MD05G1085000.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009109
Physical Location & Seq
Forward (+)
17741640 .. 17747268
5629 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1085000.v1.1.491

Sequence Viewer

Length: 3333 bp
ATGGAGATCGCCCCGGCCCCGCCGCCGTCCGCTGACCGGGACATCCCAATCGATACCGGCAAGACGTCGTCGTCTCAGGAGAACGAAGCGAGCGCCGCGGCGGCGGCGGCGACGACGACCTTGTCTTCGTCGGCGTCGTCGGTGGCGGTCAAGTACGACGATGAGGAAGAGGAGGAGGACGTGTGCCGGATCTGTAGAAACCCGGGCGACGCCGATAACCCGCTCCGGTACCCGTGCGCTTGTAGCGGAAGCATCAAATTTGTGCACCAGGATTGCCTCCTGCAGTGGCTCAATCACAGCAATGCTCGCCAATGCGAGGTTTGCAAGCATGCATTTTCCTTCTCTCCAGTTTATGCTGAGAATGCCCCTGCAAGGCTTCCTTTTCAAGAGTTTGTAGTTGGGATGGCAATGAAAACTTGCCATGTTCTGCAATTCTTTCTGCGCCTTAGTTTTGTGCTTTCTGTTTGGCTCCTCATTATACCTTTTATTACATTTTGGATATGGCGGTTGGCTTTTGTGAGGAGTTTTGGTGAAGCTCAGAGATTATTCCTTAGTCATTTATCCACTACAGTAATCCTTACCGATTGTCTACATGGGTTCCTACTTTCTGCTAGCATTGTGTTTATTTTTCTCGGGGCCACTTCTCTGAGGGATTACTTCAGGCATTTACGTGAGCTAGGAGGACAGGATGCTGACAGAGAAGATGAAGGGGAAAGAAATGGTGCCCGTGCTGCAAGGAGAGCTCCTGGACAAGCTAACAGGAACTTTGTTGGTGATGTGAATGGGGAAGATGCTGCCGGAGCACAAGGGATTGCCGGAGCAGGTCAAATGATTAGAAGGAATGCAGAAAATGTTGCTGCTCGGTGGGAGATGCAGGCAGCTCGTCTTGAAGCTCATGTGGAACAGATGTTTGATGGTCTGGATGATGCTGATGGCGCCGAGGATGTACCGTTTGATGAGCTCGTTGGCATGCAGGGACCTGTATTTCATTTAGTTGAAAATGCATTCACTGTTCTGGCCAGCAATATGATATTCCTTGGTGTAGTAATCTTTGTGCCTTTCTCAATTGGTCGGATTATACTCTATCATTTGTCTTGGCTTTTCTCCACTGCTACTCGTCCGGTATTGTCCACGGTCATGCCACTCACAGAATCAGCCCTTTCCTTGGCAAATGTGACATTGAAGAATGCATTAACAGCCGTAACGAATGTATCATCTGAAAGCCAACAAAGTGGTATGGTTGGCCAGGTTGAAGAGACCTTGAAAGCAAACATGAGTGGACTAAATGAGGTAGCAAACAATATAAGTTCACCACTTTCAGCAGACTTCCTGAAAGGGGCAACACTTGGGACATCAAGGCTGTCTGATGTTACAACTCTTGCTATTGGATACATGTTTATATTCTCCCTAGTTTTCTTCTACCTTGGCATTGTTGCTTTGATTCGGTACACTAGGGGTGAGCCATTGACTTTGGGAAGGTTCTACGGTATTGCTTCTATGGCAGAGACAATTCCATCTCTCTTCAGGCAGTTCTTGGCAGCAATGAGGCATTTGATGACTATGATTAAGGTTGCATTTCTTCTAGTCATTGAACTGGGGGTATTTCCATTGATGTGTGGATGGTGGCTAGATGTTTGCACAATAAGGATGTTTGGGAAGTCCATGTCTCACAGGGTTCAATTCTTCTCAGCATCTCCCTTAGCCAGTTCACTGGTCCATTGGGTTGTTGGAATTGTATACATGCTACAAATAAGCATATTCGTCAGTCTTCTTCGAGGGGTTCTGCGTAATGGAGTTCTTTATTTCCTTAGAGATCCAGCTGATCCAAACTACAATCCCTTCCGCGATCTTATTGATGATCCGGTGCACAAACATGCCCGCAGGGTCCTGTTATCTGTTGCGGTGTATGGGAGTTTAATTGTGATGCTGGTGTTTTTACCGGTTAAACTAGCTATGCGGATGGCACCTTCCATTTTTCCTCTTGACATCTCGGTGTCGGACCCATTTACTGAAATTCCTGCGGACATGCTTCTTTTTCAAATATGCATTCCATTCGCCATTGAGCATTTCAAATTACGGACAACGATTAAATCCCTCCTCCGTTATTGGTTTACGGCAGTTGGTTGGGCACTTGGTTTAACGGAATTCTTACTTCCCAGACCTGAGGACAATGGTGCTCAGGAAAATGGAAATGCAGAGCCAGGTAGACAGGATAGAGTACAAGTTCAACTAGGGGTACATGATCAGGCTTTGGTGGCACTTCCAGGTGCTGATGATCCTAATGCGGGCATTCTTGCATCAGGGGACTCAATTGTCACAGAAGAGTATGACACAGATGAACAATCTGATTCTGAAAGGTACAGTTTTGTGCTTCGCATTGTACTCTTGTTGGTGGTGGCTTGGATGACGTTACTTGTCTTCAACTCTGCCTTAATAGTCGTACCAACCTCACTTGGAAGGGCAATTTTCAATGTCATTCCTTTTCTCCCTATAACACATGGAATCAAGTGCAATGATTTGTATGCTTTCATCATTGGAAGCTACATAATATGGACTGCTGTGGCCGGAGTAAGATACTCAATTGAGCATATTCGAACAAAGAGGGTTGCAGTTCTGTTAGGCCAGATCTGGAAGTGGTGTGCCATTGTTATCAAGAGTTCCGCACTTTTGTCAATATGGATTTTTGTCATTCCGGTATTGATTGGGCTGCTCTTTGAGCTTCTGGTGATCGTGCCGATGCGTGTGCCTGTTGATGAAAGCCCAGTGTTCTTGCTTTACCAGGACTGGGCATTGGGCCTTATTTTTCTCAAGATATGGACTAGACTGGTTATGTTGGATCACATGATGCCACTGGTGGATGAAACATGGCGAGTAAAGTTCGAAAGGGTGAGGGAAGACGGTTTCTCTAGGCTGCAAGGTCTTTGGGTGCTTCGCGAGATTGTATTCCCAATCATAATGAAGTTGTTGACTGCCCTGTGTGTGCCTTACGTACTTGCCAGGGGGCTATTTCCTGTTCTCGGTTACCCATTAGTGGTGAATTCAGCAGTCTATCGGTTTGCCTGGCTGGGATGCCTTTGCTTTAGCCTGTTGTGCTTTTGCGCCAAGAGATTCCATGTCTGGTTCACCAACCTTCACAACTCAATACGTGACGACCGGTATCTAGTTGGTCGTAGGCTTCATAACTTTGGAGAAGCAATCGAAGAGAAGCAAAACGAAGCAGGGACATCCTCGGAAGTGCAGGGCTCTAACTTCGAAACCAACGGGCTAATCCGGTACGACCGAGAAGTTGATATAGGACTCCGGCTAAGGCACGTTAACCGAGTTGATGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000209 GO:0001101 GO:0002020 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006508 GO:0006511 GO:0006629 GO:0006720 GO:0006725 GO:0006807 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009414 GO:0009415 GO:0009628 GO:0009698 GO:0009699 GO:0009893 GO:0009987 GO:0010025 GO:0010033 GO:0010035 GO:0010143 GO:0010166 GO:0010243 GO:0010345 GO:0010498 GO:0012505 GO:0016020 GO:0016021 GO:0016567 GO:0016740 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019787 GO:0019899 GO:0019941 GO:0030163 GO:0030176 GO:0030433 GO:0031224 GO:0031227 GO:0031624 GO:0031984 GO:0032446 GO:0032501 GO:0032502 GO:0033554 GO:0034976 GO:0036211 GO:0036503 GO:0042175 GO:0042221 GO:0042335 GO:0043085 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044390 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0048518 GO:0048856 GO:0050789 GO:0050790 GO:0050896 GO:0051341 GO:0051353 GO:0051603 GO:0051716 GO:0065007 GO:0065009 GO:0070647 GO:0070936 GO:0071704 GO:0098827 GO:0140096 GO:1900490 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901568 GO:1901570 GO:1901575 GO:1901576 GO:1901698 GO:1901700 GO:1990381 GO:2001215
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
RINGv

Protein Analysis

1111

Amino Acids

123.97

Weight (kDa)

5.88

Isoelectric Point (pI)

38.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 62 - 108 9.9e-17 RING-variant domain
MARCHF6_C PF23113 871 - 1049 1.4e-50 E3 ubiquitin-protein ligase MARCHF6-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 70, 121, 2312
AatII GACGTC 1 cut(s) 68
Acc36I ACCTGC 1 cut(s) 812
Acc65I GGTACC 1 cut(s) 228
AccB1I GGYRCC 4 cut(s) 228, 722, 935, 1963
AccBSI CCGCTC 1 cut(s) 223
AccI GTMKAC 3 cut(s) 589, 1737, 2206
AccII CGCG 3 cut(s) 98, 1845, 2934
AclWI GGATC 6 cut(s) 197, 1808, 1817, 1853, 2270, 2844
AcoI YGGCCR 3 cut(s) 1017, 1243, 2562
AcsI RAATTY 4 cut(s) 257, 2013, 2144, 3037
AcuI CTGAAG 2 cut(s) 643, 1507
AcyI GRCGYC 4 cut(s) 65, 134, 210, 936
AfiI CCNNNNNNNGG 9 cut(s) 36, 316, 372, 1165, 1336, 2285, 2785, 3017, 3031
AflIII ACRYGT 2 cut(s) 180, 1392
AgeI ACCGGT 2 cut(s) 1939, 3153
AjiI CACGTC 1 cut(s) 181
AjnI CCWGG 8 cut(s) 267, 745, 1245, 2200, 2263, 2778, 2996, 3059
Alw21I GWGCWC 6 cut(s) 267, 745, 805, 963, 1869, 2179
Alw26I GTCTC 4 cut(s) 78, 1250, 1499, 1671
Alw44I GTGCAC 2 cut(s) 263, 1865
AlwI GGATC 6 cut(s) 197, 1808, 1817, 1853, 2270, 2844
AlwNI CAGNNNCTG 1 cut(s) 2270
Ama87I CYCGRG 2 cut(s) 202, 632
AoxI GGCC 7 cut(s) 15, 636, 1017, 1243, 2562, 2620, 2794
ApaLI GTGCAC 2 cut(s) 263, 1865
ApeKI GCWGC 7 cut(s) 731, 794, 857, 878, 1538, 2707, 2911
ApoI RAATTY 4 cut(s) 257, 2013, 2144, 3037
ArsI GACNNNNNNTTYG 2 cut(s) 109, 141
AsiGI ACCGGT 2 cut(s) 1939, 3153
Asp718I GGTACC 1 cut(s) 228
AspLEI GCGC 5 cut(s) 95, 239, 444, 938, 3101
AspS9I GGNCC 7 cut(s) 16, 636, 977, 1714, 1885, 1999, 2794
AsuC2I CCSGG 4 cut(s) 14, 38, 203, 204
AsuHPI GGTGA 8 cut(s) 542, 785, 1302, 1469, 2737, 2899, 3046, 3115
AsuII TTCGAA 3 cut(s) 2593, 2880, 3252
AsuNHI GCTAGC 1 cut(s) 611
AvaI CYCGRG 2 cut(s) 202, 632
AvaII GGWCC 4 cut(s) 977, 1714, 1885, 1999
AxyI CCTNAGG 1 cut(s) 2163
BaeGI GKGCMC 4 cut(s) 267, 727, 1869, 2131
BaeI ACNNNNGTAYC 2 cut(s) 1194, 1227
BalI TGGCCA 2 cut(s) 1019, 1245
BanI GGYRCC 4 cut(s) 228, 722, 935, 1963
BanII GRGCYC 3 cut(s) 745, 963, 3245
BbsI GAAGAC 4 cut(s) 117, 1760, 2410, 2901
Bbv12I GWGCWC 6 cut(s) 267, 745, 805, 963, 1869, 2179
BbvI GCAGC 7 cut(s) 718, 781, 844, 890, 1550, 2694, 2898
BccI CCATC 6 cut(s) 397, 908, 926, 1522, 1614, 1954
BceAI ACGGC 3 cut(s) 10, 1184, 2130
BcgI CGANNNNNNTGC 6 cut(s) 863, 897, 2035, 2069, 2725, 2759
BciT130I CCWGG 8 cut(s) 269, 747, 1247, 2202, 2265, 2780, 2998, 3061
BciVI GTATCC 1 cut(s) 1382
BclI TGATCA 1 cut(s) 2242
BcnI CCSGG 4 cut(s) 14, 38, 203, 204
BcoDI GTCTC 4 cut(s) 78, 1250, 1499, 1671
BfmI CTRYAG 3 cut(s) 193, 281, 567
BfoI RGCGCY 2 cut(s) 96, 939
BfuAI ACCTGC 1 cut(s) 812
BfuI GTATCC 1 cut(s) 1382
BglI GCCNNNNNGGC 1 cut(s) 101
BglII AGATCT 1 cut(s) 2625
Bme18I GGWCC 4 cut(s) 977, 1714, 1885, 1999
BmeT110I CYCGRG 2 cut(s) 202, 632
BmgBI CACGTC 1 cut(s) 181
BmgT120I GGNCC 7 cut(s) 16, 636, 977, 1714, 1885, 1999, 2794
BmrI ACTGGG 3 cut(s) 1604, 2756, 2794
BmtI GCTAGC 1 cut(s) 615
BmuI ACTGGG 3 cut(s) 1604, 2756, 2794
BpiI GAAGAC 4 cut(s) 117, 1760, 2410, 2901
BpmI CTGGAG 1 cut(s) 330
Bpu10I CCTNAGC 3 cut(s) 1699, 2178, 3305
Bpu14I TTCGAA 3 cut(s) 2593, 2880, 3252
BpuEI CTTGAG 1 cut(s) 2792
BpuMI CCSGG 4 cut(s) 14, 38, 203, 204
Bsa29I ATCGAT 1 cut(s) 51
BsaAI YACGTR 3 cut(s) 671, 2989, 3146
BsaHI GRCGYC 4 cut(s) 65, 134, 210, 936
BsaI GGTCTC 1 cut(s) 1250
BsaWI WCCGGW 7 cut(s) 225, 1120, 1861, 1939, 2692, 3153, 3270
BsaXI ACNNNNNCTCC 4 cut(s) 167, 197, 514, 544
Bsc4I CCNNNNNNNGG 9 cut(s) 36, 316, 372, 1165, 1336, 2285, 2785, 3017, 3031
Bse118I RCCGGY 3 cut(s) 56, 1939, 3153
Bse1I ACTGG 8 cut(s) 347, 1599, 1704, 1716, 2762, 2789, 2829, 2856
Bse21I CCTNAGG 1 cut(s) 2163
Bse3DI GCAATG 4 cut(s) 307, 414, 1548, 2518
BseBI CCWGG 8 cut(s) 269, 747, 1247, 2202, 2265, 2780, 2998, 3061
BseCI ATCGAT 1 cut(s) 51
BseLI CCNNNNNNNGG 9 cut(s) 36, 316, 372, 1165, 1336, 2285, 2785, 3017, 3031
BseMI GCAATG 4 cut(s) 307, 414, 1548, 2518
BseMII CTCAG 7 cut(s) 89, 348, 551, 638, 1701, 2154, 2192
BseNI ACTGG 8 cut(s) 347, 1599, 1704, 1716, 2762, 2789, 2829, 2856
BseRI GAGGAG 5 cut(s) 185, 188, 461, 535, 2087
BseSI GKGCMC 4 cut(s) 267, 727, 1869, 2131
BseXI GCAGC 7 cut(s) 718, 781, 844, 890, 1550, 2694, 2898
BseYI CCCAGC 1 cut(s) 3064
BsgI GTGCAG 1 cut(s) 3257
Bsh1236I CGCG 3 cut(s) 98, 1845, 2934
Bsh1285I CGRYCG 2 cut(s) 3154, 3280
BshFI GGCC 7 cut(s) 17, 638, 1019, 1245, 2564, 2622, 2796
BshNI GGYRCC 4 cut(s) 228, 722, 935, 1963
BshTI ACCGGT 2 cut(s) 1939, 3153
BshVI ATCGAT 1 cut(s) 51
BsiEI CGRYCG 2 cut(s) 3154, 3280
BsiHKAI GWGCWC 6 cut(s) 267, 745, 805, 963, 1869, 2179
BsiHKCI CYCGRG 2 cut(s) 202, 632
BslFI GGGAC 5 cut(s) 53, 990, 1363, 2318, 3235
BslI CCNNNNNNNGG 9 cut(s) 36, 316, 372, 1165, 1336, 2285, 2785, 3017, 3031
BsmAI GTCTC 4 cut(s) 78, 1250, 1499, 1671
BsmBI CGTCTC 1 cut(s) 78
BsmFI GGGAC 5 cut(s) 53, 990, 1363, 2318, 3235
BsmI GAATGC 6 cut(s) 367, 847, 1004, 1192, 2046, 2289
BsnI GGCC 7 cut(s) 17, 638, 1019, 1245, 2564, 2622, 2796
Bso31I GGTCTC 1 cut(s) 1250
BsoBI CYCGRG 2 cut(s) 202, 632
Bsp119I TTCGAA 3 cut(s) 2593, 2880, 3252
Bsp1286I GDGCHC 9 cut(s) 267, 727, 745, 805, 963, 1869, 2131, 2179, 3245
Bsp68I TCGCGA 1 cut(s) 2934
BspANI GGCC 7 cut(s) 17, 638, 1019, 1245, 2564, 2622, 2796
BspCNI CTCAG 7 cut(s) 88, 349, 550, 639, 1700, 2155, 2191
BspDI ATCGAT 1 cut(s) 51
BspFNI CGCG 3 cut(s) 98, 1845, 2934
BspMAI CTGCAG 1 cut(s) 285
BspMI ACCTGC 1 cut(s) 812
BspOI GCTAGC 1 cut(s) 615
BspPI GGATC 6 cut(s) 197, 1808, 1817, 1853, 2270, 2844
BspT104I TTCGAA 3 cut(s) 2593, 2880, 3252
BspT107I GGYRCC 4 cut(s) 228, 722, 935, 1963
BspTNI GGTCTC 1 cut(s) 1250
BsrBI CCGCTC 1 cut(s) 223
BsrDI GCAATG 4 cut(s) 307, 414, 1548, 2518
BsrFI RCCGGY 3 cut(s) 56, 1939, 3153
BsrI ACTGG 8 cut(s) 347, 1599, 1704, 1716, 2762, 2789, 2829, 2856
BssAI RCCGGY 3 cut(s) 56, 1939, 3153
BssNAI GTATAC 1 cut(s) 1738
BssNI GRCGYC 4 cut(s) 65, 134, 210, 936
BssT1I CCWWGG 3 cut(s) 1036, 1164, 1423
Bst1107I GTATAC 1 cut(s) 1738
Bst2UI CCWGG 8 cut(s) 269, 747, 1247, 2202, 2265, 2780, 2998, 3061
Bst4CI ACNGT 7 cut(s) 571, 951, 1012, 1135, 1487, 2363, 2900
Bst6I CTCTTC 5 cut(s) 162, 1248, 1526, 2316, 3195
BstACI GRCGYC 4 cut(s) 65, 134, 210, 936
BstBAI YACGTR 3 cut(s) 671, 2989, 3146
BstBI TTCGAA 3 cut(s) 2593, 2880, 3252
BstDSI CCRYGG 2 cut(s) 96, 1131
BstEII GGTNACC 1 cut(s) 3020
BstFNI CGCG 3 cut(s) 98, 1845, 2934
BstH2I RGCGCY 2 cut(s) 96, 939
BstHHI GCGC 5 cut(s) 95, 239, 444, 938, 3101
BstMAI GTCTC 4 cut(s) 78, 1250, 1499, 1671
BstMCI CGRYCG 2 cut(s) 3154, 3280
BstNI CCWGG 8 cut(s) 269, 747, 1247, 2202, 2265, 2780, 2998, 3061
BstNSI RCATGY 6 cut(s) 332, 973, 1396, 1744, 1877, 2029
BstPI GGTNACC 1 cut(s) 3020
BstSFI CTRYAG 3 cut(s) 193, 281, 567
BstSLI GKGCMC 4 cut(s) 267, 727, 1869, 2131
BstSNI TACGTA 1 cut(s) 2989
BstUI CGCG 3 cut(s) 98, 1845, 2934
BstV1I GCAGC 7 cut(s) 718, 781, 844, 890, 1550, 2694, 2898
BstV2I GAAGAC 4 cut(s) 117, 1760, 2410, 2901
BstX2I RGATCY 3 cut(s) 189, 1813, 2625
BstXI CCANNNNNNTGG 2 cut(s) 1232, 1711
BstYI RGATCY 3 cut(s) 189, 1813, 2625
BstZ17I GTATAC 1 cut(s) 1738
Bsu15I ATCGAT 1 cut(s) 51
Bsu36I CCTNAGG 1 cut(s) 2163
BsuI GTATCC 1 cut(s) 1382
BsuRI GGCC 7 cut(s) 17, 638, 1019, 1245, 2564, 2622, 2796
BsuTUI ATCGAT 1 cut(s) 51
BtgI CCRYGG 2 cut(s) 96, 1131
BtrI CACGTC 1 cut(s) 181
BtsI GCAGTG 2 cut(s) 290, 1107
BtsIMutI CAGTG 6 cut(s) 290, 1008, 1107, 1709, 2769, 2849
BtuMI TCGCGA 1 cut(s) 2934
BveI ACCTGC 1 cut(s) 812
CaiI CAGNNNCTG 1 cut(s) 2270
CfoI GCGC 5 cut(s) 95, 239, 444, 938, 3101
Cfr10I RCCGGY 3 cut(s) 56, 1939, 3153
Cfr13I GGNCC 7 cut(s) 16, 636, 977, 1714, 1885, 1999, 2794
Cfr42I CCGCGG 1 cut(s) 99
Cfr9I CCCGGG 1 cut(s) 202
ClaI ATCGAT 1 cut(s) 51
CseI GACGC 2 cut(s) 123, 218
CspAI ACCGGT 2 cut(s) 1939, 3153
DinI GGCGCC 1 cut(s) 937
DrdI GACNNNNNNGTC 3 cut(s) 70, 121, 2312
DseDI GACNNNNNNGTC 3 cut(s) 70, 121, 2312
EaeI YGGCCR 3 cut(s) 1017, 1243, 2562
Eam1104I CTCTTC 5 cut(s) 162, 1248, 1526, 2316, 3195
EarI CTCTTC 5 cut(s) 162, 1248, 1526, 2316, 3195
Ecl136II GAGCTC 2 cut(s) 743, 961
Eco105I TACGTA 1 cut(s) 2989
Eco130I CCWWGG 3 cut(s) 1036, 1164, 1423
Eco24I GRGCYC 3 cut(s) 745, 963, 3245
Eco31I GGTCTC 1 cut(s) 1250
Eco47I GGWCC 4 cut(s) 977, 1714, 1885, 1999
Eco53kI GAGCTC 2 cut(s) 743, 961
Eco57I CTGAAG 2 cut(s) 643, 1507
Eco81I CCTNAGG 1 cut(s) 2163
Eco88I CYCGRG 2 cut(s) 202, 632
Eco91I GGTNACC 1 cut(s) 3020
EcoICRI GAGCTC 2 cut(s) 743, 961
EcoO109I RGGNCCY 2 cut(s) 977, 1885
EcoO65I GGTNACC 1 cut(s) 3020
EcoRI GAATTC 2 cut(s) 2144, 3037
EcoRII CCWGG 8 cut(s) 267, 745, 1245, 2200, 2263, 2778, 2996, 3059
EcoT14I CCWWGG 3 cut(s) 1036, 1164, 1423
EcoT22I ATGCAT 4 cut(s) 334, 1006, 1192, 2048
EcoT38I GRGCYC 3 cut(s) 745, 963, 3245
EgeI GGCGCC 1 cut(s) 937
EheI GGCGCC 1 cut(s) 937
ErhI CCWWGG 3 cut(s) 1036, 1164, 1423
Esp3I CGTCTC 1 cut(s) 78
FalI AAGNNNNNCTT 2 cut(s) 364, 396
FaqI GGGAC 5 cut(s) 53, 990, 1363, 2318, 3235
FauI CCCGC 4 cut(s) 27, 228, 1886, 2278
FbaI TGATCA 1 cut(s) 2242
FblI GTMKAC 3 cut(s) 589, 1737, 2206
FriOI GRGCYC 3 cut(s) 745, 963, 3245
GlaI GCGC 5 cut(s) 94, 238, 443, 937, 3100
GsaI CCCAGC 1 cut(s) 3068
GsuI CTGGAG 1 cut(s) 330
HaeII RGCGCY 2 cut(s) 96, 939
HaeIII GGCC 7 cut(s) 17, 638, 1019, 1245, 2564, 2622, 2796
HgaI GACGC 2 cut(s) 123, 218
HhaI GCGC 5 cut(s) 95, 239, 444, 938, 3101
Hin1I GRCGYC 4 cut(s) 65, 134, 210, 936
Hin6I GCGC 5 cut(s) 93, 237, 442, 936, 3099
HinP1I GCGC 5 cut(s) 93, 237, 442, 936, 3099
HincII GTYRAC 2 cut(s) 2967, 3316
HindII GTYRAC 2 cut(s) 2967, 3316
HinfI GANTC 7 cut(s) 1151, 1441, 2306, 2348, 2502, 3108, 3297
HpaI GTTAAC 1 cut(s) 3316
HphI GGTGA 8 cut(s) 542, 785, 1302, 1469, 2737, 2899, 3046, 3115
Hpy188I TCNGA 9 cut(s) 540, 648, 1074, 1219, 1366, 1999, 2347, 2353, 3232
Hpy99I CGWCG 9 cut(s) 70, 73, 115, 118, 133, 139, 142, 161, 212
HpyAV CCTTC 8 cut(s) 349, 701, 831, 1470, 1849, 1977, 2451, 3140
HpyCH4III ACNGT 7 cut(s) 571, 951, 1012, 1135, 1487, 2363, 2900
HpyCH4IV ACGT 7 cut(s) 65, 180, 670, 2408, 2988, 3145, 3312
HpySE526I ACGT 7 cut(s) 65, 180, 670, 2408, 2988, 3145, 3312
Hsp92I GRCGYC 4 cut(s) 65, 134, 210, 936
HspAI GCGC 5 cut(s) 93, 237, 442, 936, 3099
KasI GGCGCC 1 cut(s) 935
KpnI GGTACC 1 cut(s) 232
Ksp22I TGATCA 1 cut(s) 2242
KspAI GTTAAC 1 cut(s) 3316
KspI CCGCGG 1 cut(s) 99
LmnI GCTCC 5 cut(s) 228, 474, 748, 800, 818
Lsp1109I GCAGC 7 cut(s) 718, 781, 844, 890, 1550, 2694, 2898
MaeII ACGT 7 cut(s) 65, 180, 670, 2408, 2988, 3145, 3312
MaeIII GTNAC 7 cut(s) 1174, 1201, 1369, 2314, 2409, 3020, 3146
MbiI CCGCTC 1 cut(s) 223
MfeI CAATTG 3 cut(s) 1065, 2310, 2580
MflI RGATCY 3 cut(s) 189, 1813, 2625
MhlI GDGCHC 9 cut(s) 267, 727, 745, 805, 963, 1869, 2131, 2179, 3245
MlsI TGGCCA 2 cut(s) 1019, 1245
MluNI TGGCCA 2 cut(s) 1019, 1245
Mly113I GGCGCC 1 cut(s) 936
MlyI GAGTC 2 cut(s) 2300, 3291
MmeI TCCRAC 4 cut(s) 1052, 1708, 1977, 2814
Mox20I TGGCCA 2 cut(s) 1019, 1245
Mph1103I ATGCAT 4 cut(s) 334, 1006, 1192, 2048
MscI TGGCCA 2 cut(s) 1019, 1245
MseI TTAA 8 cut(s) 1193, 1566, 1916, 1944, 2088, 2138, 2432, 3315
MslI CAYNNNNRTG 6 cut(s) 300, 669, 1136, 1612, 1872, 1991
Msp20I TGGCCA 2 cut(s) 1019, 1245
MspA1I CMGCKG 3 cut(s) 32, 98, 1820
MunI CAATTG 3 cut(s) 1065, 2310, 2580
Mva1269I GAATGC 6 cut(s) 367, 847, 1004, 1192, 2046, 2289
MvaI CCWGG 8 cut(s) 269, 747, 1247, 2202, 2265, 2780, 2998, 3061
MvnI CGCG 3 cut(s) 98, 1845, 2934
NarI GGCGCC 1 cut(s) 936
NciI CCSGG 4 cut(s) 14, 38, 203, 204
NheI GCTAGC 1 cut(s) 611
NmeAIII GCCGAG 1 cut(s) 964
NmuCI GTSAC 3 cut(s) 1174, 2314, 3146
NruI TCGCGA 1 cut(s) 2934
NsiI ATGCAT 4 cut(s) 334, 1006, 1192, 2048
NspI RCATGY 6 cut(s) 332, 973, 1396, 1744, 1877, 2029
NspV TTCGAA 3 cut(s) 2593, 2880, 3252
PaeI GCATGC 2 cut(s) 332, 973
PciI ACATGT 1 cut(s) 1392
PcsI WCGNNNNNNNCGW 1 cut(s) 134
PctI GAATGC 6 cut(s) 367, 847, 1004, 1192, 2046, 2289
PfeI GAWTC 5 cut(s) 1151, 1441, 2348, 2502, 3108
PflFI GACNNNGTC 2 cut(s) 67, 121
PfoI TCCNGGA 1 cut(s) 745
PinAI ACCGGT 2 cut(s) 1939, 3153
PleI GAGTC 2 cut(s) 2300, 3291
PluTI GGCGCC 1 cut(s) 939
PpsI GAGTC 2 cut(s) 2300, 3291
Ppu21I YACGTR 3 cut(s) 671, 2989, 3146
PpuMI RGGWCCY 2 cut(s) 977, 1885
PscI ACATGT 1 cut(s) 1392
Psp124BI GAGCTC 2 cut(s) 745, 963
Psp5II RGGWCCY 2 cut(s) 977, 1885
Psp6I CCWGG 8 cut(s) 267, 745, 1245, 2200, 2263, 2778, 2996, 3059
PspEI GGTNACC 1 cut(s) 3020
PspFI CCCAGC 1 cut(s) 3064
PspGI CCWGG 8 cut(s) 267, 745, 1245, 2200, 2263, 2778, 2996, 3059
PspPI GGNCC 7 cut(s) 16, 636, 977, 1714, 1885, 1999, 2794
PspPPI RGGWCCY 2 cut(s) 977, 1885
PstI CTGCAG 1 cut(s) 285
PstNI CAGNNNCTG 1 cut(s) 2270
PsuI RGATCY 3 cut(s) 189, 1813, 2625
PsyI GACNNNGTC 2 cut(s) 67, 121
PvuII CAGCTG 1 cut(s) 1820
RruI TCGCGA 1 cut(s) 2934
RseI CAYNNNNRTG 6 cut(s) 300, 669, 1136, 1612, 1872, 1991
SacI GAGCTC 2 cut(s) 745, 963
SacII CCGCGG 1 cut(s) 99
SaqAI TTAA 8 cut(s) 1193, 1566, 1916, 1944, 2088, 2138, 2432, 3315
Sau96I GGNCC 7 cut(s) 16, 636, 977, 1714, 1885, 1999, 2794
SchI GAGTC 2 cut(s) 2300, 3291
SduI GDGCHC 9 cut(s) 267, 727, 745, 805, 963, 1869, 2131, 2179, 3245
SfcI CTRYAG 3 cut(s) 193, 281, 567
SfoI GGCGCC 1 cut(s) 937
Sfr303I CCGCGG 1 cut(s) 99
SfuI TTCGAA 3 cut(s) 2593, 2880, 3252
SgrBI CCGCGG 1 cut(s) 99
SinI GGWCC 4 cut(s) 977, 1714, 1885, 1999
SmaI CCCGGG 1 cut(s) 204
SmiMI CAYNNNNRTG 6 cut(s) 300, 669, 1136, 1612, 1872, 1991
SmlI CTYRAG 1 cut(s) 2807
SmoI CTYRAG 1 cut(s) 2807
SnaBI TACGTA 1 cut(s) 2989
SphI GCATGC 2 cut(s) 332, 973
SspDI GGCGCC 1 cut(s) 935
SstI GAGCTC 2 cut(s) 745, 963
StyI CCWWGG 3 cut(s) 1036, 1164, 1423
TaaI ACNGT 7 cut(s) 571, 951, 1012, 1135, 1487, 2363, 2900
TaiI ACGT 7 cut(s) 68, 183, 673, 2411, 2991, 3148, 3315
TaqI TCGA 6 cut(s) 51, 1774, 2593, 2880, 3198, 3252
TaqII GACCGA 1 cut(s) 3294
TatI WGTACW 2 cut(s) 2218, 2380
TauI GCSGC 6 cut(s) 25, 98, 101, 104, 107, 110
TfiI GAWTC 5 cut(s) 1151, 1441, 2348, 2502, 3108
Tru1I TTAA 8 cut(s) 1193, 1566, 1916, 1944, 2088, 2138, 2432, 3315
Tru9I TTAA 8 cut(s) 1193, 1566, 1916, 1944, 2088, 2138, 2432, 3315
TscAI CASTG 6 cut(s) 290, 1015, 1114, 1716, 2769, 2856
TseFI GTSAC 3 cut(s) 1174, 2314, 3146
TseI GCWGC 7 cut(s) 731, 794, 857, 878, 1538, 2707, 2911
Tsp45I GTSAC 3 cut(s) 1174, 2314, 3146
TspDTI ATGAA 9 cut(s) 425, 720, 977, 2352, 2518, 2769, 2874, 2972, 3167
TspGWI ACGGA 3 cut(s) 2090, 2092, 2156
TspMI CCCGGG 1 cut(s) 202
TspRI CASTG 6 cut(s) 290, 1015, 1114, 1716, 2769, 2856
Tth111I GACNNNGTC 2 cut(s) 67, 121
VneI GTGCAC 2 cut(s) 263, 1865
VpaK11BI GGWCC 4 cut(s) 977, 1714, 1885, 1999
XapI RAATTY 4 cut(s) 257, 2013, 2144, 3037
XceI RCATGY 6 cut(s) 332, 973, 1396, 1744, 1877, 2029
XcmI CCANNNNNNNNNTGG 1 cut(s) 1724
XmaI CCCGGG 1 cut(s) 202
XmiI GTMKAC 3 cut(s) 589, 1737, 2206
ZraI GACGTC 1 cut(s) 66
Zsp2I ATGCAT 4 cut(s) 334, 1006, 1192, 2048
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.