FvH4_2g25530

WD repeat-containing protein 89 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009953
Physical Location & Seq
Reverse (-)
20676290 .. 20679324
3035 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g25530.t1

Sequence Viewer

Length: 1167 bp
ATGGAAGCCATGGACTTGGATATGGAGCCAAAACCTCAACCTGAGTCTTCATCCAATCCAAACTCCTTCAAACGTTTCGGCCTCAAAACCTCCATCCAAACCAACTTTGGAGACGATTACGTCTTCCAAATTGTCCCCAAGGATGACTGGACAGCAATGGCGGTGTCATTGTCAACGAACGCAGTGAAGCTCTACTCACCGGTGACGGGTCAGTACTATGGAGAGTGCAAAGGCCACTCTGCAACAATCAATCACATCTCATTTGCAGACTCATCATCCCCACATGTTCTGCACTCTTGCTCTTCCGACGGAACCATTAGGCTTTGGGACACCCGCACATTCCAACAGGTGTCGTCTTTTCCTTCTGGTTCGGGTGCTGAGATATTCAGCTTTTCGTTTGGAGGATCATCTAATAATCTTCTAGCTGCAGGATGTAATTCTCAGATACTATTGTGGGATTGGAGGAACGACAAACAAGTAGCCTGCTTAGAGGACTCTCATGTGGAAGATGTTACTCAGGTTCACTTCATTCCTGACCATCAAAGCAAGCTCCTTTCTGCTTCTGTAGATGGATTGATATGTATATTTGATACAGATGGAGATATCAACGAAGATGATCATCTTGAGTCGGTCCTAAATGTGGGCACCTCCATCGGGAAGGTGGGGTTTTTTGGTGAGACATATCAAAAGCTTTGGTGTCTGACCCATATTGAAACCTTAAGTATCTGGGACTGGAAGGATGCAACTCAAACAAATTTCCAGGACGCTCGTTCTTTGGCTTCTAAAAGTTGGAGTCTAGGTGATGTTGATTACTTTGTTGATTGCCACTACTCAAGAGAAGCAGAACGCTTATGGCTTATTGGTGGTGCTAACACTGGTGCTTTAGGCTACTTCCCTGTAAGTTATTCAGGAACGCAGGCCATAGGTACCGCTGAAGCAATTTTTGAAGGTGGCCATACGGGTGTTGTTCGAAGTGTGTTGCCTATGTCAAACATGCCAGGTCCCCAAGGCCAGAGTTTTTTTGGATGGACTGGTGGTGAAGATGGCCGTTTATGTTGCTGGCTATCAGATGGTTCTGTGGACACCATAAACCGATCATGGATTTCAAGCGAACTCGTTTTGAGATCACCAAAGATCCGCAAGTCAAAAAGGCATAGCCCTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010029 GO:0040008 GO:0048580 GO:0050789 GO:0050793 GO:0051239 GO:0065007 GO:1900140 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

389

Amino Acids

42.76

Weight (kDa)

4.97

Isoelectric Point (pI)

45.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 34 - 250 7.1e-12 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 40 - 199 5.4e-10 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 41 - 112 1.8e-06 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 51 - 199 5.9e-23 WDR5 beta-propeller domain
WDR55 PF24796 51 - 248 8.5e-10 WDR55
Beta-prop_WDR3_1st PF25173 55 - 199 1.9e-16 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 60 - 124 2.1e-08 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 60 - 154 2.7e-07 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 61 - 197 3e-12 Prp19 WD40 domain
WD40_Gbeta PF25391 75 - 201 5.9e-08 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 96 - 208 5e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_EIPR1 PF23609 118 - 198 6.9e-06 EIPR1 beta-propeller
Beta-prop_THOC3 PF25174 132 - 199 6.8e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
Acc65I GGTACC 1 cut(s) 926
AccB1I GGYRCC 2 cut(s) 644, 926
AciI CCGC 4 cut(s) 161, 334, 930, 1138
AclI AACGTT 1 cut(s) 73
AclWI GGATC 2 cut(s) 412, 1129
AcoI YGGCCR 2 cut(s) 952, 1045
AcsI RAATTY 1 cut(s) 754
AcuI CTGAAG 1 cut(s) 954
AfaI GTAC 2 cut(s) 215, 928
AfiI CCNNNNNNNGG 3 cut(s) 206, 640, 654
AflII CTTAAG 1 cut(s) 718
AflIII ACRYGT 1 cut(s) 283
AgeI ACCGGT 1 cut(s) 199
AgsI TTSAA 4 cut(s) 70, 713, 947, 1107
AjnI CCWGG 2 cut(s) 759, 997
AluBI AGCT 5 cut(s) 190, 390, 425, 550, 691
AluI AGCT 5 cut(s) 190, 390, 425, 550, 691
Alw26I GTCTC 2 cut(s) 105, 671
AlwI GGATC 2 cut(s) 412, 1129
AoxI GGCC 6 cut(s) 79, 232, 918, 952, 1009, 1045
ApeKI GCWGC 1 cut(s) 425
ApoI RAATTY 1 cut(s) 754
AsiGI ACCGGT 1 cut(s) 199
Asp718I GGTACC 1 cut(s) 926
AspS9I GGNCC 2 cut(s) 631, 1001
AsuHPI GGTGA 6 cut(s) 189, 214, 686, 812, 1049, 1119
AsuII TTCGAA 1 cut(s) 970
AvaII GGWCC 2 cut(s) 631, 1001
BaeGI GKGCMC 1 cut(s) 647
BaeI ACNNNNGTAYC 2 cut(s) 437, 470
BalI TGGCCA 1 cut(s) 954
BanI GGYRCC 2 cut(s) 644, 926
BbsI GAAGAC 2 cut(s) 39, 115
BbvI GCAGC 1 cut(s) 412
BccI CCATC 8 cut(s) 101, 546, 563, 590, 659, 1020, 1037, 1064
BceAI ACGGC 1 cut(s) 1032
BcgI CGANNNNNNTGC 2 cut(s) 634, 668
BciT130I CCWGG 2 cut(s) 761, 999
BclI TGATCA 1 cut(s) 616
BcoDI GTCTC 2 cut(s) 105, 671
BfaI CTAG 3 cut(s) 422, 797, 1165
BfmI CTRYAG 2 cut(s) 426, 564
BfrI CTTAAG 1 cut(s) 718
BisI GCNGC 1 cut(s) 426
BlsI GCNGC 1 cut(s) 427
BmcAI AGTACT 1 cut(s) 215
Bme1390I CCNGG 2 cut(s) 761, 999
Bme18I GGWCC 2 cut(s) 631, 1001
BmgT120I GGNCC 2 cut(s) 631, 1001
BmiI GGNNCC 5 cut(s) 27, 313, 646, 928, 1003
BmrFI CCNGG 2 cut(s) 761, 999
BmsI GCATC 1 cut(s) 730
BpiI GAAGAC 2 cut(s) 39, 115
Bpu14I TTCGAA 1 cut(s) 970
BpuEI CTTGAG 2 cut(s) 644, 817
BsaBI GATNNNNATC 1 cut(s) 618
BsaJI CCNNGG 3 cut(s) 9, 138, 1006
BsaWI WCCGGW 1 cut(s) 199
BsaXI ACNNNNNCTCC 2 cut(s) 784, 814
Bsc4I CCNNNNNNNGG 3 cut(s) 206, 640, 654
Bse118I RCCGGY 1 cut(s) 199
Bse1I ACTGG 4 cut(s) 152, 737, 880, 1036
Bse3DI GCAATG 1 cut(s) 162
Bse8I GATNNNNATC 1 cut(s) 618
BseBI CCWGG 2 cut(s) 761, 999
BseDI CCNNGG 3 cut(s) 9, 138, 1006
BseGI GGATG 7 cut(s) 50, 93, 148, 275, 437, 745, 1031
BseJI GATNNNNATC 1 cut(s) 618
BseLI CCNNNNNNNGG 3 cut(s) 206, 640, 654
BseMI GCAATG 1 cut(s) 162
BseMII CTCAG 4 cut(s) 33, 369, 455, 530
BseNI ACTGG 4 cut(s) 152, 737, 880, 1036
BseSI GKGCMC 1 cut(s) 647
BseXI GCAGC 1 cut(s) 412
BsgI GTGCAG 1 cut(s) 275
BshFI GGCC 6 cut(s) 81, 234, 920, 954, 1011, 1047
BshNI GGYRCC 2 cut(s) 644, 926
BshTI ACCGGT 1 cut(s) 199
BsiSI CCGG 1 cut(s) 200
BslFI GGGAC 4 cut(s) 119, 341, 743, 987
BslI CCNNNNNNNGG 3 cut(s) 206, 640, 654
BsmAI GTCTC 2 cut(s) 105, 671
BsmBI CGTCTC 1 cut(s) 105
BsmFI GGGAC 4 cut(s) 119, 341, 743, 987
BsnI GGCC 6 cut(s) 81, 234, 920, 954, 1011, 1047
Bsp119I TTCGAA 1 cut(s) 970
Bsp1286I GDGCHC 1 cut(s) 647
Bsp143I GATC 5 cut(s) 404, 616, 1094, 1124, 1134
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 4 cut(s) 161, 334, 930, 1138
BspANI GGCC 6 cut(s) 81, 234, 920, 954, 1011, 1047
BspCNI CTCAG 4 cut(s) 34, 370, 454, 529
BspLI GGNNCC 5 cut(s) 27, 313, 646, 928, 1003
BspMAI CTGCAG 1 cut(s) 430
BspPI GGATC 2 cut(s) 412, 1129
BspQI GCTCTTC 1 cut(s) 307
BspT104I TTCGAA 1 cut(s) 970
BspT107I GGYRCC 2 cut(s) 644, 926
BspTI CTTAAG 1 cut(s) 718
BsrDI GCAATG 1 cut(s) 162
BsrFI RCCGGY 1 cut(s) 199
BsrI ACTGG 4 cut(s) 152, 737, 880, 1036
BssAI RCCGGY 1 cut(s) 199
BssECI CCNNGG 3 cut(s) 9, 138, 1006
BssMI GATC 5 cut(s) 404, 616, 1094, 1124, 1134
BssT1I CCWWGG 3 cut(s) 9, 138, 1006
Bst2UI CCWGG 2 cut(s) 761, 999
Bst6I CTCTTC 1 cut(s) 307
BstAFI CTTAAG 1 cut(s) 718
BstBI TTCGAA 1 cut(s) 970
BstC8I GCNNGC 4 cut(s) 484, 548, 918, 1061
BstDEI CTNAG 5 cut(s) 42, 378, 441, 487, 516
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 7 cut(s) 50, 93, 148, 275, 437, 745, 1031
BstKTI GATC 5 cut(s) 407, 619, 1097, 1127, 1137
BstMAI GTCTC 2 cut(s) 105, 671
BstMBI GATC 5 cut(s) 404, 616, 1094, 1124, 1134
BstNI CCWGG 2 cut(s) 761, 999
BstNSI RCATGY 2 cut(s) 287, 997
BstSCI CCNGG 2 cut(s) 759, 997
BstSFI CTRYAG 2 cut(s) 426, 564
BstSLI GKGCMC 1 cut(s) 647
BstV1I GCAGC 1 cut(s) 412
BstV2I GAAGAC 2 cut(s) 39, 115
BstX2I RGATCY 1 cut(s) 1134
BstXI CCANNNNNNTGG 1 cut(s) 16
BstYI RGATCY 1 cut(s) 1134
BsuRI GGCC 6 cut(s) 81, 234, 920, 954, 1011, 1047
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 7 cut(s) 50, 93, 148, 275, 437, 745, 1031
BtsI GCAGTG 1 cut(s) 189
BtsIMutI CAGTG 2 cut(s) 189, 873
Cac8I GCNNGC 4 cut(s) 484, 548, 918, 1061
Cfr10I RCCGGY 1 cut(s) 199
Cfr13I GGNCC 2 cut(s) 631, 1001
CseI GACGC 1 cut(s) 773
Csp6I GTAC 2 cut(s) 214, 927
CspAI ACCGGT 1 cut(s) 199
CviAII CATG 5 cut(s) 10, 284, 500, 994, 1098
CviQI GTAC 2 cut(s) 214, 927
DdeI CTNAG 5 cut(s) 42, 378, 441, 487, 516
DpnI GATC 5 cut(s) 406, 618, 1096, 1126, 1136
DpnII GATC 5 cut(s) 404, 616, 1094, 1124, 1134
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
EaeI YGGCCR 2 cut(s) 952, 1045
Eam1104I CTCTTC 1 cut(s) 307
EarI CTCTTC 1 cut(s) 307
Eco130I CCWWGG 3 cut(s) 9, 138, 1006
Eco32I GATATC 1 cut(s) 604
Eco47I GGWCC 2 cut(s) 631, 1001
Eco57I CTGAAG 1 cut(s) 954
EcoO109I RGGNCCY 1 cut(s) 1001
EcoRII CCWGG 2 cut(s) 759, 997
EcoRV GATATC 1 cut(s) 604
EcoT14I CCWWGG 3 cut(s) 9, 138, 1006
ErhI CCWWGG 3 cut(s) 9, 138, 1006
Esp3I CGTCTC 1 cut(s) 105
FaeI CATG 5 cut(s) 13, 287, 503, 997, 1101
FaqI GGGAC 4 cut(s) 119, 341, 743, 987
FatI CATG 5 cut(s) 9, 283, 499, 993, 1097
FauI CCCGC 1 cut(s) 341
FbaI TGATCA 1 cut(s) 616
Fnu4HI GCNGC 1 cut(s) 426
FokI GGATG 7 cut(s) 37, 80, 155, 262, 444, 752, 1038
Fsp4HI GCNGC 1 cut(s) 426
FspBI CTAG 3 cut(s) 422, 797, 1165
GluI GCNGC 1 cut(s) 426
HaeIII GGCC 6 cut(s) 81, 234, 920, 954, 1011, 1047
HapII CCGG 1 cut(s) 200
HgaI GACGC 1 cut(s) 773
Hin1II CATG 5 cut(s) 13, 287, 503, 997, 1101
HincII GTYRAC 1 cut(s) 174
HindII GTYRAC 1 cut(s) 174
HindIII AAGCTT 1 cut(s) 689
HinfI GANTC 5 cut(s) 44, 269, 494, 626, 793
HpaII CCGG 1 cut(s) 200
HphI GGTGA 6 cut(s) 189, 214, 686, 812, 1049, 1119
Hpy166II GTNNAC 3 cut(s) 174, 523, 1081
Hpy188I TCNGA 4 cut(s) 307, 444, 702, 1069
Hpy188III TCNNGA 5 cut(s) 533, 623, 655, 834, 909
Hpy8I GTNNAC 3 cut(s) 174, 523, 1081
Hpy99I CGWCG 1 cut(s) 311
HpyAV CCTTC 5 cut(s) 76, 372, 652, 730, 941
HpyCH4IV ACGT 2 cut(s) 73, 120
HpyCH4V TGCA 6 cut(s) 228, 242, 266, 292, 428, 743
HpyF3I CTNAG 5 cut(s) 42, 378, 441, 487, 516
HpySE526I ACGT 2 cut(s) 73, 120
Hsp92II CATG 5 cut(s) 13, 287, 503, 997, 1101
KpnI GGTACC 1 cut(s) 930
Ksp22I TGATCA 1 cut(s) 616
Kzo9I GATC 5 cut(s) 404, 616, 1094, 1124, 1134
LguI GCTCTTC 1 cut(s) 307
LmnI GCTCC 2 cut(s) 25, 555
Lsp1109I GCAGC 1 cut(s) 412
LweI GCATC 1 cut(s) 730
MaeI CTAG 3 cut(s) 422, 797, 1165
MaeII ACGT 2 cut(s) 73, 120
MaeIII GTNAC 2 cut(s) 202, 511
MalI GATC 5 cut(s) 406, 618, 1096, 1126, 1136
MboI GATC 5 cut(s) 404, 616, 1094, 1124, 1134
MboII GAAGA 7 cut(s) 39, 115, 294, 410, 518, 623, 1052
MflI RGATCY 1 cut(s) 1134
MhlI GDGCHC 1 cut(s) 647
MlsI TGGCCA 1 cut(s) 954
MluCI AATT 4 cut(s) 129, 436, 754, 939
MluNI TGGCCA 1 cut(s) 954
MlyI GAGTC 5 cut(s) 53, 263, 488, 635, 802
MmeI TCCRAC 3 cut(s) 330, 367, 770
MnlI CCTC 7 cut(s) 45, 92, 100, 395, 456, 484, 658
Mox20I TGGCCA 1 cut(s) 954
MscI TGGCCA 1 cut(s) 954
MseI TTAA 1 cut(s) 719
MslI CAYNNNNRTG 1 cut(s) 960
Msp20I TGGCCA 1 cut(s) 954
MspA1I CMGCKG 1 cut(s) 932
MspCI CTTAAG 1 cut(s) 718
MspI CCGG 1 cut(s) 200
MspR9I CCNGG 2 cut(s) 761, 999
MvaI CCWGG 2 cut(s) 761, 999
NcoI CCATGG 1 cut(s) 9
NdeII GATC 5 cut(s) 404, 616, 1094, 1124, 1134
NlaIII CATG 5 cut(s) 13, 287, 503, 997, 1101
NlaIV GGNNCC 5 cut(s) 27, 313, 646, 928, 1003
NmuCI GTSAC 1 cut(s) 202
NspI RCATGY 2 cut(s) 287, 997
NspV TTCGAA 1 cut(s) 970
PciI ACATGT 1 cut(s) 283
PciSI GCTCTTC 1 cut(s) 307
PfoI TCCNGGA 1 cut(s) 759
PinAI ACCGGT 1 cut(s) 199
PkrI GCNGC 1 cut(s) 427
PleI GAGTC 5 cut(s) 52, 263, 488, 634, 801
PpsI GAGTC 5 cut(s) 52, 263, 488, 634, 801
PpuMI RGGWCCY 1 cut(s) 1001
PscI ACATGT 1 cut(s) 283
Psp1406I AACGTT 1 cut(s) 73
Psp5II RGGWCCY 1 cut(s) 1001
Psp6I CCWGG 2 cut(s) 759, 997
PspGI CCWGG 2 cut(s) 759, 997
PspN4I GGNNCC 5 cut(s) 27, 313, 646, 928, 1003
PspPI GGNCC 2 cut(s) 631, 1001
PspPPI RGGWCCY 1 cut(s) 1001
PstI CTGCAG 1 cut(s) 430
PsuI RGATCY 1 cut(s) 1134
RsaI GTAC 2 cut(s) 215, 928
RsaNI GTAC 2 cut(s) 214, 927
RseI CAYNNNNRTG 1 cut(s) 960
SapI GCTCTTC 1 cut(s) 307
SaqAI TTAA 1 cut(s) 719
SatI GCNGC 1 cut(s) 426
Sau3AI GATC 5 cut(s) 404, 616, 1094, 1124, 1134
Sau96I GGNCC 2 cut(s) 631, 1001
ScaI AGTACT 1 cut(s) 215
SchI GAGTC 5 cut(s) 53, 263, 488, 635, 802
ScrFI CCNGG 2 cut(s) 761, 999
SduI GDGCHC 1 cut(s) 647
SfaNI GCATC 1 cut(s) 730
SfcI CTRYAG 2 cut(s) 426, 564
SfuI TTCGAA 1 cut(s) 970
SgrAI CRCCGGYG 1 cut(s) 199
SinI GGWCC 2 cut(s) 631, 1001
SmiMI CAYNNNNRTG 1 cut(s) 960
SmlI CTYRAG 3 cut(s) 623, 718, 832
SmoI CTYRAG 3 cut(s) 623, 718, 832
Sse9I AATT 4 cut(s) 129, 436, 754, 939
SsiI CCGC 4 cut(s) 161, 334, 930, 1138
SspMI CTAG 3 cut(s) 422, 797, 1165
StyD4I CCNGG 2 cut(s) 759, 997
StyI CCWWGG 3 cut(s) 9, 138, 1006
TaiI ACGT 2 cut(s) 76, 123
TaqI TCGA 1 cut(s) 970
TaqII GACCGA 1 cut(s) 619
TasI AATT 4 cut(s) 129, 436, 754, 939
TatI WGTACW 1 cut(s) 213
Tru1I TTAA 1 cut(s) 719
Tru9I TTAA 1 cut(s) 719
TscAI CASTG 2 cut(s) 189, 880
TseFI GTSAC 1 cut(s) 202
TseI GCWGC 1 cut(s) 425
Tsp45I GTSAC 1 cut(s) 202
TspDTI ATGAA 2 cut(s) 39, 517
TspGWI ACGGA 1 cut(s) 324
TspRI CASTG 2 cut(s) 189, 880
Vha464I CTTAAG 1 cut(s) 718
VpaK11BI GGWCC 2 cut(s) 631, 1001
XapI RAATTY 1 cut(s) 754
XceI RCATGY 2 cut(s) 287, 997
XcmI CCANNNNNNNNNTGG 3 cut(s) 104, 658, 1019
XspI CTAG 3 cut(s) 422, 797, 1165
ZrmI AGTACT 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.