RLG00000022891

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0008491
Physical Location & Seq
Reverse (-)
14189261 .. 14191006
1746 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022891

Sequence Viewer

Length: 1071 bp
ATGGAAGCTGTATTCGGGATGGCTCTAGCCACCATCATTTTGACAAGCATGGCAACCATGGCGGCATCAGCAGCATCTCCAGCCACATTTGTGTTTGGTGACTCGTTAACAGAAGTGGGAAACAACAATTATTTCCAGTATTCTCTTGCAAAGTCAAATTTTCCTTGGTACGGGGTCGATTATGAAGGTGGTCAGGCTACCGGGAGATTCACAAATGGAAGAACTATTGGTGATATAATATCTGCAAAGCTTGGGATTCCATCACCGCCGCCTTATCTCTCATTGTCAACGAATGATGATGCACTGCTTAAGGGGGTCAACTATGCATCTGGTGGAGCAGGAATACTAAATGACACGGGACTCTATTTTATTGAGAGACTGTCCTTTGATGATCAAGTACAAAGTTTCAACAAGACAAGGGAAGTCATCAAGGCTAAGATTGGGAGTGAAGCTGCAACCAAGCTTTTAACTGAAGCTCTCTATTTCATTGGAATTGGCAGCAATGATTATGTCAATAATTTCTTACAACCCTTTTTACCTGATGGTCAAGAGTACACTCATGATGACTTTGTGGAACTGTTAATAGGCGCCTTAGAGCAACAACTTACGACTCTCTACCAGCTCGGTGCACGAAAGATGATGTTTCATGGTCTTGGTCCACTCGGCTGCATTCCTTCTCAGAGGGTTAAATCAAAGAAAGGCCATTGCTTGAAGAAAGTCAATACGTGGGTGCTGGAATTCAACTCTAAAGTAAAGAAGCTCATTGCCTCTCTTAACAGACGCCTCCCAAAAGCAAAATTATCATTTGCAGATACATATTCAGATGTTCTTGACTTAATCAGCAACCCTACCACATATGGTTTTAAGGTTTCCAATACGTCATGTTGCAATGTAGACACGAGCATTGGAGGGCTATGCTTACCGAATTCAAAAATATGCAGCAACCGCAAAGATTATGTGTTCTGGGATGCATTCCACCCGTCAGATGCAGCAAATGCAGTTCTTGCTGACAAACTCTTCTTGAGACTATTTTCTAAGCCTCCAACCCCTGCACCAACACCTTCTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

357

Amino Acids

38.76

Weight (kDa)

8.09

Isoelectric Point (pI)

24.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 30 - 339 1.2e-38 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 587
AccI GTMKAC 1 cut(s) 894
AciI CCGC 4 cut(s) 62, 266, 269, 946
AcsI RAATTY 3 cut(s) 157, 737, 925
AcuI CTGAAG 1 cut(s) 492
AcyI GRCGYC 2 cut(s) 588, 781
AfaI GTAC 3 cut(s) 170, 399, 554
AfiI CCNNNNNNNGG 1 cut(s) 170
AflII CTTAAG 1 cut(s) 308
AgsI TTSAA 4 cut(s) 409, 712, 742, 930
AleI CACNNNNGTG 1 cut(s) 89
AluBI AGCT 7 cut(s) 8, 250, 452, 463, 476, 622, 760
AluI AGCT 7 cut(s) 8, 250, 452, 463, 476, 622, 760
Alw21I GWGCWC 1 cut(s) 631
Alw26I GTCTC 2 cut(s) 370, 1018
Alw44I GTGCAC 1 cut(s) 627
AoxI GGCC 1 cut(s) 700
ApaLI GTGCAC 1 cut(s) 627
ApeKI GCWGC 6 cut(s) 71, 452, 498, 666, 939, 989
ApoI RAATTY 3 cut(s) 157, 737, 925
AspLEI GCGC 1 cut(s) 590
AspS9I GGNCC 1 cut(s) 656
AsuC2I CCSGG 1 cut(s) 202
AsuHPI GGTGA 3 cut(s) 110, 242, 255
AvaII GGWCC 1 cut(s) 656
BaeGI GKGCMC 1 cut(s) 631
BanI GGYRCC 1 cut(s) 587
BauI CACGAG 1 cut(s) 898
Bbv12I GWGCWC 1 cut(s) 631
BbvI GCAGC 6 cut(s) 83, 439, 510, 653, 951, 1001
BccI CCATC 4 cut(s) 13, 41, 268, 536
BclI TGATCA 1 cut(s) 391
BcnI CCSGG 1 cut(s) 202
BcoDI GTCTC 2 cut(s) 370, 1018
BfaI CTAG 1 cut(s) 26
BfoI RGCGCY 1 cut(s) 591
BfrI CTTAAG 1 cut(s) 308
BisI GCNGC 8 cut(s) 63, 72, 269, 453, 499, 667, 940, 990
BlsI GCNGC 8 cut(s) 64, 73, 270, 454, 500, 668, 941, 991
Bme1390I CCNGG 1 cut(s) 202
Bme18I GGWCC 1 cut(s) 656
BmgT120I GGNCC 1 cut(s) 656
BmiI GGNNCC 1 cut(s) 589
BmrFI CCNGG 1 cut(s) 202
BmsI GCATC 6 cut(s) 74, 83, 289, 335, 958, 976
BpmI CTGGAG 1 cut(s) 63
BpuEI CTTGAG 1 cut(s) 1042
BpuMI CCSGG 1 cut(s) 202
BsaAI YACGTR 1 cut(s) 726
BsaHI GRCGYC 2 cut(s) 588, 781
BsaJI CCNNGG 2 cut(s) 57, 164
BsaXI ACNNNNNCTCC 1 cut(s) 1048
Bsc4I CCNNNNNNNGG 1 cut(s) 170
Bse1I ACTGG 1 cut(s) 136
Bse3DI GCAATG 4 cut(s) 508, 703, 762, 895
BseDI CCNNGG 2 cut(s) 57, 164
BseGI GGATG 2 cut(s) 24, 973
BseLI CCNNNNNNNGG 1 cut(s) 170
BseMI GCAATG 4 cut(s) 508, 703, 762, 895
BseMII CTCAG 1 cut(s) 692
BseNI ACTGG 1 cut(s) 136
BseSI GKGCMC 1 cut(s) 631
BseXI GCAGC 6 cut(s) 83, 439, 510, 653, 951, 1001
BsgI GTGCAG 1 cut(s) 1035
BshFI GGCC 1 cut(s) 702
BshNI GGYRCC 1 cut(s) 587
BsiHKAI GWGCWC 1 cut(s) 631
BsiSI CCGG 1 cut(s) 201
BslFI GGGAC 1 cut(s) 372
BslI CCNNNNNNNGG 1 cut(s) 170
BsmAI GTCTC 2 cut(s) 370, 1018
BsmFI GGGAC 1 cut(s) 372
BsmI GAATGC 2 cut(s) 669, 971
BsnI GGCC 1 cut(s) 702
Bsp1286I GDGCHC 1 cut(s) 631
Bsp143I GATC 1 cut(s) 391
Bsp19I CCATGG 1 cut(s) 57
BspACI CCGC 4 cut(s) 62, 266, 269, 946
BspANI GGCC 1 cut(s) 702
BspCNI CTCAG 1 cut(s) 691
BspHI TCATGA 1 cut(s) 559
BspLI GGNNCC 1 cut(s) 589
BspT107I GGYRCC 1 cut(s) 587
BspTI CTTAAG 1 cut(s) 308
BsrDI GCAATG 4 cut(s) 508, 703, 762, 895
BsrI ACTGG 1 cut(s) 136
BssECI CCNNGG 2 cut(s) 57, 164
BssMI GATC 1 cut(s) 391
BssNI GRCGYC 2 cut(s) 588, 781
BssSI CACGAG 1 cut(s) 898
BssT1I CCWWGG 2 cut(s) 57, 164
Bst2BI CACGAG 1 cut(s) 898
Bst4CI ACNGT 2 cut(s) 381, 579
Bst6I CTCTTC 1 cut(s) 1022
BstACI GRCGYC 2 cut(s) 588, 781
BstAFI CTTAAG 1 cut(s) 308
BstAPI GCANNNNNTGC 2 cut(s) 995, 1004
BstBAI YACGTR 1 cut(s) 726
BstDEI CTNAG 4 cut(s) 435, 592, 678, 1035
BstDSI CCRYGG 1 cut(s) 57
BstF5I GGATG 2 cut(s) 24, 973
BstH2I RGCGCY 1 cut(s) 591
BstHHI GCGC 1 cut(s) 590
BstKTI GATC 1 cut(s) 394
BstMAI GTCTC 2 cut(s) 370, 1018
BstMBI GATC 1 cut(s) 391
BstMWI GCNNNNNNNGC 7 cut(s) 59, 68, 71, 80, 945, 995, 1004
BstSCI CCNGG 1 cut(s) 200
BstSLI GKGCMC 1 cut(s) 631
BstV1I GCAGC 6 cut(s) 83, 439, 510, 653, 951, 1001
BsuRI GGCC 1 cut(s) 702
BtgI CCRYGG 1 cut(s) 57
BtsCI GGATG 2 cut(s) 24, 973
BtsI GCAGTG 1 cut(s) 302
BtsIMutI CAGTG 1 cut(s) 302
CciI TCATGA 1 cut(s) 559
CfoI GCGC 1 cut(s) 590
Cfr13I GGNCC 1 cut(s) 656
CseI GACGC 1 cut(s) 789
Csp6I GTAC 3 cut(s) 169, 398, 553
CviAII CATG 5 cut(s) 49, 58, 560, 647, 882
CviQI GTAC 3 cut(s) 169, 398, 553
DdeI CTNAG 4 cut(s) 435, 592, 678, 1035
DinI GGCGCC 1 cut(s) 589
DpnI GATC 1 cut(s) 393
DpnII GATC 1 cut(s) 391
Eam1104I CTCTTC 1 cut(s) 1022
EarI CTCTTC 1 cut(s) 1022
Eco130I CCWWGG 2 cut(s) 57, 164
Eco47I GGWCC 1 cut(s) 656
Eco57I CTGAAG 1 cut(s) 492
EcoRI GAATTC 2 cut(s) 737, 925
EcoT14I CCWWGG 2 cut(s) 57, 164
EcoT22I ATGCAT 2 cut(s) 328, 973
EgeI GGCGCC 1 cut(s) 589
EheI GGCGCC 1 cut(s) 589
ErhI CCWWGG 2 cut(s) 57, 164
FaeI CATG 5 cut(s) 52, 61, 563, 650, 885
FaqI GGGAC 1 cut(s) 372
FatI CATG 5 cut(s) 48, 57, 559, 646, 881
FauNDI CATATG 1 cut(s) 856
FbaI TGATCA 1 cut(s) 391
FblI GTMKAC 1 cut(s) 894
Fnu4HI GCNGC 8 cut(s) 63, 72, 269, 453, 499, 667, 940, 990
FokI GGATG 2 cut(s) 31, 980
Fsp4HI GCNGC 8 cut(s) 63, 72, 269, 453, 499, 667, 940, 990
FspBI CTAG 1 cut(s) 26
GlaI GCGC 1 cut(s) 589
GluI GCNGC 8 cut(s) 63, 72, 269, 453, 499, 667, 940, 990
GsuI CTGGAG 1 cut(s) 63
HaeII RGCGCY 1 cut(s) 591
HaeIII GGCC 1 cut(s) 702
HapII CCGG 1 cut(s) 201
HgaI GACGC 1 cut(s) 789
HhaI GCGC 1 cut(s) 590
Hin1I GRCGYC 2 cut(s) 588, 781
Hin1II CATG 5 cut(s) 52, 61, 563, 650, 885
Hin6I GCGC 1 cut(s) 588
HinP1I GCGC 1 cut(s) 588
HincII GTYRAC 3 cut(s) 108, 288, 319
HindII GTYRAC 3 cut(s) 108, 288, 319
HindIII AAGCTT 2 cut(s) 248, 461
HinfI GANTC 5 cut(s) 101, 207, 256, 360, 610
HpaI GTTAAC 1 cut(s) 108
HpaII CCGG 1 cut(s) 201
HphI GGTGA 3 cut(s) 110, 242, 255
Hpy166II GTNNAC 7 cut(s) 108, 288, 319, 555, 629, 659, 895
Hpy188I TCNGA 3 cut(s) 681, 823, 985
Hpy188III TCNNGA 5 cut(s) 16, 548, 560, 830, 1021
Hpy8I GTNNAC 7 cut(s) 108, 288, 319, 555, 629, 659, 895
HpyAV CCTTC 3 cut(s) 179, 684, 1071
HpyCH4III ACNGT 2 cut(s) 381, 579
HpyCH4IV ACGT 2 cut(s) 725, 878
HpyF10VI GCNNNNNNNGC 7 cut(s) 59, 68, 71, 80, 945, 995, 1004
HpyF3I CTNAG 4 cut(s) 435, 592, 678, 1035
HpySE526I ACGT 2 cut(s) 725, 878
Hsp92I GRCGYC 2 cut(s) 588, 781
Hsp92II CATG 5 cut(s) 52, 61, 563, 650, 885
HspAI GCGC 1 cut(s) 588
KasI GGCGCC 1 cut(s) 587
Ksp22I TGATCA 1 cut(s) 391
KspAI GTTAAC 1 cut(s) 108
Kzo9I GATC 1 cut(s) 391
LmnI GCTCC 1 cut(s) 335
Lsp1109I GCAGC 6 cut(s) 83, 439, 510, 653, 951, 1001
LweI GCATC 6 cut(s) 74, 83, 289, 335, 958, 976
MaeI CTAG 1 cut(s) 26
MaeII ACGT 2 cut(s) 725, 878
MaeIII GTNAC 1 cut(s) 98
MalI GATC 1 cut(s) 393
MboI GATC 1 cut(s) 391
MboII GAAGA 3 cut(s) 231, 724, 1009
MhlI GDGCHC 1 cut(s) 631
MluCI AATT 7 cut(s) 127, 157, 492, 517, 737, 797, 925
Mly113I GGCGCC 1 cut(s) 588
MlyI GAGTC 3 cut(s) 95, 354, 604
MmeI TCCRAC 1 cut(s) 1067
MnlI CCTC 5 cut(s) 675, 778, 794, 902, 1050
Mph1103I ATGCAT 2 cut(s) 328, 973
MseI TTAA 8 cut(s) 107, 309, 467, 581, 687, 774, 836, 864
MslI CAYNNNNRTG 1 cut(s) 89
MspCI CTTAAG 1 cut(s) 308
MspI CCGG 1 cut(s) 201
MspR9I CCNGG 1 cut(s) 202
Mva1269I GAATGC 2 cut(s) 669, 971
MwoI GCNNNNNNNGC 7 cut(s) 59, 68, 71, 80, 945, 995, 1004
NarI GGCGCC 1 cut(s) 588
NciI CCSGG 1 cut(s) 202
NcoI CCATGG 1 cut(s) 57
NdeI CATATG 1 cut(s) 856
NdeII GATC 1 cut(s) 391
NlaIII CATG 5 cut(s) 52, 61, 563, 650, 885
NlaIV GGNNCC 1 cut(s) 589
NmeAIII GCCGAG 1 cut(s) 642
NmuCI GTSAC 1 cut(s) 98
NsiI ATGCAT 2 cut(s) 328, 973
OliI CACNNNNGTG 1 cut(s) 89
PagI TCATGA 1 cut(s) 559
PctI GAATGC 2 cut(s) 669, 971
PfeI GAWTC 2 cut(s) 207, 256
PkrI GCNGC 8 cut(s) 64, 73, 270, 454, 500, 668, 941, 991
PleI GAGTC 3 cut(s) 95, 354, 604
PluTI GGCGCC 1 cut(s) 591
PpsI GAGTC 3 cut(s) 95, 354, 604
Ppu21I YACGTR 1 cut(s) 726
PspN4I GGNNCC 1 cut(s) 589
PspPI GGNCC 1 cut(s) 656
RsaI GTAC 3 cut(s) 170, 399, 554
RsaNI GTAC 3 cut(s) 169, 398, 553
RseI CAYNNNNRTG 1 cut(s) 89
SaqAI TTAA 8 cut(s) 107, 309, 467, 581, 687, 774, 836, 864
SatI GCNGC 8 cut(s) 63, 72, 269, 453, 499, 667, 940, 990
Sau3AI GATC 1 cut(s) 391
Sau96I GGNCC 1 cut(s) 656
SchI GAGTC 3 cut(s) 95, 354, 604
ScrFI CCNGG 1 cut(s) 202
SduI GDGCHC 1 cut(s) 631
SfaNI GCATC 6 cut(s) 74, 83, 289, 335, 958, 976
SfoI GGCGCC 1 cut(s) 589
SinI GGWCC 1 cut(s) 656
SmiMI CAYNNNNRTG 1 cut(s) 89
SmlI CTYRAG 2 cut(s) 308, 1021
SmoI CTYRAG 2 cut(s) 308, 1021
Sse9I AATT 7 cut(s) 127, 157, 492, 517, 737, 797, 925
SsiI CCGC 4 cut(s) 62, 266, 269, 946
SspDI GGCGCC 1 cut(s) 587
SspMI CTAG 1 cut(s) 26
StyD4I CCNGG 1 cut(s) 200
StyI CCWWGG 2 cut(s) 57, 164
TaaI ACNGT 2 cut(s) 381, 579
TaiI ACGT 2 cut(s) 728, 881
TaqI TCGA 1 cut(s) 177
TasI AATT 7 cut(s) 127, 157, 492, 517, 737, 797, 925
TatI WGTACW 2 cut(s) 397, 552
TauI GCSGC 2 cut(s) 65, 271
TfiI GAWTC 2 cut(s) 207, 256
Tru1I TTAA 8 cut(s) 107, 309, 467, 581, 687, 774, 836, 864
Tru9I TTAA 8 cut(s) 107, 309, 467, 581, 687, 774, 836, 864
TscAI CASTG 1 cut(s) 309
TseFI GTSAC 1 cut(s) 98
TseI GCWGC 6 cut(s) 71, 452, 498, 666, 939, 989
Tsp45I GTSAC 1 cut(s) 98
TspDTI ATGAA 3 cut(s) 198, 475, 635
TspRI CASTG 1 cut(s) 309
Vha464I CTTAAG 1 cut(s) 308
VneI GTGCAC 1 cut(s) 627
VpaK11BI GGWCC 1 cut(s) 656
XapI RAATTY 3 cut(s) 157, 737, 925
XmiI GTMKAC 1 cut(s) 894
XspI CTAG 1 cut(s) 26
Zsp2I ATGCAT 2 cut(s) 328, 973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.