Rorug05G0581200

Carboxylesterase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0006911
Physical Location & Seq
Reverse (-)
77864517 .. 77870948
6432 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0581200.1

Sequence Viewer

Length: 3453 bp
ATGACCACTCAATTTGAGGCCTCTTCCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTCTTCCCCTTCCCCTTCTGCTACCATTCAGTCATGGACATATCATGTCTTTCTGAGTTTTAGAGGAGAGGATACGCGCAACACTTTTACAGGCCATTTGTACAGAAATTTGGTTCAGAAGGGAATCAAAACCTTCATAGACGATGGCCTTCCAAGAGGAGAAGAAATATCAAGCGCTCTTTTTAAAGCAATTGAAGAGTCAAAAATTTCTGTCATTGTGTTCTCTGAAAATTATGCTTCTTCAAAATGGTGCTTAGAAGAACTGGTCAAGATCATTCAGTGCAAAGAATCAAAGAACCAAATGGTATACCCTGTTTTTTACAAGGTAGATCCATCAGATGTACGAAAGCAACGCGGTAGTTTTGGTCAGGCACTTGCTCACCATGATAATTCAGAGAAGGTGGTAGGATGGAAGACGGCTCTTACAAAAGCAGGAAATCTTTCTGGTTGGCATATCTCACAAGAAGGGAATGAATCTAAATTTATTCATGAAATTGTTCAAGAGATTTCACAACAAGATTTAAATTTTACATATTTAAATGGGGACAAATACTTAGTTGGAATAAAGTCTCGTTTGAAAGATGTGAATAAACTTTTATGTGTTGGAGAAAGTGATGTACAAATGGTGGGGATATGGGGCATTGGTGGATTAGGTAAAACAACCATTGCCAAGGCTGTTTACAATTCGATTGCCCGTGAATTTGAAGGCTGTTGTTTTTTGGAAAATGTTAGAGAACGATTGTTGAAATATGGAGACCTGCAAAAACTACAAAAGTTTCTTCTCTCTGAGACTCTAGGGTTGAAAGAAGTAAAGGTAAATAGTCTTGACAAAGGAAGCACTATGATAAAAGAAAGTTTAAGCCATAAGAGAGTTCTCTTAGTTCTTGATGATGTGAATGATGTCAACCAGTTAAAAAGTTTAGCTGGAAAGTCTAATTGGTTTGGTCCTGGTAGTATAATAATCATAACAACACGGGATAAACATTTGCTACATGATGTTAATCACATATACCAAGTGGAGAAGTTAAGTCATCCTGAAGCTCTTGAGCTCTTCAACTTTAATGCATTCAAAGGAAAAGGACATATGGGTGATTACGTTGAACTCATAGATAATGTAATACGCTATGCTGAAGGGCTTCCTCTAGTTGTGGAAAATATGGGTTCATATCTTCATGGCAGAAGTATAGATACATGGAAAGATGCATTAGAGTTTTACAAAAGAAATCCTAAACTTCGACATTTTCTCAAATTAAGTTATGATTCACTTGAACCTTCGATTAAGGAAATTTTTCTTCATATCGCATGTTTCTTCAATGGTGAAAATAAGAACTATGTGATGGACATATTAGCAGGATGTGACCTACCTAAACATGGGATTGAAATACTCATAGAAAAGTCCCTCATAAGCATTACTGAAACAAATGGTATTTGGATGCATGGCATGCTGGAAGAAATGGGCAAAGAAATTGTTTGTCAAGAGTCATCAGAGCCAGGAGAACGTAGCAGACTGTGGTCGTACGAAGATGTTTACCACGTTTTTGTGCACAACACTGGTACAAACAAAGTTAAAGGGGTCATGGTTAAGAAGGGCAAACCGGACAAACCAAATCAACAAATACCCTTGAATAGTGAAAGCTTCTTAAAGTTAAACAGTCTTGAAATTCTTATAATTTCTGATGATATATTCAGTGGAGATTGCGTGGATTATCTCCCCATTGAGTTGATTCTCCTTGATTGGGTGGTTTGTCCATTACAATATTTTCCATCAAATTTTTATCCTGAGAAACTTGTTGTACTCAAGATAAATTTCACCTTAATGTCACCGAGGAGGGCACTTGAGACAGGTTTTGAACATATAATGTCTTGGATCTGCACATCACCACAGATTATGCAAAATTTGAAATCACTCGATTTGGGAAATTGCGATGCTGTAACAACTTTGTCCGACTTCTCTAGATTCCCAAACTTAGAGGAGTTAAAAATTAGAGGCAGTGGAAAAGTTGAGAAGTTTGAAATTGTGGAAGAGATGAAATCCTTAAAAAGGCTGGATCTAAGCGGTACTGGAATCAAAGAATTACCTTCATTAGCAATCAGATATCTCATTAATCTGGAACAATTAACTTTATCAGGTTGCAAGAAACTTAAACATGTGCCATGCAACATTTTTGAGTTACAACATCTACAGCTTCTTGATCTCTCGGGTTGTGAAAAGCTGGTTACATTTCCATCAAAGTCAGAGATGAAATCCTTAAAAAGGCTGGATCTAAGCGGTACTGCAATCAAAGATTTACCTTCATCAGCAATCAGATATGTCATTAATCTGGAACAATTAACTTTATCAGGTTGCAAGAAACTTGAACATGTGCCGTGCAACATTTTTGAGTTACAACATCTAGAGCTTCTTGATCTCTCGGGTTGTGAAAAGCTGGTTACATTTCCAACAAAGTCAGAGTTTTCAACTGAATCGACCAGTTTAGAGGACAAGCACTATGCCCCGTTATTCGTCAATTTGAGCAGTTGCTACGATCTTGCAGAAATTGTAGAATTTCCGCGAGAAATATATGGCGTAAATGCGAGCTGCTGCTCTGTATTGAAAAGAATTTCAAAATTGTCAAACATTTTGGAAGGTAAAGAATCAAAAATGACTCGGATGAATTTGTTTCATTGCGGTAAACTGTACCGCACCCTGGCTCGTGATGTAGCAAAGGTGAAAAAGAATTTACCGGATAATTCCAGGTTAACGGCTCTGTTGTCTCTCTTCTTCTCATGTCGGCAATCTGAGTATCAGGTCTTGTTTCCTGGAAGTATTAGGAGCTACGAACGGCTATTCGGGAAAGTGGAATCTAGTTGGAGATTGCCCGAAATTGGACTTCCACAGTGGTTCACCTGCCGCATGTATATCAGTAACAAAGGACTTCAAGTATGCAACTTTGGAATTGATTTTCCTGGAAATTTCAAATGGGAGGACAAAGGATTGGCTTTCTCTGCTTTAACTATTGATAATGAAAATTTGTATACGAAAGCATATATGAGAGTACTCAAAATCTACATTAATGATGTACGCATCATTGACGCATCCGAAAAGCCGGAAGACAGTAGGTCGGGATCGTTCTATGATTTAGTATGGTTGTACTATGTGCCATTCGATACTATAATAAAACGGCTGGGTGAGAGTGGGTTACCGCCGCCTTCCATGTGTCTGGTTAAGTTCGAATTTGAATATAAAACACTCGATGGCGGTGAGGACATTAACGGCTGGATTGGAAGCTGCGGGGTCCACGTAGTAATGCCAGAGGATGAAGGCCCAGTTGTAAATGGGATTGTACGTCCATCAACTTTGATAATGGGGACTAACTCCTACCCGGGGGACGATGCTGAAGATCCACCAACTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

1150

Amino Acids

130.05

Weight (kDa)

6.14

Isoelectric Point (pI)

42.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 33 - 197 7.4e-58 TIR domain
TIR_2 PF13676 35 - 130 3.3e-15 TIR domain
NB-ARC PF00931 213 - 377 4.1e-19 NB-ARC domain
WHD_ROQ1 PF23282 446 - 513 1e-15 Disease resistance protein Roq1-like, winged-helix domain
LRR_RPS2 PF23247 646 - 772 2.2e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_14 PF23598 653 - 795 5.5e-08 Leucine-rich repeat region
LRR_13 PF23286 673 - 883 1.3e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_Zer-1 PF25013 691 - 783 2.3e-06 Zer-1-like, Leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1724
AarI CACCTGC 1 cut(s) 2951
Acc36I ACCTGC 2 cut(s) 822, 2951
AccI GTMKAC 2 cut(s) 363, 3071
AccII CGCG 3 cut(s) 133, 411, 2608
AclWI GGATC 6 cut(s) 380, 1931, 2112, 2325, 3169, 3431
AcuI CTGAAG 3 cut(s) 1113, 1206, 3453
AfeI AGCGCT 1 cut(s) 232
AfiI CCNNNNNNNGG 7 cut(s) 1427, 1792, 2097, 2310, 2743, 2921, 3420
AflIII ACRYGT 2 cut(s) 2203, 2416
AhdI GACNNNNNGTC 1 cut(s) 3154
AjnI CCWGG 6 cut(s) 1003, 1546, 2742, 2789, 2854, 3001
AjuI GAANNNNNNNTTGG 2 cut(s) 976, 1008
Alw21I GWGCWC 2 cut(s) 1107, 1602
Alw26I GTCTC 5 cut(s) 630, 804, 839, 1889, 2814
Alw44I GTGCAC 1 cut(s) 1598
AlwI GGATC 6 cut(s) 380, 1931, 2112, 2325, 3169, 3431
Ama87I CYCGRG 3 cut(s) 2254, 2467, 3418
Aor51HI AGCGCT 1 cut(s) 232
AoxI GGCC 4 cut(s) 18, 148, 202, 3358
ApaLI GTGCAC 1 cut(s) 1598
ApeKI GCWGC 3 cut(s) 2634, 2637, 3324
AseI ATTAAT 3 cut(s) 2160, 2373, 3108
Asp700I GAANNNNTTC 4 cut(s) 496, 552, 1191, 1344
AspLEI GCGC 2 cut(s) 135, 233
AspS9I GGNCC 3 cut(s) 1001, 3331, 3359
AsuC2I CCSGG 2 cut(s) 3419, 3420
AsuII TTCGAA 1 cut(s) 3267
AvaI CYCGRG 3 cut(s) 2254, 2467, 3418
AvaII GGWCC 2 cut(s) 1001, 3331
BaeGI GKGCMC 2 cut(s) 1602, 1891
BanII GRGCYC 1 cut(s) 1107
BarI GAAGNNNNNNTAC 2 cut(s) 1228, 1260
BauI CACGAG 1 cut(s) 2748
BbsI GAAGAC 2 cut(s) 476, 3153
Bbv12I GWGCWC 2 cut(s) 1107, 1602
BbvI GCAGC 3 cut(s) 2621, 2624, 3311
BccI CCATC 8 cut(s) 194, 397, 459, 1387, 1828, 2290, 3284, 3394
BceAI ACGGC 6 cut(s) 489, 2407, 2814, 2894, 3233, 3325
BciT130I CCWGG 6 cut(s) 1005, 1548, 2744, 2791, 2856, 3003
BciVI GTATCC 1 cut(s) 121
BcnI CCSGG 2 cut(s) 3419, 3420
BcoDI GTCTC 5 cut(s) 630, 804, 839, 1889, 2814
BfaI CTAG 5 cut(s) 851, 1199, 2010, 2450, 2901
BfmI CTRYAG 1 cut(s) 2237
BfoI RGCGCY 1 cut(s) 234
BfuAI ACCTGC 2 cut(s) 822, 2951
BfuI GTATCC 1 cut(s) 121
BisI GCNGC 5 cut(s) 2635, 2638, 2947, 3242, 3325
BlsI GCNGC 5 cut(s) 2636, 2639, 2948, 3243, 3326
BmcAI AGTACT 1 cut(s) 3093
Bme1390I CCNGG 8 cut(s) 1005, 1548, 2744, 2791, 2856, 3003, 3419, 3420
Bme18I GGWCC 2 cut(s) 1001, 3331
BmeRI GACNNNNNGTC 1 cut(s) 3154
BmeT110I CYCGRG 3 cut(s) 2254, 2467, 3418
BmgT120I GGNCC 3 cut(s) 1001, 3331, 3359
BmiI GGNNCC 1 cut(s) 3332
BmrFI CCNGG 8 cut(s) 1005, 1548, 2744, 2791, 2856, 3003, 3419, 3420
BmrI ACTGGG 1 cut(s) 3356
BmsI GCATC 6 cut(s) 1246, 1479, 1972, 3129, 3140, 3418
BmuI ACTGGG 1 cut(s) 3356
BoxI GACNNNNGTC 1 cut(s) 1567
BpiI GAAGAC 2 cut(s) 476, 3153
Bpu14I TTCGAA 1 cut(s) 3267
BpuEI CTTGAG 3 cut(s) 1121, 1838, 1913
BpuMI CCSGG 2 cut(s) 3419, 3420
BsaAI YACGTR 1 cut(s) 3337
BsaBI GATNNNNATC 1 cut(s) 1056
BsaI GGTCTC 1 cut(s) 804
BsaJI CCNNGG 5 cut(s) 726, 1880, 2742, 3418, 3419
BsaWI WCCGGW 2 cut(s) 1651, 2779
BsaXI ACNNNNNCTCC 2 cut(s) 1740, 1770
Bsc4I CCNNNNNNNGG 7 cut(s) 1427, 1792, 2097, 2310, 2743, 2921, 3420
Bse1I ACTGG 6 cut(s) 324, 964, 1612, 2122, 2526, 3362
Bse3DI GCAATG 2 cut(s) 720, 2719
Bse8I GATNNNNATC 1 cut(s) 1056
BseBI CCWGG 6 cut(s) 1005, 1548, 2744, 2791, 2856, 3003
BseDI CCNNGG 5 cut(s) 726, 1880, 2742, 3418, 3419
BseGI GGATG 7 cut(s) 470, 1087, 1415, 1494, 2712, 3131, 3358
BseJI GATNNNNATC 1 cut(s) 1056
BseLI CCNNNNNNNGG 7 cut(s) 1427, 1792, 2097, 2310, 2743, 2921, 3420
BseMI GCAATG 2 cut(s) 720, 2719
BseMII CTCAG 4 cut(s) 101, 834, 1827, 2826
BseNI ACTGG 6 cut(s) 324, 964, 1612, 2122, 2526, 3362
BseRI GAGGAG 4 cut(s) 135, 228, 1897, 2042
BseSI GKGCMC 2 cut(s) 1602, 1891
BseXI GCAGC 3 cut(s) 2621, 2624, 3311
BseYI CCCAGC 1 cut(s) 3220
BsgI GTGCAG 1 cut(s) 1912
Bsh1236I CGCG 3 cut(s) 133, 411, 2608
BshFI GGCC 4 cut(s) 20, 150, 204, 3360
BsiHKAI GWGCWC 2 cut(s) 1107, 1602
BsiHKCI CYCGRG 3 cut(s) 2254, 2467, 3418
BsiSI CCGG 4 cut(s) 1652, 2780, 3143, 3419
BsiWI CGTACG 1 cut(s) 1572
BslFI GGGAC 4 cut(s) 614, 1438, 3418, 3437
BslI CCNNNNNNNGG 7 cut(s) 1427, 1792, 2097, 2310, 2743, 2921, 3420
BsmAI GTCTC 5 cut(s) 630, 804, 839, 1889, 2814
BsmFI GGGAC 4 cut(s) 614, 1438, 3418, 3437
BsmI GAATGC 1 cut(s) 1121
BsnI GGCC 4 cut(s) 20, 150, 204, 3360
Bso31I GGTCTC 1 cut(s) 804
BsoBI CYCGRG 3 cut(s) 2254, 2467, 3418
Bsp119I TTCGAA 1 cut(s) 3267
Bsp1286I GDGCHC 3 cut(s) 1107, 1602, 1891
Bsp1407I TGTACA 2 cut(s) 156, 673
BspANI GGCC 4 cut(s) 20, 150, 204, 3360
BspCNI CTCAG 4 cut(s) 102, 835, 1828, 2827
BspFNI CGCG 3 cut(s) 133, 411, 2608
BspHI TCATGA 1 cut(s) 544
BspLI GGNNCC 1 cut(s) 3332
BspMI ACCTGC 2 cut(s) 822, 2951
BspPI GGATC 6 cut(s) 380, 1931, 2112, 2325, 3169, 3431
BspQI GCTCTTC 1 cut(s) 1112
BspT104I TTCGAA 1 cut(s) 3267
BspTNI GGTCTC 1 cut(s) 804
BsrDI GCAATG 2 cut(s) 720, 2719
BsrGI TGTACA 2 cut(s) 156, 673
BsrI ACTGG 6 cut(s) 324, 964, 1612, 2122, 2526, 3362
BssECI CCNNGG 5 cut(s) 726, 1880, 2742, 3418, 3419
BssNAI GTATAC 2 cut(s) 364, 3072
BssSI CACGAG 1 cut(s) 2748
BssT1I CCWWGG 1 cut(s) 726
Bst1107I GTATAC 2 cut(s) 364, 3072
Bst2BI CACGAG 1 cut(s) 2748
Bst2UI CCWGG 6 cut(s) 1005, 1548, 2744, 2791, 2856, 3003
Bst4CI ACNGT 6 cut(s) 1566, 1709, 2733, 2934, 3152, 3448
Bst6I CTCTTC 7 cut(s) 28, 34, 64, 246, 1112, 2073, 2819
BstAPI GCANNNNNTGC 1 cut(s) 1498
BstAUI TGTACA 2 cut(s) 156, 673
BstBAI YACGTR 1 cut(s) 3337
BstBI TTCGAA 1 cut(s) 3267
BstC8I GCNNGC 2 cut(s) 1499, 2632
BstEII GGTNACC 1 cut(s) 3234
BstENI CCTNNNNNAGG 2 cut(s) 2095, 2308
BstF5I GGATG 7 cut(s) 470, 1087, 1415, 1494, 2712, 3131, 3358
BstFNI CGCG 3 cut(s) 133, 411, 2608
BstH2I RGCGCY 1 cut(s) 234
BstHHI GCGC 2 cut(s) 135, 233
BstMAI GTCTC 5 cut(s) 630, 804, 839, 1889, 2814
BstMWI GCNNNNNNNGC 2 cut(s) 1498, 3041
BstNI CCWGG 6 cut(s) 1005, 1548, 2744, 2791, 2856, 3003
BstNSI RCATGY 5 cut(s) 1362, 1501, 2207, 2420, 2953
BstPAI GACNNNNGTC 1 cut(s) 1567
BstPI GGTNACC 1 cut(s) 3234
BstSCI CCNGG 8 cut(s) 1003, 1546, 2742, 2789, 2854, 3001, 3417, 3418
BstSFI CTRYAG 1 cut(s) 2237
BstSLI GKGCMC 2 cut(s) 1602, 1891
BstUI CGCG 3 cut(s) 133, 411, 2608
BstV1I GCAGC 3 cut(s) 2621, 2624, 3311
BstV2I GAAGAC 2 cut(s) 476, 3153
BstX2I RGATCY 5 cut(s) 385, 1923, 2104, 2317, 3436
BstXI CCANNNNNNTGG 1 cut(s) 3256
BstYI RGATCY 5 cut(s) 385, 1923, 2104, 2317, 3436
BstZ17I GTATAC 2 cut(s) 364, 3072
BsuI GTATCC 1 cut(s) 121
BsuRI GGCC 4 cut(s) 20, 150, 204, 3360
BtgZI GCGATG 1 cut(s) 1995
BtsCI GGATG 7 cut(s) 470, 1087, 1415, 1494, 2712, 3131, 3358
BtsI GCAGTG 1 cut(s) 2053
BtsIMutI CAGTG 5 cut(s) 341, 1605, 1750, 2053, 2939
BveI ACCTGC 2 cut(s) 822, 2951
Cac8I GCNNGC 2 cut(s) 1499, 2632
CciI TCATGA 1 cut(s) 544
CfoI GCGC 2 cut(s) 135, 233
Cfr13I GGNCC 3 cut(s) 1001, 3331, 3359
Cfr9I CCCGGG 1 cut(s) 3418
CseI GACGC 1 cut(s) 3137
CspCI CAANNNNNGTGG 1 cut(s) 30
DraI TTTAAA 3 cut(s) 241, 579, 594
DriI GACNNNNNGTC 1 cut(s) 3154
Eam1104I CTCTTC 7 cut(s) 28, 34, 64, 246, 1112, 2073, 2819
Eam1105I GACNNNNNGTC 1 cut(s) 3154
EarI CTCTTC 7 cut(s) 28, 34, 64, 246, 1112, 2073, 2819
Ecl136II GAGCTC 1 cut(s) 1105
Eco130I CCWWGG 1 cut(s) 726
Eco147I AGGCCT 1 cut(s) 20
Eco24I GRGCYC 1 cut(s) 1107
Eco31I GGTCTC 1 cut(s) 804
Eco32I GATATC 1 cut(s) 2153
Eco47I GGWCC 2 cut(s) 1001, 3331
Eco47III AGCGCT 1 cut(s) 232
Eco53kI GAGCTC 1 cut(s) 1105
Eco57I CTGAAG 3 cut(s) 1113, 1206, 3453
Eco88I CYCGRG 3 cut(s) 2254, 2467, 3418
Eco91I GGTNACC 1 cut(s) 3234
EcoICRI GAGCTC 1 cut(s) 1105
EcoNI CCTNNNNNAGG 2 cut(s) 2095, 2308
EcoO65I GGTNACC 1 cut(s) 3234
EcoRII CCWGG 6 cut(s) 1003, 1546, 2742, 2789, 2854, 3001
EcoRV GATATC 1 cut(s) 2153
EcoT14I CCWWGG 1 cut(s) 726
EcoT22I ATGCAT 3 cut(s) 1123, 1261, 1494
EcoT38I GRGCYC 1 cut(s) 1107
ErhI CCWWGG 1 cut(s) 726
FaqI GGGAC 4 cut(s) 614, 1438, 3418, 3437
FauI CCCGC 1 cut(s) 3320
FauNDI CATATG 1 cut(s) 1140
FblI GTMKAC 2 cut(s) 363, 3071
Fnu4HI GCNGC 5 cut(s) 2635, 2638, 2947, 3242, 3325
FokI GGATG 7 cut(s) 477, 1074, 1422, 1501, 2719, 3118, 3365
FriOI GRGCYC 1 cut(s) 1107
Fsp4HI GCNGC 5 cut(s) 2635, 2638, 2947, 3242, 3325
FspBI CTAG 5 cut(s) 851, 1199, 2010, 2450, 2901
GlaI GCGC 2 cut(s) 134, 232
GluI GCNGC 5 cut(s) 2635, 2638, 2947, 3242, 3325
GsaI CCCAGC 1 cut(s) 3224
HaeII RGCGCY 1 cut(s) 234
HaeIII GGCC 4 cut(s) 20, 150, 204, 3360
HapII CCGG 4 cut(s) 1652, 2780, 3143, 3419
HgaI GACGC 1 cut(s) 3137
HhaI GCGC 2 cut(s) 135, 233
Hin6I GCGC 2 cut(s) 133, 231
HinP1I GCGC 2 cut(s) 133, 231
HincII GTYRAC 2 cut(s) 961, 2796
HindII GTYRAC 2 cut(s) 961, 2796
HindIII AAGCTT 1 cut(s) 1690
HpaI GTTAAC 1 cut(s) 2796
HpaII CCGG 4 cut(s) 1652, 2780, 3143, 3419
HpyCH4III ACNGT 6 cut(s) 1566, 1709, 2733, 2934, 3152, 3448
HpyCH4IV ACGT 5 cut(s) 1152, 1555, 1590, 3336, 3382
HpyF10VI GCNNNNNNNGC 2 cut(s) 1498, 3041
HpySE526I ACGT 5 cut(s) 1152, 1555, 1590, 3336, 3382
HspAI GCGC 2 cut(s) 133, 231
KspAI GTTAAC 1 cut(s) 2796
LguI GCTCTTC 1 cut(s) 1112
LmnI GCTCC 1 cut(s) 2868
Lsp1109I GCAGC 3 cut(s) 2621, 2624, 3311
LweI GCATC 6 cut(s) 1246, 1479, 1972, 3129, 3140, 3418
MaeI CTAG 5 cut(s) 851, 1199, 2010, 2450, 2901
MaeII ACGT 5 cut(s) 1152, 1555, 1590, 3336, 3382
MaeIII GTNAC 9 cut(s) 1412, 1875, 1987, 2226, 2272, 2439, 2485, 2960, 3234
MfeI CAATTG 1 cut(s) 246
MflI RGATCY 5 cut(s) 385, 1923, 2104, 2317, 3436
MhlI GDGCHC 3 cut(s) 1107, 1602, 1891
MlyI GAGTC 4 cut(s) 263, 841, 1544, 2695
MmeI TCCRAC 5 cut(s) 595, 640, 2025, 2519, 2885
Mph1103I ATGCAT 3 cut(s) 1123, 1261, 1494
MroXI GAANNNNTTC 4 cut(s) 496, 552, 1191, 1344
MslI CAYNNNNRTG 2 cut(s) 1143, 1871
MspI CCGG 4 cut(s) 1652, 2780, 3143, 3419
MspR9I CCNGG 8 cut(s) 1005, 1548, 2744, 2791, 2856, 3003, 3419, 3420
MunI CAATTG 1 cut(s) 246
Mva1269I GAATGC 1 cut(s) 1121
MvaI CCWGG 6 cut(s) 1005, 1548, 2744, 2791, 2856, 3003
MvnI CGCG 3 cut(s) 133, 411, 2608
MwoI GCNNNNNNNGC 2 cut(s) 1498, 3041
NciI CCSGG 2 cut(s) 3419, 3420
NdeI CATATG 1 cut(s) 1140
NlaIV GGNNCC 1 cut(s) 3332
NmuCI GTSAC 2 cut(s) 1412, 1875
NsiI ATGCAT 3 cut(s) 1123, 1261, 1494
NspI RCATGY 5 cut(s) 1362, 1501, 2207, 2420, 2953
NspV TTCGAA 1 cut(s) 3267
PaeI GCATGC 1 cut(s) 1501
PagI TCATGA 1 cut(s) 544
PaqCI CACCTGC 1 cut(s) 2951
PceI AGGCCT 1 cut(s) 20
PciI ACATGT 2 cut(s) 2203, 2416
PciSI GCTCTTC 1 cut(s) 1112
PctI GAATGC 1 cut(s) 1121
PdmI GAANNNNTTC 4 cut(s) 496, 552, 1191, 1344
Pfl23II CGTACG 1 cut(s) 1572
PfoI TCCNGGA 2 cut(s) 2854, 3001
PkrI GCNGC 5 cut(s) 2636, 2639, 2948, 3243, 3326
PleI GAGTC 4 cut(s) 262, 841, 1543, 2695
PpsI GAGTC 4 cut(s) 262, 841, 1543, 2695
Ppu21I YACGTR 1 cut(s) 3337
PscI ACATGT 2 cut(s) 2203, 2416
PshAI GACNNNNGTC 1 cut(s) 1567
PshBI ATTAAT 3 cut(s) 2160, 2373, 3108
PsiI TTATAA 1 cut(s) 1724
Psp124BI GAGCTC 1 cut(s) 1107
Psp6I CCWGG 6 cut(s) 1003, 1546, 2742, 2789, 2854, 3001
PspEI GGTNACC 1 cut(s) 3234
PspFI CCCAGC 1 cut(s) 3220
PspGI CCWGG 6 cut(s) 1003, 1546, 2742, 2789, 2854, 3001
PspLI CGTACG 1 cut(s) 1572
PspN4I GGNNCC 1 cut(s) 3332
PspPI GGNCC 3 cut(s) 1001, 3331, 3359
PsuI RGATCY 5 cut(s) 385, 1923, 2104, 2317, 3436
RseI CAYNNNNRTG 2 cut(s) 1143, 1871
SacI GAGCTC 1 cut(s) 1107
SapI GCTCTTC 1 cut(s) 1112
SatI GCNGC 5 cut(s) 2635, 2638, 2947, 3242, 3325
Sau96I GGNCC 3 cut(s) 1001, 3331, 3359
ScaI AGTACT 1 cut(s) 3093
SchI GAGTC 4 cut(s) 263, 841, 1544, 2695
ScrFI CCNGG 8 cut(s) 1005, 1548, 2744, 2791, 2856, 3003, 3419, 3420
SduI GDGCHC 3 cut(s) 1107, 1602, 1891
SfaNI GCATC 6 cut(s) 1246, 1479, 1972, 3129, 3140, 3418
SfcI CTRYAG 1 cut(s) 2237
SfuI TTCGAA 1 cut(s) 3267
SinI GGWCC 2 cut(s) 1001, 3331
SmaI CCCGGG 1 cut(s) 3420
SmiI ATTTAAAT 2 cut(s) 579, 594
SmiMI CAYNNNNRTG 2 cut(s) 1143, 1871
SmlI CTYRAG 3 cut(s) 1100, 1853, 1892
SmoI CTYRAG 3 cut(s) 1100, 1853, 1892
SphI GCATGC 1 cut(s) 1501
SseBI AGGCCT 1 cut(s) 20
SspI AATATT 1 cut(s) 1814
SspMI CTAG 5 cut(s) 851, 1199, 2010, 2450, 2901
SstI GAGCTC 1 cut(s) 1107
StuI AGGCCT 1 cut(s) 20
StyD4I CCNGG 8 cut(s) 1003, 1546, 2742, 2789, 2854, 3001, 3417, 3418
StyI CCWWGG 1 cut(s) 726
SwaI ATTTAAAT 2 cut(s) 579, 594
TaaI ACNGT 6 cut(s) 1566, 1709, 2733, 2934, 3152, 3448
TaiI ACGT 5 cut(s) 1155, 1558, 1593, 3339, 3385
TaqI TCGA 8 cut(s) 743, 1291, 1331, 1965, 2522, 3201, 3267, 3288
TatI WGTACW 5 cut(s) 156, 673, 1849, 3091, 3186
TauI GCSGC 2 cut(s) 2949, 3244
TscAI CASTG 5 cut(s) 341, 1612, 1750, 2053, 2939
TseFI GTSAC 2 cut(s) 1412, 1875
TseI GCWGC 3 cut(s) 2634, 2637, 3324
Tsp45I GTSAC 2 cut(s) 1412, 1875
TspMI CCCGGG 1 cut(s) 3418
TspRI CASTG 5 cut(s) 341, 1612, 1750, 2053, 2939
VneI GTGCAC 1 cut(s) 1598
VpaK11BI GGWCC 2 cut(s) 1001, 3331
VspI ATTAAT 3 cut(s) 2160, 2373, 3108
XagI CCTNNNNNAGG 2 cut(s) 2095, 2308
XbaI TCTAGA 2 cut(s) 2009, 2449
XceI RCATGY 5 cut(s) 1362, 1501, 2207, 2420, 2953
XmaI CCCGGG 1 cut(s) 3418
XmiI GTMKAC 2 cut(s) 363, 3071
XmnI GAANNNNTTC 4 cut(s) 496, 552, 1191, 1344
XspI CTAG 5 cut(s) 851, 1199, 2010, 2450, 2901
ZrmI AGTACT 1 cut(s) 3093
Zsp2I ATGCAT 3 cut(s) 1123, 1261, 1494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.