RLG00000012789

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0006974
Physical Location & Seq
Reverse (-)
22385586 .. 22387748
2163 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012789

Sequence Viewer

Length: 1479 bp
ATGAAGGGCCTTATGTGCCCAGAACCCTATGGTGCCCTGCCAATTATAGGTCACCTCCATCAACTAGGTGGCCGAAACCCACTGTTCCGAACCCTATCAACCATGGCTGACAAGTACGGTCCAATCTTTACCATATGGATCGGCAAGAACCGTACCCTGGTCATCAACAACTATGAAGCTGTCAAGGAATGTTTCACGACAAACGACAGACTTTTCGCCACAAGACCATTATCTGCACATGGAAAGTACCTTGGTTACGATTTTGCAGCATTCGGGTTCTCACCTTATGGCACATACTGGCGTGATATGAGAAAGTTGGTTGTGATTGAGTTGCTCTCTAATCGTAGGCTAGAGACTCTGAAACATGTACAAGTCTCTGAGGTTGGTGCTTTTGTTAAAGGGTTGTATTCAGTTTGGAAAAACAAAGGACATGATGGTCAAAGCAAGGTGGTGATCAGTGAGTGGACTGAGCACCTAACCTTGAACGTGATCACTAGAGTTATTGCTGGGAAGAGGTACTTTGGTAAAGAGAGTGATGAACTAGATGAAGAGGAAAAGAGGATTGGGAAGCTTATCAAGGACTTTATGTATGTTTCTGGCAAACCAGTTGTTACTGAGGTAATTGGGCTTCCAAAGTGGATGGATTTCAAGGGGCAATTGAAAACGATGGAGAACATAGGGAGCCAATTAGACTCTTTGATGACAACTTGGGTTGAAGAACATCATACAAAGATGCAGAAGAGAGACTCAAGCAACCAGCAGCAGGACTTCATTAGTGTCCTACTATCTGAGATTCAGGACAGTTCTATGCTTGGTCATACCCGAGATACTATTATCAAGGCAACAGCACTGAATCTCATCCTAGCCGGTTCAGAATCCATATCAATCAGCTTGACATGGACTGTCTCTTTACTATTAAACAACAGAGATACCTTGAAGCTAGCCCAAGAGGAATTAAATCGTACAGTTGGCAGGCACAGATGGGTGGAAGACACCGACATTAAAAACCTGGTTTACTTACAAGCCATCGTCAAAGAAACACTGCGTCTATACCCACCAGCTCCGCTCTCAGTTCCACATGAAGCAATGGAGGACTGCAAAGTCGGCGGCTTTCACATCCCAAAGGGCACTTGCTTGTTTGTGAATCTGTGGAAGCTTCATAGGAACCCAAGTGTGTGGCCAGACCCTGAAGTGTTTTCCCCAGAAAGATTTCTTACAAGCCAAGCAGGCGTAGACGCTTCAGGTCAACATTTTGAGTTCATTCCTTTTGGGTCTGGGAGAAGAGTTTGCCCAGGTGTCACTTTTGCATTTCAGGTTATACACTTGACGCTGGCAAGGTTGATTCAAGGGTTTGAATTGGCAACTCCATTGGATAAGGCAGTGGATATGACTGAAGGATCGGGGATCACTTTACCAAGAGCAACTCCACTGGAAGTTCTCTTAACCCCACGTTTAGCTTCTGAATTGTATCCACAGTAA

Protein Analysis

493

Amino Acids

55.59

Weight (kDa)

8.13

Isoelectric Point (pI)

33.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 8 - 473 3e-96 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 435, 1100
AccB1I GGYRCC 1 cut(s) 32
AccBSI CCGCTC 1 cut(s) 1066
AccI GTMKAC 1 cut(s) 1233
AciI CCGC 2 cut(s) 1064, 1107
AclWI GGATC 3 cut(s) 146, 1405, 1412
AcoI YGGCCR 2 cut(s) 70, 1178
AcuI CTGAAG 3 cut(s) 1209, 1224, 1413
AfaI GTAC 6 cut(s) 116, 154, 248, 369, 518, 964
AfiI CCNNNNNNNGG 3 cut(s) 47, 157, 763
AflIII ACRYGT 1 cut(s) 364
AgsI TTSAA 7 cut(s) 484, 649, 661, 716, 937, 1346, 1355
AjnI CCWGG 3 cut(s) 156, 1008, 1291
AjuI GAANNNNNNNTTGG 2 cut(s) 546, 578
AluBI AGCT 7 cut(s) 179, 571, 891, 940, 1061, 1156, 1457
AluI AGCT 7 cut(s) 179, 571, 891, 940, 1061, 1156, 1457
Alw21I GWGCWC 1 cut(s) 474
Alw26I GTCTC 4 cut(s) 347, 379, 738, 910
AlwI GGATC 3 cut(s) 146, 1405, 1412
AlwNI CAGNNNCTG 1 cut(s) 1187
Ama87I CYCGRG 1 cut(s) 822
AoxI GGCC 3 cut(s) 7, 70, 1178
ApeKI GCWGC 2 cut(s) 266, 760
ArsI GACNNNNNNTTYG 2 cut(s) 197, 229
Asp700I GAANNNNTTC 1 cut(s) 1209
AspS9I GGNCC 2 cut(s) 7, 119
AsuHPI GGTGA 3 cut(s) 44, 273, 463
AsuNHI GCTAGC 1 cut(s) 940
AvaI CYCGRG 1 cut(s) 822
AvaII GGWCC 1 cut(s) 119
BaeGI GKGCMC 3 cut(s) 20, 37, 1130
BaeI ACNNNNGTAYC 2 cut(s) 508, 541
BalI TGGCCA 1 cut(s) 1180
BanI GGYRCC 1 cut(s) 32
BarI GAAGNNNNNNTAC 2 cut(s) 612, 644
BbsI GAAGAC 1 cut(s) 996
Bbv12I GWGCWC 1 cut(s) 474
BbvI GCAGC 2 cut(s) 278, 772
BccI CCATC 6 cut(s) 66, 428, 634, 661, 975, 1034
BciT130I CCWGG 3 cut(s) 158, 1010, 1293
BciVI GTATCC 1 cut(s) 1479
BclI TGATCA 2 cut(s) 453, 489
BcoDI GTCTC 4 cut(s) 347, 379, 738, 910
BfaI CTAG 6 cut(s) 65, 350, 495, 542, 863, 941
BfuI GTATCC 1 cut(s) 1479
BglI GCCNNNNNGGC 1 cut(s) 1227
BisI GCNGC 3 cut(s) 267, 761, 1108
BlsI GCNGC 3 cut(s) 268, 762, 1109
Bme1390I CCNGG 3 cut(s) 158, 1010, 1293
Bme18I GGWCC 1 cut(s) 119
BmeT110I CYCGRG 1 cut(s) 822
BmgT120I GGNCC 2 cut(s) 7, 119
BmiI GGNNCC 3 cut(s) 34, 683, 1166
BmrFI CCNGG 3 cut(s) 158, 1010, 1293
BmsI GCATC 1 cut(s) 723
BmtI GCTAGC 1 cut(s) 944
BpiI GAAGAC 1 cut(s) 996
BplI GAGNNNNNCTC 2 cut(s) 320, 352
BpuEI CTTGAG 1 cut(s) 733
BsaJI CCNNGG 4 cut(s) 102, 156, 250, 1291
Bsc4I CCNNNNNNNGG 3 cut(s) 47, 157, 763
Bse118I RCCGGY 1 cut(s) 866
Bse1I ACTGG 3 cut(s) 302, 605, 1434
Bse3DI GCAATG 1 cut(s) 1092
BseBI CCWGG 3 cut(s) 158, 1010, 1293
BseDI CCNNGG 4 cut(s) 102, 156, 250, 1291
BseGI GGATG 3 cut(s) 645, 858, 1116
BseLI CCNNNNNNNGG 3 cut(s) 47, 157, 763
BseMI GCAATG 1 cut(s) 1092
BseMII CTCAG 5 cut(s) 369, 459, 606, 780, 1083
BseNI ACTGG 3 cut(s) 302, 605, 1434
BseSI GKGCMC 3 cut(s) 20, 37, 1130
BseXI GCAGC 2 cut(s) 278, 772
BseYI CCCAGC 1 cut(s) 506
BsgI GTGCAG 1 cut(s) 219
BshFI GGCC 3 cut(s) 9, 72, 1180
BshNI GGYRCC 1 cut(s) 32
BsiHKAI GWGCWC 1 cut(s) 474
BsiHKCI CYCGRG 1 cut(s) 822
BsiSI CCGG 1 cut(s) 867
BslI CCNNNNNNNGG 3 cut(s) 47, 157, 763
BsmAI GTCTC 4 cut(s) 347, 379, 738, 910
BsmI GAATGC 1 cut(s) 269
BsnI GGCC 3 cut(s) 9, 72, 1180
BsoBI CYCGRG 1 cut(s) 822
Bsp1286I GDGCHC 4 cut(s) 20, 37, 474, 1130
Bsp1407I TGTACA 1 cut(s) 367
Bsp143I GATC 5 cut(s) 138, 453, 489, 1397, 1404
Bsp19I CCATGG 1 cut(s) 102
BspACI CCGC 2 cut(s) 1064, 1107
BspANI GGCC 3 cut(s) 9, 72, 1180
BspCNI CTCAG 5 cut(s) 370, 460, 607, 781, 1082
BspLI GGNNCC 3 cut(s) 34, 683, 1166
BspOI GCTAGC 1 cut(s) 944
BspPI GGATC 3 cut(s) 146, 1405, 1412
BspT107I GGYRCC 1 cut(s) 32
BsrBI CCGCTC 1 cut(s) 1066
BsrDI GCAATG 1 cut(s) 1092
BsrFI RCCGGY 1 cut(s) 866
BsrGI TGTACA 1 cut(s) 367
BsrI ACTGG 3 cut(s) 302, 605, 1434
BssAI RCCGGY 1 cut(s) 866
BssECI CCNNGG 4 cut(s) 102, 156, 250, 1291
BssMI GATC 5 cut(s) 138, 453, 489, 1397, 1404
BssT1I CCWWGG 2 cut(s) 102, 250
Bst2UI CCWGG 3 cut(s) 158, 1010, 1293
Bst4CI ACNGT 7 cut(s) 84, 119, 152, 803, 904, 967, 1476
Bst6I CTCTTC 4 cut(s) 506, 543, 734, 1276
BstAUI TGTACA 1 cut(s) 367
BstC8I GCNNGC 4 cut(s) 942, 974, 1228, 1332
BstDEI CTNAG 5 cut(s) 378, 468, 615, 789, 1069
BstDSI CCRYGG 1 cut(s) 102
BstEII GGTNACC 1 cut(s) 50
BstF5I GGATG 3 cut(s) 645, 858, 1116
BstKTI GATC 5 cut(s) 141, 456, 492, 1400, 1407
BstMAI GTCTC 4 cut(s) 347, 379, 738, 910
BstMBI GATC 5 cut(s) 138, 453, 489, 1397, 1404
BstMWI GCNNNNNNNGC 3 cut(s) 15, 1104, 1227
BstNI CCWGG 3 cut(s) 158, 1010, 1293
BstNSI RCATGY 1 cut(s) 368
BstPI GGTNACC 1 cut(s) 50
BstSCI CCNGG 3 cut(s) 156, 1008, 1291
BstSLI GKGCMC 3 cut(s) 20, 37, 1130
BstV1I GCAGC 2 cut(s) 278, 772
BstV2I GAAGAC 1 cut(s) 996
BstXI CCANNNNNNTGG 1 cut(s) 1176
BsuI GTATCC 1 cut(s) 1479
BsuRI GGCC 3 cut(s) 9, 72, 1180
BtgI CCRYGG 1 cut(s) 102
BtsCI GGATG 3 cut(s) 645, 858, 1116
BtsI GCAGTG 2 cut(s) 1040, 1386
BtsIMutI CAGTG 6 cut(s) 80, 463, 848, 1040, 1386, 1427
Cac8I GCNNGC 4 cut(s) 942, 974, 1228, 1332
CaiI CAGNNNCTG 1 cut(s) 1187
Cfr10I RCCGGY 1 cut(s) 866
Cfr13I GGNCC 2 cut(s) 7, 119
CseI GACGC 3 cut(s) 1034, 1244, 1336
CsiI ACCWGGT 1 cut(s) 1008
Csp6I GTAC 6 cut(s) 115, 153, 247, 368, 517, 963
CviAII CATG 6 cut(s) 103, 239, 365, 431, 897, 1079
CviQI GTAC 6 cut(s) 115, 153, 247, 368, 517, 963
DdeI CTNAG 5 cut(s) 378, 468, 615, 789, 1069
DpnI GATC 5 cut(s) 140, 455, 491, 1399, 1406
DpnII GATC 5 cut(s) 138, 453, 489, 1397, 1404
DrdI GACNNNNNNGTC 2 cut(s) 435, 1100
DseDI GACNNNNNNGTC 2 cut(s) 435, 1100
EaeI YGGCCR 2 cut(s) 70, 1178
Eam1104I CTCTTC 4 cut(s) 506, 543, 734, 1276
EarI CTCTTC 4 cut(s) 506, 543, 734, 1276
Eco130I CCWWGG 2 cut(s) 102, 250
Eco47I GGWCC 1 cut(s) 119
Eco57I CTGAAG 3 cut(s) 1209, 1224, 1413
Eco88I CYCGRG 1 cut(s) 822
Eco91I GGTNACC 1 cut(s) 50
EcoO109I RGGNCCY 1 cut(s) 7
EcoO65I GGTNACC 1 cut(s) 50
EcoRII CCWGG 3 cut(s) 156, 1008, 1291
EcoT14I CCWWGG 2 cut(s) 102, 250
ErhI CCWWGG 2 cut(s) 102, 250
FaeI CATG 6 cut(s) 106, 242, 368, 434, 900, 1082
FalI AAGNNNNNCTT 2 cut(s) 503, 535
FatI CATG 6 cut(s) 102, 238, 364, 430, 896, 1078
FauNDI CATATG 1 cut(s) 134
FbaI TGATCA 2 cut(s) 453, 489
FblI GTMKAC 1 cut(s) 1233
Fnu4HI GCNGC 3 cut(s) 267, 761, 1108
FokI GGATG 3 cut(s) 652, 845, 1103
Fsp4HI GCNGC 3 cut(s) 267, 761, 1108
FspBI CTAG 6 cut(s) 65, 350, 495, 542, 863, 941
GluI GCNGC 3 cut(s) 267, 761, 1108
GsaI CCCAGC 1 cut(s) 510
HaeIII GGCC 3 cut(s) 9, 72, 1180
HapII CCGG 1 cut(s) 867
HgaI GACGC 3 cut(s) 1034, 1244, 1336
Hin1II CATG 6 cut(s) 106, 242, 368, 434, 900, 1082
HincII GTYRAC 1 cut(s) 1247
HindII GTYRAC 1 cut(s) 1247
HindIII AAGCTT 2 cut(s) 569, 1154
HinfI GANTC 8 cut(s) 355, 692, 746, 793, 853, 875, 1144, 1342
HpaII CCGG 1 cut(s) 867
HphI GGTGA 3 cut(s) 44, 273, 463
Hpy166II GTNNAC 4 cut(s) 465, 1015, 1234, 1247
Hpy188I TCNGA 6 cut(s) 89, 360, 379, 790, 874, 1462
Hpy188III TCNNGA 2 cut(s) 196, 797
Hpy8I GTNNAC 4 cut(s) 465, 1015, 1234, 1247
HpyAV CCTTC 1 cut(s) 1388
HpyCH4III ACNGT 7 cut(s) 84, 119, 152, 803, 904, 967, 1476
HpyCH4IV ACGT 2 cut(s) 486, 1450
HpyCH4V TGCA 5 cut(s) 236, 266, 736, 1098, 1307
HpyF10VI GCNNNNNNNGC 3 cut(s) 15, 1104, 1227
HpyF3I CTNAG 5 cut(s) 378, 468, 615, 789, 1069
HpySE526I ACGT 2 cut(s) 486, 1450
Hsp92II CATG 6 cut(s) 106, 242, 368, 434, 900, 1082
Ksp22I TGATCA 2 cut(s) 453, 489
Kzo9I GATC 5 cut(s) 138, 453, 489, 1397, 1404
LmnI GCTCC 2 cut(s) 681, 1066
Lsp1109I GCAGC 2 cut(s) 278, 772
LweI GCATC 1 cut(s) 723
MabI ACCWGGT 1 cut(s) 1008
MaeI CTAG 6 cut(s) 65, 350, 495, 542, 863, 941
MaeII ACGT 2 cut(s) 486, 1450
MaeIII GTNAC 4 cut(s) 50, 254, 610, 1297
MalI GATC 5 cut(s) 140, 455, 491, 1399, 1406
MbiI CCGCTC 1 cut(s) 1066
MboI GATC 5 cut(s) 138, 453, 489, 1397, 1404
MboII GAAGA 6 cut(s) 523, 560, 728, 751, 1001, 1293
MfeI CAATTG 1 cut(s) 656
MhlI GDGCHC 4 cut(s) 20, 37, 474, 1130
MlsI TGGCCA 1 cut(s) 1180
MluCI AATT 7 cut(s) 42, 621, 656, 686, 953, 1355, 1463
MluNI TGGCCA 1 cut(s) 1180
MlyI GAGTC 3 cut(s) 349, 686, 740
MnlI CCTC 8 cut(s) 65, 373, 507, 544, 552, 610, 943, 1084
Mox20I TGGCCA 1 cut(s) 1180
MroXI GAANNNNTTC 1 cut(s) 1209
MscI TGGCCA 1 cut(s) 1180
MseI TTAA 5 cut(s) 396, 917, 956, 1002, 1442
Msp20I TGGCCA 1 cut(s) 1180
MspI CCGG 1 cut(s) 867
MspR9I CCNGG 3 cut(s) 158, 1010, 1293
MunI CAATTG 1 cut(s) 656
Mva1269I GAATGC 1 cut(s) 269
MvaI CCWGG 3 cut(s) 158, 1010, 1293
MwoI GCNNNNNNNGC 3 cut(s) 15, 1104, 1227
NcoI CCATGG 1 cut(s) 102
NdeI CATATG 1 cut(s) 134
NdeII GATC 5 cut(s) 138, 453, 489, 1397, 1404
NheI GCTAGC 1 cut(s) 940
NlaIII CATG 6 cut(s) 106, 242, 368, 434, 900, 1082
NlaIV GGNNCC 3 cut(s) 34, 683, 1166
NmuCI GTSAC 2 cut(s) 50, 1297
NspI RCATGY 1 cut(s) 368
PciI ACATGT 1 cut(s) 364
PctI GAATGC 1 cut(s) 269
PdmI GAANNNNTTC 1 cut(s) 1209
PfeI GAWTC 5 cut(s) 793, 853, 875, 1144, 1342
PkrI GCNGC 3 cut(s) 268, 762, 1109
PleI GAGTC 3 cut(s) 349, 686, 740
PpsI GAGTC 3 cut(s) 349, 686, 740
PscI ACATGT 1 cut(s) 364
Psp6I CCWGG 3 cut(s) 156, 1008, 1291
PspEI GGTNACC 1 cut(s) 50
PspFI CCCAGC 1 cut(s) 506
PspGI CCWGG 3 cut(s) 156, 1008, 1291
PspN4I GGNNCC 3 cut(s) 34, 683, 1166
PspPI GGNCC 2 cut(s) 7, 119
PstNI CAGNNNCTG 1 cut(s) 1187
RsaI GTAC 6 cut(s) 116, 154, 248, 369, 518, 964
RsaNI GTAC 6 cut(s) 115, 153, 247, 368, 517, 963
SaqAI TTAA 5 cut(s) 396, 917, 956, 1002, 1442
SatI GCNGC 3 cut(s) 267, 761, 1108
Sau3AI GATC 5 cut(s) 138, 453, 489, 1397, 1404
Sau96I GGNCC 2 cut(s) 7, 119
SchI GAGTC 3 cut(s) 349, 686, 740
ScrFI CCNGG 3 cut(s) 158, 1010, 1293
SduI GDGCHC 4 cut(s) 20, 37, 474, 1130
SexAI ACCWGGT 1 cut(s) 1008
SfaNI GCATC 1 cut(s) 723
SinI GGWCC 1 cut(s) 119
SmlI CTYRAG 1 cut(s) 748
SmoI CTYRAG 1 cut(s) 748
Sse9I AATT 7 cut(s) 42, 621, 656, 686, 953, 1355, 1463
SsiI CCGC 2 cut(s) 1064, 1107
SspMI CTAG 6 cut(s) 65, 350, 495, 542, 863, 941
StyD4I CCNGG 3 cut(s) 156, 1008, 1291
StyI CCWWGG 2 cut(s) 102, 250
TaaI ACNGT 7 cut(s) 84, 119, 152, 803, 904, 967, 1476
TaiI ACGT 2 cut(s) 489, 1453
TasI AATT 7 cut(s) 42, 621, 656, 686, 953, 1355, 1463
TatI WGTACW 1 cut(s) 367
TauI GCSGC 1 cut(s) 1110
TfiI GAWTC 5 cut(s) 793, 853, 875, 1144, 1342
Tru1I TTAA 5 cut(s) 396, 917, 956, 1002, 1442
Tru9I TTAA 5 cut(s) 396, 917, 956, 1002, 1442
TscAI CASTG 6 cut(s) 87, 463, 855, 1047, 1386, 1434
TseFI GTSAC 2 cut(s) 50, 1297
TseI GCWGC 2 cut(s) 266, 760
Tsp45I GTSAC 2 cut(s) 50, 1297
TspDTI ATGAA 8 cut(s) 17, 189, 552, 561, 760, 1095, 1148, 1249
TspRI CASTG 6 cut(s) 87, 463, 855, 1047, 1386, 1434
VpaK11BI GGWCC 1 cut(s) 119
XceI RCATGY 1 cut(s) 368
XcmI CCANNNNNNNNNTGG 1 cut(s) 65
XmiI GTMKAC 1 cut(s) 1233
XmnI GAANNNNTTC 1 cut(s) 1209
XspI CTAG 6 cut(s) 65, 350, 495, 542, 863, 941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.