MD00G1138200.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0009109
Physical Location & Seq
Forward (+)
29893035 .. 29898544
5510 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1138200.v1.1.491

Sequence Viewer

Length: 3303 bp
ATGGAGATCACCCCGTCCCCGCCGCCGTCCGCTGACCAGGACGTCCCAACCGACACCGTCAAGACGTCGTCGTCTCAGGAGAACGAAGCGAGCGCCGTGGCGGCGACGGTCAAGTACGACGATGAGGAAGAGGAGGAGGACGTGTGCCGGATCTGTAGAAACACGGGCGACGCCGATAACCCGCTGCGGTACCCGTGCGCTTGTAGCGGAAGCATCAAGTTTGTGCACCAGGATTGCCTCCTGCAGTGGCTTAATCACAGCAATGCTCGCCAATGCGAGGTTTGCAAGCATGCATTTTCCTTCTCTCCTGTATATGCTGAGAATGCCCCTGCAAGGCTTCCTTTTCAAGAGTTTGTAGTTGGGATGGCAATGAAAACTTGCCATGTTCTGCAATTCCTTCTGCGGCTTAGTTTTGTGCTTTCTGTTTGGCTTCTCATTATACCTTTTATCACATTTTGGATATGGCGGTTGGCTCTTGTGAGGAGTTTTGGTGAAGCCCAGAGATTATTCCTTAGTCATTTATCCACTACAGTCATCCTTACCGATTGTCTGCATGGGTTCCTACTTTCTGCTAGCATTGTGTTTATTTTTCTTGGGGCCACTTCTCTGAGGGATTACTTCAGGCATTTACGTGAGCTTGGAGGACAGGATGCTGACAGAGAAGATGAAGGGGAAGGAAATGGTGCCCGTGCTGCAAGGAGAGCTCCTGGACAAGCTAACAGGAACTTTGTTGGTGATGTGAATGGGGAAGATGCAGGAGCACATGGGATTGCTGGAGCAGGTCAAATGATTAGGAGGAATGCAGAAAATGTTGCTGCTCGGTGGGAGATGCAGGCAGCTCGTCTTGAAGCTCATGTGGAGCAGATGTTTGATGGTCTGGATGATGCTGATGGCGCCGAGGATGTACCGTTTGATGAGCTTGTGGGCATGCAGGGACCTGTATTTCATTTAGTTGAAAATGCGTTCACTGTAAATTTTGAGGTTCTGGCCAGCAATATGATATTCCTTGGTGTAGTAATCTTTGTGCCTTTCTCAATAGGTCGGATTATACTCTATCGTTTGTTTTGGATTTTCTCCACCGCTACTGGTCCAGTATTGTCAACAGTCATGCCACTTACAGAATCAGCCCTTTCCTTAGCAAATGTAACATTGAAGAATGCAGTAACAGCTGTAACGAATGTATCATCTGAAAGCCAACAAAGTGGTATGGTAGGACAGGTTGAAGAGATCCTGAAAGCAAACATGAGTGGACTAAATGAGGTTTCAAACAATGTAAGCTCACCACTATCAGCAGACTTCTTGAAAGGGGCAACACTTGGGACATCAAGGCTGTCTGATGTGACAACTCTTGCTGTTGGATACATGTTTGTGTTCTCCCTAGTGTTCTTCTACCTGGGCATTGTTGCTTTGATTCAGTACACTAGGGGTGAATCATTGACTATGGGAAGGTTCTATGGTATTGCTTCTATGGCAGAGACAATTTCATCTCTCTTCAGGCAGTTCCTGGCAGCAATGAGGCATTTGATGACTATGATTAAGGTTGCTTTTCTTCTAGTCCTTGAACTGGGGGTATTTCCCTTGATGTGTGGATGGTGGCTAGATGTTTGTACTATAAGGATGTTCGGGAAGTCCATGTCTCACAGGGTTCAATTCTTCTCAGCTTCTCCCTTAGCCAGTTCATTGGTCCATTGGGTGGTTGGAATTATATACATGCTACAAATAAGCATATTTGTCAGTCTTCTTCGAGGGGTCCTGCGTAATGGAGTTCTTTATTTCCTTAGAGATCCAGCTGATCCTAACTATAATCCATTCCGTGATCTTATTGACGATCCGGTGCACAAACATGCTCGCAGGGTCCTCTTATCTATTGCGGTGTATGGGAGTTTAATTGTGATGCTGGTGTTTTTACCGGTTAAACTAGCTATGCGGATGGCACCTTCCATTTTCCCTCTTGACATCTTGGTGTCCGACCCATTTACTGAAATTCCTGCAGACATGCTTCTTTTTCAAATATGCATTCCATTCGCCACTGAGCATTTCAAATTACGGACAACGATTAAATCCCTCCTCTGTTATTGGTTTACAACAGTTGGCTGGGCTCTTGGTTTAACTGATTTCTTACTGCCCAGATCTGAGGACAATGGTGCACAGGAAAATGGAAATGCAGAGCCAGGAAGACAGGATAGAGTACAGGTTCAACTGGGGGTACAGGATCAGGCTTTGGTGGCACTTCCAGGTGCTGATGATCCAAATGGAGTTATTCTTGCATCAGGAGACTCAAATGTCGCAGAAGAGTATGATACGGATGAACAATCTGATTCTGAAAGGTACAGCTTTGTGCTTCGCATTGTACTTTTGTTGGTGGTGGCTTGGATGACGTTACTTGTCTTCAACTCTGCCTTGATAGTCGTACCAACCTCACTTGGAAGGGCAATTTTCAATGTCATTCCTTTTCTCCCTATAACACATGGAATCAAGTGCAATGATTTGTATGCTTTCATCATTGGAAGCTACATAATATGGACTGCTGTAGCCGGATTTAGATATTCCATCGAGCATATTAGAACCAAAAGGGTTGCAGTTCTGTTGGGCCAGATCTTCAAGTGGTGTGCCATTGTTGTCAAGAGTTCTGTGCTTTTGTCAATATGGATTTTTGTCATTCCAGTATTGATTGGGCTGCTTTTTGAGCTTTTGGTGATCGTTCCAATGCGTGTGCCTGTTGATGAGAGCCCGGTATTCCTGCTTTACCAGGACTGGGCTTTAGGCCTTATTTTTCTCAAGATATGGACCAGACTGGTTATGTTGGATCACATGATGCCACTGATGGATGAAACCTGGCGAGTAAAGTTTGAAAGGGTGAGGGAAGACGGGTTCTCCAGGCTGCAAGGTCTTTGGGTGCTTCGTGAAATCGTATTCCCAATCATTATGAAGTTGTTGACTGCCCTGTGCGTGCCTTATGTACTTGCCAGAGGGCTATTTCCTGTTCTCGGTTACCCATTAGTGGTGAACTCAGCAGTTTATCGGTTTGCCTGGCTGGGATGCCTTTGCTTTAGCCTGTTGTGCTTTTGCGCCAGGAGATTCCATGTCTGGTTCACAAACCTTCACAACTCAATACGTGATGACCGGTACCTAATTGGTCGAAGGCTTCATAACTTTGGGGAAGCCGTCGAAGAGAAGCAAAACGAAGCAGGGACATCCTCGGAAGTGCAGGACTCTAACTACGAGACCAACGGGTTAATCCGATACGACCGAGAAGTTGATATAGGACTCCGACTAAGGCGTGTTAACCAAGTCGATGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000209 GO:0001101 GO:0002020 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006508 GO:0006511 GO:0006629 GO:0006720 GO:0006725 GO:0006807 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009414 GO:0009415 GO:0009628 GO:0009698 GO:0009699 GO:0009893 GO:0009987 GO:0010025 GO:0010033 GO:0010035 GO:0010143 GO:0010166 GO:0010243 GO:0010345 GO:0010498 GO:0012505 GO:0016020 GO:0016021 GO:0016567 GO:0016740 GO:0019222 GO:0019438 GO:0019538 GO:0019748 GO:0019787 GO:0019899 GO:0019941 GO:0030163 GO:0030176 GO:0030433 GO:0031224 GO:0031227 GO:0031624 GO:0031984 GO:0032446 GO:0032501 GO:0032502 GO:0033554 GO:0034976 GO:0036211 GO:0036503 GO:0042175 GO:0042221 GO:0042335 GO:0043085 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044390 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0048518 GO:0048856 GO:0050789 GO:0050790 GO:0050896 GO:0051341 GO:0051353 GO:0051603 GO:0051716 GO:0065007 GO:0065009 GO:0070647 GO:0070936 GO:0071704 GO:0098827 GO:0140096 GO:1900490 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901568 GO:1901570 GO:1901575 GO:1901576 GO:1901698 GO:1901700 GO:1990381 GO:2001215
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
RINGv

Protein Analysis

1101

Amino Acids

123.26

Weight (kDa)

5.5

Isoelectric Point (pI)

37.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 49 - 95 7.7e-17 RING-variant domain
MARCHF6_C PF23113 862 - 1039 2.7e-50 E3 ubiquitin-protein ligase MARCHF6-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 41, 70, 2282
AatII GACGTC 2 cut(s) 45, 68
Acc36I ACCTGC 1 cut(s) 770
Acc65I GGTACC 2 cut(s) 189, 3126
AccB1I GGYRCC 5 cut(s) 189, 683, 893, 1933, 3126
AccB7I CCANNNNNTGG 1 cut(s) 1693
AclWI GGATC 8 cut(s) 158, 1222, 1778, 1787, 1823, 2220, 2240, 2814
AcoI YGGCCR 1 cut(s) 987
AcsI RAATTY 2 cut(s) 973, 1983
AcuI CTGAAG 2 cut(s) 604, 1477
AcyI GRCGYC 4 cut(s) 42, 65, 171, 894
AfiI CCNNNNNNNGG 7 cut(s) 277, 333, 1564, 1693, 2755, 2987, 3001
AflIII ACRYGT 2 cut(s) 141, 1362
AgeI ACCGGT 2 cut(s) 1909, 3123
AjiI CACGTC 1 cut(s) 142
AloI GAACNNNNNNTCC 2 cut(s) 2855, 2887
Alw21I GWGCWC 5 cut(s) 228, 706, 763, 1839, 2149
Alw26I GTCTC 5 cut(s) 78, 1469, 1641, 2268, 3218
Alw44I GTGCAC 3 cut(s) 224, 1835, 2145
AlwI GGATC 8 cut(s) 158, 1222, 1778, 1787, 1823, 2220, 2240, 2814
AlwNI CAGNNNCTG 2 cut(s) 1504, 2240
AoxI GGCC 4 cut(s) 597, 987, 2590, 2764
ApaLI GTGCAC 3 cut(s) 224, 1835, 2145
ApeKI GCWGC 7 cut(s) 184, 692, 815, 836, 1508, 2677, 2881
ApoI RAATTY 2 cut(s) 973, 1983
AsiGI ACCGGT 2 cut(s) 1909, 3123
Asp718I GGTACC 2 cut(s) 189, 3126
AspLEI GCGC 4 cut(s) 95, 200, 896, 3071
AspS9I GGNCC 8 cut(s) 597, 935, 1088, 1684, 1750, 1855, 2590, 2787
AsuC2I CCSGG 1 cut(s) 2732
AsuHPI GGTGA 7 cut(s) 503, 746, 1272, 1439, 2707, 2869, 3016
AsuNHI GCTAGC 1 cut(s) 572
AvaII GGWCC 6 cut(s) 935, 1088, 1684, 1750, 1855, 2787
BaeGI GKGCMC 4 cut(s) 228, 688, 1839, 2149
BaeI ACNNNNGTAYC 2 cut(s) 1164, 1197
BalI TGGCCA 1 cut(s) 989
BanI GGYRCC 5 cut(s) 189, 683, 893, 1933, 3126
BanII GRGCYC 3 cut(s) 706, 2101, 2732
BbsI GAAGAC 4 cut(s) 1730, 2182, 2380, 2871
Bbv12I GWGCWC 5 cut(s) 228, 706, 763, 1839, 2149
BbvI GCAGC 7 cut(s) 171, 679, 802, 848, 1520, 2664, 2868
BccI CCATC 7 cut(s) 358, 866, 884, 1584, 1924, 2558, 2818
BceAI ACGGC 3 cut(s) 10, 80, 3149
BcgI CGANNNNNNTGC 4 cut(s) 821, 855, 2005, 2039
BciVI GTATCC 1 cut(s) 1352
BcnI CCSGG 1 cut(s) 2732
BcoDI GTCTC 5 cut(s) 78, 1469, 1641, 2268, 3218
BfaI CTAG 6 cut(s) 573, 1379, 1422, 1553, 1598, 1919
BfmI CTRYAG 5 cut(s) 154, 242, 528, 1989, 2529
BfoI RGCGCY 2 cut(s) 96, 897
BfuAI ACCTGC 1 cut(s) 770
BfuI GTATCC 1 cut(s) 1352
BglI GCCNNNNNGGC 1 cut(s) 101
BglII AGATCT 2 cut(s) 2129, 2595
Bme18I GGWCC 6 cut(s) 935, 1088, 1684, 1750, 1855, 2787
BmgBI CACGTC 1 cut(s) 142
BmgT120I GGNCC 8 cut(s) 597, 935, 1088, 1684, 1750, 1855, 2590, 2787
BmrI ACTGGG 3 cut(s) 1574, 2210, 2764
BmsI GCATC 9 cut(s) 222, 640, 742, 819, 874, 1884, 2276, 2805, 3029
BmtI GCTAGC 1 cut(s) 576
BmuI ACTGGG 3 cut(s) 1574, 2210, 2764
BpiI GAAGAC 4 cut(s) 1730, 2182, 2380, 2871
BpmI CTGGAG 2 cut(s) 795, 2860
Bpu10I CCTNAGC 2 cut(s) 1135, 1669
BpuEI CTTGAG 1 cut(s) 2762
BpuMI CCSGG 1 cut(s) 2732
BsaAI YACGTR 2 cut(s) 632, 3116
BsaHI GRCGYC 4 cut(s) 42, 65, 171, 894
BsaI GGTCTC 1 cut(s) 3218
BsaJI CCNNGG 5 cut(s) 96, 897, 1006, 1393, 3198
BsaWI WCCGGW 3 cut(s) 1831, 1909, 3123
BsaXI ACNNNNNCTCC 8 cut(s) 128, 158, 475, 505, 851, 881, 2858, 2888
Bsc4I CCNNNNNNNGG 7 cut(s) 277, 333, 1564, 1693, 2755, 2987, 3001
Bse118I RCCGGY 2 cut(s) 1909, 3123
Bse1I ACTGG 8 cut(s) 1090, 1091, 1569, 1674, 2205, 2663, 2759, 2799
Bse3DI GCAATG 4 cut(s) 268, 375, 1518, 2488
BseDI CCNNGG 5 cut(s) 96, 897, 1006, 1393, 3198
BseLI CCNNNNNNNGG 7 cut(s) 277, 333, 1564, 1693, 2755, 2987, 3001
BseMI GCAATG 4 cut(s) 268, 375, 1518, 2488
BseMII CTCAG 7 cut(s) 89, 309, 599, 1671, 2022, 2124, 3024
BseNI ACTGG 8 cut(s) 1090, 1091, 1569, 1674, 2205, 2663, 2759, 2799
BseRI GAGGAG 4 cut(s) 146, 149, 496, 2057
BseSI GKGCMC 4 cut(s) 228, 688, 1839, 2149
BseXI GCAGC 7 cut(s) 171, 679, 802, 848, 1520, 2664, 2868
BseYI CCCAGC 2 cut(s) 2094, 3034
BsgI GTGCAG 1 cut(s) 3227
Bsh1285I CGRYCG 1 cut(s) 3250
BshFI GGCC 4 cut(s) 599, 989, 2592, 2766
BshNI GGYRCC 5 cut(s) 189, 683, 893, 1933, 3126
BshTI ACCGGT 2 cut(s) 1909, 3123
BsiEI CGRYCG 1 cut(s) 3250
BsiHKAI GWGCWC 5 cut(s) 228, 706, 763, 1839, 2149
BsiSI CCGG 6 cut(s) 148, 1832, 1910, 2535, 2732, 3124
BslFI GGGAC 4 cut(s) 29, 948, 1333, 3205
BslI CCNNNNNNNGG 7 cut(s) 277, 333, 1564, 1693, 2755, 2987, 3001
BsmAI GTCTC 5 cut(s) 78, 1469, 1641, 2268, 3218
BsmBI CGTCTC 1 cut(s) 78
BsmFI GGGAC 4 cut(s) 29, 948, 1333, 3205
BsmI GAATGC 4 cut(s) 328, 805, 1162, 2016
BsnI GGCC 4 cut(s) 599, 989, 2592, 2766
Bso31I GGTCTC 1 cut(s) 3218
Bsp1286I GDGCHC 8 cut(s) 228, 688, 706, 763, 1839, 2101, 2149, 2732
BspANI GGCC 4 cut(s) 599, 989, 2592, 2766
BspCNI CTCAG 7 cut(s) 88, 310, 600, 1670, 2023, 2125, 3023
BspMAI CTGCAG 2 cut(s) 246, 1993
BspMI ACCTGC 1 cut(s) 770
BspOI GCTAGC 1 cut(s) 576
BspPI GGATC 8 cut(s) 158, 1222, 1778, 1787, 1823, 2220, 2240, 2814
BspT107I GGYRCC 5 cut(s) 189, 683, 893, 1933, 3126
BspTNI GGTCTC 1 cut(s) 3218
BsrDI GCAATG 4 cut(s) 268, 375, 1518, 2488
BsrFI RCCGGY 2 cut(s) 1909, 3123
BsrI ACTGG 8 cut(s) 1090, 1091, 1569, 1674, 2205, 2663, 2759, 2799
BssAI RCCGGY 2 cut(s) 1909, 3123
BssECI CCNNGG 5 cut(s) 96, 897, 1006, 1393, 3198
BssNI GRCGYC 4 cut(s) 42, 65, 171, 894
BssT1I CCWWGG 1 cut(s) 1006
Bst4CI ACNGT 7 cut(s) 58, 109, 532, 909, 970, 1105, 2089
Bst6I CTCTTC 5 cut(s) 123, 1218, 1496, 2286, 3165
BstACI GRCGYC 4 cut(s) 42, 65, 171, 894
BstBAI YACGTR 2 cut(s) 632, 3116
BstDSI CCRYGG 1 cut(s) 96
BstEII GGTNACC 1 cut(s) 2990
BstH2I RGCGCY 2 cut(s) 96, 897
BstHHI GCGC 4 cut(s) 95, 200, 896, 3071
BstMAI GTCTC 5 cut(s) 78, 1469, 1641, 2268, 3218
BstMCI CGRYCG 1 cut(s) 3250
BstNSI RCATGY 6 cut(s) 293, 931, 1366, 1714, 1847, 1999
BstPI GGTNACC 1 cut(s) 2990
BstSFI CTRYAG 5 cut(s) 154, 242, 528, 1989, 2529
BstSLI GKGCMC 4 cut(s) 228, 688, 1839, 2149
BstV1I GCAGC 7 cut(s) 171, 679, 802, 848, 1520, 2664, 2868
BstV2I GAAGAC 4 cut(s) 1730, 2182, 2380, 2871
BstX2I RGATCY 5 cut(s) 150, 1227, 1783, 2129, 2595
BstXI CCANNNNNNTGG 2 cut(s) 1202, 1681
BstYI RGATCY 5 cut(s) 150, 1227, 1783, 2129, 2595
BsuI GTATCC 1 cut(s) 1352
BsuRI GGCC 4 cut(s) 599, 989, 2592, 2766
BtgI CCRYGG 1 cut(s) 96
BtrI CACGTC 1 cut(s) 142
BtsI GCAGTG 1 cut(s) 251
BtsIMutI CAGTG 4 cut(s) 251, 966, 2028, 2819
BveI ACCTGC 1 cut(s) 770
CaiI CAGNNNCTG 2 cut(s) 1504, 2240
CfoI GCGC 4 cut(s) 95, 200, 896, 3071
Cfr10I RCCGGY 2 cut(s) 1909, 3123
Cfr13I GGNCC 8 cut(s) 597, 935, 1088, 1684, 1750, 1855, 2590, 2787
CseI GACGC 1 cut(s) 179
CspAI ACCGGT 2 cut(s) 1909, 3123
DinI GGCGCC 1 cut(s) 895
DrdI GACNNNNNNGTC 3 cut(s) 41, 70, 2282
DseDI GACNNNNNNGTC 3 cut(s) 41, 70, 2282
EaeI YGGCCR 1 cut(s) 987
Eam1104I CTCTTC 5 cut(s) 123, 1218, 1496, 2286, 3165
EarI CTCTTC 5 cut(s) 123, 1218, 1496, 2286, 3165
Ecl136II GAGCTC 1 cut(s) 704
Eco130I CCWWGG 1 cut(s) 1006
Eco147I AGGCCT 1 cut(s) 2766
Eco24I GRGCYC 3 cut(s) 706, 2101, 2732
Eco31I GGTCTC 1 cut(s) 3218
Eco47I GGWCC 6 cut(s) 935, 1088, 1684, 1750, 1855, 2787
Eco53kI GAGCTC 1 cut(s) 704
Eco57I CTGAAG 2 cut(s) 604, 1477
Eco91I GGTNACC 1 cut(s) 2990
EcoICRI GAGCTC 1 cut(s) 704
EcoO109I RGGNCCY 3 cut(s) 935, 1750, 1855
EcoO65I GGTNACC 1 cut(s) 2990
EcoT14I CCWWGG 1 cut(s) 1006
EcoT22I ATGCAT 2 cut(s) 295, 2018
EcoT38I GRGCYC 3 cut(s) 706, 2101, 2732
EgeI GGCGCC 1 cut(s) 895
EheI GGCGCC 1 cut(s) 895
ErhI CCWWGG 1 cut(s) 1006
Esp3I CGTCTC 1 cut(s) 78
FalI AAGNNNNNCTT 2 cut(s) 325, 357
FaqI GGGAC 4 cut(s) 29, 948, 1333, 3205
FauI CCCGC 2 cut(s) 27, 189
FriOI GRGCYC 3 cut(s) 706, 2101, 2732
FspBI CTAG 6 cut(s) 573, 1379, 1422, 1553, 1598, 1919
GlaI GCGC 4 cut(s) 94, 199, 895, 3070
GsaI CCCAGC 2 cut(s) 2098, 3038
GsuI CTGGAG 2 cut(s) 795, 2860
HaeII RGCGCY 2 cut(s) 96, 897
HaeIII GGCC 4 cut(s) 599, 989, 2592, 2766
HapII CCGG 6 cut(s) 148, 1832, 1910, 2535, 2732, 3124
HgaI GACGC 1 cut(s) 179
HhaI GCGC 4 cut(s) 95, 200, 896, 3071
Hin1I GRCGYC 4 cut(s) 42, 65, 171, 894
Hin6I GCGC 4 cut(s) 93, 198, 894, 3069
HinP1I GCGC 4 cut(s) 93, 198, 894, 3069
HincII GTYRAC 3 cut(s) 1101, 2937, 3286
HindII GTYRAC 3 cut(s) 1101, 2937, 3286
HinfI GANTC 9 cut(s) 1121, 1411, 1430, 2276, 2318, 2472, 3078, 3212, 3267
HpaI GTTAAC 1 cut(s) 3286
HpaII CCGG 6 cut(s) 148, 1832, 1910, 2535, 2732, 3124
HphI GGTGA 7 cut(s) 503, 746, 1272, 1439, 2707, 2869, 3016
Hpy99I CGWCG 6 cut(s) 70, 73, 109, 122, 173, 3170
HpyAV CCTTC 9 cut(s) 310, 407, 662, 668, 1440, 1947, 2421, 3110, 3135
HpyCH4III ACNGT 7 cut(s) 58, 109, 532, 909, 970, 1105, 2089
HpyCH4IV ACGT 6 cut(s) 42, 65, 141, 631, 2378, 3115
HpySE526I ACGT 6 cut(s) 42, 65, 141, 631, 2378, 3115
Hsp92I GRCGYC 4 cut(s) 42, 65, 171, 894
HspAI GCGC 4 cut(s) 93, 198, 894, 3069
KasI GGCGCC 1 cut(s) 893
KpnI GGTACC 2 cut(s) 193, 3130
KspAI GTTAAC 1 cut(s) 3286
LmnI GCTCC 4 cut(s) 709, 758, 776, 859
Lsp1109I GCAGC 7 cut(s) 171, 679, 802, 848, 1520, 2664, 2868
LweI GCATC 9 cut(s) 222, 640, 742, 819, 874, 1884, 2276, 2805, 3029
MaeI CTAG 6 cut(s) 573, 1379, 1422, 1553, 1598, 1919
MaeII ACGT 6 cut(s) 42, 65, 141, 631, 2378, 3115
MaeIII GTNAC 6 cut(s) 1144, 1162, 1171, 1339, 2379, 2990
MflI RGATCY 5 cut(s) 150, 1227, 1783, 2129, 2595
MhlI GDGCHC 8 cut(s) 228, 688, 706, 763, 1839, 2101, 2149, 2732
MlsI TGGCCA 1 cut(s) 989
MluNI TGGCCA 1 cut(s) 989
Mly113I GGCGCC 1 cut(s) 894
MlyI GAGTC 3 cut(s) 2270, 3206, 3261
MmeI TCCRAC 6 cut(s) 1022, 1336, 1678, 1992, 2784, 3295
Mox20I TGGCCA 1 cut(s) 989
Mph1103I ATGCAT 2 cut(s) 295, 2018
MscI TGGCCA 1 cut(s) 989
MseI TTAA 8 cut(s) 252, 1536, 1886, 1914, 2058, 2108, 3236, 3285
MslI CAYNNNNRTG 5 cut(s) 261, 630, 1367, 1842, 1961
Msp20I TGGCCA 1 cut(s) 989
MspA1I CMGCKG 4 cut(s) 32, 184, 1169, 1790
MspI CCGG 6 cut(s) 148, 1832, 1910, 2535, 2732, 3124
Mva1269I GAATGC 4 cut(s) 328, 805, 1162, 2016
NarI GGCGCC 1 cut(s) 894
NciI CCSGG 1 cut(s) 2732
NheI GCTAGC 1 cut(s) 572
NmeAIII GCCGAG 1 cut(s) 922
NmuCI GTSAC 1 cut(s) 1339
NsiI ATGCAT 2 cut(s) 295, 2018
NspI RCATGY 6 cut(s) 293, 931, 1366, 1714, 1847, 1999
PaeI GCATGC 2 cut(s) 293, 931
PceI AGGCCT 1 cut(s) 2766
PciI ACATGT 1 cut(s) 1362
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PctI GAATGC 4 cut(s) 328, 805, 1162, 2016
PfeI GAWTC 6 cut(s) 1121, 1411, 1430, 2318, 2472, 3078
PflFI GACNNNGTC 2 cut(s) 56, 67
PflMI CCANNNNNTGG 1 cut(s) 1693
PfoI TCCNGGA 1 cut(s) 706
PinAI ACCGGT 2 cut(s) 1909, 3123
PleI GAGTC 3 cut(s) 2270, 3206, 3261
PluTI GGCGCC 1 cut(s) 897
PpsI GAGTC 3 cut(s) 2270, 3206, 3261
Ppu21I YACGTR 2 cut(s) 632, 3116
PpuMI RGGWCCY 3 cut(s) 935, 1750, 1855
PscI ACATGT 1 cut(s) 1362
Psp124BI GAGCTC 1 cut(s) 706
Psp5II RGGWCCY 3 cut(s) 935, 1750, 1855
PspEI GGTNACC 1 cut(s) 2990
PspFI CCCAGC 2 cut(s) 2094, 3034
PspPI GGNCC 8 cut(s) 597, 935, 1088, 1684, 1750, 1855, 2590, 2787
PspPPI RGGWCCY 3 cut(s) 935, 1750, 1855
PstI CTGCAG 2 cut(s) 246, 1993
PstNI CAGNNNCTG 2 cut(s) 1504, 2240
PsuI RGATCY 5 cut(s) 150, 1227, 1783, 2129, 2595
PsyI GACNNNGTC 2 cut(s) 56, 67
PvuII CAGCTG 2 cut(s) 1169, 1790
RseI CAYNNNNRTG 5 cut(s) 261, 630, 1367, 1842, 1961
SacI GAGCTC 1 cut(s) 706
SaqAI TTAA 8 cut(s) 252, 1536, 1886, 1914, 2058, 2108, 3236, 3285
Sau96I GGNCC 8 cut(s) 597, 935, 1088, 1684, 1750, 1855, 2590, 2787
SchI GAGTC 3 cut(s) 2270, 3206, 3261
SduI GDGCHC 8 cut(s) 228, 688, 706, 763, 1839, 2101, 2149, 2732
SfaNI GCATC 9 cut(s) 222, 640, 742, 819, 874, 1884, 2276, 2805, 3029
SfcI CTRYAG 5 cut(s) 154, 242, 528, 1989, 2529
SfoI GGCGCC 1 cut(s) 895
SinI GGWCC 6 cut(s) 935, 1088, 1684, 1750, 1855, 2787
SmiMI CAYNNNNRTG 5 cut(s) 261, 630, 1367, 1842, 1961
SmlI CTYRAG 1 cut(s) 2777
SmoI CTYRAG 1 cut(s) 2777
SphI GCATGC 2 cut(s) 293, 931
SseBI AGGCCT 1 cut(s) 2766
SspDI GGCGCC 1 cut(s) 893
SspMI CTAG 6 cut(s) 573, 1379, 1422, 1553, 1598, 1919
SstI GAGCTC 1 cut(s) 706
StuI AGGCCT 1 cut(s) 2766
StyI CCWWGG 1 cut(s) 1006
TaaI ACNGT 7 cut(s) 58, 109, 532, 909, 970, 1105, 2089
TaiI ACGT 6 cut(s) 45, 68, 144, 634, 2381, 3118
TaqI TCGA 5 cut(s) 1744, 2553, 3139, 3168, 3294
TaqII GACCGA 1 cut(s) 3264
TatI WGTACW 5 cut(s) 1416, 1607, 2188, 2350, 2959
TauI GCSGC 3 cut(s) 25, 104, 406
TfiI GAWTC 6 cut(s) 1121, 1411, 1430, 2318, 2472, 3078
Tru1I TTAA 8 cut(s) 252, 1536, 1886, 1914, 2058, 2108, 3236, 3285
Tru9I TTAA 8 cut(s) 252, 1536, 1886, 1914, 2058, 2108, 3236, 3285
TscAI CASTG 4 cut(s) 251, 973, 2035, 2826
TseFI GTSAC 1 cut(s) 1339
TseI GCWGC 7 cut(s) 184, 692, 815, 836, 1508, 2677, 2881
Tsp45I GTSAC 1 cut(s) 1339
TspGWI ACGGA 3 cut(s) 1802, 2062, 2318
TspRI CASTG 4 cut(s) 251, 973, 2035, 2826
Tth111I GACNNNGTC 2 cut(s) 56, 67
Van91I CCANNNNNTGG 1 cut(s) 1693
VneI GTGCAC 3 cut(s) 224, 1835, 2145
VpaK11BI GGWCC 6 cut(s) 935, 1088, 1684, 1750, 1855, 2787
XapI RAATTY 2 cut(s) 973, 1983
XceI RCATGY 6 cut(s) 293, 931, 1366, 1714, 1847, 1999
XcmI CCANNNNNNNNNTGG 1 cut(s) 1694
XspI CTAG 6 cut(s) 573, 1379, 1422, 1553, 1598, 1919
ZraI GACGTC 2 cut(s) 43, 66
Zsp2I ATGCAT 2 cut(s) 295, 2018
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.