FvH4_2g06510

Carboxylesterase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0006911
Physical Location & Seq
Reverse (-)
5414680 .. 5415141
462 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g06510.t1

Sequence Viewer

Length: 462 bp
ATGGGGATTTTGATAGACTTTTTTTTCTATGGGGACAACGGTGGAGTGAACTTGTGTCACAATTTGGCCGTTCGGATATGTTCTAATGAGTTACCCGAGGATAAAATTGTGGGAATTGTTATGGTGCGCCCGTTCTTTGGCGGGTTAGAGGAGGACGACAAAATGTGGCTATACATGTGTCCGGAGAACGGAGGGGTGAAAGACAGGAGGCTGAGGCCGCCGGCGGAGGATCTTGCGAAGCTTGGGTGCGAGAGGGTGTTGATATTTTTTGCGGAGAAGGATCATCTGAGAGAGGTAGGGCAGTGGTATTGTGATGAGCTGAAGAAGAGTGAGTGGGGTGGGAGTGTTGAAGTGGTGGAGCATAAGGATGTTGCCCATGAGTTTCACTTGTTGAGCGAACCTGATGGTGAGAGAGCTCTTGACTTGATCAAGAAAATTGGTTGCTTTATCAAACAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

154

Amino Acids

17.51

Weight (kDa)

5.14

Isoelectric Point (pI)

42.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_3 PF07859 7 - 129 3e-09 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 181
AciI CCGC 4 cut(s) 141, 218, 224, 272
AclWI GGATC 2 cut(s) 237, 288
AcoI YGGCCR 1 cut(s) 66
AcuI CTGAAG 1 cut(s) 341
AfiI CCNNNNNNNGG 2 cut(s) 137, 188
AflIII ACRYGT 1 cut(s) 174
AgsI TTSAA 1 cut(s) 350
AluBI AGCT 3 cut(s) 241, 319, 416
AluI AGCT 3 cut(s) 241, 319, 416
Alw21I GWGCWC 1 cut(s) 418
AlwI GGATC 2 cut(s) 237, 288
Ama87I CYCGRG 1 cut(s) 95
Aor13HI TCCGGA 1 cut(s) 181
AoxI GGCC 2 cut(s) 66, 215
AspLEI GCGC 1 cut(s) 129
AsuHPI GGTGA 2 cut(s) 208, 419
AvaI CYCGRG 1 cut(s) 95
BanII GRGCYC 1 cut(s) 418
Bbv12I GWGCWC 1 cut(s) 418
BbvCI CCTCAGC 1 cut(s) 212
BccI CCATC 1 cut(s) 398
BceAI ACGGC 1 cut(s) 53
BclI TGATCA 1 cut(s) 426
BisI GCNGC 1 cut(s) 218
BlsI GCNGC 1 cut(s) 219
BmeT110I CYCGRG 1 cut(s) 95
Bpu10I CCTNAGC 1 cut(s) 212
BsaJI CCNNGG 1 cut(s) 96
BsaWI WCCGGW 1 cut(s) 181
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 188
Bse118I RCCGGY 1 cut(s) 220
BseAI TCCGGA 1 cut(s) 181
BseDI CCNNGG 1 cut(s) 96
BseGI GGATG 1 cut(s) 373
BseLI CCNNNNNNNGG 2 cut(s) 137, 188
BseMII CTCAG 2 cut(s) 203, 278
BseRI GAGGAG 1 cut(s) 164
BshFI GGCC 2 cut(s) 68, 217
BsiHKAI GWGCWC 1 cut(s) 418
BsiHKCI CYCGRG 1 cut(s) 95
BsiSI CCGG 2 cut(s) 182, 221
BslFI GGGAC 1 cut(s) 47
BslI CCNNNNNNNGG 2 cut(s) 137, 188
BsmFI GGGAC 1 cut(s) 47
BsnI GGCC 2 cut(s) 68, 217
BsoBI CYCGRG 1 cut(s) 95
Bsp1286I GDGCHC 1 cut(s) 418
Bsp13I TCCGGA 1 cut(s) 181
Bsp143I GATC 3 cut(s) 229, 280, 426
BspACI CCGC 4 cut(s) 141, 218, 224, 272
BspANI GGCC 2 cut(s) 68, 217
BspCNI CTCAG 2 cut(s) 204, 279
BspEI TCCGGA 1 cut(s) 181
BspPI GGATC 2 cut(s) 237, 288
BsrFI RCCGGY 1 cut(s) 220
BssAI RCCGGY 1 cut(s) 220
BssECI CCNNGG 1 cut(s) 96
BssMI GATC 3 cut(s) 229, 280, 426
Bst4CI ACNGT 1 cut(s) 41
Bst6I CTCTTC 1 cut(s) 320
BstC8I GCNNGC 1 cut(s) 222
BstDEI CTNAG 2 cut(s) 212, 287
BstF5I GGATG 1 cut(s) 373
BstHHI GCGC 1 cut(s) 129
BstKTI GATC 3 cut(s) 232, 283, 429
BstMBI GATC 3 cut(s) 229, 280, 426
BstMWI GCNNNNNNNGC 1 cut(s) 217
BstNSI RCATGY 1 cut(s) 178
BstX2I RGATCY 1 cut(s) 229
BstYI RGATCY 1 cut(s) 229
BsuRI GGCC 2 cut(s) 68, 217
BtsCI GGATG 1 cut(s) 373
BtsI GCAGTG 1 cut(s) 308
BtsIMutI CAGTG 1 cut(s) 308
Cac8I GCNNGC 1 cut(s) 222
CfoI GCGC 1 cut(s) 129
Cfr10I RCCGGY 1 cut(s) 220
CviAII CATG 2 cut(s) 175, 377
CviJI RGCY 7 cut(s) 68, 169, 211, 217, 241, 319, 416
CviKI_1 RGCY 7 cut(s) 68, 169, 211, 217, 241, 319, 416
DdeI CTNAG 2 cut(s) 212, 287
DpnI GATC 3 cut(s) 231, 282, 428
DpnII GATC 3 cut(s) 229, 280, 426
EaeI YGGCCR 1 cut(s) 66
Eam1104I CTCTTC 1 cut(s) 320
EarI CTCTTC 1 cut(s) 320
EciI GGCGGA 1 cut(s) 239
Ecl136II GAGCTC 1 cut(s) 416
Eco24I GRGCYC 1 cut(s) 418
Eco53kI GAGCTC 1 cut(s) 416
Eco57I CTGAAG 1 cut(s) 341
Eco88I CYCGRG 1 cut(s) 95
EcoICRI GAGCTC 1 cut(s) 416
EcoT38I GRGCYC 1 cut(s) 418
FaeI CATG 2 cut(s) 178, 380
FaiI YATR 7 cut(s) 30, 79, 122, 172, 176, 363, 378
FaqI GGGAC 1 cut(s) 47
FatI CATG 2 cut(s) 174, 376
FauI CCCGC 1 cut(s) 134
FbaI TGATCA 1 cut(s) 426
Fnu4HI GCNGC 1 cut(s) 218
FokI GGATG 1 cut(s) 380
FriOI GRGCYC 1 cut(s) 418
Fsp4HI GCNGC 1 cut(s) 218
GlaI GCGC 1 cut(s) 128
GluI GCNGC 1 cut(s) 218
HaeIII GGCC 2 cut(s) 68, 217
HapII CCGG 2 cut(s) 182, 221
HhaI GCGC 1 cut(s) 129
Hin1II CATG 2 cut(s) 178, 380
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HindIII AAGCTT 1 cut(s) 239
HpaII CCGG 2 cut(s) 182, 221
HphI GGTGA 2 cut(s) 208, 419
Hpy166II GTNNAC 1 cut(s) 49
Hpy188I TCNGA 2 cut(s) 75, 288
Hpy188III TCNNGA 3 cut(s) 182, 419, 430
Hpy8I GTNNAC 1 cut(s) 49
HpyAV CCTTC 1 cut(s) 271
HpyCH4III ACNGT 1 cut(s) 41
HpyF10VI GCNNNNNNNGC 1 cut(s) 217
HpyF3I CTNAG 2 cut(s) 212, 287
Hsp92II CATG 2 cut(s) 178, 380
HspAI GCGC 1 cut(s) 127
Kpn2I TCCGGA 1 cut(s) 181
KroI GCCGGC 1 cut(s) 220
KroNI GCCGGC 1 cut(s) 222
Ksp22I TGATCA 1 cut(s) 426
Kzo9I GATC 3 cut(s) 229, 280, 426
LmnI GCTCC 1 cut(s) 358
LpnPI CCDG 4 cut(s) 190, 195, 234, 414
MaeIII GTNAC 2 cut(s) 56, 90
MalI GATC 3 cut(s) 231, 282, 428
MboI GATC 3 cut(s) 229, 280, 426
MboII GAAGA 2 cut(s) 334, 337
MflI RGATCY 1 cut(s) 229
MhlI GDGCHC 1 cut(s) 418
MluCI AATT 4 cut(s) 61, 105, 114, 435
MnlI CCTC 9 cut(s) 91, 142, 145, 185, 201, 207, 220, 246, 286
MreI CGCCGGCG 1 cut(s) 220
MroI TCCGGA 1 cut(s) 181
MroNI GCCGGC 1 cut(s) 220
MslI CAYNNNNRTG 1 cut(s) 366
MspI CCGG 2 cut(s) 182, 221
MwoI GCNNNNNNNGC 1 cut(s) 217
NaeI GCCGGC 1 cut(s) 222
NdeII GATC 3 cut(s) 229, 280, 426
NgoMIV GCCGGC 1 cut(s) 220
NlaIII CATG 2 cut(s) 178, 380
NmuCI GTSAC 1 cut(s) 56
NspI RCATGY 1 cut(s) 178
PciI ACATGT 1 cut(s) 174
PdiI GCCGGC 1 cut(s) 222
PkrI GCNGC 1 cut(s) 219
PscI ACATGT 1 cut(s) 174
Psp124BI GAGCTC 1 cut(s) 418
PsuI RGATCY 1 cut(s) 229
RseI CAYNNNNRTG 1 cut(s) 366
SacI GAGCTC 1 cut(s) 418
SatI GCNGC 1 cut(s) 218
Sau3AI GATC 3 cut(s) 229, 280, 426
SduI GDGCHC 1 cut(s) 418
SetI ASST 5 cut(s) 243, 297, 321, 403, 418
SgrAI CRCCGGYG 1 cut(s) 220
SmiMI CAYNNNNRTG 1 cut(s) 366
Sse9I AATT 4 cut(s) 61, 105, 114, 435
SsiI CCGC 4 cut(s) 141, 218, 224, 272
SstI GAGCTC 1 cut(s) 418
TaaI ACNGT 1 cut(s) 41
TasI AATT 4 cut(s) 61, 105, 114, 435
TauI GCSGC 1 cut(s) 220
TscAI CASTG 1 cut(s) 308
TseFI GTSAC 1 cut(s) 56
Tsp45I GTSAC 1 cut(s) 56
TspGWI ACGGA 1 cut(s) 204
TspRI CASTG 1 cut(s) 308
XceI RCATGY 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.