Rh6BG078300
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0009649
Physical Location & Seq
Reverse (-)
12046467 .. 12049582
3116 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG078300.1

Sequence Viewer

Length: 1275 bp
ATGGAATCTTCAACCCTCGTAGCTTTACTCTCCTCCATCTCCCAACTCCTCCTCCTCCTCGTCCTCTCCCCCCCCAATCCCTCCACCGATTCCTCCTCCCCCAACCATGCCTTCCCCGCCGTCCACCACTTCCTCTCCTCCCAAGAAATCGCCGCCACCCTCTCCCTCCTCTCCCTCTCCCGCAAACGCAAGCGGGCCCGTCTCGACTCCTCCGAGCCCACTCCCCCGGCCCAACTCGGCCTCCCTCGCACTCCCGACTCCTTCAAGACCTCTTTCCGAATGACCTCCTCAACCTTCGAGTGGCTCTGCTCTTTACTCGAGCCCTTACTCGAGTGCAGGGACCCGATTGGCTCCCCGCTCAATCTCTCCGCCGACCTCCGCCTCGGAATCGGCCTGTTCCGCCTGGCCACCGGAGCCAATTACCCGGAGATATCAAGACAGTTCCGGGTTTCCGAGTCTGTTTCGAGATTTTGCTCCAAGCAATTGTGTCGTGTTCTCTGTACTAACTACCGGTTCTGGATTGAGTTTCCGAATAAGACTGAGCTTGAATCGGTGTCTTCGGGTTTCGAAACCCACACCGGGTTGCCCAATTGCTGCGGTGTGATTGACTGTACCCGGTTCAAGTCTGTGAGAAGTAATGGGGTTGGTCAAGAAGAAGAAGTTGCAGCCCAAATCATGGTGGATGCTAATTCCAGAATTCTCAGCATTGTGGCTGGGTTCAGGGGGAATAAAAGTGATGCTAGGGTTTTGAAGAGTTCGACTCTGTATGCAGATATTGAGGGTGGGAAGTTACTGAATTCGGATCCGGTTAGTGTGAATGGGGTGCCGGTGAATCAGTACTTGGTTGGAGATGAAGGGTACCCTTTGCTTCCTTGGCTTATGGTGCCCTTTGTTGATGCAATGCCGGGGTCGGATGAGGAGCAATTCAATGCAGCACACACTAGAATGCGGCTTTCGGGGCTTAGGGCTATTGATAGTTTGAAGAATTGGGGGGTTTTGAGCATACCGATTCGCGAGGAGATGAAGATGGCTGTTGCTTATATTGGTGCTTGTTCTATTCTACATAATGGTTTGTTGATGAGGGAGGACTATTCGGCAATGTCTGCTGGGTTGGATGATTACTCTGTCTATGATCAGAGCTCTCAGTATCATAGGGACAATTCGAGTTTGGAGGAGAGTTCAATTGAGAGGAAGGCTTCTGTTATAAGAAATGCATTGGCTACTAAAGCAAAAGAGTTTCAAGAATCAATATTTTCGACACATTCTAGCTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

424

Amino Acids

46.65

Weight (kDa)

5.97

Isoelectric Point (pI)

54.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 221 - 356 8.4e-18 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1205
Acc65I GGTACC 1 cut(s) 858
AccB1I GGYRCC 3 cut(s) 823, 858, 883
AccB7I CCANNNNNTGG 1 cut(s) 676
AccBSI CCGCTC 1 cut(s) 358
AccII CGCG 1 cut(s) 1014
AclWI GGATC 2 cut(s) 797, 810
AcoI YGGCCR 1 cut(s) 405
AcsI RAATTY 2 cut(s) 696, 796
AfaI GTAC 4 cut(s) 502, 613, 839, 860
AfiI CCNNNNNNNGG 3 cut(s) 300, 579, 676
AgeI ACCGGT 1 cut(s) 510
AgsI TTSAA 9 cut(s) 12, 265, 548, 622, 751, 928, 982, 1182, 1241
AjnI CCWGG 1 cut(s) 402
AjuI GAANNNNNNNTTGG 4 cut(s) 95, 127, 824, 856
AluBI AGCT 4 cut(s) 23, 544, 1140, 1269
AluI AGCT 4 cut(s) 23, 544, 1140, 1269
Alw21I GWGCWC 1 cut(s) 1142
Alw26I GTCTC 1 cut(s) 206
AlwI GGATC 2 cut(s) 797, 810
Ama87I CYCGRG 2 cut(s) 317, 329
AoxI GGCC 5 cut(s) 195, 228, 238, 391, 405
ApaI GGGCCC 1 cut(s) 199
ApeKI GCWGC 3 cut(s) 594, 665, 932
ApoI RAATTY 2 cut(s) 696, 796
AsiGI ACCGGT 1 cut(s) 510
Asp718I GGTACC 1 cut(s) 858
AspS9I GGNCC 4 cut(s) 195, 196, 229, 340
AsuC2I CCSGG 6 cut(s) 227, 425, 446, 580, 616, 906
AsuHPI GGTGA 1 cut(s) 841
AsuII TTCGAA 1 cut(s) 567
AvaI CYCGRG 2 cut(s) 317, 329
AvaII GGWCC 1 cut(s) 340
BaeGI GKGCMC 2 cut(s) 199, 888
BalI TGGCCA 1 cut(s) 407
BamHI GGATCC 1 cut(s) 802
BanI GGYRCC 3 cut(s) 823, 858, 883
BanII GRGCYC 4 cut(s) 199, 219, 324, 1142
BbsI GAAGAC 1 cut(s) 549
Bbv12I GWGCWC 1 cut(s) 1142
BbvI GCAGC 3 cut(s) 581, 677, 944
BccI CCATC 2 cut(s) 44, 1021
BceAI ACGGC 1 cut(s) 104
BcgI CGANNNNNNTGC 2 cut(s) 470, 504
BciT130I CCWGG 1 cut(s) 404
BclI TGATCA 1 cut(s) 1132
BcnI CCSGG 6 cut(s) 227, 425, 446, 580, 616, 906
BcoDI GTCTC 1 cut(s) 206
BfaI CTAG 3 cut(s) 741, 942, 1266
BisI GCNGC 5 cut(s) 153, 595, 666, 933, 950
BlsI GCNGC 5 cut(s) 154, 596, 667, 934, 951
BmcAI AGTACT 1 cut(s) 839
Bme1390I CCNGG 7 cut(s) 227, 404, 425, 446, 580, 616, 906
Bme18I GGWCC 1 cut(s) 340
BmeT110I CYCGRG 2 cut(s) 317, 329
BmgT120I GGNCC 4 cut(s) 195, 196, 229, 340
BmiI GGNNCC 9 cut(s) 197, 341, 342, 352, 415, 804, 825, 860, 885
BmrFI CCNGG 7 cut(s) 227, 404, 425, 446, 580, 616, 906
BmsI GCATC 3 cut(s) 673, 727, 886
BpiI GAAGAC 1 cut(s) 549
BplI GAGNNNNNCTC 2 cut(s) 745, 777
Bpu10I CCTNAGC 1 cut(s) 962
Bpu14I TTCGAA 1 cut(s) 567
BpuMI CCSGG 6 cut(s) 227, 425, 446, 580, 616, 906
BsaJI CCNNGG 4 cut(s) 225, 382, 872, 905
BsaWI WCCGGW 3 cut(s) 410, 510, 805
BsaXI ACNNNNNCTCC 6 cut(s) 36, 66, 119, 149, 225, 255
Bsc4I CCNNNNNNNGG 3 cut(s) 300, 579, 676
Bse118I RCCGGY 2 cut(s) 510, 826
Bse3DI GCAATG 2 cut(s) 906, 1104
BseBI CCWGG 1 cut(s) 404
BseDI CCNNGG 4 cut(s) 225, 382, 872, 905
BseGI GGATG 3 cut(s) 688, 919, 1120
BseLI CCNNNNNNNGG 3 cut(s) 300, 579, 676
BseMI GCAATG 2 cut(s) 906, 1104
BseMII CTCAG 3 cut(s) 531, 715, 1157
BseSI GKGCMC 2 cut(s) 199, 888
BseXI GCAGC 3 cut(s) 581, 677, 944
BseYI CCCAGC 2 cut(s) 713, 1106
BsgI GTGCAG 1 cut(s) 355
Bsh1236I CGCG 1 cut(s) 1014
BshFI GGCC 5 cut(s) 197, 230, 240, 393, 407
BshNI GGYRCC 3 cut(s) 823, 858, 883
BshTI ACCGGT 1 cut(s) 510
BsiHKAI GWGCWC 1 cut(s) 1142
BsiHKCI CYCGRG 2 cut(s) 317, 329
BslFI GGGAC 2 cut(s) 353, 1169
BslI CCNNNNNNNGG 3 cut(s) 300, 579, 676
BsmAI GTCTC 1 cut(s) 206
BsmBI CGTCTC 1 cut(s) 206
BsmFI GGGAC 2 cut(s) 353, 1169
BsmI GAATGC 1 cut(s) 951
BsnI GGCC 5 cut(s) 197, 230, 240, 393, 407
BsoBI CYCGRG 2 cut(s) 317, 329
Bsp119I TTCGAA 1 cut(s) 567
Bsp120I GGGCCC 1 cut(s) 195
Bsp1286I GDGCHC 5 cut(s) 199, 219, 324, 888, 1142
Bsp143I GATC 2 cut(s) 802, 1132
Bsp68I TCGCGA 1 cut(s) 1014
BspANI GGCC 5 cut(s) 197, 230, 240, 393, 407
BspCNI CTCAG 3 cut(s) 532, 714, 1156
BspFNI CGCG 1 cut(s) 1014
BspLI GGNNCC 9 cut(s) 197, 341, 342, 352, 415, 804, 825, 860, 885
BspPI GGATC 2 cut(s) 797, 810
BspT104I TTCGAA 1 cut(s) 567
BspT107I GGYRCC 3 cut(s) 823, 858, 883
BsrBI CCGCTC 1 cut(s) 358
BsrDI GCAATG 2 cut(s) 906, 1104
BsrFI RCCGGY 2 cut(s) 510, 826
BssAI RCCGGY 2 cut(s) 510, 826
BssECI CCNNGG 4 cut(s) 225, 382, 872, 905
BssMI GATC 2 cut(s) 802, 1132
BssT1I CCWWGG 1 cut(s) 872
Bst2UI CCWGG 1 cut(s) 404
Bst4CI ACNGT 2 cut(s) 441, 611
Bst6I CTCTTC 1 cut(s) 746
BstAPI GCANNNNNTGC 1 cut(s) 1103
BstBI TTCGAA 1 cut(s) 567
BstC8I GCNNGC 2 cut(s) 191, 195
BstDEI CTNAG 4 cut(s) 540, 701, 962, 1143
BstF5I GGATG 3 cut(s) 688, 919, 1120
BstFNI CGCG 1 cut(s) 1014
BstKTI GATC 2 cut(s) 805, 1135
BstMAI GTCTC 1 cut(s) 206
BstMBI GATC 2 cut(s) 802, 1132
BstMWI GCNNNNNNNGC 9 cut(s) 116, 246, 399, 413, 874, 883, 958, 1103, 1226
BstNI CCWGG 1 cut(s) 404
BstSCI CCNGG 7 cut(s) 225, 402, 423, 444, 578, 614, 904
BstSLI GKGCMC 2 cut(s) 199, 888
BstUI CGCG 1 cut(s) 1014
BstV1I GCAGC 3 cut(s) 581, 677, 944
BstV2I GAAGAC 1 cut(s) 549
BstX2I RGATCY 1 cut(s) 802
BstYI RGATCY 1 cut(s) 802
BsuRI GGCC 5 cut(s) 197, 230, 240, 393, 407
BtsCI GGATG 3 cut(s) 688, 919, 1120
BtuMI TCGCGA 1 cut(s) 1014
Cac8I GCNNGC 2 cut(s) 191, 195
Cfr10I RCCGGY 2 cut(s) 510, 826
Cfr13I GGNCC 4 cut(s) 195, 196, 229, 340
Csp6I GTAC 4 cut(s) 501, 612, 838, 859
CspAI ACCGGT 1 cut(s) 510
CviAII CATG 2 cut(s) 107, 676
CviQI GTAC 4 cut(s) 501, 612, 838, 859
DdeI CTNAG 4 cut(s) 540, 701, 962, 1143
DpnI GATC 2 cut(s) 804, 1134
DpnII GATC 2 cut(s) 802, 1132
EaeI YGGCCR 1 cut(s) 405
Eam1104I CTCTTC 1 cut(s) 746
EarI CTCTTC 1 cut(s) 746
EciI GGCGGA 3 cut(s) 358, 368, 389
Ecl136II GAGCTC 1 cut(s) 1140
Eco130I CCWWGG 1 cut(s) 872
Eco24I GRGCYC 4 cut(s) 199, 219, 324, 1142
Eco32I GATATC 1 cut(s) 432
Eco47I GGWCC 1 cut(s) 340
Eco53kI GAGCTC 1 cut(s) 1140
Eco88I CYCGRG 2 cut(s) 317, 329
EcoICRI GAGCTC 1 cut(s) 1140
EcoO109I RGGNCCY 1 cut(s) 340
EcoRI GAATTC 2 cut(s) 696, 796
EcoRII CCWGG 1 cut(s) 402
EcoRV GATATC 1 cut(s) 432
EcoT14I CCWWGG 1 cut(s) 872
EcoT22I ATGCAT 1 cut(s) 1216
EcoT38I GRGCYC 4 cut(s) 199, 219, 324, 1142
ErhI CCWWGG 1 cut(s) 872
Esp3I CGTCTC 1 cut(s) 206
FaeI CATG 2 cut(s) 110, 679
FaqI GGGAC 2 cut(s) 353, 1169
FatI CATG 2 cut(s) 106, 675
FauI CCCGC 4 cut(s) 124, 186, 188, 363
FbaI TGATCA 1 cut(s) 1132
Fnu4HI GCNGC 5 cut(s) 153, 595, 666, 933, 950
FokI GGATG 3 cut(s) 695, 926, 1127
FriOI GRGCYC 4 cut(s) 199, 219, 324, 1142
Fsp4HI GCNGC 5 cut(s) 153, 595, 666, 933, 950
FspBI CTAG 3 cut(s) 741, 942, 1266
GluI GCNGC 5 cut(s) 153, 595, 666, 933, 950
GsaI CCCAGC 2 cut(s) 717, 1110
HaeIII GGCC 5 cut(s) 197, 230, 240, 393, 407
Hin1II CATG 2 cut(s) 110, 679
HphI GGTGA 1 cut(s) 841
Hpy166II GTNNAC 1 cut(s) 124
Hpy188I TCNGA 8 cut(s) 214, 278, 386, 454, 531, 802, 913, 1137
Hpy8I GTNNAC 1 cut(s) 124
HpyAV CCTTC 5 cut(s) 121, 271, 304, 848, 1186
HpyCH4III ACNGT 2 cut(s) 441, 611
HpyCH4V TGCA 6 cut(s) 336, 665, 770, 899, 932, 1214
HpyF10VI GCNNNNNNNGC 9 cut(s) 116, 246, 399, 413, 874, 883, 958, 1103, 1226
HpyF3I CTNAG 4 cut(s) 540, 701, 962, 1143
Hsp92II CATG 2 cut(s) 110, 679
KflI GGGWCCC 1 cut(s) 340
KpnI GGTACC 1 cut(s) 862
Ksp22I TGATCA 1 cut(s) 1132
Kzo9I GATC 2 cut(s) 802, 1132
LmnI GCTCC 4 cut(s) 356, 413, 479, 919
Lsp1109I GCAGC 3 cut(s) 581, 677, 944
LweI GCATC 3 cut(s) 673, 727, 886
MaeI CTAG 3 cut(s) 741, 942, 1266
MaeIII GTNAC 1 cut(s) 789
MalI GATC 2 cut(s) 804, 1134
MbiI CCGCTC 1 cut(s) 358
MboI GATC 2 cut(s) 802, 1132
MboII GAAGA 6 cut(s) 549, 665, 668, 763, 994, 1036
MfeI CAATTG 3 cut(s) 482, 589, 1182
MflI RGATCY 1 cut(s) 802
MhlI GDGCHC 5 cut(s) 199, 219, 324, 888, 1142
MlsI TGGCCA 1 cut(s) 407
MluNI TGGCCA 1 cut(s) 407
MlyI GAGTC 4 cut(s) 200, 251, 464, 754
MmeI TCCRAC 3 cut(s) 826, 891, 1092
Mox20I TGGCCA 1 cut(s) 407
Mph1103I ATGCAT 1 cut(s) 1216
MscI TGGCCA 1 cut(s) 407
MslI CAYNNNNRTG 1 cut(s) 944
Msp20I TGGCCA 1 cut(s) 407
MspR9I CCNGG 7 cut(s) 227, 404, 425, 446, 580, 616, 906
MunI CAATTG 3 cut(s) 482, 589, 1182
Mva1269I GAATGC 1 cut(s) 951
MvaI CCWGG 1 cut(s) 404
MvnI CGCG 1 cut(s) 1014
MwoI GCNNNNNNNGC 9 cut(s) 116, 246, 399, 413, 874, 883, 958, 1103, 1226
NciI CCSGG 6 cut(s) 227, 425, 446, 580, 616, 906
NdeII GATC 2 cut(s) 802, 1132
NlaIII CATG 2 cut(s) 110, 679
NlaIV GGNNCC 9 cut(s) 197, 341, 342, 352, 415, 804, 825, 860, 885
NmeAIII GCCGAG 1 cut(s) 216
NruI TCGCGA 1 cut(s) 1014
NsiI ATGCAT 1 cut(s) 1216
NspV TTCGAA 1 cut(s) 567
PaeR7I CTCGAG 2 cut(s) 317, 329
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PctI GAATGC 1 cut(s) 951
PfeI GAWTC 7 cut(s) 5, 89, 387, 548, 832, 1009, 1244
PflMI CCANNNNNTGG 1 cut(s) 676
PinAI ACCGGT 1 cut(s) 510
PkrI GCNGC 5 cut(s) 154, 596, 667, 934, 951
PleI GAGTC 4 cut(s) 200, 251, 463, 754
PpsI GAGTC 4 cut(s) 200, 251, 463, 754
PpuMI RGGWCCY 1 cut(s) 340
PsiI TTATAA 1 cut(s) 1205
Psp124BI GAGCTC 1 cut(s) 1142
Psp5II RGGWCCY 1 cut(s) 340
Psp6I CCWGG 1 cut(s) 402
PspFI CCCAGC 2 cut(s) 713, 1106
PspGI CCWGG 1 cut(s) 402
PspN4I GGNNCC 9 cut(s) 197, 341, 342, 352, 415, 804, 825, 860, 885
PspOMI GGGCCC 1 cut(s) 195
PspPI GGNCC 4 cut(s) 195, 196, 229, 340
PspPPI RGGWCCY 1 cut(s) 340
PspXI VCTCGAGB 2 cut(s) 317, 329
PsuI RGATCY 1 cut(s) 802
RruI TCGCGA 1 cut(s) 1014
RsaI GTAC 4 cut(s) 502, 613, 839, 860
RsaNI GTAC 4 cut(s) 501, 612, 838, 859
RseI CAYNNNNRTG 1 cut(s) 944
SacI GAGCTC 1 cut(s) 1142
SatI GCNGC 5 cut(s) 153, 595, 666, 933, 950
Sau3AI GATC 2 cut(s) 802, 1132
Sau96I GGNCC 4 cut(s) 195, 196, 229, 340
ScaI AGTACT 1 cut(s) 839
SchI GAGTC 4 cut(s) 200, 251, 464, 754
ScrFI CCNGG 7 cut(s) 227, 404, 425, 446, 580, 616, 906
SduI GDGCHC 5 cut(s) 199, 219, 324, 888, 1142
SetI ASST 8 cut(s) 25, 272, 287, 296, 378, 546, 1142, 1271
SfaNI GCATC 3 cut(s) 673, 727, 886
Sfr274I CTCGAG 2 cut(s) 317, 329
SfuI TTCGAA 1 cut(s) 567
SinI GGWCC 1 cut(s) 340
SlaI CTCGAG 2 cut(s) 317, 329
SmiMI CAYNNNNRTG 1 cut(s) 944
SmlI CTYRAG 2 cut(s) 317, 329
SmoI CTYRAG 2 cut(s) 317, 329
SspI AATATT 1 cut(s) 1251
SspMI CTAG 3 cut(s) 741, 942, 1266
SstI GAGCTC 1 cut(s) 1142
StyD4I CCNGG 7 cut(s) 225, 402, 423, 444, 578, 614, 904
StyI CCWWGG 1 cut(s) 872
TaaI ACNGT 2 cut(s) 441, 611
TaqI TCGA 9 cut(s) 204, 297, 318, 330, 464, 567, 758, 1163, 1256
TatI WGTACW 2 cut(s) 500, 837
TauI GCSGC 2 cut(s) 155, 952
TfiI GAWTC 7 cut(s) 5, 89, 387, 548, 832, 1009, 1244
TseI GCWGC 3 cut(s) 594, 665, 932
TspDTI ATGAA 2 cut(s) 867, 1037
Van91I CCANNNNNTGG 1 cut(s) 676
VpaK11BI GGWCC 1 cut(s) 340
XapI RAATTY 2 cut(s) 696, 796
XhoI CTCGAG 2 cut(s) 317, 329
XspI CTAG 3 cut(s) 741, 942, 1266
ZrmI AGTACT 1 cut(s) 839
Zsp2I ATGCAT 1 cut(s) 1216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.