MD05G1169800.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006974
Physical Location & Seq
Forward (+)
29953760 .. 29954362
603 bp
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UTR
Exon/CDS
Intron
MD05G1169800.v1.1.491

Sequence Viewer

Length: 603 bp
ATGATCGTAGCTGGCTCCGATACCACAGCAACCAGCTTGAAGTGGATCATCTCTTTACTATTAAATAATAAGCATGCTTTGAAGCATGCACAAGAAGAGATAAACCTAAAAGTTGGCAGAGAGAGATGGGTTGAAGACACTGATATCGAAAACCTGACTTATCTCCAAGCCATTGTCAAGGAAACCCTACGTCTATATCCTCCAGGTCCATTCTCAGTCCCACATGAAGCAATGGAAGATTGCCAAGTTTGTGGCCTTCACATTCCAAAGGGCACACGTTTATTTGTCAATTTGTGGAAATTGCACAGAGACCCAACTGTGTGGTCAGAGCCCGAAGTGTTTTCTCCAGATAGGTTTGATGCCTCTGGTCAGCATTTTGAGTTCATTCCATTTGGGTCTGGAAGAAGATCTTGTCCAGGTCTCACATTTGCAATGCAAGTTATCCACTTGAATCTTGGTAGATTGATTCAAGGATTTGAATTGGCTACTCCATTGGACATGTCTGTGGACATGACTGAAGGACTAGGCGTTACTTTGCCAAAGGCAACTTCACTTGAAGTTGTGCTAACTCCACGCTTGCCCATTGAATTCTATGAAAGATAG

Protein Analysis

201

Amino Acids

22.59

Weight (kDa)

5.63

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 1 - 181 1.6e-61 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 53
AcsI RAATTY 1 cut(s) 587
AcuI CTGAAG 1 cut(s) 537
AflIII ACRYGT 2 cut(s) 275, 498
AgsI TTSAA 8 cut(s) 40, 82, 134, 451, 470, 479, 557, 587
AjnI CCWGG 2 cut(s) 202, 415
AjuI GAANNNNNNNTTGG 2 cut(s) 532, 564
AluBI AGCT 2 cut(s) 11, 36
AluI AGCT 2 cut(s) 11, 36
Alw26I GTCTC 2 cut(s) 303, 425
AlwI GGATC 1 cut(s) 53
AoxI GGCC 1 cut(s) 253
ApoI RAATTY 1 cut(s) 587
AspS9I GGNCC 1 cut(s) 206
AvaII GGWCC 1 cut(s) 206
BaeGI GKGCMC 1 cut(s) 275
BanII GRGCYC 1 cut(s) 333
BbsI GAAGAC 1 cut(s) 141
BccI CCATC 1 cut(s) 120
BciT130I CCWGG 2 cut(s) 204, 417
BcoDI GTCTC 2 cut(s) 303, 425
BfaI CTAG 1 cut(s) 524
BglII AGATCT 1 cut(s) 407
Bme1390I CCNGG 2 cut(s) 204, 417
Bme18I GGWCC 1 cut(s) 206
BmgT120I GGNCC 1 cut(s) 206
BmiI GGNNCC 1 cut(s) 16
BmrFI CCNGG 2 cut(s) 204, 417
BmsI GCATC 1 cut(s) 349
BpiI GAAGAC 1 cut(s) 141
BpmI CTGGAG 2 cut(s) 186, 330
BsaI GGTCTC 2 cut(s) 303, 425
Bse3DI GCAATG 2 cut(s) 237, 438
BseBI CCWGG 2 cut(s) 204, 417
BseMI GCAATG 2 cut(s) 237, 438
BseMII CTCAG 1 cut(s) 228
BseSI GKGCMC 1 cut(s) 275
BshFI GGCC 1 cut(s) 255
BslFI GGGAC 1 cut(s) 203
BsmAI GTCTC 2 cut(s) 303, 425
BsmFI GGGAC 1 cut(s) 203
BsnI GGCC 1 cut(s) 255
Bso31I GGTCTC 2 cut(s) 303, 425
Bsp1286I GDGCHC 2 cut(s) 275, 333
Bsp143I GATC 3 cut(s) 3, 45, 407
BspANI GGCC 1 cut(s) 255
BspCNI CTCAG 1 cut(s) 227
BspLI GGNNCC 1 cut(s) 16
BspPI GGATC 1 cut(s) 53
BspTNI GGTCTC 2 cut(s) 303, 425
BsrDI GCAATG 2 cut(s) 237, 438
BssMI GATC 3 cut(s) 3, 45, 407
Bst2UI CCWGG 2 cut(s) 204, 417
Bst4CI ACNGT 1 cut(s) 319
Bst6I CTCTTC 1 cut(s) 90
BstC8I GCNNGC 4 cut(s) 13, 75, 87, 578
BstDEI CTNAG 1 cut(s) 214
BstKTI GATC 3 cut(s) 6, 48, 410
BstMAI GTCTC 2 cut(s) 303, 425
BstMBI GATC 3 cut(s) 3, 45, 407
BstNI CCWGG 2 cut(s) 204, 417
BstNSI RCATGY 3 cut(s) 77, 89, 502
BstSCI CCNGG 2 cut(s) 202, 415
BstSLI GKGCMC 1 cut(s) 275
BstV2I GAAGAC 1 cut(s) 141
BstX2I RGATCY 1 cut(s) 407
BstXI CCANNNNNNTGG 2 cut(s) 251, 321
BstYI RGATCY 1 cut(s) 407
BsuRI GGCC 1 cut(s) 255
BtsIMutI CAGTG 1 cut(s) 138
Cac8I GCNNGC 4 cut(s) 13, 75, 87, 578
Cfr13I GGNCC 1 cut(s) 206
CviAII CATG 5 cut(s) 74, 86, 224, 499, 511
CviJI RGCY 7 cut(s) 11, 15, 36, 170, 255, 331, 485
CviKI_1 RGCY 7 cut(s) 11, 15, 36, 170, 255, 331, 485
DdeI CTNAG 1 cut(s) 214
DpnI GATC 3 cut(s) 5, 47, 409
DpnII GATC 3 cut(s) 3, 45, 407
Eam1104I CTCTTC 1 cut(s) 90
EarI CTCTTC 1 cut(s) 90
Eco24I GRGCYC 1 cut(s) 333
Eco31I GGTCTC 2 cut(s) 303, 425
Eco32I GATATC 1 cut(s) 145
Eco47I GGWCC 1 cut(s) 206
Eco57I CTGAAG 1 cut(s) 537
EcoRI GAATTC 1 cut(s) 587
EcoRII CCWGG 2 cut(s) 202, 415
EcoRV GATATC 1 cut(s) 145
EcoT38I GRGCYC 1 cut(s) 333
FaeI CATG 5 cut(s) 77, 89, 227, 502, 514
FaiI YATR 7 cut(s) 75, 87, 196, 225, 500, 512, 594
FalI AAGNNNNNCTT 2 cut(s) 394, 426
FaqI GGGAC 1 cut(s) 203
FatI CATG 5 cut(s) 73, 85, 223, 498, 510
FriOI GRGCYC 1 cut(s) 333
FspBI CTAG 1 cut(s) 524
GsuI CTGGAG 2 cut(s) 186, 330
HaeIII GGCC 1 cut(s) 255
Hin1II CATG 5 cut(s) 77, 89, 227, 502, 514
HinfI GANTC 2 cut(s) 451, 466
Hpy166II GTNNAC 1 cut(s) 508
Hpy188I TCNGA 2 cut(s) 19, 328
Hpy188III TCNNGA 2 cut(s) 347, 399
Hpy8I GTNNAC 1 cut(s) 508
HpyAV CCTTC 2 cut(s) 266, 512
HpyCH4III ACNGT 1 cut(s) 319
HpyCH4IV ACGT 2 cut(s) 190, 277
HpyCH4V TGCA 4 cut(s) 89, 304, 431, 436
HpyF3I CTNAG 1 cut(s) 214
HpySE526I ACGT 2 cut(s) 190, 277
Hsp92II CATG 5 cut(s) 77, 89, 227, 502, 514
Kzo9I GATC 3 cut(s) 3, 45, 407
LmnI GCTCC 1 cut(s) 20
LpnPI CCDG 9 cut(s) 46, 167, 189, 216, 351, 360, 384, 402, 429
LweI GCATC 1 cut(s) 349
MaeI CTAG 1 cut(s) 524
MaeII ACGT 2 cut(s) 190, 277
MaeIII GTNAC 1 cut(s) 529
MalI GATC 3 cut(s) 5, 47, 409
MboI GATC 3 cut(s) 3, 45, 407
MboII GAAGA 5 cut(s) 107, 146, 248, 414, 417
MflI RGATCY 1 cut(s) 407
MhlI GDGCHC 2 cut(s) 275, 333
MluCI AATT 4 cut(s) 289, 299, 479, 587
MnlI CCTC 2 cut(s) 210, 373
MseI TTAA 1 cut(s) 62
MslI CAYNNNNRTG 1 cut(s) 503
MspR9I CCNGG 2 cut(s) 204, 417
MvaI CCWGG 2 cut(s) 204, 417
NdeII GATC 3 cut(s) 3, 45, 407
NlaIII CATG 5 cut(s) 77, 89, 227, 502, 514
NlaIV GGNNCC 1 cut(s) 16
NspI RCATGY 3 cut(s) 77, 89, 502
PaeI GCATGC 2 cut(s) 77, 89
PciI ACATGT 1 cut(s) 498
PfeI GAWTC 2 cut(s) 451, 466
PscI ACATGT 1 cut(s) 498
Psp6I CCWGG 2 cut(s) 202, 415
PspGI CCWGG 2 cut(s) 202, 415
PspN4I GGNNCC 1 cut(s) 16
PspPI GGNCC 1 cut(s) 206
PsuI RGATCY 1 cut(s) 407
RseI CAYNNNNRTG 1 cut(s) 503
SaqAI TTAA 1 cut(s) 62
Sau3AI GATC 3 cut(s) 3, 45, 407
Sau96I GGNCC 1 cut(s) 206
ScrFI CCNGG 2 cut(s) 204, 417
SduI GDGCHC 2 cut(s) 275, 333
SetI ASST 9 cut(s) 13, 38, 108, 156, 193, 208, 280, 356, 421
SfaNI GCATC 1 cut(s) 349
SinI GGWCC 1 cut(s) 206
SmiMI CAYNNNNRTG 1 cut(s) 503
SphI GCATGC 2 cut(s) 77, 89
Sse9I AATT 4 cut(s) 289, 299, 479, 587
SspMI CTAG 1 cut(s) 524
StyD4I CCNGG 2 cut(s) 202, 415
TaaI ACNGT 1 cut(s) 319
TaiI ACGT 2 cut(s) 193, 280
TaqI TCGA 1 cut(s) 147
TasI AATT 4 cut(s) 289, 299, 479, 587
TfiI GAWTC 2 cut(s) 451, 466
Tru1I TTAA 1 cut(s) 62
Tru9I TTAA 1 cut(s) 62
TscAI CASTG 1 cut(s) 145
TspDTI ATGAA 2 cut(s) 240, 373
TspRI CASTG 1 cut(s) 145
VpaK11BI GGWCC 1 cut(s) 206
XapI RAATTY 1 cut(s) 587
XceI RCATGY 3 cut(s) 77, 89, 502
XcmI CCANNNNNNNNNTGG 1 cut(s) 452
XspI CTAG 1 cut(s) 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.