Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0006974

Physical Location & Seq

Genomic Coordinates Forward (+)

29970179 .. 29972569

Gene Structure 2391 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1170000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1575 bp

ATGGATTTTCCTTCTCATCTTCTAGCAATTGTAGGGATTTTACTCTTTGTTCTGTATCTATGGAGGGTAAAAATCCAAAGTCACAAAACCAAGGGCATGCTAGCCCCACAACCATCAGGTGCCCTTCCAATCATAGGACACCTCCACAAACTTGGAGGCAAAAACCCACTTTGCAGAACCCTAGCATCCATGGCTGACAAGTACGGTCCAATCTTCACCATACAGCTTGGCAAGCACCCTGCACTTGTGATCAGCAACTATGATGCAGTCAAGGAATGCTTCACCAAAAACGACATTGCTTTCGCAACGAGATCAAAGTCGGCACAAGGAAGGTACCTTGGTTACAATTATGCAGCTTTAGGGTTCTCACAATACGGCACGTATTGGCGTAACATGAGAAAAATGGCCATCGTTGAGTTGCTCTCTAGCCGCAGGCTTCAGACACTAAAACATGTCCAAATCTCTGAGGTTGATTCTTTTATCAAAGGGTTGTACGAAATTTGCAAAACAAAAGGGGAAAATGGTCAAAGCAAGGTGGTGATTAGCGAGTGGATTGAGCACCTGACTTTGAATGTAATTACTCAAATGATTGCTGGGAAGAGATATTTTGATTGGGGTAAAGTGGGAAATGACTCAGATAATGAGGGAGAAAATAAGAGGATTCGGGAAATGGTGAAGGAGTTTATGTGTGTGGCTGGGAAACCAGTTGTTTCTGATGTAATTGGGTTTCCGGATTGGATTGATCTAATGGGGCAAGTGAAGAATATGAAGCGTATCGGGAAGGATTTTGATTCTCTAATGGCAAGTTGGATTGAAGAACATAAGAATGCAAGGGAGGAAATTGACTCAAGGGACAAGCTTGACTTCATTGATGTCATGCTTTCTGTGATTGAGGACAATACTGTGCTTGGTCATAGTCGTGAAACTATCATCAAGGCAACATCGCAGAATCTGATCTTAGCAGGCTCAGACACCACAGCGACCAGCTTGACGTGGATCCTCTCTTTATTATTAAACAATAGGCATATTTTGAAGCATGCACAAGAAGAGATAAACCTACAAGTTGGCACAGAGAGATGGGTTGAAGACACTGATATCAAAGACCTAACTTACCTCCAAGCCATTGTCAAGGAAACCTTACGTCTATACCCTCCAGCCCCACTCTCAGTCCCACATGAAGCAATGGAAGATTGCCAAGTTTGTGGCTTTCACATTCCAAAGGGCACACGTTTATTTGCGAATTTGTGGAAATTGCATAGAGACCCATCTGTGTGGTCAGACCCTGAAGTGTTTTGCCCAGATAGGTTTCTTACAACCCAAGCAAGCACTGATGTCTCTGGTCAACATTTTGAGTTCATTCCATTTGGATCTGGAAGAAGATCTTGTCCAGGTCTCACATTCGCAATGCAAGTTATCCACTTGACTCTTGGTAGATTGATTCAAGGGTTTGAATTGGCCACTCCATTGGACATGCCTGTGGACATGGCTGAAGGACTAGGTATTACTATGCCAAAGGCAACTTCACTTGAAGTCGTGCTAACTCCACGCTTGCCCAGTCAATTCTATGAAAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0008150 GO:0008152 GO:0009058 GO:0009987 GO:0010035 GO:0010038 GO:0010039 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0042221 GO:0044550 GO:0050896 GO:0051716 GO:0055114 GO:0070887 GO:0071241 GO:0071248 GO:0071281

KEGG Pathways

Metabolic & Signaling

ko00140 ko01100 ko04913 ko04917 ko04927 ko04934 map00140 map01100 map04913 map04917 map04927 map04934

Pfam Domains

Protein Families

p450

Protein Analysis

525

Amino Acids

59.13

Weight (kDa)

7.26

Isoelectric Point (pI)

41.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
p450	PF00067	36 - 505	3.9e-98	Cytochrome P450

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006974)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g18410 FvH4_2g18410 FvH4_2g18411 FvH4_2g18420
malus_domestica	MD05G1169700.v1.1 MD05G1169800.v1.1 MD05G1169900.v1.1 MD05G1170000.v1.1 MD05G1170100.v1.1
prunus_persica	Prupe.8G197100_v2.0.a1 Prupe.8G197300_v2.0.a1
pyrus_communis	pycom05g15720 pycom05g15730
rosa_chinensis	RchiOBHm_Chr6g0283841 RchiOBHm_Chr6g0283881
rosa_laevigata	RLG00000012789 RLG00000012792
rosa_multiflora	Rmu_ssc0000442.1_g000004
rosa_roxburghii	Rroxscaffold_7G00185130 Rroxscaffold_7G00185140
rosa_rugosa	Rorug06G0158100
rosa_samantha	Rh6CG273300
rosa_wichuraiana	Rw6G023320 Rw6G023330 Rw6G023380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	333
AccB1I	GGYRCC	2 cut(s)	119, 333
AccIII	TCCGGA	1 cut(s)	730
AciI	CCGC	1 cut(s)	430
AclWI	GGATC	3 cut(s)	991, 1004, 1375
AcoI	YGGCCR	2 cut(s)	405, 1455
AcsI	RAATTY	2 cut(s)	498, 1240
AcuI	CTGAAG	3 cut(s)	422, 1305, 1509
AfaI	GTAC	3 cut(s)	203, 335, 494
AfiI	CCNNNNNNNGG	1 cut(s)	134
AflIII	ACRYGT	2 cut(s)	451, 1226
AgsI	TTSAA	7 cut(s)	571, 815, 1033, 1085, 1442, 1451, 1529
AjiI	CACGTC	1 cut(s)	993
AjnI	CCWGG	1 cut(s)	1387
AjuI	GAANNNNNNNTTGG	2 cut(s)	1504, 1536
AluBI	AGCT	4 cut(s)	226, 356, 859, 987
AluI	AGCT	4 cut(s)	226, 356, 859, 987
Alw21I	GWGCWC	1 cut(s)	561
Alw26I	GTCTC	3 cut(s)	1254, 1339, 1397
AlwI	GGATC	3 cut(s)	991, 1004, 1375
AlwNI	CAGNNNCTG	2 cut(s)	952, 1283
Aor13HI	TCCGGA	1 cut(s)	730
AoxI	GGCC	2 cut(s)	405, 1455
ApeKI	GCWGC	1 cut(s)	353
ApoI	RAATTY	2 cut(s)	498, 1240
ArsI	GACNNNNNNTTYG	2 cut(s)	284, 316
Asp718I	GGTACC	1 cut(s)	333
AspS9I	GGNCC	1 cut(s)	206
AsuHPI	GGTGA	4 cut(s)	208, 274, 550, 685
AsuNHI	GCTAGC	1 cut(s)	100
AvaII	GGWCC	1 cut(s)	206
BaeGI	GKGCMC	2 cut(s)	124, 1226
BaeI	ACNNNNGTAYC	2 cut(s)	325, 358
BalI	TGGCCA	2 cut(s)	407, 1457
BamHI	GGATCC	1 cut(s)	996
BanI	GGYRCC	2 cut(s)	119, 333
BbsI	GAAGAC	1 cut(s)	1092
Bbv12I	GWGCWC	1 cut(s)	561
BbvI	GCAGC	1 cut(s)	365
BccI	CCATC	4 cut(s)	121, 416, 1071, 1273
BceAI	ACGGC	1 cut(s)	391
BciT130I	CCWGG	1 cut(s)	1389
BclI	TGATCA	1 cut(s)	249
BcoDI	GTCTC	3 cut(s)	1254, 1339, 1397
BfaI	CTAG	5 cut(s)	23, 101, 182, 426, 1496
BglII	AGATCT	1 cut(s)	1379
BisI	GCNGC	2 cut(s)	354, 430
BlsI	GCNGC	2 cut(s)	355, 431
Bme1390I	CCNGG	1 cut(s)	1389
Bme18I	GGWCC	1 cut(s)	206
BmgBI	CACGTC	1 cut(s)	993
BmgT120I	GGNCC	1 cut(s)	206
BmiI	GGNNCC	3 cut(s)	121, 335, 998
BmrFI	CCNGG	1 cut(s)	1389
BmrI	ACTGGG	1 cut(s)	1548
BmsI	GCATC	2 cut(s)	194, 253
BmtI	GCTAGC	1 cut(s)	104
BmuI	ACTGGG	1 cut(s)	1548
BpiI	GAAGAC	1 cut(s)	1092
BplI	GAGNNNNNCTC	2 cut(s)	407, 439
BpmI	CTGGAG	1 cut(s)	1137
BpuEI	CTTGAG	1 cut(s)	832
BsaAI	YACGTR	1 cut(s)	381
BsaI	GGTCTC	2 cut(s)	1254, 1397
BsaJI	CCNNGG	3 cut(s)	90, 189, 337
BsaWI	WCCGGW	1 cut(s)	730
BsaXI	ACNNNNNCTCC	2 cut(s)	671, 701
Bsc4I	CCNNNNNNNGG	1 cut(s)	134
Bse1I	ACTGG	2 cut(s)	704, 1554
Bse3DI	GCAATG	3 cut(s)	294, 1188, 1410
BseAI	TCCGGA	1 cut(s)	730
BseBI	CCWGG	1 cut(s)	1389
BseDI	CCNNGG	3 cut(s)	90, 189, 337
BseGI	GGATG	1 cut(s)	185
BseLI	CCNNNNNNNGG	1 cut(s)	134
BseMI	GCAATG	3 cut(s)	294, 1188, 1410
BseMII	CTCAG	4 cut(s)	456, 648, 981, 1179
BseNI	ACTGG	2 cut(s)	704, 1554
BseSI	GKGCMC	2 cut(s)	124, 1226
BseXI	GCAGC	1 cut(s)	365
BseYI	CCCAGC	2 cut(s)	593, 695
BsgI	GTGCAG	1 cut(s)	225
BshFI	GGCC	2 cut(s)	407, 1457
BshNI	GGYRCC	2 cut(s)	119, 333
BsiHKAI	GWGCWC	1 cut(s)	561
BsiSI	CCGG	1 cut(s)	731
BslFI	GGGAC	2 cut(s)	866, 1154
BslI	CCNNNNNNNGG	1 cut(s)	134
BsmAI	GTCTC	3 cut(s)	1254, 1339, 1397
BsmFI	GGGAC	2 cut(s)	866, 1154
BsmI	GAATGC	2 cut(s)	281, 832
BsnI	GGCC	2 cut(s)	407, 1457
Bso31I	GGTCTC	2 cut(s)	1254, 1397
Bsp1286I	GDGCHC	3 cut(s)	124, 561, 1226
Bsp13I	TCCGGA	1 cut(s)	730
Bsp143I	GATC	7 cut(s)	249, 311, 742, 954, 996, 1367, 1379
Bsp19I	CCATGG	1 cut(s)	189
BspACI	CCGC	1 cut(s)	430
BspANI	GGCC	2 cut(s)	407, 1457
BspCNI	CTCAG	4 cut(s)	457, 647, 980, 1178
BspEI	TCCGGA	1 cut(s)	730
BspLI	GGNNCC	3 cut(s)	121, 335, 998
BspOI	GCTAGC	1 cut(s)	104
BspPI	GGATC	3 cut(s)	991, 1004, 1375
BspT107I	GGYRCC	2 cut(s)	119, 333
BspTNI	GGTCTC	2 cut(s)	1254, 1397
BsrDI	GCAATG	3 cut(s)	294, 1188, 1410
BsrI	ACTGG	2 cut(s)	704, 1554
BssECI	CCNNGG	3 cut(s)	90, 189, 337
BssMI	GATC	7 cut(s)	249, 311, 742, 954, 996, 1367, 1379
BssT1I	CCWWGG	3 cut(s)	90, 189, 337
Bst2UI	CCWGG	1 cut(s)	1389
Bst4CI	ACNGT	2 cut(s)	206, 904
Bst6I	CTCTTC	2 cut(s)	593, 1041
BstBAI	YACGTR	1 cut(s)	381
BstC8I	GCNNGC	8 cut(s)	98, 102, 233, 434, 964, 1038, 1324, 1550
BstDEI	CTNAG	5 cut(s)	465, 634, 958, 967, 1165
BstDSI	CCRYGG	1 cut(s)	189
BstF5I	GGATG	1 cut(s)	185
BstKTI	GATC	7 cut(s)	252, 314, 745, 957, 999, 1370, 1382
BstMAI	GTCTC	3 cut(s)	1254, 1339, 1397
BstMBI	GATC	7 cut(s)	249, 311, 742, 954, 996, 1367, 1379
BstMWI	GCNNNNNNNGC	2 cut(s)	191, 232
BstNI	CCWGG	1 cut(s)	1389
BstNSI	RCATGY	4 cut(s)	100, 455, 1040, 1474
BstSCI	CCNGG	1 cut(s)	1387
BstSLI	GKGCMC	2 cut(s)	124, 1226
BstV1I	GCAGC	1 cut(s)	365
BstV2I	GAAGAC	1 cut(s)	1092
BstX2I	RGATCY	3 cut(s)	996, 1367, 1379
BstXI	CCANNNNNNTGG	4 cut(s)	152, 1202, 1272, 1465
BstYI	RGATCY	3 cut(s)	996, 1367, 1379
BsuRI	GGCC	2 cut(s)	407, 1457
BtgI	CCRYGG	1 cut(s)	189
BtgZI	GCGATG	1 cut(s)	927
BtrI	CACGTC	1 cut(s)	993
BtsCI	GGATG	1 cut(s)	185
BtsIMutI	CAGTG	2 cut(s)	1089, 1326
Cac8I	GCNNGC	8 cut(s)	98, 102, 233, 434, 964, 1038, 1324, 1550
CaiI	CAGNNNCTG	2 cut(s)	952, 1283
Cfr13I	GGNCC	1 cut(s)	206
Csp6I	GTAC	3 cut(s)	202, 334, 493
CviAII	CATG	9 cut(s)	97, 190, 394, 452, 877, 1037, 1175, 1471, 1483
CviQI	GTAC	3 cut(s)	202, 334, 493
DdeI	CTNAG	5 cut(s)	465, 634, 958, 967, 1165
DpnI	GATC	7 cut(s)	251, 313, 744, 956, 998, 1369, 1381
DpnII	GATC	7 cut(s)	249, 311, 742, 954, 996, 1367, 1379
EaeI	YGGCCR	2 cut(s)	405, 1455
Eam1104I	CTCTTC	2 cut(s)	593, 1041
EarI	CTCTTC	2 cut(s)	593, 1041
Eco130I	CCWWGG	3 cut(s)	90, 189, 337
Eco31I	GGTCTC	2 cut(s)	1254, 1397
Eco32I	GATATC	1 cut(s)	1096
Eco47I	GGWCC	1 cut(s)	206
Eco57I	CTGAAG	3 cut(s)	422, 1305, 1509
EcoRII	CCWGG	1 cut(s)	1387
EcoRV	GATATC	1 cut(s)	1096
EcoT14I	CCWWGG	3 cut(s)	90, 189, 337
ErhI	CCWWGG	3 cut(s)	90, 189, 337
FaeI	CATG	9 cut(s)	100, 193, 397, 455, 880, 1040, 1178, 1474, 1486
FalI	AAGNNNNNCTT	8 cut(s)	263, 295, 848, 880, 1121, 1153, 1366, 1398
FaqI	GGGAC	2 cut(s)	866, 1154
FatI	CATG	9 cut(s)	96, 189, 393, 451, 876, 1036, 1174, 1470, 1482
FbaI	TGATCA	1 cut(s)	249
Fnu4HI	GCNGC	2 cut(s)	354, 430
FokI	GGATG	1 cut(s)	172
Fsp4HI	GCNGC	2 cut(s)	354, 430
FspBI	CTAG	5 cut(s)	23, 101, 182, 426, 1496
GluI	GCNGC	2 cut(s)	354, 430
GsaI	CCCAGC	2 cut(s)	597, 699
GsuI	CTGGAG	1 cut(s)	1137
HaeIII	GGCC	2 cut(s)	407, 1457
HapII	CCGG	1 cut(s)	731
Hin1II	CATG	9 cut(s)	100, 193, 397, 455, 880, 1040, 1178, 1474, 1486
HincII	GTYRAC	1 cut(s)	1343
HindII	GTYRAC	1 cut(s)	1343
HindIII	AAGCTT	1 cut(s)	857
HinfI	GANTC	8 cut(s)	473, 632, 661, 791, 845, 949, 1423, 1438
HpaII	CCGG	1 cut(s)	731
HphI	GGTGA	4 cut(s)	208, 274, 550, 685
Hpy166II	GTNNAC	2 cut(s)	1343, 1480
Hpy188I	TCNGA	7 cut(s)	441, 466, 637, 715, 954, 970, 1279
Hpy188III	TCNNGA	5 cut(s)	665, 731, 778, 920, 1371
Hpy8I	GTNNAC	2 cut(s)	1343, 1480
HpyAV	CCTTC	6 cut(s)	21, 134, 324, 670, 775, 1484
HpyCH4III	ACNGT	2 cut(s)	206, 904
HpyCH4IV	ACGT	4 cut(s)	380, 992, 1141, 1228
HpyCH4V	TGCA	9 cut(s)	174, 242, 266, 353, 504, 830, 1040, 1255, 1408
HpyF10VI	GCNNNNNNNGC	2 cut(s)	191, 232
HpyF3I	CTNAG	5 cut(s)	465, 634, 958, 967, 1165
HpySE526I	ACGT	4 cut(s)	380, 992, 1141, 1228
Hsp92II	CATG	9 cut(s)	100, 193, 397, 455, 880, 1040, 1178, 1474, 1486
Kpn2I	TCCGGA	1 cut(s)	730
KpnI	GGTACC	1 cut(s)	337
Ksp22I	TGATCA	1 cut(s)	249
Kzo9I	GATC	7 cut(s)	249, 311, 742, 954, 996, 1367, 1379
Lsp1109I	GCAGC	1 cut(s)	365
LweI	GCATC	2 cut(s)	194, 253
MaeI	CTAG	5 cut(s)	23, 101, 182, 426, 1496
MaeII	ACGT	4 cut(s)	380, 992, 1141, 1228
MaeIII	GTNAC	3 cut(s)	80, 341, 389
MalI	GATC	7 cut(s)	251, 313, 744, 956, 998, 1369, 1381
MboI	GATC	7 cut(s)	249, 311, 742, 954, 996, 1367, 1379
MfeI	CAATTG	1 cut(s)	27
MflI	RGATCY	3 cut(s)	996, 1367, 1379
MhlI	GDGCHC	3 cut(s)	124, 561, 1226
MlsI	TGGCCA	2 cut(s)	407, 1457
MluNI	TGGCCA	2 cut(s)	407, 1457
MlyI	GAGTC	3 cut(s)	626, 839, 1417
MmeI	TCCRAC	1 cut(s)	788
Mox20I	TGGCCA	2 cut(s)	407, 1457
MroI	TCCGGA	1 cut(s)	730
MscI	TGGCCA	2 cut(s)	407, 1457
MseI	TTAA	1 cut(s)	1013
MslI	CAYNNNNRTG	4 cut(s)	825, 918, 1270, 1475
Msp20I	TGGCCA	2 cut(s)	407, 1457
MspI	CCGG	1 cut(s)	731
MspR9I	CCNGG	1 cut(s)	1389
MunI	CAATTG	1 cut(s)	27
Mva1269I	GAATGC	2 cut(s)	281, 832
MvaI	CCWGG	1 cut(s)	1389
MwoI	GCNNNNNNNGC	2 cut(s)	191, 232
NcoI	CCATGG	1 cut(s)	189
NdeII	GATC	7 cut(s)	249, 311, 742, 954, 996, 1367, 1379
NheI	GCTAGC	1 cut(s)	100
NlaIII	CATG	9 cut(s)	100, 193, 397, 455, 880, 1040, 1178, 1474, 1486
NlaIV	GGNNCC	3 cut(s)	121, 335, 998
NmuCI	GTSAC	1 cut(s)	80
NspI	RCATGY	4 cut(s)	100, 455, 1040, 1474
PaeI	GCATGC	2 cut(s)	100, 1040
PciI	ACATGT	1 cut(s)	451
PctI	GAATGC	2 cut(s)	281, 832
PfeI	GAWTC	5 cut(s)	473, 661, 791, 949, 1438
PkrI	GCNGC	2 cut(s)	355, 431
PleI	GAGTC	3 cut(s)	626, 839, 1417
PpsI	GAGTC	3 cut(s)	626, 839, 1417
Ppu21I	YACGTR	1 cut(s)	381
PscI	ACATGT	1 cut(s)	451
Psp6I	CCWGG	1 cut(s)	1387
PspFI	CCCAGC	2 cut(s)	593, 695
PspGI	CCWGG	1 cut(s)	1387
PspN4I	GGNNCC	3 cut(s)	121, 335, 998
PspPI	GGNCC	1 cut(s)	206
PstNI	CAGNNNCTG	2 cut(s)	952, 1283
PsuI	RGATCY	3 cut(s)	996, 1367, 1379
RsaI	GTAC	3 cut(s)	203, 335, 494
RsaNI	GTAC	3 cut(s)	202, 334, 493
RseI	CAYNNNNRTG	4 cut(s)	825, 918, 1270, 1475
SaqAI	TTAA	1 cut(s)	1013
SatI	GCNGC	2 cut(s)	354, 430
Sau3AI	GATC	7 cut(s)	249, 311, 742, 954, 996, 1367, 1379
Sau96I	GGNCC	1 cut(s)	206
SchI	GAGTC	3 cut(s)	626, 839, 1417
ScrFI	CCNGG	1 cut(s)	1389
SduI	GDGCHC	3 cut(s)	124, 561, 1226
SfaNI	GCATC	2 cut(s)	194, 253
SinI	GGWCC	1 cut(s)	206
SmiMI	CAYNNNNRTG	4 cut(s)	825, 918, 1270, 1475
SmlI	CTYRAG	1 cut(s)	847
SmoI	CTYRAG	1 cut(s)	847
SphI	GCATGC	2 cut(s)	100, 1040
SsiI	CCGC	1 cut(s)	430
SspMI	CTAG	5 cut(s)	23, 101, 182, 426, 1496
StyD4I	CCNGG	1 cut(s)	1387
StyI	CCWWGG	3 cut(s)	90, 189, 337
TaaI	ACNGT	2 cut(s)	206, 904
TaiI	ACGT	4 cut(s)	383, 995, 1144, 1231
TauI	GCSGC	1 cut(s)	432
TfiI	GAWTC	5 cut(s)	473, 661, 791, 949, 1438
Tru1I	TTAA	1 cut(s)	1013
Tru9I	TTAA	1 cut(s)	1013
TscAI	CASTG	2 cut(s)	1096, 1333
TseFI	GTSAC	1 cut(s)	80
TseI	GCWGC	1 cut(s)	353
Tsp45I	GTSAC	1 cut(s)	80
TspDTI	ATGAA	4 cut(s)	782, 856, 1191, 1345
TspRI	CASTG	2 cut(s)	1096, 1333
VpaK11BI	GGWCC	1 cut(s)	206
XapI	RAATTY	2 cut(s)	498, 1240
XceI	RCATGY	4 cut(s)	100, 455, 1040, 1474
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1424
XspI	CTAG	5 cut(s)	23, 101, 182, 426, 1496

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.