Rorug06G0237800

WD repeat-containing protein 89 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0009953
Physical Location & Seq
Reverse (-)
38031454 .. 38033154
1701 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0237800.1

Sequence Viewer

Length: 1167 bp
ATGGAAAACAAATGGGTTCTTCCTCTAGCCCTATCCTTGTTTATAACTGTTCTTAGCTTGGCAGCAGCAGCTAAGCCAGTACTGCCACCAGTCTTCATATTTGGTGATTCAACAGCAGATGTCGGCACCAATAACTTCTTGCCGGCTAGCAAGGCAAGAGCTGACTTTCCCCCCAATGGAATTGACTTTCCTCGCACCTCAAAACCCACCGGAAGGTTCAGCAATGGCCTTAACAGTGCTGATTTTCTTGCCCGGCATTTAGGTTACAAGAGAAGTCCAAGCCCCTTTCTTTCTCTGAAAACTACTTCTCTTCAAAAGAAGAAGTTTAATGGTATCAACTTTGCTTCTGGAGGGTCTGGTCTTCTTGACATAACTGGACACACAATGGCAAGTCACTTTCTTGGGGAACAACTGACATTGATGAAGTTTGGAACTGGAAATATTCCATTTGGTGCCTCATTCAAAAACCAGAAAAAAGTTATCTCATTAACAGAGCAGATACAGCAATTTTCATCTGTCAAGAACAATCTTACGGCCTTGATGGGTGCGGTAGCAACTGAGAAGTTTCTTAAAGAGTCTTTGATCTTCATCAGCACCGGCAGCAATGACCTTTTCGGATACTACCATTCAAAGAGTTCCATTCCGAAGGAAAAGTTCTTGTCCTCTTTAGAAGTAGCTTATGAGAATCATTTGAAGACTCTATACAATCTTGGAGCAAGGAAGTTTGGCATCATCAGCATTGCCCCGATTGGCTGCTGCCCATCTCAGAGGATTTTCAATGCTACTGGGGGATGTTTGGAAGAGCTGAATGATAATGCAATAGCTTTTCATTCAAGATTGGATGCCCTCTTATGCAAGCTTAGCTCAGAATACAAGGGCATGAAGTACTCACTTGGAAATTCATATGAAATGACAATCAATGTCATACAAAACCCTCTTCCATTCAATTTTACACAAGTGGCAGCTGCATGTTGTGGAACTGGGAAGCTCAATGCTGAAAACTTCTGTAAATCAGATGAAAATCTCTGTTCGAATCGCCATCAATACTTGTTCTGGGATCGATTTCATCCAACACAGGCTGCTTCTAAGTTGGCTGCTGTTGCCCTCTACAGTGGTGGACCACAATTTGTATCCCCGATTAATTTTTCTCAGTTGGCCAAGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010029 GO:0040008 GO:0048580 GO:0050789 GO:0050793 GO:0051239 GO:0065007 GO:1900140 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

388

Amino Acids

42.25

Weight (kDa)

9.32

Isoelectric Point (pI)

24.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 31 - 366 6.5e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 44
AccB1I GGYRCC 2 cut(s) 125, 452
AciI CCGC 1 cut(s) 548
AclWI GGATC 1 cut(s) 1065
AcoI YGGCCR 1 cut(s) 1155
AcsI RAATTY 1 cut(s) 898
AfaI GTAC 2 cut(s) 81, 887
AfiI CCNNNNNNNGG 2 cut(s) 176, 213
AgsI TTSAA 8 cut(s) 111, 314, 463, 630, 694, 778, 834, 946
AlwI GGATC 1 cut(s) 1065
AoxI GGCC 3 cut(s) 226, 534, 1155
ApoI RAATTY 1 cut(s) 898
AseI ATTAAT 1 cut(s) 1140
AspS9I GGNCC 1 cut(s) 1118
AsuC2I CCSGG 1 cut(s) 253
AsuHPI GGTGA 1 cut(s) 116
AsuII TTCGAA 1 cut(s) 1031
AsuNHI GCTAGC 1 cut(s) 146
AvaII GGWCC 1 cut(s) 1118
BalI TGGCCA 1 cut(s) 1157
BanI GGYRCC 2 cut(s) 125, 452
BarI GAAGNNNNNNTAC 2 cut(s) 686, 718
BbsI GAAGAC 3 cut(s) 85, 353, 701
BccI CCATC 3 cut(s) 535, 769, 1047
BceAI ACGGC 1 cut(s) 549
BciVI GTATCC 2 cut(s) 611, 1141
BcnI CCSGG 1 cut(s) 253
BfaI CTAG 2 cut(s) 26, 147
BfmI CTRYAG 1 cut(s) 1108
BfuI GTATCC 2 cut(s) 611, 1141
BlpI GCTNAGC 2 cut(s) 72, 860
BmcAI AGTACT 2 cut(s) 81, 887
Bme1390I CCNGG 1 cut(s) 253
Bme18I GGWCC 1 cut(s) 1118
BmgT120I GGNCC 1 cut(s) 1118
BmiI GGNNCC 2 cut(s) 127, 454
BmrFI CCNGG 1 cut(s) 253
BmrI ACTGGG 2 cut(s) 795, 990
BmsI GCATC 2 cut(s) 738, 832
BmtI GCTAGC 1 cut(s) 150
BmuI ACTGGG 2 cut(s) 795, 990
BpiI GAAGAC 3 cut(s) 85, 353, 701
BpmI CTGGAG 1 cut(s) 369
Bpu1102I GCTNAGC 2 cut(s) 72, 860
Bpu14I TTCGAA 1 cut(s) 1031
BpuMI CCSGG 1 cut(s) 253
Bsa29I ATCGAT 1 cut(s) 1060
BsaBI GATNNNNATC 2 cut(s) 587, 1020
BsaJI CCNNGG 1 cut(s) 1158
BsaWI WCCGGW 1 cut(s) 209
Bsc4I CCNNNNNNNGG 2 cut(s) 176, 213
Bse118I RCCGGY 2 cut(s) 142, 596
Bse1I ACTGG 6 cut(s) 77, 89, 379, 439, 790, 985
Bse3DI GCAATG 3 cut(s) 229, 610, 738
Bse8I GATNNNNATC 2 cut(s) 587, 1020
BseCI ATCGAT 1 cut(s) 1060
BseDI CCNNGG 1 cut(s) 1158
BseGI GGATG 3 cut(s) 797, 847, 1066
BseJI GATNNNNATC 2 cut(s) 587, 1020
BseLI CCNNNNNNNGG 2 cut(s) 176, 213
BseMI GCAATG 3 cut(s) 229, 610, 738
BseMII CTCAG 4 cut(s) 549, 779, 879, 1163
BseNI ACTGG 6 cut(s) 77, 89, 379, 439, 790, 985
BshFI GGCC 3 cut(s) 228, 536, 1157
BshNI GGYRCC 2 cut(s) 125, 452
BshVI ATCGAT 1 cut(s) 1060
BsiSI CCGG 4 cut(s) 143, 210, 253, 597
BslI CCNNNNNNNGG 2 cut(s) 176, 213
BsnI GGCC 3 cut(s) 228, 536, 1157
Bsp119I TTCGAA 1 cut(s) 1031
Bsp143I GATC 2 cut(s) 582, 1057
Bsp1720I GCTNAGC 2 cut(s) 72, 860
BspACI CCGC 1 cut(s) 548
BspANI GGCC 3 cut(s) 228, 536, 1157
BspCNI CTCAG 4 cut(s) 550, 778, 878, 1162
BspDI ATCGAT 1 cut(s) 1060
BspLI GGNNCC 2 cut(s) 127, 454
BspOI GCTAGC 1 cut(s) 150
BspPI GGATC 1 cut(s) 1065
BspQI GCTCTTC 1 cut(s) 795
BspT104I TTCGAA 1 cut(s) 1031
BspT107I GGYRCC 2 cut(s) 125, 452
BsrDI GCAATG 3 cut(s) 229, 610, 738
BsrFI RCCGGY 2 cut(s) 142, 596
BsrI ACTGG 6 cut(s) 77, 89, 379, 439, 790, 985
BssAI RCCGGY 2 cut(s) 142, 596
BssECI CCNNGG 1 cut(s) 1158
BssMI GATC 2 cut(s) 582, 1057
BssT1I CCWWGG 1 cut(s) 1158
Bst4CI ACNGT 3 cut(s) 49, 236, 1112
Bst6I CTCTTC 3 cut(s) 315, 795, 942
BstBI TTCGAA 1 cut(s) 1031
BstC8I GCNNGC 3 cut(s) 144, 148, 857
BstDEI CTNAG 9 cut(s) 53, 72, 558, 765, 860, 865, 1086, 1149, 1164
BstF5I GGATG 3 cut(s) 797, 847, 1066
BstKTI GATC 2 cut(s) 585, 1060
BstMBI GATC 2 cut(s) 582, 1057
BstMWI GCNNNNNNNGC 8 cut(s) 68, 82, 152, 502, 600, 735, 861, 1100
BstNSI RCATGY 1 cut(s) 972
BstSCI CCNGG 1 cut(s) 251
BstSFI CTRYAG 1 cut(s) 1108
BstV2I GAAGAC 3 cut(s) 85, 353, 701
Bsu15I ATCGAT 1 cut(s) 1060
BsuI GTATCC 2 cut(s) 611, 1141
BsuRI GGCC 3 cut(s) 228, 536, 1157
BsuTUI ATCGAT 1 cut(s) 1060
BtsCI GGATG 3 cut(s) 797, 847, 1066
BtsIMutI CAGTG 2 cut(s) 241, 1117
Cac8I GCNNGC 3 cut(s) 144, 148, 857
Cfr10I RCCGGY 2 cut(s) 142, 596
Cfr13I GGNCC 1 cut(s) 1118
ClaI ATCGAT 1 cut(s) 1060
Csp6I GTAC 2 cut(s) 80, 886
CviAII CATG 2 cut(s) 880, 969
CviQI GTAC 2 cut(s) 80, 886
DdeI CTNAG 9 cut(s) 53, 72, 558, 765, 860, 865, 1086, 1149, 1164
DpnI GATC 2 cut(s) 584, 1059
DpnII GATC 2 cut(s) 582, 1057
EaeI YGGCCR 1 cut(s) 1155
Eam1104I CTCTTC 3 cut(s) 315, 795, 942
EarI CTCTTC 3 cut(s) 315, 795, 942
Eco130I CCWWGG 1 cut(s) 1158
Eco47I GGWCC 1 cut(s) 1118
EcoT14I CCWWGG 1 cut(s) 1158
ErhI CCWWGG 1 cut(s) 1158
FaeI CATG 2 cut(s) 883, 972
FatI CATG 2 cut(s) 879, 968
FauNDI CATATG 1 cut(s) 904
FokI GGATG 3 cut(s) 804, 854, 1053
FspBI CTAG 2 cut(s) 26, 147
GsuI CTGGAG 1 cut(s) 369
HaeIII GGCC 3 cut(s) 228, 536, 1157
HapII CCGG 4 cut(s) 143, 210, 253, 597
Hin1II CATG 2 cut(s) 883, 972
HindIII AAGCTT 1 cut(s) 857
HinfI GANTC 5 cut(s) 107, 575, 685, 697, 1033
HpaII CCGG 4 cut(s) 143, 210, 253, 597
HphI GGTGA 1 cut(s) 116
Hpy166II GTNNAC 1 cut(s) 1118
Hpy188I TCNGA 6 cut(s) 297, 617, 645, 768, 868, 1015
Hpy188III TCNNGA 4 cut(s) 348, 365, 520, 834
Hpy8I GTNNAC 1 cut(s) 1118
HpyAV CCTTC 2 cut(s) 207, 640
HpyCH4III ACNGT 3 cut(s) 49, 236, 1112
HpyCH4V TGCA 3 cut(s) 818, 855, 968
HpyF10VI GCNNNNNNNGC 8 cut(s) 68, 82, 152, 502, 600, 735, 861, 1100
HpyF3I CTNAG 9 cut(s) 53, 72, 558, 765, 860, 865, 1086, 1149, 1164
Hsp92II CATG 2 cut(s) 883, 972
KroI GCCGGC 1 cut(s) 142
KroNI GCCGGC 1 cut(s) 144
Kzo9I GATC 2 cut(s) 582, 1057
LguI GCTCTTC 1 cut(s) 795
LmnI GCTCC 1 cut(s) 713
LweI GCATC 2 cut(s) 738, 832
MaeI CTAG 2 cut(s) 26, 147
MaeIII GTNAC 2 cut(s) 263, 392
MalI GATC 2 cut(s) 584, 1059
MboI GATC 2 cut(s) 582, 1057
MboII GAAGA 9 cut(s) 11, 85, 302, 331, 353, 577, 706, 812, 929
MlsI TGGCCA 1 cut(s) 1157
MluCI AATT 6 cut(s) 180, 506, 898, 946, 1124, 1141
MluNI TGGCCA 1 cut(s) 1157
MlyI GAGTC 2 cut(s) 584, 691
MmeI TCCRAC 1 cut(s) 1094
Mox20I TGGCCA 1 cut(s) 1157
MroNI GCCGGC 1 cut(s) 142
MscI TGGCCA 1 cut(s) 1157
MseI TTAA 5 cut(s) 231, 327, 488, 570, 1140
Msp20I TGGCCA 1 cut(s) 1157
MspA1I CMGCKG 1 cut(s) 965
MspI CCGG 4 cut(s) 143, 210, 253, 597
MspR9I CCNGG 1 cut(s) 253
MwoI GCNNNNNNNGC 8 cut(s) 68, 82, 152, 502, 600, 735, 861, 1100
NaeI GCCGGC 1 cut(s) 144
NciI CCSGG 1 cut(s) 253
NdeI CATATG 1 cut(s) 904
NdeII GATC 2 cut(s) 582, 1057
NgoMIV GCCGGC 1 cut(s) 142
NheI GCTAGC 1 cut(s) 146
NlaIII CATG 2 cut(s) 883, 972
NlaIV GGNNCC 2 cut(s) 127, 454
NmuCI GTSAC 1 cut(s) 392
NspI RCATGY 1 cut(s) 972
NspV TTCGAA 1 cut(s) 1031
PciSI GCTCTTC 1 cut(s) 795
PdiI GCCGGC 1 cut(s) 144
PfeI GAWTC 3 cut(s) 107, 685, 1033
PleI GAGTC 2 cut(s) 583, 691
PpsI GAGTC 2 cut(s) 583, 691
PshBI ATTAAT 1 cut(s) 1140
PsiI TTATAA 1 cut(s) 44
PspN4I GGNNCC 2 cut(s) 127, 454
PspPI GGNCC 1 cut(s) 1118
PvuII CAGCTG 1 cut(s) 965
RsaI GTAC 2 cut(s) 81, 887
RsaNI GTAC 2 cut(s) 80, 886
SapI GCTCTTC 1 cut(s) 795
SaqAI TTAA 5 cut(s) 231, 327, 488, 570, 1140
Sau3AI GATC 2 cut(s) 582, 1057
Sau96I GGNCC 1 cut(s) 1118
ScaI AGTACT 2 cut(s) 81, 887
SchI GAGTC 2 cut(s) 584, 691
ScrFI CCNGG 1 cut(s) 253
SfaNI GCATC 2 cut(s) 738, 832
SfcI CTRYAG 1 cut(s) 1108
SfuI TTCGAA 1 cut(s) 1031
SinI GGWCC 1 cut(s) 1118
Sse9I AATT 6 cut(s) 180, 506, 898, 946, 1124, 1141
SsiI CCGC 1 cut(s) 548
SspI AATATT 1 cut(s) 442
SspMI CTAG 2 cut(s) 26, 147
StyD4I CCNGG 1 cut(s) 251
StyI CCWWGG 1 cut(s) 1158
TaaI ACNGT 3 cut(s) 49, 236, 1112
TaqI TCGA 2 cut(s) 1031, 1060
TasI AATT 6 cut(s) 180, 506, 898, 946, 1124, 1141
TatI WGTACW 2 cut(s) 79, 885
TfiI GAWTC 3 cut(s) 107, 685, 1033
Tru1I TTAA 5 cut(s) 231, 327, 488, 570, 1140
Tru9I TTAA 5 cut(s) 231, 327, 488, 570, 1140
TscAI CASTG 2 cut(s) 241, 1117
TseFI GTSAC 1 cut(s) 392
Tsp45I GTSAC 1 cut(s) 392
TspRI CASTG 2 cut(s) 241, 1117
VpaK11BI GGWCC 1 cut(s) 1118
VspI ATTAAT 1 cut(s) 1140
XapI RAATTY 1 cut(s) 898
XceI RCATGY 1 cut(s) 972
XspI CTAG 2 cut(s) 26, 147
ZrmI AGTACT 2 cut(s) 81, 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.