Rroxscaffold_7G00209160

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0009109
Physical Location & Seq
Forward (+)
59437204 .. 59443095
5892 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00209160.1

Sequence Viewer

Length: 3309 bp
ATGGAGATCGCCCCGGCGCCGCCACAGCTTTCTGACCGGGACGTCCCAAACGACGCCGCGTCGCTAAGCACCGTCAAAACGGCGCCGTCGGAGAGCAATAACGAGGCGAACGTGTCGACGTCGTCGTCGTCGGTGGCGGCGAAGTACGACGATGAGGAGGAGGAGGAGGACGTGTGCCGGATCTGTAGGAACCCTGGCGACCCCGATAACCCGCTTAGGTATCCATGCGCGTGTAGCGGAAGCATCAAGTTCGTGCACCAGGATTGTCTCCTTCAGTGGCTTAATCACAGTAATGCTCGCCAATGCGAGGTTTGCAAGCATCCATTTTCCTTCTCTCCCGTTTATGCTGAGAATGCACCTGCTAGGCTTCCTTTTCAAGAGTTTGTAGTTGGGATGGCAATGAAAACTTGTCACGTTCTGCAATTTTTCCTGCGCCTTAGTTTTGTGCTTTCCGTTTGGCTCCTCATTATACCTTTCATCACATTTTGGATATGGCGGCTGGCTTTCGTGAGGAGTTTTGGTGAAGCTCAGAGATTATTCCTAAGTCATCTATCAACTACAGTCATTCTTACTGATTGTCTGCATGGGTTCCTACTTTCTGCTAGCATTGTGTTCATTTTTCTCGGAGCCACTTCTCTGAGGGATTACTTCAGGCATTTACGTGAGCTTGGAGGTCAAGATGTTGACAGAGAAGATGAAGCAGAAAGAAATGGCGCTCGTCCTGTAAGAAGAGTTCCTGGACAAGCTAATAGGAACTTTGTTGGGGATGCTAACGGGGAAGATGCTGCTGGAGCACAAGGGATTGCTGGAGCAGGTCAAATGCTTAGAAGGAATGCAGAAAATGTCGCTGCTCGCTGGGAGATGCAGGCAGCTCGTCTTGAGGCTCATGTTGAACAGATGTTTGATGGTCTAGACGATGCCGATGGTGCAGAGGATGTACCATTTGACGAGCTTGTGGGCATGCAGGGACCTGTATTTCATTTAGTGGAAAATGCATTTACTGTACTGGCAAGCAATATGATATTCCTTGGTATAGTAATATTTGTGCCCTTCTCGTTAGGTCGGATTATACTCTATCATTTATCTTGGCTTTTCTCCACCGCTAGTGGTCCAGTTTTGTCAACAGTCATGCCACTCACTGAATCAGCCCTTTCCTTGGCTAATATGACTTTGAAGAATGCATTAACAGCTGTAACAAATTTATCAACTGATAGCGAGAAAAGTGGTATGGTTGGCCAGGTTGCAGAGATCTTGAAAGTTAACATGAGTGGACTGAATGAGGTTTCGAACAATAATGCAAGTTCAACACTTTCAGCAGACTTCCTGAAAGGGGCAACAATTGGAACTTCAAGGCTCTCTGATGTGACAACTCTTGCTATTGGATACATGTTTATATTCTCCCTAGTTTTCTTCTACCTTGGTATTGTGGCTTTCATTCGGTATACTAGGGGTGAACCATTGACTATGGGGAGGTTTTATGGTATTGCTTCAATGGCAGAAACAATTCCATCTCTCTTCAGGCAGTTCCTGGCAGCAATGAGGCATTTAATGACAATGATTAAGGTCGCTTTCCTTCTGGTCATTGAACTTGGAGTGTTCCCATTAATGTGTGGATGGTGGTTGGATGTTTGTACTATAAGGATGTTTGGGAAGTCCATGTCTCACAGGGTGCAGTTCTTCTCAGCATCTCCCTTAGCTAGCTCCCTGGTCCATTGGGTTGTAGGAATCGTATACATGCTACAAATAAGCATATTCGTCAGTCTTCTTCGAGGGGTTCTGCGTAATGGAGTTCTTTATTTCCTTAGAGATCCAGCTGATCCAAACTACAATCCCTTCCGTGATCTCATTGATGATCCTGTGCACAAACATGCTCGCAGGGTCCTGTTATCCGTTGCGGTGTATGGGAGTTTAATTGTGATGCTGGTGTTTTTACCAGTTAAACTAGCTATGCGGATGGCACCTTCCATTTTTCCTCTTGACATTTCGGTATCTGACCCATTTACTGAAATTCCTGCGGACATGCTTCTATTTCAAATATGCATTCCATTTGCCATTGAGCATTTCAAATTACGGGCAACCATTAAATCCCTGCTCCGTTATTGGTTTACGGCAGTTGGCTGGGCACTTGGTTTAACAGATTTCTTACTACCCAGACCTGAGGATAACGTTGGACAGGAAAATGGAAATGCAGAGCCTGGAAGACAGGATAGACTACAGGATCAGGCTTTAGTGGCACTTCCAGGTGCTGATGAACCAAATCGAGGCCTTCTTGCATCAGGGGACACGAATGTTGCAGAGGAGTATGACACTGATGAACAATCTGATTCTGAAAGGTATAGCTTTGTGCTTCGAATTGTCCTCTTGTTGGTGGTGGCTTGGATGACTTTACTTGTCTTCAACTCGGCCTTGATAGTTGTACCAACATCATTGGGAAGAATAATTTTCAATGTCATTCCTTTCCTCCCTATAACTCATGGCATCAAGTGCAACGATTTATATGCTTTCATCATTGGAAGCTACATAATATGGACTGCTGTAGCTGGAGTTAGATATTCTATTGAACACATTAGAACAAAAAGGGTTGCAGTTCTATTGGGCCAGATCTGGAAGTGGTGTAGCATTGTCATTAAGAGTTCTGCACTGTTGTCAATATGGATTTTTGTCATTCCAGTATTGATCGGGTTGCTCTTTGAGCTTTTGGTGATTGTGCCGATGCGGGTGCCTGTTGATGAAAGCCCAGTATTCCTGCTGTATCAGGACTGGGCTTTAGGCCTTATTTTTCTCAAGATATGGACAAGATTGGTGATGTTGGATCACATGATGCCATTGGTAGATGAAAGCTGGCGCGTAAAGTTTGAAAGGGTGAGGGAAGATGGCTTCTCCAGGCTGCAAGGTCTTTGGGTGCTCCGGGAGATTGTATTCCCAATCATAATGAAGTTGTTGACTGCCCTGTGCGTGCCTTACGTACTTGCCAGAGGGTTATTTCCTGTGCTCGGTTACCCATTAGTAGTCAACTCAGCAGTGTATCGGTTTGCTTGGATAGGATGCCTGTGCTTTAGTCTGCTGTGCTTTTGTGCCAAGAGGTTCCATGTATGGTTCACCAACCTTCACAACTCTATACGTGATGACCGATATCTAATTGGTCGTAGGCTTCATAACTTTGGAGAAGATGTTGTAGAGAAGCAAAATGAGGCAGGGACATCCTCTGAATTGCAGGACTCTAGTCTTGAAGTCAGTGGTTTAATTCCTCATGACCGAGAGGCTGACGATGGTCTCCGTCAAAGGCGTGCTATCCAACATAATGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000209 GO:0001101 GO:0002020 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006508 GO:0006511 GO:0006629 GO:0006720 GO:0006725 GO:0006807 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009414 GO:0009415 GO:0009628 GO:0009698 GO:0009699 GO:0009893 GO:0009987 GO:0010025 GO:0010033 GO:0010035 GO:0010143 GO:0010166 GO:0010243 GO:0010345 GO:0010498 GO:0012505 GO:0016020 GO:0016021 GO:0016567 GO:0016740 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019787 GO:0019899 GO:0019941 GO:0030163 GO:0030176 GO:0030433 GO:0031224 GO:0031227 GO:0031624 GO:0031984 GO:0032446 GO:0032501 GO:0032502 GO:0033554 GO:0034976 GO:0036211 GO:0036503 GO:0042175 GO:0042221 GO:0042335 GO:0043085 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044390 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0048518 GO:0048856 GO:0050789 GO:0050790 GO:0050896 GO:0051341 GO:0051353 GO:0051603 GO:0051716 GO:0065007 GO:0065009 GO:0070647 GO:0070936 GO:0071704 GO:0098827 GO:0140096 GO:1900490 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901568 GO:1901570 GO:1901575 GO:1901576 GO:1901698 GO:1901700 GO:1990381 GO:2001215
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
RINGv

Protein Analysis

1102

Amino Acids

123.46

Weight (kDa)

5.83

Isoelectric Point (pI)

39.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 59 - 105 7.2e-17 RING-variant domain
MARCHF6_C PF23113 863 - 1041 7.8e-51 E3 ubiquitin-protein ligase MARCHF6-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 367
AasI GACNNNNNNGTC 2 cut(s) 41, 124
AatII GACGTC 2 cut(s) 45, 122
Acc36I ACCTGC 2 cut(s) 367, 803
AccB1I GGYRCC 4 cut(s) 16, 82, 1957, 2719
AccI GTMKAC 3 cut(s) 116, 1442, 1731
AccII CGCG 3 cut(s) 59, 230, 2847
AclI AACGTT 1 cut(s) 2166
AclWI GGATC 6 cut(s) 188, 1802, 1811, 1847, 2226, 2820
AcoI YGGCCR 1 cut(s) 1234
AcsI RAATTY 2 cut(s) 1198, 2007
AcuI CTGAAG 3 cut(s) 257, 634, 1501
AcyI GRCGYC 5 cut(s) 17, 42, 54, 83, 119
AdeI CACNNNGTG 1 cut(s) 1669
AfaI GTAC 6 cut(s) 146, 939, 1005, 1633, 2418, 2967
AfiI CCNNNNNNNGG 6 cut(s) 307, 1156, 1330, 2261, 2365, 2993
AflIII ACRYGT 3 cut(s) 111, 171, 1386
AhdI GACNNNNNGTC 1 cut(s) 58
AjiI CACGTC 1 cut(s) 172
AjnI CCWGG 9 cut(s) 193, 258, 736, 1236, 1527, 1704, 2194, 2239, 2882
Alw21I GWGCWC 5 cut(s) 258, 796, 1863, 2907, 2994
Alw26I GTCTC 3 cut(s) 272, 1665, 3278
Alw44I GTGCAC 2 cut(s) 254, 1859
AlwI GGATC 6 cut(s) 188, 1802, 1811, 1847, 2226, 2820
AlwNI CAGNNNCTG 3 cut(s) 1528, 2195, 2246
AoxI GGCC 5 cut(s) 1234, 2263, 2403, 2596, 2770
ApaLI GTGCAC 2 cut(s) 254, 1859
ApeKI GCWGC 5 cut(s) 785, 848, 869, 1532, 2887
ApoI RAATTY 2 cut(s) 1198, 2007
AseI ATTAAT 1 cut(s) 1604
Asp700I GAANNNNTTC 1 cut(s) 1503
AspLEI GCGC 6 cut(s) 19, 85, 230, 435, 716, 2847
AspS9I GGNCC 5 cut(s) 968, 1109, 1708, 1879, 2596
AsuC2I CCSGG 3 cut(s) 14, 38, 2909
AsuHPI GGTGA 6 cut(s) 533, 1463, 2713, 2815, 2875, 3091
AsuII TTCGAA 2 cut(s) 1286, 2350
AsuNHI GCTAGC 2 cut(s) 602, 1697
AvaII GGWCC 4 cut(s) 968, 1109, 1708, 1879
AxyI CCTNAGG 1 cut(s) 2157
BaeGI GKGCMC 4 cut(s) 258, 1050, 1863, 2125
BalI TGGCCA 1 cut(s) 1236
BanI GGYRCC 4 cut(s) 16, 82, 1957, 2719
BbsI GAAGAC 3 cut(s) 1754, 2206, 2386
Bbv12I GWGCWC 5 cut(s) 258, 796, 1863, 2907, 2994
BbvI GCAGC 5 cut(s) 772, 835, 881, 1544, 2874
BccI CCATC 8 cut(s) 388, 899, 917, 1516, 1608, 1948, 2867, 3263
BceAI ACGGC 3 cut(s) 70, 96, 2124
BcgI CGANNNNNNTGC 8 cut(s) 232, 266, 854, 888, 2480, 2514, 2701, 2735
BciT130I CCWGG 9 cut(s) 195, 260, 738, 1238, 1529, 1706, 2196, 2241, 2884
BciVI GTATCC 2 cut(s) 231, 1376
BcnI CCSGG 3 cut(s) 14, 38, 2909
BcoDI GTCTC 3 cut(s) 272, 1665, 3278
BfmI CTRYAG 4 cut(s) 184, 558, 2213, 2535
BfoI RGCGCY 3 cut(s) 20, 86, 717
BfuAI ACCTGC 2 cut(s) 367, 803
BfuI GTATCC 2 cut(s) 231, 1376
BglII AGATCT 2 cut(s) 1248, 2601
BisI GCNGC 9 cut(s) 20, 57, 138, 497, 786, 849, 870, 1533, 2888
BlpI GCTNAGC 1 cut(s) 65
BlsI GCNGC 9 cut(s) 21, 58, 139, 498, 787, 850, 871, 1534, 2889
Bme18I GGWCC 4 cut(s) 968, 1109, 1708, 1879
BmeRI GACNNNNNGTC 1 cut(s) 58
BmgBI CACGTC 1 cut(s) 172
BmgT120I GGNCC 5 cut(s) 968, 1109, 1708, 1879, 2596
BmrI ACTGGG 2 cut(s) 2732, 2770
BmtI GCTAGC 2 cut(s) 606, 1701
BmuI ACTGGG 2 cut(s) 2732, 2770
BoxI GACNNNNGTC 2 cut(s) 3222, 3270
BpiI GAAGAC 3 cut(s) 1754, 2206, 2386
BpmI CTGGAG 4 cut(s) 810, 828, 2562, 2866
Bpu10I CCTNAGC 2 cut(s) 215, 1693
Bpu1102I GCTNAGC 1 cut(s) 65
Bpu14I TTCGAA 2 cut(s) 1286, 2350
BpuEI CTTGAG 2 cut(s) 899, 2768
BpuMI CCSGG 3 cut(s) 14, 38, 2909
BsaAI YACGTR 3 cut(s) 662, 2965, 3122
BsaHI GRCGYC 5 cut(s) 17, 42, 54, 83, 119
BsaI GGTCTC 1 cut(s) 3278
BsaJI CCNNGG 6 cut(s) 12, 193, 1027, 1155, 1417, 1704
BsaXI ACNNNNNCTCC 6 cut(s) 158, 188, 505, 535, 3156, 3186
Bsc4I CCNNNNNNNGG 6 cut(s) 307, 1156, 1330, 2261, 2365, 2993
Bse1I ACTGG 6 cut(s) 1011, 1112, 1934, 2669, 2738, 2765
Bse21I CCTNAGG 1 cut(s) 2157
Bse3DI GCAATG 2 cut(s) 405, 1542
BseBI CCWGG 9 cut(s) 195, 260, 738, 1238, 1529, 1706, 2196, 2241, 2884
BseDI CCNNGG 6 cut(s) 12, 193, 1027, 1155, 1417, 1704
BseLI CCNNNNNNNGG 6 cut(s) 307, 1156, 1330, 2261, 2365, 2993
BseMI GCAATG 2 cut(s) 405, 1542
BseMII CTCAG 6 cut(s) 339, 542, 629, 1695, 2148, 3030
BseNI ACTGG 6 cut(s) 1011, 1112, 1934, 2669, 2738, 2765
BseRI GAGGAG 7 cut(s) 170, 173, 176, 179, 452, 526, 2312
BseSI GKGCMC 4 cut(s) 258, 1050, 1863, 2125
BseXI GCAGC 5 cut(s) 772, 835, 881, 1544, 2874
BseYI CCCAGC 2 cut(s) 855, 2118
BsgI GTGCAG 3 cut(s) 948, 1691, 2622
Bsh1236I CGCG 3 cut(s) 59, 230, 2847
BshFI GGCC 5 cut(s) 1236, 2265, 2405, 2598, 2772
BshNI GGYRCC 4 cut(s) 16, 82, 1957, 2719
BsiHKAI GWGCWC 5 cut(s) 258, 796, 1863, 2907, 2994
BsiSI CCGG 4 cut(s) 14, 37, 178, 2908
BslFI GGGAC 5 cut(s) 29, 53, 981, 2294, 3211
BslI CCNNNNNNNGG 6 cut(s) 307, 1156, 1330, 2261, 2365, 2993
BsmAI GTCTC 3 cut(s) 272, 1665, 3278
BsmFI GGGAC 5 cut(s) 29, 53, 981, 2294, 3211
BsmI GAATGC 4 cut(s) 358, 838, 1183, 2040
BsnI GGCC 5 cut(s) 1236, 2265, 2405, 2598, 2772
Bso31I GGTCTC 1 cut(s) 3278
Bsp119I TTCGAA 2 cut(s) 1286, 2350
Bsp1286I GDGCHC 7 cut(s) 258, 796, 1050, 1863, 2125, 2907, 2994
Bsp1720I GCTNAGC 1 cut(s) 65
BspANI GGCC 5 cut(s) 1236, 2265, 2405, 2598, 2772
BspCNI CTCAG 6 cut(s) 340, 541, 630, 1694, 2149, 3029
BspFNI CGCG 3 cut(s) 59, 230, 2847
BspHI TCATGA 1 cut(s) 3250
BspMI ACCTGC 2 cut(s) 367, 803
BspOI GCTAGC 2 cut(s) 606, 1701
BspPI GGATC 6 cut(s) 188, 1802, 1811, 1847, 2226, 2820
BspT104I TTCGAA 2 cut(s) 1286, 2350
BspT107I GGYRCC 4 cut(s) 16, 82, 1957, 2719
BspTNI GGTCTC 1 cut(s) 3278
BsrDI GCAATG 2 cut(s) 405, 1542
BsrI ACTGG 6 cut(s) 1011, 1112, 1934, 2669, 2738, 2765
BssECI CCNNGG 6 cut(s) 12, 193, 1027, 1155, 1417, 1704
BssNAI GTATAC 2 cut(s) 1443, 1732
BssNI GRCGYC 5 cut(s) 17, 42, 54, 83, 119
BssT1I CCWWGG 3 cut(s) 1027, 1155, 1417
Bst1107I GTATAC 2 cut(s) 1443, 1732
Bst2UI CCWGG 9 cut(s) 195, 260, 738, 1238, 1529, 1706, 2196, 2241, 2884
Bst4CI ACNGT 6 cut(s) 73, 290, 562, 1003, 1126, 2643
Bst6I CTCTTC 2 cut(s) 724, 1520
BstACI GRCGYC 5 cut(s) 17, 42, 54, 83, 119
BstAPI GCANNNNNTGC 1 cut(s) 2484
BstBAI YACGTR 3 cut(s) 662, 2965, 3122
BstBI TTCGAA 2 cut(s) 1286, 2350
BstEII GGTNACC 1 cut(s) 2996
BstFNI CGCG 3 cut(s) 59, 230, 2847
BstH2I RGCGCY 3 cut(s) 20, 86, 717
BstHHI GCGC 6 cut(s) 19, 85, 230, 435, 716, 2847
BstMAI GTCTC 3 cut(s) 272, 1665, 3278
BstNI CCWGG 9 cut(s) 195, 260, 738, 1238, 1529, 1706, 2196, 2241, 2884
BstNSI RCATGY 5 cut(s) 964, 1390, 1738, 1871, 2023
BstPAI GACNNNNGTC 2 cut(s) 3222, 3270
BstPI GGTNACC 1 cut(s) 2996
BstSFI CTRYAG 4 cut(s) 184, 558, 2213, 2535
BstSLI GKGCMC 4 cut(s) 258, 1050, 1863, 2125
BstSNI TACGTA 1 cut(s) 2965
BstUI CGCG 3 cut(s) 59, 230, 2847
BstV1I GCAGC 5 cut(s) 772, 835, 881, 1544, 2874
BstV2I GAAGAC 3 cut(s) 1754, 2206, 2386
BstX2I RGATCY 4 cut(s) 180, 1248, 1807, 2601
BstYI RGATCY 4 cut(s) 180, 1248, 1807, 2601
BstZ17I GTATAC 2 cut(s) 1443, 1732
Bsu36I CCTNAGG 1 cut(s) 2157
BsuI GTATCC 2 cut(s) 231, 1376
BsuRI GGCC 5 cut(s) 1236, 2265, 2405, 2598, 2772
BtrI CACGTC 1 cut(s) 172
BtsI GCAGTG 1 cut(s) 3027
BtsIMutI CAGTG 6 cut(s) 281, 1137, 2307, 2639, 3027, 3241
BveI ACCTGC 2 cut(s) 367, 803
CaiI CAGNNNCTG 3 cut(s) 1528, 2195, 2246
CciI TCATGA 1 cut(s) 3250
CfoI GCGC 6 cut(s) 19, 85, 230, 435, 716, 2847
Cfr13I GGNCC 5 cut(s) 968, 1109, 1708, 1879, 2596
CseI GACGC 2 cut(s) 48, 62
Csp6I GTAC 6 cut(s) 145, 938, 1004, 1632, 2417, 2966
CviQI GTAC 6 cut(s) 145, 938, 1004, 1632, 2417, 2966
DinI GGCGCC 2 cut(s) 18, 84
DraIII CACNNNGTG 1 cut(s) 1669
DrdI GACNNNNNNGTC 2 cut(s) 41, 124
DriI GACNNNNNGTC 1 cut(s) 58
DseDI GACNNNNNNGTC 2 cut(s) 41, 124
EaeI YGGCCR 1 cut(s) 1234
Eam1104I CTCTTC 2 cut(s) 724, 1520
Eam1105I GACNNNNNGTC 1 cut(s) 58
EarI CTCTTC 2 cut(s) 724, 1520
Eco105I TACGTA 1 cut(s) 2965
Eco130I CCWWGG 3 cut(s) 1027, 1155, 1417
Eco147I AGGCCT 2 cut(s) 2265, 2772
Eco31I GGTCTC 1 cut(s) 3278
Eco32I GATATC 1 cut(s) 3134
Eco47I GGWCC 4 cut(s) 968, 1109, 1708, 1879
Eco57I CTGAAG 3 cut(s) 257, 634, 1501
Eco81I CCTNAGG 1 cut(s) 2157
Eco91I GGTNACC 1 cut(s) 2996
EcoO109I RGGNCCY 2 cut(s) 968, 1879
EcoO65I GGTNACC 1 cut(s) 2996
EcoRII CCWGG 9 cut(s) 193, 258, 736, 1236, 1527, 1704, 2194, 2239, 2882
EcoRV GATATC 1 cut(s) 3134
EcoT14I CCWWGG 3 cut(s) 1027, 1155, 1417
EcoT22I ATGCAT 3 cut(s) 997, 1183, 2042
EgeI GGCGCC 2 cut(s) 18, 84
EheI GGCGCC 2 cut(s) 18, 84
ErhI CCWWGG 3 cut(s) 1027, 1155, 1417
FaqI GGGAC 5 cut(s) 29, 53, 981, 2294, 3211
FauI CCCGC 2 cut(s) 219, 2709
FblI GTMKAC 3 cut(s) 116, 1442, 1731
Fnu4HI GCNGC 9 cut(s) 20, 57, 138, 497, 786, 849, 870, 1533, 2888
Fsp4HI GCNGC 9 cut(s) 20, 57, 138, 497, 786, 849, 870, 1533, 2888
GlaI GCGC 6 cut(s) 18, 84, 229, 434, 715, 2846
GluI GCNGC 9 cut(s) 20, 57, 138, 497, 786, 849, 870, 1533, 2888
GsaI CCCAGC 2 cut(s) 859, 2122
GsuI CTGGAG 4 cut(s) 810, 828, 2562, 2866
HaeII RGCGCY 3 cut(s) 20, 86, 717
HaeIII GGCC 5 cut(s) 1236, 2265, 2405, 2598, 2772
HapII CCGG 4 cut(s) 14, 37, 178, 2908
HgaI GACGC 2 cut(s) 48, 62
HhaI GCGC 6 cut(s) 19, 85, 230, 435, 716, 2847
Hin1I GRCGYC 5 cut(s) 17, 42, 54, 83, 119
Hin6I GCGC 6 cut(s) 17, 83, 228, 433, 714, 2845
HinP1I GCGC 6 cut(s) 17, 83, 228, 433, 714, 2845
HincII GTYRAC 6 cut(s) 117, 685, 1122, 1261, 2943, 3013
HindII GTYRAC 6 cut(s) 117, 685, 1122, 1261, 2943, 3013
HinfI GANTC 4 cut(s) 1142, 1725, 2324, 3218
HpaI GTTAAC 1 cut(s) 1261
HpaII CCGG 4 cut(s) 14, 37, 178, 2908
HphI GGTGA 6 cut(s) 533, 1463, 2713, 2815, 2875, 3091
Hpy99I CGWCG 9 cut(s) 56, 64, 91, 121, 124, 127, 130, 133, 152
HpyAV CCTTC 9 cut(s) 281, 340, 822, 1060, 1583, 1843, 1971, 2276, 3116
HpyCH4III ACNGT 6 cut(s) 73, 290, 562, 1003, 1126, 2643
HpyCH4IV ACGT 9 cut(s) 42, 111, 119, 171, 414, 661, 2166, 2964, 3121
HpySE526I ACGT 9 cut(s) 42, 111, 119, 171, 414, 661, 2166, 2964, 3121
Hsp92I GRCGYC 5 cut(s) 17, 42, 54, 83, 119
HspAI GCGC 6 cut(s) 17, 83, 228, 433, 714, 2845
KasI GGCGCC 2 cut(s) 16, 82
KspAI GTTAAC 1 cut(s) 1261
LmnI GCTCC 7 cut(s) 465, 626, 791, 809, 1706, 2097, 2910
Lsp1109I GCAGC 5 cut(s) 772, 835, 881, 1544, 2874
MaeII ACGT 9 cut(s) 42, 111, 119, 171, 414, 661, 2166, 2964, 3121
MaeIII GTNAC 4 cut(s) 410, 1192, 1363, 2996
MfeI CAATTG 1 cut(s) 1338
MflI RGATCY 4 cut(s) 180, 1248, 1807, 2601
MhlI GDGCHC 7 cut(s) 258, 796, 1050, 1863, 2125, 2907, 2994
MlsI TGGCCA 1 cut(s) 1236
MluNI TGGCCA 1 cut(s) 1236
Mly113I GGCGCC 2 cut(s) 17, 83
MlyI GAGTC 1 cut(s) 3212
MmeI TCCRAC 5 cut(s) 69, 1043, 1602, 2149, 2790
Mox20I TGGCCA 1 cut(s) 1236
Mph1103I ATGCAT 3 cut(s) 997, 1183, 2042
MroXI GAANNNNTTC 1 cut(s) 1503
MscI TGGCCA 1 cut(s) 1236
MslI CAYNNNNRTG 5 cut(s) 229, 291, 660, 1606, 1866
Msp20I TGGCCA 1 cut(s) 1236
MspA1I CMGCKG 2 cut(s) 1190, 1814
MspI CCGG 4 cut(s) 14, 37, 178, 2908
MunI CAATTG 1 cut(s) 1338
Mva1269I GAATGC 4 cut(s) 358, 838, 1183, 2040
MvaI CCWGG 9 cut(s) 195, 260, 738, 1238, 1529, 1706, 2196, 2241, 2884
MvnI CGCG 3 cut(s) 59, 230, 2847
NarI GGCGCC 2 cut(s) 17, 83
NciI CCSGG 3 cut(s) 14, 38, 2909
NheI GCTAGC 2 cut(s) 602, 1697
NmeAIII GCCGAG 1 cut(s) 2381
NmuCI GTSAC 2 cut(s) 410, 1363
NsiI ATGCAT 3 cut(s) 997, 1183, 2042
NspI RCATGY 5 cut(s) 964, 1390, 1738, 1871, 2023
NspV TTCGAA 2 cut(s) 1286, 2350
PaeI GCATGC 1 cut(s) 964
PagI TCATGA 1 cut(s) 3250
PaqCI CACCTGC 1 cut(s) 367
PceI AGGCCT 2 cut(s) 2265, 2772
PciI ACATGT 1 cut(s) 1386
PcsI WCGNNNNNNNCGW 6 cut(s) 48, 108, 122, 125, 137, 3127
PctI GAATGC 4 cut(s) 358, 838, 1183, 2040
PdmI GAANNNNTTC 1 cut(s) 1503
PfeI GAWTC 3 cut(s) 1142, 1725, 2324
PflFI GACNNNGTC 1 cut(s) 121
PfoI TCCNGGA 2 cut(s) 736, 2907
PkrI GCNGC 9 cut(s) 21, 58, 139, 498, 787, 850, 871, 1534, 2889
PleI GAGTC 1 cut(s) 3212
PluTI GGCGCC 2 cut(s) 20, 86
PpsI GAGTC 1 cut(s) 3212
Ppu21I YACGTR 3 cut(s) 662, 2965, 3122
PpuMI RGGWCCY 2 cut(s) 968, 1879
PscI ACATGT 1 cut(s) 1386
PshAI GACNNNNGTC 2 cut(s) 3222, 3270
PshBI ATTAAT 1 cut(s) 1604
Psp1406I AACGTT 1 cut(s) 2166
Psp5II RGGWCCY 2 cut(s) 968, 1879
Psp6I CCWGG 9 cut(s) 193, 258, 736, 1236, 1527, 1704, 2194, 2239, 2882
PspEI GGTNACC 1 cut(s) 2996
PspFI CCCAGC 2 cut(s) 855, 2118
PspGI CCWGG 9 cut(s) 193, 258, 736, 1236, 1527, 1704, 2194, 2239, 2882
PspPI GGNCC 5 cut(s) 968, 1109, 1708, 1879, 2596
PspPPI RGGWCCY 2 cut(s) 968, 1879
PsrI GAACNNNNNNTAC 2 cut(s) 717, 749
PstNI CAGNNNCTG 3 cut(s) 1528, 2195, 2246
PsuI RGATCY 4 cut(s) 180, 1248, 1807, 2601
PsyI GACNNNGTC 1 cut(s) 121
PvuII CAGCTG 2 cut(s) 1190, 1814
RsaI GTAC 6 cut(s) 146, 939, 1005, 1633, 2418, 2967
RsaNI GTAC 6 cut(s) 145, 938, 1004, 1632, 2417, 2966
RseI CAYNNNNRTG 5 cut(s) 229, 291, 660, 1606, 1866
SalI GTCGAC 1 cut(s) 115
SatI GCNGC 9 cut(s) 20, 57, 138, 497, 786, 849, 870, 1533, 2888
Sau96I GGNCC 5 cut(s) 968, 1109, 1708, 1879, 2596
SchI GAGTC 1 cut(s) 3212
SduI GDGCHC 7 cut(s) 258, 796, 1050, 1863, 2125, 2907, 2994
SfcI CTRYAG 4 cut(s) 184, 558, 2213, 2535
SfoI GGCGCC 2 cut(s) 18, 84
SfuI TTCGAA 2 cut(s) 1286, 2350
SinI GGWCC 4 cut(s) 968, 1109, 1708, 1879
SmiMI CAYNNNNRTG 5 cut(s) 229, 291, 660, 1606, 1866
SmlI CTYRAG 2 cut(s) 878, 2783
SmoI CTYRAG 2 cut(s) 878, 2783
SnaBI TACGTA 1 cut(s) 2965
SphI GCATGC 1 cut(s) 964
SseBI AGGCCT 2 cut(s) 2265, 2772
SspDI GGCGCC 2 cut(s) 16, 82
SspI AATATT 1 cut(s) 1041
StuI AGGCCT 2 cut(s) 2265, 2772
StyI CCWWGG 3 cut(s) 1027, 1155, 1417
TaaI ACNGT 6 cut(s) 73, 290, 562, 1003, 1126, 2643
TaiI ACGT 9 cut(s) 45, 114, 122, 174, 417, 664, 2169, 2967, 3124
TaqI TCGA 5 cut(s) 116, 1286, 1768, 2260, 2350
TaqII GACCGA 2 cut(s) 3144, 3270
TatI WGTACW 2 cut(s) 1003, 1631
TauI GCSGC 4 cut(s) 22, 59, 140, 499
TfiI GAWTC 3 cut(s) 1142, 1725, 2324
TscAI CASTG 6 cut(s) 281, 1144, 2314, 2646, 3027, 3241
TseFI GTSAC 2 cut(s) 410, 1363
TseI GCWGC 5 cut(s) 785, 848, 869, 1532, 2887
Tsp45I GTSAC 2 cut(s) 410, 1363
TspGWI ACGGA 5 cut(s) 442, 1826, 1879, 2084, 3266
TspRI CASTG 6 cut(s) 281, 1144, 2314, 2646, 3027, 3241
Tth111I GACNNNGTC 1 cut(s) 121
VneI GTGCAC 2 cut(s) 254, 1859
VpaK11BI GGWCC 4 cut(s) 968, 1109, 1708, 1879
VspI ATTAAT 1 cut(s) 1604
XapI RAATTY 2 cut(s) 1198, 2007
XbaI TCTAGA 1 cut(s) 910
XceI RCATGY 5 cut(s) 964, 1390, 1738, 1871, 2023
XmiI GTMKAC 3 cut(s) 116, 1442, 1731
XmnI GAANNNNTTC 1 cut(s) 1503
ZraI GACGTC 2 cut(s) 43, 120
Zsp2I ATGCAT 3 cut(s) 997, 1183, 2042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.