FvH4_2g05730

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009109
Physical Location & Seq
Reverse (-)
4696582 .. 4702043
5462 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g05730.t1

Sequence Viewer

Length: 3321 bp
ATGGAGATCGCCCCGGCGCCGCCGCAGTCCGCAGACCGGGACGTCCCAAACGACTCCGCGTCCGTCAAAACGACGCCGTCCTCCTCGCTCTCCGAGAACGACGAGGCGAACGTGTCGACGTCGGTGGCGGCGGCGGCCAAGTACGACGACGACGAAGAGGAGGAGGACGTGTGCCGGATCTGTAGGAACCCTGGCGACGCCGATAACCCGCTCCGGTATCCGTGCGCTTGTAGCGGAAGCATCAAGTTCGTGCACCAGGATTGTCTCCTTCAGTGGCTCAATCACAGCAATGCTCGCCAATGCGAGGTTTGCAAGCATCCATTTTCCTTCTCTCCGGTTTATGCTGAGAATGCACCTGCTAGGCTTCCTTTTCAGGAGTTTGTAGTTGGGATGGCAATGAAAACTTGCCATGTTCTGCAATTTTTTCTGCGCCTTAGCTTTGTGCTATCCGTCTGGCTCCTCATTATACCTTTTATTACGTTTTGGATATGGCGGCTGGCTTTTGTGAGGAGTTTTGGTGAAGCTCAGAGATTGTTCCTAAGTCATCTATCAACTACAGTCATTCTTACTGATTGTCTGCATGGGTTCCTACTTTCTGCGAGCATTGTGTTCATATTTCTTGGAGCCACTTCTCTGAGGGATTACTTCAGGCATTTACGTGAGCTTGGAGGTCAAGATGTTGACAGAGAAGATGAGGCAGAAAGAAATGGTGCTCGTCCTGTAAGAAGAGTTCCTGGACAGGCTAATAGAAACTTTGTTGGCGACGCTAACGGGGAAGATGCTGCTGGAGCACAAGGGATTGCTGGAGCAGGTCAAATGATTAGAAGGAATGCTGAAAATGTGGCCGCTCGCTGGGAGATGCAGGCAGCTCGTCTTGAGGCTCATGTTGAACAGATGTTTGATGGTCTAGACGATGCCGATGGTGCAGAGGATGTACCATTTGACGAGCTTGTGGGAATGCAGGGACCTGTATTTCATTTAGTGGAAAATGCTTTCACTGTGCTGGCAAGCAATATGATATTCCTTGGTGTAGTAATATTTGTGCCCTTCTCATTAGGTCGGATTATACTCTATCATTTGTCTTGGCTTTTCTCCACTGCTAGTGGTCCAGTCTTGTCAACAGTCATGCCACTCACTGAATCAGCCATTTCCTTAGCTAATATCACATTGAAGAATGCATTAACTGCTGTAACAAATTTATCAACCGATGGCGAGAAAAGTGGTATGGCTGGCCAGGTTGCAGAGATCCTGAAAGTTAACATGAGTGGACTGAATGAGGTTTCAAACAATGCAAGTTCACCACTTTCAGCAGACATCCTTAAAGGGGCAACAATTGGAACGTCAAGGCTCTCTGATGTGACAACTCTTGCTATTGGATACATGTTTATATTCTCCCTAGTTTTCTTCTACCTTGGCATTGTGGCTTTTATCCGTTACACTAGGGGTGAGCCATTGACTATGGGAAGGTTCTATGGTATTGCTTCAATGGCAGAGACAATTCCATCTCTATTCAGGCAGTTTCTGGCCGCAATGAGGCATTTGATGACAATGATTAAGGTCGCTTTCCTTCTGGTCATTGAACTTGGAGTGTTCCCATTAATGTGTGGATGGTGGTTGGATGTTTGTACTATAAGGATGTTTGGGAAAACGATGTCTCACAGGGTGCAGTTCTTCTCAGCGTCTCCTTTAGCTAGCTCCCTGGTTCATTGGGTTGTAGGAATTGTATACATGCTACAAATAAGCATATTTGTCAGTCTTCTTCGAGGGGTTCTGCGTAATGGAGTTCTTTATTTCCTAAGAGATCCAGCTGATCCAAACTACAATCCCTTCCGTGATCTCATTGATGATCCTGTGCACAAACATGCTCGCAGGGTTCTGTTATCCGTTGCGGTGTATGGGAGTTTAATTGTGATGCTGGTGTTTTTACCAGTTAAACTAGCTATGCGGATGGCACCTTCCATTTTTCCCCTTGACATTTCGGTATCAGACCCATTTACTGAAATTCCTGCGGACATGCTTTTATTTCAAATCTGCATTCCATTTGCAATTGAACATTTCAAATTACGGGCAACCATTAAATCCCTGCTGCGTTATTGGTTTACGGCAGTTGGCTGGGCACTTGGTTTAACAGATTTCTTACTACCCAGACATGAGGATAACGTCGGACAGGAAAACGGAAATGCAGAGCCAGGAAGACAGGATAGGTTACAGGTTCAATTGGGGCTACAGGATCAAGCTTTGGTGGCCCTGCCAGGTGCTGATGAACCAAATGGAGGGCTTCTGGCATCAGGGGACTCGAATATTGCAGAAGAGTATGACACTGATGAACAATCTGATTCTGAAAGGTATAGCTTTGTACTTCGAATTGTCCTCTTGTTGGTGGTGGCTTGGATGACGTTACTTGTCTTCAACTCAGCCTTGATAGTTGTACCAACATCACTCGGAAGAACAATTTTCAATGTCATTCCTTTCCTCCCTATAACTCATGGCATCAAGTGCAACGATTTATATGCTTTCATCATCGGAAGCTACATAATATGGACTGCTGTAGCTGGAGTCAGATATTCTATTGAGCACATTAGAACAAAAAGGGTTGCCGTTCTATTGGGCCAGATCTGGAAGTGGTGTAGCATTGTCATAAAGAGTTCTGCCTTGTTGTCAATATGGATTTTTGTCATTCCAGTATTGATCGGGTTGCTCTTTGAGCTTTTGGTGATCGTGCCAATGCGGGTGCCTGTTGATGAAAGCCCAGTATTCCTGCTGTATCAGGACTGGGCTTTGGGCCTTATTTTTCTCAAGATATGGACTAGATTGGTGATGTTGGATCACATGATGCCATTGGTAGATGAAAGCTGGCGCGTAAAGTTTGAAAGGGTGAGGGAAGACGGCTTCTCCAGGCTGCAAGGTCTTTGGGTGCTCCGCGAGATTGTATTCCCAATCATAATGAAGTTGTTGACTGCCCTGTGTGTGCCTTACGTTCTTGCCAGAGGGTTATTTCCTGTGCTCGGTTACCCATTAGTAGTCAACTCAGCAGTTTATCGGTTTGCCTGGATAGGATGCCTGTGCTTTAGTCTGCTGTGCTTCTGTGCCAAGAGGTTCCATGTATGGTTCACCAACCTTCACAACTCTATACGTGATGACCGATATCTAATTGGACGAAGGCTTCATAACTTTGGAGAAGATGTTGAAGAAAAGCAAAATGAGGCAGGGACATCCCTTGAATTGCAGGATTCTAGTTTCGAAGTCAGTGGTTTAATTCCACATGACCGAGAGGCTGACCATGGTCTCCGTCTAAGGCGTGCTATTCAACATGATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000209 GO:0001101 GO:0002020 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006508 GO:0006511 GO:0006629 GO:0006720 GO:0006725 GO:0006807 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009414 GO:0009415 GO:0009628 GO:0009698 GO:0009699 GO:0009893 GO:0009987 GO:0010025 GO:0010033 GO:0010035 GO:0010143 GO:0010166 GO:0010243 GO:0010345 GO:0010498 GO:0012505 GO:0016020 GO:0016021 GO:0016567 GO:0016740 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019787 GO:0019899 GO:0019941 GO:0030163 GO:0030176 GO:0030433 GO:0031224 GO:0031227 GO:0031624 GO:0031984 GO:0032446 GO:0032501 GO:0032502 GO:0033554 GO:0034976 GO:0036211 GO:0036503 GO:0042175 GO:0042221 GO:0042335 GO:0043085 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044390 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0048518 GO:0048856 GO:0050789 GO:0050790 GO:0050896 GO:0051341 GO:0051353 GO:0051603 GO:0051716 GO:0065007 GO:0065009 GO:0070647 GO:0070936 GO:0071704 GO:0098827 GO:0140096 GO:1900490 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901568 GO:1901570 GO:1901575 GO:1901576 GO:1901698 GO:1901700 GO:1990381 GO:2001215
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
RINGv

Protein Analysis

1107

Amino Acids

123.73

Weight (kDa)

5.75

Isoelectric Point (pI)

40.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 58 - 104 9.9e-17 RING-variant domain
MARCHF6_C PF23113 867 - 1045 7.9e-51 E3 ubiquitin-protein ligase MARCHF6-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 364
AasI GACNNNNNNGTC 1 cut(s) 41
AatII GACGTC 2 cut(s) 45, 122
Acc36I ACCTGC 2 cut(s) 364, 800
AccB1I GGYRCC 3 cut(s) 16, 1951, 2731
AccBSI CCGCTC 2 cut(s) 211, 848
AccI GTMKAC 2 cut(s) 116, 1725
AccII CGCG 3 cut(s) 59, 2859, 2922
AclWI GGATC 7 cut(s) 185, 1240, 1796, 1805, 1841, 2238, 2832
AcoI YGGCCR 4 cut(s) 135, 843, 1231, 1524
AcsI RAATTY 2 cut(s) 1195, 2001
AcuI CTGAAG 2 cut(s) 254, 631
AcyI GRCGYC 5 cut(s) 17, 42, 74, 119, 198
AdeI CACNNNGTG 1 cut(s) 1663
AfaI GTAC 5 cut(s) 143, 936, 1627, 2358, 2430
AfiI CCNNNNNNNGG 8 cut(s) 36, 304, 852, 1324, 1533, 2273, 2377, 3005
AflIII ACRYGT 3 cut(s) 111, 168, 1380
AhdI GACNNNNNGTC 1 cut(s) 58
AjiI CACGTC 1 cut(s) 169
AjnI CCWGG 9 cut(s) 190, 255, 733, 1233, 1698, 2188, 2251, 2894, 3047
Alw21I GWGCWC 7 cut(s) 255, 715, 793, 1857, 2577, 2919, 3006
Alw26I GTCTC 5 cut(s) 269, 1487, 1659, 1686, 3290
Alw44I GTGCAC 2 cut(s) 251, 1853
AlwI GGATC 7 cut(s) 185, 1240, 1796, 1805, 1841, 2238, 2832
AlwNI CAGNNNCTG 2 cut(s) 1522, 2258
AoxI GGCC 7 cut(s) 135, 843, 1231, 1524, 2244, 2608, 2782
ApaLI GTGCAC 2 cut(s) 251, 1853
ApeKI GCWGC 4 cut(s) 782, 866, 2086, 2899
ApoI RAATTY 2 cut(s) 1195, 2001
AseI ATTAAT 1 cut(s) 1598
AspLEI GCGC 4 cut(s) 19, 227, 432, 2859
AspS9I GGNCC 5 cut(s) 965, 1106, 2245, 2608, 2782
AsuC2I CCSGG 2 cut(s) 14, 38
AsuHPI GGTGA 7 cut(s) 530, 1290, 1457, 2725, 2827, 2887, 3103
AsuII TTCGAA 2 cut(s) 2362, 3240
AsuNHI GCTAGC 1 cut(s) 1691
AvaII GGWCC 2 cut(s) 965, 1106
BaeGI GKGCMC 4 cut(s) 255, 1047, 1857, 2119
BalI TGGCCA 1 cut(s) 1233
BanI GGYRCC 3 cut(s) 16, 1951, 2731
BbsI GAAGAC 4 cut(s) 1748, 2200, 2398, 2889
Bbv12I GWGCWC 7 cut(s) 255, 715, 793, 1857, 2577, 2919, 3006
BbvI GCAGC 4 cut(s) 769, 878, 2073, 2886
BccI CCATC 7 cut(s) 385, 896, 914, 1202, 1510, 1602, 1942
BceAI ACGGC 4 cut(s) 61, 2118, 2582, 2902
BcgI CGANNNNNNTGC 6 cut(s) 229, 263, 851, 885, 2492, 2526
BciT130I CCWGG 9 cut(s) 192, 257, 735, 1235, 1700, 2190, 2253, 2896, 3049
BciVI GTATCC 2 cut(s) 228, 1370
BcnI CCSGG 2 cut(s) 14, 38
BcoDI GTCTC 5 cut(s) 269, 1487, 1659, 1686, 3290
BfaI CTAG 9 cut(s) 360, 908, 1101, 1397, 1440, 1692, 1937, 2808, 3234
BfmI CTRYAG 4 cut(s) 181, 555, 2225, 2547
BfoI RGCGCY 1 cut(s) 20
BfuAI ACCTGC 2 cut(s) 364, 800
BfuI GTATCC 2 cut(s) 228, 1370
BglII AGATCT 1 cut(s) 2613
Bme18I GGWCC 2 cut(s) 965, 1106
BmeRI GACNNNNNGTC 1 cut(s) 58
BmgBI CACGTC 1 cut(s) 169
BmgT120I GGNCC 5 cut(s) 965, 1106, 2245, 2608, 2782
BmiI GGNNCC 9 cut(s) 18, 188, 458, 587, 625, 966, 1953, 2733, 3098
BmrI ACTGGG 2 cut(s) 2744, 2782
BmtI GCTAGC 1 cut(s) 1695
BmuI ACTGGG 2 cut(s) 2744, 2782
BoxI GACNNNNGTC 1 cut(s) 3282
BpiI GAAGAC 4 cut(s) 1748, 2200, 2398, 2889
BpmI CTGGAG 4 cut(s) 807, 825, 2574, 2878
Bpu10I CCTNAGC 2 cut(s) 434, 1153
Bpu14I TTCGAA 2 cut(s) 2362, 3240
BpuEI CTTGAG 2 cut(s) 896, 2780
BpuMI CCSGG 2 cut(s) 14, 38
BsaAI YACGTR 2 cut(s) 659, 3134
BsaHI GRCGYC 5 cut(s) 17, 42, 74, 119, 198
BsaI GGTCTC 1 cut(s) 3290
BsaJI CCNNGG 6 cut(s) 12, 190, 1024, 1411, 1698, 3280
BsaWI WCCGGW 2 cut(s) 213, 334
BsaXI ACNNNNNCTCC 8 cut(s) 155, 185, 502, 532, 2876, 2906, 3168, 3198
Bsc4I CCNNNNNNNGG 8 cut(s) 36, 304, 852, 1324, 1533, 2273, 2377, 3005
Bse1I ACTGG 5 cut(s) 1109, 1928, 2681, 2750, 2777
Bse3DI GCAATG 3 cut(s) 295, 402, 1536
BseBI CCWGG 9 cut(s) 192, 257, 735, 1235, 1700, 2190, 2253, 2896, 3049
BseDI CCNNGG 6 cut(s) 12, 190, 1024, 1411, 1698, 3280
BseLI CCNNNNNNNGG 8 cut(s) 36, 304, 852, 1324, 1533, 2273, 2377, 3005
BseMI GCAATG 3 cut(s) 295, 402, 1536
BseMII CTCAG 6 cut(s) 336, 539, 626, 1689, 2427, 3042
BseNI ACTGG 5 cut(s) 1109, 1928, 2681, 2750, 2777
BseRI GAGGAG 5 cut(s) 73, 173, 176, 449, 523
BseSI GKGCMC 4 cut(s) 255, 1047, 1857, 2119
BseXI GCAGC 4 cut(s) 769, 878, 2073, 2886
BseYI CCCAGC 2 cut(s) 852, 2112
BsgI GTGCAG 2 cut(s) 945, 1685
Bsh1236I CGCG 3 cut(s) 59, 2859, 2922
BshFI GGCC 7 cut(s) 137, 845, 1233, 1526, 2246, 2610, 2784
BshNI GGYRCC 3 cut(s) 16, 1951, 2731
BsiHKAI GWGCWC 7 cut(s) 255, 715, 793, 1857, 2577, 2919, 3006
BsiSI CCGG 5 cut(s) 14, 37, 175, 214, 335
BslFI GGGAC 5 cut(s) 29, 53, 978, 2306, 3223
BslI CCNNNNNNNGG 8 cut(s) 36, 304, 852, 1324, 1533, 2273, 2377, 3005
BsmAI GTCTC 5 cut(s) 269, 1487, 1659, 1686, 3290
BsmBI CGTCTC 1 cut(s) 1686
BsmFI GGGAC 5 cut(s) 29, 53, 978, 2306, 3223
BsmI GAATGC 5 cut(s) 355, 835, 963, 1180, 2034
BsnI GGCC 7 cut(s) 137, 845, 1233, 1526, 2246, 2610, 2784
Bso31I GGTCTC 1 cut(s) 3290
Bsp119I TTCGAA 2 cut(s) 2362, 3240
Bsp1286I GDGCHC 9 cut(s) 255, 715, 793, 1047, 1857, 2119, 2577, 2919, 3006
Bsp19I CCATGG 1 cut(s) 3280
BspANI GGCC 7 cut(s) 137, 845, 1233, 1526, 2246, 2610, 2784
BspCNI CTCAG 6 cut(s) 337, 538, 627, 1688, 2426, 3041
BspFNI CGCG 3 cut(s) 59, 2859, 2922
BspLI GGNNCC 9 cut(s) 18, 188, 458, 587, 625, 966, 1953, 2733, 3098
BspMI ACCTGC 2 cut(s) 364, 800
BspOI GCTAGC 1 cut(s) 1695
BspPI GGATC 7 cut(s) 185, 1240, 1796, 1805, 1841, 2238, 2832
BspT104I TTCGAA 2 cut(s) 2362, 3240
BspT107I GGYRCC 3 cut(s) 16, 1951, 2731
BspTNI GGTCTC 1 cut(s) 3290
BsrBI CCGCTC 2 cut(s) 211, 848
BsrDI GCAATG 3 cut(s) 295, 402, 1536
BsrI ACTGG 5 cut(s) 1109, 1928, 2681, 2750, 2777
BssECI CCNNGG 6 cut(s) 12, 190, 1024, 1411, 1698, 3280
BssNAI GTATAC 1 cut(s) 1726
BssNI GRCGYC 5 cut(s) 17, 42, 74, 119, 198
BssT1I CCWWGG 3 cut(s) 1024, 1411, 3280
Bst1107I GTATAC 1 cut(s) 1726
Bst2UI CCWGG 9 cut(s) 192, 257, 735, 1235, 1700, 2190, 2253, 2896, 3049
Bst4CI ACNGT 3 cut(s) 559, 1000, 1123
Bst6I CTCTTC 3 cut(s) 150, 721, 2304
BstACI GRCGYC 5 cut(s) 17, 42, 74, 119, 198
BstAPI GCANNNNNTGC 2 cut(s) 1184, 2496
BstBAI YACGTR 2 cut(s) 659, 3134
BstBI TTCGAA 2 cut(s) 2362, 3240
BstDSI CCRYGG 1 cut(s) 3280
BstEII GGTNACC 1 cut(s) 3008
BstFNI CGCG 3 cut(s) 59, 2859, 2922
BstH2I RGCGCY 1 cut(s) 20
BstHHI GCGC 4 cut(s) 19, 227, 432, 2859
BstMAI GTCTC 5 cut(s) 269, 1487, 1659, 1686, 3290
BstNI CCWGG 9 cut(s) 192, 257, 735, 1235, 1700, 2190, 2253, 2896, 3049
BstNSI RCATGY 4 cut(s) 1384, 1732, 1865, 2017
BstPAI GACNNNNGTC 1 cut(s) 3282
BstPI GGTNACC 1 cut(s) 3008
BstSFI CTRYAG 4 cut(s) 181, 555, 2225, 2547
BstSLI GKGCMC 4 cut(s) 255, 1047, 1857, 2119
BstUI CGCG 3 cut(s) 59, 2859, 2922
BstV1I GCAGC 4 cut(s) 769, 878, 2073, 2886
BstV2I GAAGAC 4 cut(s) 1748, 2200, 2398, 2889
BstX2I RGATCY 4 cut(s) 177, 1245, 1801, 2613
BstYI RGATCY 4 cut(s) 177, 1245, 1801, 2613
BstZ17I GTATAC 1 cut(s) 1726
BsuI GTATCC 2 cut(s) 228, 1370
BsuRI GGCC 7 cut(s) 137, 845, 1233, 1526, 2246, 2610, 2784
BtgI CCRYGG 1 cut(s) 3280
BtrI CACGTC 1 cut(s) 169
BtsI GCAGTG 1 cut(s) 1095
BtsIMutI CAGTG 6 cut(s) 278, 996, 1095, 1134, 2319, 3253
BveI ACCTGC 2 cut(s) 364, 800
CaiI CAGNNNCTG 2 cut(s) 1522, 2258
CfoI GCGC 4 cut(s) 19, 227, 432, 2859
Cfr13I GGNCC 5 cut(s) 965, 1106, 2245, 2608, 2782
CseI GACGC 5 cut(s) 48, 82, 206, 773, 1668
Csp6I GTAC 5 cut(s) 142, 935, 1626, 2357, 2429
CviQI GTAC 5 cut(s) 142, 935, 1626, 2357, 2429
DinI GGCGCC 1 cut(s) 18
DraIII CACNNNGTG 1 cut(s) 1663
DrdI GACNNNNNNGTC 1 cut(s) 41
DriI GACNNNNNGTC 1 cut(s) 58
DseDI GACNNNNNNGTC 1 cut(s) 41
EaeI YGGCCR 4 cut(s) 135, 843, 1231, 1524
Eam1104I CTCTTC 3 cut(s) 150, 721, 2304
Eam1105I GACNNNNNGTC 1 cut(s) 58
EarI CTCTTC 3 cut(s) 150, 721, 2304
Eco130I CCWWGG 3 cut(s) 1024, 1411, 3280
Eco31I GGTCTC 1 cut(s) 3290
Eco32I GATATC 1 cut(s) 3146
Eco47I GGWCC 2 cut(s) 965, 1106
Eco57I CTGAAG 2 cut(s) 254, 631
Eco91I GGTNACC 1 cut(s) 3008
EcoO109I RGGNCCY 1 cut(s) 965
EcoO65I GGTNACC 1 cut(s) 3008
EcoRII CCWGG 9 cut(s) 190, 255, 733, 1233, 1698, 2188, 2251, 2894, 3047
EcoRV GATATC 1 cut(s) 3146
EcoT14I CCWWGG 3 cut(s) 1024, 1411, 3280
EcoT22I ATGCAT 1 cut(s) 1180
EgeI GGCGCC 1 cut(s) 18
EheI GGCGCC 1 cut(s) 18
ErhI CCWWGG 3 cut(s) 1024, 1411, 3280
Esp3I CGTCTC 1 cut(s) 1686
FaqI GGGAC 5 cut(s) 29, 53, 978, 2306, 3223
FauI CCCGC 2 cut(s) 216, 2721
FblI GTMKAC 2 cut(s) 116, 1725
FspBI CTAG 9 cut(s) 360, 908, 1101, 1397, 1440, 1692, 1937, 2808, 3234
GlaI GCGC 4 cut(s) 18, 226, 431, 2858
GsaI CCCAGC 2 cut(s) 856, 2116
GsuI CTGGAG 4 cut(s) 807, 825, 2574, 2878
HaeII RGCGCY 1 cut(s) 20
HaeIII GGCC 7 cut(s) 137, 845, 1233, 1526, 2246, 2610, 2784
HapII CCGG 5 cut(s) 14, 37, 175, 214, 335
HgaI GACGC 5 cut(s) 48, 82, 206, 773, 1668
HhaI GCGC 4 cut(s) 19, 227, 432, 2859
Hin1I GRCGYC 5 cut(s) 17, 42, 74, 119, 198
Hin6I GCGC 4 cut(s) 17, 225, 430, 2857
HinP1I GCGC 4 cut(s) 17, 225, 430, 2857
HincII GTYRAC 6 cut(s) 117, 682, 1119, 1258, 2955, 3025
HindII GTYRAC 6 cut(s) 117, 682, 1119, 1258, 2955, 3025
HindIII AAGCTT 1 cut(s) 2235
HinfI GANTC 6 cut(s) 53, 1139, 2294, 2336, 2556, 3230
HpaI GTTAAC 1 cut(s) 1258
HpaII CCGG 5 cut(s) 14, 37, 175, 214, 335
HphI GGTGA 7 cut(s) 530, 1290, 1457, 2725, 2827, 2887, 3103
Hpy188III TCNNGA 8 cut(s) 374, 674, 875, 908, 1249, 2617, 2768, 2797
HpyCH4III ACNGT 3 cut(s) 559, 1000, 1123
Hsp92I GRCGYC 5 cut(s) 17, 42, 74, 119, 198
HspAI GCGC 4 cut(s) 17, 225, 430, 2857
KasI GGCGCC 1 cut(s) 16
KspAI GTTAAC 1 cut(s) 1258
LmnI GCTCC 7 cut(s) 216, 462, 623, 788, 806, 1700, 2922
Lsp1109I GCAGC 4 cut(s) 769, 878, 2073, 2886
MaeI CTAG 9 cut(s) 360, 908, 1101, 1397, 1440, 1692, 1937, 2808, 3234
MaeIII GTNAC 6 cut(s) 1189, 1357, 1433, 2205, 2397, 3008
MbiI CCGCTC 2 cut(s) 211, 848
MfeI CAATTG 3 cut(s) 1332, 2046, 2216
MflI RGATCY 4 cut(s) 177, 1245, 1801, 2613
MhlI GDGCHC 9 cut(s) 255, 715, 793, 1047, 1857, 2119, 2577, 2919, 3006
MlsI TGGCCA 1 cut(s) 1233
MluNI TGGCCA 1 cut(s) 1233
Mly113I GGCGCC 1 cut(s) 17
MlyI GAGTC 3 cut(s) 47, 2288, 2565
MmeI TCCRAC 4 cut(s) 1040, 1596, 2143, 2802
Mox20I TGGCCA 1 cut(s) 1233
Mph1103I ATGCAT 1 cut(s) 1180
MscI TGGCCA 1 cut(s) 1233
MslI CAYNNNNRTG 4 cut(s) 288, 657, 1600, 1860
Msp20I TGGCCA 1 cut(s) 1233
MspA1I CMGCKG 1 cut(s) 1808
MspI CCGG 5 cut(s) 14, 37, 175, 214, 335
MunI CAATTG 3 cut(s) 1332, 2046, 2216
Mva1269I GAATGC 5 cut(s) 355, 835, 963, 1180, 2034
MvaI CCWGG 9 cut(s) 192, 257, 735, 1235, 1700, 2190, 2253, 2896, 3049
MvnI CGCG 3 cut(s) 59, 2859, 2922
NarI GGCGCC 1 cut(s) 17
NciI CCSGG 2 cut(s) 14, 38
NcoI CCATGG 1 cut(s) 3280
NheI GCTAGC 1 cut(s) 1691
NlaIV GGNNCC 9 cut(s) 18, 188, 458, 587, 625, 966, 1953, 2733, 3098
NmuCI GTSAC 1 cut(s) 1357
NsiI ATGCAT 1 cut(s) 1180
NspI RCATGY 4 cut(s) 1384, 1732, 1865, 2017
NspV TTCGAA 2 cut(s) 2362, 3240
PaqCI CACCTGC 1 cut(s) 364
PciI ACATGT 1 cut(s) 1380
PcsI WCGNNNNNNNCGW 4 cut(s) 48, 108, 150, 3139
PctI GAATGC 5 cut(s) 355, 835, 963, 1180, 2034
PfeI GAWTC 3 cut(s) 1139, 2336, 3230
PflFI GACNNNGTC 1 cut(s) 76
PfoI TCCNGGA 1 cut(s) 733
PleI GAGTC 3 cut(s) 47, 2288, 2564
PluTI GGCGCC 1 cut(s) 20
PpsI GAGTC 3 cut(s) 47, 2288, 2564
Ppu21I YACGTR 2 cut(s) 659, 3134
PpuMI RGGWCCY 1 cut(s) 965
PscI ACATGT 1 cut(s) 1380
PshAI GACNNNNGTC 1 cut(s) 3282
PshBI ATTAAT 1 cut(s) 1598
Psp5II RGGWCCY 1 cut(s) 965
Psp6I CCWGG 9 cut(s) 190, 255, 733, 1233, 1698, 2188, 2251, 2894, 3047
PspEI GGTNACC 1 cut(s) 3008
PspFI CCCAGC 2 cut(s) 852, 2112
PspGI CCWGG 9 cut(s) 190, 255, 733, 1233, 1698, 2188, 2251, 2894, 3047
PspN4I GGNNCC 9 cut(s) 18, 188, 458, 587, 625, 966, 1953, 2733, 3098
PspPI GGNCC 5 cut(s) 965, 1106, 2245, 2608, 2782
PspPPI RGGWCCY 1 cut(s) 965
PsrI GAACNNNNNNTAC 2 cut(s) 714, 746
PstNI CAGNNNCTG 2 cut(s) 1522, 2258
PsuI RGATCY 4 cut(s) 177, 1245, 1801, 2613
PsyI GACNNNGTC 1 cut(s) 76
PvuII CAGCTG 1 cut(s) 1808
RsaI GTAC 5 cut(s) 143, 936, 1627, 2358, 2430
RsaNI GTAC 5 cut(s) 142, 935, 1626, 2357, 2429
RseI CAYNNNNRTG 4 cut(s) 288, 657, 1600, 1860
SalI GTCGAC 1 cut(s) 115
Sau96I GGNCC 5 cut(s) 965, 1106, 2245, 2608, 2782
SchI GAGTC 3 cut(s) 47, 2288, 2565
SduI GDGCHC 9 cut(s) 255, 715, 793, 1047, 1857, 2119, 2577, 2919, 3006
SfcI CTRYAG 4 cut(s) 181, 555, 2225, 2547
SfoI GGCGCC 1 cut(s) 18
SfuI TTCGAA 2 cut(s) 2362, 3240
SinI GGWCC 2 cut(s) 965, 1106
SmiMI CAYNNNNRTG 4 cut(s) 288, 657, 1600, 1860
SmlI CTYRAG 2 cut(s) 875, 2795
SmoI CTYRAG 2 cut(s) 875, 2795
SspDI GGCGCC 1 cut(s) 16
SspI AATATT 2 cut(s) 1038, 2302
SspMI CTAG 9 cut(s) 360, 908, 1101, 1397, 1440, 1692, 1937, 2808, 3234
StyI CCWWGG 3 cut(s) 1024, 1411, 3280
TaaI ACNGT 3 cut(s) 559, 1000, 1123
TaqI TCGA 5 cut(s) 116, 1762, 2297, 2362, 3240
TaqII GACCGA 2 cut(s) 3156, 3282
TatI WGTACW 2 cut(s) 1625, 2356
TauI GCSGC 8 cut(s) 22, 25, 131, 134, 137, 496, 848, 1529
TfiI GAWTC 3 cut(s) 1139, 2336, 3230
TscAI CASTG 6 cut(s) 278, 1003, 1102, 1141, 2326, 3253
TseFI GTSAC 1 cut(s) 1357
TseI GCWGC 4 cut(s) 782, 866, 2086, 2899
Tsp45I GTSAC 1 cut(s) 1357
TspGWI ACGGA 8 cut(s) 52, 210, 439, 1421, 1820, 1873, 2190, 3278
TspRI CASTG 6 cut(s) 278, 1003, 1102, 1141, 2326, 3253
Tth111I GACNNNGTC 1 cut(s) 76
VneI GTGCAC 2 cut(s) 251, 1853
VpaK11BI GGWCC 2 cut(s) 965, 1106
VspI ATTAAT 1 cut(s) 1598
XapI RAATTY 2 cut(s) 1195, 2001
XbaI TCTAGA 1 cut(s) 907
XceI RCATGY 4 cut(s) 1384, 1732, 1865, 2017
XmiI GTMKAC 2 cut(s) 116, 1725
XspI CTAG 9 cut(s) 360, 908, 1101, 1397, 1440, 1692, 1937, 2808, 3234
ZraI GACGTC 2 cut(s) 43, 120
Zsp2I ATGCAT 1 cut(s) 1180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.