RchiOBHm_Chr3g0490271

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0008491
Physical Location & Seq
Forward (+)
36960741 .. 36962529
1789 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45345

Sequence Viewer

Length: 1059 bp
ATGGAAGCTGTATTCGGGATGGCTCTAGCCACCATCATTTTGACAAGCATGGCAGCCATGGCTGCATCAGAAGCATCTCCAGCCACATTTGTGTTCGGTGACTCGTTAACAGAAGTGGGAAACAACAATTATTTGCAGTATTCTCTTGCAAAGTCAAATTTTCCTTGGTACGGGGTCGATTATGAAGGTGGTCAGGCTACCGGGAGATTCACAAATGGAAGAACTATTGGTGATTTAATATCTGCAAAGCTTGGGATTCCATCACCGCCGCCTTATCTCTCATTGTCAACGAATGATGATGCACTGCTTAAGGGGGTCAACTATGCATCTGGTGGTGCAGGAATACTAAATGACACTGGACTCTATTTTATTGAGAGACTGTCCTTTGATGATCAAGTACAAAGTTTCAACAAGACAAGGGAAGTCATCAAGGCTAAGATTGGGAGTGAAGCTGCAACCAAGCTTTTAACTGAAGCTCTCTATTTCATTGGAATTGGCAGCAATGATTATGTCAATAATTTCTTACAACCCTTTTTACCTGATGGTCAAGAGTACACTCATGATGACTTTGTGGAACTGTTAATAGGCACCTTAGAGCAACAACTTAAGTTGGGTGCACGAAAGATGATGTTTCATGGTCTTGGTCCACTCGGCTGCATTCCTTCTCAGAGGGTTAAATCAAAGAAAGGCCAATGCTTGAAGAAAGTCAACAAGTGGGTGCTGGAATTCAACTCTAAAGTAGAGAAGCTCATTGCCTCTCTTAACAGACGCCTCCCAAAAGCAAAATTCTCATTTGCTGATACATATTCAGATGTTCTTGACTTAATCAGCAACCCTACCACATATGGTTTTAAGGTTTCCAATACGTCATGTTGCAATGTAGACACGAGCATTGGAGGGCTATGCTTACCAAATTCAAAAATATGCACGAACCGCAAAGATTATGTATTCTGGGATGCATTCCACCCCTCAGATGCAGCAAATGCAGTTCTTGCTGACAAACTCTTCTTGAGACTATTTTCTAAGCCTCCAGCCCCTGCACCAACACCTTCTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

352

Amino Acids

38.38

Weight (kDa)

7.55

Isoelectric Point (pI)

27.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 30 - 335 1.9e-39 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 587
AccI GTMKAC 1 cut(s) 882
AciI CCGC 3 cut(s) 266, 269, 934
AcsI RAATTY 4 cut(s) 157, 725, 785, 913
AcuI CTGAAG 1 cut(s) 492
AcyI GRCGYC 1 cut(s) 769
AfaI GTAC 3 cut(s) 170, 399, 554
AfiI CCNNNNNNNGG 1 cut(s) 170
AflII CTTAAG 2 cut(s) 308, 605
AgsI TTSAA 4 cut(s) 409, 700, 730, 918
AleI CACNNNNGTG 1 cut(s) 89
AluBI AGCT 6 cut(s) 8, 250, 452, 463, 476, 748
AluI AGCT 6 cut(s) 8, 250, 452, 463, 476, 748
Alw21I GWGCWC 1 cut(s) 619
Alw26I GTCTC 2 cut(s) 370, 1006
Alw44I GTGCAC 1 cut(s) 615
AlwNI CAGNNNCTG 1 cut(s) 1037
AoxI GGCC 1 cut(s) 688
ApaLI GTGCAC 1 cut(s) 615
ApeKI GCWGC 6 cut(s) 53, 62, 452, 498, 654, 977
ApoI RAATTY 4 cut(s) 157, 725, 785, 913
AspS9I GGNCC 1 cut(s) 644
AsuC2I CCSGG 1 cut(s) 202
AsuHPI GGTGA 3 cut(s) 110, 242, 255
AvaII GGWCC 1 cut(s) 644
BaeGI GKGCMC 1 cut(s) 619
BanI GGYRCC 1 cut(s) 587
BauI CACGAG 1 cut(s) 886
Bbv12I GWGCWC 1 cut(s) 619
BbvI GCAGC 6 cut(s) 49, 65, 439, 510, 641, 989
BccI CCATC 4 cut(s) 13, 41, 268, 536
BclI TGATCA 1 cut(s) 391
BcnI CCSGG 1 cut(s) 202
BcoDI GTCTC 2 cut(s) 370, 1006
BfaI CTAG 1 cut(s) 26
BfrI CTTAAG 2 cut(s) 308, 605
BisI GCNGC 7 cut(s) 54, 63, 269, 453, 499, 655, 978
BlsI GCNGC 7 cut(s) 55, 64, 270, 454, 500, 656, 979
Bme1390I CCNGG 1 cut(s) 202
Bme18I GGWCC 1 cut(s) 644
BmgT120I GGNCC 1 cut(s) 644
BmiI GGNNCC 1 cut(s) 589
BmrFI CCNGG 1 cut(s) 202
BmsI GCATC 6 cut(s) 74, 83, 289, 335, 946, 964
BpmI CTGGAG 2 cut(s) 63, 1014
BpuEI CTTGAG 1 cut(s) 1030
BpuMI CCSGG 1 cut(s) 202
BsaHI GRCGYC 1 cut(s) 769
BsaJI CCNNGG 2 cut(s) 57, 164
Bsc4I CCNNNNNNNGG 1 cut(s) 170
Bse1I ACTGG 1 cut(s) 361
Bse3DI GCAATG 3 cut(s) 508, 750, 883
BseDI CCNNGG 2 cut(s) 57, 164
BseGI GGATG 2 cut(s) 24, 961
BseLI CCNNNNNNNGG 1 cut(s) 170
BseMI GCAATG 3 cut(s) 508, 750, 883
BseMII CTCAG 2 cut(s) 680, 984
BseNI ACTGG 1 cut(s) 361
BseSI GKGCMC 1 cut(s) 619
BseXI GCAGC 6 cut(s) 49, 65, 439, 510, 641, 989
BsgI GTGCAG 2 cut(s) 357, 1023
BshFI GGCC 1 cut(s) 690
BshNI GGYRCC 1 cut(s) 587
BsiHKAI GWGCWC 1 cut(s) 619
BsiSI CCGG 1 cut(s) 201
BslI CCNNNNNNNGG 1 cut(s) 170
BsmAI GTCTC 2 cut(s) 370, 1006
BsmI GAATGC 2 cut(s) 657, 959
BsnI GGCC 1 cut(s) 690
Bsp1286I GDGCHC 1 cut(s) 619
Bsp143I GATC 1 cut(s) 391
Bsp19I CCATGG 1 cut(s) 57
BspACI CCGC 3 cut(s) 266, 269, 934
BspANI GGCC 1 cut(s) 690
BspCNI CTCAG 2 cut(s) 679, 983
BspHI TCATGA 1 cut(s) 559
BspLI GGNNCC 1 cut(s) 589
BspT107I GGYRCC 1 cut(s) 587
BspTI CTTAAG 2 cut(s) 308, 605
BsrDI GCAATG 3 cut(s) 508, 750, 883
BsrI ACTGG 1 cut(s) 361
BssECI CCNNGG 2 cut(s) 57, 164
BssMI GATC 1 cut(s) 391
BssNI GRCGYC 1 cut(s) 769
BssSI CACGAG 1 cut(s) 886
BssT1I CCWWGG 2 cut(s) 57, 164
Bst2BI CACGAG 1 cut(s) 886
Bst4CI ACNGT 2 cut(s) 381, 579
Bst6I CTCTTC 1 cut(s) 1010
BstACI GRCGYC 1 cut(s) 769
BstAFI CTTAAG 2 cut(s) 308, 605
BstAPI GCANNNNNTGC 2 cut(s) 983, 992
BstDEI CTNAG 5 cut(s) 435, 592, 666, 970, 1023
BstDSI CCRYGG 1 cut(s) 57
BstF5I GGATG 2 cut(s) 24, 961
BstKTI GATC 1 cut(s) 394
BstMAI GTCTC 2 cut(s) 370, 1006
BstMBI GATC 1 cut(s) 391
BstMWI GCNNNNNNNGC 7 cut(s) 59, 62, 71, 80, 933, 983, 992
BstSCI CCNGG 1 cut(s) 200
BstSLI GKGCMC 1 cut(s) 619
BstV1I GCAGC 6 cut(s) 49, 65, 439, 510, 641, 989
BsuRI GGCC 1 cut(s) 690
BtgI CCRYGG 1 cut(s) 57
BtsCI GGATG 2 cut(s) 24, 961
BtsI GCAGTG 1 cut(s) 302
BtsIMutI CAGTG 2 cut(s) 302, 354
CaiI CAGNNNCTG 1 cut(s) 1037
CciI TCATGA 1 cut(s) 559
Cfr13I GGNCC 1 cut(s) 644
CseI GACGC 1 cut(s) 777
Csp6I GTAC 3 cut(s) 169, 398, 553
CviAII CATG 5 cut(s) 49, 58, 560, 635, 870
CviQI GTAC 3 cut(s) 169, 398, 553
DdeI CTNAG 5 cut(s) 435, 592, 666, 970, 1023
DpnI GATC 1 cut(s) 393
DpnII GATC 1 cut(s) 391
Eam1104I CTCTTC 1 cut(s) 1010
EarI CTCTTC 1 cut(s) 1010
Eco130I CCWWGG 2 cut(s) 57, 164
Eco47I GGWCC 1 cut(s) 644
Eco57I CTGAAG 1 cut(s) 492
EcoRI GAATTC 1 cut(s) 725
EcoT14I CCWWGG 2 cut(s) 57, 164
EcoT22I ATGCAT 2 cut(s) 328, 961
ErhI CCWWGG 2 cut(s) 57, 164
FaeI CATG 5 cut(s) 52, 61, 563, 638, 873
FatI CATG 5 cut(s) 48, 57, 559, 634, 869
FauNDI CATATG 1 cut(s) 844
FbaI TGATCA 1 cut(s) 391
FblI GTMKAC 1 cut(s) 882
Fnu4HI GCNGC 7 cut(s) 54, 63, 269, 453, 499, 655, 978
FokI GGATG 2 cut(s) 31, 968
Fsp4HI GCNGC 7 cut(s) 54, 63, 269, 453, 499, 655, 978
FspBI CTAG 1 cut(s) 26
GluI GCNGC 7 cut(s) 54, 63, 269, 453, 499, 655, 978
GsuI CTGGAG 2 cut(s) 63, 1014
HaeIII GGCC 1 cut(s) 690
HapII CCGG 1 cut(s) 201
HgaI GACGC 1 cut(s) 777
Hin1I GRCGYC 1 cut(s) 769
Hin1II CATG 5 cut(s) 52, 61, 563, 638, 873
HincII GTYRAC 4 cut(s) 108, 288, 319, 709
HindII GTYRAC 4 cut(s) 108, 288, 319, 709
HindIII AAGCTT 2 cut(s) 248, 461
HinfI GANTC 4 cut(s) 101, 207, 256, 360
HpaI GTTAAC 1 cut(s) 108
HpaII CCGG 1 cut(s) 201
HphI GGTGA 3 cut(s) 110, 242, 255
Hpy166II GTNNAC 8 cut(s) 108, 288, 319, 555, 617, 647, 709, 883
Hpy188I TCNGA 4 cut(s) 70, 669, 811, 973
Hpy188III TCNNGA 5 cut(s) 16, 548, 560, 818, 1009
Hpy8I GTNNAC 8 cut(s) 108, 288, 319, 555, 617, 647, 709, 883
HpyAV CCTTC 3 cut(s) 179, 672, 1059
HpyCH4III ACNGT 2 cut(s) 381, 579
HpyCH4IV ACGT 1 cut(s) 866
HpyF10VI GCNNNNNNNGC 7 cut(s) 59, 62, 71, 80, 933, 983, 992
HpyF3I CTNAG 5 cut(s) 435, 592, 666, 970, 1023
HpySE526I ACGT 1 cut(s) 866
Hsp92I GRCGYC 1 cut(s) 769
Hsp92II CATG 5 cut(s) 52, 61, 563, 638, 873
Ksp22I TGATCA 1 cut(s) 391
KspAI GTTAAC 1 cut(s) 108
Kzo9I GATC 1 cut(s) 391
Lsp1109I GCAGC 6 cut(s) 49, 65, 439, 510, 641, 989
LweI GCATC 6 cut(s) 74, 83, 289, 335, 946, 964
MaeI CTAG 1 cut(s) 26
MaeII ACGT 1 cut(s) 866
MaeIII GTNAC 1 cut(s) 98
MalI GATC 1 cut(s) 393
MboI GATC 1 cut(s) 391
MboII GAAGA 3 cut(s) 231, 712, 997
MhlI GDGCHC 1 cut(s) 619
MluCI AATT 7 cut(s) 127, 157, 492, 517, 725, 785, 913
MlyI GAGTC 2 cut(s) 95, 354
MnlI CCTC 6 cut(s) 663, 766, 782, 890, 979, 1038
Mph1103I ATGCAT 2 cut(s) 328, 961
MslI CAYNNNNRTG 1 cut(s) 89
MspCI CTTAAG 2 cut(s) 308, 605
MspI CCGG 1 cut(s) 201
MspR9I CCNGG 1 cut(s) 202
Mva1269I GAATGC 2 cut(s) 657, 959
MwoI GCNNNNNNNGC 7 cut(s) 59, 62, 71, 80, 933, 983, 992
NciI CCSGG 1 cut(s) 202
NcoI CCATGG 1 cut(s) 57
NdeI CATATG 1 cut(s) 844
NdeII GATC 1 cut(s) 391
NlaIII CATG 5 cut(s) 52, 61, 563, 638, 873
NlaIV GGNNCC 1 cut(s) 589
NmeAIII GCCGAG 1 cut(s) 630
NmuCI GTSAC 1 cut(s) 98
NsiI ATGCAT 2 cut(s) 328, 961
OliI CACNNNNGTG 1 cut(s) 89
PagI TCATGA 1 cut(s) 559
PctI GAATGC 2 cut(s) 657, 959
PfeI GAWTC 2 cut(s) 207, 256
PkrI GCNGC 7 cut(s) 55, 64, 270, 454, 500, 656, 979
PleI GAGTC 2 cut(s) 95, 354
PpsI GAGTC 2 cut(s) 95, 354
PspN4I GGNNCC 1 cut(s) 589
PspPI GGNCC 1 cut(s) 644
PstNI CAGNNNCTG 1 cut(s) 1037
RsaI GTAC 3 cut(s) 170, 399, 554
RsaNI GTAC 3 cut(s) 169, 398, 553
RseI CAYNNNNRTG 1 cut(s) 89
SatI GCNGC 7 cut(s) 54, 63, 269, 453, 499, 655, 978
Sau3AI GATC 1 cut(s) 391
Sau96I GGNCC 1 cut(s) 644
SchI GAGTC 2 cut(s) 95, 354
ScrFI CCNGG 1 cut(s) 202
SduI GDGCHC 1 cut(s) 619
SfaNI GCATC 6 cut(s) 74, 83, 289, 335, 946, 964
SinI GGWCC 1 cut(s) 644
SmiMI CAYNNNNRTG 1 cut(s) 89
SmlI CTYRAG 3 cut(s) 308, 605, 1009
SmoI CTYRAG 3 cut(s) 308, 605, 1009
Sse9I AATT 7 cut(s) 127, 157, 492, 517, 725, 785, 913
SsiI CCGC 3 cut(s) 266, 269, 934
SspMI CTAG 1 cut(s) 26
StyD4I CCNGG 1 cut(s) 200
StyI CCWWGG 2 cut(s) 57, 164
TaaI ACNGT 2 cut(s) 381, 579
TaiI ACGT 1 cut(s) 869
TaqI TCGA 1 cut(s) 177
TasI AATT 7 cut(s) 127, 157, 492, 517, 725, 785, 913
TatI WGTACW 2 cut(s) 397, 552
TauI GCSGC 1 cut(s) 271
TfiI GAWTC 2 cut(s) 207, 256
TscAI CASTG 2 cut(s) 309, 361
TseFI GTSAC 1 cut(s) 98
TseI GCWGC 6 cut(s) 53, 62, 452, 498, 654, 977
Tsp45I GTSAC 1 cut(s) 98
TspDTI ATGAA 3 cut(s) 198, 475, 623
TspRI CASTG 2 cut(s) 309, 361
Vha464I CTTAAG 2 cut(s) 308, 605
VneI GTGCAC 1 cut(s) 615
VpaK11BI GGWCC 1 cut(s) 644
XapI RAATTY 4 cut(s) 157, 725, 785, 913
XmiI GTMKAC 1 cut(s) 882
XspI CTAG 1 cut(s) 26
Zsp2I ATGCAT 2 cut(s) 328, 961
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.