Rmu_sc0010161.1_g000023
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0010161.1
OG0009649
Physical Location & Seq
Reverse (-)
41703 .. 42797
1095 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0010161.1_g000023.1.cds

Sequence Viewer

Length: 1095 bp
atgcaagcaggcctgtctcgactccttcgagcccactctcttggcccactcagcctccctctccctcgcactcccgactccttcaagacctctttccgaatgacctcctcaaccttcgagtggctctgctctttactcgagcccttactcgagtgcagggacccgattggctccccgctcaatctctccgccgacctccgcctcggaatcggcctgttccgcctggccaccggagccaattacccggagatatcaagacagttccgggtttccgagtctgtgtccagattttgctccaagcaattgtgtcgtgttctctgtactaactaccggttctggattgagtttccgaataagactgagcttgaatcggtgtcttcgggtttcgaaacccacaccgggttgcccaattgctgcggtgtgattgactgtacccggttcaagtttgtgagaagtaatggggttgctcaagaagaagaagttgcagcccagatcatggtggatgctaattccagaattctcagcattgtggctgggttcagggggaataaaagtgatgctagggttttgaagagttcgactctgtatgcagatgttgagggtgggaatttactgaattcggatccggttagtgtgaatggggtgccggtgaatcagtacttggttggagatgaagggtaccctttgcttccttggcttatggtgccctttgttgatgcaatgccggggtcggatgaggagcaattcaatgcagcacacactagaatgcggctttcggggcttagggctattgatagtttgaagaattggggggttttgagcataccgatttgcgaggagatgaagatggctgttgcttatattggtgcttgttctattctacataatggtttgttgatgagggaggactattcggcaatgtctgctgggttggatgattactctgtctatgatcagagctctcagtatcatagggacaattcgagtttggaggagagttcaattgagaggaaggcttctgttataagaaatgcactggctacaaaagcaaaagagtttcaagaatcaatattttcgacacattctagctcgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

364

Amino Acids

40.24

Weight (kDa)

5.63

Isoelectric Point (pI)

46.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1025
Acc65I GGTACC 1 cut(s) 678
AccB1I GGYRCC 3 cut(s) 643, 678, 703
AccB7I CCANNNNNTGG 1 cut(s) 496
AccBSI CCGCTC 1 cut(s) 178
AciI CCGC 6 cut(s) 176, 189, 199, 220, 417, 769
AclWI GGATC 2 cut(s) 617, 630
AcoI YGGCCR 1 cut(s) 225
AcsI RAATTY 3 cut(s) 516, 607, 616
AfaI GTAC 4 cut(s) 322, 433, 659, 680
AfiI CCNNNNNNNGG 3 cut(s) 120, 399, 496
AgeI ACCGGT 1 cut(s) 330
AgsI TTSAA 8 cut(s) 85, 368, 442, 571, 748, 802, 1002, 1061
AjnI CCWGG 1 cut(s) 222
AjuI GAANNNNNNNTTGG 2 cut(s) 644, 676
AluBI AGCT 3 cut(s) 364, 960, 1089
AluI AGCT 3 cut(s) 364, 960, 1089
Alw21I GWGCWC 1 cut(s) 962
Alw26I GTCTC 1 cut(s) 21
AlwI GGATC 2 cut(s) 617, 630
Ama87I CYCGRG 2 cut(s) 137, 149
AoxI GGCC 4 cut(s) 10, 43, 211, 225
ApeKI GCWGC 3 cut(s) 414, 485, 752
ApoI RAATTY 3 cut(s) 516, 607, 616
AsiGI ACCGGT 1 cut(s) 330
Asp718I GGTACC 1 cut(s) 678
AspS9I GGNCC 2 cut(s) 44, 160
AsuC2I CCSGG 5 cut(s) 245, 266, 400, 436, 726
AsuHPI GGTGA 1 cut(s) 661
AsuII TTCGAA 1 cut(s) 387
AvaI CYCGRG 2 cut(s) 137, 149
AvaII GGWCC 1 cut(s) 160
BaeGI GKGCMC 1 cut(s) 708
BalI TGGCCA 1 cut(s) 227
BamHI GGATCC 1 cut(s) 622
BanI GGYRCC 3 cut(s) 643, 678, 703
BanII GRGCYC 3 cut(s) 34, 144, 962
BbsI GAAGAC 1 cut(s) 369
Bbv12I GWGCWC 1 cut(s) 962
BbvI GCAGC 3 cut(s) 401, 497, 764
BccI CCATC 1 cut(s) 841
BcgI CGANNNNNNTGC 2 cut(s) 290, 324
BciT130I CCWGG 1 cut(s) 224
BclI TGATCA 1 cut(s) 952
BcnI CCSGG 5 cut(s) 245, 266, 400, 436, 726
BcoDI GTCTC 1 cut(s) 21
BfaI CTAG 3 cut(s) 561, 762, 1086
BisI GCNGC 4 cut(s) 415, 486, 753, 770
BlsI GCNGC 4 cut(s) 416, 487, 754, 771
BmcAI AGTACT 1 cut(s) 659
Bme1390I CCNGG 6 cut(s) 224, 245, 266, 400, 436, 726
Bme18I GGWCC 1 cut(s) 160
BmeT110I CYCGRG 2 cut(s) 137, 149
BmgT120I GGNCC 2 cut(s) 44, 160
BmiI GGNNCC 8 cut(s) 161, 162, 172, 235, 624, 645, 680, 705
BmrFI CCNGG 6 cut(s) 224, 245, 266, 400, 436, 726
BmsI GCATC 3 cut(s) 493, 547, 706
BpiI GAAGAC 1 cut(s) 369
BplI GAGNNNNNCTC 2 cut(s) 565, 597
Bpu10I CCTNAGC 1 cut(s) 782
Bpu14I TTCGAA 1 cut(s) 387
BpuEI CTTGAG 1 cut(s) 453
BpuMI CCSGG 5 cut(s) 245, 266, 400, 436, 726
BsaJI CCNNGG 3 cut(s) 202, 692, 725
BsaWI WCCGGW 3 cut(s) 230, 330, 625
BsaXI ACNNNNNCTCC 2 cut(s) 39, 69
Bsc4I CCNNNNNNNGG 3 cut(s) 120, 399, 496
Bse118I RCCGGY 2 cut(s) 330, 646
Bse1I ACTGG 1 cut(s) 1041
Bse3DI GCAATG 2 cut(s) 726, 924
BseBI CCWGG 1 cut(s) 224
BseDI CCNNGG 3 cut(s) 202, 692, 725
BseGI GGATG 3 cut(s) 508, 739, 940
BseLI CCNNNNNNNGG 3 cut(s) 120, 399, 496
BseMI GCAATG 2 cut(s) 726, 924
BseMII CTCAG 4 cut(s) 64, 351, 535, 977
BseNI ACTGG 1 cut(s) 1041
BseRI GAGGAG 4 cut(s) 97, 752, 851, 1007
BseSI GKGCMC 1 cut(s) 708
BseXI GCAGC 3 cut(s) 401, 497, 764
BseYI CCCAGC 2 cut(s) 533, 926
BsgI GTGCAG 1 cut(s) 175
BshFI GGCC 4 cut(s) 12, 45, 213, 227
BshNI GGYRCC 3 cut(s) 643, 678, 703
BshTI ACCGGT 1 cut(s) 330
BsiHKAI GWGCWC 1 cut(s) 962
BsiHKCI CYCGRG 2 cut(s) 137, 149
BsiSI CCGG 9 cut(s) 231, 245, 265, 331, 399, 436, 626, 647, 725
BslFI GGGAC 2 cut(s) 173, 989
BslI CCNNNNNNNGG 3 cut(s) 120, 399, 496
BsmAI GTCTC 1 cut(s) 21
BsmFI GGGAC 2 cut(s) 173, 989
BsmI GAATGC 1 cut(s) 771
BsnI GGCC 4 cut(s) 12, 45, 213, 227
BsoBI CYCGRG 2 cut(s) 137, 149
Bsp119I TTCGAA 1 cut(s) 387
Bsp1286I GDGCHC 4 cut(s) 34, 144, 708, 962
Bsp143I GATC 3 cut(s) 492, 622, 952
BspACI CCGC 6 cut(s) 176, 189, 199, 220, 417, 769
BspANI GGCC 4 cut(s) 12, 45, 213, 227
BspCNI CTCAG 4 cut(s) 63, 352, 534, 976
BspLI GGNNCC 8 cut(s) 161, 162, 172, 235, 624, 645, 680, 705
BspPI GGATC 2 cut(s) 617, 630
BspT104I TTCGAA 1 cut(s) 387
BspT107I GGYRCC 3 cut(s) 643, 678, 703
BsrBI CCGCTC 1 cut(s) 178
BsrDI GCAATG 2 cut(s) 726, 924
BsrFI RCCGGY 2 cut(s) 330, 646
BsrI ACTGG 1 cut(s) 1041
BssAI RCCGGY 2 cut(s) 330, 646
BssECI CCNNGG 3 cut(s) 202, 692, 725
BssMI GATC 3 cut(s) 492, 622, 952
BssT1I CCWWGG 1 cut(s) 692
Bst2UI CCWGG 1 cut(s) 224
Bst4CI ACNGT 2 cut(s) 261, 431
Bst6I CTCTTC 1 cut(s) 566
BstAPI GCANNNNNTGC 1 cut(s) 923
BstBI TTCGAA 1 cut(s) 387
BstC8I GCNNGC 2 cut(s) 6, 10
BstDEI CTNAG 5 cut(s) 50, 360, 521, 782, 963
BstF5I GGATG 3 cut(s) 508, 739, 940
BstKTI GATC 3 cut(s) 495, 625, 955
BstMAI GTCTC 1 cut(s) 21
BstMBI GATC 3 cut(s) 492, 622, 952
BstMWI GCNNNNNNNGC 8 cut(s) 51, 219, 233, 694, 703, 778, 923, 1046
BstNI CCWGG 1 cut(s) 224
BstSCI CCNGG 6 cut(s) 222, 243, 264, 398, 434, 724
BstSLI GKGCMC 1 cut(s) 708
BstV1I GCAGC 3 cut(s) 401, 497, 764
BstV2I GAAGAC 1 cut(s) 369
BstX2I RGATCY 1 cut(s) 622
BstXI CCANNNNNNTGG 1 cut(s) 41
BstYI RGATCY 1 cut(s) 622
BsuRI GGCC 4 cut(s) 12, 45, 213, 227
BtsCI GGATG 3 cut(s) 508, 739, 940
BtsIMutI CAGTG 1 cut(s) 1034
Cac8I GCNNGC 2 cut(s) 6, 10
Cfr10I RCCGGY 2 cut(s) 330, 646
Cfr13I GGNCC 2 cut(s) 44, 160
Csp6I GTAC 4 cut(s) 321, 432, 658, 679
CspAI ACCGGT 1 cut(s) 330
CviAII CATG 1 cut(s) 496
CviQI GTAC 4 cut(s) 321, 432, 658, 679
DdeI CTNAG 5 cut(s) 50, 360, 521, 782, 963
DpnI GATC 3 cut(s) 494, 624, 954
DpnII GATC 3 cut(s) 492, 622, 952
EaeI YGGCCR 1 cut(s) 225
Eam1104I CTCTTC 1 cut(s) 566
EarI CTCTTC 1 cut(s) 566
EciI GGCGGA 3 cut(s) 178, 188, 209
Ecl136II GAGCTC 1 cut(s) 960
Eco130I CCWWGG 1 cut(s) 692
Eco147I AGGCCT 1 cut(s) 12
Eco24I GRGCYC 3 cut(s) 34, 144, 962
Eco32I GATATC 1 cut(s) 252
Eco47I GGWCC 1 cut(s) 160
Eco53kI GAGCTC 1 cut(s) 960
Eco88I CYCGRG 2 cut(s) 137, 149
EcoICRI GAGCTC 1 cut(s) 960
EcoO109I RGGNCCY 1 cut(s) 160
EcoRI GAATTC 2 cut(s) 516, 616
EcoRII CCWGG 1 cut(s) 222
EcoRV GATATC 1 cut(s) 252
EcoT14I CCWWGG 1 cut(s) 692
EcoT38I GRGCYC 3 cut(s) 34, 144, 962
ErhI CCWWGG 1 cut(s) 692
FaeI CATG 1 cut(s) 499
FaiI YATR 9 cut(s) 497, 588, 701, 824, 861, 885, 951, 972, 1025
FaqI GGGAC 2 cut(s) 173, 989
FatI CATG 1 cut(s) 495
FauI CCCGC 1 cut(s) 183
FbaI TGATCA 1 cut(s) 952
Fnu4HI GCNGC 4 cut(s) 415, 486, 753, 770
FokI GGATG 3 cut(s) 515, 746, 947
FriOI GRGCYC 3 cut(s) 34, 144, 962
Fsp4HI GCNGC 4 cut(s) 415, 486, 753, 770
FspBI CTAG 3 cut(s) 561, 762, 1086
GluI GCNGC 4 cut(s) 415, 486, 753, 770
GsaI CCCAGC 2 cut(s) 537, 930
HaeIII GGCC 4 cut(s) 12, 45, 213, 227
HapII CCGG 9 cut(s) 231, 245, 265, 331, 399, 436, 626, 647, 725
Hin1II CATG 1 cut(s) 499
HinfI GANTC 8 cut(s) 21, 77, 207, 275, 368, 580, 652, 1064
HpaII CCGG 9 cut(s) 231, 245, 265, 331, 399, 436, 626, 647, 725
HphI GGTGA 1 cut(s) 661
Hpy188I TCNGA 7 cut(s) 98, 206, 274, 351, 622, 733, 957
Hpy188III TCNNGA 9 cut(s) 18, 74, 85, 255, 285, 337, 470, 513, 1061
HpyAV CCTTC 5 cut(s) 35, 91, 124, 668, 1006
HpyCH4III ACNGT 2 cut(s) 261, 431
HpyCH4V TGCA 7 cut(s) 4, 156, 485, 590, 719, 752, 1034
HpyF10VI GCNNNNNNNGC 8 cut(s) 51, 219, 233, 694, 703, 778, 923, 1046
HpyF3I CTNAG 5 cut(s) 50, 360, 521, 782, 963
Hsp92II CATG 1 cut(s) 499
KflI GGGWCCC 1 cut(s) 160
KpnI GGTACC 1 cut(s) 682
Ksp22I TGATCA 1 cut(s) 952
Kzo9I GATC 3 cut(s) 492, 622, 952
LmnI GCTCC 4 cut(s) 176, 233, 299, 739
Lsp1109I GCAGC 3 cut(s) 401, 497, 764
LweI GCATC 3 cut(s) 493, 547, 706
MaeI CTAG 3 cut(s) 561, 762, 1086
MalI GATC 3 cut(s) 494, 624, 954
MbiI CCGCTC 1 cut(s) 178
MboI GATC 3 cut(s) 492, 622, 952
MboII GAAGA 6 cut(s) 369, 485, 488, 583, 814, 856
MfeI CAATTG 3 cut(s) 302, 409, 1002
MflI RGATCY 1 cut(s) 622
MhlI GDGCHC 4 cut(s) 34, 144, 708, 962
MlsI TGGCCA 1 cut(s) 227
MluNI TGGCCA 1 cut(s) 227
MlyI GAGTC 4 cut(s) 15, 71, 284, 574
MmeI TCCRAC 3 cut(s) 646, 711, 912
Mox20I TGGCCA 1 cut(s) 227
MscI TGGCCA 1 cut(s) 227
MslI CAYNNNNRTG 1 cut(s) 764
Msp20I TGGCCA 1 cut(s) 227
MspI CCGG 9 cut(s) 231, 245, 265, 331, 399, 436, 626, 647, 725
MspR9I CCNGG 6 cut(s) 224, 245, 266, 400, 436, 726
MunI CAATTG 3 cut(s) 302, 409, 1002
Mva1269I GAATGC 1 cut(s) 771
MvaI CCWGG 1 cut(s) 224
MwoI GCNNNNNNNGC 8 cut(s) 51, 219, 233, 694, 703, 778, 923, 1046
NciI CCSGG 5 cut(s) 245, 266, 400, 436, 726
NdeII GATC 3 cut(s) 492, 622, 952
NlaIII CATG 1 cut(s) 499
NlaIV GGNNCC 8 cut(s) 161, 162, 172, 235, 624, 645, 680, 705
NspV TTCGAA 1 cut(s) 387
PaeR7I CTCGAG 2 cut(s) 137, 149
PceI AGGCCT 1 cut(s) 12
PcsI WCGNNNNNNNCGW 1 cut(s) 25
PctI GAATGC 1 cut(s) 771
PfeI GAWTC 4 cut(s) 207, 368, 652, 1064
PflMI CCANNNNNTGG 1 cut(s) 496
PinAI ACCGGT 1 cut(s) 330
PkrI GCNGC 4 cut(s) 416, 487, 754, 771
PleI GAGTC 4 cut(s) 15, 71, 283, 574
PpsI GAGTC 4 cut(s) 15, 71, 283, 574
PpuMI RGGWCCY 1 cut(s) 160
PsiI TTATAA 1 cut(s) 1025
Psp124BI GAGCTC 1 cut(s) 962
Psp5II RGGWCCY 1 cut(s) 160
Psp6I CCWGG 1 cut(s) 222
PspFI CCCAGC 2 cut(s) 533, 926
PspGI CCWGG 1 cut(s) 222
PspN4I GGNNCC 8 cut(s) 161, 162, 172, 235, 624, 645, 680, 705
PspPI GGNCC 2 cut(s) 44, 160
PspPPI RGGWCCY 1 cut(s) 160
PspXI VCTCGAGB 2 cut(s) 137, 149
PsuI RGATCY 1 cut(s) 622
RsaI GTAC 4 cut(s) 322, 433, 659, 680
RsaNI GTAC 4 cut(s) 321, 432, 658, 679
RseI CAYNNNNRTG 1 cut(s) 764
SacI GAGCTC 1 cut(s) 962
SatI GCNGC 4 cut(s) 415, 486, 753, 770
Sau3AI GATC 3 cut(s) 492, 622, 952
Sau96I GGNCC 2 cut(s) 44, 160
ScaI AGTACT 1 cut(s) 659
SchI GAGTC 4 cut(s) 15, 71, 284, 574
ScrFI CCNGG 6 cut(s) 224, 245, 266, 400, 436, 726
SduI GDGCHC 4 cut(s) 34, 144, 708, 962
SetI ASST 7 cut(s) 92, 107, 116, 198, 366, 962, 1091
SfaNI GCATC 3 cut(s) 493, 547, 706
Sfr274I CTCGAG 2 cut(s) 137, 149
SfuI TTCGAA 1 cut(s) 387
SinI GGWCC 1 cut(s) 160
SlaI CTCGAG 2 cut(s) 137, 149
SmiMI CAYNNNNRTG 1 cut(s) 764
SmlI CTYRAG 3 cut(s) 137, 149, 468
SmoI CTYRAG 3 cut(s) 137, 149, 468
SseBI AGGCCT 1 cut(s) 12
SsiI CCGC 6 cut(s) 176, 189, 199, 220, 417, 769
SspI AATATT 1 cut(s) 1071
SspMI CTAG 3 cut(s) 561, 762, 1086
SstI GAGCTC 1 cut(s) 962
StuI AGGCCT 1 cut(s) 12
StyD4I CCNGG 6 cut(s) 222, 243, 264, 398, 434, 724
StyI CCWWGG 1 cut(s) 692
TaaI ACNGT 2 cut(s) 261, 431
TaqI TCGA 9 cut(s) 19, 28, 117, 138, 150, 387, 578, 983, 1076
TatI WGTACW 2 cut(s) 320, 657
TauI GCSGC 1 cut(s) 772
TfiI GAWTC 4 cut(s) 207, 368, 652, 1064
TscAI CASTG 1 cut(s) 1041
TseI GCWGC 3 cut(s) 414, 485, 752
TspDTI ATGAA 2 cut(s) 687, 857
TspRI CASTG 1 cut(s) 1041
Van91I CCANNNNNTGG 1 cut(s) 496
VpaK11BI GGWCC 1 cut(s) 160
XapI RAATTY 3 cut(s) 516, 607, 616
XhoI CTCGAG 2 cut(s) 137, 149
XspI CTAG 3 cut(s) 561, 762, 1086
ZrmI AGTACT 1 cut(s) 659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.