Rh6CG072900
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0009649
Physical Location & Seq
Reverse (-)
7448065 .. 7451130
3066 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG072900.1

Sequence Viewer

Length: 1413 bp
ATGTACGATCACCAACAGCCAGGTGTTGCCTCTGTATTTCCTCTCCCAGATTTAACCCCCACGATTCCCAAGAAGCGAAAAAAAAAAATCCAAAATCAAAACCCCCAAAAAACACAAAAAAAAATGGAATCTTCAACCCTGGTAGCTTTACTCTCCTCCATCTCCCAACTCCTCCTCCTCCTCGTCCTCTCCCCCAATCCCTCCACCGATTCCTCCTCCCCCAACCATGCCTTCCCCGCCGTCCACCACTTCCTCTCCTCCCAAGAAATCGCCGCCACCCTCTCCCTCCTCTCCCTCTCCCGCAAACGCAAGCGGGCCCGTCTCGACTCCTCCGAGCCCACTCCCCCGGCCCAACTCGGCCTCCCTCGCACTCCCGACTCCTTCAAGACCTCTTTCCGAATGACCTCCTCAACCTTCGAGTGGCTCTGCTCTTTACTCGAGCCCTTACTCGAGTGCAGGGACCCGATTGGCTCCCCGCTCAATCTCTCCGCCGACCTCCGCCTCGGAATCGGCCTGTTCCGCCTGGCCACCGGAGCCAATTACCCGGAGATATCAAGACAGTTCCGGGTTTCCGAGTCTGTGTCCAGATTTTGCTCCAAGCAATTGTGTCGTGTTCTCTGCACTAACTACCGGTTCTGGATTGAGTTTCCGAATAAGACTGAGCTTGAATCGGTGTCTTCGGGTTTCGAAACCCACACCGGGTTGCCCAATTGCTGCGGTGTGATTGACTGTACCCGGTTCAAGTTTGTGAGGAGTAATGGGGTTGATCAAGAAGAAGTTGCAGCCCAGATCATGGTGGATGCTAATTCCAGAATTCTCAGCATTGTGGCTGGGTTCAGGGGGAATAAAAGTGATGCAAGGGTTTTGAAGAGTTCGACTCTGTATGCAGATATTGAGGGTGGGAAGTTACTGAATTCGGATCCGGTTAGTGTGAATGGGGTGCCGGTGAATCAGTACTTGGTTGGAGATGAAGGGTACCCTTTGCTTCCTTGGCTTATGGTGCCCTTTGTTGATGCAATGCCGGGGTCGGATGAGCAGCAATTCAATGCAGCACACAGTAGAATGCGGCTTTCGGGGCTTAGAGCTATTGATAGTTTGAAGAATTGGGGGGTTTTGAGCATACCGATTCGCGAGGAGATGAAGATGGCTGTTGCTTATATTGGTGCTTGTTCTATTCTACATAATGGTTTGTTGATGAGGGAGGACTATTCGGCAATGTCTGCTGGGTTGGATGATTACTCTGTCTATGATCAGAGCTCTCAGTATCATAGGGACAATTCGAGTTTGGAGGAGAGTTCAATTGAGAGGAAGGCTTCTGTTATAAGAAATGCATTGGCTACAAAAGCAAAAGAGTTTCAAGAATCAATATTTTCAACACATTCTAGCTCGTTGGTGTCATTGCAATCACCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

470

Amino Acids

51.99

Weight (kDa)

8.29

Isoelectric Point (pI)

55.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 260 - 395 2.2e-18 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1322
Acc65I GGTACC 1 cut(s) 975
AccB1I GGYRCC 3 cut(s) 940, 975, 1000
AccB7I CCANNNNNTGG 1 cut(s) 793
AccBSI CCGCTC 1 cut(s) 478
AccII CGCG 1 cut(s) 1131
AclWI GGATC 2 cut(s) 914, 927
AcoI YGGCCR 1 cut(s) 525
AcsI RAATTY 2 cut(s) 813, 913
AfaI GTAC 4 cut(s) 5, 733, 956, 977
AfiI CCNNNNNNNGG 3 cut(s) 420, 699, 793
AgeI ACCGGT 1 cut(s) 630
AjnI CCWGG 3 cut(s) 19, 138, 522
AjuI GAANNNNNNNTTGG 4 cut(s) 215, 247, 941, 973
AluBI AGCT 5 cut(s) 146, 664, 1085, 1257, 1386
AluI AGCT 5 cut(s) 146, 664, 1085, 1257, 1386
Alw21I GWGCWC 1 cut(s) 1259
Alw26I GTCTC 1 cut(s) 326
AlwI GGATC 2 cut(s) 914, 927
Ama87I CYCGRG 2 cut(s) 437, 449
AoxI GGCC 5 cut(s) 315, 348, 358, 511, 525
ApaI GGGCCC 1 cut(s) 319
ApeKI GCWGC 4 cut(s) 714, 782, 1036, 1049
ApoI RAATTY 2 cut(s) 813, 913
AsiGI ACCGGT 1 cut(s) 630
Asp718I GGTACC 1 cut(s) 975
AspS9I GGNCC 4 cut(s) 315, 316, 349, 460
AsuC2I CCSGG 6 cut(s) 347, 545, 566, 700, 736, 1023
AsuHPI GGTGA 2 cut(s) 958, 1398
AsuII TTCGAA 1 cut(s) 687
AvaI CYCGRG 2 cut(s) 437, 449
AvaII GGWCC 1 cut(s) 460
BaeGI GKGCMC 2 cut(s) 319, 1005
BalI TGGCCA 1 cut(s) 527
BamHI GGATCC 1 cut(s) 919
BanI GGYRCC 3 cut(s) 940, 975, 1000
BanII GRGCYC 4 cut(s) 319, 339, 444, 1259
BbsI GAAGAC 1 cut(s) 669
Bbv12I GWGCWC 1 cut(s) 1259
BbvI GCAGC 4 cut(s) 701, 794, 1048, 1061
BccI CCATC 2 cut(s) 167, 1138
BceAI ACGGC 1 cut(s) 224
BcgI CGANNNNNNTGC 2 cut(s) 590, 624
BciT130I CCWGG 3 cut(s) 21, 140, 524
BclI TGATCA 2 cut(s) 766, 1249
BcnI CCSGG 6 cut(s) 347, 545, 566, 700, 736, 1023
BcoDI GTCTC 1 cut(s) 326
BfaI CTAG 2 cut(s) 1383, 1411
BisI GCNGC 6 cut(s) 273, 715, 783, 1037, 1050, 1067
BlsI GCNGC 6 cut(s) 274, 716, 784, 1038, 1051, 1068
BmcAI AGTACT 1 cut(s) 956
Bme1390I CCNGG 9 cut(s) 21, 140, 347, 524, 545, 566, 700, 736, 1023
Bme18I GGWCC 1 cut(s) 460
BmeT110I CYCGRG 2 cut(s) 437, 449
BmgT120I GGNCC 4 cut(s) 315, 316, 349, 460
BmiI GGNNCC 9 cut(s) 317, 461, 462, 472, 535, 921, 942, 977, 1002
BmrFI CCNGG 9 cut(s) 21, 140, 347, 524, 545, 566, 700, 736, 1023
BmsI GCATC 3 cut(s) 790, 844, 1003
BpiI GAAGAC 1 cut(s) 669
BplI GAGNNNNNCTC 2 cut(s) 862, 894
Bpu14I TTCGAA 1 cut(s) 687
BpuMI CCSGG 6 cut(s) 347, 545, 566, 700, 736, 1023
BsaJI CCNNGG 5 cut(s) 138, 345, 502, 989, 1022
BsaWI WCCGGW 3 cut(s) 530, 630, 922
BsaXI ACNNNNNCTCC 6 cut(s) 159, 189, 239, 269, 345, 375
Bsc4I CCNNNNNNNGG 3 cut(s) 420, 699, 793
Bse118I RCCGGY 2 cut(s) 630, 943
Bse3DI GCAATG 3 cut(s) 1023, 1221, 1397
BseBI CCWGG 3 cut(s) 21, 140, 524
BseDI CCNNGG 5 cut(s) 138, 345, 502, 989, 1022
BseGI GGATG 3 cut(s) 805, 1036, 1237
BseLI CCNNNNNNNGG 3 cut(s) 420, 699, 793
BseMI GCAATG 3 cut(s) 1023, 1221, 1397
BseMII CTCAG 3 cut(s) 651, 832, 1274
BseSI GKGCMC 2 cut(s) 319, 1005
BseXI GCAGC 4 cut(s) 701, 794, 1048, 1061
BseYI CCCAGC 2 cut(s) 830, 1223
BsgI GTGCAG 2 cut(s) 475, 604
Bsh1236I CGCG 1 cut(s) 1131
BshFI GGCC 5 cut(s) 317, 350, 360, 513, 527
BshNI GGYRCC 3 cut(s) 940, 975, 1000
BshTI ACCGGT 1 cut(s) 630
BsiHKAI GWGCWC 1 cut(s) 1259
BsiHKCI CYCGRG 2 cut(s) 437, 449
BslFI GGGAC 2 cut(s) 473, 1286
BslI CCNNNNNNNGG 3 cut(s) 420, 699, 793
BsmAI GTCTC 1 cut(s) 326
BsmBI CGTCTC 1 cut(s) 326
BsmFI GGGAC 2 cut(s) 473, 1286
BsmI GAATGC 1 cut(s) 1068
BsnI GGCC 5 cut(s) 317, 350, 360, 513, 527
BsoBI CYCGRG 2 cut(s) 437, 449
Bsp119I TTCGAA 1 cut(s) 687
Bsp120I GGGCCC 1 cut(s) 315
Bsp1286I GDGCHC 5 cut(s) 319, 339, 444, 1005, 1259
Bsp143I GATC 5 cut(s) 7, 766, 789, 919, 1249
Bsp68I TCGCGA 1 cut(s) 1131
BspANI GGCC 5 cut(s) 317, 350, 360, 513, 527
BspCNI CTCAG 3 cut(s) 652, 831, 1273
BspFNI CGCG 1 cut(s) 1131
BspLI GGNNCC 9 cut(s) 317, 461, 462, 472, 535, 921, 942, 977, 1002
BspPI GGATC 2 cut(s) 914, 927
BspT104I TTCGAA 1 cut(s) 687
BspT107I GGYRCC 3 cut(s) 940, 975, 1000
BsrBI CCGCTC 1 cut(s) 478
BsrDI GCAATG 3 cut(s) 1023, 1221, 1397
BsrFI RCCGGY 2 cut(s) 630, 943
BssAI RCCGGY 2 cut(s) 630, 943
BssECI CCNNGG 5 cut(s) 138, 345, 502, 989, 1022
BssMI GATC 5 cut(s) 7, 766, 789, 919, 1249
BssT1I CCWWGG 1 cut(s) 989
Bst2UI CCWGG 3 cut(s) 21, 140, 524
Bst4CI ACNGT 3 cut(s) 561, 731, 1058
Bst6I CTCTTC 1 cut(s) 863
BstAPI GCANNNNNTGC 1 cut(s) 1220
BstBI TTCGAA 1 cut(s) 687
BstC8I GCNNGC 2 cut(s) 311, 315
BstDEI CTNAG 4 cut(s) 660, 818, 1079, 1260
BstF5I GGATG 3 cut(s) 805, 1036, 1237
BstFNI CGCG 1 cut(s) 1131
BstKTI GATC 5 cut(s) 10, 769, 792, 922, 1252
BstMAI GTCTC 1 cut(s) 326
BstMBI GATC 5 cut(s) 7, 766, 789, 919, 1249
BstMWI GCNNNNNNNGC 9 cut(s) 236, 366, 519, 533, 991, 1000, 1075, 1220, 1343
BstNI CCWGG 3 cut(s) 21, 140, 524
BstSCI CCNGG 9 cut(s) 19, 138, 345, 522, 543, 564, 698, 734, 1021
BstSLI GKGCMC 2 cut(s) 319, 1005
BstUI CGCG 1 cut(s) 1131
BstV1I GCAGC 4 cut(s) 701, 794, 1048, 1061
BstV2I GAAGAC 1 cut(s) 669
BstX2I RGATCY 1 cut(s) 919
BstYI RGATCY 1 cut(s) 919
BsuRI GGCC 5 cut(s) 317, 350, 360, 513, 527
BtsCI GGATG 3 cut(s) 805, 1036, 1237
BtuMI TCGCGA 1 cut(s) 1131
Cac8I GCNNGC 2 cut(s) 311, 315
Cfr10I RCCGGY 2 cut(s) 630, 943
Cfr13I GGNCC 4 cut(s) 315, 316, 349, 460
Csp6I GTAC 4 cut(s) 4, 732, 955, 976
CspAI ACCGGT 1 cut(s) 630
CviAII CATG 2 cut(s) 227, 793
CviQI GTAC 4 cut(s) 4, 732, 955, 976
DdeI CTNAG 4 cut(s) 660, 818, 1079, 1260
DpnI GATC 5 cut(s) 9, 768, 791, 921, 1251
DpnII GATC 5 cut(s) 7, 766, 789, 919, 1249
EaeI YGGCCR 1 cut(s) 525
Eam1104I CTCTTC 1 cut(s) 863
EarI CTCTTC 1 cut(s) 863
EciI GGCGGA 3 cut(s) 478, 488, 509
Ecl136II GAGCTC 1 cut(s) 1257
Eco130I CCWWGG 1 cut(s) 989
Eco24I GRGCYC 4 cut(s) 319, 339, 444, 1259
Eco32I GATATC 1 cut(s) 552
Eco47I GGWCC 1 cut(s) 460
Eco53kI GAGCTC 1 cut(s) 1257
Eco88I CYCGRG 2 cut(s) 437, 449
EcoICRI GAGCTC 1 cut(s) 1257
EcoO109I RGGNCCY 1 cut(s) 460
EcoRI GAATTC 2 cut(s) 813, 913
EcoRII CCWGG 3 cut(s) 19, 138, 522
EcoRV GATATC 1 cut(s) 552
EcoT14I CCWWGG 1 cut(s) 989
EcoT22I ATGCAT 1 cut(s) 1333
EcoT38I GRGCYC 4 cut(s) 319, 339, 444, 1259
ErhI CCWWGG 1 cut(s) 989
Esp3I CGTCTC 1 cut(s) 326
FaeI CATG 2 cut(s) 230, 796
FaqI GGGAC 2 cut(s) 473, 1286
FatI CATG 2 cut(s) 226, 792
FauI CCCGC 4 cut(s) 244, 306, 308, 483
FbaI TGATCA 2 cut(s) 766, 1249
Fnu4HI GCNGC 6 cut(s) 273, 715, 783, 1037, 1050, 1067
FokI GGATG 3 cut(s) 812, 1043, 1244
FriOI GRGCYC 4 cut(s) 319, 339, 444, 1259
Fsp4HI GCNGC 6 cut(s) 273, 715, 783, 1037, 1050, 1067
FspBI CTAG 2 cut(s) 1383, 1411
GluI GCNGC 6 cut(s) 273, 715, 783, 1037, 1050, 1067
GsaI CCCAGC 2 cut(s) 834, 1227
HaeIII GGCC 5 cut(s) 317, 350, 360, 513, 527
Hin1II CATG 2 cut(s) 230, 796
HphI GGTGA 2 cut(s) 958, 1398
Hpy166II GTNNAC 1 cut(s) 244
Hpy188I TCNGA 8 cut(s) 334, 398, 506, 574, 651, 919, 1030, 1254
Hpy8I GTNNAC 1 cut(s) 244
HpyAV CCTTC 5 cut(s) 241, 391, 424, 965, 1303
HpyCH4III ACNGT 3 cut(s) 561, 731, 1058
HpyCH4V TGCA 9 cut(s) 456, 621, 782, 857, 887, 1016, 1049, 1331, 1402
HpyF10VI GCNNNNNNNGC 9 cut(s) 236, 366, 519, 533, 991, 1000, 1075, 1220, 1343
HpyF3I CTNAG 4 cut(s) 660, 818, 1079, 1260
Hsp92II CATG 2 cut(s) 230, 796
KflI GGGWCCC 1 cut(s) 460
KpnI GGTACC 1 cut(s) 979
Ksp22I TGATCA 2 cut(s) 766, 1249
Kzo9I GATC 5 cut(s) 7, 766, 789, 919, 1249
LmnI GCTCC 3 cut(s) 476, 533, 599
Lsp1109I GCAGC 4 cut(s) 701, 794, 1048, 1061
LweI GCATC 3 cut(s) 790, 844, 1003
MaeI CTAG 2 cut(s) 1383, 1411
MaeIII GTNAC 1 cut(s) 906
MalI GATC 5 cut(s) 9, 768, 791, 921, 1251
MbiI CCGCTC 1 cut(s) 478
MboI GATC 5 cut(s) 7, 766, 789, 919, 1249
MboII GAAGA 6 cut(s) 123, 669, 785, 880, 1111, 1153
MfeI CAATTG 3 cut(s) 602, 709, 1299
MflI RGATCY 1 cut(s) 919
MhlI GDGCHC 5 cut(s) 319, 339, 444, 1005, 1259
MlsI TGGCCA 1 cut(s) 527
MluNI TGGCCA 1 cut(s) 527
MlyI GAGTC 4 cut(s) 320, 371, 584, 871
MmeI TCCRAC 3 cut(s) 943, 1008, 1209
Mox20I TGGCCA 1 cut(s) 527
Mph1103I ATGCAT 1 cut(s) 1333
MscI TGGCCA 1 cut(s) 527
MseI TTAA 1 cut(s) 53
Msp20I TGGCCA 1 cut(s) 527
MspR9I CCNGG 9 cut(s) 21, 140, 347, 524, 545, 566, 700, 736, 1023
MunI CAATTG 3 cut(s) 602, 709, 1299
Mva1269I GAATGC 1 cut(s) 1068
MvaI CCWGG 3 cut(s) 21, 140, 524
MvnI CGCG 1 cut(s) 1131
MwoI GCNNNNNNNGC 9 cut(s) 236, 366, 519, 533, 991, 1000, 1075, 1220, 1343
NciI CCSGG 6 cut(s) 347, 545, 566, 700, 736, 1023
NdeII GATC 5 cut(s) 7, 766, 789, 919, 1249
NlaIII CATG 2 cut(s) 230, 796
NlaIV GGNNCC 9 cut(s) 317, 461, 462, 472, 535, 921, 942, 977, 1002
NmeAIII GCCGAG 1 cut(s) 336
NruI TCGCGA 1 cut(s) 1131
NsiI ATGCAT 1 cut(s) 1333
NspV TTCGAA 1 cut(s) 687
PaeR7I CTCGAG 2 cut(s) 437, 449
PcsI WCGNNNNNNNCGW 1 cut(s) 330
PctI GAATGC 1 cut(s) 1068
PfeI GAWTC 8 cut(s) 64, 128, 209, 507, 668, 949, 1126, 1361
PflMI CCANNNNNTGG 1 cut(s) 793
PinAI ACCGGT 1 cut(s) 630
PkrI GCNGC 6 cut(s) 274, 716, 784, 1038, 1051, 1068
PleI GAGTC 4 cut(s) 320, 371, 583, 871
PpsI GAGTC 4 cut(s) 320, 371, 583, 871
PpuMI RGGWCCY 1 cut(s) 460
PsiI TTATAA 1 cut(s) 1322
Psp124BI GAGCTC 1 cut(s) 1259
Psp5II RGGWCCY 1 cut(s) 460
Psp6I CCWGG 3 cut(s) 19, 138, 522
PspFI CCCAGC 2 cut(s) 830, 1223
PspGI CCWGG 3 cut(s) 19, 138, 522
PspN4I GGNNCC 9 cut(s) 317, 461, 462, 472, 535, 921, 942, 977, 1002
PspOMI GGGCCC 1 cut(s) 315
PspPI GGNCC 4 cut(s) 315, 316, 349, 460
PspPPI RGGWCCY 1 cut(s) 460
PspXI VCTCGAGB 2 cut(s) 437, 449
PsuI RGATCY 1 cut(s) 919
RruI TCGCGA 1 cut(s) 1131
RsaI GTAC 4 cut(s) 5, 733, 956, 977
RsaNI GTAC 4 cut(s) 4, 732, 955, 976
SacI GAGCTC 1 cut(s) 1259
SaqAI TTAA 1 cut(s) 53
SatI GCNGC 6 cut(s) 273, 715, 783, 1037, 1050, 1067
Sau3AI GATC 5 cut(s) 7, 766, 789, 919, 1249
Sau96I GGNCC 4 cut(s) 315, 316, 349, 460
ScaI AGTACT 1 cut(s) 956
SchI GAGTC 4 cut(s) 320, 371, 584, 871
ScrFI CCNGG 9 cut(s) 21, 140, 347, 524, 545, 566, 700, 736, 1023
SduI GDGCHC 5 cut(s) 319, 339, 444, 1005, 1259
SfaNI GCATC 3 cut(s) 790, 844, 1003
Sfr274I CTCGAG 2 cut(s) 437, 449
SfuI TTCGAA 1 cut(s) 687
SinI GGWCC 1 cut(s) 460
SlaI CTCGAG 2 cut(s) 437, 449
SmlI CTYRAG 2 cut(s) 437, 449
SmoI CTYRAG 2 cut(s) 437, 449
SspI AATATT 1 cut(s) 1368
SspMI CTAG 2 cut(s) 1383, 1411
SstI GAGCTC 1 cut(s) 1259
StyD4I CCNGG 9 cut(s) 19, 138, 345, 522, 543, 564, 698, 734, 1021
StyI CCWWGG 1 cut(s) 989
TaaI ACNGT 3 cut(s) 561, 731, 1058
TaqI TCGA 7 cut(s) 324, 417, 438, 450, 687, 875, 1280
TatI WGTACW 1 cut(s) 954
TauI GCSGC 2 cut(s) 275, 1069
TfiI GAWTC 8 cut(s) 64, 128, 209, 507, 668, 949, 1126, 1361
Tru1I TTAA 1 cut(s) 53
Tru9I TTAA 1 cut(s) 53
TseI GCWGC 4 cut(s) 714, 782, 1036, 1049
TspDTI ATGAA 2 cut(s) 984, 1154
Van91I CCANNNNNTGG 1 cut(s) 793
VpaK11BI GGWCC 1 cut(s) 460
XapI RAATTY 2 cut(s) 813, 913
XhoI CTCGAG 2 cut(s) 437, 449
XspI CTAG 2 cut(s) 1383, 1411
ZrmI AGTACT 1 cut(s) 956
Zsp2I ATGCAT 1 cut(s) 1333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.