RchiOBHm_Chr6g0293751

WD repeat-containing protein 89 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0009953
Physical Location & Seq
Reverse (-)
56290737 .. 56291609
873 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26359

Sequence Viewer

Length: 423 bp
ATGGAAGCCATGGACTTGGATATGGAATCGAAACCTCAACCGCAGGAGTCTCACTCCAATCCAAACTCATCCAAGCGTTTCGGCCTCAAAACCTCTATCCCAACCAACTTTGGAGACGATTACGTTTTCCAAATTGTGCCCAAGGATGATTGGACAGCAATGGCGGTGTCACTGTCAACCAACGCAGTGAAGCTCTACTCACCGGTGACTGGTCAGTACTACGGAGAGTGCAAAGGCCACTCTGCAACTATCAATCAAATTTCATTTGCAGACCCATCATCCCCACATGTTTTGCACTCCTGCTCTTCCGACGGAACCATTAGACTCTGGGACTCCCGCACATTCCAACAGGTTCGGTTCTCCATTCGCATTTGCATAGAGCAGCTTACTTCTGGGCATGTCTTTTCATTCTGGTTCGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010029 GO:0040008 GO:0048580 GO:0050789 GO:0050793 GO:0051239 GO:0065007 GO:1900140 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

140

Amino Acids

15.77

Weight (kDa)

5.66

Isoelectric Point (pI)

40.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 42 - 113 5.7e-07 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 42 - 119 8.8e-06 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 52 - 119 1e-08 WDR5 beta-propeller domain
Beta-prop_WDR75_1st PF23869 56 - 112 7e-07 WD repeat-containing protein 75 first beta-propeller
Beta-prop_THOC3 PF25174 60 - 118 3.1e-09 THOC3 beta-propeller domain
WD40_Prp19 PF24814 62 - 115 6e-06 Prp19 WD40 domain
WD40 PF00400 74 - 111 8e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 41, 164, 337
AcsI RAATTY 1 cut(s) 258
AfaI GTAC 1 cut(s) 218
AfiI CCNNNNNNNGG 1 cut(s) 209
AflIII ACRYGT 1 cut(s) 286
AgeI ACCGGT 1 cut(s) 202
AluBI AGCT 2 cut(s) 193, 385
AluI AGCT 2 cut(s) 193, 385
Alw26I GTCTC 2 cut(s) 54, 108
AoxI GGCC 2 cut(s) 82, 235
ApeKI GCWGC 1 cut(s) 382
ApoI RAATTY 1 cut(s) 258
AsiGI ACCGGT 1 cut(s) 202
AsuHPI GGTGA 2 cut(s) 192, 217
BaeGI GKGCMC 1 cut(s) 141
BbvI GCAGC 1 cut(s) 394
BccI CCATC 1 cut(s) 283
BcoDI GTCTC 2 cut(s) 54, 108
BisI GCNGC 1 cut(s) 383
BlsI GCNGC 1 cut(s) 384
BmcAI AGTACT 1 cut(s) 218
BmiI GGNNCC 1 cut(s) 316
BplI GAGNNNNNCTC 2 cut(s) 38, 70
BsaJI CCNNGG 2 cut(s) 9, 141
BsaWI WCCGGW 1 cut(s) 202
Bsc4I CCNNNNNNNGG 1 cut(s) 209
Bse118I RCCGGY 1 cut(s) 202
Bse1I ACTGG 1 cut(s) 214
Bse3DI GCAATG 1 cut(s) 165
BseDI CCNNGG 2 cut(s) 9, 141
BseGI GGATG 3 cut(s) 68, 151, 278
BseLI CCNNNNNNNGG 1 cut(s) 209
BseMI GCAATG 1 cut(s) 165
BseNI ACTGG 1 cut(s) 214
BseSI GKGCMC 1 cut(s) 141
BseXI GCAGC 1 cut(s) 394
BshFI GGCC 2 cut(s) 84, 237
BshTI ACCGGT 1 cut(s) 202
BsiSI CCGG 1 cut(s) 203
BslFI GGGAC 1 cut(s) 344
BslI CCNNNNNNNGG 1 cut(s) 209
BsmAI GTCTC 2 cut(s) 54, 108
BsmBI CGTCTC 1 cut(s) 108
BsmFI GGGAC 1 cut(s) 344
BsnI GGCC 2 cut(s) 84, 237
Bsp1286I GDGCHC 1 cut(s) 141
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 3 cut(s) 41, 164, 337
BspANI GGCC 2 cut(s) 84, 237
BspLI GGNNCC 1 cut(s) 316
BspQI GCTCTTC 1 cut(s) 310
BsrDI GCAATG 1 cut(s) 165
BsrFI RCCGGY 1 cut(s) 202
BsrI ACTGG 1 cut(s) 214
BssAI RCCGGY 1 cut(s) 202
BssECI CCNNGG 2 cut(s) 9, 141
BssT1I CCWWGG 2 cut(s) 9, 141
Bst4CI ACNGT 1 cut(s) 174
Bst6I CTCTTC 1 cut(s) 310
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 3 cut(s) 68, 151, 278
BstMAI GTCTC 2 cut(s) 54, 108
BstNSI RCATGY 2 cut(s) 290, 401
BstSLI GKGCMC 1 cut(s) 141
BstV1I GCAGC 1 cut(s) 394
BstXI CCANNNNNNTGG 1 cut(s) 16
BsuRI GGCC 2 cut(s) 84, 237
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 3 cut(s) 68, 151, 278
BtsI GCAGTG 1 cut(s) 192
BtsIMutI CAGTG 2 cut(s) 170, 192
Cfr10I RCCGGY 1 cut(s) 202
Csp6I GTAC 1 cut(s) 217
CspAI ACCGGT 1 cut(s) 202
CspCI CAANNNNNGTGG 2 cut(s) 273, 308
CviAII CATG 3 cut(s) 10, 287, 398
CviJI RGCY 5 cut(s) 8, 84, 193, 237, 385
CviKI_1 RGCY 5 cut(s) 8, 84, 193, 237, 385
CviQI GTAC 1 cut(s) 217
Eam1104I CTCTTC 1 cut(s) 310
EarI CTCTTC 1 cut(s) 310
Eco130I CCWWGG 2 cut(s) 9, 141
EcoT14I CCWWGG 2 cut(s) 9, 141
ErhI CCWWGG 2 cut(s) 9, 141
Esp3I CGTCTC 1 cut(s) 108
FaeI CATG 3 cut(s) 13, 290, 401
FaiI YATR 5 cut(s) 11, 23, 288, 377, 399
FaqI GGGAC 1 cut(s) 344
FatI CATG 3 cut(s) 9, 286, 397
FauI CCCGC 1 cut(s) 344
Fnu4HI GCNGC 1 cut(s) 383
FokI GGATG 3 cut(s) 55, 158, 265
Fsp4HI GCNGC 1 cut(s) 383
GluI GCNGC 1 cut(s) 383
HaeIII GGCC 2 cut(s) 84, 237
HapII CCGG 1 cut(s) 203
Hin1II CATG 3 cut(s) 13, 290, 401
HincII GTYRAC 1 cut(s) 177
HindII GTYRAC 1 cut(s) 177
HinfI GANTC 4 cut(s) 26, 47, 324, 332
HpaII CCGG 1 cut(s) 203
HphI GGTGA 2 cut(s) 192, 217
Hpy166II GTNNAC 1 cut(s) 177
Hpy188I TCNGA 1 cut(s) 310
Hpy8I GTNNAC 1 cut(s) 177
Hpy99I CGWCG 1 cut(s) 314
HpyCH4III ACNGT 1 cut(s) 174
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 5 cut(s) 231, 245, 269, 295, 375
HpySE526I ACGT 1 cut(s) 123
Hsp92II CATG 3 cut(s) 13, 290, 401
LguI GCTCTTC 1 cut(s) 310
LpnPI CCDG 8 cut(s) 29, 195, 216, 313, 313, 335, 378, 397
Lsp1109I GCAGC 1 cut(s) 394
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 2 cut(s) 168, 205
MboII GAAGA 1 cut(s) 297
MhlI GDGCHC 1 cut(s) 141
MluCI AATT 2 cut(s) 132, 258
MlyI GAGTC 3 cut(s) 56, 318, 326
MmeI TCCRAC 2 cut(s) 333, 370
MnlI CCTC 3 cut(s) 45, 95, 103
MspI CCGG 1 cut(s) 203
NcoI CCATGG 1 cut(s) 9
NlaIII CATG 3 cut(s) 13, 290, 401
NlaIV GGNNCC 1 cut(s) 316
NmuCI GTSAC 2 cut(s) 168, 205
NspI RCATGY 2 cut(s) 290, 401
PciI ACATGT 1 cut(s) 286
PciSI GCTCTTC 1 cut(s) 310
PfeI GAWTC 1 cut(s) 26
PinAI ACCGGT 1 cut(s) 202
PkrI GCNGC 1 cut(s) 384
PleI GAGTC 3 cut(s) 55, 318, 326
PpsI GAGTC 3 cut(s) 55, 318, 326
PscI ACATGT 1 cut(s) 286
PspN4I GGNNCC 1 cut(s) 316
RsaI GTAC 1 cut(s) 218
RsaNI GTAC 1 cut(s) 217
SapI GCTCTTC 1 cut(s) 310
SatI GCNGC 1 cut(s) 383
ScaI AGTACT 1 cut(s) 218
SchI GAGTC 3 cut(s) 56, 318, 326
SduI GDGCHC 1 cut(s) 141
SetI ASST 6 cut(s) 37, 95, 126, 195, 354, 387
SgrAI CRCCGGYG 1 cut(s) 202
Sse9I AATT 2 cut(s) 132, 258
SsiI CCGC 3 cut(s) 41, 164, 337
StyI CCWWGG 2 cut(s) 9, 141
TaaI ACNGT 1 cut(s) 174
TaiI ACGT 1 cut(s) 126
TaqI TCGA 2 cut(s) 29, 417
TasI AATT 2 cut(s) 132, 258
TatI WGTACW 1 cut(s) 216
TfiI GAWTC 1 cut(s) 26
TscAI CASTG 2 cut(s) 177, 192
TseFI GTSAC 2 cut(s) 168, 205
TseI GCWGC 1 cut(s) 382
Tsp45I GTSAC 2 cut(s) 168, 205
TspDTI ATGAA 2 cut(s) 252, 396
TspGWI ACGGA 2 cut(s) 237, 327
TspRI CASTG 2 cut(s) 177, 192
XapI RAATTY 1 cut(s) 258
XceI RCATGY 2 cut(s) 290, 401
ZrmI AGTACT 1 cut(s) 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.