FvH4_2g18410

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0006974
Physical Location & Seq
Forward (+)
15785450 .. 15787569
2120 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g18410.t2

Sequence Viewer

Length: 966 bp
ATGTTGAGCCAGTATCTGATGAAGTTTCTTTCTCATGTATTAGCCATTACTGGACTTCTAGTCTTGGTTTTCTTGTATCTATGGAGGCTGAGATTTCAGAATCACAAAATGAAGGGCCTCATGTCCCCTGAACCCTATGGTGCCCTGCCAATTTTAGGTCACCTCCATCAACTAGGTGGCCGAAACCCACTGTTTCAAACCTTAGCAAACATGGCTGACAAGTATGGTCCAATCTTTACCATATGGCTCGGCAAGAACCGTGCCCTGGTGATCAGCAACTACGAAGCAGCCAAGGAATGTTTCACCACAAACGACAGAGTTTTCGCCACAAGACCGAGTTCTGCACAGGGAAAGTACCTTGGTTACAACTATGCAGCATTTGGGGTCTCACCTTATGGAAAATATTGGCGTGACACGAGAAAGTTGGTCGTCATGGAGTTGCTCTCTAGTCGTAGGCTAGAGACTCTGAAACATGTACAAATCTCTGAGGTTGATGCTTTTGTTATAGGGTTGTATTCAGTTTGGAAAAACAAAGGACATGATGGTCCAAGCAAGGTGGTGATCAGTACGGGGATTGAGCACCTAACATGTAATGTGATGACTAAAATTATTGCTGGGAAGAGGTACTTTGGTGTAAAAAGTGATGAATTTGACGGGGAGGTAAAGAAGATAAGGAAGCTTATGAAGGAGTTTATGTACGCAGCCGTTAATCGGGTTGTTTCTTATGTAGTTGGGATTCCAGAGTGGATAGATTTCAAGGGGCATTTGAGAACTATGAAGAATGTAGGAGGCCAGTTAGACTCTTTGATGACAAGTTGGGTTGAAGAACATAATACAAAGAAGCGTAAGAATGATTCCAGCAACGAGGAGCAGGACTTCATTGATGTCCTGCTGTCTGAGATTGAGGACGGTTCTATGCTTGGTCATACCCGAGATACTATTATCAAATCAACAGCACTGGTGTAA

Protein Analysis

322

Amino Acids

36.54

Weight (kDa)

9.43

Isoelectric Point (pI)

32.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 44 - 304 1.7e-28 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 543
AccB1I GGYRCC 1 cut(s) 140
AcoI YGGCCR 1 cut(s) 178
AcsI RAATTY 1 cut(s) 647
AfaI GTAC 5 cut(s) 356, 477, 568, 626, 698
AfiI CCNNNNNNNGG 3 cut(s) 155, 265, 711
AflIII ACRYGT 2 cut(s) 472, 587
AgsI TTSAA 3 cut(s) 197, 757, 824
AhdI GACNNNNNGTC 1 cut(s) 59
AjnI CCWGG 1 cut(s) 264
AjuI GAANNNNNNNTTGG 2 cut(s) 284, 316
AluBI AGCT 1 cut(s) 679
AluI AGCT 1 cut(s) 679
Alw21I GWGCWC 1 cut(s) 582
Alw26I GTCTC 2 cut(s) 391, 455
AlwNI CAGNNNCTG 1 cut(s) 16
Ama87I CYCGRG 1 cut(s) 930
AoxI GGCC 3 cut(s) 115, 178, 790
ApeKI GCWGC 3 cut(s) 287, 374, 701
ApoI RAATTY 1 cut(s) 647
ArsI GACNNNNNNTTYG 2 cut(s) 305, 337
AspS9I GGNCC 3 cut(s) 115, 227, 545
AsuHPI GGTGA 5 cut(s) 152, 280, 295, 381, 571
AvaI CYCGRG 1 cut(s) 930
AvaII GGWCC 2 cut(s) 227, 545
BaeGI GKGCMC 2 cut(s) 145, 265
BaeI ACNNNNGTAYC 2 cut(s) 616, 649
BanI GGYRCC 1 cut(s) 140
BauI CACGAG 1 cut(s) 415
Bbv12I GWGCWC 1 cut(s) 582
BbvI GCAGC 3 cut(s) 299, 386, 713
BccI CCATC 2 cut(s) 174, 536
BceAI ACGGC 1 cut(s) 689
BciT130I CCWGG 1 cut(s) 266
BclI TGATCA 2 cut(s) 270, 561
BcoDI GTCTC 2 cut(s) 391, 455
BfaI CTAG 4 cut(s) 59, 173, 447, 458
BisI GCNGC 3 cut(s) 288, 375, 702
BlsI GCNGC 3 cut(s) 289, 376, 703
Bme1390I CCNGG 1 cut(s) 266
Bme18I GGWCC 2 cut(s) 227, 545
BmeRI GACNNNNNGTC 1 cut(s) 59
BmeT110I CYCGRG 1 cut(s) 930
BmgT120I GGNCC 3 cut(s) 115, 227, 545
BmiI GGNNCC 1 cut(s) 142
BmrFI CCNGG 1 cut(s) 266
BmsI GCATC 1 cut(s) 484
BplI GAGNNNNNCTC 2 cut(s) 428, 460
Bpu10I CCTNAGC 1 cut(s) 202
BsaI GGTCTC 1 cut(s) 391
BsaJI CCNNGG 3 cut(s) 264, 291, 358
BsaXI ACNNNNNCTCC 2 cut(s) 680, 710
Bsc4I CCNNNNNNNGG 3 cut(s) 155, 265, 711
Bse1I ACTGG 4 cut(s) 10, 55, 793, 963
BseBI CCWGG 1 cut(s) 266
BseDI CCNNGG 3 cut(s) 264, 291, 358
BseLI CCNNNNNNNGG 3 cut(s) 155, 265, 711
BseMII CTCAG 3 cut(s) 80, 477, 888
BseNI ACTGG 4 cut(s) 10, 55, 793, 963
BseRI GAGGAG 1 cut(s) 881
BseSI GKGCMC 2 cut(s) 145, 265
BseXI GCAGC 3 cut(s) 299, 386, 713
BseYI CCCAGC 1 cut(s) 614
BsgI GTGCAG 1 cut(s) 327
BshFI GGCC 3 cut(s) 117, 180, 792
BshNI GGYRCC 1 cut(s) 140
BsiHKAI GWGCWC 1 cut(s) 582
BsiHKCI CYCGRG 1 cut(s) 930
BslFI GGGAC 1 cut(s) 109
BslI CCNNNNNNNGG 3 cut(s) 155, 265, 711
BsmAI GTCTC 2 cut(s) 391, 455
BsmFI GGGAC 1 cut(s) 109
BsnI GGCC 3 cut(s) 117, 180, 792
Bso31I GGTCTC 1 cut(s) 391
BsoBI CYCGRG 1 cut(s) 930
Bsp1286I GDGCHC 3 cut(s) 145, 265, 582
Bsp1407I TGTACA 1 cut(s) 475
Bsp143I GATC 2 cut(s) 270, 561
BspANI GGCC 3 cut(s) 117, 180, 792
BspCNI CTCAG 3 cut(s) 81, 478, 889
BspLI GGNNCC 1 cut(s) 142
BspT107I GGYRCC 1 cut(s) 140
BspTNI GGTCTC 1 cut(s) 391
BsrGI TGTACA 1 cut(s) 475
BsrI ACTGG 4 cut(s) 10, 55, 793, 963
BssECI CCNNGG 3 cut(s) 264, 291, 358
BssMI GATC 2 cut(s) 270, 561
BssSI CACGAG 1 cut(s) 415
BssT1I CCWWGG 2 cut(s) 291, 358
Bst2BI CACGAG 1 cut(s) 415
Bst2UI CCWGG 1 cut(s) 266
Bst4CI ACNGT 3 cut(s) 192, 260, 911
Bst6I CTCTTC 1 cut(s) 614
BstAUI TGTACA 1 cut(s) 475
BstDEI CTNAG 4 cut(s) 89, 202, 486, 897
BstEII GGTNACC 1 cut(s) 158
BstKTI GATC 2 cut(s) 273, 564
BstMAI GTCTC 2 cut(s) 391, 455
BstMBI GATC 2 cut(s) 270, 561
BstMWI GCNNNNNNNGC 1 cut(s) 212
BstNI CCWGG 1 cut(s) 266
BstNSI RCATGY 2 cut(s) 476, 591
BstPI GGTNACC 1 cut(s) 158
BstSCI CCNGG 1 cut(s) 264
BstSLI GKGCMC 2 cut(s) 145, 265
BstV1I GCAGC 3 cut(s) 299, 386, 713
BsuRI GGCC 3 cut(s) 117, 180, 792
BtsIMutI CAGTG 2 cut(s) 188, 956
CaiI CAGNNNCTG 1 cut(s) 16
Cfr13I GGNCC 3 cut(s) 115, 227, 545
Csp6I GTAC 5 cut(s) 355, 476, 567, 625, 697
CspCI CAANNNNNGTGG 2 cut(s) 537, 572
CviAII CATG 7 cut(s) 35, 121, 211, 433, 473, 539, 588
CviQI GTAC 5 cut(s) 355, 476, 567, 625, 697
DdeI CTNAG 4 cut(s) 89, 202, 486, 897
DpnI GATC 2 cut(s) 272, 563
DpnII GATC 2 cut(s) 270, 561
DrdI GACNNNNNNGTC 1 cut(s) 543
DriI GACNNNNNGTC 1 cut(s) 59
DseDI GACNNNNNNGTC 1 cut(s) 543
EaeI YGGCCR 1 cut(s) 178
Eam1104I CTCTTC 1 cut(s) 614
Eam1105I GACNNNNNGTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 614
Eco130I CCWWGG 2 cut(s) 291, 358
Eco31I GGTCTC 1 cut(s) 391
Eco47I GGWCC 2 cut(s) 227, 545
Eco88I CYCGRG 1 cut(s) 930
Eco91I GGTNACC 1 cut(s) 158
EcoO109I RGGNCCY 1 cut(s) 115
EcoO65I GGTNACC 1 cut(s) 158
EcoRII CCWGG 1 cut(s) 264
EcoT14I CCWWGG 2 cut(s) 291, 358
ErhI CCWWGG 2 cut(s) 291, 358
FaeI CATG 7 cut(s) 38, 124, 214, 436, 476, 542, 591
FalI AAGNNNNNCTT 2 cut(s) 611, 643
FaqI GGGAC 1 cut(s) 109
FatI CATG 7 cut(s) 34, 120, 210, 432, 472, 538, 587
FauNDI CATATG 1 cut(s) 242
FbaI TGATCA 2 cut(s) 270, 561
Fnu4HI GCNGC 3 cut(s) 288, 375, 702
Fsp4HI GCNGC 3 cut(s) 288, 375, 702
FspBI CTAG 4 cut(s) 59, 173, 447, 458
GluI GCNGC 3 cut(s) 288, 375, 702
GsaI CCCAGC 1 cut(s) 618
HaeIII GGCC 3 cut(s) 117, 180, 792
Hin1II CATG 7 cut(s) 38, 124, 214, 436, 476, 542, 591
HindIII AAGCTT 1 cut(s) 677
HinfI GANTC 5 cut(s) 100, 463, 736, 800, 854
HphI GGTGA 5 cut(s) 152, 280, 295, 381, 571
Hpy188I TCNGA 5 cut(s) 18, 99, 468, 487, 898
Hpy188III TCNNGA 1 cut(s) 740
HpyAV CCTTC 2 cut(s) 106, 679
HpyCH4III ACNGT 3 cut(s) 192, 260, 911
HpyCH4V TGCA 2 cut(s) 344, 374
HpyF10VI GCNNNNNNNGC 1 cut(s) 212
HpyF3I CTNAG 4 cut(s) 89, 202, 486, 897
Hsp92II CATG 7 cut(s) 38, 124, 214, 436, 476, 542, 591
Ksp22I TGATCA 2 cut(s) 270, 561
Kzo9I GATC 2 cut(s) 270, 561
LmnI GCTCC 1 cut(s) 868
Lsp1109I GCAGC 3 cut(s) 299, 386, 713
LweI GCATC 1 cut(s) 484
MaeI CTAG 4 cut(s) 59, 173, 447, 458
MaeIII GTNAC 3 cut(s) 158, 362, 410
MalI GATC 2 cut(s) 272, 563
MboI GATC 2 cut(s) 270, 561
MboII GAAGA 4 cut(s) 631, 679, 790, 836
MhlI GDGCHC 3 cut(s) 145, 265, 582
MluCI AATT 3 cut(s) 150, 606, 647
MlyI GAGTC 2 cut(s) 457, 794
MnlI CCTC 9 cut(s) 78, 128, 173, 481, 615, 652, 782, 859, 898
MseI TTAA 1 cut(s) 708
MspR9I CCNGG 1 cut(s) 266
MvaI CCWGG 1 cut(s) 266
MwoI GCNNNNNNNGC 1 cut(s) 212
NdeI CATATG 1 cut(s) 242
NdeII GATC 2 cut(s) 270, 561
NlaIII CATG 7 cut(s) 38, 124, 214, 436, 476, 542, 591
NlaIV GGNNCC 1 cut(s) 142
NmeAIII GCCGAG 1 cut(s) 228
NmuCI GTSAC 2 cut(s) 158, 410
NspI RCATGY 2 cut(s) 476, 591
PciI ACATGT 2 cut(s) 472, 587
PfeI GAWTC 3 cut(s) 100, 736, 854
PkrI GCNGC 3 cut(s) 289, 376, 703
PleI GAGTC 2 cut(s) 457, 794
PpsI GAGTC 2 cut(s) 457, 794
PscI ACATGT 2 cut(s) 472, 587
Psp6I CCWGG 1 cut(s) 264
PspEI GGTNACC 1 cut(s) 158
PspFI CCCAGC 1 cut(s) 614
PspGI CCWGG 1 cut(s) 264
PspN4I GGNNCC 1 cut(s) 142
PspPI GGNCC 3 cut(s) 115, 227, 545
PstNI CAGNNNCTG 1 cut(s) 16
RsaI GTAC 5 cut(s) 356, 477, 568, 626, 698
RsaNI GTAC 5 cut(s) 355, 476, 567, 625, 697
SaqAI TTAA 1 cut(s) 708
SatI GCNGC 3 cut(s) 288, 375, 702
Sau3AI GATC 2 cut(s) 270, 561
Sau96I GGNCC 3 cut(s) 115, 227, 545
SchI GAGTC 2 cut(s) 457, 794
ScrFI CCNGG 1 cut(s) 266
SduI GDGCHC 3 cut(s) 145, 265, 582
SfaNI GCATC 1 cut(s) 484
SinI GGWCC 2 cut(s) 227, 545
Sse9I AATT 3 cut(s) 150, 606, 647
SspI AATATT 1 cut(s) 404
SspMI CTAG 4 cut(s) 59, 173, 447, 458
StyD4I CCNGG 1 cut(s) 264
StyI CCWWGG 2 cut(s) 291, 358
TaaI ACNGT 3 cut(s) 192, 260, 911
TaqII GACCGA 1 cut(s) 349
TasI AATT 3 cut(s) 150, 606, 647
TatI WGTACW 1 cut(s) 475
TfiI GAWTC 3 cut(s) 100, 736, 854
Tru1I TTAA 1 cut(s) 708
Tru9I TTAA 1 cut(s) 708
TscAI CASTG 2 cut(s) 195, 963
TseFI GTSAC 2 cut(s) 158, 410
TseI GCWGC 3 cut(s) 287, 374, 701
Tsp45I GTSAC 2 cut(s) 158, 410
TspDTI ATGAA 6 cut(s) 35, 125, 660, 698, 791, 868
TspRI CASTG 2 cut(s) 195, 963
VpaK11BI GGWCC 2 cut(s) 227, 545
XapI RAATTY 1 cut(s) 647
XceI RCATGY 2 cut(s) 476, 591
XcmI CCANNNNNNNNNTGG 1 cut(s) 173
XspI CTAG 4 cut(s) 59, 173, 447, 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.