Rw6G023330

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0006974
Physical Location & Seq
Reverse (-)
45660888 .. 45663916
3029 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G023330.1

Sequence Viewer

Length: 1569 bp
ATGGATTATCTTTCTCATCAACTAGCAATTACAGGACTTCTAATTTTGGTTTCGTTGTATCTATGGAGGGTGAGATTTCAGAATCACAAAATGAAGGGCCTCATGTGCCCAGAACCCTATGGTGCCCTGCCAATTATAGGTCACCTCCATCAACTAGGTGGCCGAAACCCACTGTTTCGAACCTTAGCAACAATGGCTGACAAGTATGGTCCAATCTTTACCATATGGCTCGGCAAGAAGCGTGCCCTGGTGATCAACAACTACGAAGCTGTCAAGGAATACCAATTTCTGCACAGGGAGAGTACCTTGGATAATTATGCAGCATTCGGGTTCTCACCCTATGGCACATATTGGCGTGATATGAGAAAGTTGGTTGTGATCGAGTTGCTCTCTAATCGTAGGCTAGAGACTCTGAAACATGTACAAGTCTCTGAGGTTGATGCTTTTGTTAAAGGGTTGTATTCAGTTTGGGAAAACAAAGGACGTGATGGTCAAAGCAAGGTGGTGATCTGTGAATGGATTGAGCGCCTAACCTTGAACGTGATCACTAGAATTATTGCTGGGAAGAGGTACTTTGGTAAAGAGAGTGATGAACTTGATGGAGAGGAAAAGAGGATTGGGAAGCTTATGAAGGACTTTATGTATGCTGCTGGCAAACCAGTTGTTTCTGATGTAATTGGGTTTCCAAGGTGGATGGATTTCAAGGGACAATTGAAAACTATGAAGAATATAGGGAGCCAATTAGACTCTTTGATGACAAGTTGGGTTGAAGAACATCATACAAAGAAGCAGAAGAGTGACTCAAGCAACCAGCAGCAGGACTTCATTAATGTCCTACTATCTGAGATTCAGTACAGTTCTATGCTTGGTCATACCCGAGATACTATTATCAAGGCAACAGCACTGGTGAACCGTCACAATGGTAGGCCAAAATCGTTGATTACCCCCACACTTGCCGGTTCAGAATCCACATCAATCAGCTTGACATGGACCGTCTCTTTACTATTAAACAACAGACATATCTTGAAGCTAGCCCAAGAGGAATTAGATCGAACAGTTGGCAGGCACAGATGGGTGGAAGAAACTGACATTAAAAACCTGGTTTACTTAAAAGCCATTGTCAAAGAAACATTGCGTCTATACCCACCAGCTCCACTCTCAGTTCCACATGAAGCAATGGAGGACTGCAAAGTCGGCGGCTTTGACATCCCGAAGGGCACTCTTTTGTTTGTGAATCTGTGGAAGCTTCATAGGAACCCAAGTGTGTGGTCAGACCCTGAAGTGTTTTCCCCAGAAAGATTTCTTACAAGCCAAGCAGGTGTAGACGCTTCAGGTCAACATTTTGAGTTCATTCCTTTTGGGTCTGGCAGAAGAGTTTGCCCAGGTATCACTTTTGCGTTTCAGCTTATACACTTGACGCTGGCAAGGTTGATTCAAGGGTTTGAATTGGCAACTCCATTGGATAAGGCAGTGGATATGACTGAAGGATTGGGGATCACTATACCAAGAGCAACTCCACTGGAAGTTCTCTTGACCCCACGTTTAGCTTTTGAGTTGTATCCACAGTAA

Protein Analysis

522

Amino Acids

59.48

Weight (kDa)

9.12

Isoelectric Point (pI)

35.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 38 - 502 1.4e-86 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1307
AasI GACNNNNNNGTC 2 cut(s) 489, 1190
Acc36I ACCTGC 1 cut(s) 1307
AccB1I GGYRCC 1 cut(s) 122
AccI GTMKAC 1 cut(s) 1323
AciI CCGC 1 cut(s) 1197
AclWI GGATC 1 cut(s) 1502
AcoI YGGCCR 1 cut(s) 160
AcuI CTGAAG 3 cut(s) 1299, 1314, 1503
AfaI GTAC 4 cut(s) 304, 423, 572, 854
AfiI CCNNNNNNNGG 2 cut(s) 137, 817
AflIII ACRYGT 1 cut(s) 418
AgsI TTSAA 7 cut(s) 538, 703, 715, 770, 1027, 1436, 1445
AjiI CACGTC 1 cut(s) 485
AjnI CCWGG 3 cut(s) 246, 1098, 1381
AjuI GAANNNNNNNTTGG 2 cut(s) 600, 632
AluBI AGCT 8 cut(s) 269, 625, 981, 1030, 1151, 1246, 1405, 1547
AluI AGCT 8 cut(s) 269, 625, 981, 1030, 1151, 1246, 1405, 1547
Alw26I GTCTC 3 cut(s) 401, 433, 1000
AlwI GGATC 1 cut(s) 1502
AlwNI CAGNNNCTG 1 cut(s) 1277
Ama87I CYCGRG 1 cut(s) 876
AoxI GGCC 3 cut(s) 97, 160, 926
ApeKI GCWGC 3 cut(s) 320, 647, 814
AseI ATTAAT 1 cut(s) 828
Asp700I GAANNNNTTC 1 cut(s) 1299
AspLEI GCGC 1 cut(s) 528
AspS9I GGNCC 3 cut(s) 97, 209, 990
AsuHPI GGTGA 6 cut(s) 82, 134, 262, 327, 517, 919
AsuII TTCGAA 1 cut(s) 178
AsuNHI GCTAGC 1 cut(s) 1030
AvaI CYCGRG 1 cut(s) 876
AvaII GGWCC 2 cut(s) 209, 990
BaeGI GKGCMC 4 cut(s) 110, 127, 247, 1220
BaeI ACNNNNGTAYC 2 cut(s) 562, 595
BanI GGYRCC 1 cut(s) 122
BbvI GCAGC 3 cut(s) 332, 634, 826
BccI CCATC 5 cut(s) 156, 482, 593, 688, 1065
BciT130I CCWGG 3 cut(s) 248, 1100, 1383
BciVI GTATCC 1 cut(s) 1569
BclI TGATCA 2 cut(s) 252, 543
BcoDI GTCTC 3 cut(s) 401, 433, 1000
BfaI CTAG 5 cut(s) 23, 155, 404, 549, 1031
BfoI RGCGCY 1 cut(s) 529
BfuAI ACCTGC 1 cut(s) 1307
BfuI GTATCC 1 cut(s) 1569
BisI GCNGC 4 cut(s) 321, 648, 815, 1198
BlsI GCNGC 4 cut(s) 322, 649, 816, 1199
Bme1390I CCNGG 3 cut(s) 248, 1100, 1383
Bme18I GGWCC 2 cut(s) 209, 990
BmeT110I CYCGRG 1 cut(s) 876
BmgBI CACGTC 1 cut(s) 485
BmgT120I GGNCC 3 cut(s) 97, 209, 990
BmiI GGNNCC 3 cut(s) 124, 737, 1256
BmrFI CCNGG 3 cut(s) 248, 1100, 1383
BmsI GCATC 1 cut(s) 430
BmtI GCTAGC 1 cut(s) 1034
BplI GAGNNNNNCTC 2 cut(s) 374, 406
Bpu10I CCTNAGC 1 cut(s) 184
Bpu14I TTCGAA 1 cut(s) 178
BpuEI CTTGAG 1 cut(s) 787
BsaJI CCNNGG 4 cut(s) 246, 306, 686, 1381
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 817
Bse118I RCCGGY 1 cut(s) 956
Bse1I ACTGG 3 cut(s) 659, 909, 1524
Bse3DI GCAATG 2 cut(s) 1130, 1182
BseBI CCWGG 3 cut(s) 248, 1100, 1383
BseDI CCNNGG 4 cut(s) 246, 306, 686, 1381
BseGI GGATG 2 cut(s) 699, 1206
BseLI CCNNNNNNNGG 2 cut(s) 137, 817
BseMI GCAATG 2 cut(s) 1130, 1182
BseMII CTCAG 3 cut(s) 423, 834, 1173
BseNI ACTGG 3 cut(s) 659, 909, 1524
BseSI GKGCMC 4 cut(s) 110, 127, 247, 1220
BseXI GCAGC 3 cut(s) 332, 634, 826
BseYI CCCAGC 1 cut(s) 560
BsgI GTGCAG 1 cut(s) 275
BshFI GGCC 3 cut(s) 99, 162, 928
BshNI GGYRCC 1 cut(s) 122
BsiHKCI CYCGRG 1 cut(s) 876
BsiSI CCGG 1 cut(s) 957
BslFI GGGAC 1 cut(s) 720
BslI CCNNNNNNNGG 2 cut(s) 137, 817
BsmAI GTCTC 3 cut(s) 401, 433, 1000
BsmBI CGTCTC 1 cut(s) 1000
BsmFI GGGAC 1 cut(s) 720
BsmI GAATGC 1 cut(s) 323
BsnI GGCC 3 cut(s) 99, 162, 928
BsoBI CYCGRG 1 cut(s) 876
Bsp119I TTCGAA 1 cut(s) 178
Bsp1286I GDGCHC 4 cut(s) 110, 127, 247, 1220
Bsp1407I TGTACA 1 cut(s) 421
Bsp143I GATC 6 cut(s) 252, 378, 507, 543, 1048, 1494
BspACI CCGC 1 cut(s) 1197
BspANI GGCC 3 cut(s) 99, 162, 928
BspCNI CTCAG 3 cut(s) 424, 835, 1172
BspLI GGNNCC 3 cut(s) 124, 737, 1256
BspMI ACCTGC 1 cut(s) 1307
BspOI GCTAGC 1 cut(s) 1034
BspPI GGATC 1 cut(s) 1502
BspT104I TTCGAA 1 cut(s) 178
BspT107I GGYRCC 1 cut(s) 122
BsrDI GCAATG 2 cut(s) 1130, 1182
BsrFI RCCGGY 1 cut(s) 956
BsrGI TGTACA 1 cut(s) 421
BsrI ACTGG 3 cut(s) 659, 909, 1524
BssAI RCCGGY 1 cut(s) 956
BssECI CCNNGG 4 cut(s) 246, 306, 686, 1381
BssMI GATC 6 cut(s) 252, 378, 507, 543, 1048, 1494
BssT1I CCWWGG 2 cut(s) 306, 686
Bst2UI CCWGG 3 cut(s) 248, 1100, 1383
Bst4CI ACNGT 6 cut(s) 174, 857, 914, 994, 1057, 1566
Bst6I CTCTTC 3 cut(s) 560, 788, 1366
BstAUI TGTACA 1 cut(s) 421
BstBI TTCGAA 1 cut(s) 178
BstC8I GCNNGC 5 cut(s) 243, 652, 1032, 1064, 1422
BstDEI CTNAG 4 cut(s) 184, 432, 843, 1159
BstEII GGTNACC 1 cut(s) 140
BstF5I GGATG 2 cut(s) 699, 1206
BstH2I RGCGCY 1 cut(s) 529
BstHHI GCGC 1 cut(s) 528
BstKTI GATC 6 cut(s) 255, 381, 510, 546, 1051, 1497
BstMAI GTCTC 3 cut(s) 401, 433, 1000
BstMBI GATC 6 cut(s) 252, 378, 507, 543, 1048, 1494
BstMWI GCNNNNNNNGC 3 cut(s) 105, 194, 1194
BstNI CCWGG 3 cut(s) 248, 1100, 1383
BstNSI RCATGY 1 cut(s) 422
BstPI GGTNACC 1 cut(s) 140
BstSCI CCNGG 3 cut(s) 246, 1098, 1381
BstSLI GKGCMC 4 cut(s) 110, 127, 247, 1220
BstV1I GCAGC 3 cut(s) 332, 634, 826
BstXI CCANNNNNNTGG 1 cut(s) 1266
BsuI GTATCC 1 cut(s) 1569
BsuRI GGCC 3 cut(s) 99, 162, 928
BtrI CACGTC 1 cut(s) 485
BtsCI GGATG 2 cut(s) 699, 1206
BtsI GCAGTG 1 cut(s) 1476
BtsIMutI CAGTG 4 cut(s) 170, 902, 1476, 1517
BveI ACCTGC 1 cut(s) 1307
Cac8I GCNNGC 5 cut(s) 243, 652, 1032, 1064, 1422
CaiI CAGNNNCTG 1 cut(s) 1277
CfoI GCGC 1 cut(s) 528
Cfr10I RCCGGY 1 cut(s) 956
Cfr13I GGNCC 3 cut(s) 97, 209, 990
CseI GACGC 3 cut(s) 1124, 1334, 1426
CsiI ACCWGGT 1 cut(s) 1098
Csp6I GTAC 4 cut(s) 303, 422, 571, 853
CviAII CATG 4 cut(s) 103, 419, 987, 1169
CviQI GTAC 4 cut(s) 303, 422, 571, 853
DdeI CTNAG 4 cut(s) 184, 432, 843, 1159
DpnI GATC 6 cut(s) 254, 380, 509, 545, 1050, 1496
DpnII GATC 6 cut(s) 252, 378, 507, 543, 1048, 1494
DrdI GACNNNNNNGTC 2 cut(s) 489, 1190
DseDI GACNNNNNNGTC 2 cut(s) 489, 1190
EaeI YGGCCR 1 cut(s) 160
Eam1104I CTCTTC 3 cut(s) 560, 788, 1366
EarI CTCTTC 3 cut(s) 560, 788, 1366
Eco130I CCWWGG 2 cut(s) 306, 686
Eco47I GGWCC 2 cut(s) 209, 990
Eco57I CTGAAG 3 cut(s) 1299, 1314, 1503
Eco88I CYCGRG 1 cut(s) 876
Eco91I GGTNACC 1 cut(s) 140
EcoO109I RGGNCCY 1 cut(s) 97
EcoO65I GGTNACC 1 cut(s) 140
EcoRII CCWGG 3 cut(s) 246, 1098, 1381
EcoT14I CCWWGG 2 cut(s) 306, 686
ErhI CCWWGG 2 cut(s) 306, 686
Esp3I CGTCTC 1 cut(s) 1000
FaeI CATG 4 cut(s) 106, 422, 990, 1172
FalI AAGNNNNNCTT 2 cut(s) 557, 589
FaqI GGGAC 1 cut(s) 720
FatI CATG 4 cut(s) 102, 418, 986, 1168
FauNDI CATATG 1 cut(s) 224
FbaI TGATCA 2 cut(s) 252, 543
FblI GTMKAC 1 cut(s) 1323
Fnu4HI GCNGC 4 cut(s) 321, 648, 815, 1198
FokI GGATG 2 cut(s) 706, 1193
Fsp4HI GCNGC 4 cut(s) 321, 648, 815, 1198
FspBI CTAG 5 cut(s) 23, 155, 404, 549, 1031
GlaI GCGC 1 cut(s) 527
GluI GCNGC 4 cut(s) 321, 648, 815, 1198
GsaI CCCAGC 1 cut(s) 564
HaeII RGCGCY 1 cut(s) 529
HaeIII GGCC 3 cut(s) 99, 162, 928
HapII CCGG 1 cut(s) 957
HgaI GACGC 3 cut(s) 1124, 1334, 1426
HhaI GCGC 1 cut(s) 528
Hin1II CATG 4 cut(s) 106, 422, 990, 1172
Hin6I GCGC 1 cut(s) 526
HinP1I GCGC 1 cut(s) 526
HincII GTYRAC 1 cut(s) 1337
HindII GTYRAC 1 cut(s) 1337
HindIII AAGCTT 2 cut(s) 623, 1244
HinfI GANTC 8 cut(s) 82, 409, 746, 800, 847, 965, 1234, 1432
HpaII CCGG 1 cut(s) 957
HphI GGTGA 6 cut(s) 82, 134, 262, 327, 517, 919
Hpy166II GTNNAC 4 cut(s) 910, 1105, 1324, 1337
Hpy188I TCNGA 7 cut(s) 81, 414, 433, 670, 844, 964, 1273
Hpy188III TCNNGA 3 cut(s) 1024, 1210, 1531
Hpy8I GTNNAC 4 cut(s) 910, 1105, 1324, 1337
HpyAV CCTTC 4 cut(s) 88, 625, 1207, 1478
HpyCH4III ACNGT 6 cut(s) 174, 857, 914, 994, 1057, 1566
HpyCH4IV ACGT 3 cut(s) 484, 540, 1540
HpyCH4V TGCA 3 cut(s) 292, 320, 1188
HpyF10VI GCNNNNNNNGC 3 cut(s) 105, 194, 1194
HpyF3I CTNAG 4 cut(s) 184, 432, 843, 1159
HpySE526I ACGT 3 cut(s) 484, 540, 1540
Hsp92II CATG 4 cut(s) 106, 422, 990, 1172
HspAI GCGC 1 cut(s) 526
Ksp22I TGATCA 2 cut(s) 252, 543
Kzo9I GATC 6 cut(s) 252, 378, 507, 543, 1048, 1494
LmnI GCTCC 2 cut(s) 735, 1156
Lsp1109I GCAGC 3 cut(s) 332, 634, 826
LweI GCATC 1 cut(s) 430
MabI ACCWGGT 1 cut(s) 1098
MaeI CTAG 5 cut(s) 23, 155, 404, 549, 1031
MaeII ACGT 3 cut(s) 484, 540, 1540
MaeIII GTNAC 3 cut(s) 140, 797, 914
MalI GATC 6 cut(s) 254, 380, 509, 545, 1050, 1496
MboI GATC 6 cut(s) 252, 378, 507, 543, 1048, 1494
MboII GAAGA 6 cut(s) 577, 736, 782, 805, 1091, 1383
MfeI CAATTG 1 cut(s) 710
MhlI GDGCHC 4 cut(s) 110, 127, 247, 1220
MlyI GAGTC 3 cut(s) 403, 740, 794
MnlI CCTC 9 cut(s) 60, 110, 155, 427, 561, 598, 606, 1033, 1174
MroXI GAANNNNTTC 1 cut(s) 1299
MseI TTAA 5 cut(s) 450, 828, 1007, 1092, 1109
MspI CCGG 1 cut(s) 957
MspR9I CCNGG 3 cut(s) 248, 1100, 1383
MunI CAATTG 1 cut(s) 710
Mva1269I GAATGC 1 cut(s) 323
MvaI CCWGG 3 cut(s) 248, 1100, 1383
MwoI GCNNNNNNNGC 3 cut(s) 105, 194, 1194
NdeI CATATG 1 cut(s) 224
NdeII GATC 6 cut(s) 252, 378, 507, 543, 1048, 1494
NheI GCTAGC 1 cut(s) 1030
NlaIII CATG 4 cut(s) 106, 422, 990, 1172
NlaIV GGNNCC 3 cut(s) 124, 737, 1256
NmeAIII GCCGAG 1 cut(s) 210
NmuCI GTSAC 3 cut(s) 140, 797, 914
NspI RCATGY 1 cut(s) 422
NspV TTCGAA 1 cut(s) 178
PaqCI CACCTGC 1 cut(s) 1307
PciI ACATGT 1 cut(s) 418
PctI GAATGC 1 cut(s) 323
PdmI GAANNNNTTC 1 cut(s) 1299
PfeI GAWTC 5 cut(s) 82, 847, 965, 1234, 1432
PkrI GCNGC 4 cut(s) 322, 649, 816, 1199
PleI GAGTC 3 cut(s) 403, 740, 794
PpsI GAGTC 3 cut(s) 403, 740, 794
PscI ACATGT 1 cut(s) 418
PshBI ATTAAT 1 cut(s) 828
Psp6I CCWGG 3 cut(s) 246, 1098, 1381
PspEI GGTNACC 1 cut(s) 140
PspFI CCCAGC 1 cut(s) 560
PspGI CCWGG 3 cut(s) 246, 1098, 1381
PspN4I GGNNCC 3 cut(s) 124, 737, 1256
PspPI GGNCC 3 cut(s) 97, 209, 990
PstNI CAGNNNCTG 1 cut(s) 1277
RsaI GTAC 4 cut(s) 304, 423, 572, 854
RsaNI GTAC 4 cut(s) 303, 422, 571, 853
SaqAI TTAA 5 cut(s) 450, 828, 1007, 1092, 1109
SatI GCNGC 4 cut(s) 321, 648, 815, 1198
Sau3AI GATC 6 cut(s) 252, 378, 507, 543, 1048, 1494
Sau96I GGNCC 3 cut(s) 97, 209, 990
SchI GAGTC 3 cut(s) 403, 740, 794
ScrFI CCNGG 3 cut(s) 248, 1100, 1383
SduI GDGCHC 4 cut(s) 110, 127, 247, 1220
SexAI ACCWGGT 1 cut(s) 1098
SfaNI GCATC 1 cut(s) 430
SfuI TTCGAA 1 cut(s) 178
SinI GGWCC 2 cut(s) 209, 990
SmlI CTYRAG 1 cut(s) 802
SmoI CTYRAG 1 cut(s) 802
SsiI CCGC 1 cut(s) 1197
SspMI CTAG 5 cut(s) 23, 155, 404, 549, 1031
StyD4I CCNGG 3 cut(s) 246, 1098, 1381
StyI CCWWGG 2 cut(s) 306, 686
TaaI ACNGT 6 cut(s) 174, 857, 914, 994, 1057, 1566
TaiI ACGT 3 cut(s) 487, 543, 1543
TaqI TCGA 3 cut(s) 178, 381, 1051
TatI WGTACW 2 cut(s) 421, 852
TauI GCSGC 1 cut(s) 1200
TfiI GAWTC 5 cut(s) 82, 847, 965, 1234, 1432
Tru1I TTAA 5 cut(s) 450, 828, 1007, 1092, 1109
Tru9I TTAA 5 cut(s) 450, 828, 1007, 1092, 1109
TscAI CASTG 4 cut(s) 177, 909, 1476, 1524
TseFI GTSAC 3 cut(s) 140, 797, 914
TseI GCWGC 3 cut(s) 320, 647, 814
Tsp45I GTSAC 3 cut(s) 140, 797, 914
TspDTI ATGAA 8 cut(s) 107, 606, 644, 737, 814, 1185, 1238, 1339
TspRI CASTG 4 cut(s) 177, 909, 1476, 1524
VpaK11BI GGWCC 2 cut(s) 209, 990
VspI ATTAAT 1 cut(s) 828
XceI RCATGY 1 cut(s) 422
XcmI CCANNNNNNNNNTGG 1 cut(s) 155
XmiI GTMKAC 1 cut(s) 1323
XmnI GAANNNNTTC 1 cut(s) 1299
XspI CTAG 5 cut(s) 23, 155, 404, 549, 1031
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.