTCGGTGACTCGTTAACAGAAGTGGGAAACAACAATTATTTGCAGTATTCTCTTGCAAAGTCAAATTTTCCTTGGTACGGGGTCGATTATGAAGGTGGTCAGGCTACCGGGAGATTCACAAATGGAAGAACTATTGGTGATTTAATATCTGCAAAGCTTGGGATTCCATCACCGCCGCCTTATCTCTCATTGTCAACGAATGATGATGCACTGCTTAAGGGGGTCAACTATGCATCTGGTGGTGCAGGAATACTAAATGACACTGGACTCTATTTTATTGAGAGACTGTCCTTTGATGATCAAGTACAAAGTTTCAACAAGACAAGGGAAGTCATCAAGGCTAAGATTGGGAGTGAAGCTGCAACCAAGCTTTTAACTGAAGCTCTCTATTTCATTGGAATTGGCAGCAATGATTATGTCAATAATTTCTTACAACCCTTTTTACCTGATGGTCAAGAGTACACTCATGATGACTTTGTGGAACTGTTAATAGGCACCTTAGAGCAACAACTTAAGACTCTTTACCAGTTGGGTGCACGAAAGATGATGTTTCATGGTCTTGGTCCACTCGGCTGCATTCCTTCTCAGAGGGTTAAATCAAAGAAAGGCCAATGCTTGAAGAAAGTCAACAAGTGGGTGCTGGAATTCAACTCTAAAGTAGAGAAGCTCATTGCCTCTCTTAACAGACGCCTCCCAAAAGCAAAATTCTCATTTGCTGATACATATTCAGATGTTCTTGACTTAATCAGCAACCCTACCACATATGGTATTAATCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

290

Amino Acids

31.75

Weight (kDa)

6.17

Isoelectric Point (pI)

26.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 30 - 274 5.6e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 587
AciI CCGC 2 cut(s) 266, 269
AcsI RAATTY 3 cut(s) 157, 737, 797
AcuI CTGAAG 1 cut(s) 492
AcyI GRCGYC 1 cut(s) 781
AfaI GTAC 3 cut(s) 170, 399, 554
AfiI CCNNNNNNNGG 1 cut(s) 170
AflII CTTAAG 2 cut(s) 308, 605
AgsI TTSAA 3 cut(s) 409, 712, 742
AleI CACNNNNGTG 1 cut(s) 89
AluBI AGCT 6 cut(s) 8, 250, 452, 463, 476, 760
AluI AGCT 6 cut(s) 8, 250, 452, 463, 476, 760
Alw21I GWGCWC 1 cut(s) 631
Alw26I GTCTC 1 cut(s) 370
Alw44I GTGCAC 1 cut(s) 627
AoxI GGCC 1 cut(s) 700
ApaLI GTGCAC 1 cut(s) 627
ApeKI GCWGC 5 cut(s) 53, 62, 452, 498, 666
ApoI RAATTY 3 cut(s) 157, 737, 797
AseI ATTAAT 1 cut(s) 864
AspS9I GGNCC 1 cut(s) 656
AsuC2I CCSGG 1 cut(s) 202
AsuHPI GGTGA 3 cut(s) 110, 242, 255
AvaII GGWCC 1 cut(s) 656
BaeGI GKGCMC 1 cut(s) 631
BanI GGYRCC 1 cut(s) 587
Bbv12I GWGCWC 1 cut(s) 631
BbvI GCAGC 5 cut(s) 49, 65, 439, 510, 653
BccI CCATC 4 cut(s) 13, 41, 268, 536
BclI TGATCA 1 cut(s) 391
BcnI CCSGG 1 cut(s) 202
BcoDI GTCTC 1 cut(s) 370
BfaI CTAG 1 cut(s) 26
BfrI CTTAAG 2 cut(s) 308, 605
BisI GCNGC 6 cut(s) 54, 63, 269, 453, 499, 667
BlsI GCNGC 6 cut(s) 55, 64, 270, 454, 500, 668
Bme1390I CCNGG 1 cut(s) 202
Bme18I GGWCC 1 cut(s) 656
BmgT120I GGNCC 1 cut(s) 656
BmiI GGNNCC 1 cut(s) 589
BmrFI CCNGG 1 cut(s) 202
BmsI GCATC 4 cut(s) 74, 83, 289, 335
BpmI CTGGAG 1 cut(s) 63
BpuMI CCSGG 1 cut(s) 202
BsaHI GRCGYC 1 cut(s) 781
BsaJI CCNNGG 2 cut(s) 57, 164
Bsc4I CCNNNNNNNGG 1 cut(s) 170
Bse1I ACTGG 2 cut(s) 361, 619
Bse3DI GCAATG 2 cut(s) 508, 762
BseDI CCNNGG 2 cut(s) 57, 164
BseGI GGATG 1 cut(s) 24
BseLI CCNNNNNNNGG 1 cut(s) 170
BseMI GCAATG 2 cut(s) 508, 762
BseMII CTCAG 1 cut(s) 692
BseNI ACTGG 2 cut(s) 361, 619
BseSI GKGCMC 1 cut(s) 631
BseXI GCAGC 5 cut(s) 49, 65, 439, 510, 653
BsgI GTGCAG 1 cut(s) 357
BshFI GGCC 1 cut(s) 702
BshNI GGYRCC 1 cut(s) 587
BsiHKAI GWGCWC 1 cut(s) 631
BsiSI CCGG 1 cut(s) 201
BslI CCNNNNNNNGG 1 cut(s) 170
BsmAI GTCTC 1 cut(s) 370
BsmI GAATGC 1 cut(s) 669
BsnI GGCC 1 cut(s) 702
Bsp1286I GDGCHC 1 cut(s) 631
Bsp143I GATC 1 cut(s) 391
Bsp19I CCATGG 1 cut(s) 57
BspACI CCGC 2 cut(s) 266, 269
BspANI GGCC 1 cut(s) 702
BspCNI CTCAG 1 cut(s) 691
BspHI TCATGA 1 cut(s) 559
BspLI GGNNCC 1 cut(s) 589
BspT107I GGYRCC 1 cut(s) 587
BspTI CTTAAG 2 cut(s) 308, 605
BsrDI GCAATG 2 cut(s) 508, 762
BsrI ACTGG 2 cut(s) 361, 619
BssECI CCNNGG 2 cut(s) 57, 164
BssMI GATC 1 cut(s) 391
BssNI GRCGYC 1 cut(s) 781
BssT1I CCWWGG 2 cut(s) 57, 164
Bst4CI ACNGT 2 cut(s) 381, 579
BstACI GRCGYC 1 cut(s) 781
BstAFI CTTAAG 2 cut(s) 308, 605
BstDEI CTNAG 3 cut(s) 435, 592, 678
BstDSI CCRYGG 1 cut(s) 57
BstF5I GGATG 1 cut(s) 24
BstKTI GATC 1 cut(s) 394
BstMAI GTCTC 1 cut(s) 370
BstMBI GATC 1 cut(s) 391
BstMWI GCNNNNNNNGC 4 cut(s) 59, 62, 71, 80
BstSCI CCNGG 1 cut(s) 200
BstSLI GKGCMC 1 cut(s) 631
BstV1I GCAGC 5 cut(s) 49, 65, 439, 510, 653
BsuRI GGCC 1 cut(s) 702
BtgI CCRYGG 1 cut(s) 57
BtsCI GGATG 1 cut(s) 24
BtsI GCAGTG 1 cut(s) 302
BtsIMutI CAGTG 2 cut(s) 302, 354
CciI TCATGA 1 cut(s) 559
Cfr13I GGNCC 1 cut(s) 656
CseI GACGC 1 cut(s) 789
Csp6I GTAC 3 cut(s) 169, 398, 553
CviAII CATG 4 cut(s) 49, 58, 560, 647
CviQI GTAC 3 cut(s) 169, 398, 553
DdeI CTNAG 3 cut(s) 435, 592, 678
DpnI GATC 1 cut(s) 393
DpnII GATC 1 cut(s) 391
Eco130I CCWWGG 2 cut(s) 57, 164
Eco47I GGWCC 1 cut(s) 656
Eco57I CTGAAG 1 cut(s) 492
EcoRI GAATTC 1 cut(s) 737
EcoT14I CCWWGG 2 cut(s) 57, 164
EcoT22I ATGCAT 1 cut(s) 328
ErhI CCWWGG 2 cut(s) 57, 164
FaeI CATG 4 cut(s) 52, 61, 563, 650
FatI CATG 4 cut(s) 48, 57, 559, 646
FauNDI CATATG 1 cut(s) 856
FbaI TGATCA 1 cut(s) 391
Fnu4HI GCNGC 6 cut(s) 54, 63, 269, 453, 499, 667
FokI GGATG 1 cut(s) 31
Fsp4HI GCNGC 6 cut(s) 54, 63, 269, 453, 499, 667
FspBI CTAG 1 cut(s) 26
GluI GCNGC 6 cut(s) 54, 63, 269, 453, 499, 667
GsuI CTGGAG 1 cut(s) 63
HaeIII GGCC 1 cut(s) 702
HapII CCGG 1 cut(s) 201
HgaI GACGC 1 cut(s) 789
Hin1I GRCGYC 1 cut(s) 781
Hin1II CATG 4 cut(s) 52, 61, 563, 650
HincII GTYRAC 4 cut(s) 108, 288, 319, 721
HindII GTYRAC 4 cut(s) 108, 288, 319, 721
HindIII AAGCTT 2 cut(s) 248, 461
HinfI GANTC 5 cut(s) 101, 207, 256, 360, 610
HpaI GTTAAC 1 cut(s) 108
HpaII CCGG 1 cut(s) 201
HphI GGTGA 3 cut(s) 110, 242, 255
Hpy166II GTNNAC 7 cut(s) 108, 288, 319, 555, 629, 659, 721
Hpy188I TCNGA 3 cut(s) 70, 681, 823
Hpy188III TCNNGA 4 cut(s) 16, 548, 560, 830
Hpy8I GTNNAC 7 cut(s) 108, 288, 319, 555, 629, 659, 721
HpyAV CCTTC 2 cut(s) 179, 684
HpyCH4III ACNGT 2 cut(s) 381, 579
HpyF10VI GCNNNNNNNGC 4 cut(s) 59, 62, 71, 80
HpyF3I CTNAG 3 cut(s) 435, 592, 678
Hsp92I GRCGYC 1 cut(s) 781
Hsp92II CATG 4 cut(s) 52, 61, 563, 650
Ksp22I TGATCA 1 cut(s) 391
KspAI GTTAAC 1 cut(s) 108
Kzo9I GATC 1 cut(s) 391
LpnPI CCDG 9 cut(s) 93, 179, 214, 315, 324, 342, 552, 632, 719
Lsp1109I GCAGC 5 cut(s) 49, 65, 439, 510, 653
LweI GCATC 4 cut(s) 74, 83, 289, 335
MaeI CTAG 1 cut(s) 26
MaeIII GTNAC 1 cut(s) 98
MalI GATC 1 cut(s) 393
MboI GATC 1 cut(s) 391
MboII GAAGA 2 cut(s) 231, 724
MhlI GDGCHC 1 cut(s) 631
MluCI AATT 6 cut(s) 127, 157, 492, 517, 737, 797
MlyI GAGTC 3 cut(s) 95, 354, 604
MnlI CCTC 3 cut(s) 675, 778, 794
Mph1103I ATGCAT 1 cut(s) 328
MslI CAYNNNNRTG 1 cut(s) 89
MspCI CTTAAG 2 cut(s) 308, 605
MspI CCGG 1 cut(s) 201
MspR9I CCNGG 1 cut(s) 202
Mva1269I GAATGC 1 cut(s) 669
MwoI GCNNNNNNNGC 4 cut(s) 59, 62, 71, 80
NciI CCSGG 1 cut(s) 202
NcoI CCATGG 1 cut(s) 57
NdeI CATATG 1 cut(s) 856
NdeII GATC 1 cut(s) 391
NlaIII CATG 4 cut(s) 52, 61, 563, 650
NlaIV GGNNCC 1 cut(s) 589
NmeAIII GCCGAG 1 cut(s) 642
NmuCI GTSAC 1 cut(s) 98
NsiI ATGCAT 1 cut(s) 328
OliI CACNNNNGTG 1 cut(s) 89
PagI TCATGA 1 cut(s) 559
PctI GAATGC 1 cut(s) 669
PfeI GAWTC 2 cut(s) 207, 256
PkrI GCNGC 6 cut(s) 55, 64, 270, 454, 500, 668
PleI GAGTC 3 cut(s) 95, 354, 604
PpsI GAGTC 3 cut(s) 95, 354, 604
PshBI ATTAAT 1 cut(s) 864
PspN4I GGNNCC 1 cut(s) 589
PspPI GGNCC 1 cut(s) 656
RsaI GTAC 3 cut(s) 170, 399, 554
RsaNI GTAC 3 cut(s) 169, 398, 553
RseI CAYNNNNRTG 1 cut(s) 89
SatI GCNGC 6 cut(s) 54, 63, 269, 453, 499, 667
Sau3AI GATC 1 cut(s) 391
Sau96I GGNCC 1 cut(s) 656
SchI GAGTC 3 cut(s) 95, 354, 604
ScrFI CCNGG 1 cut(s) 202
SduI GDGCHC 1 cut(s) 631
SetI ASST 9 cut(s) 10, 190, 252, 454, 465, 478, 541, 593, 762
SfaNI GCATC 4 cut(s) 74, 83, 289, 335
SinI GGWCC 1 cut(s) 656
SmiMI CAYNNNNRTG 1 cut(s) 89
SmlI CTYRAG 2 cut(s) 308, 605
SmoI CTYRAG 2 cut(s) 308, 605
Sse9I AATT 6 cut(s) 127, 157, 492, 517, 737, 797
SsiI CCGC 2 cut(s) 266, 269
SspMI CTAG 1 cut(s) 26
StyD4I CCNGG 1 cut(s) 200
StyI CCWWGG 2 cut(s) 57, 164
TaaI ACNGT 2 cut(s) 381, 579
TaqI TCGA 1 cut(s) 177
TasI AATT 6 cut(s) 127, 157, 492, 517, 737, 797
TatI WGTACW 2 cut(s) 397, 552
TauI GCSGC 1 cut(s) 271
TfiI GAWTC 2 cut(s) 207, 256
TscAI CASTG 2 cut(s) 309, 361
TseFI GTSAC 1 cut(s) 98
TseI GCWGC 5 cut(s) 53, 62, 452, 498, 666
Tsp45I GTSAC 1 cut(s) 98
TspDTI ATGAA 3 cut(s) 198, 475, 635
TspRI CASTG 2 cut(s) 309, 361
Vha464I CTTAAG 2 cut(s) 308, 605
VneI GTGCAC 1 cut(s) 627
VpaK11BI GGWCC 1 cut(s) 656
VspI ATTAAT 1 cut(s) 864
XapI RAATTY 3 cut(s) 157, 737, 797
XspI CTAG 1 cut(s) 26
Zsp2I ATGCAT 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.