Rh6CG273300

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0006974
Physical Location & Seq
Forward (+)
44894911 .. 44897092
2182 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG273300.1

Sequence Viewer

Length: 1158 bp
ATGGATTCTCTTTCTCATCTACTAGCAATTACAGGAATTCTAGTTTTGGTTTTGTTGTATCTATGGAGGGTGAGATTTCAGAATCACAAAATGAAGGGCCTTATGTGCCCAGAACCCTATGGTGCCCTGCCAATTATAGGTCACCTCCATCAACTAGGTGGCCGAAACCCAGTGTTCCGAACCTTATCAACCATGGCTGACAAGTACGGTCCAATCTTTACCATATGGATCGGCAAGAACCGTACCCTGGTCATCAACAACTATGAAGCTGTCAAGGAATGTTTCACCACAAACGACAGACTTTTCGCCACAAGACCATTATCTGCACATGGAAAGTACCTTGGTTACGATTTTGCAGCATTCGGGTTCTCACCTTATGGCACATACTGGCGTGATATGAGAAAGTTGGTTGTGATTGAGTTGCTCTCTAATCGTAGGCTCGAGACTCTGAAACATGTACAAGTCTCTGAGGTTGATGCTTTTGTTAAAGGGTTGTATTCAGTTTGGAAAAACAAAGGACATGATGGTCAAAGCAAGGTGGTGATCAGTGAGTGGACTGAGCACCTAACCTTGAACGTGATCACTAGAGTTATTGCTGGGAAGAGGTACTTTGGTAAAGAGAGTGATGAACTTGATGAAGAGGAAAAGAGGATTGGGAAGCTTATCAAGGACTTTATGTATGTTTCTGGCAACCCAGTTGTTACTGAGGTAATTGGGTTTCCAAAGTGGATGGATTTCAAGGGGCAATTGAAAACGATGGAGAATATAGGGAGCCAATTAGACTCTTTGATGACAAGTTGGGTTGAAGAACATCATACAAAGAAGCAGAAGAGTGACTCAAGCAACCAGCAGCAGGACTTCATTAATGTCCTACTATCTGAGATTCAGGACAGTTCTATGCTTGGTCATACCCGAGATACTATTATCAAGGCAACAGCACTGAATCTCATCCTAGCCGGTTCAGAATCCATATCAATCAGCTTGACATGGACTGTTATACACTTGACGCTGGCAAGGTTGATTCAAGGGTTTGAATTGGCAACTCCATTGGATAAGGCAGTGGATATGACTGAAGGATTGGGGATCACTTTACCAAGAGCAACTCCACTGGAAGTTCTCTTAACCCCACGTTTAGCTTCTGAATTGTATCCACAGTAA

Protein Analysis

385

Amino Acids

43.63

Weight (kDa)

8.32

Isoelectric Point (pI)

30.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 38 - 336 7.2e-35 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 525
AccB1I GGYRCC 1 cut(s) 122
AclWI GGATC 2 cut(s) 236, 1091
AcoI YGGCCR 1 cut(s) 160
AcsI RAATTY 1 cut(s) 36
AcuI CTGAAG 1 cut(s) 1092
AfaI GTAC 5 cut(s) 206, 244, 338, 459, 608
AfiI CCNNNNNNNGG 3 cut(s) 137, 247, 853
AflIII ACRYGT 1 cut(s) 454
AgsI TTSAA 6 cut(s) 574, 739, 751, 806, 1025, 1034
AjnI CCWGG 1 cut(s) 246
AjuI GAANNNNNNNTTGG 2 cut(s) 636, 668
AluBI AGCT 4 cut(s) 269, 661, 981, 1136
AluI AGCT 4 cut(s) 269, 661, 981, 1136
Alw21I GWGCWC 1 cut(s) 564
Alw26I GTCTC 2 cut(s) 437, 469
AlwI GGATC 2 cut(s) 236, 1091
Ama87I CYCGRG 2 cut(s) 440, 912
AoxI GGCC 2 cut(s) 97, 160
ApeKI GCWGC 2 cut(s) 356, 850
ApoI RAATTY 1 cut(s) 36
ArsI GACNNNNNNTTYG 2 cut(s) 287, 319
AseI ATTAAT 1 cut(s) 864
AspS9I GGNCC 2 cut(s) 97, 209
AsuHPI GGTGA 5 cut(s) 82, 134, 277, 363, 553
AvaI CYCGRG 2 cut(s) 440, 912
AvaII GGWCC 1 cut(s) 209
BaeGI GKGCMC 2 cut(s) 110, 127
BaeI ACNNNNGTAYC 2 cut(s) 598, 631
BanI GGYRCC 1 cut(s) 122
Bbv12I GWGCWC 1 cut(s) 564
BbvI GCAGC 2 cut(s) 368, 862
BccI CCATC 4 cut(s) 156, 518, 724, 751
BciT130I CCWGG 1 cut(s) 248
BciVI GTATCC 1 cut(s) 1158
BclI TGATCA 2 cut(s) 543, 579
BcoDI GTCTC 2 cut(s) 437, 469
BfaI CTAG 5 cut(s) 23, 41, 155, 585, 953
BfuI GTATCC 1 cut(s) 1158
BisI GCNGC 2 cut(s) 357, 851
BlsI GCNGC 2 cut(s) 358, 852
Bme1390I CCNGG 1 cut(s) 248
Bme18I GGWCC 1 cut(s) 209
BmeT110I CYCGRG 2 cut(s) 440, 912
BmgT120I GGNCC 2 cut(s) 97, 209
BmiI GGNNCC 2 cut(s) 124, 773
BmrFI CCNGG 1 cut(s) 248
BmrI ACTGGG 2 cut(s) 164, 689
BmsI GCATC 1 cut(s) 466
BmuI ACTGGG 2 cut(s) 164, 689
BplI GAGNNNNNCTC 2 cut(s) 410, 442
BpuEI CTTGAG 1 cut(s) 823
BsaJI CCNNGG 3 cut(s) 192, 246, 340
Bsc4I CCNNNNNNNGG 3 cut(s) 137, 247, 853
Bse118I RCCGGY 1 cut(s) 956
Bse1I ACTGG 4 cut(s) 170, 392, 695, 1113
BseBI CCWGG 1 cut(s) 248
BseDI CCNNGG 3 cut(s) 192, 246, 340
BseGI GGATG 2 cut(s) 735, 948
BseLI CCNNNNNNNGG 3 cut(s) 137, 247, 853
BseMII CTCAG 4 cut(s) 459, 549, 696, 870
BseNI ACTGG 4 cut(s) 170, 392, 695, 1113
BseSI GKGCMC 2 cut(s) 110, 127
BseXI GCAGC 2 cut(s) 368, 862
BseYI CCCAGC 1 cut(s) 596
BsgI GTGCAG 1 cut(s) 309
BshFI GGCC 2 cut(s) 99, 162
BshNI GGYRCC 1 cut(s) 122
BsiHKAI GWGCWC 1 cut(s) 564
BsiHKCI CYCGRG 2 cut(s) 440, 912
BsiSI CCGG 1 cut(s) 957
BslI CCNNNNNNNGG 3 cut(s) 137, 247, 853
BsmAI GTCTC 2 cut(s) 437, 469
BsmI GAATGC 1 cut(s) 359
BsnI GGCC 2 cut(s) 99, 162
BsoBI CYCGRG 2 cut(s) 440, 912
Bsp1286I GDGCHC 3 cut(s) 110, 127, 564
Bsp1407I TGTACA 1 cut(s) 457
Bsp143I GATC 4 cut(s) 228, 543, 579, 1083
Bsp19I CCATGG 1 cut(s) 192
BspANI GGCC 2 cut(s) 99, 162
BspCNI CTCAG 4 cut(s) 460, 550, 697, 871
BspLI GGNNCC 2 cut(s) 124, 773
BspPI GGATC 2 cut(s) 236, 1091
BspT107I GGYRCC 1 cut(s) 122
BsrFI RCCGGY 1 cut(s) 956
BsrGI TGTACA 1 cut(s) 457
BsrI ACTGG 4 cut(s) 170, 392, 695, 1113
BssAI RCCGGY 1 cut(s) 956
BssECI CCNNGG 3 cut(s) 192, 246, 340
BssMI GATC 4 cut(s) 228, 543, 579, 1083
BssT1I CCWWGG 2 cut(s) 192, 340
Bst2UI CCWGG 1 cut(s) 248
Bst4CI ACNGT 5 cut(s) 209, 242, 893, 994, 1155
Bst6I CTCTTC 3 cut(s) 596, 633, 824
BstAUI TGTACA 1 cut(s) 457
BstC8I GCNNGC 1 cut(s) 1011
BstDEI CTNAG 4 cut(s) 468, 558, 705, 879
BstDSI CCRYGG 1 cut(s) 192
BstEII GGTNACC 1 cut(s) 140
BstF5I GGATG 2 cut(s) 735, 948
BstKTI GATC 4 cut(s) 231, 546, 582, 1086
BstMAI GTCTC 2 cut(s) 437, 469
BstMBI GATC 4 cut(s) 228, 543, 579, 1083
BstMWI GCNNNNNNNGC 1 cut(s) 105
BstNI CCWGG 1 cut(s) 248
BstNSI RCATGY 1 cut(s) 458
BstPI GGTNACC 1 cut(s) 140
BstSCI CCNGG 1 cut(s) 246
BstSLI GKGCMC 2 cut(s) 110, 127
BstV1I GCAGC 2 cut(s) 368, 862
BsuI GTATCC 1 cut(s) 1158
BsuRI GGCC 2 cut(s) 99, 162
BtgI CCRYGG 1 cut(s) 192
BtsCI GGATG 2 cut(s) 735, 948
BtsI GCAGTG 1 cut(s) 1065
BtsIMutI CAGTG 5 cut(s) 177, 553, 938, 1065, 1106
Cac8I GCNNGC 1 cut(s) 1011
Cfr10I RCCGGY 1 cut(s) 956
Cfr13I GGNCC 2 cut(s) 97, 209
CseI GACGC 1 cut(s) 1015
Csp6I GTAC 5 cut(s) 205, 243, 337, 458, 607
CviAII CATG 5 cut(s) 193, 329, 455, 521, 987
CviQI GTAC 5 cut(s) 205, 243, 337, 458, 607
DdeI CTNAG 4 cut(s) 468, 558, 705, 879
DpnI GATC 4 cut(s) 230, 545, 581, 1085
DpnII GATC 4 cut(s) 228, 543, 579, 1083
DrdI GACNNNNNNGTC 1 cut(s) 525
DseDI GACNNNNNNGTC 1 cut(s) 525
EaeI YGGCCR 1 cut(s) 160
Eam1104I CTCTTC 3 cut(s) 596, 633, 824
EarI CTCTTC 3 cut(s) 596, 633, 824
Eco130I CCWWGG 2 cut(s) 192, 340
Eco47I GGWCC 1 cut(s) 209
Eco57I CTGAAG 1 cut(s) 1092
Eco88I CYCGRG 2 cut(s) 440, 912
Eco91I GGTNACC 1 cut(s) 140
EcoO109I RGGNCCY 1 cut(s) 97
EcoO65I GGTNACC 1 cut(s) 140
EcoRI GAATTC 1 cut(s) 36
EcoRII CCWGG 1 cut(s) 246
EcoT14I CCWWGG 2 cut(s) 192, 340
ErhI CCWWGG 2 cut(s) 192, 340
FaeI CATG 5 cut(s) 196, 332, 458, 524, 990
FalI AAGNNNNNCTT 2 cut(s) 593, 625
FatI CATG 5 cut(s) 192, 328, 454, 520, 986
FauNDI CATATG 1 cut(s) 224
FbaI TGATCA 2 cut(s) 543, 579
Fnu4HI GCNGC 2 cut(s) 357, 851
FokI GGATG 2 cut(s) 742, 935
Fsp4HI GCNGC 2 cut(s) 357, 851
FspBI CTAG 5 cut(s) 23, 41, 155, 585, 953
GluI GCNGC 2 cut(s) 357, 851
GsaI CCCAGC 1 cut(s) 600
HaeIII GGCC 2 cut(s) 99, 162
HapII CCGG 1 cut(s) 957
HgaI GACGC 1 cut(s) 1015
Hin1II CATG 5 cut(s) 196, 332, 458, 524, 990
HindIII AAGCTT 1 cut(s) 659
HinfI GANTC 9 cut(s) 5, 82, 445, 782, 836, 883, 943, 965, 1021
HpaII CCGG 1 cut(s) 957
HphI GGTGA 5 cut(s) 82, 134, 277, 363, 553
Hpy166II GTNNAC 1 cut(s) 555
Hpy188I TCNGA 7 cut(s) 81, 179, 450, 469, 880, 964, 1141
Hpy188III TCNNGA 2 cut(s) 442, 887
Hpy8I GTNNAC 1 cut(s) 555
HpyAV CCTTC 2 cut(s) 88, 1067
HpyCH4III ACNGT 5 cut(s) 209, 242, 893, 994, 1155
HpyCH4IV ACGT 2 cut(s) 576, 1129
HpyCH4V TGCA 2 cut(s) 326, 356
HpyF10VI GCNNNNNNNGC 1 cut(s) 105
HpyF3I CTNAG 4 cut(s) 468, 558, 705, 879
HpySE526I ACGT 2 cut(s) 576, 1129
Hsp92II CATG 5 cut(s) 196, 332, 458, 524, 990
Ksp22I TGATCA 2 cut(s) 543, 579
Kzo9I GATC 4 cut(s) 228, 543, 579, 1083
LmnI GCTCC 1 cut(s) 771
Lsp1109I GCAGC 2 cut(s) 368, 862
LweI GCATC 1 cut(s) 466
MaeI CTAG 5 cut(s) 23, 41, 155, 585, 953
MaeII ACGT 2 cut(s) 576, 1129
MaeIII GTNAC 4 cut(s) 140, 344, 700, 833
MalI GATC 4 cut(s) 230, 545, 581, 1085
MboI GATC 4 cut(s) 228, 543, 579, 1083
MboII GAAGA 4 cut(s) 613, 650, 818, 841
MfeI CAATTG 1 cut(s) 746
MhlI GDGCHC 3 cut(s) 110, 127, 564
MluCI AATT 8 cut(s) 27, 36, 132, 711, 746, 776, 1034, 1142
MlyI GAGTC 3 cut(s) 439, 776, 830
MnlI CCTC 7 cut(s) 60, 155, 463, 597, 634, 642, 700
MseI TTAA 3 cut(s) 486, 864, 1121
MspI CCGG 1 cut(s) 957
MspR9I CCNGG 1 cut(s) 248
MunI CAATTG 1 cut(s) 746
Mva1269I GAATGC 1 cut(s) 359
MvaI CCWGG 1 cut(s) 248
MwoI GCNNNNNNNGC 1 cut(s) 105
NcoI CCATGG 1 cut(s) 192
NdeI CATATG 1 cut(s) 224
NdeII GATC 4 cut(s) 228, 543, 579, 1083
NlaIII CATG 5 cut(s) 196, 332, 458, 524, 990
NlaIV GGNNCC 2 cut(s) 124, 773
NmuCI GTSAC 2 cut(s) 140, 833
NspI RCATGY 1 cut(s) 458
PaeR7I CTCGAG 1 cut(s) 440
PciI ACATGT 1 cut(s) 454
PctI GAATGC 1 cut(s) 359
PfeI GAWTC 6 cut(s) 5, 82, 883, 943, 965, 1021
PkrI GCNGC 2 cut(s) 358, 852
PleI GAGTC 3 cut(s) 439, 776, 830
PpsI GAGTC 3 cut(s) 439, 776, 830
PscI ACATGT 1 cut(s) 454
PshBI ATTAAT 1 cut(s) 864
Psp6I CCWGG 1 cut(s) 246
PspEI GGTNACC 1 cut(s) 140
PspFI CCCAGC 1 cut(s) 596
PspGI CCWGG 1 cut(s) 246
PspN4I GGNNCC 2 cut(s) 124, 773
PspPI GGNCC 2 cut(s) 97, 209
RsaI GTAC 5 cut(s) 206, 244, 338, 459, 608
RsaNI GTAC 5 cut(s) 205, 243, 337, 458, 607
SaqAI TTAA 3 cut(s) 486, 864, 1121
SatI GCNGC 2 cut(s) 357, 851
Sau3AI GATC 4 cut(s) 228, 543, 579, 1083
Sau96I GGNCC 2 cut(s) 97, 209
SchI GAGTC 3 cut(s) 439, 776, 830
ScrFI CCNGG 1 cut(s) 248
SduI GDGCHC 3 cut(s) 110, 127, 564
SfaNI GCATC 1 cut(s) 466
Sfr274I CTCGAG 1 cut(s) 440
SinI GGWCC 1 cut(s) 209
SlaI CTCGAG 1 cut(s) 440
SmlI CTYRAG 2 cut(s) 440, 838
SmoI CTYRAG 2 cut(s) 440, 838
Sse9I AATT 8 cut(s) 27, 36, 132, 711, 746, 776, 1034, 1142
SspMI CTAG 5 cut(s) 23, 41, 155, 585, 953
StyD4I CCNGG 1 cut(s) 246
StyI CCWWGG 2 cut(s) 192, 340
TaaI ACNGT 5 cut(s) 209, 242, 893, 994, 1155
TaiI ACGT 2 cut(s) 579, 1132
TaqI TCGA 1 cut(s) 441
TasI AATT 8 cut(s) 27, 36, 132, 711, 746, 776, 1034, 1142
TatI WGTACW 1 cut(s) 457
TfiI GAWTC 6 cut(s) 5, 82, 883, 943, 965, 1021
Tru1I TTAA 3 cut(s) 486, 864, 1121
Tru9I TTAA 3 cut(s) 486, 864, 1121
TscAI CASTG 5 cut(s) 177, 553, 945, 1065, 1113
TseFI GTSAC 2 cut(s) 140, 833
TseI GCWGC 2 cut(s) 356, 850
Tsp45I GTSAC 2 cut(s) 140, 833
TspDTI ATGAA 5 cut(s) 107, 279, 642, 651, 850
TspRI CASTG 5 cut(s) 177, 553, 945, 1065, 1113
VpaK11BI GGWCC 1 cut(s) 209
VspI ATTAAT 1 cut(s) 864
XapI RAATTY 1 cut(s) 36
XceI RCATGY 1 cut(s) 458
XcmI CCANNNNNNNNNTGG 1 cut(s) 155
XhoI CTCGAG 1 cut(s) 440
XspI CTAG 5 cut(s) 23, 41, 155, 585, 953
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.