Prupe.8G128000_v2.0.a1
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0009649
Physical Location & Seq
Forward (+)
15180478 .. 15181761
1284 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G128000.1

Sequence Viewer

Length: 1191 bp
ATGGAATCGTCCACACTTCTAGCTTTACTCTCCTCCATCTCGCAACTCATCCTCCTCCTCCTCCTTTCTCCCAATTCCACCAATTCATCCAGTTCCTGCGCACATGTTTTCCCAATCGCCCACAGCTTCCTCTCTTCCCACGAAATGGCCGCCACCCTTTCCCTCCTCACCCTTTCAAGGAAACGAAAGCGAACCCATTTCTCAGAACGAGATTCGGAGCCCACTGACCATGACAAGGACCAAGAACTCGGTGGCGGCGACTCGGTCCAACTGGGTTTGACTCGCAGTCCCGACTCGTTCAGAAACTGCTTCAGAATGACCTACTCTACCTTCGAATGGCTCTGTGGTTTACTCGAGCCGCTGCTCGAGTGTCGTGACCCACAATTCGGGGTTTCGGAGCCCGTGGCAAGGTTCTGTGCCAAGCAATTGTGCCGAGTTCTTTGCACCAATTACCGTTTCTGGATTGAATTTCCCAACCCAAATGAGCTTGCCTCTGTATCGGCGGCTTTCGGAAGCCAAACTGGGTTGCCCAATTGCTGTGGTGTGATTGATTGTACAAGGTTTAAGACTGTTAAAAATGGTGGCTTTCACGAGGAGAGCATTGCTGCCCAAATCATGGTCGATTCTTCATCGCGAATTCTCAGCATTGTTGCCGGGTTTCGTGGCAACAAGGGTGATTCAAGAGTATTAAAGTCGTCAACTTTGTACAAGGATATTGAAGCAGGGAGGTTATTGAATTCTCCTCCAGTTAATGTAGATGGGGTGGCTGTAAATCAGTACTTAATTGGAGATGAAGGGTACCCTTTACTTCCTTGGCTTATGGTCCCTTTTGTTGATGCTGCGAAGGGCTCAAATGAAGAGCATTTCAATGCAGCACACAATCTGATGCGCCTTTCGGCGCTTAGGACCATTGTTAGTTTGAAGAGTTGGGGTATTTTGAGCCGGCCAATACAAGAGGAGTTCAAGATGGCAGTTGCTTATATTGGTGCTTGTTCTATACTGCATAATGGTTTGCTAAGGAGGGAGGACTTTTCTGCTATGTGTGATGTGGATGATTACTCTTTGTACGATCAGAGCTCTCAGTATTATAGGGATACTAGTTTGGAGGAGAATTCAATTGAGAGGAAGGCTTCTGTAATACGAACTGCATTGGCCGCAAAAGCAAAAGAGTTTCAAAACACACAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

397

Amino Acids

43.91

Weight (kDa)

6.02

Isoelectric Point (pI)

49.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 100
Acc65I GGTACC 1 cut(s) 798
AccB1I GGYRCC 1 cut(s) 798
AccB7I CCANNNNNTGG 2 cut(s) 145, 616
AccII CGCG 1 cut(s) 634
AciI CCGC 5 cut(s) 150, 255, 359, 503, 1155
AcoI YGGCCR 3 cut(s) 147, 944, 1152
AcsI RAATTY 4 cut(s) 467, 636, 736, 1111
AcuI CTGAAG 1 cut(s) 295
AfaI GTAC 5 cut(s) 556, 707, 779, 800, 1067
AfiI CCNNNNNNNGG 7 cut(s) 145, 177, 235, 336, 386, 408, 616
AflIII ACRYGT 1 cut(s) 103
AhdI GACNNNNNGTC 1 cut(s) 285
AhlI ACTAGT 1 cut(s) 1097
AjuI GAANNNNNNNTTGG 2 cut(s) 440, 472
AluBI AGCT 4 cut(s) 23, 126, 487, 1077
AluI AGCT 4 cut(s) 23, 126, 487, 1077
Alw21I GWGCWC 1 cut(s) 1079
AlwNI CAGNNNCTG 2 cut(s) 96, 306
Ama87I CYCGRG 2 cut(s) 353, 365
AoxI GGCC 3 cut(s) 147, 944, 1152
ApeKI GCWGC 4 cut(s) 361, 605, 839, 872
ApoI RAATTY 4 cut(s) 467, 636, 736, 1111
ArsI GACNNNNNNTTYG 2 cut(s) 368, 400
Asp718I GGTACC 1 cut(s) 798
AspLEI GCGC 3 cut(s) 101, 891, 901
AspS9I GGNCC 4 cut(s) 238, 265, 823, 906
AsuC2I CCSGG 1 cut(s) 655
AsuHPI GGTGA 2 cut(s) 160, 686
AsuII TTCGAA 1 cut(s) 333
AvaI CYCGRG 2 cut(s) 353, 365
AvaII GGWCC 4 cut(s) 238, 265, 823, 906
BanI GGYRCC 1 cut(s) 798
BanII GRGCYC 4 cut(s) 222, 402, 851, 1079
BauI CACGAG 1 cut(s) 590
Bbv12I GWGCWC 1 cut(s) 1079
BbvI GCAGC 4 cut(s) 348, 592, 826, 884
BccI CCATC 3 cut(s) 44, 752, 961
BcgI CGANNNNNNTGC 2 cut(s) 423, 457
BciVI GTATCC 1 cut(s) 1087
BcnI CCSGG 1 cut(s) 655
BcuI ACTAGT 1 cut(s) 1097
BfaI CTAG 2 cut(s) 20, 1098
BfoI RGCGCY 1 cut(s) 902
BfuI GTATCC 1 cut(s) 1087
BisI GCNGC 9 cut(s) 150, 256, 359, 362, 504, 606, 840, 873, 1155
BlsI GCNGC 9 cut(s) 151, 257, 360, 363, 505, 607, 841, 874, 1156
BmcAI AGTACT 1 cut(s) 779
Bme1390I CCNGG 1 cut(s) 655
Bme18I GGWCC 4 cut(s) 238, 265, 823, 906
BmeRI GACNNNNNGTC 1 cut(s) 285
BmeT110I CYCGRG 2 cut(s) 353, 365
BmgT120I GGNCC 4 cut(s) 238, 265, 823, 906
BmiI GGNNCC 4 cut(s) 219, 399, 800, 825
BmrFI CCNGG 1 cut(s) 655
BmrI ACTGGG 2 cut(s) 281, 531
BmsI GCATC 2 cut(s) 826, 876
BmuI ACTGGG 2 cut(s) 281, 531
BplI GAGNNNNNCTC 2 cut(s) 476, 508
BpmI CTGGAG 1 cut(s) 729
Bpu10I CCTNAGC 2 cut(s) 902, 1016
Bpu14I TTCGAA 1 cut(s) 333
BpuMI CCSGG 1 cut(s) 655
BsaJI CCNNGG 2 cut(s) 402, 812
BsaXI ACNNNNNCTCC 2 cut(s) 36, 66
Bsc4I CCNNNNNNNGG 7 cut(s) 145, 177, 235, 336, 386, 408, 616
Bse118I RCCGGY 1 cut(s) 942
Bse1I ACTGG 4 cut(s) 90, 276, 526, 746
Bse3DI GCAATG 1 cut(s) 600
BseDI CCNNGG 2 cut(s) 402, 812
BseGI GGATG 3 cut(s) 48, 86, 1057
BseLI CCNNNNNNNGG 7 cut(s) 145, 177, 235, 336, 386, 408, 616
BseMI GCAATG 1 cut(s) 600
BseMII CTCAG 3 cut(s) 216, 655, 1094
BseNI ACTGG 4 cut(s) 90, 276, 526, 746
BseRI GAGGAG 9 cut(s) 22, 44, 47, 50, 155, 608, 732, 971, 1121
BseXI GCAGC 4 cut(s) 348, 592, 826, 884
Bsh1236I CGCG 1 cut(s) 634
BshFI GGCC 3 cut(s) 149, 946, 1154
BshNI GGYRCC 1 cut(s) 798
BsiHKAI GWGCWC 1 cut(s) 1079
BsiHKCI CYCGRG 2 cut(s) 353, 365
BsiSI CCGG 2 cut(s) 654, 943
BslFI GGGAC 2 cut(s) 273, 809
BslI CCNNNNNNNGG 7 cut(s) 145, 177, 235, 336, 386, 408, 616
BsmFI GGGAC 2 cut(s) 273, 809
BsnI GGCC 3 cut(s) 149, 946, 1154
BsoBI CYCGRG 2 cut(s) 353, 365
Bsp119I TTCGAA 1 cut(s) 333
Bsp1286I GDGCHC 4 cut(s) 222, 402, 851, 1079
Bsp1407I TGTACA 2 cut(s) 554, 705
Bsp143I GATC 1 cut(s) 1069
Bsp68I TCGCGA 1 cut(s) 634
BspACI CCGC 5 cut(s) 150, 255, 359, 503, 1155
BspANI GGCC 3 cut(s) 149, 946, 1154
BspCNI CTCAG 3 cut(s) 215, 654, 1093
BspFNI CGCG 1 cut(s) 634
BspLI GGNNCC 4 cut(s) 219, 399, 800, 825
BspQI GCTCTTC 1 cut(s) 852
BspT104I TTCGAA 1 cut(s) 333
BspT107I GGYRCC 1 cut(s) 798
BsrDI GCAATG 1 cut(s) 600
BsrFI RCCGGY 1 cut(s) 942
BsrGI TGTACA 2 cut(s) 554, 705
BsrI ACTGG 4 cut(s) 90, 276, 526, 746
BssAI RCCGGY 1 cut(s) 942
BssECI CCNNGG 2 cut(s) 402, 812
BssMI GATC 1 cut(s) 1069
BssSI CACGAG 1 cut(s) 590
BssT1I CCWWGG 1 cut(s) 812
Bst2BI CACGAG 1 cut(s) 590
Bst4CI ACNGT 2 cut(s) 455, 571
Bst6I CTCTTC 3 cut(s) 139, 852, 917
BstAUI TGTACA 2 cut(s) 554, 705
BstBI TTCGAA 1 cut(s) 333
BstC8I GCNNGC 2 cut(s) 489, 944
BstDEI CTNAG 5 cut(s) 202, 641, 902, 1016, 1080
BstDSI CCRYGG 1 cut(s) 402
BstF5I GGATG 3 cut(s) 48, 86, 1057
BstFNI CGCG 1 cut(s) 634
BstH2I RGCGCY 1 cut(s) 902
BstHHI GCGC 3 cut(s) 101, 891, 901
BstKTI GATC 1 cut(s) 1072
BstMBI GATC 1 cut(s) 1069
BstMWI GCNNNNNNNGC 2 cut(s) 1154, 1160
BstNSI RCATGY 1 cut(s) 107
BstSCI CCNGG 1 cut(s) 653
BstUI CGCG 1 cut(s) 634
BstV1I GCAGC 4 cut(s) 348, 592, 826, 884
BsuI GTATCC 1 cut(s) 1087
BsuRI GGCC 3 cut(s) 149, 946, 1154
BtgI CCRYGG 1 cut(s) 402
BtgZI GCGATG 1 cut(s) 615
BtsCI GGATG 3 cut(s) 48, 86, 1057
BtsIMutI CAGTG 1 cut(s) 222
BtuMI TCGCGA 1 cut(s) 634
Cac8I GCNNGC 2 cut(s) 489, 944
CaiI CAGNNNCTG 2 cut(s) 96, 306
CfoI GCGC 3 cut(s) 101, 891, 901
Cfr10I RCCGGY 1 cut(s) 942
Cfr13I GGNCC 4 cut(s) 238, 265, 823, 906
Csp6I GTAC 5 cut(s) 555, 706, 778, 799, 1066
CspCI CAANNNNNGTGG 2 cut(s) 520, 555
CviAII CATG 3 cut(s) 104, 230, 616
CviQI GTAC 5 cut(s) 555, 706, 778, 799, 1066
DdeI CTNAG 5 cut(s) 202, 641, 902, 1016, 1080
DpnI GATC 1 cut(s) 1071
DpnII GATC 1 cut(s) 1069
DriI GACNNNNNGTC 1 cut(s) 285
EaeI YGGCCR 3 cut(s) 147, 944, 1152
Eam1104I CTCTTC 3 cut(s) 139, 852, 917
Eam1105I GACNNNNNGTC 1 cut(s) 285
EarI CTCTTC 3 cut(s) 139, 852, 917
Ecl136II GAGCTC 1 cut(s) 1077
Eco130I CCWWGG 1 cut(s) 812
Eco24I GRGCYC 4 cut(s) 222, 402, 851, 1079
Eco47I GGWCC 4 cut(s) 238, 265, 823, 906
Eco53kI GAGCTC 1 cut(s) 1077
Eco57I CTGAAG 1 cut(s) 295
Eco88I CYCGRG 2 cut(s) 353, 365
EcoICRI GAGCTC 1 cut(s) 1077
EcoRI GAATTC 3 cut(s) 636, 736, 1111
EcoT14I CCWWGG 1 cut(s) 812
EcoT38I GRGCYC 4 cut(s) 222, 402, 851, 1079
ErhI CCWWGG 1 cut(s) 812
FaeI CATG 3 cut(s) 107, 233, 619
FaiI YATR 9 cut(s) 105, 231, 617, 821, 981, 998, 1005, 1040, 1089
FaqI GGGAC 2 cut(s) 273, 809
FatI CATG 3 cut(s) 103, 229, 615
Fnu4HI GCNGC 9 cut(s) 150, 256, 359, 362, 504, 606, 840, 873, 1155
FokI GGATG 3 cut(s) 35, 73, 1064
FriOI GRGCYC 4 cut(s) 222, 402, 851, 1079
Fsp4HI GCNGC 9 cut(s) 150, 256, 359, 362, 504, 606, 840, 873, 1155
FspBI CTAG 2 cut(s) 20, 1098
FspI TGCGCA 1 cut(s) 100
GlaI GCGC 3 cut(s) 100, 890, 900
GluI GCNGC 9 cut(s) 150, 256, 359, 362, 504, 606, 840, 873, 1155
GsuI CTGGAG 1 cut(s) 729
HaeII RGCGCY 1 cut(s) 902
HaeIII GGCC 3 cut(s) 149, 946, 1154
HapII CCGG 2 cut(s) 654, 943
HhaI GCGC 3 cut(s) 101, 891, 901
Hin1II CATG 3 cut(s) 107, 233, 619
Hin6I GCGC 3 cut(s) 99, 889, 899
HinP1I GCGC 3 cut(s) 99, 889, 899
HincII GTYRAC 1 cut(s) 699
HindII GTYRAC 1 cut(s) 699
HinfI GANTC 7 cut(s) 5, 212, 260, 280, 293, 623, 677
HpaII CCGG 2 cut(s) 654, 943
HphI GGTGA 2 cut(s) 160, 686
Hpy166II GTNNAC 3 cut(s) 12, 350, 699
Hpy188I TCNGA 8 cut(s) 205, 217, 302, 314, 397, 512, 885, 1074
Hpy188III TCNNGA 7 cut(s) 290, 374, 460, 590, 633, 681, 964
Hpy8I GTNNAC 3 cut(s) 12, 350, 699
HpyAV CCTTC 4 cut(s) 340, 788, 838, 1120
HpyCH4III ACNGT 2 cut(s) 455, 571
HpyCH4V TGCA 4 cut(s) 444, 872, 1003, 1148
HpyF10VI GCNNNNNNNGC 2 cut(s) 1154, 1160
HpyF3I CTNAG 5 cut(s) 202, 641, 902, 1016, 1080
Hsp92II CATG 3 cut(s) 107, 233, 619
HspAI GCGC 3 cut(s) 99, 889, 899
KpnI GGTACC 1 cut(s) 802
KroI GCCGGC 1 cut(s) 942
KroNI GCCGGC 1 cut(s) 944
Kzo9I GATC 1 cut(s) 1069
LguI GCTCTTC 1 cut(s) 852
LmnI GCTCC 2 cut(s) 217, 397
LpnPI CCDG 9 cut(s) 103, 109, 257, 445, 507, 667, 708, 759, 956
Lsp1109I GCAGC 4 cut(s) 348, 592, 826, 884
LweI GCATC 2 cut(s) 826, 876
MaeI CTAG 2 cut(s) 20, 1098
MaeIII GTNAC 1 cut(s) 374
MalI GATC 1 cut(s) 1071
MboI GATC 1 cut(s) 1069
MboII GAAGA 4 cut(s) 126, 618, 869, 934
MfeI CAATTG 3 cut(s) 425, 532, 1116
MhlI GDGCHC 4 cut(s) 222, 402, 851, 1079
MlyI GAGTC 3 cut(s) 254, 274, 287
MmeI TCCRAC 1 cut(s) 292
MroNI GCCGGC 1 cut(s) 942
MseI TTAA 5 cut(s) 564, 573, 689, 750, 782
MslI CAYNNNNRTG 1 cut(s) 867
MspA1I CMGCKG 1 cut(s) 361
MspI CCGG 2 cut(s) 654, 943
MspR9I CCNGG 1 cut(s) 655
MunI CAATTG 3 cut(s) 425, 532, 1116
MvnI CGCG 1 cut(s) 634
MwoI GCNNNNNNNGC 2 cut(s) 1154, 1160
NaeI GCCGGC 1 cut(s) 944
NciI CCSGG 1 cut(s) 655
NdeII GATC 1 cut(s) 1069
NgoMIV GCCGGC 1 cut(s) 942
NlaIII CATG 3 cut(s) 107, 233, 619
NlaIV GGNNCC 4 cut(s) 219, 399, 800, 825
NmeAIII GCCGAG 1 cut(s) 458
NmuCI GTSAC 1 cut(s) 374
NruI TCGCGA 1 cut(s) 634
NsbI TGCGCA 1 cut(s) 100
NspI RCATGY 1 cut(s) 107
NspV TTCGAA 1 cut(s) 333
PaeR7I CTCGAG 2 cut(s) 353, 365
PciI ACATGT 1 cut(s) 103
PciSI GCTCTTC 1 cut(s) 852
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PdiI GCCGGC 1 cut(s) 944
PfeI GAWTC 4 cut(s) 5, 212, 623, 677
PflFI GACNNNGTC 1 cut(s) 263
PflMI CCANNNNNTGG 2 cut(s) 145, 616
PkrI GCNGC 9 cut(s) 151, 257, 360, 363, 505, 607, 841, 874, 1156
PleI GAGTC 3 cut(s) 254, 274, 287
PpsI GAGTC 3 cut(s) 254, 274, 287
PscI ACATGT 1 cut(s) 103
Psp124BI GAGCTC 1 cut(s) 1079
PspN4I GGNNCC 4 cut(s) 219, 399, 800, 825
PspPI GGNCC 4 cut(s) 238, 265, 823, 906
PspXI VCTCGAGB 2 cut(s) 353, 365
PstNI CAGNNNCTG 2 cut(s) 96, 306
PsyI GACNNNGTC 1 cut(s) 263
RruI TCGCGA 1 cut(s) 634
RsaI GTAC 5 cut(s) 556, 707, 779, 800, 1067
RsaNI GTAC 5 cut(s) 555, 706, 778, 799, 1066
RseI CAYNNNNRTG 1 cut(s) 867
SacI GAGCTC 1 cut(s) 1079
SapI GCTCTTC 1 cut(s) 852
SaqAI TTAA 5 cut(s) 564, 573, 689, 750, 782
SatI GCNGC 9 cut(s) 150, 256, 359, 362, 504, 606, 840, 873, 1155
Sau3AI GATC 1 cut(s) 1069
Sau96I GGNCC 4 cut(s) 238, 265, 823, 906
ScaI AGTACT 1 cut(s) 779
SchI GAGTC 3 cut(s) 254, 274, 287
ScrFI CCNGG 1 cut(s) 655
SduI GDGCHC 4 cut(s) 222, 402, 851, 1079
SetI ASST 9 cut(s) 25, 128, 323, 332, 413, 489, 563, 731, 1079
SfaNI GCATC 2 cut(s) 826, 876
Sfr274I CTCGAG 2 cut(s) 353, 365
SfuI TTCGAA 1 cut(s) 333
SinI GGWCC 4 cut(s) 238, 265, 823, 906
SlaI CTCGAG 2 cut(s) 353, 365
SmiMI CAYNNNNRTG 1 cut(s) 867
SmlI CTYRAG 2 cut(s) 353, 365
SmoI CTYRAG 2 cut(s) 353, 365
SpeI ACTAGT 1 cut(s) 1097
SsiI CCGC 5 cut(s) 150, 255, 359, 503, 1155
SspMI CTAG 2 cut(s) 20, 1098
SstI GAGCTC 1 cut(s) 1079
StyD4I CCNGG 1 cut(s) 653
StyI CCWWGG 1 cut(s) 812
TaaI ACNGT 2 cut(s) 455, 571
TaqI TCGA 4 cut(s) 333, 354, 366, 621
TaqII GACCGA 1 cut(s) 253
TatI WGTACW 3 cut(s) 554, 705, 777
TauI GCSGC 5 cut(s) 152, 258, 361, 506, 1157
TfiI GAWTC 4 cut(s) 5, 212, 623, 677
Tru1I TTAA 5 cut(s) 564, 573, 689, 750, 782
Tru9I TTAA 5 cut(s) 564, 573, 689, 750, 782
TscAI CASTG 1 cut(s) 229
TseFI GTSAC 1 cut(s) 374
TseI GCWGC 4 cut(s) 361, 605, 839, 872
Tsp45I GTSAC 1 cut(s) 374
TspDTI ATGAA 4 cut(s) 75, 618, 807, 870
TspRI CASTG 1 cut(s) 229
Tth111I GACNNNGTC 1 cut(s) 263
Van91I CCANNNNNTGG 2 cut(s) 145, 616
VpaK11BI GGWCC 4 cut(s) 238, 265, 823, 906
XapI RAATTY 4 cut(s) 467, 636, 736, 1111
XceI RCATGY 1 cut(s) 107
XcmI CCANNNNNNNNNTGG 1 cut(s) 248
XhoI CTCGAG 2 cut(s) 353, 365
XspI CTAG 2 cut(s) 20, 1098
ZrmI AGTACT 1 cut(s) 779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.