Rmu_sc0024605.1_g000001

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0024605.1
OG0009109
Physical Location & Seq
Reverse (-)
745 .. 4993
4249 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0024605.1_g000001.1.cds

Sequence Viewer

Length: 3051 bp
atgcacctgattagcttgatatttgcttcgattccgttagccataactttggtttgcaagcatccattttccttctctcccgtttatgctgagaatgcacctgctaggcttccttttcaagagtttgtggttgggatggcaatgaaaacttgtcatgttctgcaatttttcctgcgccttagttttgtgctttccgtttggctcctcattatacctttcatcacattttggatatggcggctggctttcgtgaggagttttggtgaagctcagagattattcctaagtcatctatcaactacagtcattcttactgattgtctgcatgggttcctactttctgctagcattgtgttcatttttctcggagccacttctctgagggattacttcaggcatttacgtgagcttggaggtcaagatgttgacagagaagatgaagcagaaagaaatggcgctcgtcctgtaagaagagttcctggacaagctaataggaactttgttggggatgctaacggggaagatgctgctggagcacaagggattgctggagcaggtcaaatgattagaaggaatgcagaaaatgtggctgctcgctgggagatgcaggcagctcgtcttgaggctcatgttgaacagatgtttgatggtctagacgatgccgatggtgcagaggatgtaccatttgacgagcttgtgggcatgcagggacctgtatttcatttagtggaaaatgcatttactgtactggcaagcaatatgatattccttggtgtagtaatatttgtgcccttctcgttaggtcggattatactctatcatttatcttggcttttctccaccgctagtggtccagttttgtcaacagtcatgccactcactgaatcagccctttccttggctaatatgactttgaagaatgcattaacagctgtaacaaatttatcaaccgatagcgagaaaagtggtatggttggccaggttgcagagatcttgaaagttaacatgagtggactgaatgaggtttcaaacaataatgcaagttcaacactttcagcagacttcctgaaaggggcaacaattggaacttcaaggctctctgatgtgacaactcttgctattggatacatgtttatattctccctagttttcttctaccttggtattgtggctttcattcggtatactaggggtgagccactgactatggggaggttttatggtattgcttcaatggcagaaacaattccatctctcttcaggcagttcctggcagcaatgaggcatttgatgacaatgattaaggtcgctttccttctggtcattgaacttggagtgttcccattaatgtgtggatggtggttggatgtttgtactataaggatgtttgggaagtccatgtctcacagggtgcagttcttctcagcatctcccttagctagctccctggtccattgggttgtaggaatcgtatacatgctacaaataagcatattcgtcagtcttcttcgaggggttctgcgtaatggagttctttatttccttagagatccagctgatccaaactacaatcccttccgtgatctcattgatgatcctgtgcacaagcatgctcgcagggtcctgttatccgttgcggtgtatgggagtttaattgtgatgctggtgtttttaccagttaaactagctatgcggatggcaccttccatttttcctcttgacatttcggtatctgacccatttactgaaattcctgcggacatgcttctatttcaaatatgcattccatttgccattgagcatttcaaattacgggcaaccattaaatccctgctccgttattggtttacggcagttggctgggcacttggtttaacagatttcttactacccatacctgaggataacgttggacaggaaaatggaaatgcagagcctggaagacaattggggctacaggatcaggctttagtggcacttccaggtgctgatgaaccaaatcgaggccttcttgcgtcaggggacgcgaatgttgcagaggagtatgacactgatgaacaatctgattctgaaaggtatagctttgtgcttcgaattgtcctcttgttggtggtggcttggatgactttactcgtcttcaactcggccttgatagttgtaccaacatcactgggaagaacaattttcaatgtcattcctttcctccctataactcatggcatcaagtgcaacgatttatatgctttcatcattggaagctacataatatggactgctgtagctggagttagatattcaattgaacacattagaacaaaaagggttgcagttcttttgggccagatctggaagtggtgtagcattgtcattaagagttctgcactgttgtcaatatggatttttgtcattccagtattgatcgggttgctctttgagcttttggtgattgtgccgatgcgggtgcctgttgatgaaagcccagtattcctgctgtatcaggactgggctttaggccttatttttctcaagatatggacaagattggtgatgttggatcacatgatgccattggtagatgaaagctggcgcgtaaagtttgaaagggtgagggaagatggcttctccaggctgcaaggtctttgggtgctccgggagattgtattcccaatcataatgaagttgttgactgccctgtgtgtgccttatgtacttgcaagagggttatttcctgtgctcggttacccattagtagtcaactcagcagtgtatcggtttgcctggataggatgcctgtgctttagtctgctgtgcttctgtgccaagaggttccatgtatggttcaccaaccttcacaactctatacgtgatgaccgatatctaattggtcgtaggcttcataactttggagaagatgttgtagagaagcaaaatgaggcagggacatcctctgaattgcaggactctagttttgaagccagtggtttaattcctcatgaccgagaggctgacgatggtctccgtcaaaggcgtgctatccaacataatgcttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000209 GO:0001101 GO:0002020 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006508 GO:0006511 GO:0006629 GO:0006720 GO:0006725 GO:0006807 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009414 GO:0009415 GO:0009628 GO:0009698 GO:0009699 GO:0009893 GO:0009987 GO:0010025 GO:0010033 GO:0010035 GO:0010143 GO:0010166 GO:0010243 GO:0010345 GO:0010498 GO:0012505 GO:0016020 GO:0016021 GO:0016567 GO:0016740 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019787 GO:0019899 GO:0019941 GO:0030163 GO:0030176 GO:0030433 GO:0031224 GO:0031227 GO:0031624 GO:0031984 GO:0032446 GO:0032501 GO:0032502 GO:0033554 GO:0034976 GO:0036211 GO:0036503 GO:0042175 GO:0042221 GO:0042335 GO:0043085 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044390 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0048518 GO:0048856 GO:0050789 GO:0050790 GO:0050896 GO:0051341 GO:0051353 GO:0051603 GO:0051716 GO:0065007 GO:0065009 GO:0070647 GO:0070936 GO:0071704 GO:0098827 GO:0140096 GO:1900490 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901568 GO:1901570 GO:1901575 GO:1901576 GO:1901698 GO:1901700 GO:1990381 GO:2001215
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
RINGv

Protein Analysis

1016

Amino Acids

113.89

Weight (kDa)

6.31

Isoelectric Point (pI)

36.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 109
Acc36I ACCTGC 2 cut(s) 109, 545
AccB1I GGYRCC 2 cut(s) 1699, 2461
AccI GTMKAC 2 cut(s) 1184, 1473
AccII CGCG 2 cut(s) 2027, 2589
AciI CCGC 6 cut(s) 238, 843, 1637, 1693, 1757, 2458
AclI AACGTT 1 cut(s) 1908
AclWI GGATC 5 cut(s) 1544, 1553, 1589, 1968, 2562
AcoI YGGCCR 1 cut(s) 976
AcsI RAATTY 2 cut(s) 940, 1749
AcuI CTGAAG 2 cut(s) 376, 1243
AdeI CACNNNGTG 1 cut(s) 1411
AfaI GTAC 5 cut(s) 681, 747, 1375, 2160, 2709
AfiI CCNNNNNNNGG 5 cut(s) 898, 1072, 2003, 2107, 2735
AflIII ACRYGT 1 cut(s) 1128
AjnI CCWGG 8 cut(s) 478, 978, 1269, 1446, 1936, 1981, 2624, 2777
Alw21I GWGCWC 4 cut(s) 538, 1605, 2649, 2736
Alw26I GTCTC 2 cut(s) 1407, 3020
Alw44I GTGCAC 1 cut(s) 1601
AlwI GGATC 5 cut(s) 1544, 1553, 1589, 1968, 2562
AlwNI CAGNNNCTG 3 cut(s) 1270, 1937, 1988
AoxI GGCC 5 cut(s) 976, 2005, 2145, 2338, 2512
ApaLI GTGCAC 1 cut(s) 1601
ApeKI GCWGC 5 cut(s) 527, 590, 611, 1274, 2629
ApoI RAATTY 2 cut(s) 940, 1749
ArsI GACNNNNNNTTYG 2 cut(s) 2951, 2983
AseI ATTAAT 1 cut(s) 1346
Asp700I GAANNNNTTC 1 cut(s) 1245
AspLEI GCGC 3 cut(s) 177, 458, 2589
AspS9I GGNCC 5 cut(s) 710, 851, 1450, 1621, 2338
AsuC2I CCSGG 1 cut(s) 2651
AsuHPI GGTGA 6 cut(s) 275, 1205, 2455, 2557, 2617, 2833
AsuII TTCGAA 1 cut(s) 2092
AsuNHI GCTAGC 2 cut(s) 344, 1439
AvaII GGWCC 4 cut(s) 710, 851, 1450, 1621
AxyI CCTNAGG 1 cut(s) 1899
BaeGI GKGCMC 3 cut(s) 792, 1605, 1867
BalI TGGCCA 1 cut(s) 978
BanI GGYRCC 2 cut(s) 1699, 2461
BbsI GAAGAC 3 cut(s) 1496, 1948, 2128
Bbv12I GWGCWC 4 cut(s) 538, 1605, 2649, 2736
BbvI GCAGC 5 cut(s) 514, 577, 623, 1286, 2616
BccI CCATC 8 cut(s) 130, 641, 659, 1258, 1350, 1690, 2609, 3005
BceAI ACGGC 1 cut(s) 1866
BcgI CGANNNNNNTGC 6 cut(s) 596, 630, 2222, 2256, 2443, 2477
BciT130I CCWGG 8 cut(s) 480, 980, 1271, 1448, 1938, 1983, 2626, 2779
BciVI GTATCC 1 cut(s) 1118
BcnI CCSGG 1 cut(s) 2651
BcoDI GTCTC 2 cut(s) 1407, 3020
BfaI CTAG 9 cut(s) 105, 345, 653, 846, 1145, 1188, 1440, 1685, 2964
BfmI CTRYAG 3 cut(s) 300, 1955, 2277
BfoI RGCGCY 1 cut(s) 459
BfuAI ACCTGC 2 cut(s) 109, 545
BfuI GTATCC 1 cut(s) 1118
BglII AGATCT 2 cut(s) 990, 2343
BisI GCNGC 6 cut(s) 239, 528, 591, 612, 1275, 2630
BlsI GCNGC 6 cut(s) 240, 529, 592, 613, 1276, 2631
Bme1390I CCNGG 9 cut(s) 480, 980, 1271, 1448, 1938, 1983, 2626, 2651, 2779
Bme18I GGWCC 4 cut(s) 710, 851, 1450, 1621
BmgT120I GGNCC 5 cut(s) 710, 851, 1450, 1621, 2338
BmiI GGNNCC 8 cut(s) 203, 332, 370, 711, 1622, 1701, 2463, 2828
BmrFI CCNGG 9 cut(s) 480, 980, 1271, 1448, 1938, 1983, 2626, 2651, 2779
BmrI ACTGGG 3 cut(s) 2180, 2474, 2512
BmtI GCTAGC 2 cut(s) 348, 1443
BmuI ACTGGG 3 cut(s) 2180, 2474, 2512
BoxI GACNNNNGTC 1 cut(s) 3012
BpiI GAAGAC 3 cut(s) 1496, 1948, 2128
BpmI CTGGAG 4 cut(s) 552, 570, 2304, 2608
Bpu10I CCTNAGC 1 cut(s) 1435
Bpu14I TTCGAA 1 cut(s) 2092
BpuEI CTTGAG 2 cut(s) 641, 2510
BpuMI CCSGG 1 cut(s) 2651
BsaAI YACGTR 2 cut(s) 404, 2864
BsaI GGTCTC 1 cut(s) 3020
BsaJI CCNNGG 4 cut(s) 769, 897, 1159, 1446
BsaXI ACNNNNNCTCC 4 cut(s) 247, 277, 2898, 2928
Bsc4I CCNNNNNNNGG 5 cut(s) 898, 1072, 2003, 2107, 2735
Bse1I ACTGG 8 cut(s) 753, 854, 1676, 2175, 2411, 2480, 2507, 2976
Bse21I CCTNAGG 1 cut(s) 1899
Bse3DI GCAATG 2 cut(s) 147, 1284
BseBI CCWGG 8 cut(s) 480, 980, 1271, 1448, 1938, 1983, 2626, 2779
BseDI CCNNGG 4 cut(s) 769, 897, 1159, 1446
BseLI CCNNNNNNNGG 5 cut(s) 898, 1072, 2003, 2107, 2735
BseMI GCAATG 2 cut(s) 147, 1284
BseMII CTCAG 6 cut(s) 81, 284, 371, 1437, 1890, 2772
BseNI ACTGG 8 cut(s) 753, 854, 1676, 2175, 2411, 2480, 2507, 2976
BseRI GAGGAG 3 cut(s) 194, 268, 2054
BseSI GKGCMC 3 cut(s) 792, 1605, 1867
BseXI GCAGC 5 cut(s) 514, 577, 623, 1286, 2616
BseYI CCCAGC 2 cut(s) 597, 1860
BsgI GTGCAG 3 cut(s) 690, 1433, 2364
Bsh1236I CGCG 2 cut(s) 2027, 2589
BshFI GGCC 5 cut(s) 978, 2007, 2147, 2340, 2514
BshNI GGYRCC 2 cut(s) 1699, 2461
BsiHKAI GWGCWC 4 cut(s) 538, 1605, 2649, 2736
BsiSI CCGG 1 cut(s) 2650
BslFI GGGAC 3 cut(s) 723, 2036, 2953
BslI CCNNNNNNNGG 5 cut(s) 898, 1072, 2003, 2107, 2735
BsmAI GTCTC 2 cut(s) 1407, 3020
BsmFI GGGAC 3 cut(s) 723, 2036, 2953
BsmI GAATGC 4 cut(s) 100, 580, 925, 1782
BsnI GGCC 5 cut(s) 978, 2007, 2147, 2340, 2514
Bso31I GGTCTC 1 cut(s) 3020
Bsp119I TTCGAA 1 cut(s) 2092
Bsp1286I GDGCHC 6 cut(s) 538, 792, 1605, 1867, 2649, 2736
Bsp143I GATC 9 cut(s) 990, 1549, 1558, 1582, 1594, 1960, 2343, 2418, 2554
BspACI CCGC 6 cut(s) 238, 843, 1637, 1693, 1757, 2458
BspANI GGCC 5 cut(s) 978, 2007, 2147, 2340, 2514
BspCNI CTCAG 6 cut(s) 82, 283, 372, 1436, 1891, 2771
BspFNI CGCG 2 cut(s) 2027, 2589
BspHI TCATGA 1 cut(s) 2992
BspLI GGNNCC 8 cut(s) 203, 332, 370, 711, 1622, 1701, 2463, 2828
BspMI ACCTGC 2 cut(s) 109, 545
BspOI GCTAGC 2 cut(s) 348, 1443
BspPI GGATC 5 cut(s) 1544, 1553, 1589, 1968, 2562
BspT104I TTCGAA 1 cut(s) 2092
BspT107I GGYRCC 2 cut(s) 1699, 2461
BspTNI GGTCTC 1 cut(s) 3020
BsrDI GCAATG 2 cut(s) 147, 1284
BsrI ACTGG 8 cut(s) 753, 854, 1676, 2175, 2411, 2480, 2507, 2976
BssECI CCNNGG 4 cut(s) 769, 897, 1159, 1446
BssMI GATC 9 cut(s) 990, 1549, 1558, 1582, 1594, 1960, 2343, 2418, 2554
BssNAI GTATAC 2 cut(s) 1185, 1474
BssT1I CCWWGG 3 cut(s) 769, 897, 1159
Bst1107I GTATAC 2 cut(s) 1185, 1474
Bst2UI CCWGG 8 cut(s) 480, 980, 1271, 1448, 1938, 1983, 2626, 2779
Bst4CI ACNGT 4 cut(s) 304, 745, 868, 2385
Bst6I CTCTTC 2 cut(s) 466, 1262
BstAPI GCANNNNNTGC 1 cut(s) 2226
BstBAI YACGTR 2 cut(s) 404, 2864
BstBI TTCGAA 1 cut(s) 2092
BstEII GGTNACC 1 cut(s) 2738
BstFNI CGCG 2 cut(s) 2027, 2589
BstH2I RGCGCY 1 cut(s) 459
BstHHI GCGC 3 cut(s) 177, 458, 2589
BstKTI GATC 9 cut(s) 993, 1552, 1561, 1585, 1597, 1963, 2346, 2421, 2557
BstMAI GTCTC 2 cut(s) 1407, 3020
BstMBI GATC 9 cut(s) 990, 1549, 1558, 1582, 1594, 1960, 2343, 2418, 2554
BstMWI GCNNNNNNNGC 9 cut(s) 95, 533, 668, 929, 1235, 1973, 2033, 2226, 2434
BstNI CCWGG 8 cut(s) 480, 980, 1271, 1448, 1938, 1983, 2626, 2779
BstNSI RCATGY 5 cut(s) 706, 1132, 1480, 1613, 1765
BstPAI GACNNNNGTC 1 cut(s) 3012
BstPI GGTNACC 1 cut(s) 2738
BstSCI CCNGG 9 cut(s) 478, 978, 1269, 1446, 1936, 1981, 2624, 2649, 2777
BstSFI CTRYAG 3 cut(s) 300, 1955, 2277
BstSLI GKGCMC 3 cut(s) 792, 1605, 1867
BstUI CGCG 2 cut(s) 2027, 2589
BstV1I GCAGC 5 cut(s) 514, 577, 623, 1286, 2616
BstV2I GAAGAC 3 cut(s) 1496, 1948, 2128
BstX2I RGATCY 3 cut(s) 990, 1549, 2343
BstXI CCANNNNNNTGG 1 cut(s) 49
BstYI RGATCY 3 cut(s) 990, 1549, 2343
BstZ17I GTATAC 2 cut(s) 1185, 1474
Bsu36I CCTNAGG 1 cut(s) 1899
BsuI GTATCC 1 cut(s) 1118
BsuRI GGCC 5 cut(s) 978, 2007, 2147, 2340, 2514
BtsI GCAGTG 1 cut(s) 2769
BtsIMutI CAGTG 7 cut(s) 879, 1199, 2049, 2168, 2381, 2769, 2983
BveI ACCTGC 2 cut(s) 109, 545
CaiI CAGNNNCTG 3 cut(s) 1270, 1937, 1988
CciI TCATGA 1 cut(s) 2992
CfoI GCGC 3 cut(s) 177, 458, 2589
Cfr13I GGNCC 5 cut(s) 710, 851, 1450, 1621, 2338
CseI GACGC 2 cut(s) 2004, 2033
Csp6I GTAC 5 cut(s) 680, 746, 1374, 2159, 2708
CviQI GTAC 5 cut(s) 680, 746, 1374, 2159, 2708
DpnI GATC 9 cut(s) 992, 1551, 1560, 1584, 1596, 1962, 2345, 2420, 2556
DpnII GATC 9 cut(s) 990, 1549, 1558, 1582, 1594, 1960, 2343, 2418, 2554
DraIII CACNNNGTG 1 cut(s) 1411
EaeI YGGCCR 1 cut(s) 976
Eam1104I CTCTTC 2 cut(s) 466, 1262
EarI CTCTTC 2 cut(s) 466, 1262
Eco130I CCWWGG 3 cut(s) 769, 897, 1159
Eco147I AGGCCT 2 cut(s) 2007, 2514
Eco31I GGTCTC 1 cut(s) 3020
Eco32I GATATC 1 cut(s) 2876
Eco47I GGWCC 4 cut(s) 710, 851, 1450, 1621
Eco57I CTGAAG 2 cut(s) 376, 1243
Eco81I CCTNAGG 1 cut(s) 1899
Eco91I GGTNACC 1 cut(s) 2738
EcoO109I RGGNCCY 2 cut(s) 710, 1621
EcoO65I GGTNACC 1 cut(s) 2738
EcoRII CCWGG 8 cut(s) 478, 978, 1269, 1446, 1936, 1981, 2624, 2777
EcoRV GATATC 1 cut(s) 2876
EcoT14I CCWWGG 3 cut(s) 769, 897, 1159
EcoT22I ATGCAT 3 cut(s) 739, 925, 1784
ErhI CCWWGG 3 cut(s) 769, 897, 1159
FaqI GGGAC 3 cut(s) 723, 2036, 2953
FauI CCCGC 1 cut(s) 2451
FblI GTMKAC 2 cut(s) 1184, 1473
Fnu4HI GCNGC 6 cut(s) 239, 528, 591, 612, 1275, 2630
Fsp4HI GCNGC 6 cut(s) 239, 528, 591, 612, 1275, 2630
FspBI CTAG 9 cut(s) 105, 345, 653, 846, 1145, 1188, 1440, 1685, 2964
GlaI GCGC 3 cut(s) 176, 457, 2588
GluI GCNGC 6 cut(s) 239, 528, 591, 612, 1275, 2630
GsaI CCCAGC 2 cut(s) 601, 1864
GsuI CTGGAG 4 cut(s) 552, 570, 2304, 2608
HaeII RGCGCY 1 cut(s) 459
HaeIII GGCC 5 cut(s) 978, 2007, 2147, 2340, 2514
HapII CCGG 1 cut(s) 2650
HgaI GACGC 2 cut(s) 2004, 2033
HhaI GCGC 3 cut(s) 177, 458, 2589
Hin6I GCGC 3 cut(s) 175, 456, 2587
HinP1I GCGC 3 cut(s) 175, 456, 2587
HincII GTYRAC 5 cut(s) 427, 864, 1003, 2685, 2755
HindII GTYRAC 5 cut(s) 427, 864, 1003, 2685, 2755
HinfI GANTC 5 cut(s) 31, 884, 1467, 2066, 2960
HpaI GTTAAC 1 cut(s) 1003
HpaII CCGG 1 cut(s) 2650
HphI GGTGA 6 cut(s) 275, 1205, 2455, 2557, 2617, 2833
Hpy188I TCNGA 9 cut(s) 273, 368, 381, 807, 1102, 1735, 2065, 2071, 2950
HpyAV CCTTC 8 cut(s) 82, 564, 802, 1325, 1585, 1713, 2018, 2858
HpyCH4III ACNGT 4 cut(s) 304, 745, 868, 2385
HpyCH4IV ACGT 3 cut(s) 403, 1908, 2863
HpyF10VI GCNNNNNNNGC 9 cut(s) 95, 533, 668, 929, 1235, 1973, 2033, 2226, 2434
HpySE526I ACGT 3 cut(s) 403, 1908, 2863
HspAI GCGC 3 cut(s) 175, 456, 2587
KspAI GTTAAC 1 cut(s) 1003
Kzo9I GATC 9 cut(s) 990, 1549, 1558, 1582, 1594, 1960, 2343, 2418, 2554
LmnI GCTCC 7 cut(s) 207, 368, 533, 551, 1448, 1839, 2652
Lsp1109I GCAGC 5 cut(s) 514, 577, 623, 1286, 2616
MaeI CTAG 9 cut(s) 105, 345, 653, 846, 1145, 1188, 1440, 1685, 2964
MaeII ACGT 3 cut(s) 403, 1908, 2863
MaeIII GTNAC 3 cut(s) 934, 1105, 2738
MalI GATC 9 cut(s) 992, 1551, 1560, 1584, 1596, 1962, 2345, 2420, 2556
MboI GATC 9 cut(s) 990, 1549, 1558, 1582, 1594, 1960, 2343, 2418, 2554
MfeI CAATTG 3 cut(s) 1080, 1946, 2298
MflI RGATCY 3 cut(s) 990, 1549, 2343
MhlI GDGCHC 6 cut(s) 538, 792, 1605, 1867, 2649, 2736
MlsI TGGCCA 1 cut(s) 978
MluNI TGGCCA 1 cut(s) 978
MlyI GAGTC 1 cut(s) 2954
MmeI TCCRAC 4 cut(s) 785, 1344, 1891, 2532
Mox20I TGGCCA 1 cut(s) 978
Mph1103I ATGCAT 3 cut(s) 739, 925, 1784
MroXI GAANNNNTTC 1 cut(s) 1245
MscI TGGCCA 1 cut(s) 978
MslI CAYNNNNRTG 3 cut(s) 402, 1348, 1608
Msp20I TGGCCA 1 cut(s) 978
MspA1I CMGCKG 2 cut(s) 932, 1556
MspI CCGG 1 cut(s) 2650
MspR9I CCNGG 9 cut(s) 480, 980, 1271, 1448, 1938, 1983, 2626, 2651, 2779
MunI CAATTG 3 cut(s) 1080, 1946, 2298
Mva1269I GAATGC 4 cut(s) 100, 580, 925, 1782
MvaI CCWGG 8 cut(s) 480, 980, 1271, 1448, 1938, 1983, 2626, 2779
MvnI CGCG 2 cut(s) 2027, 2589
MwoI GCNNNNNNNGC 9 cut(s) 95, 533, 668, 929, 1235, 1973, 2033, 2226, 2434
NciI CCSGG 1 cut(s) 2651
NdeII GATC 9 cut(s) 990, 1549, 1558, 1582, 1594, 1960, 2343, 2418, 2554
NheI GCTAGC 2 cut(s) 344, 1439
NlaIV GGNNCC 8 cut(s) 203, 332, 370, 711, 1622, 1701, 2463, 2828
NmeAIII GCCGAG 1 cut(s) 2123
NmuCI GTSAC 1 cut(s) 1105
NsiI ATGCAT 3 cut(s) 739, 925, 1784
NspI RCATGY 5 cut(s) 706, 1132, 1480, 1613, 1765
NspV TTCGAA 1 cut(s) 2092
PaeI GCATGC 2 cut(s) 706, 1613
PagI TCATGA 1 cut(s) 2992
PaqCI CACCTGC 1 cut(s) 109
PceI AGGCCT 2 cut(s) 2007, 2514
PciI ACATGT 1 cut(s) 1128
PcsI WCGNNNNNNNCGW 1 cut(s) 2869
PctI GAATGC 4 cut(s) 100, 580, 925, 1782
PdmI GAANNNNTTC 1 cut(s) 1245
PfeI GAWTC 4 cut(s) 31, 884, 1467, 2066
PfoI TCCNGGA 2 cut(s) 478, 2649
PkrI GCNGC 6 cut(s) 240, 529, 592, 613, 1276, 2631
PleI GAGTC 1 cut(s) 2954
PpsI GAGTC 1 cut(s) 2954
Ppu21I YACGTR 2 cut(s) 404, 2864
PpuMI RGGWCCY 2 cut(s) 710, 1621
PscI ACATGT 1 cut(s) 1128
PshAI GACNNNNGTC 1 cut(s) 3012
PshBI ATTAAT 1 cut(s) 1346
Psp1406I AACGTT 1 cut(s) 1908
Psp5II RGGWCCY 2 cut(s) 710, 1621
Psp6I CCWGG 8 cut(s) 478, 978, 1269, 1446, 1936, 1981, 2624, 2777
PspEI GGTNACC 1 cut(s) 2738
PspFI CCCAGC 2 cut(s) 597, 1860
PspGI CCWGG 8 cut(s) 478, 978, 1269, 1446, 1936, 1981, 2624, 2777
PspN4I GGNNCC 8 cut(s) 203, 332, 370, 711, 1622, 1701, 2463, 2828
PspPI GGNCC 5 cut(s) 710, 851, 1450, 1621, 2338
PspPPI RGGWCCY 2 cut(s) 710, 1621
PsrI GAACNNNNNNTAC 2 cut(s) 459, 491
PstNI CAGNNNCTG 3 cut(s) 1270, 1937, 1988
PsuI RGATCY 3 cut(s) 990, 1549, 2343
PvuII CAGCTG 2 cut(s) 932, 1556
RsaI GTAC 5 cut(s) 681, 747, 1375, 2160, 2709
RsaNI GTAC 5 cut(s) 680, 746, 1374, 2159, 2708
RseI CAYNNNNRTG 3 cut(s) 402, 1348, 1608
SatI GCNGC 6 cut(s) 239, 528, 591, 612, 1275, 2630
Sau3AI GATC 9 cut(s) 990, 1549, 1558, 1582, 1594, 1960, 2343, 2418, 2554
Sau96I GGNCC 5 cut(s) 710, 851, 1450, 1621, 2338
SchI GAGTC 1 cut(s) 2954
ScrFI CCNGG 9 cut(s) 480, 980, 1271, 1448, 1938, 1983, 2626, 2651, 2779
SduI GDGCHC 6 cut(s) 538, 792, 1605, 1867, 2649, 2736
SfcI CTRYAG 3 cut(s) 300, 1955, 2277
SfuI TTCGAA 1 cut(s) 2092
SinI GGWCC 4 cut(s) 710, 851, 1450, 1621
SmiMI CAYNNNNRTG 3 cut(s) 402, 1348, 1608
SmlI CTYRAG 2 cut(s) 620, 2525
SmoI CTYRAG 2 cut(s) 620, 2525
SphI GCATGC 2 cut(s) 706, 1613
SseBI AGGCCT 2 cut(s) 2007, 2514
SsiI CCGC 6 cut(s) 238, 843, 1637, 1693, 1757, 2458
SspI AATATT 1 cut(s) 783
SspMI CTAG 9 cut(s) 105, 345, 653, 846, 1145, 1188, 1440, 1685, 2964
StuI AGGCCT 2 cut(s) 2007, 2514
StyD4I CCNGG 9 cut(s) 478, 978, 1269, 1446, 1936, 1981, 2624, 2649, 2777
StyI CCWWGG 3 cut(s) 769, 897, 1159
TaaI ACNGT 4 cut(s) 304, 745, 868, 2385
TaiI ACGT 3 cut(s) 406, 1911, 2866
TaqI TCGA 4 cut(s) 29, 1510, 2002, 2092
TaqII GACCGA 2 cut(s) 2886, 3012
TatI WGTACW 3 cut(s) 745, 1373, 2707
TauI GCSGC 1 cut(s) 241
TfiI GAWTC 4 cut(s) 31, 884, 1467, 2066
TscAI CASTG 7 cut(s) 886, 1206, 2056, 2175, 2388, 2769, 2983
TseFI GTSAC 1 cut(s) 1105
TseI GCWGC 5 cut(s) 527, 590, 611, 1274, 2629
Tsp45I GTSAC 1 cut(s) 1105
TspGWI ACGGA 6 cut(s) 24, 184, 1568, 1621, 1826, 3008
TspRI CASTG 7 cut(s) 886, 1206, 2056, 2175, 2388, 2769, 2983
VneI GTGCAC 1 cut(s) 1601
VpaK11BI GGWCC 4 cut(s) 710, 851, 1450, 1621
VspI ATTAAT 1 cut(s) 1346
XapI RAATTY 2 cut(s) 940, 1749
XbaI TCTAGA 1 cut(s) 652
XceI RCATGY 5 cut(s) 706, 1132, 1480, 1613, 1765
XmiI GTMKAC 2 cut(s) 1184, 1473
XmnI GAANNNNTTC 1 cut(s) 1245
XspI CTAG 9 cut(s) 105, 345, 653, 846, 1145, 1188, 1440, 1685, 2964
Zsp2I ATGCAT 3 cut(s) 739, 925, 1784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.