Rroxscaffold_7G00189860

Protein of unknown function (DUF1279)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010849
Physical Location & Seq
Reverse (-)
29685014 .. 29685409
396 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00189860.1

Sequence Viewer

Length: 396 bp
ATGGCCGGTGGTCGATTCCGCGAGCTTCTGAAGAAGTACGGCAAGGTCGCGCTGGGCGTCCACTGCTCCGTCTCCGCCGCCTCCGTCGCCGGACTCTACGTCGCCATCAAGAACAACGTCGATGTGGAGTCGCTCATCGACAAGCTTCCCAATCTCAACCCTCTTAAAAACTCTCATGAAGAGAAACCCACCTCCGAAAATGGGCCTTCGGCTGTGGTTGAGGAGAGGAAGAGGAATCGGACGGCTGAGCTCGCAGCCACCAGCGGCGGTGCTCTGGCATTGGCTTTTCTCTGCAACAAGGCTCTGTTTCCGATTCGGGTTCCGATTACGCTGGCGGTCACGCCTCCTTTGGCCAAGTTTTTGGCCAGGAGGAGGCTTATCAAGACCGGTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1279

Protein Analysis

131

Amino Acids

14.03

Weight (kDa)

10.07

Isoelectric Point (pI)

31.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAM210A-B_dom PF06916 5 - 115 1.3e-23 FAM210A/B-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 21, 50
AciI CCGC 6 cut(s) 19, 75, 78, 264, 267, 335
AcoI YGGCCR 3 cut(s) 3, 351, 363
AcuI CTGAAG 1 cut(s) 50
AcyI GRCGYC 1 cut(s) 57
AfaI GTAC 1 cut(s) 38
AfiI CCNNNNNNNGG 2 cut(s) 201, 372
AgeI ACCGGT 1 cut(s) 386
AhdI GACNNNNNGTC 1 cut(s) 98
AjnI CCWGG 1 cut(s) 365
AluBI AGCT 3 cut(s) 25, 145, 250
AluI AGCT 3 cut(s) 25, 145, 250
Alw21I GWGCWC 2 cut(s) 252, 274
Alw26I GTCTC 1 cut(s) 76
AoxI GGCC 4 cut(s) 3, 203, 351, 363
ApeKI GCWGC 1 cut(s) 254
AsiGI ACCGGT 1 cut(s) 386
AspLEI GCGC 1 cut(s) 52
AspS9I GGNCC 1 cut(s) 203
BalI TGGCCA 2 cut(s) 353, 365
BanII GRGCYC 1 cut(s) 252
Bbv12I GWGCWC 2 cut(s) 252, 274
BbvI GCAGC 1 cut(s) 266
BccI CCATC 1 cut(s) 113
BceAI ACGGC 2 cut(s) 55, 258
BciT130I CCWGG 1 cut(s) 367
BcoDI GTCTC 1 cut(s) 76
BisI GCNGC 3 cut(s) 78, 255, 265
BlpI GCTNAGC 1 cut(s) 246
BlsI GCNGC 3 cut(s) 79, 256, 266
Bme1390I CCNGG 1 cut(s) 367
BmeRI GACNNNNNGTC 1 cut(s) 98
BmgT120I GGNCC 1 cut(s) 203
BmiI GGNNCC 1 cut(s) 321
BmrFI CCNGG 1 cut(s) 367
Bpu1102I GCTNAGC 1 cut(s) 246
BsaHI GRCGYC 1 cut(s) 57
BsaWI WCCGGW 1 cut(s) 386
Bsc4I CCNNNNNNNGG 2 cut(s) 201, 372
Bse118I RCCGGY 2 cut(s) 5, 386
BseBI CCWGG 1 cut(s) 367
BseLI CCNNNNNNNGG 2 cut(s) 201, 372
BseMII CTCAG 1 cut(s) 237
BseRI GAGGAG 2 cut(s) 236, 385
BseXI GCAGC 1 cut(s) 266
BseYI CCCAGC 1 cut(s) 52
Bsh1236I CGCG 2 cut(s) 21, 50
BshFI GGCC 4 cut(s) 5, 205, 353, 365
BshTI ACCGGT 1 cut(s) 386
BsiHKAI GWGCWC 2 cut(s) 252, 274
BsiSI CCGG 3 cut(s) 6, 90, 387
BslI CCNNNNNNNGG 2 cut(s) 201, 372
BsmAI GTCTC 1 cut(s) 76
BsmBI CGTCTC 1 cut(s) 76
BsnI GGCC 4 cut(s) 5, 205, 353, 365
Bsp1286I GDGCHC 2 cut(s) 252, 274
Bsp1720I GCTNAGC 1 cut(s) 246
BspACI CCGC 6 cut(s) 19, 75, 78, 264, 267, 335
BspANI GGCC 4 cut(s) 5, 205, 353, 365
BspCNI CTCAG 1 cut(s) 238
BspFNI CGCG 2 cut(s) 21, 50
BspHI TCATGA 1 cut(s) 175
BspLI GGNNCC 1 cut(s) 321
BsrFI RCCGGY 2 cut(s) 5, 386
BssAI RCCGGY 2 cut(s) 5, 386
BssNI GRCGYC 1 cut(s) 57
Bst2UI CCWGG 1 cut(s) 367
Bst6I CTCTTC 2 cut(s) 174, 224
BstACI GRCGYC 1 cut(s) 57
BstC8I GCNNGC 3 cut(s) 23, 252, 333
BstDEI CTNAG 1 cut(s) 246
BstFNI CGCG 2 cut(s) 21, 50
BstHHI GCGC 1 cut(s) 52
BstMAI GTCTC 1 cut(s) 76
BstMWI GCNNNNNNNGC 3 cut(s) 63, 86, 251
BstNI CCWGG 1 cut(s) 367
BstSCI CCNGG 1 cut(s) 365
BstUI CGCG 2 cut(s) 21, 50
BstV1I GCAGC 1 cut(s) 266
BstXI CCANNNNNNTGG 1 cut(s) 361
BsuRI GGCC 4 cut(s) 5, 205, 353, 365
BtsI GCAGTG 1 cut(s) 61
BtsIMutI CAGTG 1 cut(s) 61
Cac8I GCNNGC 3 cut(s) 23, 252, 333
CciI TCATGA 1 cut(s) 175
CfoI GCGC 1 cut(s) 52
Cfr10I RCCGGY 2 cut(s) 5, 386
Cfr13I GGNCC 1 cut(s) 203
CseI GACGC 1 cut(s) 46
Csp6I GTAC 1 cut(s) 37
CspAI ACCGGT 1 cut(s) 386
CviAII CATG 1 cut(s) 176
CviQI GTAC 1 cut(s) 37
DdeI CTNAG 1 cut(s) 246
DriI GACNNNNNGTC 1 cut(s) 98
EaeI YGGCCR 3 cut(s) 3, 351, 363
Eam1104I CTCTTC 2 cut(s) 174, 224
Eam1105I GACNNNNNGTC 1 cut(s) 98
EarI CTCTTC 2 cut(s) 174, 224
EciI GGCGGA 1 cut(s) 64
Ecl136II GAGCTC 1 cut(s) 250
Eco24I GRGCYC 1 cut(s) 252
Eco53kI GAGCTC 1 cut(s) 250
Eco57I CTGAAG 1 cut(s) 50
EcoICRI GAGCTC 1 cut(s) 250
EcoRII CCWGG 1 cut(s) 365
EcoT38I GRGCYC 1 cut(s) 252
Esp3I CGTCTC 1 cut(s) 76
FaeI CATG 1 cut(s) 179
FaiI YATR 2 cut(s) 177, 394
FatI CATG 1 cut(s) 175
Fnu4HI GCNGC 3 cut(s) 78, 255, 265
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 3 cut(s) 78, 255, 265
GlaI GCGC 1 cut(s) 51
GluI GCNGC 3 cut(s) 78, 255, 265
GsaI CCCAGC 1 cut(s) 56
HaeIII GGCC 4 cut(s) 5, 205, 353, 365
HapII CCGG 3 cut(s) 6, 90, 387
HgaI GACGC 1 cut(s) 46
HhaI GCGC 1 cut(s) 52
Hin1I GRCGYC 1 cut(s) 57
Hin1II CATG 1 cut(s) 179
Hin6I GCGC 1 cut(s) 50
HinP1I GCGC 1 cut(s) 50
HindIII AAGCTT 1 cut(s) 143
HinfI GANTC 5 cut(s) 15, 93, 128, 235, 313
HpaII CCGG 3 cut(s) 6, 90, 387
Hpy166II GTNNAC 1 cut(s) 61
Hpy188I TCNGA 5 cut(s) 30, 196, 240, 312, 324
Hpy188III TCNNGA 3 cut(s) 109, 176, 382
Hpy8I GTNNAC 1 cut(s) 61
Hpy99I CGWCG 3 cut(s) 89, 104, 122
HpyAV CCTTC 1 cut(s) 216
HpyCH4IV ACGT 2 cut(s) 99, 117
HpyCH4V TGCA 1 cut(s) 294
HpyF10VI GCNNNNNNNGC 3 cut(s) 63, 86, 251
HpyF3I CTNAG 1 cut(s) 246
HpySE526I ACGT 2 cut(s) 99, 117
Hsp92I GRCGYC 1 cut(s) 57
Hsp92II CATG 1 cut(s) 179
HspAI GCGC 1 cut(s) 50
LmnI GCTCC 1 cut(s) 71
LpnPI CCDG 8 cut(s) 19, 38, 103, 260, 274, 317, 352, 379
Lsp1109I GCAGC 1 cut(s) 266
MaeII ACGT 2 cut(s) 99, 117
MaeIII GTNAC 1 cut(s) 337
MboII GAAGA 3 cut(s) 43, 191, 241
MhlI GDGCHC 2 cut(s) 252, 274
MlsI TGGCCA 2 cut(s) 353, 365
MluNI TGGCCA 2 cut(s) 353, 365
MlyI GAGTC 2 cut(s) 87, 137
MnlI CCTC 9 cut(s) 91, 171, 202, 214, 219, 225, 354, 363, 366
Mox20I TGGCCA 2 cut(s) 353, 365
MscI TGGCCA 2 cut(s) 353, 365
MseI TTAA 1 cut(s) 165
Msp20I TGGCCA 2 cut(s) 353, 365
MspA1I CMGCKG 1 cut(s) 264
MspI CCGG 3 cut(s) 6, 90, 387
MspR9I CCNGG 1 cut(s) 367
MvaI CCWGG 1 cut(s) 367
MvnI CGCG 2 cut(s) 21, 50
MwoI GCNNNNNNNGC 3 cut(s) 63, 86, 251
NlaIII CATG 1 cut(s) 179
NlaIV GGNNCC 1 cut(s) 321
NmuCI GTSAC 1 cut(s) 337
PagI TCATGA 1 cut(s) 175
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PfeI GAWTC 3 cut(s) 15, 235, 313
PinAI ACCGGT 1 cut(s) 386
PkrI GCNGC 3 cut(s) 79, 256, 266
PleI GAGTC 2 cut(s) 87, 136
PpsI GAGTC 2 cut(s) 87, 136
Psp124BI GAGCTC 1 cut(s) 252
Psp6I CCWGG 1 cut(s) 365
PspFI CCCAGC 1 cut(s) 52
PspGI CCWGG 1 cut(s) 365
PspN4I GGNNCC 1 cut(s) 321
PspPI GGNCC 1 cut(s) 203
RsaI GTAC 1 cut(s) 38
RsaNI GTAC 1 cut(s) 37
SacI GAGCTC 1 cut(s) 252
SaqAI TTAA 1 cut(s) 165
SatI GCNGC 3 cut(s) 78, 255, 265
Sau96I GGNCC 1 cut(s) 203
SchI GAGTC 2 cut(s) 87, 137
ScrFI CCNGG 1 cut(s) 367
SduI GDGCHC 2 cut(s) 252, 274
SetI ASST 7 cut(s) 27, 48, 102, 120, 147, 194, 252
SsiI CCGC 6 cut(s) 19, 75, 78, 264, 267, 335
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 1 cut(s) 365
TaiI ACGT 2 cut(s) 102, 120
TaqI TCGA 3 cut(s) 13, 120, 138
TauI GCSGC 2 cut(s) 80, 267
TfiI GAWTC 3 cut(s) 15, 235, 313
Tru1I TTAA 1 cut(s) 165
Tru9I TTAA 1 cut(s) 165
TscAI CASTG 1 cut(s) 68
TseFI GTSAC 1 cut(s) 337
TseI GCWGC 1 cut(s) 254
Tsp45I GTSAC 1 cut(s) 337
TspDTI ATGAA 1 cut(s) 192
TspGWI ACGGA 2 cut(s) 58, 73
TspRI CASTG 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.