Rorug05G0564900

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0009109
Physical Location & Seq
Forward (+)
75677196 .. 75683383
6188 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0564900.1

Sequence Viewer

Length: 1548 bp
ATGGCAATCCAGGGGTCTTTGCTTTTGGCCATAGCGTTTCTAGTACTTGGCTTTGGATTTAGAAATGGTAAGGCCAGTAATACACCCCCTGCTCAATATGACACTGCTTTCCTCAACAGGACTAGTTTCCCAGCAGGTTTCATATTTGGGACAGCTTCATCATCTTACCAGTATGAAGGTGCTGCTCATTGTGGTGGCAGGGGACCAAGCATATGGGATAACTATACCCACAGATATCCTGAAAAGATCAAGGATGGCAGCAATGGAGATGTGGCTAGTGATGAATATCACCGCTATAAGGAAGATGTGGGGATTATGAAGAACATGGAGTTGGATGCATATCGGTTCTCTATCTCATGGTCCAGATTGTTACCAAATGGAAAGCTAACTGGGGGCGTGAACAAGGAAGGAGTCAAATACTACAACAATCTCATCAATGAACTCTTACGCAATGGTCTAAAGCCATTTGTAACCCTCTTCCACTGGGATCTTCCCCAGACTTTAGAAGATGAATATGGCGGTTTCTTAAGCCCTCTCATTGTCAATCATTTTAGGGACTATGCAGAGCTTTGTTACAAGGAATTTGGTGATAGGGTAAAGCACTGGATCACTTTTAATGAGCCATATAGTTATAGTGCTGGTGGTTATGCAGTGGCAATTTTGGCACCAGGACGCTGTTCTGATTGGCAGGAACTAAATTGCACCGGTGGGAATTCGGGTACTGAACCATACTTGGTGGCACACAACCAGCTCCTTGCTCATACAGCTGCAGTAACATTATACAAGCAGAAATATCAGTCATCTCAAAAGGGATTGATAGGGATTACTCTAGTGTCAAACTGGTTTGTGCCGGTTTCTGAGGCAGAGCACCATAAAAATGCTGCACTAAGAGCTCTAGATTATATGTTTGGATGGTTTATGGACCCCTTGACAAATGGTGACTATCCACACAGCATGAGATCTCTTGTTGGAAACCGATTACCCAAATTCACAAAGGAGCAATCCAAGTTGCTAATTGGATCATTTGATTTTCTGGGATTGAATTACTACACAGCTAATTATGCAGCAGATGCACCACACTATAATTCTGTCAATGCAAGCTACTTGACAGACGCTCATGCTACTCTTTCCTATGTGCTTAATGGAATTCCCATTGGACCAAAGGCTGCTTCGGATTGGCTATACGTTTATCCCAGAGGAATTCGAGATCTTTTGCTCTACACGAAGACAAAGTATAATGATCCACTCATATACGTTACTGAGAATGGTATTGATGAGTTCAATGATCCCAAATTAACACTTGAAGTATCCCTGAACGACACCCAGAGAGTTGACTACTACTATCGCCACCTCTATTACCTTCAAAGAGCAATCAAGGATGGTGTGAATGTTAAGGGATACTTTGCATGGTCATTGCTAGACAACTTTGAATGGAATTCTGGTTACACTGTCCGATTTGGTATCAACTATGTAGATTACAAAAATGGGCAGAATAGGCACCCAAAACTTTCTGCACACTGGTTCAAACGTTTCCTCAAGAAAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000209 GO:0001101 GO:0002020 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006508 GO:0006511 GO:0006629 GO:0006720 GO:0006725 GO:0006807 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009414 GO:0009415 GO:0009628 GO:0009698 GO:0009699 GO:0009893 GO:0009987 GO:0010025 GO:0010033 GO:0010035 GO:0010143 GO:0010166 GO:0010243 GO:0010345 GO:0010498 GO:0012505 GO:0016020 GO:0016021 GO:0016567 GO:0016740 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019787 GO:0019899 GO:0019941 GO:0030163 GO:0030176 GO:0030433 GO:0031224 GO:0031227 GO:0031624 GO:0031984 GO:0032446 GO:0032501 GO:0032502 GO:0033554 GO:0034976 GO:0036211 GO:0036503 GO:0042175 GO:0042221 GO:0042335 GO:0043085 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044390 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0048518 GO:0048856 GO:0050789 GO:0050790 GO:0050896 GO:0051341 GO:0051353 GO:0051603 GO:0051716 GO:0065007 GO:0065009 GO:0070647 GO:0070936 GO:0071704 GO:0098827 GO:0140096 GO:1900490 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901568 GO:1901570 GO:1901575 GO:1901576 GO:1901698 GO:1901700 GO:1990381 GO:2001215
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
RINGv

Protein Analysis

515

Amino Acids

58.68

Weight (kDa)

8.32

Isoelectric Point (pI)

22.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 41 - 514 2.5e-155 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 125
AccB1I GGYRCC 2 cut(s) 664, 1497
AciI CCGC 2 cut(s) 292, 519
AclI AACGTT 1 cut(s) 1528
AclWI GGATC 5 cut(s) 495, 614, 1027, 1235, 1280
AcoI YGGCCR 1 cut(s) 27
AcsI RAATTY 6 cut(s) 581, 712, 986, 1146, 1200, 1435
AfaI GTAC 2 cut(s) 45, 721
AfiI CCNNNNNNNGG 1 cut(s) 298
AflII CTTAAG 1 cut(s) 526
AgeI ACCGGT 1 cut(s) 704
AgsI TTSAA 6 cut(s) 1042, 1282, 1304, 1364, 1430, 1525
AhlI ACTAGT 1 cut(s) 122
AjnI CCWGG 2 cut(s) 9, 667
AjuI GAANNNNNNNTTGG 4 cut(s) 314, 346, 1153, 1185
AluBI AGCT 8 cut(s) 155, 385, 568, 751, 767, 893, 1055, 1101
AluI AGCT 8 cut(s) 155, 385, 568, 751, 767, 893, 1055, 1101
Alw21I GWGCWC 2 cut(s) 870, 895
AlwI GGATC 5 cut(s) 495, 614, 1027, 1235, 1280
AoxI GGCC 2 cut(s) 27, 72
ApeKI GCWGC 6 cut(s) 182, 258, 767, 881, 1064, 1166
ApoI RAATTY 6 cut(s) 581, 712, 986, 1146, 1200, 1435
AsiGI ACCGGT 1 cut(s) 704
AspS9I GGNCC 4 cut(s) 203, 360, 922, 1157
AsuHPI GGTGA 3 cut(s) 281, 599, 950
AvaII GGWCC 4 cut(s) 203, 360, 922, 1157
BalI TGGCCA 1 cut(s) 29
BanI GGYRCC 2 cut(s) 664, 1497
BanII GRGCYC 1 cut(s) 895
BbsI GAAGAC 1 cut(s) 1232
Bbv12I GWGCWC 2 cut(s) 870, 895
BbvI GCAGC 6 cut(s) 169, 270, 754, 868, 1076, 1153
BccI CCATC 3 cut(s) 248, 906, 1373
BciT130I CCWGG 2 cut(s) 11, 669
BciVI GTATCC 2 cut(s) 1318, 1391
BcuI ACTAGT 1 cut(s) 122
BfaI CTAG 6 cut(s) 41, 123, 276, 830, 896, 1418
BfmI CTRYAG 1 cut(s) 768
BfrI CTTAAG 1 cut(s) 526
BfuAI ACCTGC 1 cut(s) 125
BfuI GTATCC 2 cut(s) 1318, 1391
BglII AGATCT 2 cut(s) 959, 1207
BisI GCNGC 6 cut(s) 183, 259, 768, 882, 1065, 1167
BlsI GCNGC 6 cut(s) 184, 260, 769, 883, 1066, 1168
BmcAI AGTACT 1 cut(s) 45
Bme1390I CCNGG 2 cut(s) 11, 669
Bme18I GGWCC 4 cut(s) 203, 360, 922, 1157
BmgT120I GGNCC 4 cut(s) 203, 360, 922, 1157
BmiI GGNNCC 4 cut(s) 204, 666, 924, 1499
BmrFI CCNGG 2 cut(s) 11, 669
BmrI ACTGGG 2 cut(s) 399, 493
BmsI GCATC 2 cut(s) 325, 1060
BmuI ACTGGG 2 cut(s) 399, 493
BpiI GAAGAC 1 cut(s) 1232
BpuEI CTTGAG 1 cut(s) 1520
BsaBI GATNNNNATC 2 cut(s) 285, 339
BsaJI CCNNGG 1 cut(s) 10
BsaWI WCCGGW 1 cut(s) 704
Bsc4I CCNNNNNNNGG 1 cut(s) 298
Bse118I RCCGGY 2 cut(s) 704, 850
Bse1I ACTGG 7 cut(s) 75, 169, 394, 488, 608, 845, 1523
Bse3DI GCAATG 3 cut(s) 268, 457, 1412
Bse8I GATNNNNATC 2 cut(s) 285, 339
BseBI CCWGG 2 cut(s) 11, 669
BseDI CCNNGG 1 cut(s) 10
BseGI GGATG 4 cut(s) 259, 340, 917, 1384
BseJI GATNNNNATC 2 cut(s) 285, 339
BseLI CCNNNNNNNGG 1 cut(s) 298
BseMI GCAATG 3 cut(s) 268, 457, 1412
BseMII CTCAG 2 cut(s) 849, 1251
BseNI ACTGG 7 cut(s) 75, 169, 394, 488, 608, 845, 1523
BseXI GCAGC 6 cut(s) 169, 270, 754, 868, 1076, 1153
BseYI CCCAGC 1 cut(s) 130
BsgI GTGCAG 2 cut(s) 867, 1497
BshFI GGCC 2 cut(s) 29, 74
BshNI GGYRCC 2 cut(s) 664, 1497
BshTI ACCGGT 1 cut(s) 704
BsiHKAI GWGCWC 2 cut(s) 870, 895
BsiSI CCGG 2 cut(s) 705, 851
BslFI GGGAC 3 cut(s) 163, 216, 569
BslI CCNNNNNNNGG 1 cut(s) 298
BsmFI GGGAC 3 cut(s) 163, 216, 569
BsnI GGCC 2 cut(s) 29, 74
Bsp1286I GDGCHC 2 cut(s) 870, 895
Bsp143I GATC 8 cut(s) 246, 487, 606, 959, 1019, 1207, 1240, 1285
BspACI CCGC 2 cut(s) 292, 519
BspANI GGCC 2 cut(s) 29, 74
BspCNI CTCAG 2 cut(s) 850, 1252
BspLI GGNNCC 4 cut(s) 204, 666, 924, 1499
BspMAI CTGCAG 1 cut(s) 772
BspMI ACCTGC 1 cut(s) 125
BspPI GGATC 5 cut(s) 495, 614, 1027, 1235, 1280
BspT107I GGYRCC 2 cut(s) 664, 1497
BspTI CTTAAG 1 cut(s) 526
BsrDI GCAATG 3 cut(s) 268, 457, 1412
BsrFI RCCGGY 2 cut(s) 704, 850
BsrI ACTGG 7 cut(s) 75, 169, 394, 488, 608, 845, 1523
BssAI RCCGGY 2 cut(s) 704, 850
BssECI CCNNGG 1 cut(s) 10
BssMI GATC 8 cut(s) 246, 487, 606, 959, 1019, 1207, 1240, 1285
Bst2UI CCWGG 2 cut(s) 11, 669
Bst4CI ACNGT 1 cut(s) 1450
Bst6I CTCTTC 1 cut(s) 482
BstAFI CTTAAG 1 cut(s) 526
BstAPI GCANNNNNTGC 1 cut(s) 1070
BstC8I GCNNGC 1 cut(s) 1099
BstDEI CTNAG 3 cut(s) 858, 887, 1260
BstF5I GGATG 4 cut(s) 259, 340, 917, 1384
BstKTI GATC 8 cut(s) 249, 490, 609, 962, 1022, 1210, 1243, 1288
BstMBI GATC 8 cut(s) 246, 487, 606, 959, 1019, 1207, 1240, 1285
BstMWI GCNNNNNNNGC 6 cut(s) 662, 764, 890, 1061, 1070, 1495
BstNI CCWGG 2 cut(s) 11, 669
BstSCI CCNGG 2 cut(s) 9, 667
BstSFI CTRYAG 1 cut(s) 768
BstV1I GCAGC 6 cut(s) 169, 270, 754, 868, 1076, 1153
BstV2I GAAGAC 1 cut(s) 1232
BstX2I RGATCY 3 cut(s) 487, 959, 1207
BstXI CCANNNNNNTGG 1 cut(s) 213
BstYI RGATCY 3 cut(s) 487, 959, 1207
BsuI GTATCC 2 cut(s) 1318, 1391
BsuRI GGCC 2 cut(s) 29, 74
BtsCI GGATG 4 cut(s) 259, 340, 917, 1384
BtsI GCAGTG 2 cut(s) 102, 657
BtsIMutI CAGTG 6 cut(s) 102, 481, 601, 657, 1446, 1516
BveI ACCTGC 1 cut(s) 125
Cac8I GCNNGC 1 cut(s) 1099
Cfr10I RCCGGY 2 cut(s) 704, 850
Cfr13I GGNCC 4 cut(s) 203, 360, 922, 1157
CseI GACGC 2 cut(s) 681, 1121
Csp6I GTAC 2 cut(s) 44, 720
CspAI ACCGGT 1 cut(s) 704
CviAII CATG 5 cut(s) 325, 357, 955, 1118, 1407
CviQI GTAC 2 cut(s) 44, 720
DdeI CTNAG 3 cut(s) 858, 887, 1260
DpnI GATC 8 cut(s) 248, 489, 608, 961, 1021, 1209, 1242, 1287
DpnII GATC 8 cut(s) 246, 487, 606, 959, 1019, 1207, 1240, 1285
EaeI YGGCCR 1 cut(s) 27
Eam1104I CTCTTC 1 cut(s) 482
EarI CTCTTC 1 cut(s) 482
Ecl136II GAGCTC 1 cut(s) 893
Eco24I GRGCYC 1 cut(s) 895
Eco32I GATATC 1 cut(s) 236
Eco47I GGWCC 4 cut(s) 203, 360, 922, 1157
Eco53kI GAGCTC 1 cut(s) 893
EcoICRI GAGCTC 1 cut(s) 893
EcoRI GAATTC 4 cut(s) 712, 1146, 1200, 1435
EcoRII CCWGG 2 cut(s) 9, 667
EcoRV GATATC 1 cut(s) 236
EcoT22I ATGCAT 1 cut(s) 340
EcoT38I GRGCYC 1 cut(s) 895
FaeI CATG 5 cut(s) 328, 360, 958, 1121, 1410
FalI AAGNNNNNCTT 2 cut(s) 1385, 1417
FaqI GGGAC 3 cut(s) 163, 216, 569
FatI CATG 5 cut(s) 324, 356, 954, 1117, 1406
FauNDI CATATG 1 cut(s) 212
Fnu4HI GCNGC 6 cut(s) 183, 259, 768, 882, 1065, 1167
FokI GGATG 4 cut(s) 266, 347, 924, 1391
FriOI GRGCYC 1 cut(s) 895
Fsp4HI GCNGC 6 cut(s) 183, 259, 768, 882, 1065, 1167
FspBI CTAG 6 cut(s) 41, 123, 276, 830, 896, 1418
GluI GCNGC 6 cut(s) 183, 259, 768, 882, 1065, 1167
GsaI CCCAGC 1 cut(s) 134
HaeIII GGCC 2 cut(s) 29, 74
HapII CCGG 2 cut(s) 705, 851
HgaI GACGC 2 cut(s) 681, 1121
Hin1II CATG 5 cut(s) 328, 360, 958, 1121, 1410
HincII GTYRAC 1 cut(s) 1333
HindII GTYRAC 1 cut(s) 1333
HinfI GANTC 1 cut(s) 411
HpaII CCGG 2 cut(s) 705, 851
HphI GGTGA 3 cut(s) 281, 599, 950
Hpy166II GTNNAC 2 cut(s) 400, 1333
Hpy188I TCNGA 4 cut(s) 682, 859, 1174, 1454
Hpy188III TCNNGA 5 cut(s) 239, 363, 896, 1205, 1537
Hpy8I GTNNAC 2 cut(s) 400, 1333
HpyAV CCTTC 3 cut(s) 170, 401, 1370
HpyCH4III ACNGT 1 cut(s) 1450
HpyCH4IV ACGT 3 cut(s) 1185, 1254, 1528
HpyF10VI GCNNNNNNNGC 6 cut(s) 662, 764, 890, 1061, 1070, 1495
HpyF3I CTNAG 3 cut(s) 858, 887, 1260
HpySE526I ACGT 3 cut(s) 1185, 1254, 1528
Hsp92II CATG 5 cut(s) 328, 360, 958, 1121, 1410
Kzo9I GATC 8 cut(s) 246, 487, 606, 959, 1019, 1207, 1240, 1285
LmnI GCTCC 2 cut(s) 756, 997
Lsp1109I GCAGC 6 cut(s) 169, 270, 754, 868, 1076, 1153
LweI GCATC 2 cut(s) 325, 1060
MaeI CTAG 6 cut(s) 41, 123, 276, 830, 896, 1418
MaeII ACGT 3 cut(s) 1185, 1254, 1528
MaeIII GTNAC 7 cut(s) 369, 469, 572, 772, 938, 1255, 1442
MalI GATC 8 cut(s) 248, 489, 608, 961, 1021, 1209, 1242, 1287
MboI GATC 8 cut(s) 246, 487, 606, 959, 1019, 1207, 1240, 1285
MboII GAAGA 6 cut(s) 314, 331, 469, 482, 518, 1237
MflI RGATCY 3 cut(s) 487, 959, 1207
MhlI GDGCHC 2 cut(s) 870, 895
MlsI TGGCCA 1 cut(s) 29
MluNI TGGCCA 1 cut(s) 29
MlyI GAGTC 1 cut(s) 420
MmeI TCCRAC 2 cut(s) 312, 949
MnlI CCTC 7 cut(s) 122, 485, 543, 853, 1190, 1361, 1544
Mox20I TGGCCA 1 cut(s) 29
Mph1103I ATGCAT 1 cut(s) 340
MscI TGGCCA 1 cut(s) 29
MseI TTAA 5 cut(s) 527, 615, 1140, 1295, 1392
MslI CAYNNNNRTG 2 cut(s) 192, 876
Msp20I TGGCCA 1 cut(s) 29
MspA1I CMGCKG 1 cut(s) 767
MspCI CTTAAG 1 cut(s) 526
MspI CCGG 2 cut(s) 705, 851
MspR9I CCNGG 2 cut(s) 11, 669
MvaI CCWGG 2 cut(s) 11, 669
MwoI GCNNNNNNNGC 6 cut(s) 662, 764, 890, 1061, 1070, 1495
NdeI CATATG 1 cut(s) 212
NdeII GATC 8 cut(s) 246, 487, 606, 959, 1019, 1207, 1240, 1285
NlaIII CATG 5 cut(s) 328, 360, 958, 1121, 1410
NlaIV GGNNCC 4 cut(s) 204, 666, 924, 1499
NmuCI GTSAC 1 cut(s) 938
NsiI ATGCAT 1 cut(s) 340
PinAI ACCGGT 1 cut(s) 704
PkrI GCNGC 6 cut(s) 184, 260, 769, 883, 1066, 1168
PleI GAGTC 1 cut(s) 419
PpsI GAGTC 1 cut(s) 419
Psp124BI GAGCTC 1 cut(s) 895
Psp1406I AACGTT 1 cut(s) 1528
Psp6I CCWGG 2 cut(s) 9, 667
PspFI CCCAGC 1 cut(s) 130
PspGI CCWGG 2 cut(s) 9, 667
PspN4I GGNNCC 4 cut(s) 204, 666, 924, 1499
PspPI GGNCC 4 cut(s) 203, 360, 922, 1157
PstI CTGCAG 1 cut(s) 772
PsuI RGATCY 3 cut(s) 487, 959, 1207
PvuII CAGCTG 1 cut(s) 767
RsaI GTAC 2 cut(s) 45, 721
RsaNI GTAC 2 cut(s) 44, 720
RseI CAYNNNNRTG 2 cut(s) 192, 876
SacI GAGCTC 1 cut(s) 895
SaqAI TTAA 5 cut(s) 527, 615, 1140, 1295, 1392
SatI GCNGC 6 cut(s) 183, 259, 768, 882, 1065, 1167
Sau3AI GATC 8 cut(s) 246, 487, 606, 959, 1019, 1207, 1240, 1285
Sau96I GGNCC 4 cut(s) 203, 360, 922, 1157
ScaI AGTACT 1 cut(s) 45
SchI GAGTC 1 cut(s) 420
ScrFI CCNGG 2 cut(s) 11, 669
SduI GDGCHC 2 cut(s) 870, 895
SfaNI GCATC 2 cut(s) 325, 1060
SfcI CTRYAG 1 cut(s) 768
SgrAI CRCCGGYG 1 cut(s) 704
SinI GGWCC 4 cut(s) 203, 360, 922, 1157
SmiMI CAYNNNNRTG 2 cut(s) 192, 876
SmlI CTYRAG 2 cut(s) 526, 1535
SmoI CTYRAG 2 cut(s) 526, 1535
SpeI ACTAGT 1 cut(s) 122
SsiI CCGC 2 cut(s) 292, 519
SspMI CTAG 6 cut(s) 41, 123, 276, 830, 896, 1418
SstI GAGCTC 1 cut(s) 895
StyD4I CCNGG 2 cut(s) 9, 667
TaaI ACNGT 1 cut(s) 1450
TaiI ACGT 3 cut(s) 1188, 1257, 1531
TaqI TCGA 1 cut(s) 1204
TatI WGTACW 1 cut(s) 43
Tru1I TTAA 5 cut(s) 527, 615, 1140, 1295, 1392
Tru9I TTAA 5 cut(s) 527, 615, 1140, 1295, 1392
TscAI CASTG 6 cut(s) 109, 488, 608, 657, 1453, 1523
TseFI GTSAC 1 cut(s) 938
TseI GCWGC 6 cut(s) 182, 258, 767, 881, 1064, 1166
Tsp45I GTSAC 1 cut(s) 938
TspDTI ATGAA 7 cut(s) 130, 147, 189, 297, 332, 453, 525
TspRI CASTG 6 cut(s) 109, 488, 608, 657, 1453, 1523
Vha464I CTTAAG 1 cut(s) 526
VpaK11BI GGWCC 4 cut(s) 203, 360, 922, 1157
XapI RAATTY 6 cut(s) 581, 712, 986, 1146, 1200, 1435
XbaI TCTAGA 1 cut(s) 895
XspI CTAG 6 cut(s) 41, 123, 276, 830, 896, 1418
ZrmI AGTACT 1 cut(s) 45
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.