Rroxscaffold_7G00185140

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0006974
Physical Location & Seq
Reverse (-)
24502566 .. 24507143
4578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00185140.1

Sequence Viewer

Length: 1569 bp
ATGGATTATCTTTCTCATCTACTAGCAATTACAGGACTTCTAATTTTGGTTTCGTTGTATCTATGGAGGGTGAGATTTCAGAATCACAAAATGAAGGGCCTTATGTGCCCAGAACCCTATGGTGCCCTGCCAATTATAGGTCACCTCCATCAACTAGGTGGCCGAAAGCCACTGTTTCGAACCTTAGCAACAATGGCTGATAAGTATGGTCCAATCTTTACCATATGGCTCGGCAAGAACCGTGCCCTTGTGATCAACAACTACGAAGCTGTCAAGGAATGTTTCACCACAAACGACAGGCTTTTCGCCACAAGACCAATTTCTGCACAGGGAGAGTACCTTGGTTACAATTATGCAGCATTCGGGTTCTCACCCTATGGCACATATTGGCGTGATATGAGAAAGTTGGTTGTGATTGAGTTGCTCTCTAATCGTAGGCTAGAGACTCTGAAACATGTACAAGCCTCTGAGGTTGATGCTTTTGTTAAAGGGTTATATTCAGTTTGGGAAAACAAAGGACATGATGGTCAAAGCAAGGTGGTGATCAGTGAATGGATTGAGCACCTCACCTTGAACGTGATCACTAGAATTATTGCTGGGAAGAGGTACTTTGGCAAAGAGAGTGATGAACTTGATGGAGAGCAAAAGAGGATTGGGAAGCTTATGAAGGACTTTATGTATGCTGCTGGCAAACCAGTTGTTTCTGATGTAATTGGGTTTCCAAGGTGGATGGATTTCAAGGGGCAATTGAAAACGATGGAGAATATAGGGAGCCAATTAGACTCTTTGATGACAAGTTGGGTTCAAGAACATCATACAAAGAAGCAGAAGAATGACTCAAGCAACCAGCAGCAGGACTTCATTAATGTCCTGCTCTCTGAGATTGAGGACAGTTCTATGCTTGGTCATACCCGAGATACTATTATCAAGGCAACAGCACTGAATCTCATCCTAGCCGGTTCAGAAACCACATCAATCAGCTTGACGTGGACCGTCTCTTTACTATTAAACAACAGAGATATCTTGAAGCTAGCCCAAGAGGAATTAGATCGAACAGTTGGCAGGCACAAATGGGTGGAAGACACTGACATTAAAAACCTGGTTTACCTCCAAGCCATCGTCAAAGAAACATTGCGTCTATACCCACCAGCTCCCCTCTCAGTTCCACATGAAGCAATGGAGGACTGCAAAGTCGGCGGCTTTGACATCCCGAAGGGCACTCTTTTGTTTGTGAATCTGTGGAAGCTTCATAGGAACCCAAGTGTGTGGCCAGACCCTGAAGCTTTTTCCCCAGAAAGATTTCTTACAAGCCAAGCAGGCGTAGACGCTTCAGGTCAACATTTTGAGTTCATTCCTTTTGGGTCTGGGAGAAGAGTTTGCCCAGGTATCACTTTTGCATTTCAGGTTATACACTTGACGCTGGCAAGGTTGATTCAAGGGTTTGAATTGGCAACTCCATTGGATAAGGCAGTGGATATGACTGAAGGATTGGGGATCACTACACCAAGAGCAACTCCACTAGAAGTTCTTTTGACCCCACGTTTAGCTTCTGAGTTGTATCCACAGTAA

Protein Analysis

522

Amino Acids

59.09

Weight (kDa)

7.72

Isoelectric Point (pI)

33.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 38 - 502 2.2e-100 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 525, 1190
AccB1I GGYRCC 1 cut(s) 122
AccI GTMKAC 1 cut(s) 1323
AciI CCGC 1 cut(s) 1197
AclWI GGATC 1 cut(s) 1502
AcoI YGGCCR 2 cut(s) 160, 1268
AcuI CTGAAG 3 cut(s) 1299, 1314, 1503
AfaI GTAC 3 cut(s) 338, 459, 608
AfiI CCNNNNNNNGG 2 cut(s) 137, 853
AflIII ACRYGT 1 cut(s) 454
AgsI TTSAA 7 cut(s) 574, 739, 751, 806, 1027, 1436, 1445
AjiI CACGTC 1 cut(s) 987
AjnI CCWGG 2 cut(s) 1098, 1381
AluBI AGCT 8 cut(s) 269, 661, 981, 1030, 1151, 1246, 1283, 1547
AluI AGCT 8 cut(s) 269, 661, 981, 1030, 1151, 1246, 1283, 1547
Alw21I GWGCWC 1 cut(s) 564
Alw26I GTCTC 2 cut(s) 437, 1000
AlwI GGATC 1 cut(s) 1502
AlwNI CAGNNNCTG 1 cut(s) 1277
Ama87I CYCGRG 1 cut(s) 912
AoxI GGCC 3 cut(s) 97, 160, 1268
ApeKI GCWGC 3 cut(s) 356, 683, 850
ArsI GACNNNNNNTTYG 2 cut(s) 287, 319
AseI ATTAAT 1 cut(s) 864
Asp700I GAANNNNTTC 1 cut(s) 1299
AspS9I GGNCC 3 cut(s) 97, 209, 990
AsuHPI GGTGA 6 cut(s) 82, 134, 277, 363, 553, 559
AsuII TTCGAA 1 cut(s) 178
AsuNHI GCTAGC 1 cut(s) 1030
AvaI CYCGRG 1 cut(s) 912
AvaII GGWCC 2 cut(s) 209, 990
BaeGI GKGCMC 4 cut(s) 110, 127, 247, 1220
BalI TGGCCA 1 cut(s) 1270
BanI GGYRCC 1 cut(s) 122
BbsI GAAGAC 1 cut(s) 1086
Bbv12I GWGCWC 1 cut(s) 564
BbvI GCAGC 3 cut(s) 368, 670, 862
BccI CCATC 6 cut(s) 156, 518, 629, 724, 751, 1124
BciT130I CCWGG 2 cut(s) 1100, 1383
BciVI GTATCC 1 cut(s) 1569
BclI TGATCA 3 cut(s) 252, 543, 579
BcoDI GTCTC 2 cut(s) 437, 1000
BfaI CTAG 7 cut(s) 23, 155, 440, 585, 953, 1031, 1520
BfuI GTATCC 1 cut(s) 1569
BglI GCCNNNNNGGC 1 cut(s) 1317
BisI GCNGC 4 cut(s) 357, 684, 851, 1198
BlsI GCNGC 4 cut(s) 358, 685, 852, 1199
Bme1390I CCNGG 2 cut(s) 1100, 1383
Bme18I GGWCC 2 cut(s) 209, 990
BmeT110I CYCGRG 1 cut(s) 912
BmgBI CACGTC 1 cut(s) 987
BmgT120I GGNCC 3 cut(s) 97, 209, 990
BmiI GGNNCC 3 cut(s) 124, 773, 1256
BmrFI CCNGG 2 cut(s) 1100, 1383
BmsI GCATC 1 cut(s) 466
BmtI GCTAGC 1 cut(s) 1034
BpiI GAAGAC 1 cut(s) 1086
BplI GAGNNNNNCTC 2 cut(s) 410, 442
Bpu10I CCTNAGC 1 cut(s) 184
Bpu14I TTCGAA 1 cut(s) 178
BpuEI CTTGAG 1 cut(s) 823
BsaJI CCNNGG 3 cut(s) 340, 722, 1381
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 853
Bse118I RCCGGY 1 cut(s) 956
Bse1I ACTGG 1 cut(s) 695
Bse3DI GCAATG 2 cut(s) 1130, 1182
BseBI CCWGG 2 cut(s) 1100, 1383
BseDI CCNNGG 3 cut(s) 340, 722, 1381
BseGI GGATG 3 cut(s) 735, 948, 1206
BseLI CCNNNNNNNGG 2 cut(s) 137, 853
BseMI GCAATG 2 cut(s) 1130, 1182
BseMII CTCAG 4 cut(s) 459, 870, 1173, 1542
BseNI ACTGG 1 cut(s) 695
BseSI GKGCMC 4 cut(s) 110, 127, 247, 1220
BseXI GCAGC 3 cut(s) 368, 670, 862
BseYI CCCAGC 1 cut(s) 596
BsgI GTGCAG 1 cut(s) 309
BshFI GGCC 3 cut(s) 99, 162, 1270
BshNI GGYRCC 1 cut(s) 122
BsiHKAI GWGCWC 1 cut(s) 564
BsiHKCI CYCGRG 1 cut(s) 912
BsiSI CCGG 1 cut(s) 957
BslI CCNNNNNNNGG 2 cut(s) 137, 853
BsmAI GTCTC 2 cut(s) 437, 1000
BsmBI CGTCTC 1 cut(s) 1000
BsmI GAATGC 1 cut(s) 359
BsnI GGCC 3 cut(s) 99, 162, 1270
BsoBI CYCGRG 1 cut(s) 912
Bsp119I TTCGAA 1 cut(s) 178
Bsp1286I GDGCHC 5 cut(s) 110, 127, 247, 564, 1220
Bsp1407I TGTACA 1 cut(s) 457
Bsp143I GATC 5 cut(s) 252, 543, 579, 1048, 1494
BspACI CCGC 1 cut(s) 1197
BspANI GGCC 3 cut(s) 99, 162, 1270
BspCNI CTCAG 4 cut(s) 460, 871, 1172, 1543
BspLI GGNNCC 3 cut(s) 124, 773, 1256
BspOI GCTAGC 1 cut(s) 1034
BspPI GGATC 1 cut(s) 1502
BspT104I TTCGAA 1 cut(s) 178
BspT107I GGYRCC 1 cut(s) 122
BsrDI GCAATG 2 cut(s) 1130, 1182
BsrFI RCCGGY 1 cut(s) 956
BsrGI TGTACA 1 cut(s) 457
BsrI ACTGG 1 cut(s) 695
BssAI RCCGGY 1 cut(s) 956
BssECI CCNNGG 3 cut(s) 340, 722, 1381
BssMI GATC 5 cut(s) 252, 543, 579, 1048, 1494
BssT1I CCWWGG 2 cut(s) 340, 722
Bst2UI CCWGG 2 cut(s) 1100, 1383
Bst4CI ACNGT 6 cut(s) 174, 242, 893, 994, 1057, 1566
Bst6I CTCTTC 2 cut(s) 596, 1366
BstAUI TGTACA 1 cut(s) 457
BstBI TTCGAA 1 cut(s) 178
BstC8I GCNNGC 5 cut(s) 688, 1032, 1064, 1318, 1422
BstDEI CTNAG 5 cut(s) 184, 468, 879, 1159, 1551
BstEII GGTNACC 1 cut(s) 140
BstF5I GGATG 3 cut(s) 735, 948, 1206
BstKTI GATC 5 cut(s) 255, 546, 582, 1051, 1497
BstMAI GTCTC 2 cut(s) 437, 1000
BstMBI GATC 5 cut(s) 252, 543, 579, 1048, 1494
BstMWI GCNNNNNNNGC 4 cut(s) 105, 194, 1194, 1317
BstNI CCWGG 2 cut(s) 1100, 1383
BstNSI RCATGY 1 cut(s) 458
BstPI GGTNACC 1 cut(s) 140
BstSCI CCNGG 2 cut(s) 1098, 1381
BstSLI GKGCMC 4 cut(s) 110, 127, 247, 1220
BstV1I GCAGC 3 cut(s) 368, 670, 862
BstV2I GAAGAC 1 cut(s) 1086
BstXI CCANNNNNNTGG 1 cut(s) 1266
BsuI GTATCC 1 cut(s) 1569
BsuRI GGCC 3 cut(s) 99, 162, 1270
BtrI CACGTC 1 cut(s) 987
BtsCI GGATG 3 cut(s) 735, 948, 1206
BtsI GCAGTG 1 cut(s) 1476
BtsIMutI CAGTG 5 cut(s) 170, 553, 938, 1083, 1476
Cac8I GCNNGC 5 cut(s) 688, 1032, 1064, 1318, 1422
CaiI CAGNNNCTG 1 cut(s) 1277
Cfr10I RCCGGY 1 cut(s) 956
Cfr13I GGNCC 3 cut(s) 97, 209, 990
CseI GACGC 3 cut(s) 1124, 1334, 1426
CsiI ACCWGGT 1 cut(s) 1098
Csp6I GTAC 3 cut(s) 337, 458, 607
CviAII CATG 3 cut(s) 455, 521, 1169
CviQI GTAC 3 cut(s) 337, 458, 607
DdeI CTNAG 5 cut(s) 184, 468, 879, 1159, 1551
DpnI GATC 5 cut(s) 254, 545, 581, 1050, 1496
DpnII GATC 5 cut(s) 252, 543, 579, 1048, 1494
DrdI GACNNNNNNGTC 2 cut(s) 525, 1190
DseDI GACNNNNNNGTC 2 cut(s) 525, 1190
EaeI YGGCCR 2 cut(s) 160, 1268
Eam1104I CTCTTC 2 cut(s) 596, 1366
EarI CTCTTC 2 cut(s) 596, 1366
Eco130I CCWWGG 2 cut(s) 340, 722
Eco32I GATATC 1 cut(s) 1021
Eco47I GGWCC 2 cut(s) 209, 990
Eco57I CTGAAG 3 cut(s) 1299, 1314, 1503
Eco88I CYCGRG 1 cut(s) 912
Eco91I GGTNACC 1 cut(s) 140
EcoO109I RGGNCCY 1 cut(s) 97
EcoO65I GGTNACC 1 cut(s) 140
EcoRII CCWGG 2 cut(s) 1098, 1381
EcoRV GATATC 1 cut(s) 1021
EcoT14I CCWWGG 2 cut(s) 340, 722
ErhI CCWWGG 2 cut(s) 340, 722
Esp3I CGTCTC 1 cut(s) 1000
FaeI CATG 3 cut(s) 458, 524, 1172
FalI AAGNNNNNCTT 2 cut(s) 593, 625
FatI CATG 3 cut(s) 454, 520, 1168
FauNDI CATATG 1 cut(s) 224
FbaI TGATCA 3 cut(s) 252, 543, 579
FblI GTMKAC 1 cut(s) 1323
Fnu4HI GCNGC 4 cut(s) 357, 684, 851, 1198
FokI GGATG 3 cut(s) 742, 935, 1193
Fsp4HI GCNGC 4 cut(s) 357, 684, 851, 1198
FspBI CTAG 7 cut(s) 23, 155, 440, 585, 953, 1031, 1520
GluI GCNGC 4 cut(s) 357, 684, 851, 1198
GsaI CCCAGC 1 cut(s) 600
HaeIII GGCC 3 cut(s) 99, 162, 1270
HapII CCGG 1 cut(s) 957
HgaI GACGC 3 cut(s) 1124, 1334, 1426
Hin1II CATG 3 cut(s) 458, 524, 1172
HincII GTYRAC 1 cut(s) 1337
HindII GTYRAC 1 cut(s) 1337
HindIII AAGCTT 3 cut(s) 659, 1244, 1281
HinfI GANTC 7 cut(s) 82, 445, 782, 836, 943, 1234, 1432
HpaII CCGG 1 cut(s) 957
HphI GGTGA 6 cut(s) 82, 134, 277, 363, 553, 559
Hpy166II GTNNAC 4 cut(s) 990, 1105, 1324, 1337
Hpy188I TCNGA 7 cut(s) 81, 450, 469, 706, 880, 964, 1552
Hpy188III TCNNGA 3 cut(s) 806, 1024, 1210
Hpy8I GTNNAC 4 cut(s) 990, 1105, 1324, 1337
HpyAV CCTTC 4 cut(s) 88, 661, 1207, 1478
HpyCH4III ACNGT 6 cut(s) 174, 242, 893, 994, 1057, 1566
HpyCH4IV ACGT 3 cut(s) 576, 986, 1540
HpyCH4V TGCA 4 cut(s) 326, 356, 1188, 1397
HpyF10VI GCNNNNNNNGC 4 cut(s) 105, 194, 1194, 1317
HpyF3I CTNAG 5 cut(s) 184, 468, 879, 1159, 1551
HpySE526I ACGT 3 cut(s) 576, 986, 1540
Hsp92II CATG 3 cut(s) 458, 524, 1172
Ksp22I TGATCA 3 cut(s) 252, 543, 579
Kzo9I GATC 5 cut(s) 252, 543, 579, 1048, 1494
LmnI GCTCC 2 cut(s) 771, 1156
Lsp1109I GCAGC 3 cut(s) 368, 670, 862
LweI GCATC 1 cut(s) 466
MabI ACCWGGT 1 cut(s) 1098
MaeI CTAG 7 cut(s) 23, 155, 440, 585, 953, 1031, 1520
MaeII ACGT 3 cut(s) 576, 986, 1540
MaeIII GTNAC 2 cut(s) 140, 344
MalI GATC 5 cut(s) 254, 545, 581, 1050, 1496
MboI GATC 5 cut(s) 252, 543, 579, 1048, 1494
MboII GAAGA 4 cut(s) 613, 841, 1091, 1383
MfeI CAATTG 1 cut(s) 746
MhlI GDGCHC 5 cut(s) 110, 127, 247, 564, 1220
MlsI TGGCCA 1 cut(s) 1270
MluNI TGGCCA 1 cut(s) 1270
MlyI GAGTC 3 cut(s) 439, 776, 830
Mox20I TGGCCA 1 cut(s) 1270
MroXI GAANNNNTTC 1 cut(s) 1299
MscI TGGCCA 1 cut(s) 1270
MseI TTAA 4 cut(s) 486, 864, 1007, 1092
Msp20I TGGCCA 1 cut(s) 1270
MspI CCGG 1 cut(s) 957
MspR9I CCNGG 2 cut(s) 1100, 1383
MunI CAATTG 1 cut(s) 746
Mva1269I GAATGC 1 cut(s) 359
MvaI CCWGG 2 cut(s) 1100, 1383
MwoI GCNNNNNNNGC 4 cut(s) 105, 194, 1194, 1317
NdeI CATATG 1 cut(s) 224
NdeII GATC 5 cut(s) 252, 543, 579, 1048, 1494
NheI GCTAGC 1 cut(s) 1030
NlaIII CATG 3 cut(s) 458, 524, 1172
NlaIV GGNNCC 3 cut(s) 124, 773, 1256
NmeAIII GCCGAG 1 cut(s) 210
NmuCI GTSAC 1 cut(s) 140
NspI RCATGY 1 cut(s) 458
NspV TTCGAA 1 cut(s) 178
PciI ACATGT 1 cut(s) 454
PctI GAATGC 1 cut(s) 359
PdmI GAANNNNTTC 1 cut(s) 1299
PfeI GAWTC 4 cut(s) 82, 943, 1234, 1432
PkrI GCNGC 4 cut(s) 358, 685, 852, 1199
PleI GAGTC 3 cut(s) 439, 776, 830
PpsI GAGTC 3 cut(s) 439, 776, 830
PscI ACATGT 1 cut(s) 454
PshBI ATTAAT 1 cut(s) 864
Psp6I CCWGG 2 cut(s) 1098, 1381
PspEI GGTNACC 1 cut(s) 140
PspFI CCCAGC 1 cut(s) 596
PspGI CCWGG 2 cut(s) 1098, 1381
PspN4I GGNNCC 3 cut(s) 124, 773, 1256
PspPI GGNCC 3 cut(s) 97, 209, 990
PstNI CAGNNNCTG 1 cut(s) 1277
RsaI GTAC 3 cut(s) 338, 459, 608
RsaNI GTAC 3 cut(s) 337, 458, 607
SaqAI TTAA 4 cut(s) 486, 864, 1007, 1092
SatI GCNGC 4 cut(s) 357, 684, 851, 1198
Sau3AI GATC 5 cut(s) 252, 543, 579, 1048, 1494
Sau96I GGNCC 3 cut(s) 97, 209, 990
SchI GAGTC 3 cut(s) 439, 776, 830
ScrFI CCNGG 2 cut(s) 1100, 1383
SduI GDGCHC 5 cut(s) 110, 127, 247, 564, 1220
SexAI ACCWGGT 1 cut(s) 1098
SfaNI GCATC 1 cut(s) 466
SfuI TTCGAA 1 cut(s) 178
SinI GGWCC 2 cut(s) 209, 990
SmlI CTYRAG 1 cut(s) 838
SmoI CTYRAG 1 cut(s) 838
SsiI CCGC 1 cut(s) 1197
SspMI CTAG 7 cut(s) 23, 155, 440, 585, 953, 1031, 1520
StyD4I CCNGG 2 cut(s) 1098, 1381
StyI CCWWGG 2 cut(s) 340, 722
TaaI ACNGT 6 cut(s) 174, 242, 893, 994, 1057, 1566
TaiI ACGT 3 cut(s) 579, 989, 1543
TaqI TCGA 2 cut(s) 178, 1051
TatI WGTACW 1 cut(s) 457
TauI GCSGC 1 cut(s) 1200
TfiI GAWTC 4 cut(s) 82, 943, 1234, 1432
Tru1I TTAA 4 cut(s) 486, 864, 1007, 1092
Tru9I TTAA 4 cut(s) 486, 864, 1007, 1092
TscAI CASTG 5 cut(s) 177, 553, 945, 1090, 1476
TseFI GTSAC 1 cut(s) 140
TseI GCWGC 3 cut(s) 356, 683, 850
Tsp45I GTSAC 1 cut(s) 140
TspDTI ATGAA 7 cut(s) 107, 642, 680, 850, 1185, 1238, 1339
TspRI CASTG 5 cut(s) 177, 553, 945, 1090, 1476
VpaK11BI GGWCC 2 cut(s) 209, 990
VspI ATTAAT 1 cut(s) 864
XceI RCATGY 1 cut(s) 458
XcmI CCANNNNNNNNNTGG 1 cut(s) 155
XmiI GTMKAC 1 cut(s) 1323
XmnI GAANNNNTTC 1 cut(s) 1299
XspI CTAG 7 cut(s) 23, 155, 440, 585, 953, 1031, 1520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.