Rw6G008450

Carboxylesterase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0006911
Physical Location & Seq
Reverse (-)
14332157 .. 14333465
1309 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G008450.1

Sequence Viewer

Length: 639 bp
ATGTTTCACAAGCATCTCACCTCCTTAGCCGCCGAAGCCAACGCCATTGCCTTCTCCGTCGAATACGGGCTGTTCCCGGATCGACCCATACCCGCTTGCTACGACGACTCGTGGGCCGCCCTCAAATGGGTCCAGTCCCAATCGACCCGATCAGGACCTGACCCGTGGCTGAACCAGTACGCCGACTTTAACCGGTTTTTCTTAGGCCGGGACAGCGGCGGAGCCAACATTTGCCACAGCCTGGCAGTTCGGGTCGGGTCAATAGGGTTACCCGGAGTGAAAGTCGCGGGAATGGTTATGGTGCATCCGTTTTTTGGTGGGTCGGCCGTGGACGACGAAATGTGGCTGTACATGTGTCCGGAGAACGGCGGGGTGCAGGACAGGAGGCTGAGGCCGCCGGCAGAGGATCTGGCCGAGCTTGGATGCGAGAGGGTGTTGATATTTTTTGCGGAGAAGGATCATCTGAGAGAGGTAGGTCAGTGGTACTGCGATGAGCTGAAGAAGAGTGAGTGGGGTGGGAGTGTTGAAGTGGTGGAGCATGAGGATGAGTTTCATGTGTTTCATTTGCGCAGAGAACCTGAGGGAGAGAAAGCTCAGGACTTGATCAAGAAGTTTGGTTCCTTCATCAAGCAAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

212

Amino Acids

23.95

Weight (kDa)

5.25

Isoelectric Point (pI)

47.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_3 PF07859 3 - 188 1e-28 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 569
AccB7I CCANNNNNTGG 1 cut(s) 241
AccII CGCG 1 cut(s) 287
AccIII TCCGGA 1 cut(s) 358
AciI CCGC 9 cut(s) 30, 93, 117, 216, 219, 287, 369, 395, 449
AclWI GGATC 3 cut(s) 87, 414, 465
AcoI YGGCCR 2 cut(s) 324, 411
AcuI CTGAAG 1 cut(s) 518
AfaI GTAC 3 cut(s) 179, 350, 485
AfiI CCNNNNNNNGG 5 cut(s) 126, 127, 241, 314, 365
AflIII ACRYGT 1 cut(s) 351
AgeI ACCGGT 1 cut(s) 192
AgsI TTSAA 1 cut(s) 527
AjnI CCWGG 1 cut(s) 240
AluBI AGCT 3 cut(s) 418, 496, 593
AluI AGCT 3 cut(s) 418, 496, 593
AlwI GGATC 3 cut(s) 87, 414, 465
AlwNI CAGNNNCTG 1 cut(s) 158
Aor13HI TCCGGA 1 cut(s) 358
AoxI GGCC 5 cut(s) 114, 205, 324, 392, 411
AsiGI ACCGGT 1 cut(s) 192
AspLEI GCGC 1 cut(s) 570
AspS9I GGNCC 3 cut(s) 114, 130, 155
AsuC2I CCSGG 3 cut(s) 77, 209, 273
AsuHPI GGTGA 1 cut(s) 10
AvaII GGWCC 2 cut(s) 130, 155
AxyI CCTNAGG 1 cut(s) 579
BauI CACGAG 1 cut(s) 109
BbvCI CCTCAGC 1 cut(s) 389
BceAI ACGGC 2 cut(s) 311, 382
BciT130I CCWGG 1 cut(s) 242
BclI TGATCA 1 cut(s) 603
BcnI CCSGG 3 cut(s) 77, 209, 273
BisI GCNGC 4 cut(s) 30, 117, 217, 395
BlsI GCNGC 4 cut(s) 31, 118, 218, 396
Bme1390I CCNGG 4 cut(s) 77, 209, 242, 273
Bme18I GGWCC 2 cut(s) 130, 155
BmgT120I GGNCC 3 cut(s) 114, 130, 155
BmiI GGNNCC 3 cut(s) 131, 223, 619
BmrFI CCNGG 4 cut(s) 77, 209, 242, 273
BmsI GCATC 3 cut(s) 22, 313, 413
Bpu10I CCTNAGC 3 cut(s) 25, 389, 594
BpuMI CCSGG 3 cut(s) 77, 209, 273
BsaJI CCNNGG 2 cut(s) 164, 327
BsaWI WCCGGW 2 cut(s) 192, 358
BsaXI ACNNNNNCTCC 2 cut(s) 267, 297
Bsc4I CCNNNNNNNGG 5 cut(s) 126, 127, 241, 314, 365
Bse118I RCCGGY 2 cut(s) 192, 397
Bse1I ACTGG 2 cut(s) 133, 175
Bse21I CCTNAGG 1 cut(s) 579
Bse3DI GCAATG 1 cut(s) 45
BseAI TCCGGA 1 cut(s) 358
BseBI CCWGG 1 cut(s) 242
BseDI CCNNGG 2 cut(s) 164, 327
BseGI GGATG 3 cut(s) 304, 428, 550
BseLI CCNNNNNNNGG 5 cut(s) 126, 127, 241, 314, 365
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 4 cut(s) 380, 455, 570, 608
BseNI ACTGG 2 cut(s) 133, 175
BseX3I CGGCCG 1 cut(s) 324
BsgI GTGCAG 1 cut(s) 395
Bsh1236I CGCG 1 cut(s) 287
Bsh1285I CGRYCG 1 cut(s) 327
BshFI GGCC 5 cut(s) 116, 207, 326, 394, 413
BshTI ACCGGT 1 cut(s) 192
BsiEI CGRYCG 1 cut(s) 327
BsiSI CCGG 6 cut(s) 77, 193, 208, 273, 359, 398
BslFI GGGAC 2 cut(s) 121, 224
BslI CCNNNNNNNGG 5 cut(s) 126, 127, 241, 314, 365
BsmFI GGGAC 2 cut(s) 121, 224
BsnI GGCC 5 cut(s) 116, 207, 326, 394, 413
Bsp13I TCCGGA 1 cut(s) 358
Bsp1407I TGTACA 1 cut(s) 348
Bsp143I GATC 5 cut(s) 79, 149, 406, 457, 603
BspACI CCGC 9 cut(s) 30, 93, 117, 216, 219, 287, 369, 395, 449
BspANI GGCC 5 cut(s) 116, 207, 326, 394, 413
BspCNI CTCAG 4 cut(s) 381, 456, 571, 607
BspEI TCCGGA 1 cut(s) 358
BspFNI CGCG 1 cut(s) 287
BspLI GGNNCC 3 cut(s) 131, 223, 619
BspPI GGATC 3 cut(s) 87, 414, 465
BsrDI GCAATG 1 cut(s) 45
BsrFI RCCGGY 2 cut(s) 192, 397
BsrGI TGTACA 1 cut(s) 348
BsrI ACTGG 2 cut(s) 133, 175
BssAI RCCGGY 2 cut(s) 192, 397
BssECI CCNNGG 2 cut(s) 164, 327
BssMI GATC 5 cut(s) 79, 149, 406, 457, 603
BssSI CACGAG 1 cut(s) 109
Bst2BI CACGAG 1 cut(s) 109
Bst2UI CCWGG 1 cut(s) 242
Bst6I CTCTTC 1 cut(s) 497
BstAUI TGTACA 1 cut(s) 348
BstC8I GCNNGC 2 cut(s) 97, 399
BstDEI CTNAG 6 cut(s) 25, 202, 389, 464, 579, 594
BstDSI CCRYGG 2 cut(s) 164, 327
BstEII GGTNACC 1 cut(s) 267
BstF5I GGATG 3 cut(s) 304, 428, 550
BstFNI CGCG 1 cut(s) 287
BstHHI GCGC 1 cut(s) 570
BstKTI GATC 5 cut(s) 82, 152, 409, 460, 606
BstMBI GATC 5 cut(s) 79, 149, 406, 457, 603
BstMCI CGRYCG 1 cut(s) 327
BstMWI GCNNNNNNNGC 3 cut(s) 35, 213, 394
BstNI CCWGG 1 cut(s) 242
BstNSI RCATGY 1 cut(s) 355
BstPI GGTNACC 1 cut(s) 267
BstSCI CCNGG 4 cut(s) 75, 207, 240, 271
BstUI CGCG 1 cut(s) 287
BstX2I RGATCY 1 cut(s) 406
BstYI RGATCY 1 cut(s) 406
BstZI CGGCCG 1 cut(s) 324
Bsu36I CCTNAGG 1 cut(s) 579
BsuRI GGCC 5 cut(s) 116, 207, 326, 394, 413
BtgI CCRYGG 2 cut(s) 164, 327
BtgZI GCGATG 1 cut(s) 504
BtsCI GGATG 3 cut(s) 304, 428, 550
BtsIMutI CAGTG 1 cut(s) 485
Cac8I GCNNGC 2 cut(s) 97, 399
CaiI CAGNNNCTG 1 cut(s) 158
CfoI GCGC 1 cut(s) 570
Cfr10I RCCGGY 2 cut(s) 192, 397
Cfr13I GGNCC 3 cut(s) 114, 130, 155
Csp6I GTAC 3 cut(s) 178, 349, 484
CspAI ACCGGT 1 cut(s) 192
CviAII CATG 3 cut(s) 352, 539, 554
CviQI GTAC 3 cut(s) 178, 349, 484
DdeI CTNAG 6 cut(s) 25, 202, 389, 464, 579, 594
DpnI GATC 5 cut(s) 81, 151, 408, 459, 605
DpnII GATC 5 cut(s) 79, 149, 406, 457, 603
EaeI YGGCCR 2 cut(s) 324, 411
EagI CGGCCG 1 cut(s) 324
Eam1104I CTCTTC 1 cut(s) 497
EarI CTCTTC 1 cut(s) 497
EciI GGCGGA 1 cut(s) 234
EclXI CGGCCG 1 cut(s) 324
Eco47I GGWCC 2 cut(s) 130, 155
Eco52I CGGCCG 1 cut(s) 324
Eco57I CTGAAG 1 cut(s) 518
Eco81I CCTNAGG 1 cut(s) 579
Eco91I GGTNACC 1 cut(s) 267
EcoO109I RGGNCCY 1 cut(s) 155
EcoO65I GGTNACC 1 cut(s) 267
EcoRII CCWGG 1 cut(s) 240
FaeI CATG 3 cut(s) 355, 542, 557
FaiI YATR 5 cut(s) 89, 299, 353, 540, 555
FaqI GGGAC 2 cut(s) 121, 224
FatI CATG 3 cut(s) 351, 538, 553
FauI CCCGC 3 cut(s) 100, 280, 362
FbaI TGATCA 1 cut(s) 603
Fnu4HI GCNGC 4 cut(s) 30, 117, 217, 395
FokI GGATG 3 cut(s) 291, 435, 557
Fsp4HI GCNGC 4 cut(s) 30, 117, 217, 395
FspI TGCGCA 1 cut(s) 569
GlaI GCGC 1 cut(s) 569
GluI GCNGC 4 cut(s) 30, 117, 217, 395
HaeIII GGCC 5 cut(s) 116, 207, 326, 394, 413
HapII CCGG 6 cut(s) 77, 193, 208, 273, 359, 398
HhaI GCGC 1 cut(s) 570
Hin1II CATG 3 cut(s) 355, 542, 557
Hin6I GCGC 1 cut(s) 568
HinP1I GCGC 1 cut(s) 568
HinfI GANTC 1 cut(s) 107
HpaII CCGG 6 cut(s) 77, 193, 208, 273, 359, 398
HphI GGTGA 1 cut(s) 10
Hpy166II GTNNAC 1 cut(s) 331
Hpy188I TCNGA 1 cut(s) 465
Hpy188III TCNNGA 4 cut(s) 153, 359, 596, 607
Hpy8I GTNNAC 1 cut(s) 331
Hpy99I CGWCG 3 cut(s) 62, 107, 338
HpyAV CCTTC 3 cut(s) 61, 448, 631
HpyCH4V TGCA 2 cut(s) 304, 376
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 213, 394
HpyF3I CTNAG 6 cut(s) 25, 202, 389, 464, 579, 594
Hsp92II CATG 3 cut(s) 355, 542, 557
HspAI GCGC 1 cut(s) 568
Kpn2I TCCGGA 1 cut(s) 358
KroI GCCGGC 1 cut(s) 397
KroNI GCCGGC 1 cut(s) 399
Ksp22I TGATCA 1 cut(s) 603
Kzo9I GATC 5 cut(s) 79, 149, 406, 457, 603
LmnI GCTCC 2 cut(s) 221, 535
LweI GCATC 3 cut(s) 22, 313, 413
MaeIII GTNAC 1 cut(s) 267
MalI GATC 5 cut(s) 81, 151, 408, 459, 605
MboI GATC 5 cut(s) 79, 149, 406, 457, 603
MboII GAAGA 2 cut(s) 511, 514
MflI RGATCY 1 cut(s) 406
MlyI GAGTC 1 cut(s) 101
MnlI CCTC 9 cut(s) 31, 131, 378, 384, 397, 423, 463, 535, 574
MroI TCCGGA 1 cut(s) 358
MroNI GCCGGC 1 cut(s) 397
MseI TTAA 1 cut(s) 189
MslI CAYNNNNRTG 1 cut(s) 543
MspA1I CMGCKG 1 cut(s) 216
MspI CCGG 6 cut(s) 77, 193, 208, 273, 359, 398
MspR9I CCNGG 4 cut(s) 77, 209, 242, 273
MvaI CCWGG 1 cut(s) 242
MvnI CGCG 1 cut(s) 287
MwoI GCNNNNNNNGC 3 cut(s) 35, 213, 394
NaeI GCCGGC 1 cut(s) 399
NciI CCSGG 3 cut(s) 77, 209, 273
NdeII GATC 5 cut(s) 79, 149, 406, 457, 603
NgoMIV GCCGGC 1 cut(s) 397
NlaIII CATG 3 cut(s) 355, 542, 557
NlaIV GGNNCC 3 cut(s) 131, 223, 619
NmeAIII GCCGAG 1 cut(s) 439
NsbI TGCGCA 1 cut(s) 569
NspI RCATGY 1 cut(s) 355
PciI ACATGT 1 cut(s) 351
PdiI GCCGGC 1 cut(s) 399
PflMI CCANNNNNTGG 1 cut(s) 241
PfoI TCCNGGA 1 cut(s) 75
PinAI ACCGGT 1 cut(s) 192
PkrI GCNGC 4 cut(s) 31, 118, 218, 396
PleI GAGTC 1 cut(s) 101
PpsI GAGTC 1 cut(s) 101
PpuMI RGGWCCY 1 cut(s) 155
PscI ACATGT 1 cut(s) 351
Psp5II RGGWCCY 1 cut(s) 155
Psp6I CCWGG 1 cut(s) 240
PspEI GGTNACC 1 cut(s) 267
PspGI CCWGG 1 cut(s) 240
PspN4I GGNNCC 3 cut(s) 131, 223, 619
PspPI GGNCC 3 cut(s) 114, 130, 155
PspPPI RGGWCCY 1 cut(s) 155
PstNI CAGNNNCTG 1 cut(s) 158
PsuI RGATCY 1 cut(s) 406
RsaI GTAC 3 cut(s) 179, 350, 485
RsaNI GTAC 3 cut(s) 178, 349, 484
RseI CAYNNNNRTG 1 cut(s) 543
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 4 cut(s) 30, 117, 217, 395
Sau3AI GATC 5 cut(s) 79, 149, 406, 457, 603
Sau96I GGNCC 3 cut(s) 114, 130, 155
SchI GAGTC 1 cut(s) 101
ScrFI CCNGG 4 cut(s) 77, 209, 242, 273
SetI ASST 8 cut(s) 23, 160, 420, 474, 478, 498, 580, 595
SfaNI GCATC 3 cut(s) 22, 313, 413
SinI GGWCC 2 cut(s) 130, 155
SmiMI CAYNNNNRTG 1 cut(s) 543
SsiI CCGC 9 cut(s) 30, 93, 117, 216, 219, 287, 369, 395, 449
StyD4I CCNGG 4 cut(s) 75, 207, 240, 271
TaqI TCGA 3 cut(s) 60, 82, 143
TatI WGTACW 1 cut(s) 348
TauI GCSGC 4 cut(s) 32, 119, 219, 397
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TscAI CASTG 1 cut(s) 485
TspDTI ATGAA 3 cut(s) 542, 551, 613
TspGWI ACGGA 2 cut(s) 46, 297
TspRI CASTG 1 cut(s) 485
Van91I CCANNNNNTGG 1 cut(s) 241
VpaK11BI GGWCC 2 cut(s) 130, 155
XceI RCATGY 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.