FvH4_2g05781
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009649
Physical Location & Seq
Reverse (-)
4730674 .. 4733739
3066 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g05781.t1

Sequence Viewer

Length: 1260 bp
ATGGAATCTTCAACCCTCGTCGCTCTACTCTCCTCCGTCTCCCAACTCCTCCTCCTCCTCGTCCTCTCTCCCAACCCTTCTTCCTCCTCCTCCTCCTTCCCCGCCGTCCACCACCTCCTCTCCTCCCAAGAACTCGCCGCCACCCTCTCCCTCCTCTCCCTCTCCCGCAAACGCAAACGCGCCCGCCTCTCTTCCCCAACCCAACTCCTCCCTCGCTCCCCCGACTCCTTCAAGACCCATTTCCGCATGACCTCCTCCACCTTCGAGTGGCTCTGCTCCTTACTTGAGCCCTTACTCGAGTGCCGCGACCCCGTCGGCTCCTCTCTCAATCTCTCCGCCGACCTCCGCCTCGGTATCGGCCTCTTCCGTCTCGCCACCGGAGCCAACTACCATGTCATCTCCCAACAATTCCGGGTTTCCGAGACCGTCGCCAGATTCTGCTCCAAGCAACTCTGCCGTGTGCTCTGTACCAATTACCGCTTCTGGATTGAGTTTCCCGATAAATCGGAGCTTCAATCGGTGTCGGCCGGGTTCGAAGCGCACACCGGGCTGCCCAATTGCTGCGGTGTGATCGACTGCGCCCGGTTTAGGGTGGTTAGAGATAATGGAGTTGAACAAGAGAGAGTTGCAGCTCAGATCATGGTGGATGCTACGTCGAGAATTCTCAGCATTGTGGCTGGGTTTAGGGGGAGTAAGAGTGATGATATGGTTTTGAAGTGTTCTACATTGTATGCGGATATTGAGCGTGGCGAGTTACTGAATTTGGAGGCGGTGAGTGTGGATGGGGTGCCGGTGAATCAGTACTTGGTTGGAGGTGGAGGGTACCCTTTGCTTCCGTGGCTTATGGTGCCGTTTGTTGATGCAATGCCAGGGTCGAATGAGGAGCAGTTCAATGTGGCACACAGTAGGATGAGGCTTTCGGGGCTTAGAGTGGTTGATAGTTTGAAGAATTGGGGGGTTTTGAGTAGGCCGATTCGCGAGGAAATGAAGATGGCTGTTGCTTATATTGGTGCTTGTGCCATACTTCATAATGGTTTGTTGATGAGGGAGGACTACTCGGCAATGTCTGGTGGGTTGGATGATTACTCTTTGTATGATCAGAGTTCGAGGTATTATAGGGACGATACGAGTTTGGAGGAGAGTTCGATTGAGAGGAGGGCTTCTGTTATAAGAAATGCATTGGCTACAAAAGCTAAAGAGTTTCAAGAATCAATTTTATCGGCCAATTCTAGCTCTTTGGCATCATTGGAATCAACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

420

Amino Acids

46.16

Weight (kDa)

6.41

Isoelectric Point (pI)

51.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 209 - 344 1.4e-15 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1169
Acc65I GGTACC 1 cut(s) 822
AccB1I GGYRCC 3 cut(s) 787, 822, 847
AccII CGCG 3 cut(s) 180, 306, 978
AcoI YGGCCR 2 cut(s) 525, 1221
AcsI RAATTY 2 cut(s) 660, 760
AfaI GTAC 3 cut(s) 469, 803, 824
AfiI CCNNNNNNNGG 3 cut(s) 267, 588, 589
AgsI TTSAA 8 cut(s) 12, 232, 515, 614, 715, 892, 946, 1205
AjnI CCWGG 1 cut(s) 868
AjuI GAANNNNNNNTTGG 4 cut(s) 464, 496, 788, 820
AluBI AGCT 4 cut(s) 511, 632, 1193, 1233
AluI AGCT 4 cut(s) 511, 632, 1193, 1233
Alw21I GWGCWC 1 cut(s) 465
Alw26I GTCTC 3 cut(s) 43, 374, 416
AlwNI CAGNNNCTG 1 cut(s) 438
Ama87I CYCGRG 1 cut(s) 296
AoxI GGCC 4 cut(s) 358, 525, 968, 1221
ApeKI GCWGC 3 cut(s) 550, 561, 629
ApoI RAATTY 2 cut(s) 660, 760
Asp718I GGTACC 1 cut(s) 822
AspLEI GCGC 3 cut(s) 182, 541, 581
AsuC2I CCSGG 4 cut(s) 413, 529, 547, 583
AsuHPI GGTGA 2 cut(s) 784, 805
AsuII TTCGAA 1 cut(s) 534
AvaI CYCGRG 1 cut(s) 296
BanI GGYRCC 3 cut(s) 787, 822, 847
BanII GRGCYC 1 cut(s) 291
Bbv12I GWGCWC 1 cut(s) 465
BbvI GCAGC 3 cut(s) 537, 548, 641
BccI CCATC 2 cut(s) 776, 985
BceAI ACGGC 3 cut(s) 89, 441, 835
BciT130I CCWGG 1 cut(s) 870
BclI TGATCA 1 cut(s) 1096
BcnI CCSGG 4 cut(s) 413, 529, 547, 583
BcoDI GTCTC 3 cut(s) 43, 374, 416
BfaI CTAG 2 cut(s) 1230, 1258
BisI GCNGC 5 cut(s) 138, 304, 551, 562, 630
BlsI GCNGC 5 cut(s) 139, 305, 552, 563, 631
BmcAI AGTACT 1 cut(s) 803
Bme1390I CCNGG 5 cut(s) 413, 529, 547, 583, 870
BmeT110I CYCGRG 1 cut(s) 296
BmiI GGNNCC 5 cut(s) 319, 382, 789, 824, 849
BmrFI CCNGG 5 cut(s) 413, 529, 547, 583, 870
BmsI GCATC 3 cut(s) 637, 850, 1250
BplI GAGNNNNNCTC 2 cut(s) 1040, 1072
Bpu14I TTCGAA 1 cut(s) 534
BpuEI CTTGAG 1 cut(s) 305
BpuMI CCSGG 4 cut(s) 413, 529, 547, 583
BsaI GGTCTC 1 cut(s) 416
BsaJI CCNNGG 3 cut(s) 349, 836, 869
BsaWI WCCGGW 1 cut(s) 377
BsaXI ACNNNNNCTCC 6 cut(s) 36, 66, 104, 134, 682, 712
Bsc4I CCNNNNNNNGG 3 cut(s) 267, 588, 589
Bse118I RCCGGY 1 cut(s) 790
Bse3DI GCAATG 2 cut(s) 870, 1068
BseBI CCWGG 1 cut(s) 870
BseDI CCNNGG 3 cut(s) 349, 836, 869
BseGI GGATG 4 cut(s) 652, 787, 915, 1084
BseLI CCNNNNNNNGG 3 cut(s) 267, 588, 589
BseMI GCAATG 2 cut(s) 870, 1068
BseMII CTCAG 2 cut(s) 647, 679
BseX3I CGGCCG 1 cut(s) 525
BseXI GCAGC 3 cut(s) 537, 548, 641
BseYI CCCAGC 1 cut(s) 677
Bsh1236I CGCG 3 cut(s) 180, 306, 978
Bsh1285I CGRYCG 1 cut(s) 528
BshFI GGCC 4 cut(s) 360, 527, 970, 1223
BshNI GGYRCC 3 cut(s) 787, 822, 847
BsiEI CGRYCG 1 cut(s) 528
BsiHKAI GWGCWC 1 cut(s) 465
BsiHKCI CYCGRG 1 cut(s) 296
BsiSI CCGG 6 cut(s) 378, 412, 528, 546, 583, 791
BslFI GGGAC 1 cut(s) 1133
BslI CCNNNNNNNGG 3 cut(s) 267, 588, 589
BsmAI GTCTC 3 cut(s) 43, 374, 416
BsmBI CGTCTC 2 cut(s) 43, 374
BsmFI GGGAC 1 cut(s) 1133
BsnI GGCC 4 cut(s) 360, 527, 970, 1223
Bso31I GGTCTC 1 cut(s) 416
BsoBI CYCGRG 1 cut(s) 296
Bsp119I TTCGAA 1 cut(s) 534
Bsp1286I GDGCHC 2 cut(s) 291, 465
Bsp143I GATC 3 cut(s) 570, 636, 1096
Bsp68I TCGCGA 1 cut(s) 978
BspANI GGCC 4 cut(s) 360, 527, 970, 1223
BspCNI CTCAG 2 cut(s) 646, 678
BspFNI CGCG 3 cut(s) 180, 306, 978
BspLI GGNNCC 5 cut(s) 319, 382, 789, 824, 849
BspT104I TTCGAA 1 cut(s) 534
BspT107I GGYRCC 3 cut(s) 787, 822, 847
BspTNI GGTCTC 1 cut(s) 416
BsrDI GCAATG 2 cut(s) 870, 1068
BsrFI RCCGGY 1 cut(s) 790
BssAI RCCGGY 1 cut(s) 790
BssECI CCNNGG 3 cut(s) 349, 836, 869
BssMI GATC 3 cut(s) 570, 636, 1096
Bst2UI CCWGG 1 cut(s) 870
Bst4CI ACNGT 2 cut(s) 427, 905
Bst6I CTCTTC 2 cut(s) 196, 368
BstBI TTCGAA 1 cut(s) 534
BstC8I GCNNGC 1 cut(s) 184
BstDEI CTNAG 3 cut(s) 633, 665, 926
BstDSI CCRYGG 1 cut(s) 836
BstF5I GGATG 4 cut(s) 652, 787, 915, 1084
BstFNI CGCG 3 cut(s) 180, 306, 978
BstHHI GCGC 3 cut(s) 182, 541, 581
BstKTI GATC 3 cut(s) 573, 639, 1099
BstMAI GTCTC 3 cut(s) 43, 374, 416
BstMBI GATC 3 cut(s) 570, 636, 1096
BstMCI CGRYCG 1 cut(s) 528
BstMWI GCNNNNNNNGC 6 cut(s) 380, 547, 838, 847, 922, 1190
BstNI CCWGG 1 cut(s) 870
BstSCI CCNGG 5 cut(s) 411, 527, 545, 581, 868
BstUI CGCG 3 cut(s) 180, 306, 978
BstV1I GCAGC 3 cut(s) 537, 548, 641
BstZI CGGCCG 1 cut(s) 525
BsuRI GGCC 4 cut(s) 360, 527, 970, 1223
BtgI CCRYGG 1 cut(s) 836
BtsCI GGATG 4 cut(s) 652, 787, 915, 1084
BtuMI TCGCGA 1 cut(s) 978
Cac8I GCNNGC 1 cut(s) 184
CaiI CAGNNNCTG 1 cut(s) 438
CfoI GCGC 3 cut(s) 182, 541, 581
Cfr10I RCCGGY 1 cut(s) 790
Csp6I GTAC 3 cut(s) 468, 802, 823
CviAII CATG 3 cut(s) 247, 392, 640
CviQI GTAC 3 cut(s) 468, 802, 823
DdeI CTNAG 3 cut(s) 633, 665, 926
DpnI GATC 3 cut(s) 572, 638, 1098
DpnII GATC 3 cut(s) 570, 636, 1096
EaeI YGGCCR 2 cut(s) 525, 1221
EagI CGGCCG 1 cut(s) 525
Eam1104I CTCTTC 2 cut(s) 196, 368
EarI CTCTTC 2 cut(s) 196, 368
EciI GGCGGA 2 cut(s) 325, 335
EclXI CGGCCG 1 cut(s) 525
Eco24I GRGCYC 1 cut(s) 291
Eco31I GGTCTC 1 cut(s) 416
Eco52I CGGCCG 1 cut(s) 525
Eco88I CYCGRG 1 cut(s) 296
EcoRI GAATTC 1 cut(s) 660
EcoRII CCWGG 1 cut(s) 868
EcoT22I ATGCAT 1 cut(s) 1180
EcoT38I GRGCYC 1 cut(s) 291
Esp3I CGTCTC 2 cut(s) 43, 374
FaeI CATG 3 cut(s) 250, 395, 643
FaqI GGGAC 1 cut(s) 1133
FatI CATG 3 cut(s) 246, 391, 639
FauI CCCGC 3 cut(s) 109, 173, 191
FbaI TGATCA 1 cut(s) 1096
Fnu4HI GCNGC 5 cut(s) 138, 304, 551, 562, 630
FokI GGATG 4 cut(s) 659, 794, 922, 1091
FriOI GRGCYC 1 cut(s) 291
Fsp4HI GCNGC 5 cut(s) 138, 304, 551, 562, 630
FspBI CTAG 2 cut(s) 1230, 1258
GlaI GCGC 3 cut(s) 181, 540, 580
GluI GCNGC 5 cut(s) 138, 304, 551, 562, 630
GsaI CCCAGC 1 cut(s) 681
HaeIII GGCC 4 cut(s) 360, 527, 970, 1223
HapII CCGG 6 cut(s) 378, 412, 528, 546, 583, 791
HhaI GCGC 3 cut(s) 182, 541, 581
Hin1II CATG 3 cut(s) 250, 395, 643
Hin6I GCGC 3 cut(s) 180, 539, 579
HinP1I GCGC 3 cut(s) 180, 539, 579
HinfI GANTC 7 cut(s) 5, 224, 435, 796, 973, 1208, 1250
HpaII CCGG 6 cut(s) 378, 412, 528, 546, 583, 791
HphI GGTGA 2 cut(s) 784, 805
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 4 cut(s) 421, 508, 636, 1101
Hpy188III TCNNGA 6 cut(s) 232, 484, 497, 657, 977, 1205
Hpy8I GTNNAC 1 cut(s) 109
Hpy99I CGWCG 4 cut(s) 23, 317, 431, 658
HpyAV CCTTC 4 cut(s) 87, 106, 238, 271
HpyCH4III ACNGT 2 cut(s) 427, 905
HpyCH4IV ACGT 1 cut(s) 653
HpyCH4V TGCA 3 cut(s) 629, 863, 1178
HpyF10VI GCNNNNNNNGC 6 cut(s) 380, 547, 838, 847, 922, 1190
HpyF3I CTNAG 3 cut(s) 633, 665, 926
HpySE526I ACGT 1 cut(s) 653
Hsp92II CATG 3 cut(s) 250, 395, 643
HspAI GCGC 3 cut(s) 180, 539, 579
KpnI GGTACC 1 cut(s) 826
Ksp22I TGATCA 1 cut(s) 1096
Kzo9I GATC 3 cut(s) 570, 636, 1096
LmnI GCTCC 7 cut(s) 221, 281, 323, 380, 446, 508, 883
Lsp1109I GCAGC 3 cut(s) 537, 548, 641
LweI GCATC 3 cut(s) 637, 850, 1250
MaeI CTAG 2 cut(s) 1230, 1258
MaeII ACGT 1 cut(s) 653
MaeIII GTNAC 1 cut(s) 753
MalI GATC 3 cut(s) 572, 638, 1098
MboI GATC 3 cut(s) 570, 636, 1096
MboII GAAGA 5 cut(s) 72, 183, 355, 958, 1000
MfeI CAATTG 1 cut(s) 556
MhlI GDGCHC 2 cut(s) 291, 465
MluCI AATT 8 cut(s) 407, 472, 556, 660, 760, 949, 1212, 1225
MlyI GAGTC 1 cut(s) 218
MmeI TCCRAC 2 cut(s) 790, 1056
Mph1103I ATGCAT 1 cut(s) 1180
MspI CCGG 6 cut(s) 378, 412, 528, 546, 583, 791
MspR9I CCNGG 5 cut(s) 413, 529, 547, 583, 870
MunI CAATTG 1 cut(s) 556
MvaI CCWGG 1 cut(s) 870
MvnI CGCG 3 cut(s) 180, 306, 978
MwoI GCNNNNNNNGC 6 cut(s) 380, 547, 838, 847, 922, 1190
NciI CCSGG 4 cut(s) 413, 529, 547, 583
NdeII GATC 3 cut(s) 570, 636, 1096
NlaIII CATG 3 cut(s) 250, 395, 643
NlaIV GGNNCC 5 cut(s) 319, 382, 789, 824, 849
NmeAIII GCCGAG 1 cut(s) 1037
NruI TCGCGA 1 cut(s) 978
NsiI ATGCAT 1 cut(s) 1180
NspV TTCGAA 1 cut(s) 534
PaeR7I CTCGAG 1 cut(s) 296
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PfeI GAWTC 6 cut(s) 5, 435, 796, 973, 1208, 1250
PflFI GACNNNGTC 1 cut(s) 311
PkrI GCNGC 5 cut(s) 139, 305, 552, 563, 631
PleI GAGTC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 218
PsiI TTATAA 1 cut(s) 1169
Psp6I CCWGG 1 cut(s) 868
PspFI CCCAGC 1 cut(s) 677
PspGI CCWGG 1 cut(s) 868
PspN4I GGNNCC 5 cut(s) 319, 382, 789, 824, 849
PspXI VCTCGAGB 1 cut(s) 296
PstNI CAGNNNCTG 1 cut(s) 438
PsyI GACNNNGTC 1 cut(s) 311
RruI TCGCGA 1 cut(s) 978
RsaI GTAC 3 cut(s) 469, 803, 824
RsaNI GTAC 3 cut(s) 468, 802, 823
SatI GCNGC 5 cut(s) 138, 304, 551, 562, 630
Sau3AI GATC 3 cut(s) 570, 636, 1096
ScaI AGTACT 1 cut(s) 803
SchI GAGTC 1 cut(s) 218
ScrFI CCNGG 5 cut(s) 413, 529, 547, 583, 870
SduI GDGCHC 2 cut(s) 291, 465
SfaNI GCATC 3 cut(s) 637, 850, 1250
Sfr274I CTCGAG 1 cut(s) 296
SfuI TTCGAA 1 cut(s) 534
SlaI CTCGAG 1 cut(s) 296
SmlI CTYRAG 2 cut(s) 284, 296
SmoI CTYRAG 2 cut(s) 284, 296
Sse9I AATT 8 cut(s) 407, 472, 556, 660, 760, 949, 1212, 1225
SspMI CTAG 2 cut(s) 1230, 1258
StyD4I CCNGG 5 cut(s) 411, 527, 545, 581, 868
TaaI ACNGT 2 cut(s) 427, 905
TaiI ACGT 1 cut(s) 656
TaqI TCGA 8 cut(s) 264, 297, 534, 573, 656, 875, 1106, 1145
TasI AATT 8 cut(s) 407, 472, 556, 660, 760, 949, 1212, 1225
TatI WGTACW 1 cut(s) 801
TauI GCSGC 2 cut(s) 140, 306
TfiI GAWTC 6 cut(s) 5, 435, 796, 973, 1208, 1250
TseI GCWGC 3 cut(s) 550, 561, 629
TspDTI ATGAA 2 cut(s) 1001, 1016
TspGWI ACGGA 3 cut(s) 25, 356, 825
Tth111I GACNNNGTC 1 cut(s) 311
XapI RAATTY 2 cut(s) 660, 760
XhoI CTCGAG 1 cut(s) 296
XspI CTAG 2 cut(s) 1230, 1258
ZrmI AGTACT 1 cut(s) 803
Zsp2I ATGCAT 1 cut(s) 1180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.