MD05G1123100.v1.1

Protein of unknown function (DUF1279)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010849
Physical Location & Seq
Forward (+)
24572828 .. 24574581
1754 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1123100.v1.1.491

Sequence Viewer

Length: 456 bp
ATGGCCTCCGTCGCCACCGGCGGTCGATTCAAAGAGCTCCTGAAGAAGTACGGCAAGGTCGCACTGGGCGTCCATTTCTCAGTCTCAGCTGCCTCGATCACAGGCCTCTATGTCGCCATCAAGAACAACGTCGACGTCGAATCGCTGCTCGCCAAGCTTCACATGGGGAGTGTGTCCTCCAAAGACGAAACCCCCCAAAACCCACCTGTAATCTCCGATGGATCGGTGTTTCGGGACGGACCCACATCGGGAAACGGGCAATCGACGGCTGTGGTTGTGGAGAAGGAGAGGAATCGGACGGCTGAGCTCGCGGCTTCGACTGGTGGGGCTCTGGCATTGGCTTTGCTCTGCAACAAGGCTCTGTTTCCGATTCGGGTTCCGATCACTCTGGCGCTTACTCCTCCTGTGTCTAGGTTTTTGGCGCGAAGAAGGTTCATCAAGACCGGCGGTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1279

Protein Analysis

152

Amino Acids

15.83

Weight (kDa)

10.14

Isoelectric Point (pI)

27.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAM210A-B_dom PF06916 9 - 134 9e-27 FAM210A/B-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 138
AccI GTMKAC 1 cut(s) 132
AccII CGCG 2 cut(s) 311, 424
AciI CCGC 3 cut(s) 21, 311, 447
AclWI GGATC 1 cut(s) 229
AcuI CTGAAG 1 cut(s) 62
AcyI GRCGYC 2 cut(s) 69, 135
AfaI GTAC 1 cut(s) 50
AfiI CCNNNNNNNGG 1 cut(s) 248
AgsI TTSAA 1 cut(s) 31
AhdI GACNNNNNGTC 1 cut(s) 447
AluBI AGCT 4 cut(s) 37, 89, 157, 307
AluI AGCT 4 cut(s) 37, 89, 157, 307
Alw21I GWGCWC 2 cut(s) 39, 309
Alw26I GTCTC 1 cut(s) 88
AlwI GGATC 1 cut(s) 229
AoxI GGCC 2 cut(s) 3, 103
ApeKI GCWGC 2 cut(s) 89, 145
AspLEI GCGC 2 cut(s) 394, 424
AspS9I GGNCC 1 cut(s) 239
AvaII GGWCC 1 cut(s) 239
BanII GRGCYC 3 cut(s) 39, 309, 331
Bbv12I GWGCWC 2 cut(s) 39, 309
BbvI GCAGC 2 cut(s) 76, 132
BccI CCATC 2 cut(s) 125, 212
BceAI ACGGC 3 cut(s) 67, 282, 315
BcoDI GTCTC 1 cut(s) 88
BfaI CTAG 1 cut(s) 411
BfoI RGCGCY 1 cut(s) 395
BisI GCNGC 3 cut(s) 90, 146, 312
BlpI GCTNAGC 1 cut(s) 303
BlsI GCNGC 3 cut(s) 91, 147, 313
Bme18I GGWCC 1 cut(s) 239
BmeRI GACNNNNNGTC 1 cut(s) 447
BmgT120I GGNCC 1 cut(s) 239
BmiI GGNNCC 2 cut(s) 241, 378
BmrI ACTGGG 1 cut(s) 74
BmuI ACTGGG 1 cut(s) 74
Bpu1102I GCTNAGC 1 cut(s) 303
BsaHI GRCGYC 2 cut(s) 69, 135
Bsc4I CCNNNNNNNGG 1 cut(s) 248
Bse118I RCCGGY 2 cut(s) 17, 443
Bse1I ACTGG 2 cut(s) 69, 325
BseLI CCNNNNNNNGG 1 cut(s) 248
BseMII CTCAG 3 cut(s) 93, 99, 294
BseNI ACTGG 2 cut(s) 69, 325
BseRI GAGGAG 1 cut(s) 390
BseXI GCAGC 2 cut(s) 76, 132
Bsh1236I CGCG 2 cut(s) 311, 424
Bsh1285I CGRYCG 1 cut(s) 25
BshFI GGCC 2 cut(s) 5, 105
BsiEI CGRYCG 1 cut(s) 25
BsiHKAI GWGCWC 2 cut(s) 39, 309
BsiSI CCGG 2 cut(s) 18, 444
BslFI GGGAC 1 cut(s) 248
BslI CCNNNNNNNGG 1 cut(s) 248
BsmAI GTCTC 1 cut(s) 88
BsmFI GGGAC 1 cut(s) 248
BsnI GGCC 2 cut(s) 5, 105
Bsp1286I GDGCHC 3 cut(s) 39, 309, 331
Bsp143I GATC 3 cut(s) 96, 221, 381
Bsp1720I GCTNAGC 1 cut(s) 303
BspACI CCGC 3 cut(s) 21, 311, 447
BspANI GGCC 2 cut(s) 5, 105
BspCNI CTCAG 3 cut(s) 92, 98, 295
BspFNI CGCG 2 cut(s) 311, 424
BspLI GGNNCC 2 cut(s) 241, 378
BspPI GGATC 1 cut(s) 229
BsrFI RCCGGY 2 cut(s) 17, 443
BsrI ACTGG 2 cut(s) 69, 325
BssAI RCCGGY 2 cut(s) 17, 443
BssMI GATC 3 cut(s) 96, 221, 381
BssNI GRCGYC 2 cut(s) 69, 135
BstACI GRCGYC 2 cut(s) 69, 135
BstC8I GCNNGC 2 cut(s) 150, 309
BstDEI CTNAG 3 cut(s) 79, 85, 303
BstFNI CGCG 2 cut(s) 311, 424
BstH2I RGCGCY 1 cut(s) 395
BstHHI GCGC 2 cut(s) 394, 424
BstKTI GATC 3 cut(s) 99, 224, 384
BstMAI GTCTC 1 cut(s) 88
BstMBI GATC 3 cut(s) 96, 221, 381
BstMCI CGRYCG 1 cut(s) 25
BstMWI GCNNNNNNNGC 3 cut(s) 11, 154, 308
BstUI CGCG 2 cut(s) 311, 424
BstV1I GCAGC 2 cut(s) 76, 132
BsuRI GGCC 2 cut(s) 5, 105
BtsIMutI CAGTG 1 cut(s) 62
Cac8I GCNNGC 2 cut(s) 150, 309
CfoI GCGC 2 cut(s) 394, 424
Cfr10I RCCGGY 2 cut(s) 17, 443
Cfr13I GGNCC 1 cut(s) 239
CseI GACGC 1 cut(s) 58
Csp6I GTAC 1 cut(s) 49
CviAII CATG 1 cut(s) 163
CviQI GTAC 1 cut(s) 49
DdeI CTNAG 3 cut(s) 79, 85, 303
DpnI GATC 3 cut(s) 98, 223, 383
DpnII GATC 3 cut(s) 96, 221, 381
DriI GACNNNNNGTC 1 cut(s) 447
Eam1105I GACNNNNNGTC 1 cut(s) 447
Ecl136II GAGCTC 2 cut(s) 37, 307
Eco147I AGGCCT 1 cut(s) 105
Eco24I GRGCYC 3 cut(s) 39, 309, 331
Eco47I GGWCC 1 cut(s) 239
Eco53kI GAGCTC 2 cut(s) 37, 307
Eco57I CTGAAG 1 cut(s) 62
EcoICRI GAGCTC 2 cut(s) 37, 307
EcoT38I GRGCYC 3 cut(s) 39, 309, 331
FaeI CATG 1 cut(s) 166
FaiI YATR 3 cut(s) 111, 164, 454
FaqI GGGAC 1 cut(s) 248
FatI CATG 1 cut(s) 162
FblI GTMKAC 1 cut(s) 132
Fnu4HI GCNGC 3 cut(s) 90, 146, 312
FriOI GRGCYC 3 cut(s) 39, 309, 331
Fsp4HI GCNGC 3 cut(s) 90, 146, 312
FspBI CTAG 1 cut(s) 411
GlaI GCGC 2 cut(s) 393, 423
GluI GCNGC 3 cut(s) 90, 146, 312
HaeII RGCGCY 1 cut(s) 395
HaeIII GGCC 2 cut(s) 5, 105
HapII CCGG 2 cut(s) 18, 444
HgaI GACGC 1 cut(s) 58
HhaI GCGC 2 cut(s) 394, 424
Hin1I GRCGYC 2 cut(s) 69, 135
Hin1II CATG 1 cut(s) 166
Hin6I GCGC 2 cut(s) 392, 422
HinP1I GCGC 2 cut(s) 392, 422
HincII GTYRAC 1 cut(s) 133
HindII GTYRAC 1 cut(s) 133
HindIII AAGCTT 1 cut(s) 155
HinfI GANTC 4 cut(s) 27, 140, 292, 370
HpaII CCGG 2 cut(s) 18, 444
Hpy166II GTNNAC 1 cut(s) 133
Hpy188I TCNGA 4 cut(s) 217, 297, 369, 381
Hpy188III TCNNGA 5 cut(s) 40, 121, 233, 249, 439
Hpy8I GTNNAC 1 cut(s) 133
Hpy99I CGWCG 5 cut(s) 14, 134, 137, 140, 268
HpyAV CCTTC 2 cut(s) 277, 423
HpyCH4IV ACGT 2 cut(s) 129, 135
HpyCH4V TGCA 1 cut(s) 351
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 154, 308
HpyF3I CTNAG 3 cut(s) 79, 85, 303
HpySE526I ACGT 2 cut(s) 129, 135
Hsp92I GRCGYC 2 cut(s) 69, 135
Hsp92II CATG 1 cut(s) 166
HspAI GCGC 2 cut(s) 392, 422
Kzo9I GATC 3 cut(s) 96, 221, 381
LmnI GCTCC 1 cut(s) 42
LpnPI CCDG 9 cut(s) 31, 50, 53, 87, 219, 306, 317, 374, 417
Lsp1109I GCAGC 2 cut(s) 76, 132
MaeI CTAG 1 cut(s) 411
MaeII ACGT 2 cut(s) 129, 135
MalI GATC 3 cut(s) 98, 223, 383
MboI GATC 3 cut(s) 96, 221, 381
MboII GAAGA 2 cut(s) 55, 438
MhlI GDGCHC 3 cut(s) 39, 309, 331
MnlI CCTC 6 cut(s) 16, 103, 116, 187, 282, 411
MspA1I CMGCKG 1 cut(s) 89
MspI CCGG 2 cut(s) 18, 444
MvnI CGCG 2 cut(s) 311, 424
MwoI GCNNNNNNNGC 3 cut(s) 11, 154, 308
NdeII GATC 3 cut(s) 96, 221, 381
NlaIII CATG 1 cut(s) 166
NlaIV GGNNCC 2 cut(s) 241, 378
PceI AGGCCT 1 cut(s) 105
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 135
PfeI GAWTC 4 cut(s) 27, 140, 292, 370
PkrI GCNGC 3 cut(s) 91, 147, 313
Psp124BI GAGCTC 2 cut(s) 39, 309
PspN4I GGNNCC 2 cut(s) 241, 378
PspPI GGNCC 1 cut(s) 239
PvuII CAGCTG 1 cut(s) 89
RsaI GTAC 1 cut(s) 50
RsaNI GTAC 1 cut(s) 49
SacI GAGCTC 2 cut(s) 39, 309
SalI GTCGAC 1 cut(s) 131
SatI GCNGC 3 cut(s) 90, 146, 312
Sau3AI GATC 3 cut(s) 96, 221, 381
Sau96I GGNCC 1 cut(s) 239
SduI GDGCHC 3 cut(s) 39, 309, 331
SgrAI CRCCGGYG 1 cut(s) 17
SgrDI CGTCGACG 1 cut(s) 131
SinI GGWCC 1 cut(s) 239
SseBI AGGCCT 1 cut(s) 105
SsiI CCGC 3 cut(s) 21, 311, 447
SspMI CTAG 1 cut(s) 411
SstI GAGCTC 2 cut(s) 39, 309
StuI AGGCCT 1 cut(s) 105
TaiI ACGT 2 cut(s) 132, 138
TaqI TCGA 6 cut(s) 25, 95, 132, 138, 263, 317
TauI GCSGC 1 cut(s) 314
TfiI GAWTC 4 cut(s) 27, 140, 292, 370
TscAI CASTG 1 cut(s) 69
TseI GCWGC 2 cut(s) 89, 145
TspDTI ATGAA 1 cut(s) 424
TspGWI ACGGA 1 cut(s) 252
TspRI CASTG 1 cut(s) 69
VpaK11BI GGWCC 1 cut(s) 239
XcmI CCANNNNNNNNNTGG 1 cut(s) 160
XmiI GTMKAC 1 cut(s) 132
XspI CTAG 1 cut(s) 411
ZraI GACGTC 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.