Rw6G007530
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0009649
Physical Location & Seq
Reverse (-)
12344807 .. 12346388
1582 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G007530.1

Sequence Viewer

Length: 1281 bp
ATGGAATCTTCAACCCTCGTAGCTTTACTCTCCTCCATCTCCCAACTCCTCCTCCTCCTCGTCCTCTCCCCCAATCCCTCCACCGATTCCTCCTCCCCCAACCATGCCTTCCCCGCCGTCCACCACTTCCTCTCCTCCCAAGAAATCGCCGCCACCCTCTCCCTCCTCTCCCTCTCCCGCAAACGCAAGCGGGCCCGTCTCGACTCCTCCGAGCCCACTCCCCCGGCCCAACTCGGCCTCCCTCTCCCTCTCCCTCGCACTCCCGACTCCTTCAAGACCTCTTTCCGAATGACCTCCTCAACCTTCGAGTGGCTCTGCTCTTTACTCGAGCCCTTACTCGAGTGCAGGGACCCGATTGGCTCCCCGCTCAATCTCTCCGCCGACCTCCGCCTCGGAATCGGCCTGTTCCGCCTGGCCACCGGAGCCAATTACCCGGAGATATCAAGACAGTTCCGGGTTTCCGAGTCTGTGTCCAGATTTTGCTCCAAGCAATTGTGTCGTGTTCTCTGTACTAACTACCGGTTCTGGATTGAGTTTCCGAATAAGACTGAGCTTGAATCGGTGTCTTCGGGTTTCGAAACCCACACCGGGTTGCCCAATTGCTGCGGTGTGATTGACTGTACCCGGTTCAAGTTTGTGAGAAGTAATGGGGTTGGTCAAGAAGAAGTTGCAGCCCAGATCATGGTGGATGCTAATTCCAGAATTCTCAGCATTGTGGCTGGGTTCAGGGGGAATAAAAGTGATGCCAGGGTTTTGAAGAGTTCGACTCTGCATGCAGATATTGAGGGTGGGAAGTTACTGAATTCGGATCCGGTGAGTGTGAATGGGGTGCCGGTGAATCAGTACTTGGTTGGAGATGAAGGGTACCCTTTGCTTCCTTGGCTTATGGTGCCCTTTGTTGATGCAATGCCGGGGTCGGATGAGGAGCAATTCAATGCAGCACACACTAGAATGCGGCTTTCAGGGCTTAGAGCTATTGATAGTTTGAAGAATTGGGGGGTTTTGAGCATACCGATTCGCGAGGAGATGAAGATGGCTGTTGCTTATATTGGTGCTTGTTCTATTCTACATAATGGTTTGTTGATGAGGGAGGACTATTCGGCAATGTCTGCTGGGTTGGATGATTACTCTGTCTATGATCAGAGCTCTCAGTATCATAGGGACAATTTGAGTTTGGAGGAGAGTTCAATTGAGAGGAAGGCTTCTGTTATAAGAAATGCACTGGCTACAAAAGCAAAAGAGTTTCAAGAATCAATATTTTCGACACATTCTAGCTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

426

Amino Acids

46.9

Weight (kDa)

6.17

Isoelectric Point (pI)

52.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 222 - 358 9.4e-18 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1211
Acc65I GGTACC 1 cut(s) 864
AccB1I GGYRCC 3 cut(s) 829, 864, 889
AccB7I CCANNNNNTGG 1 cut(s) 682
AccBSI CCGCTC 1 cut(s) 367
AccII CGCG 1 cut(s) 1020
AclWI GGATC 2 cut(s) 803, 816
AcoI YGGCCR 1 cut(s) 414
AcsI RAATTY 2 cut(s) 702, 802
AfaI GTAC 4 cut(s) 511, 622, 845, 866
AfiI CCNNNNNNNGG 3 cut(s) 309, 588, 682
AgeI ACCGGT 1 cut(s) 519
AgsI TTSAA 9 cut(s) 12, 274, 557, 631, 757, 934, 988, 1188, 1247
AjnI CCWGG 2 cut(s) 411, 746
AjuI GAANNNNNNNTTGG 4 cut(s) 92, 124, 830, 862
AluBI AGCT 5 cut(s) 23, 553, 974, 1146, 1275
AluI AGCT 5 cut(s) 23, 553, 974, 1146, 1275
Alw21I GWGCWC 1 cut(s) 1148
Alw26I GTCTC 1 cut(s) 203
AlwI GGATC 2 cut(s) 803, 816
Ama87I CYCGRG 2 cut(s) 326, 338
AoxI GGCC 5 cut(s) 192, 225, 235, 400, 414
ApaI GGGCCC 1 cut(s) 196
ApeKI GCWGC 3 cut(s) 603, 671, 938
ApoI RAATTY 2 cut(s) 702, 802
AsiGI ACCGGT 1 cut(s) 519
Asp718I GGTACC 1 cut(s) 864
AspS9I GGNCC 4 cut(s) 192, 193, 226, 349
AsuC2I CCSGG 6 cut(s) 224, 434, 455, 589, 625, 912
AsuHPI GGTGA 2 cut(s) 826, 847
AsuII TTCGAA 1 cut(s) 576
AvaI CYCGRG 2 cut(s) 326, 338
AvaII GGWCC 1 cut(s) 349
BaeGI GKGCMC 2 cut(s) 196, 894
BalI TGGCCA 1 cut(s) 416
BamHI GGATCC 1 cut(s) 808
BanI GGYRCC 3 cut(s) 829, 864, 889
BanII GRGCYC 4 cut(s) 196, 216, 333, 1148
BbsI GAAGAC 1 cut(s) 558
Bbv12I GWGCWC 1 cut(s) 1148
BbvI GCAGC 3 cut(s) 590, 683, 950
BccI CCATC 2 cut(s) 44, 1027
BceAI ACGGC 1 cut(s) 101
BcgI CGANNNNNNTGC 2 cut(s) 479, 513
BciT130I CCWGG 2 cut(s) 413, 748
BclI TGATCA 1 cut(s) 1138
BcnI CCSGG 6 cut(s) 224, 434, 455, 589, 625, 912
BcoDI GTCTC 1 cut(s) 203
BfaI CTAG 2 cut(s) 948, 1272
BisI GCNGC 5 cut(s) 150, 604, 672, 939, 956
BlsI GCNGC 5 cut(s) 151, 605, 673, 940, 957
BmcAI AGTACT 1 cut(s) 845
Bme1390I CCNGG 8 cut(s) 224, 413, 434, 455, 589, 625, 748, 912
Bme18I GGWCC 1 cut(s) 349
BmeT110I CYCGRG 2 cut(s) 326, 338
BmgT120I GGNCC 4 cut(s) 192, 193, 226, 349
BmiI GGNNCC 9 cut(s) 194, 350, 351, 361, 424, 810, 831, 866, 891
BmrFI CCNGG 8 cut(s) 224, 413, 434, 455, 589, 625, 748, 912
BmsI GCATC 3 cut(s) 679, 733, 892
BpiI GAAGAC 1 cut(s) 558
BplI GAGNNNNNCTC 2 cut(s) 751, 783
Bpu14I TTCGAA 1 cut(s) 576
BpuMI CCSGG 6 cut(s) 224, 434, 455, 589, 625, 912
BsaJI CCNNGG 5 cut(s) 222, 391, 747, 878, 911
BsaWI WCCGGW 3 cut(s) 419, 519, 811
BsaXI ACNNNNNCTCC 6 cut(s) 36, 66, 116, 146, 222, 252
Bsc4I CCNNNNNNNGG 3 cut(s) 309, 588, 682
Bse118I RCCGGY 2 cut(s) 519, 832
Bse1I ACTGG 1 cut(s) 1227
Bse3DI GCAATG 2 cut(s) 912, 1110
BseBI CCWGG 2 cut(s) 413, 748
BseDI CCNNGG 5 cut(s) 222, 391, 747, 878, 911
BseGI GGATG 3 cut(s) 694, 925, 1126
BseLI CCNNNNNNNGG 3 cut(s) 309, 588, 682
BseMI GCAATG 2 cut(s) 912, 1110
BseMII CTCAG 3 cut(s) 540, 721, 1163
BseNI ACTGG 1 cut(s) 1227
BseSI GKGCMC 2 cut(s) 196, 894
BseXI GCAGC 3 cut(s) 590, 683, 950
BseYI CCCAGC 2 cut(s) 719, 1112
BsgI GTGCAG 1 cut(s) 364
Bsh1236I CGCG 1 cut(s) 1020
BshFI GGCC 5 cut(s) 194, 227, 237, 402, 416
BshNI GGYRCC 3 cut(s) 829, 864, 889
BshTI ACCGGT 1 cut(s) 519
BsiHKAI GWGCWC 1 cut(s) 1148
BsiHKCI CYCGRG 2 cut(s) 326, 338
BslFI GGGAC 2 cut(s) 362, 1175
BslI CCNNNNNNNGG 3 cut(s) 309, 588, 682
BsmAI GTCTC 1 cut(s) 203
BsmBI CGTCTC 1 cut(s) 203
BsmFI GGGAC 2 cut(s) 362, 1175
BsmI GAATGC 1 cut(s) 957
BsnI GGCC 5 cut(s) 194, 227, 237, 402, 416
BsoBI CYCGRG 2 cut(s) 326, 338
Bsp119I TTCGAA 1 cut(s) 576
Bsp120I GGGCCC 1 cut(s) 192
Bsp1286I GDGCHC 5 cut(s) 196, 216, 333, 894, 1148
Bsp143I GATC 3 cut(s) 678, 808, 1138
Bsp68I TCGCGA 1 cut(s) 1020
BspANI GGCC 5 cut(s) 194, 227, 237, 402, 416
BspCNI CTCAG 3 cut(s) 541, 720, 1162
BspFNI CGCG 1 cut(s) 1020
BspLI GGNNCC 9 cut(s) 194, 350, 351, 361, 424, 810, 831, 866, 891
BspPI GGATC 2 cut(s) 803, 816
BspT104I TTCGAA 1 cut(s) 576
BspT107I GGYRCC 3 cut(s) 829, 864, 889
BsrBI CCGCTC 1 cut(s) 367
BsrDI GCAATG 2 cut(s) 912, 1110
BsrFI RCCGGY 2 cut(s) 519, 832
BsrI ACTGG 1 cut(s) 1227
BssAI RCCGGY 2 cut(s) 519, 832
BssECI CCNNGG 5 cut(s) 222, 391, 747, 878, 911
BssMI GATC 3 cut(s) 678, 808, 1138
BssT1I CCWWGG 1 cut(s) 878
Bst2UI CCWGG 2 cut(s) 413, 748
Bst4CI ACNGT 2 cut(s) 450, 620
Bst6I CTCTTC 1 cut(s) 752
BstAPI GCANNNNNTGC 1 cut(s) 1109
BstBI TTCGAA 1 cut(s) 576
BstC8I GCNNGC 3 cut(s) 188, 192, 774
BstDEI CTNAG 4 cut(s) 549, 707, 968, 1149
BstF5I GGATG 3 cut(s) 694, 925, 1126
BstFNI CGCG 1 cut(s) 1020
BstKTI GATC 3 cut(s) 681, 811, 1141
BstMAI GTCTC 1 cut(s) 203
BstMBI GATC 3 cut(s) 678, 808, 1138
BstMWI GCNNNNNNNGC 8 cut(s) 113, 408, 422, 880, 889, 964, 1109, 1232
BstNI CCWGG 2 cut(s) 413, 748
BstNSI RCATGY 1 cut(s) 776
BstSCI CCNGG 8 cut(s) 222, 411, 432, 453, 587, 623, 746, 910
BstSLI GKGCMC 2 cut(s) 196, 894
BstUI CGCG 1 cut(s) 1020
BstV1I GCAGC 3 cut(s) 590, 683, 950
BstV2I GAAGAC 1 cut(s) 558
BstX2I RGATCY 1 cut(s) 808
BstYI RGATCY 1 cut(s) 808
BsuRI GGCC 5 cut(s) 194, 227, 237, 402, 416
BtsCI GGATG 3 cut(s) 694, 925, 1126
BtsIMutI CAGTG 1 cut(s) 1220
BtuMI TCGCGA 1 cut(s) 1020
Cac8I GCNNGC 3 cut(s) 188, 192, 774
Cfr10I RCCGGY 2 cut(s) 519, 832
Cfr13I GGNCC 4 cut(s) 192, 193, 226, 349
Csp6I GTAC 4 cut(s) 510, 621, 844, 865
CspAI ACCGGT 1 cut(s) 519
CviAII CATG 3 cut(s) 104, 682, 773
CviQI GTAC 4 cut(s) 510, 621, 844, 865
DdeI CTNAG 4 cut(s) 549, 707, 968, 1149
DpnI GATC 3 cut(s) 680, 810, 1140
DpnII GATC 3 cut(s) 678, 808, 1138
EaeI YGGCCR 1 cut(s) 414
Eam1104I CTCTTC 1 cut(s) 752
EarI CTCTTC 1 cut(s) 752
EciI GGCGGA 3 cut(s) 367, 377, 398
Ecl136II GAGCTC 1 cut(s) 1146
Eco130I CCWWGG 1 cut(s) 878
Eco24I GRGCYC 4 cut(s) 196, 216, 333, 1148
Eco32I GATATC 1 cut(s) 441
Eco47I GGWCC 1 cut(s) 349
Eco53kI GAGCTC 1 cut(s) 1146
Eco88I CYCGRG 2 cut(s) 326, 338
EcoICRI GAGCTC 1 cut(s) 1146
EcoO109I RGGNCCY 1 cut(s) 349
EcoRI GAATTC 2 cut(s) 702, 802
EcoRII CCWGG 2 cut(s) 411, 746
EcoRV GATATC 1 cut(s) 441
EcoT14I CCWWGG 1 cut(s) 878
EcoT38I GRGCYC 4 cut(s) 196, 216, 333, 1148
ErhI CCWWGG 1 cut(s) 878
Esp3I CGTCTC 1 cut(s) 203
FaeI CATG 3 cut(s) 107, 685, 776
FaqI GGGAC 2 cut(s) 362, 1175
FatI CATG 3 cut(s) 103, 681, 772
FauI CCCGC 4 cut(s) 121, 183, 185, 372
FbaI TGATCA 1 cut(s) 1138
Fnu4HI GCNGC 5 cut(s) 150, 604, 672, 939, 956
FokI GGATG 3 cut(s) 701, 932, 1133
FriOI GRGCYC 4 cut(s) 196, 216, 333, 1148
Fsp4HI GCNGC 5 cut(s) 150, 604, 672, 939, 956
FspBI CTAG 2 cut(s) 948, 1272
GluI GCNGC 5 cut(s) 150, 604, 672, 939, 956
GsaI CCCAGC 2 cut(s) 723, 1116
HaeIII GGCC 5 cut(s) 194, 227, 237, 402, 416
Hin1II CATG 3 cut(s) 107, 685, 776
HphI GGTGA 2 cut(s) 826, 847
Hpy166II GTNNAC 1 cut(s) 121
Hpy188I TCNGA 8 cut(s) 211, 287, 395, 463, 540, 808, 919, 1143
Hpy8I GTNNAC 1 cut(s) 121
HpyAV CCTTC 5 cut(s) 118, 280, 313, 854, 1192
HpyCH4III ACNGT 2 cut(s) 450, 620
HpyCH4V TGCA 7 cut(s) 345, 671, 772, 776, 905, 938, 1220
HpyF10VI GCNNNNNNNGC 8 cut(s) 113, 408, 422, 880, 889, 964, 1109, 1232
HpyF3I CTNAG 4 cut(s) 549, 707, 968, 1149
Hsp92II CATG 3 cut(s) 107, 685, 776
KflI GGGWCCC 1 cut(s) 349
KpnI GGTACC 1 cut(s) 868
Ksp22I TGATCA 1 cut(s) 1138
Kzo9I GATC 3 cut(s) 678, 808, 1138
LmnI GCTCC 4 cut(s) 365, 422, 488, 925
Lsp1109I GCAGC 3 cut(s) 590, 683, 950
LweI GCATC 3 cut(s) 679, 733, 892
MaeI CTAG 2 cut(s) 948, 1272
MaeIII GTNAC 1 cut(s) 795
MalI GATC 3 cut(s) 680, 810, 1140
MbiI CCGCTC 1 cut(s) 367
MboI GATC 3 cut(s) 678, 808, 1138
MboII GAAGA 5 cut(s) 558, 674, 769, 1000, 1042
MfeI CAATTG 3 cut(s) 491, 598, 1188
MflI RGATCY 1 cut(s) 808
MhlI GDGCHC 5 cut(s) 196, 216, 333, 894, 1148
MlsI TGGCCA 1 cut(s) 416
MluNI TGGCCA 1 cut(s) 416
MlyI GAGTC 4 cut(s) 197, 260, 473, 760
MmeI TCCRAC 3 cut(s) 832, 897, 1098
Mox20I TGGCCA 1 cut(s) 416
MscI TGGCCA 1 cut(s) 416
MslI CAYNNNNRTG 1 cut(s) 950
Msp20I TGGCCA 1 cut(s) 416
MspR9I CCNGG 8 cut(s) 224, 413, 434, 455, 589, 625, 748, 912
MunI CAATTG 3 cut(s) 491, 598, 1188
Mva1269I GAATGC 1 cut(s) 957
MvaI CCWGG 2 cut(s) 413, 748
MvnI CGCG 1 cut(s) 1020
MwoI GCNNNNNNNGC 8 cut(s) 113, 408, 422, 880, 889, 964, 1109, 1232
NciI CCSGG 6 cut(s) 224, 434, 455, 589, 625, 912
NdeII GATC 3 cut(s) 678, 808, 1138
NlaIII CATG 3 cut(s) 107, 685, 776
NlaIV GGNNCC 9 cut(s) 194, 350, 351, 361, 424, 810, 831, 866, 891
NmeAIII GCCGAG 1 cut(s) 213
NruI TCGCGA 1 cut(s) 1020
NspI RCATGY 1 cut(s) 776
NspV TTCGAA 1 cut(s) 576
PaeI GCATGC 1 cut(s) 776
PaeR7I CTCGAG 2 cut(s) 326, 338
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PctI GAATGC 1 cut(s) 957
PfeI GAWTC 7 cut(s) 5, 86, 396, 557, 838, 1015, 1250
PflMI CCANNNNNTGG 1 cut(s) 682
PinAI ACCGGT 1 cut(s) 519
PkrI GCNGC 5 cut(s) 151, 605, 673, 940, 957
PleI GAGTC 4 cut(s) 197, 260, 472, 760
PpsI GAGTC 4 cut(s) 197, 260, 472, 760
PpuMI RGGWCCY 1 cut(s) 349
PsiI TTATAA 1 cut(s) 1211
Psp124BI GAGCTC 1 cut(s) 1148
Psp5II RGGWCCY 1 cut(s) 349
Psp6I CCWGG 2 cut(s) 411, 746
PspFI CCCAGC 2 cut(s) 719, 1112
PspGI CCWGG 2 cut(s) 411, 746
PspN4I GGNNCC 9 cut(s) 194, 350, 351, 361, 424, 810, 831, 866, 891
PspOMI GGGCCC 1 cut(s) 192
PspPI GGNCC 4 cut(s) 192, 193, 226, 349
PspPPI RGGWCCY 1 cut(s) 349
PspXI VCTCGAGB 2 cut(s) 326, 338
PsuI RGATCY 1 cut(s) 808
RruI TCGCGA 1 cut(s) 1020
RsaI GTAC 4 cut(s) 511, 622, 845, 866
RsaNI GTAC 4 cut(s) 510, 621, 844, 865
RseI CAYNNNNRTG 1 cut(s) 950
SacI GAGCTC 1 cut(s) 1148
SatI GCNGC 5 cut(s) 150, 604, 672, 939, 956
Sau3AI GATC 3 cut(s) 678, 808, 1138
Sau96I GGNCC 4 cut(s) 192, 193, 226, 349
ScaI AGTACT 1 cut(s) 845
SchI GAGTC 4 cut(s) 197, 260, 473, 760
ScrFI CCNGG 8 cut(s) 224, 413, 434, 455, 589, 625, 748, 912
SduI GDGCHC 5 cut(s) 196, 216, 333, 894, 1148
SetI ASST 9 cut(s) 25, 281, 296, 305, 387, 555, 976, 1148, 1277
SfaNI GCATC 3 cut(s) 679, 733, 892
Sfr274I CTCGAG 2 cut(s) 326, 338
SfuI TTCGAA 1 cut(s) 576
SinI GGWCC 1 cut(s) 349
SlaI CTCGAG 2 cut(s) 326, 338
SmiMI CAYNNNNRTG 1 cut(s) 950
SmlI CTYRAG 2 cut(s) 326, 338
SmoI CTYRAG 2 cut(s) 326, 338
SphI GCATGC 1 cut(s) 776
SspI AATATT 1 cut(s) 1257
SspMI CTAG 2 cut(s) 948, 1272
SstI GAGCTC 1 cut(s) 1148
StyD4I CCNGG 8 cut(s) 222, 411, 432, 453, 587, 623, 746, 910
StyI CCWWGG 1 cut(s) 878
TaaI ACNGT 2 cut(s) 450, 620
TaqI TCGA 7 cut(s) 201, 306, 327, 339, 576, 764, 1262
TatI WGTACW 2 cut(s) 509, 843
TauI GCSGC 2 cut(s) 152, 958
TfiI GAWTC 7 cut(s) 5, 86, 396, 557, 838, 1015, 1250
TscAI CASTG 1 cut(s) 1227
TseI GCWGC 3 cut(s) 603, 671, 938
TspDTI ATGAA 2 cut(s) 873, 1043
TspRI CASTG 1 cut(s) 1227
Van91I CCANNNNNTGG 1 cut(s) 682
VpaK11BI GGWCC 1 cut(s) 349
XapI RAATTY 2 cut(s) 702, 802
XceI RCATGY 1 cut(s) 776
XhoI CTCGAG 2 cut(s) 326, 338
XspI CTAG 2 cut(s) 948, 1272
ZrmI AGTACT 1 cut(s) 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.