Rroxscaffold_7G00205530

Carboxylesterase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0006911
Physical Location & Seq
Reverse (-)
55097620 .. 55098519
900 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00205530.1

Sequence Viewer

Length: 666 bp
ATGGAGTCCACTAACAACGAAGTTGCTCGCGAGTTTCGCCTCTTCAAGGTATACATAGATGGTCGCGTCGAAAAGTTCCACAACACCCAAAACGTCCCACCTTCCACCGACTCCGTCACCGGCGTCCAATCCAAGGACGACGACTCGTGGGCTGCCCTAAAATGGGTCCAGTCCCACTCGGCCCGATCAGGACCTGACCCGTGGCTGAACCAGTACGCCGACTACGACCGGGTTTTCTTAGGCGGGGACAGCGGCGGAGCCAACATTTGCCACAGCCTGGCAGTTCGGGTCGGGTCAATTGGGTTTCCCGGAGTGAAAGTCGCGGGAATGGTTATGGTGCATCCCTTTTTTGGTGGGTCGGCCGTGGACGACGAAATGTGGCTGTACATGTGTCCGGAGAACGTCGAGGTGCAGGACAGGAGGCTGAGGCCGCCGGCAGAGGATCTGGCGAAGCTTGGATGCGAGAGGGTGTTGATATTTTTTGCGGAGAAGGATCATCTGAGAGAGGTAGGGCAGTGGTACTGTGATGAGCTGAAGAAGAGTGAGTGGGGTGAGAGTGTTGAAGTGGTGGAGCATGAGGATGTGTTTCATTTGAGCAGAGAACCTGAGGGAGAGAAAGCTCAGGACTTGATCAAGAAGTTTGGTTCCTTCATCAAGCAAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

221

Amino Acids

25.03

Weight (kDa)

5.04

Isoelectric Point (pI)

40.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_3 PF07859 47 - 197 1.2e-18 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 277
AccI GTMKAC 1 cut(s) 51
AccII CGCG 3 cut(s) 30, 66, 323
AccIII TCCGGA 1 cut(s) 394
AciI CCGC 6 cut(s) 243, 252, 255, 323, 431, 485
AclWI GGATC 2 cut(s) 450, 501
AcoI YGGCCR 1 cut(s) 360
AcuI CTGAAG 1 cut(s) 554
AcyI GRCGYC 1 cut(s) 123
AfaI GTAC 3 cut(s) 215, 386, 521
AfiI CCNNNNNNNGG 6 cut(s) 46, 133, 162, 163, 277, 350
AflIII ACRYGT 1 cut(s) 387
AgsI TTSAA 2 cut(s) 46, 563
AjnI CCWGG 1 cut(s) 276
AluBI AGCT 3 cut(s) 454, 532, 620
AluI AGCT 3 cut(s) 454, 532, 620
AlwI GGATC 2 cut(s) 450, 501
AlwNI CAGNNNCTG 1 cut(s) 194
Aor13HI TCCGGA 1 cut(s) 394
AoxI GGCC 3 cut(s) 180, 360, 428
ApeKI GCWGC 1 cut(s) 152
AspS9I GGNCC 3 cut(s) 166, 181, 191
AsuC2I CCSGG 2 cut(s) 230, 309
AsuHPI GGTGA 2 cut(s) 109, 563
AvaII GGWCC 2 cut(s) 166, 191
AxyI CCTNAGG 1 cut(s) 606
BauI CACGAG 1 cut(s) 145
BbvCI CCTCAGC 1 cut(s) 425
BbvI GCAGC 1 cut(s) 139
BccI CCATC 1 cut(s) 53
BceAI ACGGC 1 cut(s) 347
BciT130I CCWGG 1 cut(s) 278
BclI TGATCA 1 cut(s) 630
BcnI CCSGG 2 cut(s) 230, 309
BisI GCNGC 3 cut(s) 153, 253, 431
BlsI GCNGC 3 cut(s) 154, 254, 432
Bme1390I CCNGG 3 cut(s) 230, 278, 309
Bme18I GGWCC 2 cut(s) 166, 191
BmgT120I GGNCC 3 cut(s) 166, 181, 191
BmiI GGNNCC 3 cut(s) 167, 259, 646
BmrFI CCNGG 3 cut(s) 230, 278, 309
BmsI GCATC 2 cut(s) 349, 449
Bpu10I CCTNAGC 2 cut(s) 425, 621
BpuMI CCSGG 2 cut(s) 230, 309
BsaHI GRCGYC 1 cut(s) 123
BsaJI CCNNGG 3 cut(s) 132, 200, 363
BsaWI WCCGGW 1 cut(s) 394
BsaXI ACNNNNNCTCC 2 cut(s) 303, 333
Bsc4I CCNNNNNNNGG 6 cut(s) 46, 133, 162, 163, 277, 350
Bse118I RCCGGY 2 cut(s) 119, 433
Bse1I ACTGG 2 cut(s) 169, 211
Bse21I CCTNAGG 1 cut(s) 606
BseAI TCCGGA 1 cut(s) 394
BseBI CCWGG 1 cut(s) 278
BseDI CCNNGG 3 cut(s) 132, 200, 363
BseGI GGATG 3 cut(s) 340, 464, 586
BseLI CCNNNNNNNGG 6 cut(s) 46, 133, 162, 163, 277, 350
BseMII CTCAG 4 cut(s) 416, 491, 597, 635
BseNI ACTGG 2 cut(s) 169, 211
BseX3I CGGCCG 1 cut(s) 360
BseXI GCAGC 1 cut(s) 139
BsgI GTGCAG 1 cut(s) 431
Bsh1236I CGCG 3 cut(s) 30, 66, 323
Bsh1285I CGRYCG 2 cut(s) 229, 363
BshFI GGCC 3 cut(s) 182, 362, 430
BsiEI CGRYCG 2 cut(s) 229, 363
BsiSI CCGG 5 cut(s) 120, 229, 309, 395, 434
BslFI GGGAC 3 cut(s) 80, 157, 260
BslI CCNNNNNNNGG 6 cut(s) 46, 133, 162, 163, 277, 350
BsmFI GGGAC 3 cut(s) 80, 157, 260
BsnI GGCC 3 cut(s) 182, 362, 430
Bsp13I TCCGGA 1 cut(s) 394
Bsp1407I TGTACA 1 cut(s) 384
Bsp143I GATC 4 cut(s) 185, 442, 493, 630
Bsp68I TCGCGA 1 cut(s) 30
BspACI CCGC 6 cut(s) 243, 252, 255, 323, 431, 485
BspANI GGCC 3 cut(s) 182, 362, 430
BspCNI CTCAG 4 cut(s) 417, 492, 598, 634
BspEI TCCGGA 1 cut(s) 394
BspFNI CGCG 3 cut(s) 30, 66, 323
BspLI GGNNCC 3 cut(s) 167, 259, 646
BspPI GGATC 2 cut(s) 450, 501
BsrFI RCCGGY 2 cut(s) 119, 433
BsrGI TGTACA 1 cut(s) 384
BsrI ACTGG 2 cut(s) 169, 211
BssAI RCCGGY 2 cut(s) 119, 433
BssECI CCNNGG 3 cut(s) 132, 200, 363
BssMI GATC 4 cut(s) 185, 442, 493, 630
BssNAI GTATAC 1 cut(s) 52
BssNI GRCGYC 1 cut(s) 123
BssSI CACGAG 1 cut(s) 145
BssT1I CCWWGG 1 cut(s) 132
Bst1107I GTATAC 1 cut(s) 52
Bst2BI CACGAG 1 cut(s) 145
Bst2UI CCWGG 1 cut(s) 278
Bst4CI ACNGT 1 cut(s) 524
Bst6I CTCTTC 2 cut(s) 47, 533
BstACI GRCGYC 1 cut(s) 123
BstAUI TGTACA 1 cut(s) 384
BstC8I GCNNGC 2 cut(s) 28, 435
BstDEI CTNAG 5 cut(s) 238, 425, 500, 606, 621
BstDSI CCRYGG 2 cut(s) 200, 363
BstENI CCTNNNNNAGG 1 cut(s) 44
BstF5I GGATG 3 cut(s) 340, 464, 586
BstFNI CGCG 3 cut(s) 30, 66, 323
BstKTI GATC 4 cut(s) 188, 445, 496, 633
BstMBI GATC 4 cut(s) 185, 442, 493, 630
BstMCI CGRYCG 2 cut(s) 229, 363
BstMWI GCNNNNNNNGC 3 cut(s) 36, 249, 430
BstNI CCWGG 1 cut(s) 278
BstNSI RCATGY 1 cut(s) 391
BstSCI CCNGG 3 cut(s) 228, 276, 307
BstUI CGCG 3 cut(s) 30, 66, 323
BstV1I GCAGC 1 cut(s) 139
BstX2I RGATCY 1 cut(s) 442
BstYI RGATCY 1 cut(s) 442
BstZ17I GTATAC 1 cut(s) 52
BstZI CGGCCG 1 cut(s) 360
Bsu36I CCTNAGG 1 cut(s) 606
BsuRI GGCC 3 cut(s) 182, 362, 430
BtgI CCRYGG 2 cut(s) 200, 363
BtsCI GGATG 3 cut(s) 340, 464, 586
BtsI GCAGTG 1 cut(s) 521
BtsIMutI CAGTG 1 cut(s) 521
BtuMI TCGCGA 1 cut(s) 30
Cac8I GCNNGC 2 cut(s) 28, 435
CaiI CAGNNNCTG 1 cut(s) 194
Cfr10I RCCGGY 2 cut(s) 119, 433
Cfr13I GGNCC 3 cut(s) 166, 181, 191
CseI GACGC 2 cut(s) 55, 112
Csp6I GTAC 3 cut(s) 214, 385, 520
CviAII CATG 2 cut(s) 388, 575
CviQI GTAC 3 cut(s) 214, 385, 520
DdeI CTNAG 5 cut(s) 238, 425, 500, 606, 621
DpnI GATC 4 cut(s) 187, 444, 495, 632
DpnII GATC 4 cut(s) 185, 442, 493, 630
EaeI YGGCCR 1 cut(s) 360
EagI CGGCCG 1 cut(s) 360
Eam1104I CTCTTC 2 cut(s) 47, 533
EarI CTCTTC 2 cut(s) 47, 533
EciI GGCGGA 1 cut(s) 270
EclXI CGGCCG 1 cut(s) 360
Eco130I CCWWGG 1 cut(s) 132
Eco47I GGWCC 2 cut(s) 166, 191
Eco52I CGGCCG 1 cut(s) 360
Eco57I CTGAAG 1 cut(s) 554
Eco81I CCTNAGG 1 cut(s) 606
EcoNI CCTNNNNNAGG 1 cut(s) 44
EcoO109I RGGNCCY 1 cut(s) 191
EcoRII CCWGG 1 cut(s) 276
EcoT14I CCWWGG 1 cut(s) 132
ErhI CCWWGG 1 cut(s) 132
FaeI CATG 2 cut(s) 391, 578
FaiI YATR 5 cut(s) 52, 56, 335, 389, 576
FaqI GGGAC 3 cut(s) 80, 157, 260
FatI CATG 2 cut(s) 387, 574
FauI CCCGC 2 cut(s) 236, 316
FbaI TGATCA 1 cut(s) 630
FblI GTMKAC 1 cut(s) 51
Fnu4HI GCNGC 3 cut(s) 153, 253, 431
FokI GGATG 3 cut(s) 327, 471, 593
Fsp4HI GCNGC 3 cut(s) 153, 253, 431
GluI GCNGC 3 cut(s) 153, 253, 431
HaeIII GGCC 3 cut(s) 182, 362, 430
HapII CCGG 5 cut(s) 120, 229, 309, 395, 434
HgaI GACGC 2 cut(s) 55, 112
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 2 cut(s) 391, 578
HindIII AAGCTT 1 cut(s) 452
HinfI GANTC 3 cut(s) 5, 110, 143
HpaII CCGG 5 cut(s) 120, 229, 309, 395, 434
HphI GGTGA 2 cut(s) 109, 563
Hpy166II GTNNAC 3 cut(s) 9, 52, 367
Hpy188I TCNGA 1 cut(s) 501
Hpy188III TCNNGA 5 cut(s) 29, 189, 395, 623, 634
Hpy8I GTNNAC 3 cut(s) 9, 52, 367
Hpy99I CGWCG 4 cut(s) 71, 143, 374, 407
HpyAV CCTTC 3 cut(s) 111, 484, 658
HpyCH4III ACNGT 1 cut(s) 524
HpyCH4IV ACGT 2 cut(s) 93, 402
HpyCH4V TGCA 2 cut(s) 340, 412
HpyF10VI GCNNNNNNNGC 3 cut(s) 36, 249, 430
HpyF3I CTNAG 5 cut(s) 238, 425, 500, 606, 621
HpySE526I ACGT 2 cut(s) 93, 402
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 2 cut(s) 391, 578
Kpn2I TCCGGA 1 cut(s) 394
KroI GCCGGC 1 cut(s) 433
KroNI GCCGGC 1 cut(s) 435
Ksp22I TGATCA 1 cut(s) 630
Kzo9I GATC 4 cut(s) 185, 442, 493, 630
LmnI GCTCC 2 cut(s) 257, 571
Lsp1109I GCAGC 1 cut(s) 139
LweI GCATC 2 cut(s) 349, 449
MaeII ACGT 2 cut(s) 93, 402
MaeIII GTNAC 1 cut(s) 115
MalI GATC 4 cut(s) 187, 444, 495, 632
MboI GATC 4 cut(s) 185, 442, 493, 630
MboII GAAGA 3 cut(s) 34, 547, 550
MfeI CAATTG 1 cut(s) 297
MflI RGATCY 1 cut(s) 442
MluCI AATT 1 cut(s) 297
MlyI GAGTC 3 cut(s) 14, 104, 137
MnlI CCTC 9 cut(s) 50, 400, 414, 420, 433, 459, 499, 571, 601
MroI TCCGGA 1 cut(s) 394
MroNI GCCGGC 1 cut(s) 433
MslI CAYNNNNRTG 1 cut(s) 579
MspA1I CMGCKG 1 cut(s) 252
MspI CCGG 5 cut(s) 120, 229, 309, 395, 434
MspR9I CCNGG 3 cut(s) 230, 278, 309
MunI CAATTG 1 cut(s) 297
MvaI CCWGG 1 cut(s) 278
MvnI CGCG 3 cut(s) 30, 66, 323
MwoI GCNNNNNNNGC 3 cut(s) 36, 249, 430
NaeI GCCGGC 1 cut(s) 435
NciI CCSGG 2 cut(s) 230, 309
NdeII GATC 4 cut(s) 185, 442, 493, 630
NgoMIV GCCGGC 1 cut(s) 433
NlaIII CATG 2 cut(s) 391, 578
NlaIV GGNNCC 3 cut(s) 167, 259, 646
NmeAIII GCCGAG 1 cut(s) 158
NmuCI GTSAC 1 cut(s) 115
NruI TCGCGA 1 cut(s) 30
NspI RCATGY 1 cut(s) 391
PciI ACATGT 1 cut(s) 387
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PdiI GCCGGC 1 cut(s) 435
PflFI GACNNNGTC 1 cut(s) 113
PflMI CCANNNNNTGG 1 cut(s) 277
PfoI TCCNGGA 1 cut(s) 307
PkrI GCNGC 3 cut(s) 154, 254, 432
PleI GAGTC 3 cut(s) 13, 104, 137
PpsI GAGTC 3 cut(s) 13, 104, 137
PpuMI RGGWCCY 1 cut(s) 191
PscI ACATGT 1 cut(s) 387
Psp5II RGGWCCY 1 cut(s) 191
Psp6I CCWGG 1 cut(s) 276
PspGI CCWGG 1 cut(s) 276
PspN4I GGNNCC 3 cut(s) 167, 259, 646
PspPI GGNCC 3 cut(s) 166, 181, 191
PspPPI RGGWCCY 1 cut(s) 191
PstNI CAGNNNCTG 1 cut(s) 194
PsuI RGATCY 1 cut(s) 442
PsyI GACNNNGTC 1 cut(s) 113
RruI TCGCGA 1 cut(s) 30
RsaI GTAC 3 cut(s) 215, 386, 521
RsaNI GTAC 3 cut(s) 214, 385, 520
RseI CAYNNNNRTG 1 cut(s) 579
SatI GCNGC 3 cut(s) 153, 253, 431
Sau3AI GATC 4 cut(s) 185, 442, 493, 630
Sau96I GGNCC 3 cut(s) 166, 181, 191
SchI GAGTC 3 cut(s) 14, 104, 137
ScrFI CCNGG 3 cut(s) 230, 278, 309
SfaNI GCATC 2 cut(s) 349, 449
SgrAI CRCCGGYG 1 cut(s) 119
SinI GGWCC 2 cut(s) 166, 191
SmiMI CAYNNNNRTG 1 cut(s) 579
Sse9I AATT 1 cut(s) 297
SsiI CCGC 6 cut(s) 243, 252, 255, 323, 431, 485
StyD4I CCNGG 3 cut(s) 228, 276, 307
StyI CCWWGG 1 cut(s) 132
TaaI ACNGT 1 cut(s) 524
TaiI ACGT 2 cut(s) 96, 405
TaqI TCGA 2 cut(s) 69, 405
TasI AATT 1 cut(s) 297
TatI WGTACW 1 cut(s) 384
TauI GCSGC 2 cut(s) 255, 433
TscAI CASTG 1 cut(s) 521
TseFI GTSAC 1 cut(s) 115
TseI GCWGC 1 cut(s) 152
Tsp45I GTSAC 1 cut(s) 115
TspDTI ATGAA 2 cut(s) 578, 640
TspGWI ACGGA 1 cut(s) 103
TspRI CASTG 1 cut(s) 521
Tth111I GACNNNGTC 1 cut(s) 113
Van91I CCANNNNNTGG 1 cut(s) 277
VpaK11BI GGWCC 2 cut(s) 166, 191
XagI CCTNNNNNAGG 1 cut(s) 44
XceI RCATGY 1 cut(s) 391
XmiI GTMKAC 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.