FvH4_2g15480

Protein of unknown function (DUF1279)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010849
Physical Location & Seq
Forward (+)
13617092 .. 13619681
2590 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g15480.t1

Sequence Viewer

Length: 399 bp
ATGGCCGGTGGTCGCTTCCGCGAGCTTTTGAAGAAGTACGGCAAGGTGGCGCTGGGGGTCCACTGCTCCGTCTCGGCCGCCTCCATCGCCGGACTCTACGTCGCCATCAAGAGCAACGTCGACGTCGAGTCGCTGATTGAGAAGCTCCCCAACCTAAACCCTCTTAAATCCTCTACCGAAGAGAAACCCACCTCGGAAAGTGGGCCCTCGGCGGCTGTGGTGGAGGAGAAGAAGAGAAACCGGACGGCGGAGCTGGCGGCCACAAGCGGCGGCGCTCTGGCATTGGCTTTTCTCTGCAACAAGGCTCTGTTTCCGATTCGGGTTCCGATTACGCTGGCGGTTACGCCTCCTTTGGCTAAGTTCTTGGCCAGGAGGAGGCTTATCAAGACTGGTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1279

Protein Analysis

133

Amino Acids

13.99

Weight (kDa)

10.01

Isoelectric Point (pI)

40.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAM210A-B_dom PF06916 5 - 116 2.7e-24 FAM210A/B-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 126
AccI GTMKAC 1 cut(s) 120
AccII CGCG 1 cut(s) 21
AciI CCGC 8 cut(s) 19, 78, 212, 248, 257, 267, 270, 338
AcoI YGGCCR 4 cut(s) 3, 75, 258, 366
AcyI GRCGYC 1 cut(s) 123
AfaI GTAC 1 cut(s) 38
AfiI CCNNNNNNNGG 2 cut(s) 247, 375
AgsI TTSAA 1 cut(s) 31
AhdI GACNNNNNGTC 2 cut(s) 98, 127
AjnI CCWGG 1 cut(s) 368
AluBI AGCT 3 cut(s) 25, 145, 253
AluI AGCT 3 cut(s) 25, 145, 253
Alw26I GTCTC 1 cut(s) 76
AoxI GGCC 5 cut(s) 3, 75, 203, 258, 366
ApaI GGGCCC 1 cut(s) 207
AspLEI GCGC 2 cut(s) 52, 275
AspS9I GGNCC 3 cut(s) 58, 203, 204
AvaII GGWCC 1 cut(s) 58
BaeGI GKGCMC 1 cut(s) 207
BalI TGGCCA 1 cut(s) 368
BanII GRGCYC 1 cut(s) 207
BccI CCATC 2 cut(s) 92, 113
BceAI ACGGC 2 cut(s) 55, 261
BciT130I CCWGG 1 cut(s) 370
BcoDI GTCTC 1 cut(s) 76
BfoI RGCGCY 2 cut(s) 53, 276
BisI GCNGC 5 cut(s) 78, 213, 258, 268, 271
BlsI GCNGC 5 cut(s) 79, 214, 259, 269, 272
Bme1390I CCNGG 1 cut(s) 370
Bme18I GGWCC 1 cut(s) 58
BmeRI GACNNNNNGTC 2 cut(s) 98, 127
BmgT120I GGNCC 3 cut(s) 58, 203, 204
BmiI GGNNCC 3 cut(s) 59, 205, 324
BmrFI CCNGG 1 cut(s) 370
BsaHI GRCGYC 1 cut(s) 123
BsaJI CCNNGG 2 cut(s) 192, 207
BsaWI WCCGGW 1 cut(s) 240
Bsc4I CCNNNNNNNGG 2 cut(s) 247, 375
Bse118I RCCGGY 1 cut(s) 5
Bse1I ACTGG 1 cut(s) 394
BseBI CCWGG 1 cut(s) 370
BseDI CCNNGG 2 cut(s) 192, 207
BseLI CCNNNNNNNGG 2 cut(s) 247, 375
BseNI ACTGG 1 cut(s) 394
BseRI GAGGAG 2 cut(s) 239, 388
BseSI GKGCMC 1 cut(s) 207
BseX3I CGGCCG 1 cut(s) 75
BseYI CCCAGC 1 cut(s) 52
Bsh1236I CGCG 1 cut(s) 21
Bsh1285I CGRYCG 1 cut(s) 78
BshFI GGCC 5 cut(s) 5, 77, 205, 260, 368
BsiEI CGRYCG 1 cut(s) 78
BsiSI CCGG 3 cut(s) 6, 90, 241
BslI CCNNNNNNNGG 2 cut(s) 247, 375
BsmAI GTCTC 1 cut(s) 76
BsmBI CGTCTC 1 cut(s) 76
BsnI GGCC 5 cut(s) 5, 77, 205, 260, 368
Bsp120I GGGCCC 1 cut(s) 203
Bsp1286I GDGCHC 1 cut(s) 207
BspACI CCGC 8 cut(s) 19, 78, 212, 248, 257, 267, 270, 338
BspANI GGCC 5 cut(s) 5, 77, 205, 260, 368
BspFNI CGCG 1 cut(s) 21
BspLI GGNNCC 3 cut(s) 59, 205, 324
BsrFI RCCGGY 1 cut(s) 5
BsrI ACTGG 1 cut(s) 394
BssAI RCCGGY 1 cut(s) 5
BssECI CCNNGG 2 cut(s) 192, 207
BssNI GRCGYC 1 cut(s) 123
Bst2UI CCWGG 1 cut(s) 370
Bst6I CTCTTC 2 cut(s) 174, 227
BstACI GRCGYC 1 cut(s) 123
BstC8I GCNNGC 3 cut(s) 23, 255, 336
BstDEI CTNAG 1 cut(s) 357
BstFNI CGCG 1 cut(s) 21
BstH2I RGCGCY 2 cut(s) 53, 276
BstHHI GCGC 2 cut(s) 52, 275
BstMAI GTCTC 1 cut(s) 76
BstMCI CGRYCG 1 cut(s) 78
BstMWI GCNNNNNNNGC 2 cut(s) 86, 254
BstNI CCWGG 1 cut(s) 370
BstSCI CCNGG 1 cut(s) 368
BstSLI GKGCMC 1 cut(s) 207
BstUI CGCG 1 cut(s) 21
BstZI CGGCCG 1 cut(s) 75
BsuRI GGCC 5 cut(s) 5, 77, 205, 260, 368
BtgZI GCGATG 1 cut(s) 70
BtsI GCAGTG 1 cut(s) 61
BtsIMutI CAGTG 1 cut(s) 61
Cac8I GCNNGC 3 cut(s) 23, 255, 336
CfoI GCGC 2 cut(s) 52, 275
Cfr10I RCCGGY 1 cut(s) 5
Cfr13I GGNCC 3 cut(s) 58, 203, 204
Csp6I GTAC 1 cut(s) 37
CviQI GTAC 1 cut(s) 37
DdeI CTNAG 1 cut(s) 357
DriI GACNNNNNGTC 2 cut(s) 98, 127
EaeI YGGCCR 4 cut(s) 3, 75, 258, 366
EagI CGGCCG 1 cut(s) 75
Eam1104I CTCTTC 2 cut(s) 174, 227
Eam1105I GACNNNNNGTC 2 cut(s) 98, 127
EarI CTCTTC 2 cut(s) 174, 227
EciI GGCGGA 1 cut(s) 263
EclXI CGGCCG 1 cut(s) 75
Eco24I GRGCYC 1 cut(s) 207
Eco47I GGWCC 1 cut(s) 58
Eco52I CGGCCG 1 cut(s) 75
EcoO109I RGGNCCY 1 cut(s) 204
EcoRII CCWGG 1 cut(s) 368
EcoT38I GRGCYC 1 cut(s) 207
Esp3I CGTCTC 1 cut(s) 76
FaiI YATR 1 cut(s) 397
FblI GTMKAC 1 cut(s) 120
Fnu4HI GCNGC 5 cut(s) 78, 213, 258, 268, 271
FriOI GRGCYC 1 cut(s) 207
Fsp4HI GCNGC 5 cut(s) 78, 213, 258, 268, 271
GlaI GCGC 2 cut(s) 51, 274
GluI GCNGC 5 cut(s) 78, 213, 258, 268, 271
GsaI CCCAGC 1 cut(s) 56
HaeII RGCGCY 2 cut(s) 53, 276
HaeIII GGCC 5 cut(s) 5, 77, 205, 260, 368
HapII CCGG 3 cut(s) 6, 90, 241
HhaI GCGC 2 cut(s) 52, 275
Hin1I GRCGYC 1 cut(s) 123
Hin6I GCGC 2 cut(s) 50, 273
HinP1I GCGC 2 cut(s) 50, 273
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HinfI GANTC 3 cut(s) 93, 128, 316
HpaII CCGG 3 cut(s) 6, 90, 241
Hpy166II GTNNAC 2 cut(s) 61, 121
Hpy188I TCNGA 3 cut(s) 196, 315, 327
Hpy188III TCNNGA 2 cut(s) 109, 385
Hpy8I GTNNAC 2 cut(s) 61, 121
Hpy99I CGWCG 4 cut(s) 104, 122, 125, 128
HpyCH4IV ACGT 3 cut(s) 99, 117, 123
HpyCH4V TGCA 1 cut(s) 297
HpyF10VI GCNNNNNNNGC 2 cut(s) 86, 254
HpyF3I CTNAG 1 cut(s) 357
HpySE526I ACGT 3 cut(s) 99, 117, 123
Hsp92I GRCGYC 1 cut(s) 123
HspAI GCGC 2 cut(s) 50, 273
LmnI GCTCC 3 cut(s) 71, 150, 250
MaeII ACGT 3 cut(s) 99, 117, 123
MaeIII GTNAC 1 cut(s) 340
MboII GAAGA 4 cut(s) 43, 191, 241, 244
MhlI GDGCHC 1 cut(s) 207
MlsI TGGCCA 1 cut(s) 368
MluNI TGGCCA 1 cut(s) 368
MlyI GAGTC 2 cut(s) 87, 137
MnlI CCTC 9 cut(s) 91, 171, 181, 202, 217, 217, 357, 366, 369
Mox20I TGGCCA 1 cut(s) 368
MscI TGGCCA 1 cut(s) 368
MseI TTAA 1 cut(s) 165
Msp20I TGGCCA 1 cut(s) 368
MspI CCGG 3 cut(s) 6, 90, 241
MspR9I CCNGG 1 cut(s) 370
MvaI CCWGG 1 cut(s) 370
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 2 cut(s) 86, 254
NlaIV GGNNCC 3 cut(s) 59, 205, 324
NmeAIII GCCGAG 2 cut(s) 53, 188
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PfeI GAWTC 1 cut(s) 316
PkrI GCNGC 5 cut(s) 79, 214, 259, 269, 272
PleI GAGTC 2 cut(s) 87, 136
PpsI GAGTC 2 cut(s) 87, 136
Psp6I CCWGG 1 cut(s) 368
PspFI CCCAGC 1 cut(s) 52
PspGI CCWGG 1 cut(s) 368
PspN4I GGNNCC 3 cut(s) 59, 205, 324
PspOMI GGGCCC 1 cut(s) 203
PspPI GGNCC 3 cut(s) 58, 203, 204
RsaI GTAC 1 cut(s) 38
RsaNI GTAC 1 cut(s) 37
SalI GTCGAC 1 cut(s) 119
SaqAI TTAA 1 cut(s) 165
SatI GCNGC 5 cut(s) 78, 213, 258, 268, 271
Sau96I GGNCC 3 cut(s) 58, 203, 204
SchI GAGTC 2 cut(s) 87, 137
ScrFI CCNGG 1 cut(s) 370
SduI GDGCHC 1 cut(s) 207
SetI ASST 9 cut(s) 27, 48, 102, 120, 126, 147, 156, 194, 255
SgrDI CGTCGACG 1 cut(s) 119
SinI GGWCC 1 cut(s) 58
SsiI CCGC 8 cut(s) 19, 78, 212, 248, 257, 267, 270, 338
StyD4I CCNGG 1 cut(s) 368
TaiI ACGT 3 cut(s) 102, 120, 126
TaqI TCGA 2 cut(s) 120, 126
TauI GCSGC 5 cut(s) 80, 215, 260, 270, 273
TfiI GAWTC 1 cut(s) 316
Tru1I TTAA 1 cut(s) 165
Tru9I TTAA 1 cut(s) 165
TscAI CASTG 1 cut(s) 68
TspGWI ACGGA 1 cut(s) 58
TspRI CASTG 1 cut(s) 68
VpaK11BI GGWCC 1 cut(s) 58
XmiI GTMKAC 1 cut(s) 120
ZraI GACGTC 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.