RLG00000014749

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0009109
Physical Location & Seq
Forward (+)
57117696 .. 57123232
5537 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014749

Sequence Viewer

Length: 3327 bp
ATGGAGATCGCCCCGGCGCCGCCACAGCTTTCAGACCGGGACGTCCCAAACGACGCCGCGTCGCTAAGCACCGTCAAAACGGCGCCGTCGGAGAGCAATAACGAGGCGAACGTGTCGACGTCGTCGTCGTCGGTGGCGGCGAAGTACGACGATGAGGAGGAGGAGGAGGACGTGTGCCGGATCTGTAGGAACCCTGGCGACGCCGATAACCCGCTCAGGTATCCATGCGCGTGTAGCGGAAGCATCAAGTTCGTGCACCAGGATTGTCTCCTTCAGTGGCTCAATCACAGTAATGCTCGCCAATGCGAGGTTTGCAAGCATCCATTTTCCTTCTCTCCCGTTTATGCTGAGAATGCACCTGCAAGGCTTCCTTTTCAAGAGTTTGTAGTTGGGATGGCAATGAAAACTTGCCATGTTCTGCAATTTTTTCTGCGCCTTAGTTTTGTGCTTTCCGTTTGGCTCCTCATTATACCTTTCATTACATTTTGGATATGGCGGCTGGCTTTCGTGAGGAGTTTTGGTGAAGCTCAGAGATTATTCCTAAGTCATCTATCAACTACAGTCATTCTTACGGATTGTCTGCATGGGTTCCTACTTTCTGCTAGCATTGTGTTCATTTTTCTCGGAGCCACTTCTTTGAGGGATTACTTCAGGCATTTACGTGAGCTTGGAGGTCAAGATGTTGACAGAGAAGATGAAGCAGAAAGAAATGGCGCTCGTCCTGTAAGAAGAGTTCCTGGACAAGCTAATAGGAACTTTGTTGGGGACGCTAATGGGGAAGATGCTGCTGGAGCACAAGGGATTGTTGGAGCAGGTCAAATGATTAGAAGGAATGCGGAAAATGTGGCTGCTCGCTGGGAGATGCAGGCAGCTCGTCTTGAGGCTCATGTTGAACAGATGTTTGATGGTTTAGATGATGCTGATGGTGCAGAGGATGTACCATTTGACGAGCTTGTGGGCATGCAGGGACCTGTATTTCATTTAGTGGAAAATGCATTTACTGTACTGGCAAGCAATATGATATTCCTTGGTGTAGTAATATTTGTGCCCTTCTCATTAGGTCGGATTATACTCTATCATTTATCTTGGCTTTTCTCCACCGCTAGTGGTCCAGTTTTGTCAACAGTCATGCCACTCACTGAATCAGCCCTTTCCTTGGCTAATATGACTTTGAAGAATGCATTAACAGCTGTAACAAATTTATCAACCGATAGCGAGAAAAGTGGTATGGTTGGCCAGGTTGCAGAGATCTTGAAAGTTAACATGAGTGGACTGAACGAGGTTTCGAACAATAATGCAAGTTCACCACTTTCAGCAGACTTCCTGAAAGGGGCAGCAATTGGAACTTCAAGGCTCTCGGATGTGACAACTCTTGCTATTGGATACATGTTTATATTCTCCCTAGTTTTCTTCTACCTTGGCATTGTGGCTTTCATTCGGTACACTAGGGGCGAGCCATTGACTATGGGGAGGTTCTATGGTATTGCTTCAATGGCAGAAACAATTCCCTCTCTCTTCAGGCAGTTCCTGGCAGCAATGAGGCATTTGATGACAATGATTAAGGTCGCTTTCCTTCTGGTCATTGAACTTGGAGTGTTCCCATTAATGTGTGGATGGTGGTTGGATGTTTGTACTATAAGGATGTTTGGGAAGTCCATGTCTCACAGGGTGCAGTTCTTCTCAGCATCTCCCTTAGCTAGCTCCCTGGTCCATTGGGTTGTAGGAATTGTATACATGCTACAAATAAGCATATTCGTCAGTCTTCTTCGAGGGGTTCTGCGTAATGGAGTTCTTTATTTCCTTAGAGATCCAGCTGATCCAAACTACAATCCCTTCCGTGATCTCATTGATGATCCTGTGCACAAACATGCTCGCAGGGTCCTGTTATCCGTTGCGGTGTATGGGAGTTTAATTGTGATGCTGGTTTTTTTACCAGTTAAACTAGCTATGCGGATGGCACCTTCCATTTTTCCTCTTGACATTTCGGTATCTGACCCATTTACTGAAATTCCTGCGGACATGCTTCTATTTCAAATATGCATTCCATTTGCCATTGAGCATTTCAAATTACGGGCAACCATTAAATCCCTGCTCCGTTATTGGTTTACGGCAGTTGGCTGGGCACTTGGTTTAACAGATTTCTTACTACCCAGACCTGAGGATAACGTTGGACAGGAAAATGGAAATGCAGAGCCTGGAAGACAGGATAGACTACAGGTTCAATTGGCGCTACAGGATCAGGCTTTAGTGGCACTTCCAGGTGCTGATGAACCAAATCGAGGCCTGCTTGCATCAGGGGACACGAATGTTGCGGAGGAGTATGACACTGATGAACAATCTGATTCTGAAAGGTATAGCTTTGTGCTTCGAATTGTCCTCTTGTTGGTGGTGGCTTGGATGACTTTGCTCGTCTTCAACTCGGCCTTGATAGTTGTACCAACATCACTGGGAAGAACAATTTTCAATGTCATTCCTTTCCTCCCTATAACTCATGGCATCAAGTGCAACGATTTATATGCTTTCATCATTGGAAGCTACATAATATGGACTGCTGTAGCTGGAGTTAGATATTCTATTGAACACATTAGAACAAAAAGGGTTGCAGTTCTATTGGGCCAGATCTGGAAGTGGTGTAGCATTGTTATTAAGAGTTCTGCACTGTTGTCAATATGGATTTTTGTCATTCCAGTATTGATCGGGTTGCTCTTTGAGCTTTTGGTGATTGTGCCGATGCGGGTGCCTGTTGATGAAAGCCCAGTATTCCTGCTGTATCAGGACTGGGCTTTAGGCCTTATTTTTCTCAAGATATGGACTAGATTGGTGATGTTGGATCACATGATGCCATTGGTAGATGAAAGCTGGCGTGTCAAGTTTGAAAGGGTGAGGGAAGATGGCTTCTCCAGGCTGCAAGGTCTTTGGGTGCTCCGGGAGATTGTATTCCCAATCATAATGAAGTTGTTGACTGCCCTGTGTGTGCCTTATGTACTTGCCAGAGGGTTATTTCCTGTGCTCGGTTACCCATTAGTAGTCAACTCAGCAGTGTATCGGTTTGCCTGGATAGGATGCCTGTGCTTTAGTCTGCTGTGCTTCTGTGCCAAGAGGTTCCATGTATGGTTCACCAACCTTCACAACTCTATACGTGATGACCGATATCTAATTGGTCGTAGGCTTCATAACTTTGGAGAAGATGTTGTAGAGAAGCAAAATGAGGCAGGGGCATCCTCTGAATTGCAGGACTCTAGTTTTGAAGTCGGTGGTTTAATTCCTCATGACCGAGAGGCTGACGATGGTCTACGTCAAAGGCGTGCTATCCAACATAATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000209 GO:0001101 GO:0002020 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006508 GO:0006511 GO:0006629 GO:0006720 GO:0006725 GO:0006807 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009414 GO:0009415 GO:0009628 GO:0009698 GO:0009699 GO:0009893 GO:0009987 GO:0010025 GO:0010033 GO:0010035 GO:0010143 GO:0010166 GO:0010243 GO:0010345 GO:0010498 GO:0012505 GO:0016020 GO:0016021 GO:0016567 GO:0016740 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019787 GO:0019899 GO:0019941 GO:0030163 GO:0030176 GO:0030433 GO:0031224 GO:0031227 GO:0031624 GO:0031984 GO:0032446 GO:0032501 GO:0032502 GO:0033554 GO:0034976 GO:0036211 GO:0036503 GO:0042175 GO:0042221 GO:0042335 GO:0043085 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044390 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0048518 GO:0048856 GO:0050789 GO:0050790 GO:0050896 GO:0051341 GO:0051353 GO:0051603 GO:0051716 GO:0065007 GO:0065009 GO:0070647 GO:0070936 GO:0071704 GO:0098827 GO:0140096 GO:1900490 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901568 GO:1901570 GO:1901575 GO:1901576 GO:1901698 GO:1901700 GO:1990381 GO:2001215
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
RINGv

Protein Analysis

1109

Amino Acids

124.03

Weight (kDa)

5.83

Isoelectric Point (pI)

39.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 59 - 105 9.9e-17 RING-variant domain
MARCHF6_C PF23113 869 - 1047 7.9e-51 E3 ubiquitin-protein ligase MARCHF6-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 367
AasI GACNNNNNNGTC 2 cut(s) 41, 124
AatII GACGTC 2 cut(s) 45, 122
Acc36I ACCTGC 2 cut(s) 367, 803
AccB1I GGYRCC 4 cut(s) 16, 82, 1957, 2737
AccBSI CCGCTC 1 cut(s) 214
AccI GTMKAC 3 cut(s) 116, 1731, 3292
AccII CGCG 2 cut(s) 59, 230
AclI AACGTT 1 cut(s) 2166
AclWI GGATC 6 cut(s) 188, 1802, 1811, 1847, 2244, 2838
AcoI YGGCCR 1 cut(s) 1234
AcsI RAATTY 2 cut(s) 1198, 2007
AcuI CTGAAG 3 cut(s) 257, 634, 1501
AcyI GRCGYC 6 cut(s) 17, 42, 54, 83, 119, 201
AdeI CACNNNGTG 1 cut(s) 1669
AfaI GTAC 7 cut(s) 146, 939, 1005, 1442, 1633, 2436, 2985
AfiI CCNNNNNNNGG 6 cut(s) 307, 1156, 1330, 2279, 2383, 3011
AflIII ACRYGT 3 cut(s) 111, 171, 1386
AhdI GACNNNNNGTC 1 cut(s) 58
AjiI CACGTC 1 cut(s) 172
Alw21I GWGCWC 5 cut(s) 258, 796, 1863, 2925, 3012
Alw26I GTCTC 2 cut(s) 272, 1665
Alw44I GTGCAC 2 cut(s) 254, 1859
AlwI GGATC 6 cut(s) 188, 1802, 1811, 1847, 2244, 2838
AlwNI CAGNNNCTG 3 cut(s) 1528, 2195, 2264
AoxI GGCC 5 cut(s) 1234, 2281, 2421, 2614, 2788
ApaLI GTGCAC 2 cut(s) 254, 1859
ApeKI GCWGC 6 cut(s) 785, 848, 869, 1334, 1532, 2905
ApoI RAATTY 2 cut(s) 1198, 2007
ArsI GACNNNNNNTTYG 2 cut(s) 3227, 3259
AseI ATTAAT 1 cut(s) 1604
Asp700I GAANNNNTTC 1 cut(s) 1503
AspLEI GCGC 6 cut(s) 19, 85, 230, 435, 716, 2230
AspS9I GGNCC 5 cut(s) 968, 1109, 1708, 1879, 2614
AsuC2I CCSGG 3 cut(s) 14, 38, 2927
AsuHPI GGTGA 6 cut(s) 533, 1296, 2731, 2833, 2893, 3109
AsuII TTCGAA 2 cut(s) 1286, 2368
AsuNHI GCTAGC 2 cut(s) 602, 1697
AvaII GGWCC 4 cut(s) 968, 1109, 1708, 1879
AxyI CCTNAGG 1 cut(s) 2157
BaeGI GKGCMC 4 cut(s) 258, 1050, 1863, 2125
BalI TGGCCA 1 cut(s) 1236
BanI GGYRCC 4 cut(s) 16, 82, 1957, 2737
BbsI GAAGAC 3 cut(s) 1754, 2206, 2404
Bbv12I GWGCWC 5 cut(s) 258, 796, 1863, 2925, 3012
BbvI GCAGC 6 cut(s) 772, 835, 881, 1346, 1544, 2892
BccI CCATC 7 cut(s) 388, 899, 917, 1608, 1948, 2885, 3281
BceAI ACGGC 3 cut(s) 70, 96, 2124
BcgI CGANNNNNNTGC 8 cut(s) 232, 266, 854, 888, 2498, 2532, 2719, 2753
BciVI GTATCC 2 cut(s) 231, 1376
BcnI CCSGG 3 cut(s) 14, 38, 2927
BcoDI GTCTC 2 cut(s) 272, 1665
BfaI CTAG 8 cut(s) 603, 1104, 1403, 1446, 1698, 1943, 2814, 3240
BfmI CTRYAG 5 cut(s) 184, 558, 2213, 2231, 2553
BfoI RGCGCY 4 cut(s) 20, 86, 717, 2231
BfuAI ACCTGC 2 cut(s) 367, 803
BfuI GTATCC 2 cut(s) 231, 1376
BglII AGATCT 2 cut(s) 1248, 2619
BlpI GCTNAGC 1 cut(s) 65
Bme18I GGWCC 4 cut(s) 968, 1109, 1708, 1879
BmeRI GACNNNNNGTC 1 cut(s) 58
BmgBI CACGTC 1 cut(s) 172
BmgT120I GGNCC 5 cut(s) 968, 1109, 1708, 1879, 2614
BmrI ACTGGG 3 cut(s) 2456, 2750, 2788
BmtI GCTAGC 2 cut(s) 606, 1701
BmuI ACTGGG 3 cut(s) 2456, 2750, 2788
BoxI GACNNNNGTC 1 cut(s) 3288
BpiI GAAGAC 3 cut(s) 1754, 2206, 2404
BpmI CTGGAG 3 cut(s) 810, 2580, 2884
Bpu10I CCTNAGC 2 cut(s) 215, 1693
Bpu1102I GCTNAGC 1 cut(s) 65
Bpu14I TTCGAA 2 cut(s) 1286, 2368
BpuEI CTTGAG 2 cut(s) 899, 2786
BpuMI CCSGG 3 cut(s) 14, 38, 2927
BsaAI YACGTR 2 cut(s) 662, 3140
BsaHI GRCGYC 6 cut(s) 17, 42, 54, 83, 119, 201
BsaJI CCNNGG 6 cut(s) 12, 193, 1027, 1155, 1417, 1704
BsaXI ACNNNNNCTCC 6 cut(s) 158, 188, 505, 535, 3174, 3204
Bsc4I CCNNNNNNNGG 6 cut(s) 307, 1156, 1330, 2279, 2383, 3011
Bse1I ACTGG 7 cut(s) 1011, 1112, 1934, 2451, 2687, 2756, 2783
Bse21I CCTNAGG 1 cut(s) 2157
Bse3DI GCAATG 2 cut(s) 405, 1542
BseDI CCNNGG 6 cut(s) 12, 193, 1027, 1155, 1417, 1704
BseLI CCNNNNNNNGG 6 cut(s) 307, 1156, 1330, 2279, 2383, 3011
BseMI GCAATG 2 cut(s) 405, 1542
BseMII CTCAG 6 cut(s) 229, 339, 542, 1695, 2148, 3048
BseNI ACTGG 7 cut(s) 1011, 1112, 1934, 2451, 2687, 2756, 2783
BseRI GAGGAG 7 cut(s) 170, 173, 176, 179, 452, 526, 2330
BseSI GKGCMC 4 cut(s) 258, 1050, 1863, 2125
BseXI GCAGC 6 cut(s) 772, 835, 881, 1346, 1544, 2892
BseYI CCCAGC 2 cut(s) 855, 2118
BsgI GTGCAG 3 cut(s) 948, 1691, 2640
Bsh1236I CGCG 2 cut(s) 59, 230
BshFI GGCC 5 cut(s) 1236, 2283, 2423, 2616, 2790
BshNI GGYRCC 4 cut(s) 16, 82, 1957, 2737
BsiHKAI GWGCWC 5 cut(s) 258, 796, 1863, 2925, 3012
BsiSI CCGG 4 cut(s) 14, 37, 178, 2926
BslFI GGGAC 5 cut(s) 29, 53, 779, 981, 2312
BslI CCNNNNNNNGG 6 cut(s) 307, 1156, 1330, 2279, 2383, 3011
BsmAI GTCTC 2 cut(s) 272, 1665
BsmFI GGGAC 5 cut(s) 29, 53, 779, 981, 2312
BsmI GAATGC 4 cut(s) 358, 838, 1183, 2040
BsnI GGCC 5 cut(s) 1236, 2283, 2423, 2616, 2790
Bsp119I TTCGAA 2 cut(s) 1286, 2368
Bsp1286I GDGCHC 7 cut(s) 258, 796, 1050, 1863, 2125, 2925, 3012
Bsp1720I GCTNAGC 1 cut(s) 65
BspANI GGCC 5 cut(s) 1236, 2283, 2423, 2616, 2790
BspCNI CTCAG 6 cut(s) 228, 340, 541, 1694, 2149, 3047
BspFNI CGCG 2 cut(s) 59, 230
BspHI TCATGA 1 cut(s) 3268
BspMI ACCTGC 2 cut(s) 367, 803
BspOI GCTAGC 2 cut(s) 606, 1701
BspPI GGATC 6 cut(s) 188, 1802, 1811, 1847, 2244, 2838
BspT104I TTCGAA 2 cut(s) 1286, 2368
BspT107I GGYRCC 4 cut(s) 16, 82, 1957, 2737
BsrBI CCGCTC 1 cut(s) 214
BsrDI GCAATG 2 cut(s) 405, 1542
BsrI ACTGG 7 cut(s) 1011, 1112, 1934, 2451, 2687, 2756, 2783
BssECI CCNNGG 6 cut(s) 12, 193, 1027, 1155, 1417, 1704
BssNAI GTATAC 1 cut(s) 1732
BssNI GRCGYC 6 cut(s) 17, 42, 54, 83, 119, 201
BssT1I CCWWGG 3 cut(s) 1027, 1155, 1417
Bst1107I GTATAC 1 cut(s) 1732
Bst4CI ACNGT 6 cut(s) 73, 290, 562, 1003, 1126, 2661
Bst6I CTCTTC 2 cut(s) 724, 1520
BstACI GRCGYC 6 cut(s) 17, 42, 54, 83, 119, 201
BstAPI GCANNNNNTGC 1 cut(s) 2502
BstBAI YACGTR 2 cut(s) 662, 3140
BstBI TTCGAA 2 cut(s) 1286, 2368
BstEII GGTNACC 1 cut(s) 3014
BstFNI CGCG 2 cut(s) 59, 230
BstH2I RGCGCY 4 cut(s) 20, 86, 717, 2231
BstHHI GCGC 6 cut(s) 19, 85, 230, 435, 716, 2230
BstMAI GTCTC 2 cut(s) 272, 1665
BstNSI RCATGY 5 cut(s) 964, 1390, 1738, 1871, 2023
BstPAI GACNNNNGTC 1 cut(s) 3288
BstPI GGTNACC 1 cut(s) 3014
BstSFI CTRYAG 5 cut(s) 184, 558, 2213, 2231, 2553
BstSLI GKGCMC 4 cut(s) 258, 1050, 1863, 2125
BstUI CGCG 2 cut(s) 59, 230
BstV1I GCAGC 6 cut(s) 772, 835, 881, 1346, 1544, 2892
BstV2I GAAGAC 3 cut(s) 1754, 2206, 2404
BstX2I RGATCY 4 cut(s) 180, 1248, 1807, 2619
BstYI RGATCY 4 cut(s) 180, 1248, 1807, 2619
BstZ17I GTATAC 1 cut(s) 1732
Bsu36I CCTNAGG 1 cut(s) 2157
BsuI GTATCC 2 cut(s) 231, 1376
BsuRI GGCC 5 cut(s) 1236, 2283, 2423, 2616, 2790
BtrI CACGTC 1 cut(s) 172
BtsI GCAGTG 1 cut(s) 3045
BtsIMutI CAGTG 6 cut(s) 281, 1137, 2325, 2444, 2657, 3045
BveI ACCTGC 2 cut(s) 367, 803
CaiI CAGNNNCTG 3 cut(s) 1528, 2195, 2264
CciI TCATGA 1 cut(s) 3268
CfoI GCGC 6 cut(s) 19, 85, 230, 435, 716, 2230
Cfr13I GGNCC 5 cut(s) 968, 1109, 1708, 1879, 2614
CseI GACGC 4 cut(s) 48, 62, 209, 776
Csp6I GTAC 7 cut(s) 145, 938, 1004, 1441, 1632, 2435, 2984
CviQI GTAC 7 cut(s) 145, 938, 1004, 1441, 1632, 2435, 2984
DinI GGCGCC 2 cut(s) 18, 84
DraIII CACNNNGTG 1 cut(s) 1669
DrdI GACNNNNNNGTC 2 cut(s) 41, 124
DriI GACNNNNNGTC 1 cut(s) 58
DseDI GACNNNNNNGTC 2 cut(s) 41, 124
EaeI YGGCCR 1 cut(s) 1234
Eam1104I CTCTTC 2 cut(s) 724, 1520
Eam1105I GACNNNNNGTC 1 cut(s) 58
EarI CTCTTC 2 cut(s) 724, 1520
Eco130I CCWWGG 3 cut(s) 1027, 1155, 1417
Eco147I AGGCCT 2 cut(s) 2283, 2790
Eco32I GATATC 1 cut(s) 3152
Eco47I GGWCC 4 cut(s) 968, 1109, 1708, 1879
Eco57I CTGAAG 3 cut(s) 257, 634, 1501
Eco81I CCTNAGG 1 cut(s) 2157
Eco91I GGTNACC 1 cut(s) 3014
EcoO109I RGGNCCY 2 cut(s) 968, 1879
EcoO65I GGTNACC 1 cut(s) 3014
EcoRV GATATC 1 cut(s) 3152
EcoT14I CCWWGG 3 cut(s) 1027, 1155, 1417
EcoT22I ATGCAT 3 cut(s) 997, 1183, 2042
EgeI GGCGCC 2 cut(s) 18, 84
EheI GGCGCC 2 cut(s) 18, 84
ErhI CCWWGG 3 cut(s) 1027, 1155, 1417
FalI AAGNNNNNCTT 2 cut(s) 355, 387
FaqI GGGAC 5 cut(s) 29, 53, 779, 981, 2312
FauI CCCGC 2 cut(s) 219, 2727
FblI GTMKAC 3 cut(s) 116, 1731, 3292
FspBI CTAG 8 cut(s) 603, 1104, 1403, 1446, 1698, 1943, 2814, 3240
GlaI GCGC 6 cut(s) 18, 84, 229, 434, 715, 2229
GsaI CCCAGC 2 cut(s) 859, 2122
GsuI CTGGAG 3 cut(s) 810, 2580, 2884
HaeII RGCGCY 4 cut(s) 20, 86, 717, 2231
HaeIII GGCC 5 cut(s) 1236, 2283, 2423, 2616, 2790
HapII CCGG 4 cut(s) 14, 37, 178, 2926
HgaI GACGC 4 cut(s) 48, 62, 209, 776
HhaI GCGC 6 cut(s) 19, 85, 230, 435, 716, 2230
Hin1I GRCGYC 6 cut(s) 17, 42, 54, 83, 119, 201
Hin6I GCGC 6 cut(s) 17, 83, 228, 433, 714, 2228
HinP1I GCGC 6 cut(s) 17, 83, 228, 433, 714, 2228
HincII GTYRAC 6 cut(s) 117, 685, 1122, 1261, 2961, 3031
HindII GTYRAC 6 cut(s) 117, 685, 1122, 1261, 2961, 3031
HinfI GANTC 3 cut(s) 1142, 2342, 3236
HpaI GTTAAC 1 cut(s) 1261
HpaII CCGG 4 cut(s) 14, 37, 178, 2926
HphI GGTGA 6 cut(s) 533, 1296, 2731, 2833, 2893, 3109
HpyAV CCTTC 8 cut(s) 281, 340, 822, 1060, 1583, 1843, 1971, 3134
HpyCH4III ACNGT 6 cut(s) 73, 290, 562, 1003, 1126, 2661
HpyCH4IV ACGT 8 cut(s) 42, 111, 119, 171, 661, 2166, 3139, 3295
HpySE526I ACGT 8 cut(s) 42, 111, 119, 171, 661, 2166, 3139, 3295
Hsp92I GRCGYC 6 cut(s) 17, 42, 54, 83, 119, 201
HspAI GCGC 6 cut(s) 17, 83, 228, 433, 714, 2228
KasI GGCGCC 2 cut(s) 16, 82
KspAI GTTAAC 1 cut(s) 1261
LmnI GCTCC 7 cut(s) 465, 626, 791, 809, 1706, 2097, 2928
Lsp1109I GCAGC 6 cut(s) 772, 835, 881, 1346, 1544, 2892
MaeI CTAG 8 cut(s) 603, 1104, 1403, 1446, 1698, 1943, 2814, 3240
MaeII ACGT 8 cut(s) 42, 111, 119, 171, 661, 2166, 3139, 3295
MaeIII GTNAC 3 cut(s) 1192, 1363, 3014
MbiI CCGCTC 1 cut(s) 214
MfeI CAATTG 2 cut(s) 1338, 2222
MflI RGATCY 4 cut(s) 180, 1248, 1807, 2619
MhlI GDGCHC 7 cut(s) 258, 796, 1050, 1863, 2125, 2925, 3012
MlsI TGGCCA 1 cut(s) 1236
MluNI TGGCCA 1 cut(s) 1236
Mly113I GGCGCC 2 cut(s) 17, 83
MlyI GAGTC 1 cut(s) 3230
MmeI TCCRAC 6 cut(s) 69, 787, 1043, 1602, 2149, 2808
Mox20I TGGCCA 1 cut(s) 1236
Mph1103I ATGCAT 3 cut(s) 997, 1183, 2042
MroXI GAANNNNTTC 1 cut(s) 1503
MscI TGGCCA 1 cut(s) 1236
MslI CAYNNNNRTG 5 cut(s) 229, 291, 660, 1606, 1866
Msp20I TGGCCA 1 cut(s) 1236
MspA1I CMGCKG 2 cut(s) 1190, 1814
MspI CCGG 4 cut(s) 14, 37, 178, 2926
MunI CAATTG 2 cut(s) 1338, 2222
Mva1269I GAATGC 4 cut(s) 358, 838, 1183, 2040
MvnI CGCG 2 cut(s) 59, 230
NarI GGCGCC 2 cut(s) 17, 83
NciI CCSGG 3 cut(s) 14, 38, 2927
NheI GCTAGC 2 cut(s) 602, 1697
NmeAIII GCCGAG 1 cut(s) 2399
NmuCI GTSAC 1 cut(s) 1363
NsiI ATGCAT 3 cut(s) 997, 1183, 2042
NspI RCATGY 5 cut(s) 964, 1390, 1738, 1871, 2023
NspV TTCGAA 2 cut(s) 1286, 2368
PaeI GCATGC 1 cut(s) 964
PagI TCATGA 1 cut(s) 3268
PaqCI CACCTGC 1 cut(s) 367
PceI AGGCCT 2 cut(s) 2283, 2790
PciI ACATGT 1 cut(s) 1386
PcsI WCGNNNNNNNCGW 7 cut(s) 48, 108, 122, 125, 137, 3145, 3301
PctI GAATGC 4 cut(s) 358, 838, 1183, 2040
PdmI GAANNNNTTC 1 cut(s) 1503
PfeI GAWTC 2 cut(s) 1142, 2342
PflFI GACNNNGTC 1 cut(s) 121
PfoI TCCNGGA 2 cut(s) 736, 2925
PleI GAGTC 1 cut(s) 3230
PluTI GGCGCC 2 cut(s) 20, 86
PpsI GAGTC 1 cut(s) 3230
Ppu21I YACGTR 2 cut(s) 662, 3140
PpuMI RGGWCCY 2 cut(s) 968, 1879
PscI ACATGT 1 cut(s) 1386
PshAI GACNNNNGTC 1 cut(s) 3288
PshBI ATTAAT 1 cut(s) 1604
Psp1406I AACGTT 1 cut(s) 2166
Psp5II RGGWCCY 2 cut(s) 968, 1879
PspEI GGTNACC 1 cut(s) 3014
PspFI CCCAGC 2 cut(s) 855, 2118
PspPI GGNCC 5 cut(s) 968, 1109, 1708, 1879, 2614
PspPPI RGGWCCY 2 cut(s) 968, 1879
PsrI GAACNNNNNNTAC 2 cut(s) 717, 749
PstNI CAGNNNCTG 3 cut(s) 1528, 2195, 2264
PsuI RGATCY 4 cut(s) 180, 1248, 1807, 2619
PsyI GACNNNGTC 1 cut(s) 121
PvuII CAGCTG 2 cut(s) 1190, 1814
RsaI GTAC 7 cut(s) 146, 939, 1005, 1442, 1633, 2436, 2985
RsaNI GTAC 7 cut(s) 145, 938, 1004, 1441, 1632, 2435, 2984
RseI CAYNNNNRTG 5 cut(s) 229, 291, 660, 1606, 1866
SalI GTCGAC 1 cut(s) 115
Sau96I GGNCC 5 cut(s) 968, 1109, 1708, 1879, 2614
SchI GAGTC 1 cut(s) 3230
SduI GDGCHC 7 cut(s) 258, 796, 1050, 1863, 2125, 2925, 3012
SfcI CTRYAG 5 cut(s) 184, 558, 2213, 2231, 2553
SfoI GGCGCC 2 cut(s) 18, 84
SfuI TTCGAA 2 cut(s) 1286, 2368
SinI GGWCC 4 cut(s) 968, 1109, 1708, 1879
SmiMI CAYNNNNRTG 5 cut(s) 229, 291, 660, 1606, 1866
SmlI CTYRAG 2 cut(s) 878, 2801
SmoI CTYRAG 2 cut(s) 878, 2801
SphI GCATGC 1 cut(s) 964
SseBI AGGCCT 2 cut(s) 2283, 2790
SspDI GGCGCC 2 cut(s) 16, 82
SspI AATATT 1 cut(s) 1041
SspMI CTAG 8 cut(s) 603, 1104, 1403, 1446, 1698, 1943, 2814, 3240
StuI AGGCCT 2 cut(s) 2283, 2790
StyI CCWWGG 3 cut(s) 1027, 1155, 1417
TaaI ACNGT 6 cut(s) 73, 290, 562, 1003, 1126, 2661
TaiI ACGT 8 cut(s) 45, 114, 122, 174, 664, 2169, 3142, 3298
TaqI TCGA 5 cut(s) 116, 1286, 1768, 2278, 2368
TaqII GACCGA 2 cut(s) 3162, 3288
TatI WGTACW 3 cut(s) 1003, 1631, 2983
TauI GCSGC 4 cut(s) 22, 59, 140, 499
TfiI GAWTC 2 cut(s) 1142, 2342
TscAI CASTG 6 cut(s) 281, 1144, 2332, 2451, 2664, 3045
TseFI GTSAC 1 cut(s) 1363
TseI GCWGC 6 cut(s) 785, 848, 869, 1334, 1532, 2905
Tsp45I GTSAC 1 cut(s) 1363
TspGWI ACGGA 5 cut(s) 442, 587, 1826, 1879, 2084
TspRI CASTG 6 cut(s) 281, 1144, 2332, 2451, 2664, 3045
Tth111I GACNNNGTC 1 cut(s) 121
VneI GTGCAC 2 cut(s) 254, 1859
VpaK11BI GGWCC 4 cut(s) 968, 1109, 1708, 1879
VspI ATTAAT 1 cut(s) 1604
XapI RAATTY 2 cut(s) 1198, 2007
XceI RCATGY 5 cut(s) 964, 1390, 1738, 1871, 2023
XmiI GTMKAC 3 cut(s) 116, 1731, 3292
XmnI GAANNNNTTC 1 cut(s) 1503
XspI CTAG 8 cut(s) 603, 1104, 1403, 1446, 1698, 1943, 2814, 3240
ZraI GACGTC 2 cut(s) 43, 120
Zsp2I ATGCAT 3 cut(s) 997, 1183, 2042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.