Rh7DG177100
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0009649
Physical Location & Seq
Forward (+)
16309105 .. 16312014
2910 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG177100.1

Sequence Viewer

Length: 420 bp
ATGACCTCCTCAACCTTCGAGTGGCTCTGCACCTTGCTCGAGCCCTTACTCGAGTGCAGGGACCCGATTGGCTTCCCGCTCAATCTCTCCCCCAACCTCGGCCTTGGAATCCGCATGTTCCGCCTGGCCACCGGAGCCAATTACCTAGAGATATCAAGACAATTCCGGGTTTCGGAGTCTGTGTCGAGATTTTACTCCAAGCAATTGTGTCGTGTTCTCTGCACTAACTACCGATTCTGGATTGAGTTTCTGAATAAGACTGAGCTTGAATCGGTGTCTTCGAAACCCACACCGGGTTGCCCAATTGTTGCGAACCTTCTAGAAAACACAAAGCCTCCCTCCCGCTCTCTGTTTCTGTTCGCCGCCGCTTCTTCCTCCGCCGGTGAAGCTTTTCGTTTGAAGAACCCAACACTTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

139

Amino Acids

15.64

Weight (kDa)

8.95

Isoelectric Point (pI)

50.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 79, 345
AciI CCGC 7 cut(s) 77, 112, 121, 343, 363, 366, 378
AcoI YGGCCR 1 cut(s) 126
AfiI CCNNNNNNNGG 4 cut(s) 21, 98, 172, 293
AgsI TTSAA 2 cut(s) 269, 400
AjnI CCWGG 1 cut(s) 123
AluBI AGCT 2 cut(s) 265, 389
AluI AGCT 2 cut(s) 265, 389
Ama87I CYCGRG 2 cut(s) 38, 50
AoxI GGCC 2 cut(s) 100, 126
Asp700I GAANNNNTTC 1 cut(s) 390
AspS9I GGNCC 1 cut(s) 61
AsuC2I CCSGG 2 cut(s) 167, 294
AsuHPI GGTGA 1 cut(s) 395
AsuII TTCGAA 1 cut(s) 281
AvaI CYCGRG 2 cut(s) 38, 50
AvaII GGWCC 1 cut(s) 61
BalI TGGCCA 1 cut(s) 128
BanII GRGCYC 1 cut(s) 45
BbsI GAAGAC 1 cut(s) 270
BcgI CGANNNNNNTGC 4 cut(s) 19, 53, 191, 225
BciT130I CCWGG 1 cut(s) 125
BcnI CCSGG 2 cut(s) 167, 294
BfaI CTAG 2 cut(s) 146, 320
BisI GCNGC 2 cut(s) 363, 366
BlsI GCNGC 2 cut(s) 364, 367
Bme1390I CCNGG 3 cut(s) 125, 167, 294
Bme18I GGWCC 1 cut(s) 61
BmeT110I CYCGRG 2 cut(s) 38, 50
BmgT120I GGNCC 1 cut(s) 61
BmiI GGNNCC 3 cut(s) 62, 63, 136
BmrFI CCNGG 3 cut(s) 125, 167, 294
BpiI GAAGAC 1 cut(s) 270
Bpu14I TTCGAA 1 cut(s) 281
BpuMI CCSGG 2 cut(s) 167, 294
BsaJI CCNNGG 2 cut(s) 97, 103
BsaWI WCCGGW 1 cut(s) 131
BsaXI ACNNNNNCTCC 4 cut(s) 167, 197, 319, 349
Bsc4I CCNNNNNNNGG 4 cut(s) 21, 98, 172, 293
Bse118I RCCGGY 1 cut(s) 380
BseBI CCWGG 1 cut(s) 125
BseDI CCNNGG 2 cut(s) 97, 103
BseLI CCNNNNNNNGG 4 cut(s) 21, 98, 172, 293
BseMII CTCAG 1 cut(s) 252
BsgI GTGCAG 3 cut(s) 13, 76, 205
BshFI GGCC 2 cut(s) 102, 128
BsiHKCI CYCGRG 2 cut(s) 38, 50
BsiSI CCGG 4 cut(s) 132, 166, 293, 381
BslFI GGGAC 1 cut(s) 74
BslI CCNNNNNNNGG 4 cut(s) 21, 98, 172, 293
BsmFI GGGAC 1 cut(s) 74
BsnI GGCC 2 cut(s) 102, 128
BsoBI CYCGRG 2 cut(s) 38, 50
Bsp119I TTCGAA 1 cut(s) 281
Bsp1286I GDGCHC 1 cut(s) 45
BspACI CCGC 7 cut(s) 77, 112, 121, 343, 363, 366, 378
BspANI GGCC 2 cut(s) 102, 128
BspCNI CTCAG 1 cut(s) 253
BspLI GGNNCC 3 cut(s) 62, 63, 136
BspT104I TTCGAA 1 cut(s) 281
BsrBI CCGCTC 2 cut(s) 79, 345
BsrFI RCCGGY 1 cut(s) 380
BssAI RCCGGY 1 cut(s) 380
BssECI CCNNGG 2 cut(s) 97, 103
BssT1I CCWWGG 1 cut(s) 103
Bst2UI CCWGG 1 cut(s) 125
BstBI TTCGAA 1 cut(s) 281
BstDEI CTNAG 1 cut(s) 261
BstMWI GCNNNNNNNGC 3 cut(s) 120, 134, 386
BstNI CCWGG 1 cut(s) 125
BstNSI RCATGY 1 cut(s) 118
BstSCI CCNGG 3 cut(s) 123, 165, 292
BstV2I GAAGAC 1 cut(s) 270
BsuRI GGCC 2 cut(s) 102, 128
Cfr10I RCCGGY 1 cut(s) 380
Cfr13I GGNCC 1 cut(s) 61
CviAII CATG 1 cut(s) 115
CviJI RGCY 9 cut(s) 25, 43, 72, 102, 128, 137, 265, 334, 389
CviKI_1 RGCY 9 cut(s) 25, 43, 72, 102, 128, 137, 265, 334, 389
DdeI CTNAG 1 cut(s) 261
EaeI YGGCCR 1 cut(s) 126
EciI GGCGGA 2 cut(s) 110, 367
Eco130I CCWWGG 1 cut(s) 103
Eco24I GRGCYC 1 cut(s) 45
Eco32I GATATC 1 cut(s) 153
Eco47I GGWCC 1 cut(s) 61
Eco88I CYCGRG 2 cut(s) 38, 50
EcoO109I RGGNCCY 1 cut(s) 61
EcoRII CCWGG 1 cut(s) 123
EcoRV GATATC 1 cut(s) 153
EcoT14I CCWWGG 1 cut(s) 103
EcoT38I GRGCYC 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 103
FaeI CATG 1 cut(s) 118
FaiI YATR 1 cut(s) 116
FaqI GGGAC 1 cut(s) 74
FatI CATG 1 cut(s) 114
FauI CCCGC 2 cut(s) 84, 350
Fnu4HI GCNGC 2 cut(s) 363, 366
FriOI GRGCYC 1 cut(s) 45
Fsp4HI GCNGC 2 cut(s) 363, 366
FspBI CTAG 2 cut(s) 146, 320
GluI GCNGC 2 cut(s) 363, 366
HaeIII GGCC 2 cut(s) 102, 128
HapII CCGG 4 cut(s) 132, 166, 293, 381
Hin1II CATG 1 cut(s) 118
HindIII AAGCTT 1 cut(s) 387
HinfI GANTC 4 cut(s) 108, 176, 234, 269
HpaII CCGG 4 cut(s) 132, 166, 293, 381
HphI GGTGA 1 cut(s) 395
Hpy188I TCNGA 2 cut(s) 175, 252
Hpy188III TCNNGA 4 cut(s) 156, 186, 238, 320
HpyAV CCTTC 2 cut(s) 25, 326
HpyCH4V TGCA 3 cut(s) 30, 57, 222
HpyF10VI GCNNNNNNNGC 3 cut(s) 120, 134, 386
HpyF3I CTNAG 1 cut(s) 261
Hsp92II CATG 1 cut(s) 118
KflI GGGWCCC 1 cut(s) 61
LmnI GCTCC 1 cut(s) 134
LpnPI CCDG 8 cut(s) 43, 110, 137, 145, 179, 223, 306, 394
MaeI CTAG 2 cut(s) 146, 320
MbiI CCGCTC 2 cut(s) 79, 345
MboII GAAGA 3 cut(s) 270, 363, 412
MfeI CAATTG 2 cut(s) 203, 303
MhlI GDGCHC 1 cut(s) 45
MlsI TGGCCA 1 cut(s) 128
MluCI AATT 4 cut(s) 139, 161, 203, 303
MluNI TGGCCA 1 cut(s) 128
MlyI GAGTC 1 cut(s) 185
MnlI CCTC 6 cut(s) 16, 19, 107, 345, 349, 385
Mox20I TGGCCA 1 cut(s) 128
MroXI GAANNNNTTC 1 cut(s) 390
MscI TGGCCA 1 cut(s) 128
Msp20I TGGCCA 1 cut(s) 128
MspI CCGG 4 cut(s) 132, 166, 293, 381
MspR9I CCNGG 3 cut(s) 125, 167, 294
MunI CAATTG 2 cut(s) 203, 303
MvaI CCWGG 1 cut(s) 125
MwoI GCNNNNNNNGC 3 cut(s) 120, 134, 386
NciI CCSGG 2 cut(s) 167, 294
NlaIII CATG 1 cut(s) 118
NlaIV GGNNCC 3 cut(s) 62, 63, 136
NmeAIII GCCGAG 1 cut(s) 78
NspI RCATGY 1 cut(s) 118
NspV TTCGAA 1 cut(s) 281
PaeR7I CTCGAG 2 cut(s) 38, 50
PcsI WCGNNNNNNNCGW 1 cut(s) 278
PdmI GAANNNNTTC 1 cut(s) 390
PfeI GAWTC 3 cut(s) 108, 234, 269
PkrI GCNGC 2 cut(s) 364, 367
PleI GAGTC 1 cut(s) 184
PpsI GAGTC 1 cut(s) 184
PpuMI RGGWCCY 1 cut(s) 61
Psp5II RGGWCCY 1 cut(s) 61
Psp6I CCWGG 1 cut(s) 123
PspGI CCWGG 1 cut(s) 123
PspN4I GGNNCC 3 cut(s) 62, 63, 136
PspPI GGNCC 1 cut(s) 61
PspPPI RGGWCCY 1 cut(s) 61
PspXI VCTCGAGB 2 cut(s) 38, 50
SatI GCNGC 2 cut(s) 363, 366
Sau96I GGNCC 1 cut(s) 61
SchI GAGTC 1 cut(s) 185
ScrFI CCNGG 3 cut(s) 125, 167, 294
SduI GDGCHC 1 cut(s) 45
SetI ASST 8 cut(s) 8, 17, 35, 99, 147, 267, 318, 391
Sfr274I CTCGAG 2 cut(s) 38, 50
SfuI TTCGAA 1 cut(s) 281
SgrAI CRCCGGYG 1 cut(s) 380
SinI GGWCC 1 cut(s) 61
SlaI CTCGAG 2 cut(s) 38, 50
SmlI CTYRAG 2 cut(s) 38, 50
SmoI CTYRAG 2 cut(s) 38, 50
Sse9I AATT 4 cut(s) 139, 161, 203, 303
SsiI CCGC 7 cut(s) 77, 112, 121, 343, 363, 366, 378
SspMI CTAG 2 cut(s) 146, 320
StyD4I CCNGG 3 cut(s) 123, 165, 292
StyI CCWWGG 1 cut(s) 103
TaqI TCGA 5 cut(s) 18, 39, 51, 185, 281
TasI AATT 4 cut(s) 139, 161, 203, 303
TauI GCSGC 2 cut(s) 365, 368
TfiI GAWTC 3 cut(s) 108, 234, 269
VpaK11BI GGWCC 1 cut(s) 61
XbaI TCTAGA 1 cut(s) 319
XceI RCATGY 1 cut(s) 118
XhoI CTCGAG 2 cut(s) 38, 50
XmnI GAANNNNTTC 1 cut(s) 390
XspI CTAG 2 cut(s) 146, 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.