Rh6DG069300

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0009109
Physical Location & Seq
Reverse (-)
7295064 .. 7301544
6481 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG069300.1

Sequence Viewer

Length: 2352 bp
ATGGAGATCGCCCCGGCGCCGCCACAGCTTTCAGACCGGGACGTCCCAAACGACGCCGCGTCGCTAAGCACCGTTAAAACGGCGCCGTCGGAGAGCAACAACGAGGCGAACGTGTCGACGTCGTCGTCGTCGGTGGCGGGGAAGTACGACGATGAGGAAGAGGAGGAGGACGTGTGCCGGATCTGTAGGAACCCTGGCGACGCCGATAACCCGCTTAGGTATCCATGCGCGTGTAGCGGAAGCATCAAGTTCGTGCACCAGGATTGTCTCCTTCAGTGGCTCAATCACAGTAATGCTCGCCAATGCGAGGTTTGCAAGCATCCATTTTCCTTCTCTCCCGTTTATGCTGAGAATGCACCTGCTAGGCTTCCTTTTCAAGAGTTTGTGGTTGGGATGGCAATGAAAACTTGTCATGTTCTGCAATTTTTCCTGCGCCTTAGTTTTGTGCTTTCCGTTTGGCTCCTCATTATTCCTTTCATCACATTTTGGATATGGCGGCTGGCTTTCGTGAGGAGTTTTGGTGAAGCTCAGAGATTATTCCTAAGTCATCTATCAACTACAGTCATTCTTACTGATTGTCTGCATGGGTTCCTACTTTCTGCTAGCATTGTGTTCATTTTTCTCGGAGCCACTTCTCTGAGGGATTACTTCAGGCATTTACGTGAGCTTGGAGGTCAAGATGTTGACAGAGAAGATGAAGCAGAAAGAAATGGCGCTCGTCCTGTAAGAAGAGTTCCTGGACAAGCTAATAGGAACTTTGTTGGGGATGCTAACGGGGAAGATGCTGCTGGAGCACAAGGGATTGCTGGAGCAGGTCAAATGATTAGAAGGAATGCTGAAAATGTGGCTGCTCGCTGGGAGATGCAGGCAGCTCGTCTTGAGGCTCATGTTGAACAGATGTTTGATGGTCTAGACGATGCCGATGGTGCAGAGGATGTACCATTTGACGAGCTTGTGGGCATGCAGGGACCTGTATTTCATTTAGTGGAAAATGCATTTACTGTACTGGCAAGCAATATGATATTCCTTGGTGTAGTAATATTTGTGCCCTTCTCGTTAGGTCGGATTATACTCTATCATTTATCTTGGCTTTTCTCCACCGCTAGTGGTCCAGTTTTGTCAACAGTCATGCCACTCACTGAATCAGCCCTTTCCTTGGCTAATATGACTTTGAAGAATGCATTAACAGCTGTAACAAATTTATCAACCGATAGCGAGAAAAGTGGTATGGTTGGCCAGGTTGCAGAGATCTTGAAAGTTAACATGAGTGGACTGAATGAGGTTTCGAACAATAATGCAAGTTCAACACTTTCAGCAGACTTCCTGAAAGGGGCAACAATTGGAACTTCAAGGCTCTCTGATGTGACAACTCTTGCTATTGGATACATGTTTATATTCTCCCTAGTTTTCTTCTACCTTGGTATTGTGGCTTTCATTCGGTATACTAGGGGTGAGCCATTGACTATGGGGAGGTTTTATGGTATTGCTTCAATGGCAGAAACAATTCCATCTCTCTTCAGGCAGTTCCTGGCAGCAATGAGGCATTTGATGACAATGATTAAGGTCGCTTTCCTTCTGGTCATTGAACTTGGAGTGTTCCCATTAATGTGTGGATGGTGGTTGGATGTTTGTACTATAAGGATGTTTGGGAAGTCCATGTCTCACAGGGTGCAGTTCTTTTCAGCATCTCCCTTAGCTAGCTCCCTGGTCCATTGGGTTGTAGGAATCGTATACATGCTACAAATAAGCATATTCGTCAGTCTTCTTCGAGGGGTTCTGCGTAATGGAGTTCTTTATTTCCTTAGAGATCCAGCTGATCCAAACTACAATCCCTTCCGTGATCTCATTGATGATCCTGTGCACAAGCATGCTCGCAGGGTACTGTTATCCGTTGCGGTGTATGGGAGTTTAATTGTGATGCTGGTGTTTTTACCAGTTAAACTAGCTATGCGGATGGCACCTTCCATTTTTCCTCTTGACATTTCGGTATCTGACCCATTTACTGAAATTCCTGCGGACATGCTTCTATTTCAAATATGCATTCCATTTGCCATTGAGCATTTCAAATTACGGGCAACCATTAAATCCCTGCTCCGTTATTGGTTTACGGCAGTTGGCTGGGCACTTGGTTTAACAGATTTCTTACTACCCAGACCTGAGGATAACGTTGGACAGGAAAATGGAAATGCAGAGCCTGGAAGACAGGATAGACTACAGGTTCAATTGGGGCTACAGGATCAGGCTTTAGTGGCACTTCCAGGTGCTGATGAACCAAATCGAGGCCTTCTTGCATCAGGGGACGCGAATGTTGCAGAGGAGTATGACACTGATGAACAATCTGATTCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000209 GO:0001101 GO:0002020 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006508 GO:0006511 GO:0006629 GO:0006720 GO:0006725 GO:0006807 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009414 GO:0009415 GO:0009628 GO:0009698 GO:0009699 GO:0009893 GO:0009987 GO:0010025 GO:0010033 GO:0010035 GO:0010143 GO:0010166 GO:0010243 GO:0010345 GO:0010498 GO:0012505 GO:0016020 GO:0016021 GO:0016567 GO:0016740 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019787 GO:0019899 GO:0019941 GO:0030163 GO:0030176 GO:0030433 GO:0031224 GO:0031227 GO:0031624 GO:0031984 GO:0032446 GO:0032501 GO:0032502 GO:0033554 GO:0034976 GO:0036211 GO:0036503 GO:0042175 GO:0042221 GO:0042335 GO:0043085 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044390 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0048518 GO:0048856 GO:0050789 GO:0050790 GO:0050896 GO:0051341 GO:0051353 GO:0051603 GO:0051716 GO:0065007 GO:0065009 GO:0070647 GO:0070936 GO:0071704 GO:0098827 GO:0140096 GO:1900490 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901568 GO:1901570 GO:1901575 GO:1901576 GO:1901698 GO:1901700 GO:1990381 GO:2001215
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
RINGv

Protein Analysis

783

Amino Acids

86.61

Weight (kDa)

5.16

Isoelectric Point (pI)

41.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 59 - 105 6.5e-17 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 367
AasI GACNNNNNNGTC 2 cut(s) 41, 124
AatII GACGTC 2 cut(s) 45, 122
Acc36I ACCTGC 2 cut(s) 367, 803
AccB1I GGYRCC 3 cut(s) 16, 82, 1957
AccI GTMKAC 3 cut(s) 116, 1442, 1731
AccII CGCG 3 cut(s) 59, 230, 2303
AclI AACGTT 1 cut(s) 2166
AclWI GGATC 5 cut(s) 188, 1802, 1811, 1847, 2244
AcoI YGGCCR 1 cut(s) 1234
AcsI RAATTY 2 cut(s) 1198, 2007
AcuI CTGAAG 3 cut(s) 257, 634, 1501
AcyI GRCGYC 6 cut(s) 17, 42, 54, 83, 119, 201
AdeI CACNNNGTG 1 cut(s) 1669
AfaI GTAC 5 cut(s) 146, 939, 1005, 1633, 1881
AfiI CCNNNNNNNGG 4 cut(s) 307, 1156, 1330, 2279
AflIII ACRYGT 3 cut(s) 111, 171, 1386
AhdI GACNNNNNGTC 1 cut(s) 58
AjiI CACGTC 1 cut(s) 172
AjnI CCWGG 8 cut(s) 193, 258, 736, 1236, 1527, 1704, 2194, 2257
Alw21I GWGCWC 3 cut(s) 258, 796, 1863
Alw26I GTCTC 2 cut(s) 272, 1665
Alw44I GTGCAC 2 cut(s) 254, 1859
AlwI GGATC 5 cut(s) 188, 1802, 1811, 1847, 2244
AlwNI CAGNNNCTG 3 cut(s) 1528, 2195, 2264
AoxI GGCC 2 cut(s) 1234, 2281
ApaLI GTGCAC 2 cut(s) 254, 1859
ApeKI GCWGC 4 cut(s) 785, 848, 869, 1532
ApoI RAATTY 2 cut(s) 1198, 2007
AseI ATTAAT 1 cut(s) 1604
Asp700I GAANNNNTTC 1 cut(s) 1503
AspLEI GCGC 5 cut(s) 19, 85, 230, 435, 716
AspS9I GGNCC 3 cut(s) 968, 1109, 1708
AsuC2I CCSGG 2 cut(s) 14, 38
AsuHPI GGTGA 2 cut(s) 533, 1463
AsuII TTCGAA 1 cut(s) 1286
AsuNHI GCTAGC 2 cut(s) 602, 1697
AvaII GGWCC 3 cut(s) 968, 1109, 1708
AxyI CCTNAGG 1 cut(s) 2157
BaeGI GKGCMC 4 cut(s) 258, 1050, 1863, 2125
BalI TGGCCA 1 cut(s) 1236
BanI GGYRCC 3 cut(s) 16, 82, 1957
BbsI GAAGAC 2 cut(s) 1754, 2206
Bbv12I GWGCWC 3 cut(s) 258, 796, 1863
BbvI GCAGC 4 cut(s) 772, 835, 881, 1544
BccI CCATC 6 cut(s) 388, 899, 917, 1516, 1608, 1948
BceAI ACGGC 3 cut(s) 70, 96, 2124
BcgI CGANNNNNNTGC 4 cut(s) 232, 266, 854, 888
BciT130I CCWGG 8 cut(s) 195, 260, 738, 1238, 1529, 1706, 2196, 2259
BciVI GTATCC 2 cut(s) 231, 1376
BcnI CCSGG 2 cut(s) 14, 38
BcoDI GTCTC 2 cut(s) 272, 1665
BfaI CTAG 8 cut(s) 363, 603, 911, 1104, 1403, 1446, 1698, 1943
BfmI CTRYAG 4 cut(s) 184, 558, 2213, 2231
BfoI RGCGCY 3 cut(s) 20, 86, 717
BfuAI ACCTGC 2 cut(s) 367, 803
BfuI GTATCC 2 cut(s) 231, 1376
BglII AGATCT 1 cut(s) 1248
BisI GCNGC 7 cut(s) 20, 57, 497, 786, 849, 870, 1533
BlpI GCTNAGC 1 cut(s) 65
BlsI GCNGC 7 cut(s) 21, 58, 498, 787, 850, 871, 1534
Bme18I GGWCC 3 cut(s) 968, 1109, 1708
BmeRI GACNNNNNGTC 1 cut(s) 58
BmgBI CACGTC 1 cut(s) 172
BmgT120I GGNCC 3 cut(s) 968, 1109, 1708
BmiI GGNNCC 8 cut(s) 18, 84, 191, 461, 590, 628, 969, 1959
BmsI GCATC 9 cut(s) 252, 328, 757, 772, 852, 907, 1694, 1908, 2300
BmtI GCTAGC 2 cut(s) 606, 1701
BpiI GAAGAC 2 cut(s) 1754, 2206
BpmI CTGGAG 2 cut(s) 810, 828
Bpu10I CCTNAGC 2 cut(s) 215, 1693
Bpu1102I GCTNAGC 1 cut(s) 65
Bpu14I TTCGAA 1 cut(s) 1286
BpuEI CTTGAG 1 cut(s) 899
BpuMI CCSGG 2 cut(s) 14, 38
BsaAI YACGTR 1 cut(s) 662
BsaHI GRCGYC 6 cut(s) 17, 42, 54, 83, 119, 201
BsaJI CCNNGG 6 cut(s) 12, 193, 1027, 1155, 1417, 1704
BsaXI ACNNNNNCTCC 4 cut(s) 158, 188, 505, 535
Bsc4I CCNNNNNNNGG 4 cut(s) 307, 1156, 1330, 2279
Bse1I ACTGG 3 cut(s) 1011, 1112, 1934
Bse21I CCTNAGG 1 cut(s) 2157
Bse3DI GCAATG 2 cut(s) 405, 1542
BseBI CCWGG 8 cut(s) 195, 260, 738, 1238, 1529, 1706, 2196, 2259
BseDI CCNNGG 6 cut(s) 12, 193, 1027, 1155, 1417, 1704
BseGI GGATG 8 cut(s) 319, 399, 772, 940, 1619, 1630, 1647, 1959
BseLI CCNNNNNNNGG 4 cut(s) 307, 1156, 1330, 2279
BseMI GCAATG 2 cut(s) 405, 1542
BseMII CTCAG 5 cut(s) 339, 542, 629, 2148, 2337
BseNI ACTGG 3 cut(s) 1011, 1112, 1934
BseRI GAGGAG 5 cut(s) 176, 179, 452, 526, 2330
BseSI GKGCMC 4 cut(s) 258, 1050, 1863, 2125
BseXI GCAGC 4 cut(s) 772, 835, 881, 1544
BseYI CCCAGC 2 cut(s) 855, 2118
BsgI GTGCAG 2 cut(s) 948, 1691
Bsh1236I CGCG 3 cut(s) 59, 230, 2303
BshFI GGCC 2 cut(s) 1236, 2283
BshNI GGYRCC 3 cut(s) 16, 82, 1957
BsiHKAI GWGCWC 3 cut(s) 258, 796, 1863
BsiSI CCGG 3 cut(s) 14, 37, 178
BslFI GGGAC 4 cut(s) 29, 53, 981, 2312
BslI CCNNNNNNNGG 4 cut(s) 307, 1156, 1330, 2279
BsmAI GTCTC 2 cut(s) 272, 1665
BsmFI GGGAC 4 cut(s) 29, 53, 981, 2312
BsmI GAATGC 4 cut(s) 358, 838, 1183, 2040
BsnI GGCC 2 cut(s) 1236, 2283
Bsp119I TTCGAA 1 cut(s) 1286
Bsp1286I GDGCHC 5 cut(s) 258, 796, 1050, 1863, 2125
Bsp143I GATC 8 cut(s) 6, 180, 1248, 1807, 1816, 1840, 1852, 2236
Bsp1720I GCTNAGC 1 cut(s) 65
BspANI GGCC 2 cut(s) 1236, 2283
BspCNI CTCAG 5 cut(s) 340, 541, 630, 2149, 2338
BspFNI CGCG 3 cut(s) 59, 230, 2303
BspLI GGNNCC 8 cut(s) 18, 84, 191, 461, 590, 628, 969, 1959
BspMI ACCTGC 2 cut(s) 367, 803
BspOI GCTAGC 2 cut(s) 606, 1701
BspPI GGATC 5 cut(s) 188, 1802, 1811, 1847, 2244
BspT104I TTCGAA 1 cut(s) 1286
BspT107I GGYRCC 3 cut(s) 16, 82, 1957
BsrDI GCAATG 2 cut(s) 405, 1542
BsrI ACTGG 3 cut(s) 1011, 1112, 1934
BssECI CCNNGG 6 cut(s) 12, 193, 1027, 1155, 1417, 1704
BssMI GATC 8 cut(s) 6, 180, 1248, 1807, 1816, 1840, 1852, 2236
BssNAI GTATAC 2 cut(s) 1443, 1732
BssNI GRCGYC 6 cut(s) 17, 42, 54, 83, 119, 201
BssT1I CCWWGG 3 cut(s) 1027, 1155, 1417
Bst1107I GTATAC 2 cut(s) 1443, 1732
Bst2UI CCWGG 8 cut(s) 195, 260, 738, 1238, 1529, 1706, 2196, 2259
Bst4CI ACNGT 6 cut(s) 73, 290, 562, 1003, 1126, 1884
Bst6I CTCTTC 3 cut(s) 153, 724, 1520
BstACI GRCGYC 6 cut(s) 17, 42, 54, 83, 119, 201
BstBAI YACGTR 1 cut(s) 662
BstBI TTCGAA 1 cut(s) 1286
BstF5I GGATG 8 cut(s) 319, 399, 772, 940, 1619, 1630, 1647, 1959
BstFNI CGCG 3 cut(s) 59, 230, 2303
BstH2I RGCGCY 3 cut(s) 20, 86, 717
BstHHI GCGC 5 cut(s) 19, 85, 230, 435, 716
BstKTI GATC 8 cut(s) 9, 183, 1251, 1810, 1819, 1843, 1855, 2239
BstMAI GTCTC 2 cut(s) 272, 1665
BstMBI GATC 8 cut(s) 6, 180, 1248, 1807, 1816, 1840, 1852, 2236
BstNI CCWGG 8 cut(s) 195, 260, 738, 1238, 1529, 1706, 2196, 2259
BstNSI RCATGY 5 cut(s) 964, 1390, 1738, 1871, 2023
BstSFI CTRYAG 4 cut(s) 184, 558, 2213, 2231
BstSLI GKGCMC 4 cut(s) 258, 1050, 1863, 2125
BstUI CGCG 3 cut(s) 59, 230, 2303
BstV1I GCAGC 4 cut(s) 772, 835, 881, 1544
BstV2I GAAGAC 2 cut(s) 1754, 2206
BstX2I RGATCY 3 cut(s) 180, 1248, 1807
BstYI RGATCY 3 cut(s) 180, 1248, 1807
BstZ17I GTATAC 2 cut(s) 1443, 1732
Bsu36I CCTNAGG 1 cut(s) 2157
BsuI GTATCC 2 cut(s) 231, 1376
BsuRI GGCC 2 cut(s) 1236, 2283
BtrI CACGTC 1 cut(s) 172
BtsCI GGATG 8 cut(s) 319, 399, 772, 940, 1619, 1630, 1647, 1959
BtsIMutI CAGTG 3 cut(s) 281, 1137, 2325
BveI ACCTGC 2 cut(s) 367, 803
CaiI CAGNNNCTG 3 cut(s) 1528, 2195, 2264
CfoI GCGC 5 cut(s) 19, 85, 230, 435, 716
Cfr13I GGNCC 3 cut(s) 968, 1109, 1708
CseI GACGC 4 cut(s) 48, 62, 209, 2309
Csp6I GTAC 5 cut(s) 145, 938, 1004, 1632, 1880
CviQI GTAC 5 cut(s) 145, 938, 1004, 1632, 1880
DinI GGCGCC 2 cut(s) 18, 84
DpnI GATC 8 cut(s) 8, 182, 1250, 1809, 1818, 1842, 1854, 2238
DpnII GATC 8 cut(s) 6, 180, 1248, 1807, 1816, 1840, 1852, 2236
DraIII CACNNNGTG 1 cut(s) 1669
DrdI GACNNNNNNGTC 2 cut(s) 41, 124
DriI GACNNNNNGTC 1 cut(s) 58
DseDI GACNNNNNNGTC 2 cut(s) 41, 124
EaeI YGGCCR 1 cut(s) 1234
Eam1104I CTCTTC 3 cut(s) 153, 724, 1520
Eam1105I GACNNNNNGTC 1 cut(s) 58
EarI CTCTTC 3 cut(s) 153, 724, 1520
Eco130I CCWWGG 3 cut(s) 1027, 1155, 1417
Eco147I AGGCCT 1 cut(s) 2283
Eco47I GGWCC 3 cut(s) 968, 1109, 1708
Eco57I CTGAAG 3 cut(s) 257, 634, 1501
Eco81I CCTNAGG 1 cut(s) 2157
EcoO109I RGGNCCY 1 cut(s) 968
EcoRII CCWGG 8 cut(s) 193, 258, 736, 1236, 1527, 1704, 2194, 2257
EcoT14I CCWWGG 3 cut(s) 1027, 1155, 1417
EcoT22I ATGCAT 3 cut(s) 997, 1183, 2042
EgeI GGCGCC 2 cut(s) 18, 84
EheI GGCGCC 2 cut(s) 18, 84
ErhI CCWWGG 3 cut(s) 1027, 1155, 1417
FaqI GGGAC 4 cut(s) 29, 53, 981, 2312
FauI CCCGC 2 cut(s) 130, 219
FblI GTMKAC 3 cut(s) 116, 1442, 1731
Fnu4HI GCNGC 7 cut(s) 20, 57, 497, 786, 849, 870, 1533
FokI GGATG 8 cut(s) 306, 406, 779, 947, 1626, 1637, 1654, 1966
Fsp4HI GCNGC 7 cut(s) 20, 57, 497, 786, 849, 870, 1533
FspBI CTAG 8 cut(s) 363, 603, 911, 1104, 1403, 1446, 1698, 1943
GlaI GCGC 5 cut(s) 18, 84, 229, 434, 715
GluI GCNGC 7 cut(s) 20, 57, 497, 786, 849, 870, 1533
GsaI CCCAGC 2 cut(s) 859, 2122
GsuI CTGGAG 2 cut(s) 810, 828
HaeII RGCGCY 3 cut(s) 20, 86, 717
HaeIII GGCC 2 cut(s) 1236, 2283
HapII CCGG 3 cut(s) 14, 37, 178
HgaI GACGC 4 cut(s) 48, 62, 209, 2309
HhaI GCGC 5 cut(s) 19, 85, 230, 435, 716
Hin1I GRCGYC 6 cut(s) 17, 42, 54, 83, 119, 201
Hin6I GCGC 5 cut(s) 17, 83, 228, 433, 714
HinP1I GCGC 5 cut(s) 17, 83, 228, 433, 714
HincII GTYRAC 4 cut(s) 117, 685, 1122, 1261
HindII GTYRAC 4 cut(s) 117, 685, 1122, 1261
HinfI GANTC 3 cut(s) 1142, 1725, 2342
HpaI GTTAAC 1 cut(s) 1261
HpaII CCGG 3 cut(s) 14, 37, 178
HphI GGTGA 2 cut(s) 533, 1463
Hpy188III TCNNGA 8 cut(s) 377, 508, 677, 878, 911, 1252, 1324, 1976
HpyAV CCTTC 8 cut(s) 281, 340, 822, 1060, 1583, 1843, 1971, 2294
HpyCH4III ACNGT 6 cut(s) 73, 290, 562, 1003, 1126, 1884
HpyCH4IV ACGT 6 cut(s) 42, 111, 119, 171, 661, 2166
HpySE526I ACGT 6 cut(s) 42, 111, 119, 171, 661, 2166
Hsp92I GRCGYC 6 cut(s) 17, 42, 54, 83, 119, 201
HspAI GCGC 5 cut(s) 17, 83, 228, 433, 714
KasI GGCGCC 2 cut(s) 16, 82
KspAI GTTAAC 1 cut(s) 1261
Kzo9I GATC 8 cut(s) 6, 180, 1248, 1807, 1816, 1840, 1852, 2236
LmnI GCTCC 6 cut(s) 465, 626, 791, 809, 1706, 2097
Lsp1109I GCAGC 4 cut(s) 772, 835, 881, 1544
LweI GCATC 9 cut(s) 252, 328, 757, 772, 852, 907, 1694, 1908, 2300
MaeI CTAG 8 cut(s) 363, 603, 911, 1104, 1403, 1446, 1698, 1943
MaeII ACGT 6 cut(s) 42, 111, 119, 171, 661, 2166
MaeIII GTNAC 2 cut(s) 1192, 1363
MalI GATC 8 cut(s) 8, 182, 1250, 1809, 1818, 1842, 1854, 2238
MboI GATC 8 cut(s) 6, 180, 1248, 1807, 1816, 1840, 1852, 2236
MfeI CAATTG 2 cut(s) 1338, 2222
MflI RGATCY 3 cut(s) 180, 1248, 1807
MhlI GDGCHC 5 cut(s) 258, 796, 1050, 1863, 2125
MlsI TGGCCA 1 cut(s) 1236
MluCI AATT 8 cut(s) 422, 1198, 1338, 1503, 1911, 2007, 2066, 2222
MluNI TGGCCA 1 cut(s) 1236
Mly113I GGCGCC 2 cut(s) 17, 83
MmeI TCCRAC 4 cut(s) 69, 1043, 1602, 2149
Mox20I TGGCCA 1 cut(s) 1236
Mph1103I ATGCAT 3 cut(s) 997, 1183, 2042
MroXI GAANNNNTTC 1 cut(s) 1503
MscI TGGCCA 1 cut(s) 1236
MseI TTAA 9 cut(s) 75, 1184, 1260, 1560, 1604, 1910, 1938, 2082, 2132
MslI CAYNNNNRTG 5 cut(s) 229, 291, 660, 1606, 1866
Msp20I TGGCCA 1 cut(s) 1236
MspA1I CMGCKG 2 cut(s) 1190, 1814
MspI CCGG 3 cut(s) 14, 37, 178
MunI CAATTG 2 cut(s) 1338, 2222
Mva1269I GAATGC 4 cut(s) 358, 838, 1183, 2040
MvaI CCWGG 8 cut(s) 195, 260, 738, 1238, 1529, 1706, 2196, 2259
MvnI CGCG 3 cut(s) 59, 230, 2303
NarI GGCGCC 2 cut(s) 17, 83
NciI CCSGG 2 cut(s) 14, 38
NdeII GATC 8 cut(s) 6, 180, 1248, 1807, 1816, 1840, 1852, 2236
NheI GCTAGC 2 cut(s) 602, 1697
NlaIV GGNNCC 8 cut(s) 18, 84, 191, 461, 590, 628, 969, 1959
NmuCI GTSAC 1 cut(s) 1363
NsiI ATGCAT 3 cut(s) 997, 1183, 2042
NspI RCATGY 5 cut(s) 964, 1390, 1738, 1871, 2023
NspV TTCGAA 1 cut(s) 1286
PaeI GCATGC 2 cut(s) 964, 1871
PaqCI CACCTGC 1 cut(s) 367
PceI AGGCCT 1 cut(s) 2283
PciI ACATGT 1 cut(s) 1386
PcsI WCGNNNNNNNCGW 4 cut(s) 48, 108, 122, 125
PctI GAATGC 4 cut(s) 358, 838, 1183, 2040
PdmI GAANNNNTTC 1 cut(s) 1503
PfeI GAWTC 3 cut(s) 1142, 1725, 2342
PflFI GACNNNGTC 1 cut(s) 121
PfoI TCCNGGA 1 cut(s) 736
PkrI GCNGC 7 cut(s) 21, 58, 498, 787, 850, 871, 1534
PluTI GGCGCC 2 cut(s) 20, 86
Ppu21I YACGTR 1 cut(s) 662
PpuMI RGGWCCY 1 cut(s) 968
PscI ACATGT 1 cut(s) 1386
PshBI ATTAAT 1 cut(s) 1604
Psp1406I AACGTT 1 cut(s) 2166
Psp5II RGGWCCY 1 cut(s) 968
Psp6I CCWGG 8 cut(s) 193, 258, 736, 1236, 1527, 1704, 2194, 2257
PspFI CCCAGC 2 cut(s) 855, 2118
PspGI CCWGG 8 cut(s) 193, 258, 736, 1236, 1527, 1704, 2194, 2257
PspN4I GGNNCC 8 cut(s) 18, 84, 191, 461, 590, 628, 969, 1959
PspPI GGNCC 3 cut(s) 968, 1109, 1708
PspPPI RGGWCCY 1 cut(s) 968
PsrI GAACNNNNNNTAC 2 cut(s) 717, 749
PstNI CAGNNNCTG 3 cut(s) 1528, 2195, 2264
PsuI RGATCY 3 cut(s) 180, 1248, 1807
PsyI GACNNNGTC 1 cut(s) 121
PvuII CAGCTG 2 cut(s) 1190, 1814
RsaI GTAC 5 cut(s) 146, 939, 1005, 1633, 1881
RsaNI GTAC 5 cut(s) 145, 938, 1004, 1632, 1880
RseI CAYNNNNRTG 5 cut(s) 229, 291, 660, 1606, 1866
SalI GTCGAC 1 cut(s) 115
SaqAI TTAA 9 cut(s) 75, 1184, 1260, 1560, 1604, 1910, 1938, 2082, 2132
SatI GCNGC 7 cut(s) 20, 57, 497, 786, 849, 870, 1533
Sau3AI GATC 8 cut(s) 6, 180, 1248, 1807, 1816, 1840, 1852, 2236
Sau96I GGNCC 3 cut(s) 968, 1109, 1708
SduI GDGCHC 5 cut(s) 258, 796, 1050, 1863, 2125
SfaNI GCATC 9 cut(s) 252, 328, 757, 772, 852, 907, 1694, 1908, 2300
SfcI CTRYAG 4 cut(s) 184, 558, 2213, 2231
SfoI GGCGCC 2 cut(s) 18, 84
SfuI TTCGAA 1 cut(s) 1286
SinI GGWCC 3 cut(s) 968, 1109, 1708
SmiMI CAYNNNNRTG 5 cut(s) 229, 291, 660, 1606, 1866
SmlI CTYRAG 1 cut(s) 878
SmoI CTYRAG 1 cut(s) 878
SphI GCATGC 2 cut(s) 964, 1871
Sse9I AATT 8 cut(s) 422, 1198, 1338, 1503, 1911, 2007, 2066, 2222
SseBI AGGCCT 1 cut(s) 2283
SspDI GGCGCC 2 cut(s) 16, 82
SspI AATATT 1 cut(s) 1041
SspMI CTAG 8 cut(s) 363, 603, 911, 1104, 1403, 1446, 1698, 1943
StuI AGGCCT 1 cut(s) 2283
StyI CCWWGG 3 cut(s) 1027, 1155, 1417
TaaI ACNGT 6 cut(s) 73, 290, 562, 1003, 1126, 1884
TaiI ACGT 6 cut(s) 45, 114, 122, 174, 664, 2169
TaqI TCGA 4 cut(s) 116, 1286, 1768, 2278
TasI AATT 8 cut(s) 422, 1198, 1338, 1503, 1911, 2007, 2066, 2222
TatI WGTACW 2 cut(s) 1003, 1631
TauI GCSGC 3 cut(s) 22, 59, 499
TfiI GAWTC 3 cut(s) 1142, 1725, 2342
Tru1I TTAA 9 cut(s) 75, 1184, 1260, 1560, 1604, 1910, 1938, 2082, 2132
Tru9I TTAA 9 cut(s) 75, 1184, 1260, 1560, 1604, 1910, 1938, 2082, 2132
TscAI CASTG 3 cut(s) 281, 1144, 2332
TseFI GTSAC 1 cut(s) 1363
TseI GCWGC 4 cut(s) 785, 848, 869, 1532
Tsp45I GTSAC 1 cut(s) 1363
TspDTI ATGAA 8 cut(s) 416, 466, 604, 711, 968, 1423, 2283, 2346
TspGWI ACGGA 4 cut(s) 442, 1826, 1879, 2084
TspRI CASTG 3 cut(s) 281, 1144, 2332
Tth111I GACNNNGTC 1 cut(s) 121
VneI GTGCAC 2 cut(s) 254, 1859
VpaK11BI GGWCC 3 cut(s) 968, 1109, 1708
VspI ATTAAT 1 cut(s) 1604
XapI RAATTY 2 cut(s) 1198, 2007
XbaI TCTAGA 1 cut(s) 910
XceI RCATGY 5 cut(s) 964, 1390, 1738, 1871, 2023
XmiI GTMKAC 3 cut(s) 116, 1442, 1731
XmnI GAANNNNTTC 1 cut(s) 1503
XspI CTAG 8 cut(s) 363, 603, 911, 1104, 1403, 1446, 1698, 1943
ZraI GACGTC 2 cut(s) 43, 120
Zsp2I ATGCAT 3 cut(s) 997, 1183, 2042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.