MD05G1083600.v1.1
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009649
Physical Location & Seq
Forward (+)
17447919 .. 17449209
1291 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1083600.v1.1.491

Sequence Viewer

Length: 1263 bp
ATGGAATCCGCCACCCTCGTAGCTCTCCTCTCTTCCATCTCCCAACTCATCGTCCTCCTCCTCCTCTCTCCCAACTCCGCCGATTCCAATTCCGCCGCTAATGCCTTCCCAATCGTCAACAATTTCCTCTCATCCCACGAAACCGCCGCCGCCCTCTCCCTCCTCACCCTTTCCCGGAAACGAAAGCGAACCCATTTCTCAGAACGGGATTCTGAGCCCGATGACCATGAGAGCGATCATGAACTTGGCGGCGGTGACTCAGTCCGACTCGGTCTGAGTCGGAGCCCGGACTCGTTCAGAAACTGCTTCAGAATGACCTCGTCCACCTTCGAATGGCTCTGCGGGTTACTCGAGCCGTTGCTCGAGTGTCGTGACCCAGTTGGGTCGCCTCTCAATCTCTCTGCCGATCTCCGTCTCGGTATGGGCTTGTTTCGGCTGTCCACCGGCTCCAGCTACCCCGAAATTTCAAAGCAATTCGGGGTTTCGGAGATGGTGGCCAGGTTCTGCGCCAAGCAATTGTGCCGAGTTCTTTGCACCAATTACCGGTTCTGGATTGAATTTCCCAACCCAATTGAGCTTGATTCTGTATCGGCGGCTTTCGAAACCCAAACTGGTTTGCCCAATTGTTGCGGTGTGATTGACTGCACAAGGTTTAAAATTGTTAGAAATGGTGGGGTGCAAGAAGAAAGCATTGCTGCCCAAATCACGGTGGATTCTTCGTCACGAATTCTCAGCATAGTCGCCGGTTTTCGTGGCAATAAGGGTGATTCAAGAGTACTAAGGTCGTCGACTTTGTACAAGGATATTGAAGCGGGAAAGTTGTTGAATTCTCCTCCGGCTTCTGTCAATGGGGTGGCTGTGAATCAGTACTTGATAGGAGATGGAGGGTATCCTTTGCTTCCTTGGCTTATGGTCCCTTTCGTTGATGCCGTGAAGGGCTCACCCGAAGAGCATTTCAATGCAGCACACAATGTGATGCGCCTTTCGGCGCTTAGGACGATTGTAAGTCTGAAGAATTGGGGTGTTTTGAGCCGACCAATACAAGAGGAGATGAAGATGGCAGTGGCTTATATCGGCGCTTGTTCGATATTGCATAATGGTTTGTTGAGGAGGGAGGACTTTTCTGCATTGTGTGATGGGTTGGATGATTATTCCTTGTATGATCAGAGCTCTCAGTATTATAGGGATACTAGTTTGGAGGAGAATTCGATTGAGAGGAAGGCTTCTGTTATTCGAAGTGCATTGGCTACGAAAGCGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

421

Amino Acids

46.05

Weight (kDa)

5.89

Isoelectric Point (pI)

53.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 228 - 366 4.7e-16 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 788
AcoI YGGCCR 1 cut(s) 495
AcsI RAATTY 5 cut(s) 462, 557, 726, 826, 1204
AcuI CTGAAG 2 cut(s) 292, 1031
AdeI CACNNNGTG 1 cut(s) 973
AfaI GTAC 3 cut(s) 777, 797, 869
AfiI CCNNNNNNNGG 4 cut(s) 174, 333, 543, 706
AgeI ACCGGT 1 cut(s) 543
AgsI TTSAA 6 cut(s) 468, 557, 771, 809, 826, 958
AhlI ACTAGT 1 cut(s) 1190
AjnI CCWGG 1 cut(s) 497
AjuI GAANNNNNNNTTGG 2 cut(s) 530, 562
AluBI AGCT 4 cut(s) 23, 453, 577, 1170
AluI AGCT 4 cut(s) 23, 453, 577, 1170
Alw21I GWGCWC 1 cut(s) 1172
Alw26I GTCTC 1 cut(s) 419
AlwNI CAGNNNCTG 2 cut(s) 303, 504
Ama87I CYCGRG 2 cut(s) 350, 362
AoxI GGCC 1 cut(s) 495
ApeKI GCWGC 2 cut(s) 695, 962
ApoI RAATTY 5 cut(s) 462, 557, 726, 826, 1204
AsiGI ACCGGT 1 cut(s) 543
AspLEI GCGC 4 cut(s) 509, 981, 991, 1079
AspS9I GGNCC 1 cut(s) 913
AsuC2I CCSGG 2 cut(s) 175, 287
AsuHPI GGTGA 4 cut(s) 157, 266, 776, 933
AsuII TTCGAA 3 cut(s) 330, 600, 1234
AvaI CYCGRG 2 cut(s) 350, 362
AvaII GGWCC 1 cut(s) 913
BalI TGGCCA 1 cut(s) 497
BanII GRGCYC 4 cut(s) 219, 287, 941, 1172
Bbv12I GWGCWC 1 cut(s) 1172
BbvI GCAGC 2 cut(s) 682, 974
BccI CCATC 5 cut(s) 44, 484, 875, 1051, 1130
BceAI ACGGC 2 cut(s) 340, 914
BcgI CGANNNNNNTGC 2 cut(s) 513, 547
BciT130I CCWGG 1 cut(s) 499
BciVI GTATCC 2 cut(s) 900, 1180
BclI TGATCA 1 cut(s) 1162
BcnI CCSGG 2 cut(s) 175, 287
BcoDI GTCTC 1 cut(s) 419
BcuI ACTAGT 1 cut(s) 1190
BfaI CTAG 1 cut(s) 1191
BfoI RGCGCY 2 cut(s) 992, 1080
BfuI GTATCC 2 cut(s) 900, 1180
BisI GCNGC 7 cut(s) 96, 147, 150, 250, 594, 696, 963
BlsI GCNGC 7 cut(s) 97, 148, 151, 251, 595, 697, 964
BmcAI AGTACT 2 cut(s) 777, 869
Bme1390I CCNGG 3 cut(s) 175, 287, 499
Bme18I GGWCC 1 cut(s) 913
BmeT110I CYCGRG 2 cut(s) 350, 362
BmgT120I GGNCC 1 cut(s) 913
BmiI GGNNCC 3 cut(s) 284, 448, 915
BmrFI CCNGG 3 cut(s) 175, 287, 499
BmrI ACTGGG 1 cut(s) 371
BmsI GCATC 2 cut(s) 916, 966
BmuI ACTGGG 1 cut(s) 371
BpmI CTGGAG 1 cut(s) 433
Bpu10I CCTNAGC 1 cut(s) 992
Bpu14I TTCGAA 3 cut(s) 330, 600, 1234
BpuMI CCSGG 2 cut(s) 175, 287
BsaJI CCNNGG 1 cut(s) 902
BsaWI WCCGGW 1 cut(s) 543
Bsc4I CCNNNNNNNGG 4 cut(s) 174, 333, 543, 706
Bse118I RCCGGY 3 cut(s) 443, 543, 743
Bse1I ACTGG 2 cut(s) 377, 616
Bse3DI GCAATG 1 cut(s) 690
BseBI CCWGG 1 cut(s) 499
BseDI CCNNGG 1 cut(s) 902
BseGI GGATG 2 cut(s) 131, 1150
BseLI CCNNNNNNNGG 4 cut(s) 174, 333, 543, 706
BseMI GCAATG 1 cut(s) 690
BseMII CTCAG 6 cut(s) 204, 213, 266, 273, 745, 1187
BseNI ACTGG 2 cut(s) 377, 616
BseRI GAGGAG 9 cut(s) 17, 47, 50, 53, 152, 822, 1061, 1123, 1214
BseXI GCAGC 2 cut(s) 682, 974
BsgI GTGCAG 1 cut(s) 628
BshFI GGCC 1 cut(s) 497
BshTI ACCGGT 1 cut(s) 543
BsiHKAI GWGCWC 1 cut(s) 1172
BsiHKCI CYCGRG 2 cut(s) 350, 362
BsiSI CCGG 6 cut(s) 175, 287, 444, 544, 744, 836
BslFI GGGAC 1 cut(s) 899
BslI CCNNNNNNNGG 4 cut(s) 174, 333, 543, 706
BsmAI GTCTC 1 cut(s) 419
BsmBI CGTCTC 1 cut(s) 419
BsmFI GGGAC 1 cut(s) 899
BsnI GGCC 1 cut(s) 497
BsoBI CYCGRG 2 cut(s) 350, 362
Bsp119I TTCGAA 3 cut(s) 330, 600, 1234
Bsp1286I GDGCHC 4 cut(s) 219, 287, 941, 1172
Bsp1407I TGTACA 1 cut(s) 795
Bsp143I GATC 3 cut(s) 235, 406, 1162
BspANI GGCC 1 cut(s) 497
BspCNI CTCAG 6 cut(s) 205, 212, 267, 272, 744, 1186
BspHI TCATGA 1 cut(s) 238
BspLI GGNNCC 3 cut(s) 284, 448, 915
BspQI GCTCTTC 1 cut(s) 942
BspT104I TTCGAA 3 cut(s) 330, 600, 1234
BsrDI GCAATG 1 cut(s) 690
BsrFI RCCGGY 3 cut(s) 443, 543, 743
BsrGI TGTACA 1 cut(s) 795
BsrI ACTGG 2 cut(s) 377, 616
BssAI RCCGGY 3 cut(s) 443, 543, 743
BssECI CCNNGG 1 cut(s) 902
BssMI GATC 3 cut(s) 235, 406, 1162
BssT1I CCWWGG 1 cut(s) 902
Bst2UI CCWGG 1 cut(s) 499
Bst4CI ACNGT 1 cut(s) 709
Bst6I CTCTTC 2 cut(s) 37, 942
BstAUI TGTACA 1 cut(s) 795
BstBI TTCGAA 3 cut(s) 330, 600, 1234
BstDEI CTNAG 8 cut(s) 199, 213, 259, 275, 731, 779, 992, 1173
BstF5I GGATG 2 cut(s) 131, 1150
BstH2I RGCGCY 2 cut(s) 992, 1080
BstHHI GCGC 4 cut(s) 509, 981, 991, 1079
BstKTI GATC 3 cut(s) 238, 409, 1165
BstMAI GTCTC 1 cut(s) 419
BstMBI GATC 3 cut(s) 235, 406, 1162
BstMWI GCNNNNNNNGC 3 cut(s) 101, 904, 1253
BstNI CCWGG 1 cut(s) 499
BstSCI CCNGG 3 cut(s) 173, 285, 497
BstV1I GCAGC 2 cut(s) 682, 974
BsuI GTATCC 2 cut(s) 900, 1180
BsuRI GGCC 1 cut(s) 497
BtsCI GGATG 2 cut(s) 131, 1150
BtsI GCAGTG 1 cut(s) 1068
BtsIMutI CAGTG 1 cut(s) 1068
CaiI CAGNNNCTG 2 cut(s) 303, 504
CciI TCATGA 1 cut(s) 238
CfoI GCGC 4 cut(s) 509, 981, 991, 1079
Cfr10I RCCGGY 3 cut(s) 443, 543, 743
Cfr13I GGNCC 1 cut(s) 913
Csp6I GTAC 3 cut(s) 776, 796, 868
CspAI ACCGGT 1 cut(s) 543
CviAII CATG 2 cut(s) 227, 239
CviQI GTAC 3 cut(s) 776, 796, 868
DdeI CTNAG 8 cut(s) 199, 213, 259, 275, 731, 779, 992, 1173
DpnI GATC 3 cut(s) 237, 408, 1164
DpnII GATC 3 cut(s) 235, 406, 1162
DraI TTTAAA 1 cut(s) 655
DraIII CACNNNGTG 1 cut(s) 973
EaeI YGGCCR 1 cut(s) 495
Eam1104I CTCTTC 2 cut(s) 37, 942
EarI CTCTTC 2 cut(s) 37, 942
EciI GGCGGA 2 cut(s) 67, 82
Ecl136II GAGCTC 1 cut(s) 1170
Eco130I CCWWGG 1 cut(s) 902
Eco24I GRGCYC 4 cut(s) 219, 287, 941, 1172
Eco47I GGWCC 1 cut(s) 913
Eco53kI GAGCTC 1 cut(s) 1170
Eco57I CTGAAG 2 cut(s) 292, 1031
Eco88I CYCGRG 2 cut(s) 350, 362
EcoICRI GAGCTC 1 cut(s) 1170
EcoRI GAATTC 3 cut(s) 726, 826, 1204
EcoRII CCWGG 1 cut(s) 497
EcoT14I CCWWGG 1 cut(s) 902
EcoT38I GRGCYC 4 cut(s) 219, 287, 941, 1172
ErhI CCWWGG 1 cut(s) 902
Esp3I CGTCTC 1 cut(s) 419
FaeI CATG 2 cut(s) 230, 242
FaiI YATR 9 cut(s) 228, 240, 422, 737, 911, 1071, 1095, 1161, 1182
FaqI GGGAC 1 cut(s) 899
FatI CATG 2 cut(s) 226, 238
FauI CCCGC 2 cut(s) 335, 805
FbaI TGATCA 1 cut(s) 1162
FblI GTMKAC 1 cut(s) 788
Fnu4HI GCNGC 7 cut(s) 96, 147, 150, 250, 594, 696, 963
FokI GGATG 2 cut(s) 118, 1157
FriOI GRGCYC 4 cut(s) 219, 287, 941, 1172
Fsp4HI GCNGC 7 cut(s) 96, 147, 150, 250, 594, 696, 963
FspBI CTAG 1 cut(s) 1191
GlaI GCGC 4 cut(s) 508, 980, 990, 1078
GluI GCNGC 7 cut(s) 96, 147, 150, 250, 594, 696, 963
GsuI CTGGAG 1 cut(s) 433
HaeII RGCGCY 2 cut(s) 992, 1080
HaeIII GGCC 1 cut(s) 497
HapII CCGG 6 cut(s) 175, 287, 444, 544, 744, 836
HhaI GCGC 4 cut(s) 509, 981, 991, 1079
Hin1II CATG 2 cut(s) 230, 242
Hin6I GCGC 4 cut(s) 507, 979, 989, 1077
HinP1I GCGC 4 cut(s) 507, 979, 989, 1077
HincII GTYRAC 2 cut(s) 118, 789
HindII GTYRAC 2 cut(s) 118, 789
HpaII CCGG 6 cut(s) 175, 287, 444, 544, 744, 836
HphI GGTGA 4 cut(s) 157, 266, 776, 933
Hpy166II GTNNAC 4 cut(s) 118, 324, 441, 789
Hpy188III TCNNGA 5 cut(s) 239, 371, 550, 723, 771
Hpy8I GTNNAC 4 cut(s) 118, 324, 441, 789
Hpy99I CGWCG 1 cut(s) 790
HpyAV CCTTC 4 cut(s) 115, 337, 928, 1213
HpyCH4III ACNGT 1 cut(s) 709
HpyCH4V TGCA 7 cut(s) 534, 645, 679, 962, 1093, 1127, 1241
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 904, 1253
HpyF3I CTNAG 8 cut(s) 199, 213, 259, 275, 731, 779, 992, 1173
Hsp92II CATG 2 cut(s) 230, 242
HspAI GCGC 4 cut(s) 507, 979, 989, 1077
Ksp22I TGATCA 1 cut(s) 1162
Kzo9I GATC 3 cut(s) 235, 406, 1162
LguI GCTCTTC 1 cut(s) 942
LmnI GCTCC 2 cut(s) 282, 452
Lsp1109I GCAGC 2 cut(s) 682, 974
LweI GCATC 2 cut(s) 916, 966
MaeI CTAG 1 cut(s) 1191
MaeIII GTNAC 4 cut(s) 254, 345, 371, 720
MalI GATC 3 cut(s) 237, 408, 1164
MboI GATC 3 cut(s) 235, 406, 1162
MboII GAAGA 6 cut(s) 24, 695, 708, 959, 1024, 1066
MfeI CAATTG 3 cut(s) 515, 570, 622
MhlI GDGCHC 4 cut(s) 219, 287, 941, 1172
MlsI TGGCCA 1 cut(s) 497
MluNI TGGCCA 1 cut(s) 497
MlyI GAGTC 4 cut(s) 251, 261, 284, 286
MmeI TCCRAC 3 cut(s) 260, 289, 1122
Mox20I TGGCCA 1 cut(s) 497
MscI TGGCCA 1 cut(s) 497
MseI TTAA 1 cut(s) 654
MslI CAYNNNNRTG 1 cut(s) 957
Msp20I TGGCCA 1 cut(s) 497
MspI CCGG 6 cut(s) 175, 287, 444, 544, 744, 836
MspR9I CCNGG 3 cut(s) 175, 287, 499
MunI CAATTG 3 cut(s) 515, 570, 622
MvaI CCWGG 1 cut(s) 499
MwoI GCNNNNNNNGC 3 cut(s) 101, 904, 1253
NciI CCSGG 2 cut(s) 175, 287
NdeII GATC 3 cut(s) 235, 406, 1162
NlaIII CATG 2 cut(s) 230, 242
NlaIV GGNNCC 3 cut(s) 284, 448, 915
NmeAIII GCCGAG 1 cut(s) 548
NmuCI GTSAC 3 cut(s) 254, 371, 720
NspV TTCGAA 3 cut(s) 330, 600, 1234
PaeR7I CTCGAG 2 cut(s) 350, 362
PagI TCATGA 1 cut(s) 238
PciSI GCTCTTC 1 cut(s) 942
PcsI WCGNNNNNNNCGW 1 cut(s) 927
PfeI GAWTC 7 cut(s) 5, 83, 209, 581, 713, 767, 862
PflFI GACNNNGTC 3 cut(s) 260, 270, 319
PfoI TCCNGGA 1 cut(s) 173
PinAI ACCGGT 1 cut(s) 543
PkrI GCNGC 7 cut(s) 97, 148, 151, 251, 595, 697, 964
PleI GAGTC 4 cut(s) 251, 261, 284, 285
PpsI GAGTC 4 cut(s) 251, 261, 284, 285
Psp124BI GAGCTC 1 cut(s) 1172
Psp6I CCWGG 1 cut(s) 497
PspGI CCWGG 1 cut(s) 497
PspN4I GGNNCC 3 cut(s) 284, 448, 915
PspPI GGNCC 1 cut(s) 913
PspXI VCTCGAGB 2 cut(s) 350, 362
PstNI CAGNNNCTG 2 cut(s) 303, 504
PsyI GACNNNGTC 3 cut(s) 260, 270, 319
RsaI GTAC 3 cut(s) 777, 797, 869
RsaNI GTAC 3 cut(s) 776, 796, 868
RseI CAYNNNNRTG 1 cut(s) 957
SacI GAGCTC 1 cut(s) 1172
SalI GTCGAC 1 cut(s) 787
SapI GCTCTTC 1 cut(s) 942
SaqAI TTAA 1 cut(s) 654
SatI GCNGC 7 cut(s) 96, 147, 150, 250, 594, 696, 963
Sau3AI GATC 3 cut(s) 235, 406, 1162
Sau96I GGNCC 1 cut(s) 913
ScaI AGTACT 2 cut(s) 777, 869
SchI GAGTC 4 cut(s) 251, 261, 284, 286
ScrFI CCNGG 3 cut(s) 175, 287, 499
SduI GDGCHC 4 cut(s) 219, 287, 941, 1172
SetI ASST 9 cut(s) 25, 320, 329, 455, 503, 579, 653, 785, 1172
SfaNI GCATC 2 cut(s) 916, 966
Sfr274I CTCGAG 2 cut(s) 350, 362
SfuI TTCGAA 3 cut(s) 330, 600, 1234
SinI GGWCC 1 cut(s) 913
SlaI CTCGAG 2 cut(s) 350, 362
SmiMI CAYNNNNRTG 1 cut(s) 957
SmlI CTYRAG 2 cut(s) 350, 362
SmoI CTYRAG 2 cut(s) 350, 362
SpeI ACTAGT 1 cut(s) 1190
SspMI CTAG 1 cut(s) 1191
SstI GAGCTC 1 cut(s) 1172
StyD4I CCNGG 3 cut(s) 173, 285, 497
StyI CCWWGG 1 cut(s) 902
TaaI ACNGT 1 cut(s) 709
TaqI TCGA 8 cut(s) 330, 351, 363, 600, 788, 1085, 1208, 1234
TaqII GACCGA 1 cut(s) 260
TatI WGTACW 3 cut(s) 775, 795, 867
TauI GCSGC 5 cut(s) 98, 149, 152, 252, 596
TfiI GAWTC 7 cut(s) 5, 83, 209, 581, 713, 767, 862
Tru1I TTAA 1 cut(s) 654
Tru9I TTAA 1 cut(s) 654
TscAI CASTG 1 cut(s) 1068
TseFI GTSAC 3 cut(s) 254, 371, 720
TseI GCWGC 2 cut(s) 695, 962
Tsp45I GTSAC 3 cut(s) 254, 371, 720
TspDTI ATGAA 2 cut(s) 255, 1067
TspGWI ACGGA 1 cut(s) 401
TspRI CASTG 1 cut(s) 1068
Tth111I GACNNNGTC 3 cut(s) 260, 270, 319
VpaK11BI GGWCC 1 cut(s) 913
XapI RAATTY 5 cut(s) 462, 557, 726, 826, 1204
XhoI CTCGAG 2 cut(s) 350, 362
XmiI GTMKAC 1 cut(s) 788
XspI CTAG 1 cut(s) 1191
ZrmI AGTACT 2 cut(s) 777, 869
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.