MD05G1169900.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006974
Physical Location & Seq
Forward (+)
29954733 .. 29957273
2541 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1169900.v1.1.491

Sequence Viewer

Length: 1575 bp
ATGGATTTTCCTTCTCATCTTCTGGCAATTGCAGGGATTTTACTCTTTGTTCTGTATCTATGGAGGGTAAAAATCCAAAGTCACAAAACCAAGGGCGTCTTGACCCCACAACCATCAGGTGCCCTGCCAATCATAGGACACCTCCACAAACTTGGAGGCAAAAACCCACTTTGCAGAACCCTAGCATCCATGGCTGACAAGTACGGTCCAATCTTCACCATACGGCTTGGCAAGCACCCTGCACTTGTGATCAGCAACCATGATGCAGTCAAGGAATGCTTCACTAAAAACGACATCGTTTTCGCAGCGAGACCAAAGTCGGCACATGGCAGGTACCTTGGTTACAATTATGCAGGTTTCGGGTTCTCACCATACGGCACGTACTGGCGTAACATGAGAAAAATGGTCATCGCTGAGTTGCTATCTAGCCGCAGGCTTCAGACATTAAAACATGTCCAAATCTCCGAGGTTGATTCTTTTATCAAGGGGTTGTACGAAATTTGCAAAACAAAGGGGGGAAATGGTCAAAGCAAGGTGGTGATTAGCGAGTGGATTGAGCACCTGACTTTGAATGTAATTACTCAAATGATTGCTGGGAAGAGGTATTTTGATTGGGGTAAAGTGGGAAATGACTCAGATAATGAGGGAGAGAATAAGAGGATTCGGGAAATCGTGAAGGAGTTTATGCTTGTGTCTGGGATGCCAGTCATTTCTGATATGATTGGGTATCCGGATTGGATTGATTTTCTGGGGCAAGTGAAGAATATGAAGCGTATAGGGAAGGAGTTGGATGCTCTAACGGCAAGTTGGATTGAAGAACATAATAATGCAAGGAAGGAGATCGACTCAAGGGACAAGCTCGACTTCATTGATGTCATGCTTTCTGTGATTGAGGACAATACTGTGCTTGGTCATACTCGTGAAACCATCATCAAGGCAACATCGCAGAATCTGATCATAGCTGGCTCAGATACCACAGCAATCAGCTTGAAGTGGATCATCTCTTTATTATTAAACAACAGGCATGTTTTGAAGCATGCACAAGAAGAGATAAACCTAAAAGTTGGCACAGAGAGATGGGTTGAAGACACTGATATCGAAAACCTAACTTACCTCCAAGCCATTGTCAAGGAAACCCTACGTCTATATCCTCCAGGTCCACTCTCAGTCCCACATGAAGCAATGGAAGACTGCCAAGTTTGTGGCTTTCACATTCCAAAGGGCACACGTTTATTTGTCAATTTGTGGAAATTGCACAGAGACCCAACTATGTGGTCAGACCCTGAAATGTTTTACCCAGATAGGTTTCTTACAACCCAAGCAAGCATTGATGCCTCTGGTAACCATTTTGAGTTCATTCCATTTGGGTCTGGAAGAAGATCTTGTCCAGGTCTCACATTCGCAATGCAAGTTATCCACTTGACTCTTGGTAGATTGATTCAAGGATTTGAATTGGCTACTCCATTGGACATGCCTGTAGACATGACTGAAGGACTAGGCGTTACTTTGCCAAAGGCAACTTCACTTGAAGTTGTGCTAACTCCACGCTTGCCCATTGAATTGTATGAAAGATAG

Protein Analysis

525

Amino Acids

59.19

Weight (kDa)

8.09

Isoelectric Point (pI)

36.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 36 - 505 1e-98 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 321, 344
Acc65I GGTACC 1 cut(s) 333
AccB1I GGYRCC 2 cut(s) 119, 333
AccI GTMKAC 1 cut(s) 1479
AccIII TCCGGA 1 cut(s) 730
AciI CCGC 1 cut(s) 430
AclWI GGATC 1 cut(s) 1004
AcsI RAATTY 1 cut(s) 498
AcuI CTGAAG 2 cut(s) 422, 1509
AcyI GRCGYC 1 cut(s) 96
AfaI GTAC 4 cut(s) 203, 335, 383, 494
AfiI CCNNNNNNNGG 1 cut(s) 134
AflIII ACRYGT 2 cut(s) 451, 1226
AgsI TTSAA 9 cut(s) 571, 815, 991, 1033, 1085, 1442, 1451, 1529, 1559
AjnI CCWGG 2 cut(s) 1153, 1387
AjuI GAANNNNNNNTTGG 2 cut(s) 1504, 1536
AluBI AGCT 3 cut(s) 859, 962, 987
AluI AGCT 3 cut(s) 859, 962, 987
Alw21I GWGCWC 1 cut(s) 561
Alw26I GTCTC 3 cut(s) 304, 1254, 1397
AlwI GGATC 1 cut(s) 1004
AlwNI CAGNNNCTG 2 cut(s) 952, 1283
Aor13HI TCCGGA 1 cut(s) 730
ApeKI GCWGC 1 cut(s) 305
ApoI RAATTY 1 cut(s) 498
ArsI GACNNNNNNTTYG 2 cut(s) 284, 316
Asp718I GGTACC 1 cut(s) 333
AspS9I GGNCC 2 cut(s) 206, 1157
AsuHPI GGTGA 3 cut(s) 208, 360, 550
AvaII GGWCC 2 cut(s) 206, 1157
BaeGI GKGCMC 2 cut(s) 124, 1226
BaeI ACNNNNGTAYC 2 cut(s) 325, 358
BanI GGYRCC 2 cut(s) 119, 333
BauI CACGAG 1 cut(s) 918
BbsI GAAGAC 2 cut(s) 1092, 1194
Bbv12I GWGCWC 1 cut(s) 561
BbvI GCAGC 1 cut(s) 317
BccI CCATC 3 cut(s) 121, 935, 1071
BceAI ACGGC 3 cut(s) 239, 391, 816
BciT130I CCWGG 2 cut(s) 1155, 1389
BciVI GTATCC 1 cut(s) 738
BclI TGATCA 2 cut(s) 249, 954
BcoDI GTCTC 3 cut(s) 304, 1254, 1397
BfaI CTAG 3 cut(s) 182, 426, 1496
BfmI CTRYAG 1 cut(s) 1476
BfuAI ACCTGC 2 cut(s) 321, 344
BfuI GTATCC 1 cut(s) 738
BglII AGATCT 1 cut(s) 1379
BisI GCNGC 2 cut(s) 306, 430
BlsI GCNGC 2 cut(s) 307, 431
Bme1390I CCNGG 2 cut(s) 1155, 1389
Bme18I GGWCC 2 cut(s) 206, 1157
BmgT120I GGNCC 2 cut(s) 206, 1157
BmiI GGNNCC 2 cut(s) 121, 335
BmrFI CCNGG 2 cut(s) 1155, 1389
BmsI GCATC 5 cut(s) 194, 253, 690, 781, 1321
BoxI GACNNNNGTC 1 cut(s) 316
BpiI GAAGAC 2 cut(s) 1092, 1194
BplI GAGNNNNNCTC 2 cut(s) 830, 862
BpmI CTGGAG 1 cut(s) 1137
BpuEI CTTGAG 1 cut(s) 832
BsaAI YACGTR 1 cut(s) 381
BsaHI GRCGYC 1 cut(s) 96
BsaI GGTCTC 3 cut(s) 304, 1254, 1397
BsaJI CCNNGG 4 cut(s) 90, 189, 337, 465
BsaWI WCCGGW 1 cut(s) 730
Bsc4I CCNNNNNNNGG 1 cut(s) 134
Bse1I ACTGG 2 cut(s) 389, 704
Bse3DI GCAATG 2 cut(s) 1188, 1410
BseAI TCCGGA 1 cut(s) 730
BseBI CCWGG 2 cut(s) 1155, 1389
BseDI CCNNGG 4 cut(s) 90, 189, 337, 465
BseGI GGATG 3 cut(s) 185, 705, 796
BseLI CCNNNNNNNGG 1 cut(s) 134
BseMI GCAATG 2 cut(s) 1188, 1410
BseMII CTCAG 4 cut(s) 405, 648, 981, 1179
BseNI ACTGG 2 cut(s) 389, 704
BseSI GKGCMC 2 cut(s) 124, 1226
BseXI GCAGC 1 cut(s) 317
BseYI CCCAGC 1 cut(s) 593
BsgI GTGCAG 1 cut(s) 225
BshNI GGYRCC 2 cut(s) 119, 333
BsiHKAI GWGCWC 1 cut(s) 561
BsiSI CCGG 1 cut(s) 731
BslFI GGGAC 2 cut(s) 866, 1154
BslI CCNNNNNNNGG 1 cut(s) 134
BsmAI GTCTC 3 cut(s) 304, 1254, 1397
BsmFI GGGAC 2 cut(s) 866, 1154
BsmI GAATGC 1 cut(s) 281
Bso31I GGTCTC 3 cut(s) 304, 1254, 1397
Bsp1286I GDGCHC 3 cut(s) 124, 561, 1226
Bsp13I TCCGGA 1 cut(s) 730
Bsp143I GATC 5 cut(s) 249, 840, 954, 996, 1379
Bsp19I CCATGG 1 cut(s) 189
BspACI CCGC 1 cut(s) 430
BspCNI CTCAG 4 cut(s) 406, 647, 980, 1178
BspEI TCCGGA 1 cut(s) 730
BspLI GGNNCC 2 cut(s) 121, 335
BspMI ACCTGC 2 cut(s) 321, 344
BspPI GGATC 1 cut(s) 1004
BspT107I GGYRCC 2 cut(s) 119, 333
BspTNI GGTCTC 3 cut(s) 304, 1254, 1397
BsrDI GCAATG 2 cut(s) 1188, 1410
BsrI ACTGG 2 cut(s) 389, 704
BssECI CCNNGG 4 cut(s) 90, 189, 337, 465
BssMI GATC 5 cut(s) 249, 840, 954, 996, 1379
BssNI GRCGYC 1 cut(s) 96
BssSI CACGAG 1 cut(s) 918
BssT1I CCWWGG 3 cut(s) 90, 189, 337
Bst2BI CACGAG 1 cut(s) 918
Bst2UI CCWGG 2 cut(s) 1155, 1389
Bst4CI ACNGT 2 cut(s) 206, 904
Bst6I CTCTTC 2 cut(s) 593, 1041
BstACI GRCGYC 1 cut(s) 96
BstBAI YACGTR 1 cut(s) 381
BstC8I GCNNGC 6 cut(s) 233, 434, 964, 1038, 1324, 1550
BstDEI CTNAG 4 cut(s) 414, 634, 967, 1165
BstDSI CCRYGG 1 cut(s) 189
BstEII GGTNACC 1 cut(s) 1340
BstF5I GGATG 3 cut(s) 185, 705, 796
BstKTI GATC 5 cut(s) 252, 843, 957, 999, 1382
BstMAI GTCTC 3 cut(s) 304, 1254, 1397
BstMBI GATC 5 cut(s) 249, 840, 954, 996, 1379
BstMWI GCNNNNNNNGC 3 cut(s) 191, 232, 800
BstNI CCWGG 2 cut(s) 1155, 1389
BstNSI RCATGY 4 cut(s) 455, 1028, 1040, 1474
BstPAI GACNNNNGTC 1 cut(s) 316
BstPI GGTNACC 1 cut(s) 1340
BstSCI CCNGG 2 cut(s) 1153, 1387
BstSFI CTRYAG 1 cut(s) 1476
BstSLI GKGCMC 2 cut(s) 124, 1226
BstV1I GCAGC 1 cut(s) 317
BstV2I GAAGAC 2 cut(s) 1092, 1194
BstX2I RGATCY 1 cut(s) 1379
BstXI CCANNNNNNTGG 3 cut(s) 152, 1202, 1272
BstYI RGATCY 1 cut(s) 1379
BsuI GTATCC 1 cut(s) 738
BtgI CCRYGG 1 cut(s) 189
BtgZI GCGATG 2 cut(s) 394, 927
BtsCI GGATG 3 cut(s) 185, 705, 796
BtsIMutI CAGTG 1 cut(s) 1089
BveI ACCTGC 2 cut(s) 321, 344
Cac8I GCNNGC 6 cut(s) 233, 434, 964, 1038, 1324, 1550
CaiI CAGNNNCTG 2 cut(s) 952, 1283
Cfr13I GGNCC 2 cut(s) 206, 1157
CseI GACGC 1 cut(s) 85
Csp6I GTAC 4 cut(s) 202, 334, 382, 493
CviQI GTAC 4 cut(s) 202, 334, 382, 493
DdeI CTNAG 4 cut(s) 414, 634, 967, 1165
DpnI GATC 5 cut(s) 251, 842, 956, 998, 1381
DpnII GATC 5 cut(s) 249, 840, 954, 996, 1379
Eam1104I CTCTTC 2 cut(s) 593, 1041
EarI CTCTTC 2 cut(s) 593, 1041
Eco130I CCWWGG 3 cut(s) 90, 189, 337
Eco31I GGTCTC 3 cut(s) 304, 1254, 1397
Eco32I GATATC 1 cut(s) 1096
Eco47I GGWCC 2 cut(s) 206, 1157
Eco57I CTGAAG 2 cut(s) 422, 1509
Eco91I GGTNACC 1 cut(s) 1340
EcoO65I GGTNACC 1 cut(s) 1340
EcoRII CCWGG 2 cut(s) 1153, 1387
EcoRV GATATC 1 cut(s) 1096
EcoT14I CCWWGG 3 cut(s) 90, 189, 337
ErhI CCWWGG 3 cut(s) 90, 189, 337
FalI AAGNNNNNCTT 8 cut(s) 83, 115, 263, 295, 848, 880, 1366, 1398
FaqI GGGAC 2 cut(s) 866, 1154
FbaI TGATCA 2 cut(s) 249, 954
FblI GTMKAC 1 cut(s) 1479
Fnu4HI GCNGC 2 cut(s) 306, 430
FokI GGATG 3 cut(s) 172, 712, 803
Fsp4HI GCNGC 2 cut(s) 306, 430
FspBI CTAG 3 cut(s) 182, 426, 1496
GluI GCNGC 2 cut(s) 306, 430
GsaI CCCAGC 1 cut(s) 597
GsuI CTGGAG 1 cut(s) 1137
HapII CCGG 1 cut(s) 731
HgaI GACGC 1 cut(s) 85
Hin1I GRCGYC 1 cut(s) 96
HinfI GANTC 7 cut(s) 473, 632, 661, 845, 949, 1423, 1438
HpaII CCGG 1 cut(s) 731
HphI GGTGA 3 cut(s) 208, 360, 550
Hpy166II GTNNAC 2 cut(s) 1160, 1480
Hpy188I TCNGA 7 cut(s) 441, 466, 637, 715, 954, 970, 1279
Hpy188III TCNNGA 6 cut(s) 100, 665, 673, 731, 920, 1371
Hpy8I GTNNAC 2 cut(s) 1160, 1480
HpyAV CCTTC 5 cut(s) 21, 670, 775, 829, 1484
HpyCH4III ACNGT 2 cut(s) 206, 904
HpyCH4IV ACGT 3 cut(s) 380, 1141, 1228
HpyF10VI GCNNNNNNNGC 3 cut(s) 191, 232, 800
HpyF3I CTNAG 4 cut(s) 414, 634, 967, 1165
HpySE526I ACGT 3 cut(s) 380, 1141, 1228
Hsp92I GRCGYC 1 cut(s) 96
Kpn2I TCCGGA 1 cut(s) 730
KpnI GGTACC 1 cut(s) 337
Ksp22I TGATCA 2 cut(s) 249, 954
Kzo9I GATC 5 cut(s) 249, 840, 954, 996, 1379
Lsp1109I GCAGC 1 cut(s) 317
LweI GCATC 5 cut(s) 194, 253, 690, 781, 1321
MaeI CTAG 3 cut(s) 182, 426, 1496
MaeII ACGT 3 cut(s) 380, 1141, 1228
MaeIII GTNAC 5 cut(s) 80, 341, 389, 1340, 1501
MalI GATC 5 cut(s) 251, 842, 956, 998, 1381
MboI GATC 5 cut(s) 249, 840, 954, 996, 1379
MfeI CAATTG 1 cut(s) 27
MflI RGATCY 1 cut(s) 1379
MhlI GDGCHC 3 cut(s) 124, 561, 1226
MluCI AATT 8 cut(s) 27, 346, 498, 576, 1240, 1250, 1451, 1559
MlyI GAGTC 3 cut(s) 626, 839, 1417
MmeI TCCRAC 2 cut(s) 768, 788
MroI TCCGGA 1 cut(s) 730
MseI TTAA 2 cut(s) 446, 1013
MslI CAYNNNNRTG 2 cut(s) 825, 918
MspI CCGG 1 cut(s) 731
MspR9I CCNGG 2 cut(s) 1155, 1389
MunI CAATTG 1 cut(s) 27
Mva1269I GAATGC 1 cut(s) 281
MvaI CCWGG 2 cut(s) 1155, 1389
MwoI GCNNNNNNNGC 3 cut(s) 191, 232, 800
NcoI CCATGG 1 cut(s) 189
NdeII GATC 5 cut(s) 249, 840, 954, 996, 1379
NlaIV GGNNCC 2 cut(s) 121, 335
NmuCI GTSAC 1 cut(s) 80
NspI RCATGY 4 cut(s) 455, 1028, 1040, 1474
PaeI GCATGC 1 cut(s) 1040
PciI ACATGT 1 cut(s) 451
PctI GAATGC 1 cut(s) 281
PfeI GAWTC 4 cut(s) 473, 661, 949, 1438
PkrI GCNGC 2 cut(s) 307, 431
PleI GAGTC 3 cut(s) 626, 839, 1417
PpsI GAGTC 3 cut(s) 626, 839, 1417
Ppu21I YACGTR 1 cut(s) 381
PscI ACATGT 1 cut(s) 451
PshAI GACNNNNGTC 1 cut(s) 316
Psp6I CCWGG 2 cut(s) 1153, 1387
PspEI GGTNACC 1 cut(s) 1340
PspFI CCCAGC 1 cut(s) 593
PspGI CCWGG 2 cut(s) 1153, 1387
PspN4I GGNNCC 2 cut(s) 121, 335
PspPI GGNCC 2 cut(s) 206, 1157
PstNI CAGNNNCTG 2 cut(s) 952, 1283
PsuI RGATCY 1 cut(s) 1379
RsaI GTAC 4 cut(s) 203, 335, 383, 494
RsaNI GTAC 4 cut(s) 202, 334, 382, 493
RseI CAYNNNNRTG 2 cut(s) 825, 918
SaqAI TTAA 2 cut(s) 446, 1013
SatI GCNGC 2 cut(s) 306, 430
Sau3AI GATC 5 cut(s) 249, 840, 954, 996, 1379
Sau96I GGNCC 2 cut(s) 206, 1157
SchI GAGTC 3 cut(s) 626, 839, 1417
ScrFI CCNGG 2 cut(s) 1155, 1389
SduI GDGCHC 3 cut(s) 124, 561, 1226
SfaNI GCATC 5 cut(s) 194, 253, 690, 781, 1321
SfcI CTRYAG 1 cut(s) 1476
SinI GGWCC 2 cut(s) 206, 1157
SmiMI CAYNNNNRTG 2 cut(s) 825, 918
SmlI CTYRAG 1 cut(s) 847
SmoI CTYRAG 1 cut(s) 847
SphI GCATGC 1 cut(s) 1040
Sse9I AATT 8 cut(s) 27, 346, 498, 576, 1240, 1250, 1451, 1559
SsiI CCGC 1 cut(s) 430
SspMI CTAG 3 cut(s) 182, 426, 1496
StyD4I CCNGG 2 cut(s) 1153, 1387
StyI CCWWGG 3 cut(s) 90, 189, 337
TaaI ACNGT 2 cut(s) 206, 904
TaiI ACGT 3 cut(s) 383, 1144, 1231
TaqI TCGA 3 cut(s) 843, 861, 1098
TasI AATT 8 cut(s) 27, 346, 498, 576, 1240, 1250, 1451, 1559
TauI GCSGC 1 cut(s) 432
TfiI GAWTC 4 cut(s) 473, 661, 949, 1438
Tru1I TTAA 2 cut(s) 446, 1013
Tru9I TTAA 2 cut(s) 446, 1013
TscAI CASTG 1 cut(s) 1096
TseFI GTSAC 1 cut(s) 80
TseI GCWGC 1 cut(s) 305
Tsp45I GTSAC 1 cut(s) 80
TspDTI ATGAA 4 cut(s) 782, 856, 1191, 1345
TspRI CASTG 1 cut(s) 1096
VpaK11BI GGWCC 2 cut(s) 206, 1157
XapI RAATTY 1 cut(s) 498
XceI RCATGY 4 cut(s) 455, 1028, 1040, 1474
XcmI CCANNNNNNNNNTGG 1 cut(s) 1424
XmiI GTMKAC 1 cut(s) 1479
XspI CTAG 3 cut(s) 182, 426, 1496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.