Rorug05G0565000

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0009109
Physical Location & Seq
Forward (+)
75686447 .. 75693493
7047 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0565000.1

Sequence Viewer

Length: 1599 bp
ATGGCACTTCAAGGCACTCTCATCTCTGCAATCATACTTGTTTTTAGCTGTGCAGTGGCAACTAGTATAGCTACCGCCCCAAGCCATTACAACGTTGCTTCCATCAACCGGAGCACCTTTCCGGCAGGTTTCATATTTGGGACAGCTTCTTCAGCTTACCAGTTCGAAGGTGCTGCAAAAGAAGATGGAAGAGGACCAAGCATATGGGACACATACACTCACAAAATTCCAGATAAGATAAAAGACGGCAGCAATGGAGATGTCGCTATTGATGCATATCACCGCTACAAGGAGGATGTTGGCATCATGAAAAATATGGGGTTTGATGCTTACAGATTCTCCATCTCGTGGTCTAGATTGTTACCAAATGGAACGCTAAGAGGGGGTGTGAATAAGGAAGGAATCAAATATTACAACAACCTCATAAACGAGCTTCTAGCCAATGGTCTAAAGCCCTTTGTCACACTCTTCCACTGGGATCTTCCCCAAGCTTTAGAAGATGAATACGGTGGCTTCTTAAGCCCTCAAATTGTAAACCATTTCCAAGACTACGCAGAGCTATGCTTCAAGGAATTTGGTGATAGAGTTAAGGACTGGATCACATTAAACGAGCCCTGGAGCTACGCCAATGGTGGTTATGTAATTGGGACGTTTGCACCATGCCGATGTTCTGAATGGCAAAACCTAAATTGCACCTGTGGAAATTCAGGAACAGAGCCATATTTGGTCTCACACTACCAGCTTCTAGCTCACGCAGCAGCCGTAAAGTTATACAAGGAGAAGTATCAGGCTGATCAGAAGGGTGTGATAGGGATAACAATACTGTCACACTGGTTTGTGCCCTTTTCTGATGCCAAGCACCATGAACAAGCTGCGTGTTATCCGTCACACTGGTTTGTGCCCTTTTCTGATGCCAAGCACCATGAAGAAGCTGCTCTACGAGCATTGGATTTTATGTTTGGATGGTATATGGATCCCTTAACAAACGGTGAATATCCGCACAGCATGAGATCTCTTGTCGGAGATCGATTACCCAAGTTCACTAAAGAGCAATCCGAGATGTTAAAAGGTTCATTTGATTTTCTGGGATTGAATTACTACACCGCTAACTATGCTACTTATGCACCTCATCTCAAGAATGCTGCAAACCCTAGCTACTTTACAGATGCTGTCGCTACTGTTTCCACCGAGCGTAATGGTATTCCTATTGGTCAAAAGGCTGCTTCAGATTGGCTCTATGTTTATCCAGAAGGAATTCGAGAACTATTGCTTTACACGAAGGAAAAGTACAATAATCCACTTATTTACATCACTGAAAATGGAAGGGATGAGCACAATGATGCCAAATTATCACTTGAGGAAGCCCTAGCCGATACTCACCGAATCGACTTTTACTATCGACATCTTTATTATCTTCATGAAGCGATCAAGGATGGCGTTAACGTGAAGGGATATTTTGCTTGGTCTTTGTTTGACAACTTCGAATGGAATATGGGTTACAGTGTTAGGTTTGGCATCAACTATGTGGATTACAATGATGGGCTCAAAAGATACCCTAAACTCTCAGCACACTGGTTCAAGAATTTTCTTGAAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000209 GO:0001101 GO:0002020 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006508 GO:0006511 GO:0006629 GO:0006720 GO:0006725 GO:0006807 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009414 GO:0009415 GO:0009628 GO:0009698 GO:0009699 GO:0009893 GO:0009987 GO:0010025 GO:0010033 GO:0010035 GO:0010143 GO:0010166 GO:0010243 GO:0010345 GO:0010498 GO:0012505 GO:0016020 GO:0016021 GO:0016567 GO:0016740 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019787 GO:0019899 GO:0019941 GO:0030163 GO:0030176 GO:0030433 GO:0031224 GO:0031227 GO:0031624 GO:0031984 GO:0032446 GO:0032501 GO:0032502 GO:0033554 GO:0034976 GO:0036211 GO:0036503 GO:0042175 GO:0042221 GO:0042335 GO:0043085 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044390 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0048518 GO:0048856 GO:0050789 GO:0050790 GO:0050896 GO:0051341 GO:0051353 GO:0051603 GO:0051716 GO:0065007 GO:0065009 GO:0070647 GO:0070936 GO:0071704 GO:0098827 GO:0140096 GO:1900490 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901568 GO:1901570 GO:1901575 GO:1901576 GO:1901698 GO:1901700 GO:1990381 GO:2001215
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
RINGv

Protein Analysis

532

Amino Acids

60.64

Weight (kDa)

5.92

Isoelectric Point (pI)

27.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 37 - 291 9e-97 Glycosyl hydrolase family 1
Glyco_hydro_1 PF00232 295 - 531 3.8e-60 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 116
AccB7I CCANNNNNTGG 1 cut(s) 348
AciI CCGC 4 cut(s) 75, 283, 998, 1104
AclI AACGTT 1 cut(s) 93
AclWI GGATC 4 cut(s) 486, 605, 968, 981
AcsI RAATTY 5 cut(s) 225, 572, 703, 1254, 1582
AcuI CTGAAG 2 cut(s) 135, 1209
AfaI GTAC 1 cut(s) 1289
AfiI CCNNNNNNNGG 3 cut(s) 108, 289, 348
AflII CTTAAG 1 cut(s) 517
AgsI TTSAA 5 cut(s) 11, 568, 1093, 1579, 1592
AhlI ACTAGT 1 cut(s) 62
AjnI CCWGG 1 cut(s) 614
Alw21I GWGCWC 2 cut(s) 116, 1335
Alw26I GTCTC 1 cut(s) 733
AlwI GGATC 4 cut(s) 486, 605, 968, 981
AlwNI CAGNNNCTG 1 cut(s) 1169
ApeKI GCWGC 8 cut(s) 173, 249, 755, 758, 872, 932, 1142, 1220
ApoI RAATTY 5 cut(s) 225, 572, 703, 1254, 1582
Asp700I GAANNNNTTC 1 cut(s) 1254
AspS9I GGNCC 1 cut(s) 194
AsuHPI GGTGA 4 cut(s) 272, 590, 1001, 1370
AsuII TTCGAA 2 cut(s) 165, 1482
AvaII GGWCC 1 cut(s) 194
BaeGI GKGCMC 2 cut(s) 843, 903
BamHI GGATCC 1 cut(s) 973
BanII GRGCYC 2 cut(s) 615, 1545
BarI GAAGNNNNNNTAC 4 cut(s) 921, 953, 1271, 1303
BauI CACGAG 1 cut(s) 346
Bbv12I GWGCWC 2 cut(s) 116, 1335
BbvI GCAGC 8 cut(s) 160, 261, 767, 770, 859, 919, 1129, 1207
BccI CCATC 6 cut(s) 110, 179, 350, 957, 1427, 1532
BceAI ACGGC 2 cut(s) 262, 746
BcgI CGANNNNNNTGC 2 cut(s) 155, 189
BciT130I CCWGG 1 cut(s) 616
BclI TGATCA 1 cut(s) 793
BcoDI GTCTC 1 cut(s) 733
BcuI ACTAGT 1 cut(s) 62
BfaI CTAG 6 cut(s) 63, 354, 437, 746, 1152, 1367
BfrI CTTAAG 1 cut(s) 517
BfuAI ACCTGC 1 cut(s) 116
BglII AGATCT 1 cut(s) 1010
BisI GCNGC 8 cut(s) 174, 250, 756, 759, 873, 933, 1143, 1221
BlsI GCNGC 8 cut(s) 175, 251, 757, 760, 874, 934, 1144, 1222
Bme1390I CCNGG 1 cut(s) 616
Bme18I GGWCC 1 cut(s) 194
BmgT120I GGNCC 1 cut(s) 194
BmiI GGNNCC 1 cut(s) 975
BmrFI CCNGG 1 cut(s) 616
BmrI ACTGGG 1 cut(s) 484
BmsI GCATC 8 cut(s) 262, 312, 316, 841, 901, 1156, 1330, 1524
BmuI ACTGGG 1 cut(s) 484
BpmI CTGGAG 1 cut(s) 637
Bpu14I TTCGAA 2 cut(s) 165, 1482
BpuEI CTTGAG 2 cut(s) 1118, 1376
Bsa29I ATCGAT 1 cut(s) 1027
BsaBI GATNNNNATC 1 cut(s) 276
BsaI GGTCTC 1 cut(s) 733
BsaJI CCNNGG 1 cut(s) 614
BsaWI WCCGGW 1 cut(s) 108
BsaXI ACNNNNNCTCC 2 cut(s) 323, 353
Bsc4I CCNNNNNNNGG 3 cut(s) 108, 289, 348
Bse1I ACTGG 6 cut(s) 160, 479, 599, 836, 896, 1577
Bse3DI GCAATG 1 cut(s) 259
Bse8I GATNNNNATC 1 cut(s) 276
BseBI CCWGG 1 cut(s) 616
BseCI ATCGAT 1 cut(s) 1027
BseDI CCNNGG 1 cut(s) 614
BseGI GGATG 4 cut(s) 301, 968, 1333, 1438
BseJI GATNNNNATC 1 cut(s) 276
BseLI CCNNNNNNNGG 3 cut(s) 108, 289, 348
BseMI GCAATG 1 cut(s) 259
BseMII CTCAG 1 cut(s) 1578
BseNI ACTGG 6 cut(s) 160, 479, 599, 836, 896, 1577
BseSI GKGCMC 2 cut(s) 843, 903
BseXI GCAGC 8 cut(s) 160, 261, 767, 770, 859, 919, 1129, 1207
BsgI GTGCAG 1 cut(s) 72
BshVI ATCGAT 1 cut(s) 1027
BsiHKAI GWGCWC 2 cut(s) 116, 1335
BsiSI CCGG 2 cut(s) 109, 122
BslFI GGGAC 3 cut(s) 154, 221, 661
BslI CCNNNNNNNGG 3 cut(s) 108, 289, 348
BsmAI GTCTC 1 cut(s) 733
BsmFI GGGAC 3 cut(s) 154, 221, 661
BsmI GAATGC 1 cut(s) 1144
Bso31I GGTCTC 1 cut(s) 733
Bsp119I TTCGAA 2 cut(s) 165, 1482
Bsp1286I GDGCHC 6 cut(s) 116, 615, 843, 903, 1335, 1545
Bsp143I GATC 7 cut(s) 478, 597, 793, 973, 1010, 1024, 1425
BspACI CCGC 4 cut(s) 75, 283, 998, 1104
BspCNI CTCAG 1 cut(s) 1577
BspDI ATCGAT 1 cut(s) 1027
BspHI TCATGA 2 cut(s) 306, 1417
BspLI GGNNCC 1 cut(s) 975
BspMI ACCTGC 1 cut(s) 116
BspPI GGATC 4 cut(s) 486, 605, 968, 981
BspT104I TTCGAA 2 cut(s) 165, 1482
BspTI CTTAAG 1 cut(s) 517
BspTNI GGTCTC 1 cut(s) 733
BsrDI GCAATG 1 cut(s) 259
BsrI ACTGG 6 cut(s) 160, 479, 599, 836, 896, 1577
BssECI CCNNGG 1 cut(s) 614
BssMI GATC 7 cut(s) 478, 597, 793, 973, 1010, 1024, 1425
BssSI CACGAG 1 cut(s) 346
Bst2BI CACGAG 1 cut(s) 346
Bst2UI CCWGG 1 cut(s) 616
Bst4CI ACNGT 5 cut(s) 509, 825, 989, 1180, 1502
Bst6I CTCTTC 2 cut(s) 184, 473
BstAFI CTTAAG 1 cut(s) 517
BstBI TTCGAA 2 cut(s) 165, 1482
BstDEI CTNAG 2 cut(s) 377, 1564
BstF5I GGATG 4 cut(s) 301, 968, 1333, 1438
BstKTI GATC 7 cut(s) 481, 600, 796, 976, 1013, 1027, 1428
BstMAI GTCTC 1 cut(s) 733
BstMBI GATC 7 cut(s) 478, 597, 793, 973, 1010, 1024, 1425
BstMWI GCNNNNNNNGC 7 cut(s) 152, 272, 519, 755, 941, 1112, 1121
BstNI CCWGG 1 cut(s) 616
BstSCI CCNGG 1 cut(s) 614
BstSLI GKGCMC 2 cut(s) 843, 903
BstV1I GCAGC 8 cut(s) 160, 261, 767, 770, 859, 919, 1129, 1207
BstX2I RGATCY 3 cut(s) 478, 973, 1010
BstXI CCANNNNNNTGG 1 cut(s) 204
BstYI RGATCY 3 cut(s) 478, 973, 1010
Bsu15I ATCGAT 1 cut(s) 1027
BsuTUI ATCGAT 1 cut(s) 1027
BtsCI GGATG 4 cut(s) 301, 968, 1333, 1438
BtsI GCAGTG 1 cut(s) 60
BtsIMutI CAGTG 7 cut(s) 60, 472, 829, 889, 1311, 1507, 1570
BveI ACCTGC 1 cut(s) 116
CaiI CAGNNNCTG 1 cut(s) 1169
CciI TCATGA 2 cut(s) 306, 1417
Cfr13I GGNCC 1 cut(s) 194
ClaI ATCGAT 1 cut(s) 1027
Csp6I GTAC 1 cut(s) 1288
CviAII CATG 6 cut(s) 307, 660, 863, 923, 1006, 1418
CviQI GTAC 1 cut(s) 1288
DdeI CTNAG 2 cut(s) 377, 1564
DpnI GATC 7 cut(s) 480, 599, 795, 975, 1012, 1026, 1427
DpnII GATC 7 cut(s) 478, 597, 793, 973, 1010, 1024, 1425
Eam1104I CTCTTC 2 cut(s) 184, 473
EarI CTCTTC 2 cut(s) 184, 473
Eco24I GRGCYC 2 cut(s) 615, 1545
Eco31I GGTCTC 1 cut(s) 733
Eco47I GGWCC 1 cut(s) 194
Eco57I CTGAAG 2 cut(s) 135, 1209
EcoRI GAATTC 1 cut(s) 1254
EcoRII CCWGG 1 cut(s) 614
EcoT22I ATGCAT 1 cut(s) 277
EcoT38I GRGCYC 2 cut(s) 615, 1545
FaeI CATG 6 cut(s) 310, 663, 866, 926, 1009, 1421
FaqI GGGAC 3 cut(s) 154, 221, 661
FatI CATG 6 cut(s) 306, 659, 862, 922, 1005, 1417
FauNDI CATATG 1 cut(s) 203
FbaI TGATCA 1 cut(s) 793
Fnu4HI GCNGC 8 cut(s) 174, 250, 756, 759, 873, 933, 1143, 1221
FokI GGATG 4 cut(s) 308, 975, 1340, 1445
FriOI GRGCYC 2 cut(s) 615, 1545
Fsp4HI GCNGC 8 cut(s) 174, 250, 756, 759, 873, 933, 1143, 1221
FspBI CTAG 6 cut(s) 63, 354, 437, 746, 1152, 1367
GluI GCNGC 8 cut(s) 174, 250, 756, 759, 873, 933, 1143, 1221
GsuI CTGGAG 1 cut(s) 637
HapII CCGG 2 cut(s) 109, 122
Hin1II CATG 6 cut(s) 310, 663, 866, 926, 1009, 1421
HincII GTYRAC 1 cut(s) 1441
HindII GTYRAC 1 cut(s) 1441
HindIII AAGCTT 1 cut(s) 489
HinfI GANTC 3 cut(s) 336, 402, 1383
HpaI GTTAAC 1 cut(s) 1441
HpaII CCGG 2 cut(s) 109, 122
HphI GGTGA 4 cut(s) 272, 590, 1001, 1370
Hpy166II GTNNAC 3 cut(s) 535, 1041, 1441
Hpy188I TCNGA 7 cut(s) 673, 798, 850, 910, 1022, 1057, 1228
Hpy8I GTNNAC 3 cut(s) 535, 1041, 1441
HpyAV CCTTC 7 cut(s) 161, 392, 793, 1244, 1273, 1317, 1441
HpyCH4III ACNGT 5 cut(s) 509, 825, 989, 1180, 1502
HpyCH4IV ACGT 3 cut(s) 93, 650, 1443
HpyCH4V TGCA 8 cut(s) 29, 53, 176, 275, 656, 693, 1124, 1145
HpyF10VI GCNNNNNNNGC 7 cut(s) 152, 272, 519, 755, 941, 1112, 1121
HpyF3I CTNAG 2 cut(s) 377, 1564
HpySE526I ACGT 3 cut(s) 93, 650, 1443
Hsp92II CATG 6 cut(s) 310, 663, 866, 926, 1009, 1421
Ksp22I TGATCA 1 cut(s) 793
KspAI GTTAAC 1 cut(s) 1441
Kzo9I GATC 7 cut(s) 478, 597, 793, 973, 1010, 1024, 1425
LmnI GCTCC 2 cut(s) 111, 618
Lsp1109I GCAGC 8 cut(s) 160, 261, 767, 770, 859, 919, 1129, 1207
LweI GCATC 8 cut(s) 262, 312, 316, 841, 901, 1156, 1330, 1524
MaeI CTAG 6 cut(s) 63, 354, 437, 746, 1152, 1367
MaeII ACGT 3 cut(s) 93, 650, 1443
MaeIII GTNAC 5 cut(s) 360, 460, 825, 885, 1496
MalI GATC 7 cut(s) 480, 599, 795, 975, 1012, 1026, 1427
MboI GATC 7 cut(s) 478, 597, 793, 973, 1010, 1024, 1425
MboII GAAGA 8 cut(s) 141, 194, 201, 460, 473, 509, 938, 1406
MflI RGATCY 3 cut(s) 478, 973, 1010
MhlI GDGCHC 6 cut(s) 116, 615, 843, 903, 1335, 1545
MmeI TCCRAC 1 cut(s) 1000
MnlI CCTC 7 cut(s) 185, 286, 374, 431, 534, 1137, 1351
Mph1103I ATGCAT 1 cut(s) 277
MroXI GAANNNNTTC 1 cut(s) 1254
MseI TTAA 6 cut(s) 518, 588, 605, 980, 1064, 1440
MslI CAYNNNNRTG 2 cut(s) 664, 1338
MspCI CTTAAG 1 cut(s) 517
MspI CCGG 2 cut(s) 109, 122
MspR9I CCNGG 1 cut(s) 616
Mva1269I GAATGC 1 cut(s) 1144
MvaI CCWGG 1 cut(s) 616
MwoI GCNNNNNNNGC 7 cut(s) 152, 272, 519, 755, 941, 1112, 1121
NdeI CATATG 1 cut(s) 203
NdeII GATC 7 cut(s) 478, 597, 793, 973, 1010, 1024, 1425
NlaIII CATG 6 cut(s) 310, 663, 866, 926, 1009, 1421
NlaIV GGNNCC 1 cut(s) 975
NmuCI GTSAC 3 cut(s) 460, 825, 885
NsiI ATGCAT 1 cut(s) 277
NspV TTCGAA 2 cut(s) 165, 1482
PagI TCATGA 2 cut(s) 306, 1417
PcsI WCGNNNNNNNCGW 1 cut(s) 759
PctI GAATGC 1 cut(s) 1144
PdmI GAANNNNTTC 1 cut(s) 1254
PfeI GAWTC 3 cut(s) 336, 402, 1383
PflMI CCANNNNNTGG 1 cut(s) 348
PkrI GCNGC 8 cut(s) 175, 251, 757, 760, 874, 934, 1144, 1222
Psp1406I AACGTT 1 cut(s) 93
Psp6I CCWGG 1 cut(s) 614
PspGI CCWGG 1 cut(s) 614
PspN4I GGNNCC 1 cut(s) 975
PspPI GGNCC 1 cut(s) 194
PstNI CAGNNNCTG 1 cut(s) 1169
PsuI RGATCY 3 cut(s) 478, 973, 1010
RsaI GTAC 1 cut(s) 1289
RsaNI GTAC 1 cut(s) 1288
RseI CAYNNNNRTG 2 cut(s) 664, 1338
SaqAI TTAA 6 cut(s) 518, 588, 605, 980, 1064, 1440
SatI GCNGC 8 cut(s) 174, 250, 756, 759, 873, 933, 1143, 1221
Sau3AI GATC 7 cut(s) 478, 597, 793, 973, 1010, 1024, 1425
Sau96I GGNCC 1 cut(s) 194
ScrFI CCNGG 1 cut(s) 616
SduI GDGCHC 6 cut(s) 116, 615, 843, 903, 1335, 1545
SfaNI GCATC 8 cut(s) 262, 312, 316, 841, 901, 1156, 1330, 1524
SfuI TTCGAA 2 cut(s) 165, 1482
SinI GGWCC 1 cut(s) 194
SmiMI CAYNNNNRTG 2 cut(s) 664, 1338
SmlI CTYRAG 3 cut(s) 517, 1133, 1355
SmoI CTYRAG 3 cut(s) 517, 1133, 1355
SpeI ACTAGT 1 cut(s) 62
SsiI CCGC 4 cut(s) 75, 283, 998, 1104
SspI AATATT 1 cut(s) 410
SspMI CTAG 6 cut(s) 63, 354, 437, 746, 1152, 1367
StyD4I CCNGG 1 cut(s) 614
TaaI ACNGT 5 cut(s) 509, 825, 989, 1180, 1502
TaiI ACGT 3 cut(s) 96, 653, 1446
TaqI TCGA 6 cut(s) 165, 1027, 1258, 1386, 1399, 1482
TatI WGTACW 1 cut(s) 1287
TfiI GAWTC 3 cut(s) 336, 402, 1383
Tru1I TTAA 6 cut(s) 518, 588, 605, 980, 1064, 1440
Tru9I TTAA 6 cut(s) 518, 588, 605, 980, 1064, 1440
TscAI CASTG 7 cut(s) 60, 479, 836, 896, 1318, 1507, 1577
TseFI GTSAC 3 cut(s) 460, 825, 885
TseI GCWGC 8 cut(s) 173, 249, 755, 758, 872, 932, 1142, 1220
Tsp45I GTSAC 3 cut(s) 460, 825, 885
TspDTI ATGAA 8 cut(s) 121, 323, 516, 879, 939, 1062, 1406, 1434
TspGWI ACGGA 1 cut(s) 873
TspRI CASTG 7 cut(s) 60, 479, 836, 896, 1318, 1507, 1577
Van91I CCANNNNNTGG 1 cut(s) 348
Vha464I CTTAAG 1 cut(s) 517
VpaK11BI GGWCC 1 cut(s) 194
XapI RAATTY 5 cut(s) 225, 572, 703, 1254, 1582
XbaI TCTAGA 1 cut(s) 353
XmnI GAANNNNTTC 1 cut(s) 1254
XspI CTAG 6 cut(s) 63, 354, 437, 746, 1152, 1367
Zsp2I ATGCAT 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.