RLG00000014740
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0009649
Physical Location & Seq
Forward (+)
57035990 .. 57037288
1299 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014740

Sequence Viewer

Length: 1299 bp
ATGGAATCTTCAAGCCTCGTAGCTTTACTCTCCTCCATCTCCCAACTCCTCCTCCTCCTCGTCCTCTCCCCCAATCCCTCCACCGATTCCTCCTCCTCCCCCAACCATGCCTTCCCCGCCGTCCACCACTTCCTCTCCTCCCAAGAAATCGCCGCCACCCTCTCCCTCCTCTCCCTCTCCCGCAAACGCAAGCGGGCCCGTCTCGACTCCTCCGAGCCCACTCCCCCGGCCCAACTCGGCCTCCCTCTCCCCCGCACCCCCGACTCCTTCAAGACCTCTTTCCGAATGACCTCCTCAACCTTCGAGTGGCTCTGCTCTTTACTCGAGCCCTTACTCGAGTGCAGGGACCCGATTGGCTCCCCGCTCAATCTCTCCGCCGACCTCCGCCTCGGAATCGGCCTGTTCCGCCTGGCCACCGGAGCCAATTACCCGGAGATATCGAGACAATTCCGGGTTTCGGAGTCTGTCTCCAGATTTTGCTCCAAGCAATTGTGTCGTGTTCTCTGCACTAACTACCGGTTCTGGATTGAGTTTCCGAATAAGACTGAGCTTGAATCGGTGTCTTCGGGTTTCGAAACCCACACCGGTTTGCCCAATTGCTGCGGTGTGATTGACTGTACCCGGTTCAAGTTTGTGAGAAGTAATGGGGTTGATCAAGAAGAAGTTGCAGCCCAAATCATGGTGGATGCTAATTCCAGAATTCTCAGCATTGTGGCTGGGTTCAGGGGGAATAAAAGTGATGCTAGGGTTTTGAAGAGTTCGACTCTGTATGCAGATATTGAGGGCGGGAAGTTACTGAATTCGGATCCGGTTAGTGTGAATGAGGTGCCGGTGAATCAGTACTTGGTTGGAGATGAAGGGTACCCTTTGCTTCCCTGGCTTATGGTGCCTTTTGTTGATGCAATACCGGGGTCGAATGAGGAGCAATTCAATGCAGCACACAGTAGAATGCGGCTTTCGGGGCTTAGAGCTATTGATAGTTTGAAGAATTGGGGGGTTTTGAGCAGACCGATTCGCGAGGAGATGAAGATGGCGGTTGCTTATATTGGTGCATGTTCTATACTACATAATGGTTTGTTGATGAGGGAGGACTATTCGGCAATGTCTGCTGGGTTGGATGATTGCTCTGTCTATGATCAGAGCTCTCATTATCATAGGGACAATTCAAGTTTGGAGGAGAGTTCAATTGAGAGGAAGGCTTCTGTTATAAGAAATGCATTGGCTACAAAAGCAAAAGAGTTTCAAGAATCAATATTTTCGACACATTCTAGCTCGTTGGTGTCATTGCAATCACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

433

Amino Acids

47.58

Weight (kDa)

6.18

Isoelectric Point (pI)

57.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 222 - 357 3.3e-18 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1208
Acc65I GGTACC 1 cut(s) 861
AccB1I GGYRCC 3 cut(s) 826, 861, 886
AccB7I CCANNNNNTGG 1 cut(s) 679
AccBSI CCGCTC 1 cut(s) 364
AccII CGCG 1 cut(s) 1017
AclWI GGATC 2 cut(s) 800, 813
AcoI YGGCCR 1 cut(s) 411
AcsI RAATTY 2 cut(s) 699, 799
AfaI GTAC 3 cut(s) 619, 842, 863
AfiI CCNNNNNNNGG 3 cut(s) 306, 457, 679
AgeI ACCGGT 2 cut(s) 516, 584
AjnI CCWGG 2 cut(s) 408, 875
AjuI GAANNNNNNNTTGG 4 cut(s) 95, 127, 827, 859
AluBI AGCT 5 cut(s) 23, 550, 971, 1143, 1272
AluI AGCT 5 cut(s) 23, 550, 971, 1143, 1272
Alw21I GWGCWC 1 cut(s) 1145
Alw26I GTCTC 3 cut(s) 206, 436, 472
AlwI GGATC 2 cut(s) 800, 813
Ama87I CYCGRG 2 cut(s) 323, 335
AoxI GGCC 5 cut(s) 195, 228, 238, 397, 411
ApaI GGGCCC 1 cut(s) 199
ApeKI GCWGC 3 cut(s) 600, 668, 935
ApoI RAATTY 2 cut(s) 699, 799
AsiGI ACCGGT 2 cut(s) 516, 584
Asp718I GGTACC 1 cut(s) 861
AspS9I GGNCC 4 cut(s) 195, 196, 229, 346
AsuC2I CCSGG 5 cut(s) 227, 431, 452, 622, 909
AsuHPI GGTGA 2 cut(s) 844, 1284
AsuII TTCGAA 1 cut(s) 573
AvaI CYCGRG 2 cut(s) 323, 335
AvaII GGWCC 1 cut(s) 346
BaeGI GKGCMC 1 cut(s) 199
BalI TGGCCA 1 cut(s) 413
BamHI GGATCC 1 cut(s) 805
BanI GGYRCC 3 cut(s) 826, 861, 886
BanII GRGCYC 4 cut(s) 199, 219, 330, 1145
BbsI GAAGAC 1 cut(s) 555
Bbv12I GWGCWC 1 cut(s) 1145
BbvI GCAGC 3 cut(s) 587, 680, 947
BccI CCATC 2 cut(s) 44, 1024
BceAI ACGGC 1 cut(s) 104
BcgI CGANNNNNNTGC 2 cut(s) 476, 510
BciT130I CCWGG 2 cut(s) 410, 877
BclI TGATCA 2 cut(s) 652, 1135
BcnI CCSGG 5 cut(s) 227, 431, 452, 622, 909
BcoDI GTCTC 3 cut(s) 206, 436, 472
BfaI CTAG 3 cut(s) 744, 1269, 1297
BisI GCNGC 5 cut(s) 153, 601, 669, 936, 953
BlsI GCNGC 5 cut(s) 154, 602, 670, 937, 954
BmcAI AGTACT 1 cut(s) 842
Bme1390I CCNGG 7 cut(s) 227, 410, 431, 452, 622, 877, 909
Bme18I GGWCC 1 cut(s) 346
BmeT110I CYCGRG 2 cut(s) 323, 335
BmgT120I GGNCC 4 cut(s) 195, 196, 229, 346
BmiI GGNNCC 9 cut(s) 197, 347, 348, 358, 421, 807, 828, 863, 888
BmrFI CCNGG 7 cut(s) 227, 410, 431, 452, 622, 877, 909
BmsI GCATC 3 cut(s) 676, 730, 889
BpiI GAAGAC 1 cut(s) 555
BplI GAGNNNNNCTC 4 cut(s) 452, 484, 748, 780
BpmI CTGGAG 1 cut(s) 454
Bpu14I TTCGAA 1 cut(s) 573
BpuMI CCSGG 5 cut(s) 227, 431, 452, 622, 909
BsaJI CCNNGG 4 cut(s) 225, 388, 875, 908
BsaWI WCCGGW 4 cut(s) 416, 516, 584, 808
BsaXI ACNNNNNCTCC 6 cut(s) 36, 66, 119, 149, 225, 255
Bsc4I CCNNNNNNNGG 3 cut(s) 306, 457, 679
Bse118I RCCGGY 3 cut(s) 516, 584, 829
Bse3DI GCAATG 2 cut(s) 1107, 1283
BseBI CCWGG 2 cut(s) 410, 877
BseDI CCNNGG 4 cut(s) 225, 388, 875, 908
BseGI GGATG 2 cut(s) 691, 1123
BseLI CCNNNNNNNGG 3 cut(s) 306, 457, 679
BseMI GCAATG 2 cut(s) 1107, 1283
BseMII CTCAG 2 cut(s) 537, 718
BseSI GKGCMC 1 cut(s) 199
BseXI GCAGC 3 cut(s) 587, 680, 947
BseYI CCCAGC 2 cut(s) 716, 1109
BsgI GTGCAG 2 cut(s) 361, 490
Bsh1236I CGCG 1 cut(s) 1017
BshFI GGCC 5 cut(s) 197, 230, 240, 399, 413
BshNI GGYRCC 3 cut(s) 826, 861, 886
BshTI ACCGGT 2 cut(s) 516, 584
BsiHKAI GWGCWC 1 cut(s) 1145
BsiHKCI CYCGRG 2 cut(s) 323, 335
BslFI GGGAC 2 cut(s) 359, 1172
BslI CCNNNNNNNGG 3 cut(s) 306, 457, 679
BsmAI GTCTC 3 cut(s) 206, 436, 472
BsmBI CGTCTC 1 cut(s) 206
BsmFI GGGAC 2 cut(s) 359, 1172
BsmI GAATGC 1 cut(s) 954
BsnI GGCC 5 cut(s) 197, 230, 240, 399, 413
BsoBI CYCGRG 2 cut(s) 323, 335
Bsp119I TTCGAA 1 cut(s) 573
Bsp120I GGGCCC 1 cut(s) 195
Bsp1286I GDGCHC 4 cut(s) 199, 219, 330, 1145
Bsp143I GATC 3 cut(s) 652, 805, 1135
Bsp68I TCGCGA 1 cut(s) 1017
BspANI GGCC 5 cut(s) 197, 230, 240, 399, 413
BspCNI CTCAG 2 cut(s) 538, 717
BspFNI CGCG 1 cut(s) 1017
BspLI GGNNCC 9 cut(s) 197, 347, 348, 358, 421, 807, 828, 863, 888
BspPI GGATC 2 cut(s) 800, 813
BspT104I TTCGAA 1 cut(s) 573
BspT107I GGYRCC 3 cut(s) 826, 861, 886
BsrBI CCGCTC 1 cut(s) 364
BsrDI GCAATG 2 cut(s) 1107, 1283
BsrFI RCCGGY 3 cut(s) 516, 584, 829
BssAI RCCGGY 3 cut(s) 516, 584, 829
BssECI CCNNGG 4 cut(s) 225, 388, 875, 908
BssMI GATC 3 cut(s) 652, 805, 1135
Bst2UI CCWGG 2 cut(s) 410, 877
Bst4CI ACNGT 2 cut(s) 617, 944
Bst6I CTCTTC 1 cut(s) 749
BstAPI GCANNNNNTGC 1 cut(s) 1106
BstBI TTCGAA 1 cut(s) 573
BstC8I GCNNGC 2 cut(s) 191, 195
BstDEI CTNAG 3 cut(s) 546, 704, 965
BstF5I GGATG 2 cut(s) 691, 1123
BstFNI CGCG 1 cut(s) 1017
BstKTI GATC 3 cut(s) 655, 808, 1138
BstMAI GTCTC 3 cut(s) 206, 436, 472
BstMBI GATC 3 cut(s) 652, 805, 1135
BstMWI GCNNNNNNNGC 8 cut(s) 116, 405, 419, 877, 886, 961, 1106, 1229
BstNI CCWGG 2 cut(s) 410, 877
BstNSI RCATGY 1 cut(s) 1056
BstSCI CCNGG 7 cut(s) 225, 408, 429, 450, 620, 875, 907
BstSLI GKGCMC 1 cut(s) 199
BstUI CGCG 1 cut(s) 1017
BstV1I GCAGC 3 cut(s) 587, 680, 947
BstV2I GAAGAC 1 cut(s) 555
BstX2I RGATCY 1 cut(s) 805
BstYI RGATCY 1 cut(s) 805
BsuRI GGCC 5 cut(s) 197, 230, 240, 399, 413
BtsCI GGATG 2 cut(s) 691, 1123
BtuMI TCGCGA 1 cut(s) 1017
Cac8I GCNNGC 2 cut(s) 191, 195
Cfr10I RCCGGY 3 cut(s) 516, 584, 829
Cfr13I GGNCC 4 cut(s) 195, 196, 229, 346
Csp6I GTAC 3 cut(s) 618, 841, 862
CspAI ACCGGT 2 cut(s) 516, 584
CviAII CATG 3 cut(s) 107, 679, 1053
CviQI GTAC 3 cut(s) 618, 841, 862
DdeI CTNAG 3 cut(s) 546, 704, 965
DpnI GATC 3 cut(s) 654, 807, 1137
DpnII GATC 3 cut(s) 652, 805, 1135
EaeI YGGCCR 1 cut(s) 411
Eam1104I CTCTTC 1 cut(s) 749
EarI CTCTTC 1 cut(s) 749
EciI GGCGGA 3 cut(s) 364, 374, 395
Ecl136II GAGCTC 1 cut(s) 1143
Eco24I GRGCYC 4 cut(s) 199, 219, 330, 1145
Eco32I GATATC 1 cut(s) 438
Eco47I GGWCC 1 cut(s) 346
Eco53kI GAGCTC 1 cut(s) 1143
Eco88I CYCGRG 2 cut(s) 323, 335
EcoICRI GAGCTC 1 cut(s) 1143
EcoO109I RGGNCCY 1 cut(s) 346
EcoRI GAATTC 2 cut(s) 699, 799
EcoRII CCWGG 2 cut(s) 408, 875
EcoRV GATATC 1 cut(s) 438
EcoT22I ATGCAT 1 cut(s) 1219
EcoT38I GRGCYC 4 cut(s) 199, 219, 330, 1145
Esp3I CGTCTC 1 cut(s) 206
FaeI CATG 3 cut(s) 110, 682, 1056
FaqI GGGAC 2 cut(s) 359, 1172
FatI CATG 3 cut(s) 106, 678, 1052
FauI CCCGC 6 cut(s) 124, 186, 188, 260, 369, 779
FbaI TGATCA 2 cut(s) 652, 1135
Fnu4HI GCNGC 5 cut(s) 153, 601, 669, 936, 953
FokI GGATG 2 cut(s) 698, 1130
FriOI GRGCYC 4 cut(s) 199, 219, 330, 1145
Fsp4HI GCNGC 5 cut(s) 153, 601, 669, 936, 953
FspBI CTAG 3 cut(s) 744, 1269, 1297
GluI GCNGC 5 cut(s) 153, 601, 669, 936, 953
GsaI CCCAGC 2 cut(s) 720, 1113
GsuI CTGGAG 1 cut(s) 454
HaeIII GGCC 5 cut(s) 197, 230, 240, 399, 413
Hin1II CATG 3 cut(s) 110, 682, 1056
HphI GGTGA 2 cut(s) 844, 1284
Hpy166II GTNNAC 1 cut(s) 124
Hpy188I TCNGA 7 cut(s) 214, 284, 392, 460, 537, 805, 1140
Hpy188III TCNNGA 9 cut(s) 203, 271, 441, 471, 523, 656, 696, 1016, 1244
Hpy8I GTNNAC 1 cut(s) 124
HpyAV CCTTC 5 cut(s) 121, 277, 310, 851, 1189
HpyCH4III ACNGT 2 cut(s) 617, 944
HpyCH4V TGCA 9 cut(s) 342, 507, 668, 773, 902, 935, 1052, 1217, 1288
HpyF10VI GCNNNNNNNGC 8 cut(s) 116, 405, 419, 877, 886, 961, 1106, 1229
HpyF3I CTNAG 3 cut(s) 546, 704, 965
Hsp92II CATG 3 cut(s) 110, 682, 1056
KflI GGGWCCC 1 cut(s) 346
KpnI GGTACC 1 cut(s) 865
Ksp22I TGATCA 2 cut(s) 652, 1135
Kzo9I GATC 3 cut(s) 652, 805, 1135
LmnI GCTCC 4 cut(s) 362, 419, 485, 922
Lsp1109I GCAGC 3 cut(s) 587, 680, 947
LweI GCATC 3 cut(s) 676, 730, 889
MaeI CTAG 3 cut(s) 744, 1269, 1297
MaeIII GTNAC 1 cut(s) 792
MalI GATC 3 cut(s) 654, 807, 1137
MbiI CCGCTC 1 cut(s) 364
MboI GATC 3 cut(s) 652, 805, 1135
MboII GAAGA 5 cut(s) 555, 671, 766, 997, 1039
MfeI CAATTG 3 cut(s) 488, 595, 1185
MflI RGATCY 1 cut(s) 805
MhlI GDGCHC 4 cut(s) 199, 219, 330, 1145
MlsI TGGCCA 1 cut(s) 413
MluNI TGGCCA 1 cut(s) 413
MlyI GAGTC 4 cut(s) 200, 257, 470, 757
MmeI TCCRAC 2 cut(s) 829, 1095
Mox20I TGGCCA 1 cut(s) 413
Mph1103I ATGCAT 1 cut(s) 1219
MscI TGGCCA 1 cut(s) 413
Msp20I TGGCCA 1 cut(s) 413
MspR9I CCNGG 7 cut(s) 227, 410, 431, 452, 622, 877, 909
MunI CAATTG 3 cut(s) 488, 595, 1185
Mva1269I GAATGC 1 cut(s) 954
MvaI CCWGG 2 cut(s) 410, 877
MvnI CGCG 1 cut(s) 1017
MwoI GCNNNNNNNGC 8 cut(s) 116, 405, 419, 877, 886, 961, 1106, 1229
NciI CCSGG 5 cut(s) 227, 431, 452, 622, 909
NdeII GATC 3 cut(s) 652, 805, 1135
NlaIII CATG 3 cut(s) 110, 682, 1056
NlaIV GGNNCC 9 cut(s) 197, 347, 348, 358, 421, 807, 828, 863, 888
NmeAIII GCCGAG 1 cut(s) 216
NruI TCGCGA 1 cut(s) 1017
NsiI ATGCAT 1 cut(s) 1219
NspI RCATGY 1 cut(s) 1056
NspV TTCGAA 1 cut(s) 573
PaeR7I CTCGAG 2 cut(s) 323, 335
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PctI GAATGC 1 cut(s) 954
PfeI GAWTC 7 cut(s) 5, 86, 393, 554, 835, 1012, 1247
PflMI CCANNNNNTGG 1 cut(s) 679
PinAI ACCGGT 2 cut(s) 516, 584
PkrI GCNGC 5 cut(s) 154, 602, 670, 937, 954
PleI GAGTC 4 cut(s) 200, 257, 469, 757
PpsI GAGTC 4 cut(s) 200, 257, 469, 757
PpuMI RGGWCCY 1 cut(s) 346
PsiI TTATAA 1 cut(s) 1208
Psp124BI GAGCTC 1 cut(s) 1145
Psp5II RGGWCCY 1 cut(s) 346
Psp6I CCWGG 2 cut(s) 408, 875
PspFI CCCAGC 2 cut(s) 716, 1109
PspGI CCWGG 2 cut(s) 408, 875
PspN4I GGNNCC 9 cut(s) 197, 347, 348, 358, 421, 807, 828, 863, 888
PspOMI GGGCCC 1 cut(s) 195
PspPI GGNCC 4 cut(s) 195, 196, 229, 346
PspPPI RGGWCCY 1 cut(s) 346
PspXI VCTCGAGB 2 cut(s) 323, 335
PsuI RGATCY 1 cut(s) 805
RruI TCGCGA 1 cut(s) 1017
RsaI GTAC 3 cut(s) 619, 842, 863
RsaNI GTAC 3 cut(s) 618, 841, 862
SacI GAGCTC 1 cut(s) 1145
SatI GCNGC 5 cut(s) 153, 601, 669, 936, 953
Sau3AI GATC 3 cut(s) 652, 805, 1135
Sau96I GGNCC 4 cut(s) 195, 196, 229, 346
ScaI AGTACT 1 cut(s) 842
SchI GAGTC 4 cut(s) 200, 257, 470, 757
ScrFI CCNGG 7 cut(s) 227, 410, 431, 452, 622, 877, 909
SduI GDGCHC 4 cut(s) 199, 219, 330, 1145
SfaNI GCATC 3 cut(s) 676, 730, 889
Sfr274I CTCGAG 2 cut(s) 323, 335
SfuI TTCGAA 1 cut(s) 573
SinI GGWCC 1 cut(s) 346
SlaI CTCGAG 2 cut(s) 323, 335
SmlI CTYRAG 2 cut(s) 323, 335
SmoI CTYRAG 2 cut(s) 323, 335
SspI AATATT 1 cut(s) 1254
SspMI CTAG 3 cut(s) 744, 1269, 1297
SstI GAGCTC 1 cut(s) 1145
StyD4I CCNGG 7 cut(s) 225, 408, 429, 450, 620, 875, 907
TaaI ACNGT 2 cut(s) 617, 944
TaqI TCGA 9 cut(s) 204, 303, 324, 336, 440, 573, 761, 914, 1259
TaqII GACCGA 1 cut(s) 1024
TatI WGTACW 1 cut(s) 840
TauI GCSGC 2 cut(s) 155, 955
TfiI GAWTC 7 cut(s) 5, 86, 393, 554, 835, 1012, 1247
TseI GCWGC 3 cut(s) 600, 668, 935
TspDTI ATGAA 2 cut(s) 870, 1040
Van91I CCANNNNNTGG 1 cut(s) 679
VpaK11BI GGWCC 1 cut(s) 346
XapI RAATTY 2 cut(s) 699, 799
XceI RCATGY 1 cut(s) 1056
XhoI CTCGAG 2 cut(s) 323, 335
XspI CTAG 3 cut(s) 744, 1269, 1297
ZrmI AGTACT 1 cut(s) 842
Zsp2I ATGCAT 1 cut(s) 1219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.