Rroxscaffold_7G00209110
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0009649
Physical Location & Seq
Forward (+)
59371039 .. 59374654
3616 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00209110.1

Sequence Viewer

Length: 1017 bp
ATGAATTCGAATAAAAGCTTGGGTCATGTGGTGTACTTGTTTGCTGGAAAGAAACTCCTCGTCCTCCTCGTCCTCTCCCCCAATTCCTCCACCGATTCCTCCTCCTCCTCCTCCCCCAACCATGCCTTCCCCGCCGTCCACCACTTCCTCTCCTCCCAAGAAATCGCCGCCACCCTCTCCCTCCTCTCCCTCTCCCGCAAACGCAAGCGGGCCCGTCTCGACTCCTCCGAGCCCACTCCCCCGGCCCAACTCGGCCTCCCTCTCCCTCGCACTCCCGACTCCTTCAAGACCTCTTTCCGAATGACCTCCTCAACCTTCGAGTGGCTCTGCTCCTTGCTCGAGCCCTTACTCGAGTGCAGGGACCCGATTGGCTCCCCGCTCAATCTCTCCGCCGACCTCCGCCTCGGAATCGGCCTGTTCCGCCTGGCCACCGGAGCCAATTACCCGGAGATATCAAGACAGTTCCGGGTTTCGGAGTCTGTGTCGAGATTTTGCTCTAAGCAATTGTGTCGTGTTCTCTGCACTAACTACCGGTTCTGGATTGAGTTTCCGAATAAGACTGAGCTTGAATCGGTGTCTTCGGGATTCAAAACCCACACCGGGTTGCCCAATTGCTGTGGTGTGATTGACTGTAGCCGGTTCAAGTTTGTGAGAAGTAATGGGGTTGATCAAGAAGAAGTTGCAGCCCAAATCATGGTGGATGCTAATTCCAGAATTCTCAGCATTGTGGCTGGGTTTAGGGGGAATAAAAGTGATGCTAGGGTTTTGAAGAGTTCGACTTTGTATGCAGATATTGAGGGTGGGAAGTTACTGAATTCGGATCCGGTTAGTGTGAATGGGGTGCCGGTGAATCAGTACTTGGTTGGAGATGAAGGGTACCCTTTGCTTCCTTGGCTTATGGTGCCCTTTGTTGATGCAATGCCAGGGTCGGATGAGGAGCAATTCAATGCAGCACACAGTAGAATGCGGCTTTCGGGGCTTAGAGCTATTGATAGTTTGAAGAATTGGGGGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

338

Amino Acids

37.18

Weight (kDa)

8.76

Isoelectric Point (pI)

48.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 227 - 336 1.3e-12 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 876
AccB1I GGYRCC 3 cut(s) 841, 876, 901
AccB7I CCANNNNNTGG 1 cut(s) 694
AccBSI CCGCTC 1 cut(s) 379
AciI CCGC 9 cut(s) 132, 168, 196, 208, 377, 390, 400, 421, 967
AclWI GGATC 2 cut(s) 815, 828
AcoI YGGCCR 1 cut(s) 426
AcsI RAATTY 3 cut(s) 4, 714, 814
AfaI GTAC 3 cut(s) 35, 857, 878
AfiI CCNNNNNNNGG 4 cut(s) 321, 472, 600, 694
AgeI ACCGGT 1 cut(s) 531
AgsI TTSAA 7 cut(s) 286, 569, 589, 643, 769, 946, 1000
AjnI CCWGG 2 cut(s) 423, 922
AjuI GAANNNNNNNTTGG 5 cut(s) 34, 110, 142, 842, 874
AluBI AGCT 3 cut(s) 18, 565, 986
AluI AGCT 3 cut(s) 18, 565, 986
Alw26I GTCTC 1 cut(s) 221
AlwI GGATC 2 cut(s) 815, 828
Ama87I CYCGRG 2 cut(s) 338, 350
AoxI GGCC 5 cut(s) 210, 243, 253, 412, 426
ApaI GGGCCC 1 cut(s) 214
ApeKI GCWGC 2 cut(s) 683, 950
ApoI RAATTY 3 cut(s) 4, 714, 814
AsiGI ACCGGT 1 cut(s) 531
Asp718I GGTACC 1 cut(s) 876
AspS9I GGNCC 4 cut(s) 210, 211, 244, 361
AsuC2I CCSGG 4 cut(s) 242, 446, 467, 601
AsuHPI GGTGA 1 cut(s) 859
AsuII TTCGAA 1 cut(s) 8
AvaI CYCGRG 2 cut(s) 338, 350
AvaII GGWCC 1 cut(s) 361
BaeGI GKGCMC 2 cut(s) 214, 906
BalI TGGCCA 1 cut(s) 428
BamHI GGATCC 1 cut(s) 820
BanI GGYRCC 3 cut(s) 841, 876, 901
BanII GRGCYC 3 cut(s) 214, 234, 345
BbsI GAAGAC 1 cut(s) 570
BbvI GCAGC 2 cut(s) 695, 962
BceAI ACGGC 1 cut(s) 119
BcgI CGANNNNNNTGC 2 cut(s) 491, 525
BciT130I CCWGG 2 cut(s) 425, 924
BclI TGATCA 1 cut(s) 667
BcnI CCSGG 4 cut(s) 242, 446, 467, 601
BcoDI GTCTC 1 cut(s) 221
BfaI CTAG 1 cut(s) 759
BfmI CTRYAG 1 cut(s) 631
BisI GCNGC 4 cut(s) 168, 684, 951, 968
BlsI GCNGC 4 cut(s) 169, 685, 952, 969
BmcAI AGTACT 1 cut(s) 857
Bme1390I CCNGG 6 cut(s) 242, 425, 446, 467, 601, 924
Bme18I GGWCC 1 cut(s) 361
BmeT110I CYCGRG 2 cut(s) 338, 350
BmgT120I GGNCC 4 cut(s) 210, 211, 244, 361
BmiI GGNNCC 9 cut(s) 212, 362, 363, 373, 436, 822, 843, 878, 903
BmrFI CCNGG 6 cut(s) 242, 425, 446, 467, 601, 924
BmsI GCATC 3 cut(s) 691, 745, 904
BpiI GAAGAC 1 cut(s) 570
Bpu14I TTCGAA 1 cut(s) 8
BpuMI CCSGG 4 cut(s) 242, 446, 467, 601
BsaJI CCNNGG 4 cut(s) 240, 403, 890, 923
BsaWI WCCGGW 3 cut(s) 431, 531, 823
BsaXI ACNNNNNCTCC 6 cut(s) 134, 164, 240, 270, 467, 497
Bsc4I CCNNNNNNNGG 4 cut(s) 321, 472, 600, 694
Bse118I RCCGGY 3 cut(s) 531, 636, 844
Bse3DI GCAATG 1 cut(s) 924
BseBI CCWGG 2 cut(s) 425, 924
BseDI CCNNGG 4 cut(s) 240, 403, 890, 923
BseGI GGATG 2 cut(s) 706, 937
BseLI CCNNNNNNNGG 4 cut(s) 321, 472, 600, 694
BseMI GCAATG 1 cut(s) 924
BseMII CTCAG 2 cut(s) 552, 733
BseSI GKGCMC 2 cut(s) 214, 906
BseXI GCAGC 2 cut(s) 695, 962
BseYI CCCAGC 1 cut(s) 731
BsgI GTGCAG 2 cut(s) 376, 505
BshFI GGCC 5 cut(s) 212, 245, 255, 414, 428
BshNI GGYRCC 3 cut(s) 841, 876, 901
BshTI ACCGGT 1 cut(s) 531
BsiHKCI CYCGRG 2 cut(s) 338, 350
BsiSI CCGG 9 cut(s) 242, 432, 446, 466, 532, 600, 637, 824, 845
BslFI GGGAC 1 cut(s) 374
BslI CCNNNNNNNGG 4 cut(s) 321, 472, 600, 694
BsmAI GTCTC 1 cut(s) 221
BsmBI CGTCTC 1 cut(s) 221
BsmFI GGGAC 1 cut(s) 374
BsmI GAATGC 1 cut(s) 969
BsnI GGCC 5 cut(s) 212, 245, 255, 414, 428
BsoBI CYCGRG 2 cut(s) 338, 350
Bsp119I TTCGAA 1 cut(s) 8
Bsp120I GGGCCC 1 cut(s) 210
Bsp1286I GDGCHC 4 cut(s) 214, 234, 345, 906
Bsp143I GATC 2 cut(s) 667, 820
BspACI CCGC 9 cut(s) 132, 168, 196, 208, 377, 390, 400, 421, 967
BspANI GGCC 5 cut(s) 212, 245, 255, 414, 428
BspCNI CTCAG 2 cut(s) 553, 732
BspLI GGNNCC 9 cut(s) 212, 362, 363, 373, 436, 822, 843, 878, 903
BspPI GGATC 2 cut(s) 815, 828
BspT104I TTCGAA 1 cut(s) 8
BspT107I GGYRCC 3 cut(s) 841, 876, 901
BsrBI CCGCTC 1 cut(s) 379
BsrDI GCAATG 1 cut(s) 924
BsrFI RCCGGY 3 cut(s) 531, 636, 844
BssAI RCCGGY 3 cut(s) 531, 636, 844
BssECI CCNNGG 4 cut(s) 240, 403, 890, 923
BssMI GATC 2 cut(s) 667, 820
BssT1I CCWWGG 1 cut(s) 890
Bst2UI CCWGG 2 cut(s) 425, 924
Bst4CI ACNGT 3 cut(s) 462, 632, 959
Bst6I CTCTTC 1 cut(s) 764
BstBI TTCGAA 1 cut(s) 8
BstC8I GCNNGC 2 cut(s) 206, 210
BstDEI CTNAG 4 cut(s) 498, 561, 719, 980
BstF5I GGATG 2 cut(s) 706, 937
BstKTI GATC 2 cut(s) 670, 823
BstMAI GTCTC 1 cut(s) 221
BstMBI GATC 2 cut(s) 667, 820
BstMWI GCNNNNNNNGC 6 cut(s) 131, 420, 434, 892, 901, 976
BstNI CCWGG 2 cut(s) 425, 924
BstSCI CCNGG 6 cut(s) 240, 423, 444, 465, 599, 922
BstSFI CTRYAG 1 cut(s) 631
BstSLI GKGCMC 2 cut(s) 214, 906
BstV1I GCAGC 2 cut(s) 695, 962
BstV2I GAAGAC 1 cut(s) 570
BstX2I RGATCY 1 cut(s) 820
BstYI RGATCY 1 cut(s) 820
BsuRI GGCC 5 cut(s) 212, 245, 255, 414, 428
BtsCI GGATG 2 cut(s) 706, 937
Cac8I GCNNGC 2 cut(s) 206, 210
Cfr10I RCCGGY 3 cut(s) 531, 636, 844
Cfr13I GGNCC 4 cut(s) 210, 211, 244, 361
Csp6I GTAC 3 cut(s) 34, 856, 877
CspAI ACCGGT 1 cut(s) 531
CspCI CAANNNNNGTGG 2 cut(s) 598, 633
CviAII CATG 3 cut(s) 26, 122, 694
CviQI GTAC 3 cut(s) 34, 856, 877
DdeI CTNAG 4 cut(s) 498, 561, 719, 980
DpnI GATC 2 cut(s) 669, 822
DpnII GATC 2 cut(s) 667, 820
EaeI YGGCCR 1 cut(s) 426
Eam1104I CTCTTC 1 cut(s) 764
EarI CTCTTC 1 cut(s) 764
EciI GGCGGA 3 cut(s) 379, 389, 410
Eco130I CCWWGG 1 cut(s) 890
Eco24I GRGCYC 3 cut(s) 214, 234, 345
Eco32I GATATC 1 cut(s) 453
Eco47I GGWCC 1 cut(s) 361
Eco88I CYCGRG 2 cut(s) 338, 350
EcoO109I RGGNCCY 1 cut(s) 361
EcoRI GAATTC 3 cut(s) 4, 714, 814
EcoRII CCWGG 2 cut(s) 423, 922
EcoRV GATATC 1 cut(s) 453
EcoT14I CCWWGG 1 cut(s) 890
EcoT38I GRGCYC 3 cut(s) 214, 234, 345
ErhI CCWWGG 1 cut(s) 890
Esp3I CGTCTC 1 cut(s) 221
FaeI CATG 3 cut(s) 29, 125, 697
FaiI YATR 5 cut(s) 27, 123, 695, 786, 899
FaqI GGGAC 1 cut(s) 374
FatI CATG 3 cut(s) 25, 121, 693
FauI CCCGC 4 cut(s) 139, 201, 203, 384
FbaI TGATCA 1 cut(s) 667
Fnu4HI GCNGC 4 cut(s) 168, 684, 951, 968
FokI GGATG 2 cut(s) 713, 944
FriOI GRGCYC 3 cut(s) 214, 234, 345
Fsp4HI GCNGC 4 cut(s) 168, 684, 951, 968
FspBI CTAG 1 cut(s) 759
GluI GCNGC 4 cut(s) 168, 684, 951, 968
GsaI CCCAGC 1 cut(s) 735
HaeIII GGCC 5 cut(s) 212, 245, 255, 414, 428
HapII CCGG 9 cut(s) 242, 432, 446, 466, 532, 600, 637, 824, 845
Hin1II CATG 3 cut(s) 29, 125, 697
HindIII AAGCTT 1 cut(s) 16
HinfI GANTC 8 cut(s) 95, 221, 278, 408, 476, 569, 585, 850
HpaII CCGG 9 cut(s) 242, 432, 446, 466, 532, 600, 637, 824, 845
HphI GGTGA 1 cut(s) 859
Hpy166II GTNNAC 2 cut(s) 34, 139
Hpy188I TCNGA 7 cut(s) 229, 299, 407, 475, 552, 820, 931
Hpy188III TCNNGA 9 cut(s) 218, 275, 286, 456, 486, 538, 582, 671, 711
Hpy8I GTNNAC 2 cut(s) 34, 139
HpyAV CCTTC 4 cut(s) 136, 292, 325, 866
HpyCH4III ACNGT 3 cut(s) 462, 632, 959
HpyCH4V TGCA 6 cut(s) 357, 522, 683, 788, 917, 950
HpyF10VI GCNNNNNNNGC 6 cut(s) 131, 420, 434, 892, 901, 976
HpyF3I CTNAG 4 cut(s) 498, 561, 719, 980
Hsp92II CATG 3 cut(s) 29, 125, 697
KflI GGGWCCC 1 cut(s) 361
KpnI GGTACC 1 cut(s) 880
Ksp22I TGATCA 1 cut(s) 667
Kzo9I GATC 2 cut(s) 667, 820
LmnI GCTCC 4 cut(s) 335, 377, 434, 937
Lsp1109I GCAGC 2 cut(s) 695, 962
LweI GCATC 3 cut(s) 691, 745, 904
MaeI CTAG 1 cut(s) 759
MaeIII GTNAC 1 cut(s) 807
MalI GATC 2 cut(s) 669, 822
MbiI CCGCTC 1 cut(s) 379
MboI GATC 2 cut(s) 667, 820
MboII GAAGA 4 cut(s) 570, 686, 781, 1012
MfeI CAATTG 2 cut(s) 503, 610
MflI RGATCY 1 cut(s) 820
MhlI GDGCHC 4 cut(s) 214, 234, 345, 906
MlsI TGGCCA 1 cut(s) 428
MluNI TGGCCA 1 cut(s) 428
MlyI GAGTC 3 cut(s) 215, 272, 485
MmeI TCCRAC 2 cut(s) 844, 909
Mox20I TGGCCA 1 cut(s) 428
MscI TGGCCA 1 cut(s) 428
Msp20I TGGCCA 1 cut(s) 428
MspI CCGG 9 cut(s) 242, 432, 446, 466, 532, 600, 637, 824, 845
MspR9I CCNGG 6 cut(s) 242, 425, 446, 467, 601, 924
MunI CAATTG 2 cut(s) 503, 610
Mva1269I GAATGC 1 cut(s) 969
MvaI CCWGG 2 cut(s) 425, 924
MwoI GCNNNNNNNGC 6 cut(s) 131, 420, 434, 892, 901, 976
NciI CCSGG 4 cut(s) 242, 446, 467, 601
NdeII GATC 2 cut(s) 667, 820
NlaIII CATG 3 cut(s) 29, 125, 697
NlaIV GGNNCC 9 cut(s) 212, 362, 363, 373, 436, 822, 843, 878, 903
NmeAIII GCCGAG 1 cut(s) 231
NspV TTCGAA 1 cut(s) 8
PaeR7I CTCGAG 2 cut(s) 338, 350
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 225
PctI GAATGC 1 cut(s) 969
PfeI GAWTC 5 cut(s) 95, 408, 569, 585, 850
PflMI CCANNNNNTGG 1 cut(s) 694
PinAI ACCGGT 1 cut(s) 531
PkrI GCNGC 4 cut(s) 169, 685, 952, 969
PleI GAGTC 3 cut(s) 215, 272, 484
PpsI GAGTC 3 cut(s) 215, 272, 484
PpuMI RGGWCCY 1 cut(s) 361
Psp5II RGGWCCY 1 cut(s) 361
Psp6I CCWGG 2 cut(s) 423, 922
PspFI CCCAGC 1 cut(s) 731
PspGI CCWGG 2 cut(s) 423, 922
PspN4I GGNNCC 9 cut(s) 212, 362, 363, 373, 436, 822, 843, 878, 903
PspOMI GGGCCC 1 cut(s) 210
PspPI GGNCC 4 cut(s) 210, 211, 244, 361
PspPPI RGGWCCY 1 cut(s) 361
PspXI VCTCGAGB 2 cut(s) 338, 350
PsuI RGATCY 1 cut(s) 820
RsaI GTAC 3 cut(s) 35, 857, 878
RsaNI GTAC 3 cut(s) 34, 856, 877
SatI GCNGC 4 cut(s) 168, 684, 951, 968
Sau3AI GATC 2 cut(s) 667, 820
Sau96I GGNCC 4 cut(s) 210, 211, 244, 361
ScaI AGTACT 1 cut(s) 857
SchI GAGTC 3 cut(s) 215, 272, 485
ScrFI CCNGG 6 cut(s) 242, 425, 446, 467, 601, 924
SduI GDGCHC 4 cut(s) 214, 234, 345, 906
SetI ASST 7 cut(s) 20, 293, 308, 317, 399, 567, 988
SfaNI GCATC 3 cut(s) 691, 745, 904
SfcI CTRYAG 1 cut(s) 631
Sfr274I CTCGAG 2 cut(s) 338, 350
SfuI TTCGAA 1 cut(s) 8
SinI GGWCC 1 cut(s) 361
SlaI CTCGAG 2 cut(s) 338, 350
SmlI CTYRAG 2 cut(s) 338, 350
SmoI CTYRAG 2 cut(s) 338, 350
SsiI CCGC 9 cut(s) 132, 168, 196, 208, 377, 390, 400, 421, 967
SspMI CTAG 1 cut(s) 759
StyD4I CCNGG 6 cut(s) 240, 423, 444, 465, 599, 922
StyI CCWWGG 1 cut(s) 890
TaaI ACNGT 3 cut(s) 462, 632, 959
TaqI TCGA 7 cut(s) 8, 219, 318, 339, 351, 485, 776
TatI WGTACW 2 cut(s) 33, 855
TauI GCSGC 2 cut(s) 170, 970
TfiI GAWTC 5 cut(s) 95, 408, 569, 585, 850
TseI GCWGC 2 cut(s) 683, 950
TspDTI ATGAA 2 cut(s) 17, 885
Van91I CCANNNNNTGG 1 cut(s) 694
VpaK11BI GGWCC 1 cut(s) 361
XapI RAATTY 3 cut(s) 4, 714, 814
XhoI CTCGAG 2 cut(s) 338, 350
XspI CTAG 1 cut(s) 759
ZrmI AGTACT 1 cut(s) 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.