Rmu_sc0003012.1_g000002

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003012.1
OG0009109
Physical Location & Seq
Forward (+)
3596 .. 9268
5673 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003012.1_g000002.1.cds

Sequence Viewer

Length: 2922 bp
atggagatcgccccggcgccgccacagctttcagaccgggacgtcccaaacgacgccgcgtcgctaagcaccgtcaaaacggcgccgtcggagagcaacaacgaggcgaacgtgtcgacgtcgtcgtcgtcggtggcggggaagtacgacgatgaggaagaggaggaggacgtgtgccggatctgtaggaaccctggcgacgccgataacccgctcagcattgtgttcatttttctcggagccacttctctgagggattacttcaggcatttacgtgagcttggaggtcaagatgttgacagagaagatgaagcagaaagaaatggcgctcgtcctgtaagaagagttcctggacaagctaataggaactttgttggggatgctaacggggaagatgctgctggagcacaagggattgctggagcaggtcaaatgattagaaggaatgcagaaaatgtggctgctcgctgggagatgcaggcagctcgtcttgaggctcatgttgaacagatgtttgatggtctagacgatgccgatggtgcagaggatgtaccatttgacgagcttgtgggcatgcagggacctgtatttcatttagtggaaaatgcatttactgtactggcaagcaatatgatattccttggtgtagtaatatttgtgcccttctcgttaggtcggattatactctatcatttatcttggcttttctccaccgctagtggtccagttttgtcaacagtcatgccactcactgaatcagccctttccttggctaatatgactttgaagaatgcattaacagctgtaacaaatttatcaaccgatagcgagaaaagtggtatggttggccaggttgcagagatcttgaaagttaacatgagtggactgaatgaggtttcaaacaataatgcaagttcaacactttcagcagacttcctgaaaggggcaacaattggaacttcaaggctctctgatgtgacaactcttgctattggatacatgtttatattctccctagttttcttctaccttggtattgtggctttcattcggtatactaggggtgagccactgactatggggaggttttatggtattgcttcaatggcagaaacaattccatctctcttcaggcagttcctggcagcaatgaggcatttgatgacaatgattaaggtcgctttccttctggtcattgaacttggagtgttcccattaatgtgtggatggtggttggatgtttgtactataaggatgtttgggaagtccatgtctcacagggtgcagttcttctcagcatctcccttagctagctccctggtccattgggttgtaggaatcgtatacatgctacaaataagcatattcgtcagtcttcttcgaggggttctgcgtaatggagttctttatttccttagagatccagctgatccaaactacaatcccttccgtgatctcattgatgatcctgtgcacaagcatgctcgcagggtcctgttatccgttgcggtgtatgggagtttaattgtgatgctggtgtttttaccagttaaactagctatgcggatggcaccttccatttttcctcttgacatttcggtatctgacccatttactgaaattcctgcggacatgcttctatttcaaatatgcattccatttgccattgagcatttcaaattacgggcaaccattaaatccctgctccgttattggattacggcagtttgctgggcacttggtttaacagatttcttactacccatacctgaggataacgttggacaggaaaatggaaatgcagagcctggaagacaattggggctacaggatcaggctttagtggcacttccaggtgctgatgaaccaaatcgaggccttcttgcatcaggggacgcgaatgttgcagaggagtatgacactgatgaacaatctgattctgaaaggtatagctttgtgcttcgaattgtcctcttgttggtggtggcttggatgactttactcgtcttcaactcggccttgatagttgtaccaacatcactgggaagaacaattttcaatgtcattcctttcctccctataactcatggcatcaagtgcaacgatttatatgctttcatcattggaagctacataatatggactgctgtagctggagttagatattctattgaacacattagaacaaaaagggttgcagttctattgggccagatctggaagtggtgtagcattgtcattaagagttctgcactgttgtcaatatggatttttgtcattccagtattgatcgggttgctctttgagcttttggtgattgtgccgatgcgggtgcctgttgatgaaagcccagtattcctgctgtatcaggactgggctttaggccttatttttctcaagatatggacaagattggtgatgttggatcacatgatgccattggtagatgaaagctggcgcgtaaagtttgaaagggtgagggaagatggcttctccaggctgcaaggtctttgggtgctccgggagattgtattcccaatcataatgaagttgttgactgccctgtgtgtgccttatgtacttgcaagagggttatttcctgtgctcggttacccattagtagtcaactcagcagtgtatcggtttgcctggataggatgcctgtgctttagtctgctgtgcttctgtgccaagaggttccatgtatggttcaccaaccttcacaactctatacgtgatgaccgatatctaattggtcgtaggcttcataactttggagaagatgttgtagagaagcaaaatgaggcagggacatcctctgaattgcaggactctagttttgaagccagtggtttaattcctcatgaccgagaggctgacgatggtctccgtcaaaggcgtgctatccaacataatgcttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000209 GO:0001101 GO:0002020 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006508 GO:0006511 GO:0006629 GO:0006720 GO:0006725 GO:0006807 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009414 GO:0009415 GO:0009628 GO:0009698 GO:0009699 GO:0009893 GO:0009987 GO:0010025 GO:0010033 GO:0010035 GO:0010143 GO:0010166 GO:0010243 GO:0010345 GO:0010498 GO:0012505 GO:0016020 GO:0016021 GO:0016567 GO:0016740 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019787 GO:0019899 GO:0019941 GO:0030163 GO:0030176 GO:0030433 GO:0031224 GO:0031227 GO:0031624 GO:0031984 GO:0032446 GO:0032501 GO:0032502 GO:0033554 GO:0034976 GO:0036211 GO:0036503 GO:0042175 GO:0042221 GO:0042335 GO:0043085 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044390 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0048518 GO:0048856 GO:0050789 GO:0050790 GO:0050896 GO:0051341 GO:0051353 GO:0051603 GO:0051716 GO:0065007 GO:0065009 GO:0070647 GO:0070936 GO:0071704 GO:0098827 GO:0140096 GO:1900490 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901568 GO:1901570 GO:1901575 GO:1901576 GO:1901698 GO:1901700 GO:1990381 GO:2001215
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
RINGv

Protein Analysis

973

Amino Acids

108.35

Weight (kDa)

5.38

Isoelectric Point (pI)

38.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 41, 124
AatII GACGTC 2 cut(s) 45, 122
Acc36I ACCTGC 1 cut(s) 416
AccB1I GGYRCC 4 cut(s) 16, 82, 1570, 2332
AccBSI CCGCTC 1 cut(s) 214
AccI GTMKAC 3 cut(s) 116, 1055, 1344
AccII CGCG 3 cut(s) 59, 1898, 2460
AciI CCGC 9 cut(s) 20, 57, 137, 212, 714, 1508, 1564, 1628, 2329
AclI AACGTT 1 cut(s) 1779
AclWI GGATC 6 cut(s) 188, 1415, 1424, 1460, 1839, 2433
AcoI YGGCCR 1 cut(s) 847
AcsI RAATTY 2 cut(s) 811, 1620
AcuI CTGAAG 2 cut(s) 247, 1114
AcyI GRCGYC 6 cut(s) 17, 42, 54, 83, 119, 201
AdeI CACNNNGTG 1 cut(s) 1282
AfaI GTAC 6 cut(s) 146, 552, 618, 1246, 2031, 2580
AfiI CCNNNNNNNGG 5 cut(s) 769, 943, 1874, 1978, 2606
AflIII ACRYGT 3 cut(s) 111, 171, 999
AhdI GACNNNNNGTC 1 cut(s) 58
AjiI CACGTC 1 cut(s) 172
AjnI CCWGG 9 cut(s) 193, 349, 849, 1140, 1317, 1807, 1852, 2495, 2648
Alw21I GWGCWC 4 cut(s) 409, 1476, 2520, 2607
Alw26I GTCTC 2 cut(s) 1278, 2891
Alw44I GTGCAC 1 cut(s) 1472
AlwI GGATC 6 cut(s) 188, 1415, 1424, 1460, 1839, 2433
AlwNI CAGNNNCTG 3 cut(s) 1141, 1808, 1859
AoxI GGCC 5 cut(s) 847, 1876, 2016, 2209, 2383
ApaLI GTGCAC 1 cut(s) 1472
ApeKI GCWGC 5 cut(s) 398, 461, 482, 1145, 2500
ApoI RAATTY 2 cut(s) 811, 1620
ArsI GACNNNNNNTTYG 2 cut(s) 2822, 2854
AseI ATTAAT 1 cut(s) 1217
Asp700I GAANNNNTTC 1 cut(s) 1116
AspLEI GCGC 4 cut(s) 19, 85, 329, 2460
AsuC2I CCSGG 3 cut(s) 14, 38, 2522
AsuHPI GGTGA 5 cut(s) 1076, 2326, 2428, 2488, 2704
AsuII TTCGAA 1 cut(s) 1963
AsuNHI GCTAGC 1 cut(s) 1310
AvaII GGWCC 4 cut(s) 581, 722, 1321, 1492
AxyI CCTNAGG 1 cut(s) 1770
BaeGI GKGCMC 3 cut(s) 663, 1476, 1738
BalI TGGCCA 1 cut(s) 849
BanI GGYRCC 4 cut(s) 16, 82, 1570, 2332
BbsI GAAGAC 3 cut(s) 1367, 1819, 1999
Bbv12I GWGCWC 4 cut(s) 409, 1476, 2520, 2607
BbvI GCAGC 5 cut(s) 385, 448, 494, 1157, 2487
BccI CCATC 7 cut(s) 512, 530, 1129, 1221, 1561, 2480, 2876
BceAI ACGGC 3 cut(s) 70, 96, 1737
BcgI CGANNNNNNTGC 6 cut(s) 467, 501, 2093, 2127, 2314, 2348
BciT130I CCWGG 9 cut(s) 195, 351, 851, 1142, 1319, 1809, 1854, 2497, 2650
BciVI GTATCC 1 cut(s) 989
BcnI CCSGG 3 cut(s) 14, 38, 2522
BcoDI GTCTC 2 cut(s) 1278, 2891
BfaI CTAG 7 cut(s) 524, 717, 1016, 1059, 1311, 1556, 2835
BfmI CTRYAG 3 cut(s) 184, 1826, 2148
BfoI RGCGCY 3 cut(s) 20, 86, 330
BfuAI ACCTGC 1 cut(s) 416
BfuI GTATCC 1 cut(s) 989
BglII AGATCT 2 cut(s) 861, 2214
BisI GCNGC 7 cut(s) 20, 57, 399, 462, 483, 1146, 2501
BlpI GCTNAGC 2 cut(s) 65, 215
BlsI GCNGC 7 cut(s) 21, 58, 400, 463, 484, 1147, 2502
Bme18I GGWCC 4 cut(s) 581, 722, 1321, 1492
BmeRI GACNNNNNGTC 1 cut(s) 58
BmgBI CACGTC 1 cut(s) 172
BmgT120I GGNCC 5 cut(s) 581, 722, 1321, 1492, 2209
BmiI GGNNCC 9 cut(s) 18, 84, 191, 241, 582, 1493, 1572, 2334, 2699
BmrI ACTGGG 3 cut(s) 2051, 2345, 2383
BmtI GCTAGC 1 cut(s) 1314
BmuI ACTGGG 3 cut(s) 2051, 2345, 2383
BoxI GACNNNNGTC 1 cut(s) 2883
BpiI GAAGAC 3 cut(s) 1367, 1819, 1999
BpmI CTGGAG 4 cut(s) 423, 441, 2175, 2479
Bpu10I CCTNAGC 1 cut(s) 1306
Bpu1102I GCTNAGC 2 cut(s) 65, 215
Bpu14I TTCGAA 1 cut(s) 1963
BpuEI CTTGAG 2 cut(s) 512, 2381
BpuMI CCSGG 3 cut(s) 14, 38, 2522
BsaAI YACGTR 2 cut(s) 275, 2735
BsaHI GRCGYC 6 cut(s) 17, 42, 54, 83, 119, 201
BsaI GGTCTC 1 cut(s) 2891
BsaJI CCNNGG 6 cut(s) 12, 193, 640, 768, 1030, 1317
BsaXI ACNNNNNCTCC 4 cut(s) 158, 188, 2769, 2799
Bsc4I CCNNNNNNNGG 5 cut(s) 769, 943, 1874, 1978, 2606
Bse1I ACTGG 8 cut(s) 624, 725, 1547, 2046, 2282, 2351, 2378, 2847
Bse21I CCTNAGG 1 cut(s) 1770
Bse3DI GCAATG 1 cut(s) 1155
BseBI CCWGG 9 cut(s) 195, 351, 851, 1142, 1319, 1809, 1854, 2497, 2650
BseDI CCNNGG 6 cut(s) 12, 193, 640, 768, 1030, 1317
BseGI GGATG 9 cut(s) 385, 553, 1232, 1243, 1260, 1572, 1998, 2663, 2813
BseLI CCNNNNNNNGG 5 cut(s) 769, 943, 1874, 1978, 2606
BseMI GCAATG 1 cut(s) 1155
BseMII CTCAG 5 cut(s) 229, 242, 1308, 1761, 2643
BseNI ACTGG 8 cut(s) 624, 725, 1547, 2046, 2282, 2351, 2378, 2847
BseRI GAGGAG 3 cut(s) 176, 179, 1925
BseSI GKGCMC 3 cut(s) 663, 1476, 1738
BseXI GCAGC 5 cut(s) 385, 448, 494, 1157, 2487
BseYI CCCAGC 2 cut(s) 468, 1731
BsgI GTGCAG 3 cut(s) 561, 1304, 2235
Bsh1236I CGCG 3 cut(s) 59, 1898, 2460
BshFI GGCC 5 cut(s) 849, 1878, 2018, 2211, 2385
BshNI GGYRCC 4 cut(s) 16, 82, 1570, 2332
BsiHKAI GWGCWC 4 cut(s) 409, 1476, 2520, 2607
BsiSI CCGG 4 cut(s) 14, 37, 178, 2521
BslFI GGGAC 5 cut(s) 29, 53, 594, 1907, 2824
BslI CCNNNNNNNGG 5 cut(s) 769, 943, 1874, 1978, 2606
BsmAI GTCTC 2 cut(s) 1278, 2891
BsmFI GGGAC 5 cut(s) 29, 53, 594, 1907, 2824
BsmI GAATGC 3 cut(s) 451, 796, 1653
BsnI GGCC 5 cut(s) 849, 1878, 2018, 2211, 2385
Bso31I GGTCTC 1 cut(s) 2891
Bsp119I TTCGAA 1 cut(s) 1963
Bsp1286I GDGCHC 6 cut(s) 409, 663, 1476, 1738, 2520, 2607
Bsp1720I GCTNAGC 2 cut(s) 65, 215
BspACI CCGC 9 cut(s) 20, 57, 137, 212, 714, 1508, 1564, 1628, 2329
BspANI GGCC 5 cut(s) 849, 1878, 2018, 2211, 2385
BspCNI CTCAG 5 cut(s) 228, 243, 1307, 1762, 2642
BspFNI CGCG 3 cut(s) 59, 1898, 2460
BspHI TCATGA 1 cut(s) 2863
BspLI GGNNCC 9 cut(s) 18, 84, 191, 241, 582, 1493, 1572, 2334, 2699
BspMI ACCTGC 1 cut(s) 416
BspOI GCTAGC 1 cut(s) 1314
BspPI GGATC 6 cut(s) 188, 1415, 1424, 1460, 1839, 2433
BspT104I TTCGAA 1 cut(s) 1963
BspT107I GGYRCC 4 cut(s) 16, 82, 1570, 2332
BspTNI GGTCTC 1 cut(s) 2891
BsrBI CCGCTC 1 cut(s) 214
BsrDI GCAATG 1 cut(s) 1155
BsrI ACTGG 8 cut(s) 624, 725, 1547, 2046, 2282, 2351, 2378, 2847
BssECI CCNNGG 6 cut(s) 12, 193, 640, 768, 1030, 1317
BssNAI GTATAC 2 cut(s) 1056, 1345
BssNI GRCGYC 6 cut(s) 17, 42, 54, 83, 119, 201
BssT1I CCWWGG 3 cut(s) 640, 768, 1030
Bst1107I GTATAC 2 cut(s) 1056, 1345
Bst2UI CCWGG 9 cut(s) 195, 351, 851, 1142, 1319, 1809, 1854, 2497, 2650
Bst4CI ACNGT 4 cut(s) 73, 616, 739, 2256
Bst6I CTCTTC 3 cut(s) 153, 337, 1133
BstACI GRCGYC 6 cut(s) 17, 42, 54, 83, 119, 201
BstAPI GCANNNNNTGC 1 cut(s) 2097
BstBAI YACGTR 2 cut(s) 275, 2735
BstBI TTCGAA 1 cut(s) 1963
BstC8I GCNNGC 9 cut(s) 466, 480, 575, 625, 1312, 1482, 1486, 2456, 2901
BstDEI CTNAG 9 cut(s) 65, 215, 251, 1294, 1306, 1415, 1770, 2629, 2919
BstEII GGTNACC 1 cut(s) 2609
BstF5I GGATG 9 cut(s) 385, 553, 1232, 1243, 1260, 1572, 1998, 2663, 2813
BstFNI CGCG 3 cut(s) 59, 1898, 2460
BstH2I RGCGCY 3 cut(s) 20, 86, 330
BstHHI GCGC 4 cut(s) 19, 85, 329, 2460
BstMAI GTCTC 2 cut(s) 1278, 2891
BstMWI GCNNNNNNNGC 9 cut(s) 25, 404, 539, 800, 1106, 1844, 1904, 2097, 2305
BstNI CCWGG 9 cut(s) 195, 351, 851, 1142, 1319, 1809, 1854, 2497, 2650
BstNSI RCATGY 5 cut(s) 577, 1003, 1351, 1484, 1636
BstPAI GACNNNNGTC 1 cut(s) 2883
BstPI GGTNACC 1 cut(s) 2609
BstSFI CTRYAG 3 cut(s) 184, 1826, 2148
BstSLI GKGCMC 3 cut(s) 663, 1476, 1738
BstUI CGCG 3 cut(s) 59, 1898, 2460
BstV1I GCAGC 5 cut(s) 385, 448, 494, 1157, 2487
BstV2I GAAGAC 3 cut(s) 1367, 1819, 1999
BstX2I RGATCY 4 cut(s) 180, 861, 1420, 2214
BstYI RGATCY 4 cut(s) 180, 861, 1420, 2214
BstZ17I GTATAC 2 cut(s) 1056, 1345
Bsu36I CCTNAGG 1 cut(s) 1770
BsuI GTATCC 1 cut(s) 989
BsuRI GGCC 5 cut(s) 849, 1878, 2018, 2211, 2385
BtrI CACGTC 1 cut(s) 172
BtsCI GGATG 9 cut(s) 385, 553, 1232, 1243, 1260, 1572, 1998, 2663, 2813
BtsI GCAGTG 1 cut(s) 2640
BtsIMutI CAGTG 7 cut(s) 750, 1070, 1920, 2039, 2252, 2640, 2854
BveI ACCTGC 1 cut(s) 416
Cac8I GCNNGC 9 cut(s) 466, 480, 575, 625, 1312, 1482, 1486, 2456, 2901
CaiI CAGNNNCTG 3 cut(s) 1141, 1808, 1859
CciI TCATGA 1 cut(s) 2863
CfoI GCGC 4 cut(s) 19, 85, 329, 2460
Cfr13I GGNCC 5 cut(s) 581, 722, 1321, 1492, 2209
CseI GACGC 4 cut(s) 48, 62, 209, 1904
Csp6I GTAC 6 cut(s) 145, 551, 617, 1245, 2030, 2579
CviQI GTAC 6 cut(s) 145, 551, 617, 1245, 2030, 2579
DdeI CTNAG 9 cut(s) 65, 215, 251, 1294, 1306, 1415, 1770, 2629, 2919
DinI GGCGCC 2 cut(s) 18, 84
DraIII CACNNNGTG 1 cut(s) 1282
DrdI GACNNNNNNGTC 2 cut(s) 41, 124
DriI GACNNNNNGTC 1 cut(s) 58
DseDI GACNNNNNNGTC 2 cut(s) 41, 124
EaeI YGGCCR 1 cut(s) 847
Eam1104I CTCTTC 3 cut(s) 153, 337, 1133
Eam1105I GACNNNNNGTC 1 cut(s) 58
EarI CTCTTC 3 cut(s) 153, 337, 1133
Eco130I CCWWGG 3 cut(s) 640, 768, 1030
Eco147I AGGCCT 2 cut(s) 1878, 2385
Eco31I GGTCTC 1 cut(s) 2891
Eco32I GATATC 1 cut(s) 2747
Eco47I GGWCC 4 cut(s) 581, 722, 1321, 1492
Eco57I CTGAAG 2 cut(s) 247, 1114
Eco81I CCTNAGG 1 cut(s) 1770
Eco91I GGTNACC 1 cut(s) 2609
EcoO109I RGGNCCY 2 cut(s) 581, 1492
EcoO65I GGTNACC 1 cut(s) 2609
EcoRII CCWGG 9 cut(s) 193, 349, 849, 1140, 1317, 1807, 1852, 2495, 2648
EcoRV GATATC 1 cut(s) 2747
EcoT14I CCWWGG 3 cut(s) 640, 768, 1030
EcoT22I ATGCAT 3 cut(s) 610, 796, 1655
EgeI GGCGCC 2 cut(s) 18, 84
EheI GGCGCC 2 cut(s) 18, 84
ErhI CCWWGG 3 cut(s) 640, 768, 1030
FaqI GGGAC 5 cut(s) 29, 53, 594, 1907, 2824
FauI CCCGC 3 cut(s) 130, 219, 2322
FblI GTMKAC 3 cut(s) 116, 1055, 1344
Fnu4HI GCNGC 7 cut(s) 20, 57, 399, 462, 483, 1146, 2501
FokI GGATG 9 cut(s) 392, 560, 1239, 1250, 1267, 1579, 2005, 2670, 2800
Fsp4HI GCNGC 7 cut(s) 20, 57, 399, 462, 483, 1146, 2501
FspBI CTAG 7 cut(s) 524, 717, 1016, 1059, 1311, 1556, 2835
GlaI GCGC 4 cut(s) 18, 84, 328, 2459
GluI GCNGC 7 cut(s) 20, 57, 399, 462, 483, 1146, 2501
GsaI CCCAGC 2 cut(s) 472, 1735
GsuI CTGGAG 4 cut(s) 423, 441, 2175, 2479
HaeII RGCGCY 3 cut(s) 20, 86, 330
HaeIII GGCC 5 cut(s) 849, 1878, 2018, 2211, 2385
HapII CCGG 4 cut(s) 14, 37, 178, 2521
HgaI GACGC 4 cut(s) 48, 62, 209, 1904
HhaI GCGC 4 cut(s) 19, 85, 329, 2460
Hin1I GRCGYC 6 cut(s) 17, 42, 54, 83, 119, 201
Hin6I GCGC 4 cut(s) 17, 83, 327, 2458
HinP1I GCGC 4 cut(s) 17, 83, 327, 2458
HincII GTYRAC 6 cut(s) 117, 298, 735, 874, 2556, 2626
HindII GTYRAC 6 cut(s) 117, 298, 735, 874, 2556, 2626
HinfI GANTC 4 cut(s) 755, 1338, 1937, 2831
HpaI GTTAAC 1 cut(s) 874
HpaII CCGG 4 cut(s) 14, 37, 178, 2521
HphI GGTGA 5 cut(s) 1076, 2326, 2428, 2488, 2704
HpyAV CCTTC 7 cut(s) 435, 673, 1196, 1456, 1584, 1889, 2729
HpyCH4III ACNGT 4 cut(s) 73, 616, 739, 2256
HpyCH4IV ACGT 7 cut(s) 42, 111, 119, 171, 274, 1779, 2734
HpyF10VI GCNNNNNNNGC 9 cut(s) 25, 404, 539, 800, 1106, 1844, 1904, 2097, 2305
HpyF3I CTNAG 9 cut(s) 65, 215, 251, 1294, 1306, 1415, 1770, 2629, 2919
HpySE526I ACGT 7 cut(s) 42, 111, 119, 171, 274, 1779, 2734
Hsp92I GRCGYC 6 cut(s) 17, 42, 54, 83, 119, 201
HspAI GCGC 4 cut(s) 17, 83, 327, 2458
KasI GGCGCC 2 cut(s) 16, 82
KspAI GTTAAC 1 cut(s) 874
LmnI GCTCC 6 cut(s) 239, 404, 422, 1319, 1710, 2523
Lsp1109I GCAGC 5 cut(s) 385, 448, 494, 1157, 2487
MaeI CTAG 7 cut(s) 524, 717, 1016, 1059, 1311, 1556, 2835
MaeII ACGT 7 cut(s) 42, 111, 119, 171, 274, 1779, 2734
MaeIII GTNAC 3 cut(s) 805, 976, 2609
MbiI CCGCTC 1 cut(s) 214
MfeI CAATTG 2 cut(s) 951, 1817
MflI RGATCY 4 cut(s) 180, 861, 1420, 2214
MhlI GDGCHC 6 cut(s) 409, 663, 1476, 1738, 2520, 2607
MlsI TGGCCA 1 cut(s) 849
MluNI TGGCCA 1 cut(s) 849
Mly113I GGCGCC 2 cut(s) 17, 83
MlyI GAGTC 1 cut(s) 2825
MmeI TCCRAC 5 cut(s) 69, 656, 1215, 1762, 2403
Mox20I TGGCCA 1 cut(s) 849
Mph1103I ATGCAT 3 cut(s) 610, 796, 1655
MroXI GAANNNNTTC 1 cut(s) 1116
MscI TGGCCA 1 cut(s) 849
MslI CAYNNNNRTG 3 cut(s) 273, 1219, 1479
Msp20I TGGCCA 1 cut(s) 849
MspA1I CMGCKG 2 cut(s) 803, 1427
MspI CCGG 4 cut(s) 14, 37, 178, 2521
MunI CAATTG 2 cut(s) 951, 1817
Mva1269I GAATGC 3 cut(s) 451, 796, 1653
MvaI CCWGG 9 cut(s) 195, 351, 851, 1142, 1319, 1809, 1854, 2497, 2650
MvnI CGCG 3 cut(s) 59, 1898, 2460
MwoI GCNNNNNNNGC 9 cut(s) 25, 404, 539, 800, 1106, 1844, 1904, 2097, 2305
NarI GGCGCC 2 cut(s) 17, 83
NciI CCSGG 3 cut(s) 14, 38, 2522
NheI GCTAGC 1 cut(s) 1310
NlaIV GGNNCC 9 cut(s) 18, 84, 191, 241, 582, 1493, 1572, 2334, 2699
NmeAIII GCCGAG 1 cut(s) 1994
NmuCI GTSAC 1 cut(s) 976
NsiI ATGCAT 3 cut(s) 610, 796, 1655
NspI RCATGY 5 cut(s) 577, 1003, 1351, 1484, 1636
NspV TTCGAA 1 cut(s) 1963
PaeI GCATGC 2 cut(s) 577, 1484
PagI TCATGA 1 cut(s) 2863
PceI AGGCCT 2 cut(s) 1878, 2385
PciI ACATGT 1 cut(s) 999
PcsI WCGNNNNNNNCGW 5 cut(s) 48, 108, 122, 125, 2740
PctI GAATGC 3 cut(s) 451, 796, 1653
PdmI GAANNNNTTC 1 cut(s) 1116
PfeI GAWTC 3 cut(s) 755, 1338, 1937
PflFI GACNNNGTC 1 cut(s) 121
PfoI TCCNGGA 2 cut(s) 349, 2520
PkrI GCNGC 7 cut(s) 21, 58, 400, 463, 484, 1147, 2502
PleI GAGTC 1 cut(s) 2825
PluTI GGCGCC 2 cut(s) 20, 86
PpsI GAGTC 1 cut(s) 2825
Ppu21I YACGTR 2 cut(s) 275, 2735
PpuMI RGGWCCY 2 cut(s) 581, 1492
PscI ACATGT 1 cut(s) 999
PshAI GACNNNNGTC 1 cut(s) 2883
PshBI ATTAAT 1 cut(s) 1217
Psp1406I AACGTT 1 cut(s) 1779
Psp5II RGGWCCY 2 cut(s) 581, 1492
Psp6I CCWGG 9 cut(s) 193, 349, 849, 1140, 1317, 1807, 1852, 2495, 2648
PspEI GGTNACC 1 cut(s) 2609
PspFI CCCAGC 2 cut(s) 468, 1731
PspGI CCWGG 9 cut(s) 193, 349, 849, 1140, 1317, 1807, 1852, 2495, 2648
PspN4I GGNNCC 9 cut(s) 18, 84, 191, 241, 582, 1493, 1572, 2334, 2699
PspPI GGNCC 5 cut(s) 581, 722, 1321, 1492, 2209
PspPPI RGGWCCY 2 cut(s) 581, 1492
PsrI GAACNNNNNNTAC 2 cut(s) 330, 362
PstNI CAGNNNCTG 3 cut(s) 1141, 1808, 1859
PsuI RGATCY 4 cut(s) 180, 861, 1420, 2214
PsyI GACNNNGTC 1 cut(s) 121
PvuII CAGCTG 2 cut(s) 803, 1427
RsaI GTAC 6 cut(s) 146, 552, 618, 1246, 2031, 2580
RsaNI GTAC 6 cut(s) 145, 551, 617, 1245, 2030, 2579
RseI CAYNNNNRTG 3 cut(s) 273, 1219, 1479
SalI GTCGAC 1 cut(s) 115
SatI GCNGC 7 cut(s) 20, 57, 399, 462, 483, 1146, 2501
Sau96I GGNCC 5 cut(s) 581, 722, 1321, 1492, 2209
SchI GAGTC 1 cut(s) 2825
SduI GDGCHC 6 cut(s) 409, 663, 1476, 1738, 2520, 2607
SfcI CTRYAG 3 cut(s) 184, 1826, 2148
SfoI GGCGCC 2 cut(s) 18, 84
SfuI TTCGAA 1 cut(s) 1963
SinI GGWCC 4 cut(s) 581, 722, 1321, 1492
SmiMI CAYNNNNRTG 3 cut(s) 273, 1219, 1479
SmlI CTYRAG 2 cut(s) 491, 2396
SmoI CTYRAG 2 cut(s) 491, 2396
SphI GCATGC 2 cut(s) 577, 1484
SseBI AGGCCT 2 cut(s) 1878, 2385
SsiI CCGC 9 cut(s) 20, 57, 137, 212, 714, 1508, 1564, 1628, 2329
SspDI GGCGCC 2 cut(s) 16, 82
SspI AATATT 1 cut(s) 654
SspMI CTAG 7 cut(s) 524, 717, 1016, 1059, 1311, 1556, 2835
StuI AGGCCT 2 cut(s) 1878, 2385
StyI CCWWGG 3 cut(s) 640, 768, 1030
TaaI ACNGT 4 cut(s) 73, 616, 739, 2256
TaiI ACGT 7 cut(s) 45, 114, 122, 174, 277, 1782, 2737
TaqI TCGA 4 cut(s) 116, 1381, 1873, 1963
TaqII GACCGA 2 cut(s) 2757, 2883
TatI WGTACW 3 cut(s) 616, 1244, 2578
TauI GCSGC 2 cut(s) 22, 59
TfiI GAWTC 3 cut(s) 755, 1338, 1937
TscAI CASTG 7 cut(s) 757, 1077, 1927, 2046, 2259, 2640, 2854
TseFI GTSAC 1 cut(s) 976
TseI GCWGC 5 cut(s) 398, 461, 482, 1145, 2500
Tsp45I GTSAC 1 cut(s) 976
TspGWI ACGGA 4 cut(s) 1439, 1492, 1697, 2879
TspRI CASTG 7 cut(s) 757, 1077, 1927, 2046, 2259, 2640, 2854
Tth111I GACNNNGTC 1 cut(s) 121
VneI GTGCAC 1 cut(s) 1472
VpaK11BI GGWCC 4 cut(s) 581, 722, 1321, 1492
VspI ATTAAT 1 cut(s) 1217
XapI RAATTY 2 cut(s) 811, 1620
XbaI TCTAGA 1 cut(s) 523
XceI RCATGY 5 cut(s) 577, 1003, 1351, 1484, 1636
XmiI GTMKAC 3 cut(s) 116, 1055, 1344
XmnI GAANNNNTTC 1 cut(s) 1116
XspI CTAG 7 cut(s) 524, 717, 1016, 1059, 1311, 1556, 2835
ZraI GACGTC 2 cut(s) 43, 120
Zsp2I ATGCAT 3 cut(s) 610, 796, 1655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.