MD05G1195600.v1.1

Protein of unknown function (DUF1279)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010849
Physical Location & Seq
Reverse (-)
32304017 .. 32304430
414 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1195600.v1.1.491

Sequence Viewer

Length: 414 bp
ATGGCCCTCGTCGCCACCCGCGATCGATTCAAAGAGCTTCTGAATAAGTACGGTAAGGTCGCACTAGGCGTCCATTTCTCAGTCTCAACTGCCTCGATCGCCAGCCTCTATGTCGCCATCAAGAGCAACGTCGGCGTCGAATCACTGCTCGCCAAGCTTCACATGGGGAACGTGTCCTTCAAAGACGAAACCTCCCCAAGCTTCACATTGGGAAACAGGCAATCGACGGCTGTGGTGGTGGAGAATGAGAGGAATCGAACGGCTGAGCTCACGGCTTTGACTGGTGGGGCTCTGACATTGGCTTTGCTCTGCAACAAGGCTTTACTTCCAATTCGAGTTCCGAACCCTCTGTTCATCAAGACTGGCGAACTGAGGACGAAGCAAGCGGGGAGAGGATTGTGCCGTGGAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1279

Protein Analysis

138

Amino Acids

14.67

Weight (kDa)

10.09

Isoelectric Point (pI)

19.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAM210A-B_dom PF06916 9 - 114 1.6e-20 FAM210A/B-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 21
AciI CCGC 2 cut(s) 19, 386
AcyI GRCGYC 2 cut(s) 69, 135
AfaI GTAC 1 cut(s) 50
AflIII ACRYGT 1 cut(s) 171
AgsI TTSAA 2 cut(s) 31, 181
AluBI AGCT 4 cut(s) 37, 157, 201, 268
AluI AGCT 4 cut(s) 37, 157, 201, 268
Alw21I GWGCWC 1 cut(s) 270
Alw26I GTCTC 1 cut(s) 88
AoxI GGCC 1 cut(s) 3
ArsI GACNNNNNNTTYG 2 cut(s) 286, 318
AspS9I GGNCC 1 cut(s) 4
BanII GRGCYC 2 cut(s) 270, 292
Bbv12I GWGCWC 1 cut(s) 270
BccI CCATC 1 cut(s) 125
BceAI ACGGC 4 cut(s) 243, 276, 288, 387
BcoDI GTCTC 1 cut(s) 88
BfaI CTAG 1 cut(s) 65
BlpI GCTNAGC 1 cut(s) 264
BmgT120I GGNCC 1 cut(s) 4
Bpu1102I GCTNAGC 1 cut(s) 264
Bsa29I ATCGAT 1 cut(s) 25
BsaHI GRCGYC 2 cut(s) 69, 135
BsaJI CCNNGG 1 cut(s) 403
BsaXI ACNNNNNCTCC 2 cut(s) 176, 206
Bse1I ACTGG 2 cut(s) 286, 367
BseCI ATCGAT 1 cut(s) 25
BseDI CCNNGG 1 cut(s) 403
BseMII CTCAG 3 cut(s) 93, 255, 362
BseNI ACTGG 2 cut(s) 286, 367
Bsh1236I CGCG 1 cut(s) 21
Bsh1285I CGRYCG 2 cut(s) 25, 99
BshFI GGCC 1 cut(s) 5
BshVI ATCGAT 1 cut(s) 25
BsiEI CGRYCG 2 cut(s) 25, 99
BsiHKAI GWGCWC 1 cut(s) 270
BsmAI GTCTC 1 cut(s) 88
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 2 cut(s) 270, 292
Bsp143I GATC 2 cut(s) 22, 96
Bsp1720I GCTNAGC 1 cut(s) 264
BspACI CCGC 2 cut(s) 19, 386
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 3 cut(s) 92, 256, 363
BspDI ATCGAT 1 cut(s) 25
BspFNI CGCG 1 cut(s) 21
BsrI ACTGG 2 cut(s) 286, 367
BssECI CCNNGG 1 cut(s) 403
BssMI GATC 2 cut(s) 22, 96
BssNI GRCGYC 2 cut(s) 69, 135
Bst4CI ACNGT 1 cut(s) 53
BstACI GRCGYC 2 cut(s) 69, 135
BstC8I GCNNGC 3 cut(s) 103, 150, 384
BstDEI CTNAG 3 cut(s) 79, 264, 371
BstDSI CCRYGG 1 cut(s) 403
BstFNI CGCG 1 cut(s) 21
BstKTI GATC 2 cut(s) 25, 99
BstMAI GTCTC 1 cut(s) 88
BstMBI GATC 2 cut(s) 22, 96
BstMCI CGRYCG 2 cut(s) 25, 99
BstMWI GCNNNNNNNGC 4 cut(s) 11, 98, 132, 154
BstUI CGCG 1 cut(s) 21
Bsu15I ATCGAT 1 cut(s) 25
BsuRI GGCC 1 cut(s) 5
BsuTUI ATCGAT 1 cut(s) 25
BtgI CCRYGG 1 cut(s) 403
BtsI GCAGTG 1 cut(s) 143
BtsIMutI CAGTG 1 cut(s) 143
Cac8I GCNNGC 3 cut(s) 103, 150, 384
Cfr13I GGNCC 1 cut(s) 4
ClaI ATCGAT 1 cut(s) 25
CseI GACGC 2 cut(s) 58, 124
Csp6I GTAC 1 cut(s) 49
CviAII CATG 1 cut(s) 163
CviQI GTAC 1 cut(s) 49
DdeI CTNAG 3 cut(s) 79, 264, 371
DpnI GATC 2 cut(s) 24, 98
DpnII GATC 2 cut(s) 22, 96
Ecl136II GAGCTC 1 cut(s) 268
Eco24I GRGCYC 2 cut(s) 270, 292
Eco53kI GAGCTC 1 cut(s) 268
EcoICRI GAGCTC 1 cut(s) 268
EcoT38I GRGCYC 2 cut(s) 270, 292
FaeI CATG 1 cut(s) 166
FaiI YATR 2 cut(s) 111, 164
FatI CATG 1 cut(s) 162
FauI CCCGC 2 cut(s) 26, 379
FriOI GRGCYC 2 cut(s) 270, 292
FspBI CTAG 1 cut(s) 65
HaeIII GGCC 1 cut(s) 5
HgaI GACGC 2 cut(s) 58, 124
Hin1I GRCGYC 2 cut(s) 69, 135
Hin1II CATG 1 cut(s) 166
HindIII AAGCTT 2 cut(s) 155, 199
HinfI GANTC 3 cut(s) 27, 140, 253
Hpy188I TCNGA 3 cut(s) 42, 294, 342
Hpy188III TCNNGA 2 cut(s) 121, 358
Hpy99I CGWCG 4 cut(s) 14, 134, 140, 229
HpyAV CCTTC 1 cut(s) 187
HpyCH4III ACNGT 1 cut(s) 53
HpyCH4IV ACGT 2 cut(s) 129, 171
HpyCH4V TGCA 1 cut(s) 312
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 98, 132, 154
HpyF3I CTNAG 3 cut(s) 79, 264, 371
HpySE526I ACGT 2 cut(s) 129, 171
Hsp92I GRCGYC 2 cut(s) 69, 135
Hsp92II CATG 1 cut(s) 166
Kzo9I GATC 2 cut(s) 22, 96
LpnPI CCDG 4 cut(s) 115, 202, 267, 348
MaeI CTAG 1 cut(s) 65
MaeII ACGT 2 cut(s) 129, 171
MalI GATC 2 cut(s) 24, 98
MboI GATC 2 cut(s) 22, 96
MhlI GDGCHC 2 cut(s) 270, 292
MluCI AATT 1 cut(s) 330
MnlI CCTC 8 cut(s) 17, 103, 116, 202, 243, 357, 366, 386
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 4 cut(s) 11, 98, 132, 154
NdeII GATC 2 cut(s) 22, 96
NlaIII CATG 1 cut(s) 166
PcsI WCGNNNNNNNCGW 3 cut(s) 18, 66, 135
PfeI GAWTC 3 cut(s) 27, 140, 253
Ple19I CGATCG 2 cut(s) 25, 99
Psp124BI GAGCTC 1 cut(s) 270
PspPI GGNCC 1 cut(s) 4
PvuI CGATCG 2 cut(s) 25, 99
RsaI GTAC 1 cut(s) 50
RsaNI GTAC 1 cut(s) 49
SacI GAGCTC 1 cut(s) 270
Sau3AI GATC 2 cut(s) 22, 96
Sau96I GGNCC 1 cut(s) 4
SduI GDGCHC 2 cut(s) 270, 292
SetI ASST 8 cut(s) 39, 60, 132, 159, 174, 194, 203, 270
Sse9I AATT 1 cut(s) 330
SsiI CCGC 2 cut(s) 19, 386
SspMI CTAG 1 cut(s) 65
SstI GAGCTC 1 cut(s) 270
TaaI ACNGT 1 cut(s) 53
TaiI ACGT 2 cut(s) 132, 174
TaqI TCGA 6 cut(s) 25, 95, 138, 224, 256, 334
TasI AATT 1 cut(s) 330
TfiI GAWTC 3 cut(s) 27, 140, 253
TscAI CASTG 1 cut(s) 150
TspDTI ATGAA 1 cut(s) 343
TspRI CASTG 1 cut(s) 150
XcmI CCANNNNNNNNNTGG 1 cut(s) 160
XspI CTAG 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.