MD10G1125900.v1.1

Protein of unknown function (DUF1279)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010849
Physical Location & Seq
Forward (+)
20598517 .. 20600491
1975 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1125900.v1.1.491

Sequence Viewer

Length: 456 bp
ATGGCCTCCGTCGCCACCGGCGGTCGATTCAAAGAGCTCCTGAAGAAGTACGGCAAGGTCGCACTTGGCGTCCATTTCTCAGTCTCAGCTGCCTCGATCACAGGCCTCTATGTCGCCATCAAGAACAACGTCGACGTCGAATCGTTGCTGGACAAGCTTCACATGGGGAGCGTGTCCTCCAAAGACGAAAACCCAGAGAACCCACCTGTGATCTCCGATGGGTCGGAGTTTCGGGACGGACCCATATCGGAAAATGGGCAATCGACGGCTGTGGTTGTGGAGAAGGAGAGGAGTCGGACGGCTGAGCTCGCGGCTTCGACTGGTGGTGCTCTGGCATTGGCTTTGCTCTGCAACAAGGCCCTGTTTCCGATTCGGGTTCCTATTACTCTGGCGCTTACTCCTCCCGTGTCTAGGTTTTTGGCCAGGAGAAGGCTCATCAAGACCGGCGGTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1279

Protein Analysis

152

Amino Acids

15.95

Weight (kDa)

9.56

Isoelectric Point (pI)

33.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAM210A-B_dom PF06916 9 - 134 6.8e-27 FAM210A/B-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 138
AccI GTMKAC 1 cut(s) 132
AccII CGCG 1 cut(s) 311
AciI CCGC 3 cut(s) 21, 311, 447
AcoI YGGCCR 1 cut(s) 420
AcuI CTGAAG 1 cut(s) 62
AcyI GRCGYC 2 cut(s) 69, 135
AfaI GTAC 1 cut(s) 50
AfiI CCNNNNNNNGG 2 cut(s) 411, 429
AgsI TTSAA 1 cut(s) 31
AhdI GACNNNNNGTC 1 cut(s) 447
AjnI CCWGG 1 cut(s) 422
AluBI AGCT 4 cut(s) 37, 89, 157, 307
AluI AGCT 4 cut(s) 37, 89, 157, 307
Alw21I GWGCWC 3 cut(s) 39, 309, 331
Alw26I GTCTC 1 cut(s) 88
AoxI GGCC 4 cut(s) 3, 103, 357, 420
ApeKI GCWGC 1 cut(s) 89
AspLEI GCGC 1 cut(s) 394
AspS9I GGNCC 2 cut(s) 239, 358
AvaII GGWCC 1 cut(s) 239
BalI TGGCCA 1 cut(s) 422
BanII GRGCYC 2 cut(s) 39, 309
Bbv12I GWGCWC 3 cut(s) 39, 309, 331
BbvI GCAGC 1 cut(s) 76
BccI CCATC 2 cut(s) 125, 212
BceAI ACGGC 3 cut(s) 67, 282, 315
BciT130I CCWGG 1 cut(s) 424
BcoDI GTCTC 1 cut(s) 88
BfaI CTAG 1 cut(s) 411
BfoI RGCGCY 1 cut(s) 395
BisI GCNGC 2 cut(s) 90, 312
BlpI GCTNAGC 1 cut(s) 303
BlsI GCNGC 2 cut(s) 91, 313
Bme1390I CCNGG 1 cut(s) 424
Bme18I GGWCC 1 cut(s) 239
BmeRI GACNNNNNGTC 1 cut(s) 447
BmgT120I GGNCC 2 cut(s) 239, 358
BmiI GGNNCC 2 cut(s) 241, 378
BmrFI CCNGG 1 cut(s) 424
Bpu1102I GCTNAGC 1 cut(s) 303
BsaHI GRCGYC 2 cut(s) 69, 135
Bsc4I CCNNNNNNNGG 2 cut(s) 411, 429
Bse118I RCCGGY 2 cut(s) 17, 443
Bse1I ACTGG 1 cut(s) 325
BseBI CCWGG 1 cut(s) 424
BseLI CCNNNNNNNGG 2 cut(s) 411, 429
BseMII CTCAG 3 cut(s) 93, 99, 294
BseNI ACTGG 1 cut(s) 325
BseRI GAGGAG 2 cut(s) 304, 390
BseXI GCAGC 1 cut(s) 76
Bsh1236I CGCG 1 cut(s) 311
Bsh1285I CGRYCG 1 cut(s) 25
BshFI GGCC 4 cut(s) 5, 105, 359, 422
BsiEI CGRYCG 1 cut(s) 25
BsiHKAI GWGCWC 3 cut(s) 39, 309, 331
BsiSI CCGG 2 cut(s) 18, 444
BslFI GGGAC 1 cut(s) 248
BslI CCNNNNNNNGG 2 cut(s) 411, 429
BsmAI GTCTC 1 cut(s) 88
BsmFI GGGAC 1 cut(s) 248
BsnI GGCC 4 cut(s) 5, 105, 359, 422
Bsp1286I GDGCHC 3 cut(s) 39, 309, 331
Bsp143I GATC 2 cut(s) 96, 210
Bsp1720I GCTNAGC 1 cut(s) 303
BspACI CCGC 3 cut(s) 21, 311, 447
BspANI GGCC 4 cut(s) 5, 105, 359, 422
BspCNI CTCAG 3 cut(s) 92, 98, 295
BspFNI CGCG 1 cut(s) 311
BspLI GGNNCC 2 cut(s) 241, 378
BsrFI RCCGGY 2 cut(s) 17, 443
BsrI ACTGG 1 cut(s) 325
BssAI RCCGGY 2 cut(s) 17, 443
BssMI GATC 2 cut(s) 96, 210
BssNI GRCGYC 2 cut(s) 69, 135
Bst2UI CCWGG 1 cut(s) 424
BstACI GRCGYC 2 cut(s) 69, 135
BstC8I GCNNGC 1 cut(s) 309
BstDEI CTNAG 3 cut(s) 79, 85, 303
BstFNI CGCG 1 cut(s) 311
BstH2I RGCGCY 1 cut(s) 395
BstHHI GCGC 1 cut(s) 394
BstKTI GATC 2 cut(s) 99, 213
BstMAI GTCTC 1 cut(s) 88
BstMBI GATC 2 cut(s) 96, 210
BstMCI CGRYCG 1 cut(s) 25
BstMWI GCNNNNNNNGC 3 cut(s) 11, 154, 308
BstNI CCWGG 1 cut(s) 424
BstSCI CCNGG 1 cut(s) 422
BstUI CGCG 1 cut(s) 311
BstV1I GCAGC 1 cut(s) 76
BsuRI GGCC 4 cut(s) 5, 105, 359, 422
Cac8I GCNNGC 1 cut(s) 309
CfoI GCGC 1 cut(s) 394
Cfr10I RCCGGY 2 cut(s) 17, 443
Cfr13I GGNCC 2 cut(s) 239, 358
CseI GACGC 1 cut(s) 58
Csp6I GTAC 1 cut(s) 49
CviAII CATG 1 cut(s) 163
CviQI GTAC 1 cut(s) 49
DdeI CTNAG 3 cut(s) 79, 85, 303
DpnI GATC 2 cut(s) 98, 212
DpnII GATC 2 cut(s) 96, 210
DriI GACNNNNNGTC 1 cut(s) 447
EaeI YGGCCR 1 cut(s) 420
Eam1105I GACNNNNNGTC 1 cut(s) 447
Ecl136II GAGCTC 2 cut(s) 37, 307
Eco147I AGGCCT 1 cut(s) 105
Eco24I GRGCYC 2 cut(s) 39, 309
Eco47I GGWCC 1 cut(s) 239
Eco53kI GAGCTC 2 cut(s) 37, 307
Eco57I CTGAAG 1 cut(s) 62
EcoICRI GAGCTC 2 cut(s) 37, 307
EcoO109I RGGNCCY 1 cut(s) 358
EcoRII CCWGG 1 cut(s) 422
EcoT38I GRGCYC 2 cut(s) 39, 309
FaeI CATG 1 cut(s) 166
FaiI YATR 4 cut(s) 111, 164, 245, 454
FaqI GGGAC 1 cut(s) 248
FatI CATG 1 cut(s) 162
FblI GTMKAC 1 cut(s) 132
Fnu4HI GCNGC 2 cut(s) 90, 312
FriOI GRGCYC 2 cut(s) 39, 309
Fsp4HI GCNGC 2 cut(s) 90, 312
FspBI CTAG 1 cut(s) 411
GlaI GCGC 1 cut(s) 393
GluI GCNGC 2 cut(s) 90, 312
HaeII RGCGCY 1 cut(s) 395
HaeIII GGCC 4 cut(s) 5, 105, 359, 422
HapII CCGG 2 cut(s) 18, 444
HgaI GACGC 1 cut(s) 58
HhaI GCGC 1 cut(s) 394
Hin1I GRCGYC 2 cut(s) 69, 135
Hin1II CATG 1 cut(s) 166
Hin6I GCGC 1 cut(s) 392
HinP1I GCGC 1 cut(s) 392
HincII GTYRAC 1 cut(s) 133
HindII GTYRAC 1 cut(s) 133
HindIII AAGCTT 1 cut(s) 155
HinfI GANTC 4 cut(s) 27, 140, 292, 370
HpaII CCGG 2 cut(s) 18, 444
Hpy166II GTNNAC 1 cut(s) 133
Hpy188I TCNGA 5 cut(s) 217, 226, 250, 297, 369
Hpy188III TCNNGA 4 cut(s) 40, 121, 233, 439
Hpy8I GTNNAC 1 cut(s) 133
Hpy99I CGWCG 5 cut(s) 14, 134, 137, 140, 268
HpyAV CCTTC 2 cut(s) 277, 423
HpyCH4IV ACGT 2 cut(s) 129, 135
HpyCH4V TGCA 1 cut(s) 351
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 154, 308
HpyF3I CTNAG 3 cut(s) 79, 85, 303
HpySE526I ACGT 2 cut(s) 129, 135
Hsp92I GRCGYC 2 cut(s) 69, 135
Hsp92II CATG 1 cut(s) 166
HspAI GCGC 1 cut(s) 392
Kzo9I GATC 2 cut(s) 96, 210
LmnI GCTCC 2 cut(s) 42, 168
Lsp1109I GCAGC 1 cut(s) 76
MaeI CTAG 1 cut(s) 411
MaeII ACGT 2 cut(s) 129, 135
MalI GATC 2 cut(s) 98, 212
MboI GATC 2 cut(s) 96, 210
MboII GAAGA 1 cut(s) 55
MhlI GDGCHC 3 cut(s) 39, 309, 331
MlsI TGGCCA 1 cut(s) 422
MluNI TGGCCA 1 cut(s) 422
MlyI GAGTC 1 cut(s) 301
MmeI TCCRAC 2 cut(s) 204, 275
MnlI CCTC 6 cut(s) 16, 103, 116, 187, 282, 411
Mox20I TGGCCA 1 cut(s) 422
MscI TGGCCA 1 cut(s) 422
Msp20I TGGCCA 1 cut(s) 422
MspA1I CMGCKG 1 cut(s) 89
MspI CCGG 2 cut(s) 18, 444
MspR9I CCNGG 1 cut(s) 424
MvaI CCWGG 1 cut(s) 424
MvnI CGCG 1 cut(s) 311
MwoI GCNNNNNNNGC 3 cut(s) 11, 154, 308
NdeII GATC 2 cut(s) 96, 210
NlaIII CATG 1 cut(s) 166
NlaIV GGNNCC 2 cut(s) 241, 378
PceI AGGCCT 1 cut(s) 105
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 135
PfeI GAWTC 3 cut(s) 27, 140, 370
PkrI GCNGC 2 cut(s) 91, 313
PleI GAGTC 1 cut(s) 300
PpsI GAGTC 1 cut(s) 300
Psp124BI GAGCTC 2 cut(s) 39, 309
Psp6I CCWGG 1 cut(s) 422
PspGI CCWGG 1 cut(s) 422
PspN4I GGNNCC 2 cut(s) 241, 378
PspPI GGNCC 2 cut(s) 239, 358
PvuII CAGCTG 1 cut(s) 89
RsaI GTAC 1 cut(s) 50
RsaNI GTAC 1 cut(s) 49
SacI GAGCTC 2 cut(s) 39, 309
SalI GTCGAC 1 cut(s) 131
SatI GCNGC 2 cut(s) 90, 312
Sau3AI GATC 2 cut(s) 96, 210
Sau96I GGNCC 2 cut(s) 239, 358
SchI GAGTC 1 cut(s) 301
ScrFI CCNGG 1 cut(s) 424
SduI GDGCHC 3 cut(s) 39, 309, 331
SetI ASST 9 cut(s) 39, 60, 91, 132, 138, 159, 208, 309, 416
SgrAI CRCCGGYG 1 cut(s) 17
SgrDI CGTCGACG 1 cut(s) 131
SinI GGWCC 1 cut(s) 239
SseBI AGGCCT 1 cut(s) 105
SsiI CCGC 3 cut(s) 21, 311, 447
SspMI CTAG 1 cut(s) 411
SstI GAGCTC 2 cut(s) 39, 309
StuI AGGCCT 1 cut(s) 105
StyD4I CCNGG 1 cut(s) 422
TaiI ACGT 2 cut(s) 132, 138
TaqI TCGA 6 cut(s) 25, 95, 132, 138, 263, 317
TauI GCSGC 1 cut(s) 314
TfiI GAWTC 3 cut(s) 27, 140, 370
TseI GCWGC 1 cut(s) 89
TspGWI ACGGA 1 cut(s) 252
VpaK11BI GGWCC 1 cut(s) 239
XmiI GTMKAC 1 cut(s) 132
XspI CTAG 1 cut(s) 411
ZraI GACGTC 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.