RchiOBHm_Chr6g0283841

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0006974
Physical Location & Seq
Forward (+)
47070903 .. 47073044
2142 bp
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UTR
Exon/CDS
Intron
PRQ25452

Sequence Viewer

Length: 1377 bp
ATGGCTGACAAGTATGGTCCAATCTTTACCATATGGCTCGGCAAGAAGCGTGCCCTGGTGATCAACAACTACGAAGCTGTCAAGGAATGTTTCACCACAAACGACAGGATTTTCGCCACAAGACCAATTTCTGCACAGGGAGAGTACCTTGGTTACAATTATGCAGCATTCGGGTTCTCACCCTATGGCACATATTGGCGTGATATGAGAAAGTTGGTTGTGATCGAGTTGCTCTCTAATCGTAGGCTAGAGACTCTGAAACATGTACAAGTCTCTGAGGTTGATGCTTTTGTTAAAGGGTTGTATTCAGTTTGGGAAAACAAAGGACGTGATGGTCAAAGCAAGGTGGTGATCAGTGAATGGATTGAGCACCTAACCTTAAACGTGATCACTAGAATTATTGCTGGGAAGAGGTACTTTGGTAAAGAGAGTGATGAACTTGATGGAGAGCAAAAGAGGATTGGGAAGCTTATGAAGGACTTTATGTATGCTGCTGGCAAACCAGTTGTTTCTGATGTAATTGGGTTTCCAAGGTGGATGGATTTCAAGGGGCAATTGAAAACTATGAAGAATATAGGGAGCCAATTAGACTCTTTGATGACAAGTTGGGTTGAAGAACATCATACAAAGAAGCAGAAGAGTGACTCAAGCAACCAGCAGCAGGACTTCATTAATGTCCTGCTTTCTGAGATTGAGGACAGTTCTATGCTTGATCATACCCGAGATACTATTATCAAGGCAACAGCACTGAATCTCATCCTAGCCGGTTCAGAATCCACATCAATCAGCTTGACATGGACCGTCTCTTTACTATTAAACAACAGACATATCTTGAAGGTAGCCCAAGAGGAATTAGATCGAACAGTTGGCAGGCACAGATGGGTGGAAGACACTGACATTAAAAACCTGGTTTACCTCCAAGCCACCGTCAAAGAAACATTGCGTCTATACCCACCAGCTCCACTCTCAGTTCCACATGAAGCAATGGAGGACTGCAAAGTCGGCGGTTTTGACATCCCGAAGGGCACTCTTTTGTTTGTGAATCTGTGGAAGCTTCATAGGAACCCAAGTGTGTGGTCAGACCCTGAAGTGTTTTCCCCAGAAAGATTTCTTACAAGCCAAGCAGGTGTAGACGCTTCAGGTCAACATTTTGAGTTCATTCCTTTTGGGTCTGGGAGAAGAGTTTGCCCAGGTATCACTTTTGCGTTTCAGCTTATACACTTGACGCTGGCAAGGTTGATTCAAGGGTTTGAATTGGCAACTCCATTGGATAAGGCAGTGGATATGACTGAAGGATTGGGGATCACTATACCAAGAGCAACTCCACTGGAAGTTCTCTTGACCCCACGTTTAGCTTTTGAGTTGTATCCACAGTAA

Protein Analysis

458

Amino Acids

52.0

Weight (kDa)

6.85

Isoelectric Point (pI)

37.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 2 - 438 2.9e-94 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1115
AasI GACNNNNNNGTC 2 cut(s) 333, 998
Acc36I ACCTGC 1 cut(s) 1115
AccI GTMKAC 1 cut(s) 1131
AciI CCGC 1 cut(s) 1005
AclWI GGATC 1 cut(s) 1310
AcuI CTGAAG 3 cut(s) 1107, 1122, 1311
AfaI GTAC 3 cut(s) 146, 267, 416
AfiI CCNNNNNNNGG 1 cut(s) 661
AflIII ACRYGT 1 cut(s) 262
AgsI TTSAA 6 cut(s) 547, 559, 614, 835, 1244, 1253
AjiI CACGTC 1 cut(s) 329
AjnI CCWGG 3 cut(s) 54, 906, 1189
AluBI AGCT 7 cut(s) 77, 469, 789, 959, 1054, 1213, 1355
AluI AGCT 7 cut(s) 77, 469, 789, 959, 1054, 1213, 1355
Alw21I GWGCWC 1 cut(s) 372
Alw26I GTCTC 3 cut(s) 245, 277, 808
AlwI GGATC 1 cut(s) 1310
AlwNI CAGNNNCTG 1 cut(s) 1085
Ama87I CYCGRG 1 cut(s) 720
ApeKI GCWGC 3 cut(s) 164, 491, 658
ArsI GACNNNNNNTTYG 2 cut(s) 95, 127
AseI ATTAAT 1 cut(s) 672
Asp700I GAANNNNTTC 1 cut(s) 1107
AspS9I GGNCC 2 cut(s) 17, 798
AsuHPI GGTGA 4 cut(s) 70, 85, 171, 361
AvaI CYCGRG 1 cut(s) 720
AvaII GGWCC 2 cut(s) 17, 798
BaeGI GKGCMC 2 cut(s) 55, 1028
BaeI ACNNNNGTAYC 2 cut(s) 406, 439
BbsI GAAGAC 1 cut(s) 894
Bbv12I GWGCWC 1 cut(s) 372
BbvI GCAGC 3 cut(s) 176, 478, 670
BccI CCATC 4 cut(s) 326, 437, 532, 873
BciT130I CCWGG 3 cut(s) 56, 908, 1191
BciVI GTATCC 1 cut(s) 1377
BclI TGATCA 4 cut(s) 60, 351, 387, 712
BcoDI GTCTC 3 cut(s) 245, 277, 808
BfaI CTAG 3 cut(s) 248, 393, 761
BfuAI ACCTGC 1 cut(s) 1115
BfuI GTATCC 1 cut(s) 1377
BisI GCNGC 3 cut(s) 165, 492, 659
BlsI GCNGC 3 cut(s) 166, 493, 660
Bme1390I CCNGG 3 cut(s) 56, 908, 1191
Bme18I GGWCC 2 cut(s) 17, 798
BmeT110I CYCGRG 1 cut(s) 720
BmgBI CACGTC 1 cut(s) 329
BmgT120I GGNCC 2 cut(s) 17, 798
BmiI GGNNCC 2 cut(s) 581, 1064
BmrFI CCNGG 3 cut(s) 56, 908, 1191
BmsI GCATC 1 cut(s) 274
BpiI GAAGAC 1 cut(s) 894
BplI GAGNNNNNCTC 2 cut(s) 218, 250
BpuEI CTTGAG 1 cut(s) 631
BsaJI CCNNGG 4 cut(s) 54, 148, 530, 1189
Bsc4I CCNNNNNNNGG 1 cut(s) 661
Bse118I RCCGGY 1 cut(s) 764
Bse1I ACTGG 2 cut(s) 503, 1332
Bse3DI GCAATG 2 cut(s) 938, 990
BseBI CCWGG 3 cut(s) 56, 908, 1191
BseDI CCNNGG 4 cut(s) 54, 148, 530, 1189
BseGI GGATG 3 cut(s) 543, 756, 1014
BseLI CCNNNNNNNGG 1 cut(s) 661
BseMI GCAATG 2 cut(s) 938, 990
BseMII CTCAG 3 cut(s) 267, 678, 981
BseNI ACTGG 2 cut(s) 503, 1332
BseSI GKGCMC 2 cut(s) 55, 1028
BseXI GCAGC 3 cut(s) 176, 478, 670
BseYI CCCAGC 1 cut(s) 404
BsgI GTGCAG 1 cut(s) 117
BsiHKAI GWGCWC 1 cut(s) 372
BsiHKCI CYCGRG 1 cut(s) 720
BsiSI CCGG 1 cut(s) 765
BslI CCNNNNNNNGG 1 cut(s) 661
BsmAI GTCTC 3 cut(s) 245, 277, 808
BsmBI CGTCTC 1 cut(s) 808
BsmI GAATGC 1 cut(s) 167
BsoBI CYCGRG 1 cut(s) 720
Bsp1286I GDGCHC 3 cut(s) 55, 372, 1028
Bsp1407I TGTACA 1 cut(s) 265
Bsp143I GATC 7 cut(s) 60, 222, 351, 387, 712, 856, 1302
BspACI CCGC 1 cut(s) 1005
BspCNI CTCAG 3 cut(s) 268, 679, 980
BspLI GGNNCC 2 cut(s) 581, 1064
BspMI ACCTGC 1 cut(s) 1115
BspPI GGATC 1 cut(s) 1310
BsrDI GCAATG 2 cut(s) 938, 990
BsrFI RCCGGY 1 cut(s) 764
BsrGI TGTACA 1 cut(s) 265
BsrI ACTGG 2 cut(s) 503, 1332
BssAI RCCGGY 1 cut(s) 764
BssECI CCNNGG 4 cut(s) 54, 148, 530, 1189
BssMI GATC 7 cut(s) 60, 222, 351, 387, 712, 856, 1302
BssT1I CCWWGG 2 cut(s) 148, 530
Bst2UI CCWGG 3 cut(s) 56, 908, 1191
Bst4CI ACNGT 5 cut(s) 701, 802, 865, 928, 1374
Bst6I CTCTTC 3 cut(s) 404, 632, 1174
BstAUI TGTACA 1 cut(s) 265
BstC8I GCNNGC 4 cut(s) 51, 496, 872, 1230
BstDEI CTNAG 3 cut(s) 276, 687, 967
BstF5I GGATG 3 cut(s) 543, 756, 1014
BstKTI GATC 7 cut(s) 63, 225, 354, 390, 715, 859, 1305
BstMAI GTCTC 3 cut(s) 245, 277, 808
BstMBI GATC 7 cut(s) 60, 222, 351, 387, 712, 856, 1302
BstMWI GCNNNNNNNGC 1 cut(s) 1002
BstNI CCWGG 3 cut(s) 56, 908, 1191
BstNSI RCATGY 1 cut(s) 266
BstSCI CCNGG 3 cut(s) 54, 906, 1189
BstSLI GKGCMC 2 cut(s) 55, 1028
BstV1I GCAGC 3 cut(s) 176, 478, 670
BstV2I GAAGAC 1 cut(s) 894
BstXI CCANNNNNNTGG 1 cut(s) 1074
BsuI GTATCC 1 cut(s) 1377
BtrI CACGTC 1 cut(s) 329
BtsCI GGATG 3 cut(s) 543, 756, 1014
BtsI GCAGTG 1 cut(s) 1284
BtsIMutI CAGTG 5 cut(s) 361, 746, 891, 1284, 1325
BveI ACCTGC 1 cut(s) 1115
Cac8I GCNNGC 4 cut(s) 51, 496, 872, 1230
CaiI CAGNNNCTG 1 cut(s) 1085
Cfr10I RCCGGY 1 cut(s) 764
Cfr13I GGNCC 2 cut(s) 17, 798
CseI GACGC 3 cut(s) 932, 1142, 1234
CsiI ACCWGGT 1 cut(s) 906
Csp6I GTAC 3 cut(s) 145, 266, 415
CviAII CATG 3 cut(s) 263, 795, 977
CviQI GTAC 3 cut(s) 145, 266, 415
DdeI CTNAG 3 cut(s) 276, 687, 967
DpnI GATC 7 cut(s) 62, 224, 353, 389, 714, 858, 1304
DpnII GATC 7 cut(s) 60, 222, 351, 387, 712, 856, 1302
DrdI GACNNNNNNGTC 2 cut(s) 333, 998
DseDI GACNNNNNNGTC 2 cut(s) 333, 998
Eam1104I CTCTTC 3 cut(s) 404, 632, 1174
EarI CTCTTC 3 cut(s) 404, 632, 1174
Eco130I CCWWGG 2 cut(s) 148, 530
Eco47I GGWCC 2 cut(s) 17, 798
Eco57I CTGAAG 3 cut(s) 1107, 1122, 1311
Eco88I CYCGRG 1 cut(s) 720
EcoRII CCWGG 3 cut(s) 54, 906, 1189
EcoT14I CCWWGG 2 cut(s) 148, 530
ErhI CCWWGG 2 cut(s) 148, 530
Esp3I CGTCTC 1 cut(s) 808
FaeI CATG 3 cut(s) 266, 798, 980
FalI AAGNNNNNCTT 2 cut(s) 401, 433
FatI CATG 3 cut(s) 262, 794, 976
FauNDI CATATG 1 cut(s) 32
FbaI TGATCA 4 cut(s) 60, 351, 387, 712
FblI GTMKAC 1 cut(s) 1131
Fnu4HI GCNGC 3 cut(s) 165, 492, 659
FokI GGATG 3 cut(s) 550, 743, 1001
Fsp4HI GCNGC 3 cut(s) 165, 492, 659
FspBI CTAG 3 cut(s) 248, 393, 761
GluI GCNGC 3 cut(s) 165, 492, 659
GsaI CCCAGC 1 cut(s) 408
HapII CCGG 1 cut(s) 765
HgaI GACGC 3 cut(s) 932, 1142, 1234
Hin1II CATG 3 cut(s) 266, 798, 980
HincII GTYRAC 1 cut(s) 1145
HindII GTYRAC 1 cut(s) 1145
HindIII AAGCTT 2 cut(s) 467, 1052
HinfI GANTC 7 cut(s) 253, 590, 644, 751, 773, 1042, 1240
HpaII CCGG 1 cut(s) 765
HphI GGTGA 4 cut(s) 70, 85, 171, 361
Hpy166II GTNNAC 3 cut(s) 913, 1132, 1145
Hpy188I TCNGA 6 cut(s) 258, 277, 514, 688, 772, 1081
Hpy188III TCNNGA 3 cut(s) 832, 1018, 1339
Hpy8I GTNNAC 3 cut(s) 913, 1132, 1145
HpyAV CCTTC 4 cut(s) 469, 829, 1015, 1286
HpyCH4III ACNGT 5 cut(s) 701, 802, 865, 928, 1374
HpyCH4IV ACGT 3 cut(s) 328, 384, 1348
HpyCH4V TGCA 3 cut(s) 134, 164, 996
HpyF10VI GCNNNNNNNGC 1 cut(s) 1002
HpyF3I CTNAG 3 cut(s) 276, 687, 967
HpySE526I ACGT 3 cut(s) 328, 384, 1348
Hsp92II CATG 3 cut(s) 266, 798, 980
Ksp22I TGATCA 4 cut(s) 60, 351, 387, 712
Kzo9I GATC 7 cut(s) 60, 222, 351, 387, 712, 856, 1302
LmnI GCTCC 2 cut(s) 579, 964
Lsp1109I GCAGC 3 cut(s) 176, 478, 670
LweI GCATC 1 cut(s) 274
MabI ACCWGGT 1 cut(s) 906
MaeI CTAG 3 cut(s) 248, 393, 761
MaeII ACGT 3 cut(s) 328, 384, 1348
MaeIII GTNAC 2 cut(s) 152, 641
MalI GATC 7 cut(s) 62, 224, 353, 389, 714, 858, 1304
MboI GATC 7 cut(s) 60, 222, 351, 387, 712, 856, 1302
MboII GAAGA 6 cut(s) 421, 580, 626, 649, 899, 1191
MfeI CAATTG 1 cut(s) 554
MhlI GDGCHC 3 cut(s) 55, 372, 1028
MluCI AATT 8 cut(s) 126, 157, 396, 519, 554, 584, 851, 1253
MlyI GAGTC 3 cut(s) 247, 584, 638
MnlI CCTC 7 cut(s) 271, 405, 450, 688, 841, 926, 982
MroXI GAANNNNTTC 1 cut(s) 1107
MseI TTAA 5 cut(s) 294, 380, 672, 815, 900
MspI CCGG 1 cut(s) 765
MspR9I CCNGG 3 cut(s) 56, 908, 1191
MunI CAATTG 1 cut(s) 554
Mva1269I GAATGC 1 cut(s) 167
MvaI CCWGG 3 cut(s) 56, 908, 1191
MwoI GCNNNNNNNGC 1 cut(s) 1002
NdeI CATATG 1 cut(s) 32
NdeII GATC 7 cut(s) 60, 222, 351, 387, 712, 856, 1302
NlaIII CATG 3 cut(s) 266, 798, 980
NlaIV GGNNCC 2 cut(s) 581, 1064
NmeAIII GCCGAG 1 cut(s) 18
NmuCI GTSAC 1 cut(s) 641
NspI RCATGY 1 cut(s) 266
PaqCI CACCTGC 1 cut(s) 1115
PciI ACATGT 1 cut(s) 262
PctI GAATGC 1 cut(s) 167
PdmI GAANNNNTTC 1 cut(s) 1107
PfeI GAWTC 4 cut(s) 751, 773, 1042, 1240
PkrI GCNGC 3 cut(s) 166, 493, 660
PleI GAGTC 3 cut(s) 247, 584, 638
PpsI GAGTC 3 cut(s) 247, 584, 638
PscI ACATGT 1 cut(s) 262
PshBI ATTAAT 1 cut(s) 672
Psp6I CCWGG 3 cut(s) 54, 906, 1189
PspFI CCCAGC 1 cut(s) 404
PspGI CCWGG 3 cut(s) 54, 906, 1189
PspN4I GGNNCC 2 cut(s) 581, 1064
PspPI GGNCC 2 cut(s) 17, 798
PstNI CAGNNNCTG 1 cut(s) 1085
RsaI GTAC 3 cut(s) 146, 267, 416
RsaNI GTAC 3 cut(s) 145, 266, 415
SaqAI TTAA 5 cut(s) 294, 380, 672, 815, 900
SatI GCNGC 3 cut(s) 165, 492, 659
Sau3AI GATC 7 cut(s) 60, 222, 351, 387, 712, 856, 1302
Sau96I GGNCC 2 cut(s) 17, 798
SchI GAGTC 3 cut(s) 247, 584, 638
ScrFI CCNGG 3 cut(s) 56, 908, 1191
SduI GDGCHC 3 cut(s) 55, 372, 1028
SexAI ACCWGGT 1 cut(s) 906
SfaNI GCATC 1 cut(s) 274
SinI GGWCC 2 cut(s) 17, 798
SmlI CTYRAG 1 cut(s) 646
SmoI CTYRAG 1 cut(s) 646
Sse9I AATT 8 cut(s) 126, 157, 396, 519, 554, 584, 851, 1253
SsiI CCGC 1 cut(s) 1005
SspMI CTAG 3 cut(s) 248, 393, 761
StyD4I CCNGG 3 cut(s) 54, 906, 1189
StyI CCWWGG 2 cut(s) 148, 530
TaaI ACNGT 5 cut(s) 701, 802, 865, 928, 1374
TaiI ACGT 3 cut(s) 331, 387, 1351
TaqI TCGA 2 cut(s) 225, 859
TasI AATT 8 cut(s) 126, 157, 396, 519, 554, 584, 851, 1253
TatI WGTACW 1 cut(s) 265
TfiI GAWTC 4 cut(s) 751, 773, 1042, 1240
Tru1I TTAA 5 cut(s) 294, 380, 672, 815, 900
Tru9I TTAA 5 cut(s) 294, 380, 672, 815, 900
TscAI CASTG 5 cut(s) 361, 753, 898, 1284, 1332
TseFI GTSAC 1 cut(s) 641
TseI GCWGC 3 cut(s) 164, 491, 658
Tsp45I GTSAC 1 cut(s) 641
TspDTI ATGAA 7 cut(s) 450, 488, 581, 658, 993, 1046, 1147
TspRI CASTG 5 cut(s) 361, 753, 898, 1284, 1332
VpaK11BI GGWCC 2 cut(s) 17, 798
VspI ATTAAT 1 cut(s) 672
XceI RCATGY 1 cut(s) 266
XmiI GTMKAC 1 cut(s) 1131
XmnI GAANNNNTTC 1 cut(s) 1107
XspI CTAG 3 cut(s) 248, 393, 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.